KR20220126123A - Ignatzschineria strain producing auxin and promoting plant growth and uses thereof - Google Patents

Abstract

The present invention relates to an Ignatzschineria strain which promotes the growth of plants by promoting auxin production in the plants, and uses thereof. The Ignatzschineria strain of the present invention can significantly promote the growth of plants, preferably the root growth of plants. In addition, the Ignatzschineria strain or a culture solution thereof of the present invention can replace expensive imported products or chemical fertilizers on the market for root growth.

Description

Ignatzineria strain producing auxin and promoting plant growth and uses thereof to produce auxin and promote plant growth and uses thereof

The present invention relates to a novel Ignacineria strain that promotes plant growth by promoting auxin production in plants and uses thereof.

Plants live in close interaction with the microbes around them, and these interactions affect overall plant life cycle and health. Plants provide nutrients and shelter for the surrounding microorganisms to survive. Beneficial microorganisms secrete substances that promote plant growth, make it easier for plants to absorb nutrients, and help improve resistance to environmental stress and pathogens (O'Banion et al. (2020) Bridging the The gap between single-strain and community-level plant-microbe chemical interactions. MPMI 33(2):124-134; Vimal et al. (2017) Soil-plant-microbe interactions in stressed agriculture management: a review. Pedosphere. 27( 2):177-192). Microorganisms that promote plant growth through these beneficial actions around the roots of plants are called Plant Growth-Promoting Rhizobacteria (PGPR).

Among the PGPRs, there are microorganisms that promote plant growth by secreting auxin or cytokinin, which are plant growth hormones. These microorganisms include Agrobacterium , Azospirillum, Bacillus , Bradyrhizobium , Enterobacter cloacae , Pseudomonas , Paenibacillus , Pseudomonas . , Rhizobium , and various microorganisms are known (Spaepen and Vanderleyden (2011) Auxin and plant-microbe interactions. Cold Spring Harb Perspect Biol. 3:a001438). In particular, auxin is a major signaling material in the interaction between microorganisms and plants, and is a hormone that induces root development of plants.

In the prior art, there are research results on microorganisms that promote root growth of plants, and the effect of these microorganisms on generating auxins is already known. For example, Korean Patent Registration No. 10-1456171 discloses auxin production and plant growth promoting effects such as Tumebacillus sp. strain, Bacillus sp. strain, Agrobacterium sp. strain, and Racina bacillus sp. strain isolated from water pampas grass roots. has been However, there are few cases in which these microorganisms have been developed as products for promoting rooting or root growth. On the other hand, Ignatzneria ( Ignatzschineria ) strain isolated and identified in the present invention is a microorganism that is not known as PGPR. In addition, the interaction with plants of the Ignacineria strain, or properties related to plant growth are not yet known, and its pathogenicity has not been reported either.

Synthetic hormone products marketed for promoting root growth include, for example, Luton (active ingredient: Naphtol Alphanol Acid). In addition, natural extract products include, for example, Roots α+ (active ingredient: Ascophyllum nodosum extract). In addition, the trace element complex fertilizer that helps the roots to grow healthy includes, for example, rootgro (active ingredient: boron 0.05%, molybdenum 0.0005%), and the like, and humic acid that promotes plant health.

As described above, products containing natural extracts or inorganic fertilizers that enhance the root development of plants are commercially available, but there are few microbial preparations. In addition, some previously known microorganisms are known to have an effect of promoting root development, but the effect is rather weak to actually commercialize them. Therefore, there is a need for the development of a novel microbial agent having a remarkably excellent effect of promoting plant growth.

Domestic Registered Patent No. 10-1456171 (Announcement on October 31, 2014)

O'Banion et al. (2020) Bridging the gap between single-strain and community-level plant-microbe chemical interactions. MPMI 33(2):124-134 Vimal et al. (2017) Soil-plant-microbe interactions in stressed agriculture management: a review. Pedosphere. 27(2):177-192 Spaepen and Vanderleyden (2011) Auxin and plant-microbe interactions. Cold Spring Harb Perspect Biol. 3:a001438

Therefore, the problem to be solved by the present invention is to provide a novel microorganism strain excellent in the effect of producing auxin and promoting the growth of plants.

In addition, the problem to be solved by the present invention is to provide a microbial preparation comprising the microbial strain or a culture solution thereof as an active ingredient, and a method for promoting plant growth using the same.

In order to solve the above problems, the inventors of the present invention made intensive research efforts to discover new microorganisms having excellent effects of promoting plant growth. Accordingly, the present inventors discovered a novel microorganism having an excellent auxin production effect from processing by-products of food materials used for the production of organic fertilizers, and as a result of analyzing its 16S rDNA nucleotide sequence, it was confirmed that it is a genus strain of Ignatzschineria . The inventors of the present invention confirmed that the auxin-producing effect of the Ignacineria sp. strain of the present invention, which has hardly been reported in biological properties, is remarkably superior to that of other auxin-producing bacteria known previously. In addition, the Ignacineria sp. strain made a surprising discovery that significantly promotes the growth of various plants, preferably the root growth of plants, thereby completing the present invention.

The present invention provides a CG200001 strain ( KCTC14213BP ) of the genus Ignatzschineria. The Ignacineria genus CG200001 strain was deposited with the Korea Research Institute of Bioscience and Biotechnology on June 12, 2020, and was given an accession number KCTC14213BP.

In the present invention, the 16S rDNA of the Ignacineria genus CG200001 strain (KCTC14213BP) is composed of the nucleotide sequence of SEQ ID NO: 1.

In the present invention, the Ignacineria genus CG200001 strain can promote the growth of plants, and preferably can significantly promote the formation of roots of plants. The Ignacineria genus CG200001 strain can remarkably produce auxin, preferably indole-3-acetic acid (IAA), which is a substance that promotes plant growth in plants, and through this, plants of growth, preferably root formation.

The CG200001 strain of the genus Ignacineria according to the present invention can be cultured in large quantities by a conventional culturing method of the genus Ignacineria. As the culture medium, a medium composed of a carbon source, a nitrogen source, vitamins and minerals may be used. For example, NB (nutrient broth), TSB (tryptic soy broth) liquid medium, or TSA (tryptic soy agar) solid medium may be used. Cultivation may be performed under normal culture conditions, for example, may be cultured at about 37° C. for about 10 to 72 hours. In order to remove the culture medium in the culture medium and recover only the concentrated cells, centrifugation or filtration may be performed, and these steps may be performed according to the needs of those skilled in the art. The concentrated cells can be frozen or freeze-dried according to a conventional method to preserve their activity so as not to lose their activity.

In addition, the CG200001 strain of the genus Ignacineria according to the present invention can be improved or improved to have an activity equivalent to or superior to this by a conventional physicochemical mutation method known in the art.

In addition, the present invention provides a composition for promoting plant growth comprising the Ignacineria genus CG200001 strain, a culture medium of the strain, or both as an active ingredient. In addition, the present invention provides an agricultural microbial preparation comprising the Ignacineria genus CG200001 strain, a culture solution of the strain, or both as an active ingredient. In one embodiment, the composition for promoting plant growth or an agricultural microbial agent may be used to promote root formation and growth of plants or seedlings.

In the composition for promoting plant growth or agricultural microbial preparations, the CG200001 strain of the genus Ignacineria is not limited to the strain itself, but the crushed cell wall fraction of the CG200001 strain, live cells, dead cells, dried bacteria, or a culture or culture solution thereof It can also be included in the form of In addition, the composition for promoting plant growth or an agricultural microbial preparation may further include a suitable excipient or carrier. The culture solution is the culture solution itself in which the strain according to the present invention is cultured in a suitable liquid medium, the filtrate (filtrate or centrifuged supernatant) from which the strain is removed by filtration or centrifugation of the culture solution, the culture solution is sonicated or dissolved in the culture solution It may include, but is not limited to, a cell lysate obtained by treatment with an enzyme (lysozyme), and the like. In addition, the composition for promoting plant growth or the microorganism preparation for agriculture may contain a culture such as a solid medium culture, or a dried product or an extract of the culture medium, but is not limited thereto.

In addition, the present invention is any one or more selected from the group consisting of the Ignacineria genus CG200001 strain, the culture medium of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain, the plant, the surrounding area of the plant, Or it provides a method for promoting plant growth comprising the step of treating them both. The plant species to which the plant growth promotion method can be used is not particularly limited, and for example, it may be used for seeds or plants such as pepper, rice, chrysanthemum, red kale, or basil.

Ignacineria strain of the present invention can significantly promote the growth of plants, preferably root growth of plants. In addition, the Ignacineria strain or its culture medium of the present invention can replace expensive imported products on the market for root growth use, and extracts (humic acid, fulvic acid, or Ascophyllum ( Ascophyllum nodosum ), etc.) can be reduced.

1a and 1b are results of comparing the 16s rDNA sequence of the Ignacineria CG 200001 strain of the present invention with the 16s rDNA sequence of the Ignacineria LJ11 strain (Genebank no.MG049766.1).
2 to 4 show changes in the IAA content in the culture medium according to the addition of a carbon source (glucose, starch) or a precursor of IAA (tryptophan).
Figure 5 shows the pepper plants rooted and established after 2 weeks of cuttings and treated with the Ignacineria strain culture of the present invention.
6 is a photograph of a plant having roots formed about a week after treatment of the Koshihikari rice seedlings with the culture medium of the Ignacineria strain of the present invention, and FIG. 7 shows the average value of the number of roots in each treatment group (FIG. 7) ).
8 is a comparison of the number of irregular roots formed after 2 weeks of cutting and processing the culture solution of the Ignacineria strain of the present invention to the chrysanthemum plant.
9 is an experimental result confirming the initial growth of rice when treating the culture solution of the Ignacineria strain of the present invention.
10 is a comparison of the yield of red kale with and without treatment with the culture medium of the Ignacineria strain of the present invention in broodstock or chicken fertilization.
11 is a comparison of the yield of basil in the case where the culture solution of the Ignacineria strain of the present invention was not treated and the case where the culture solution of the Ignacineria strain of the present invention was not treated in the broodstock, chicken manure fertilization or coffee compost fertilization.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art to which the present invention pertains.

Experimental Example 1: Ignition and identification of Ignacineria strains

The Ignacineria strain of the present invention was isolated from the raw material of the fermentation start stage while studying the change of the microbial community during the ripening process of organic fertilizer. Specifically, the strain was isolated from processing by-products of food materials collected for the production of organic fertilizers. After the by-product was diluted several times in sterile water, it was spread on an agar medium and single colonies were cultured. A bacterial clone producing a high content of auxin was isolated from among the single colonies using Salkowski's reagent.

After culturing the bacterial clones in LB broth, DNA of the bacteria was isolated using a DNA isolation kit (Exgene(TM) Cell SV mini, GeneAll). The 16s rRNA nucleotide sequence was amplified using the primer set shown in Table 1 below, and the nucleotide sequence of the PCR product of about 1400 bp was analyzed (Cosmogene Tech). The 16s rDNA base sequence of the Ignacineria strain of the present invention is shown as SEQ ID NO: 1.

Primer name order 27F (forward) 5'-AGAGTTTGATCMTGGCTCAG-3' (SEQ ID NO: 2) 1492R (reverse) 5'-GGTTACCTTGTTACGACTTC-3' (SEQ ID NO: 3)

The analyzed nucleotide sequence was compared with the nucleotide sequences of the Genebank database using the BLASTN search tool (http://blast.ncbi.nlm.nih.gov/Blast.cgi). As a result, the strain showed the highest similarity to the bacteria of the genus Ignatzschineria . Accordingly, the novel strain isolated in the present invention Ignatzneria ( Ignatzschineria ) sp. It was named CG 200001. Specifically, as a result of comparison with the Genebank database, the 16s rDNA sequence (SEQ ID NO: 1) of the Ignacineria CG 200001 strain of the present invention is the 16s rDNA sequence of the Ignacineria LJ11 strain (Genebank no. It was most similar to .MG049766.1, SEQ ID NO: 4), and it was confirmed that only two bases of their 1.4 kb nucleotide sequence were different from each other (shaded in FIGS. 1A and 1B ).

Experimental Example 2: Indole-3-acetic acid (IAA) production effect of Ignacineria strain

An experiment was performed to analyze the effect of IAA biosynthesis of the Ignacineria CG 200001 strain. First, a carbon source (glucose, starch) or a precursor of IAA (tryptophan) was added to the strain at different concentrations (0.5 g/L or 1 g/L), and cultured in 1/2 diluted LB. Each was centrifuged after 2 hours, 3.5 hours, or 5.5 hours from the start of the culture, and IAA in the culture supernatant containing no bacteria was quantified using the Salkowski method.

Salkowski reagent contains 1 ml of 0.5M FeCl ₃ and 49 ml of 35% perchloric acid based on 50 ml of the composition. Salkowski reagent reacts with IAA to develop color, and the colorant exhibits maximum absorption at 536 nm. The Salkowski analysis method used in this experiment was described in Szkop et al. (2012) A novel, simple, and sensitive colorimetric method to determine aromatic amino acid aminotransferase activity using the Salkowski reagent. Foli Microbiol. 57: 1-4; and Gang et al. (2019) Analysis of indole-3-acetic acid (IAA) production in Klebsiella by LC-MS/MS and the Salkowski method. Bio-protocol 9(09):e3230 was referenced.

Changes in the IAA content in the culture medium according to the addition of the carbon source (glucose, starch) or IAA precursor (tryptophan) are shown in FIGS. 2 to 4 . 4, in the treatment group to which 0.5 g/L tryptophan was added, the IAA content in the medium increased from 65 mg/L to 118.6 mg/L, and in the treatment group to which 1 g/L tryptophan was added, the IAA content was It increased from 102.6 mg/L to 170.5 mg/L, and it was found to produce up to 19.4 mg/L of IAA per hour.

This IAA production effect is remarkably superior to the IAA production effect of other microorganisms reported in previous studies. For example, a strain isolated from the rhizosphere of Stevia rebaudiana produces about 104 mg/L of IAA for 24 hours (Chandra et al. (2018), Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere) and its effects on plant growth, Journal of Genetic Engineering and Biotechnology 16:581-586), rhizosphere bacteria isolated from Button Mushroom compost produced about 109.9 mg/L of IAA for 24 hours (Oh et al. (2015), Isolation and Characterization of Plant Growth Promoting Rhizobacteria From Button Mushroom Compost, Korean Journal of Agricultural Science 43(1): 100-108).

Experimental Example 3: Root formation promoting effect on pepper plants of Ignacineria strain culture medium

In order to confirm whether the culture medium of the Ignacineria strain of the present invention actually promotes the root formation of plants, a cutting experiment was performed on pepper plants. Ignacineria strain culture medium cultured overnight (over-night) at OD600=1.0 in LB broth (10g/L tryptone, 5g/L yeast extract) from which salt has been removed after planting in topsoil, 2mL per pollen, at 3-day intervals processed. The untreated group was treated with tap water instead of the culture solution.

The red pepper plants rooted and established 2 weeks after cutting are shown in FIG. 5 . As a result of the experiment, it was confirmed that the rooting and rooting effect was remarkably excellent in the plants treated with the culture solution of the Ignacineria strain of the present invention.

Experimental Example 4: Root formation promoting effect on Koshihikari rice seedlings of Ignacineria strain culture medium

The effect of promoting root formation was confirmed by treating the culture solution of the Ignacineria strain of the present invention on the Koshihikari rice seedlings. The strain was cultured in LB broth without NaCl added, and centrifuged when the OD600 reached about 1.0 to settle the cells and recover the supernatant (sup.). The supernatant was prepared as a ¼ dilution, a ½ dilution, or a stock solution (1x), and 200 uL each was treated on an agar plate dented with rice seeds. The untreated group was treated with tap water instead of the culture solution. After about a week, the number of roots in each treatment group was confirmed (FIG. 6), and the average value of the number of roots was calculated (FIG. 7). From the above results, it was confirmed that the root formation of the Koshihikari rice seedlings was remarkably promoted when the Ignacineria strain culture solution of the present invention was treated.

Experimental Example 5: Root formation promoting effect on chrysanthemum plants of Ignacineria strain culture medium

A cutting experiment was performed on chrysanthemum plants using the culture medium of the Ignacineria strain of the present invention. After planting in the soil, the Ignacineria strain culture medium cultured overnight (over-night) at an OD600=1.0 in LB broth (10g/L tryptone, 5g/L yeast extract) from which salt was removed was treated twice a week at 1mL per pollen. did. The control was treated with tap water instead of the culture solution.

Fig. 8 shows chrysanthemum plants rooted and rooted 2 weeks after cutting. As a result of the experiment, it was confirmed that the number of irregular root formations was significantly increased in the chrysanthemum treated with the culture solution of the Ignacineria strain of the present invention.

Experimental Example 6: Early growth promoting effect of Ignacineria strain culture medium

The effect of promoting the initial growth of rice was tested using the culture medium of the Ignacineria strain of the present invention.

After soaking the rice seeds for about an hour, 7-8 grains were sown in pots with top soil. Ignacineria strain culture treated group was treated twice a week at 1mL per pollen, and untreated group (control group) was treated with tap water. The length of the longest leaf in each plant was measured from the 6th day to the 15th day of sowing, and the average of the treatment period is shown in FIG. 9 . As a result of the experiment, it was confirmed that the initial growth of rice was remarkably promoted when the culture solution of the Ignacineria strain of the present invention was treated.

Experimental Example 7: Effect of improving production of Ignacineria strain culture on red kale and basil plants

Growth and yield experiments were performed on red kale plants using the culture solution of the Ignacineria strain of the present invention. After transplanting into topsoil or topsoil to which chicken manure fertilizers have been added, incubate about OD600=1.0 in LB broth (10g/L tryptone, 5g/L yeast extract) from which salts have been removed and centrifuge the obtained Ignacineria strain culture filtrate. 0.5 to 1 mL per minute was treated twice a week. The control was treated with tap water instead of the culture solution.

In addition, a growth experiment was performed on basil plants using the culture solution of the Ignacineria strain of the present invention. After transplanting into the top soil, manure fertilizer, or coffee compost was added, the experiment was performed in the same manner as above.

The red kale plants grown 3 weeks after transplantation and the yield thereof are shown in FIG. 10 . The yield comparison graph disclosed in FIG. 10 is a cumulative graph of the yield compared three times at intervals of about 2-3 weeks. In addition, the basil plants grown 2 weeks after transplantation, and the yield thereof are shown in FIG. 11 .

As a result of the experiment, it was confirmed that the growth of red kale and basil plants treated with the Ignacineria strain culture solution of the present invention was significantly increased, thereby improving the production. In addition, when chicken manure manure or coffee compost was mixed, production was improved compared to when manure manure was used alone.

Korea Institute of Bioscience and Biotechnology KCTC14213BP 20200612

<110> COSMIC GREEN Inc. <120> Ignatzschineria strain producing auxin and promoting plant growth and uses thereof <130> P20-285 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 1404 <212> DNA <213> Artificial Sequence <220> <223> 16s rDNA of Ignatzschineria sp. CG 200001 <400> 1 catgcagtcg acggcagcac gaggaaagtt tactttcttc ggtggcgagt ggcggacggg 60 tgagtaatac ataggaatct accttttagt ctaggataac tacccgaaag ggtggctaat 120 actggatgtg gactacggtt taaagcaggg gaccttcggg ccttgcgcta agagatgagc 180 ctatgtggga ttagctagtt ggtgaggtaa tggctcacca aggcgacgat ctctagctgg 240 tttgagagga tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca 300 gcagtgggga atattggaca atgggcgaaa gcctgatcca gcaataccgc gtgtgtgaag 360 aaggccctag ggttgtaaag cacttttgtt agggagaacg ggtattatgg ttaataacca 420 tagtatctga tgttacctaa agaataagca ccggctaact ccgtgccagc agccgcggta 480 atacggaggg tgcaagcgtt aatcggaatt actgggcgta aagggcgcgt aggtggtatc 540 ttaagttggg tgtgaaagcc ccgggctcaa cctgggaatt gcatccaaaa ctggggtact 600 agagtgtggt agagggtagc ggaatttctg gtgtagcggt gaaatgcgta gatatcagaa 660 ggaacatcaa tggcgaaggc agctacctgg gccaacactg acgctgaggc gcgaaagcat 720 ggggagcaaa caggattaga taccctggta gtccatgcta taaacgatga caacttgttg 780 atggggggcac tggccttcgt tgacggagct aacgcattaa gttgtccgcc tggggagtac 840 ggtcgcaagg ctgaaactca aaggaattga cggggacccg cacaagcggt ggagcatgtg 900 gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatctacag aactttccag 960 agatggattg gtgccttcgg gaactgtaag acaggtgctg catggctgtc gtcagctcgt 1020 gtcgtgagat gttcggttaa gtccggcaac gagcgcaacc cttgtcctta tttgccagca 1080 cgtaatggtg ggaactctaa ggagactgcc ggtgataaac cggaggaagg tagggacgac 1140 gtcaagtcat catggccctt atgaccaggg ctacacacgt gctacaatgg tcggtacaat 1200 gggttgccaa gccgcgaggt ggagctaatc tcataaaacc gatcccagtc cggattgtag 1260 tctgcaactc gactacatga agtcggaatc gctagtaatc gtggatcaga atgccacggt 1320 gaatacgttc ccgggtcttg tacacaccgc ccgtcacacc atggaagttg attgctccag 1380 aagtaggtag ctaaaaatgg cgct 1404 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F forward primer <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 1492R reverse primer <400> 3 ggttaccttg ttacgacttc 20 <210> 4 <211> 1405 <212> DNA <213> Artificial Sequence <220> <223> 16s rDNA of Ignatzschineria sp. LJ11 <400> 4 catgcagtcg acggcagcac gaggaaagtt tactttcttc ggtggcgagt ggcggacggg 60 tgagtaatac ataggaatct accttttagt ctaggataac tacccgaaag ggtggctaat 120 actggatgtg gactacggtt taaagcaggg gaccttcggg ccttgcgcta agagatgagc 180 ctatgtggga ttagctagtt ggtgaggtaa tggctcacca aggcgacgat ctctagctgg 240 tttgagagga tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca 300 gcagtgggga atattggaca atgggcgaaa gcctgatcca gcaataccgc gtgtgtgaag 360 aaggccctag ggttgtaaag cacttttgtt agggagaacg ggtattacgg ttaataacca 420 tagtatctga tgttacctaa agaataagca ccggctaact ccgtgccagc agccgcggta 480 atacggaggg tgcaagcgtt aatcggaatt actgggcgta aagggcgcgt aggtggtatc 540 ttaagttggg tgtgaaagcc ccgggctcaa cctgggaatt gcatccaaaa ctggggtact 600 agagtgtggt agagggtagc ggaatttctg gtgtagcggt gaaatgcgta gatatcagaa 660 ggaacatcaa tggcgaaggc agctacctgg gccaacactg acgctgaggc gcgaaagcat 720 ggggagcaaa caggattaga taccctggta gtccatgcta taaacgatga caacttgttg 780 atggggggcac tggccttcgt tgacggagct aacgcattaa gttgtccgcc tggggagtac 840 ggtcgcaagg ctgaaactca aaggaattga cggggacccg cacaagcggt ggagcatgtg 900 gtttaattcg atgcaacgcg aagaacctta cctggtcttg acatctacag aactttccag 960 agatggattg gtgccttcgg gaactgtaag acaggtgctg catggctgtc gtcagctcgt 1020 gtcgtgagat gttcggttaa gtccggcaac gagcgcaacc cttgtcctta tttgccagca 1080 cgtaatggtg ggaactctaa ggagactgcc ggtgataaac cggaggaagg tagggacgac 1140 gtcaagtcat catggccctt atgaccaggg ctacacacgt gctacaatgg tcggtacaat 1200 gggttgccaa gccgcgaggt ggagctaatc tcataaaacc gatcccagtc cggattgtag 1260 tctgcaactc gactacatga agtcggaatc gctagtaatc gtggatcaga atgccacggt 1320 gaatacgttc ccgggtcttg tacacaccgc ccgtcacacc atggaagttg attgctccag 1380 aagtaggtag ctaaaaatgg gcgct 1405

Claims (8)

Ignatzneria ( Ignatzschineria ) genus CG200001 strain (KCTC14213BP). According to claim 1, wherein the 16S rDNA of the genus Ignacineria CG200001 strain is composed of the nucleotide sequence of SEQ ID NO: 1 Ignacineria CG200001 strain (KCTC14213BP). According to claim 1, wherein the genus Ignacineria CG200001 strain promotes the growth of plants Ignacineria genus CG200001 strain (KCTC14213BP). According to claim 1, wherein the Ignacineria genus CG200001 strain promotes the formation of roots of plants Ignacineria CG200001 strain (KCTC14213BP). According to claim 1, wherein the Ignacineria CG200001 strain is Ignacineria genus CG200001 strain (KCTC14213BP) to produce indole-3-acetic acid (Indole-3-acetic acid, IAA). The composition for promoting plant growth comprising the CG200001 strain of the genus Ignacineria of claim 1, the culture medium of the strain, or both as an active ingredient. Ignacineria genus CG200001 strain of claim 1, the culture solution of the strain, or agricultural microbial preparation comprising both of them as an active ingredient. The Ignacineria genus CG200001 strain of claim 1, a culture solution of the strain, a composition comprising the strain, and a composition comprising the culture solution of the strain, any one or more selected from the group consisting of a plant, a surrounding area of a plant, or both A method for promoting plant growth comprising the step of treating.

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