KR20220115498A - New Variety Bellflower Janghan - Google Patents

Abstract

The present invention provides Janghan as a new bellflower variety, which is crossbred between Yesan mountain bellflower, a wild species, and Andong medicinal bellflower, a native species, wherein varieties with a wide and flat head part, 5 to dozens of stems, and a lot of fine roots in proportion to the number of stems are selected. The Janghan bellflower according to the present invention is a bellflower developed for medicinal purposes with a patycodin D content more than four times higher than that of general bellflower to be very useful as health food or medicines, and is economically very advantageous by having many fine roots and weighing a lot.

Description

New Variety Bellflower Janghan

The present invention relates to a new variety of bellflower.

Bellflower (Platycodon grandiflorum (Jacq.) A. De Candolle) is a perennial herb that grows up to 20 years, and belongs to Campanulaceae (Mabberley 1987, The Plant Book. p. 461). So far, it has been reported that 600-950 species of about 35-55 genera are distributed worldwide (Kovanda 1978, Heywood VH. New York: Mayflower Books; pp254-256). It is known that only one species per genus exists in the genus Platycodon to which it belongs (Mabberley 1987, The Plant Book. p. 461).

Bellflower is a plant that grows mainly in China, Korea, and Japan, and its cultivation history is presumed to have been cultivated since the 16th century, from the record of dipping bellflower in the “Guhwangshuangyo (救荒撮要)” in the mid-16th century. There is almost no research on the origin of the bellflower, so little is known about it, and farmers have been cultivating it from generation to generation by collecting seeds from bellflower growing wild in the nearby hills.

The strains registered as bellflower varieties in Korea are Jangbaek Bellflower, Best White Bellflower, and KS Super Bellflower, which were secured through pure pedigree analysis of domestic bellflowers. In addition, although not registered, there are lines that have been cultivated from generation to generation by collecting seeds from white bellflower plants in Jeju Island, Miryang, and Asan.

1. Korean Patent Publication No. 10-2017-0133197

As described above, the development of new varieties of bellflower is not active, and its value is evaluated more highly, and development of a variety of bellflower with a high content of saponin, particularly Patycodin D, is urgently required.

In order to achieve the above object, the present invention provides a new variety of bellflower bellflower Janghan of accession number KACC 98120P established by crossing a wild species of Yesan sandflower as a parent and a native species of Andong Yakdoraji as a parent.

The new variety bellflower long-haired according to the present invention is crossed with a wild species, Yesan mountain bellflower, and a native species, Andong, wild bellflower, and the selection direction is wide and flat, and 5 or more to dozens of stems appear, and fine roots are also proportional to the stem. Thus, it was established by selecting and selecting a vigorous individual.

In the present invention, the new variety bellflower Janghan has the following characteristics.

1) Saponin content (Patycodin D-analysis result) is 4 times higher than normal bellflower,

2) The brain head is wide and large, and 5~10 or more stems appear from the 2nd year, and 10~30 or more stems occur in the 3rd year,

3) There are many fine roots in the roots, and on average, 3-year-old roots have 300g~1,000g.

In the present invention, the new variety bellflower Janghan has the following characteristics.

- The plant is slightly larger and the stem is thicker than that of the control variety, KS Super Doraja.

- The flower color of the KS Super Bellflower, which is the control variety, is purple compared to the blue one.

- The double color inside the petals, which is not found in the control variety KS Super Bellflower, is shown.

- The number of side branches of the above-ground stem is higher than that of the control cultivar, KS Super Bellflower, and the length between nodes is long.

- There are more side roots and thicker roots than KS Super Bellflower, which is the control variety.

The long-lived bellflower according to the present invention has a Patycodin D content that is four times higher than that of general bellflower, so it is very useful as a health food or medicine.

1 is a breeding system diagram according to the present invention.
2 and 3 are photographs of the appearance of long-term bellflower cultivation according to the present invention.
4 to 6DMS are graphs of Patycodin D content analysis results of long-grown bellflower and common bellflower according to the present invention (FIG. 4: 5-year-old bellflower, FIG. 5: 3-year-old bellflower, FIG. 36: 3-year-old general bellflower) .

Hereinafter, specific contents for carrying out the present invention will be described in detail.

1. Progression of upbringing

This bellflower cultivar "Janghan" is a cultivar that has been bred through efficacy verification as a medicinal bellflower unlike the main use of general bellflower for edible, cooking, or sweet fruit.

As for the background of the cultivation of this variety, Andong Yakdoraji, which is widely cultivated in Andong, the hometown of herbal medicine, had the disadvantage that it was difficult to cultivate it for more than 3 years, so breeding was started to improve it.

In 2010, the inventor discovered a bellflower similar to Andong Yakdoraji while cultivating Andong Yakdalaji in Sapgyo, Yesan-gun, Chungcheongnam-do, and searching for new resources in the surrounding mountains.

In fact, it was similar to Andong Yakdoraji, but the difference was the brain head. Andong Yakdalaji has a flat and wide head, a lot of stems, and many fine roots, but the collected bellflower has a long head, several stems, and many fine roots. However, it began to compensate for the shortcomings of Andong Yakdoraji, that it could not be raised to longevity and that it was weak against pests and diseases.

So, while crossbreeding between Yesan and Andong Yakdoraji collected in the mountains at that time, the selection direction was selected for individuals with a broad and flat head, 5 or more to dozens of stems, and strong fine roots in proportion to the stems until 2015. repeated. Since 2015, we have established cultivation methods such as direct sowing of seeds while harvesting in groups through individual propagation, and growing and transplanting seedlings using pots.

In addition, the functionality of the variety was entrusted to a specialized institution and tested. In 2019-2020, the cultivar was named Handoraji while conducting a characteristic test. The final cultivar name was “Janghan Bellflower”, and it was deposited with the National Academy of Agricultural Science (KACC), and the deposit number is KACC 98120P.

2. Nurturing Tree

A nurturing system diagram summarizing the above-described nurturing process is shown in FIG. 1 .

3, breed characteristics

○ Plant type: bellflower

○ Name of applied variety: Jang Han

○ Characteristic Investigator Name: Lee Bong-seon

2020.10.)○ Characteristic survey year: (2019.4.

2020.10.)

○ Characteristic survey location: 285, Ga-ri, Sapgyo-eup, Yesan-gun, Chungcheongnam-do, 374-15, Sapgyo-eup

○ Name of control cultivar (the most similar cultivar): KS Super Bellflower

No
characteristic
expression form
Application type
Control cultivar
One
2
3
4
5
6
7
8
9
No
actual value
No
actual value
One
Plant: grass
low
middle
high
40cm
21cm
2

Stem: Thick

thin
middle
thick

8mm
5mm
3
Stem: with or without coloring
none
have
9
One
4
Stem: colored part
bottom
central
Top
One
-
5
stem: color
light green
green
dark green
light brown
purple
Etc
One
One
6
Stem: side branches (dogs)
little
middle
many
18 pieces
11 pieces
7
stem: hair
none

have
One
One
8
Stem: Between nodes (mm)
short
middle
long
25mm
11mm
9
Node: Colored or not
none
have
One
One
10
Word: degree of coloration
little
middle
many
-
-
11
leaf: shape
wide line
long oval
oval
lanceolate
long elliptical epidermal lanceolate
ovoid
6
6

No
characteristic
expression form
Application type
Control cultivar
One
2
3
4
5
6
7
8
9
No
actual value
No
actual value
12

Leaf: tip shape

pointed Sharp Etc

One
One
13
Leaves: basal form
pointed round
vary
2
2
14
Blade: Length (mm)
short middle long 55mm
45mm
15
Blade: Width (mm)
narrow middle wide 40mm
33mm
16
Leaf: Wrinkle or not
none have One
One
17
Leaf: Density of edge teeth
thin middle thick 3
3
18
Leaves: color on the front
light green green
dark green Etc
3
2
19
Leaves: color on the back
light green green
dark green Etc
One
One
20
Flower: Shape
pocket type bell type
wide bell type remnant
T-shaped
3
3
21
flower: petal shape
single flower double flower One
One
22
Flowers: the direction in which they bloom
slightly upward
turned sideways
2
2
23
Flower: Length (mm)
short middle
long
43mm
23mm
24
Flower: Diameter (mm)
small
middle
Big
68mm
74mm

No
characteristic
expression form
Application type
Control cultivar
One
2
3
4
5
6
7
8
9
No
actual value
No
actual value
25
Flowers: color inside petals
White
light green
pink
purple
blue
Etc
4
6
blue purple
26
Flowers: the presence or absence of double color inside the petals
none
have
9
One
27
Flower: double-colored form inside the petals
speckled
stripe
Etc
2
-
28
Flower; double color of petals
White
light green
pink
purple
blue
Etc
6
light purple
-
29
Flower: Fragrance
none
have
One
One
30
Roots: Degree of occurrence of side roots
little
middle
many
7
3
31
Root: Thickness (mm)
thin
middle
thick
17mm
10mm
32
at flowering
fast
middle
late
3
3

4, photo data of the breed

[Applied variety] Bellflower long Han (field growth and plant body)

[Applied variety] Bellflower long Han (harvested root shape)

[Comparative variety] Bellflower KS Super (Package view)

[Control cultivar] Bellflower KS Super (plant and root)

[Applied variety] Bellflower long Han (flower shape and color)

[Comparative variety] Bellflower KS Super (shape and color of flowers)

5. Variety Characteristics Statement

1. Species name and scientific name: Bellflower ( Platycodon grandiflorum A .)
2. Varietal name: Janghan
3. Main Morphological Characteristics of Plants
- Janghan bellflower is a bellflower developed for medicinal use with a saponin content (Patycodin D-analysis result) that is 4 times higher than that of general bellflower.
- Long-term bellflower has a wide and large head, and 5~10 or more stems appear from the 2nd year, and 10~30 or more stems from the 3rd year.
- The root has many fine roots and weighs a lot. (Average 3-year root 300g~1,000g)
4. Characteristics that distinguish the applied cultivar from the reference cultivar
- Compared to the control variety, the plant height is slightly larger and the stem is thicker.
- The color of the flowers is blue for the control variety, but purple for the long bellflower.
- The long bellflower has a double color inside the petals, but the control variety does not have a double color.
- The number of side branches of the above-ground stem is more and the internode length is long.
- There are many side roots of the root and the thickness of the root is thick.
5. Describe the uniformity and stability of applied varieties (including control varieties)
- As a result of the annual survey, no mold strain was found, so there is uniformity and stability.
6. Additional information to help distinguish the applied variety
6.1 Insect resistance: Stronger disease and insect resistance than the control variety
6.2 Special Conditions for Variety Testing
- Propagation by seed and root propagation.
7. Information on breeding
7.1 Is the above variety bred using genetic modification (GMO)?
Yes ( ), No ( V )

6, Cultivation of long-lived bellflower

Long-term bellflower is mainly used for direct sowing, transplanting and special cultivation, and cultivation in sacks.

1) Direct sown cultivation (used for long-lived cultivation with more than 4 years of direct sown roots)

- Direct sowing is a customary cultivation method, and it is a method of direct sowing by making a wide ridge in the open field during November or March of the year and sprinkling seeds in rows.

- This is a method of deceiving and managing weeds while growing, harvesting a 1-year-old to 3-year-old root, transplanting it, and finally harvesting a 4-year to 5-year-old root.

- The characteristic of direct planting is not to grow the bellflower large, but to grow it small enough to be transplanted.

2) Transplantation (pot seedlings, 3-year-old root cultivation, harvest, 3-year-old root sacks)

- Unlike transplant cultivation, a nursery is established in early March of the year, sown in trays, grown for 50 to 60 days, and planted in the main field in early May.

- This is the main cultivation method of long-lived bellflower, and it is a cultivation method that can maximize the yield by cultivating 3-year-old bellflower for medicinal use.

- It can be seen that the outpost is distinctly different from that of general bellflower, and in particular, a lot of stems come up from the head of the brain.

- The tray sowing method has the advantage of forming a lot of fine roots while germinating and growing in a pot.

- In general bellflower, fine roots are not formed even using the tray sowing method.

3) Cultivation in sacks (ground cultivation = pot cultivation)

- This is a special cultivation method in which 1-year-old to 3-year-old or more long-term roots obtained through direct planting are transplanted into sacks and cultivated.

- If cultivated in sacks (pots) for more than 3 years, high-quality medicinal bellflower can be produced, and it is an organic method that can increase size, weight and strength.

7. Comparison of contents of Platycodin D component

-Experimental institution : Sungkyunkwan University Organic Analysis Lab.

- Experimental purpose : Comparative analysis of the content of Platycodin D in Janghan Bellflower and Normal Bellflower

-Experimental sample : 5 year old bellflower root, 3 year old bellflower root, 3 year old common bellflower root,

- Cultivation : Janghan bellflower is the cultivar for this application, and general bellflower is a native bellflower grown in Gari, Sapgyo-eup, Yesan-gun.

- Extraction method

All samples are dried and powdered prior to analysis.

Step 1. Add 0.3 g of sample to 15 mL of 70 % ethanol and mix well.

Step 2. Bath water treatment for 2 hours in a 50℃ constant temperature water bath (sonication is performed after bath treatment without a shaker)

Step 3. After 30 minutes of sonication, centrifuge at 4000 RPM for 15 minutes

Step 4. Repeat the equal amount recovery 2 times

Step 5. Concentrate treatment at 70℃ for 6-7 hours

Step 6. Dissolve in 2ml of water and analyze after filtering

- Inspection equipment and analysis conditions

Equipment name: LC-MS/MS

Column : ZORBAX 300SB-C18 5um, 4.6*250mm

Column temperature: 35 ℃

Injection volume : 10ul

Flow rate: 1ml/min

UV: 203 nm

Mobile phase: A-water, B-CAN

- Test result

The results are shown in Table 6 with reference to the test results of FIG. 4 (5-year-old long-grown bellflower), FIG. 5 (3-year-old long-grown bellflower), and FIG. 6 (3 year old general bellflower).

Platycodin-D
5 year old bellflower
3 year old bellflower
3 year old common bellflower
detection amount
493.9ng/ul
402.4 ng/ul
108.3 ng/ul

According to the results of Table 8, the content of Platycodin-D, which is a pharmacological component, of 5-year-old bellflower root of the present invention was about 5 times higher than that of general 3-year-old bellflower, and 3-year-old root was detected to be about 4 times higher. It can be seen that the higher the value, the more medicinal ingredients, and the pharmacological value is very excellent compared to general bellflower.

National Academy of Agricultural Sciences KACC98120P 20210913

Claims (1)

A new variety of bellflower Janghan with accession number KACC 98120P established by crossbreeding the wild species Yesan sandflower as the parent and the native Andong Yakdoraji as the parent.

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