KR20220128644A - 게놈 변형을 위한 높은 충실도 SpCas9 뉴클라제 - Google Patents

게놈 변형을 위한 높은 충실도 SpCas9 뉴클라제 Download PDF

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KR20220128644A
KR20220128644A KR1020227027960A KR20227027960A KR20220128644A KR 20220128644 A KR20220128644 A KR 20220128644A KR 1020227027960 A KR1020227027960 A KR 1020227027960A KR 20227027960 A KR20227027960 A KR 20227027960A KR 20220128644 A KR20220128644 A KR 20220128644A
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KR1020227027960A
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푸치앙 첸
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시그마-알드리치 컴퍼니., 엘엘씨
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Publication of KR20220128644A publication Critical patent/KR20220128644A/ko

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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00Nucleic acids vectors
    • C12N2800/22Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Bioinformatics & Cheminformatics (AREA)
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  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
KR1020227027960A 2020-03-11 2021-03-11 게놈 변형을 위한 높은 충실도 SpCas9 뉴클라제 KR20220128644A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062988279P 2020-03-11 2020-03-11
US62/988,279 2020-03-11
PCT/US2021/021922 WO2021183771A1 (en) 2020-03-11 2021-03-11 High fidelity spcas9 nucleases for genome modification

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KR20220128644A true KR20220128644A (ko) 2022-09-21

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KR1020227027960A KR20220128644A (ko) 2020-03-11 2021-03-11 게놈 변형을 위한 높은 충실도 SpCas9 뉴클라제

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Country Link
US (1) US20230058352A1 (he)
EP (1) EP4118197A1 (he)
JP (1) JP2023514327A (he)
KR (1) KR20220128644A (he)
CN (1) CN115244177A (he)
AU (1) AU2021236230A1 (he)
BR (1) BR112022012350A2 (he)
CA (1) CA3163463A1 (he)
IL (1) IL294120B2 (he)
WO (1) WO2021183771A1 (he)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023077149A1 (en) 2021-11-01 2023-05-04 Sigma-Aldrich Co. Llc Electroporation enhancers for crispr-cas systems
CN117866926A (zh) * 2024-03-07 2024-04-12 珠海舒桐医疗科技有限公司 一种CRISPR-FrCas9蛋白突变体及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA04005010A (es) 2001-11-26 2005-04-08 Advanced Cell Tech Inc METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS.
CA2989830A1 (en) * 2015-06-18 2016-12-22 The Broad Institute, Inc. Crispr enzyme mutations reducing off-target effects
US11242542B2 (en) * 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same

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Publication number Publication date
EP4118197A1 (en) 2023-01-18
IL294120A (he) 2022-08-01
CA3163463A1 (en) 2021-09-16
CN115244177A (zh) 2022-10-25
US20230058352A1 (en) 2023-02-23
AU2021236230A1 (en) 2022-06-30
IL294120B1 (he) 2023-06-01
BR112022012350A2 (pt) 2022-09-27
JP2023514327A (ja) 2023-04-05
WO2021183771A1 (en) 2021-09-16
IL294120B2 (he) 2023-10-01

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