KR20220112988A - A cosmetic composition for skin whitening containing loess extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, which is non-toxic and effective in improving skin whitening by containing ocher extract as an active ingredient.

Description

A cosmetic composition for skin whitening improvement containing loess extract as an active ingredient {A cosmetic composition for skin whitening containing loess extract}

The present invention relates to a cosmetic composition containing loess extract as an active ingredient.

As modern people are more interested in health, various studies on natural and eco-friendly cosmetics are being actively conducted. In functional cosmetics, interest in cosmetics using natural materials is increasing rather than in cases where chemical ingredients-based whitening, antioxidants, UV protection, preservatives, and antibacterial agents were used in the past.

Among various natural materials, animal-derived materials are actively developing functional cosmetics using plant-derived ingredients due to increased consumer rejection of animal-derived materials due to animal welfare, mad cow disease, swine cholera, and bird flu. Consumer demand for multifunctional cosmetics such as wrinkle improvement and UV protection is increasing.

Functional cosmetics refer to cosmetics in various stages of use, such as essence, serum, cream, powder, and base, with specific functions added such as whitening, wrinkle improvement, and UV protection. Cosmetics with one function have also been developed to increase the ease of use, and products specialized for various purposes such as pore care, nutrition supply, elasticity enhancement, acne treatment, whitening and UV protection are also being launched.

As it is difficult for new single substances to be imported into Korea and large cosmetic companies are concentrating on the development of multifunctional products for whitening and UV protection, whitening materials are rapidly emerging.

As whitening ingredients known to date, arbutin, kojic acid, azelaic acid, aloesin, 4-butylresorcinol, resveratrol, ceramide, sphingosine-1-phosphate, sphingosyl that inhibits tyrosinase enzyme activity Substances such as phosphorylcholine and the like, hydroquinone, and the like. However, there is a problem that some whitening ingredients have a limited amount of use due to safety issues such as irritation and redness when applied to the skin, or that practical effects cannot be expected due to insignificant effects. In particular, in the case of hydroquinone, its use was prohibited due to unwanted side effects such as irreversible whitening, and it has been replaced with arbutin, which is a structural analogue of hydroquinone. As such, in the field of whitening, it is particularly important to have a tyrosinase activity inhibitory effect and not only to have a whitening effect but also to secure stability.

The inventors of the present invention made diligent efforts to develop a material that can actually help skin whitening efficacy while securing safety when applied to the skin using natural materials as a material for cosmetics with skin whitening function. As a result, loess extract is The present invention was completed by confirming that it contains a high concentration of minerals and has an excellent whitening effect due to its high tyrosinase activity inhibition ability, and confirmed that it can be used for skin whitening purposes.

Republic of Korea Patent Publication No. 10-2019-0176556

An object of the present invention is to provide a cosmetic composition for improving skin whitening containing loess extract as an active ingredient.

In addition, another object of the present invention relates to a method capable of producing a large amount of loess extract.

In addition, another object of the present invention is to provide a health functional food composition or quasi-drug composition for improving skin whitening containing loess extract as an active ingredient.

In order to achieve the above object, the present invention can provide a cosmetic composition for skin whitening, characterized in that it contains loess extract as an active ingredient.

The loess extract may be a concentrate prepared by mixing loess with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, allowing it to stand for 1 to 500 days, recovering the supernatant, and distilling it under reduced pressure.

During the vacuum distillation, the distillation temperature may be 30 to 50° C., and the pressure may be 50 to 100 torr.

The loess may be obtained from a community where cypress trees grow wild.

When the loess extract was analyzed by ICP analysis, sodium 190.16 mg/l, magnesium 30.64 mg/l, aluminum (Al) 8.08 mg/l, iron 3.62 mg/l, calcium 65.3 mg/l and silica 82.04 mg/l contained may be doing

The loess extract may be to inhibit tyrosinase (tyrosinase).

In addition, in order to achieve the above other object, the method for preparing the cosmetic composition for skin whitening of the present invention may include the following steps.

(a) mixing ocher with a solvent; and

(b) distilling the mixture obtained from step (a) under reduced pressure under pressure of 30 to 50 °C and 50 to 100 torr to obtain a loess extract.

The solvent may be water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

It may further include; before step (b), (a1) filtering the mixed solution obtained from step (a).

The mixing weight ratio of loess and the solvent in step (a) may be 1:1 to 100.

Step (a) may be performed for 1 to 10 hours under a temperature condition of 10 to 40 °C.

In addition, in order to achieve the above another object, the health functional food composition for skin whitening of the present invention may contain the loess extract as an active ingredient.

In addition, in order to achieve the above another object, the quasi-drug composition for skin whitening of the present invention may contain the loess extract as an active ingredient.

The cosmetic composition containing the loess extract of the present invention as an active ingredient has a high inorganic substance content, excellent silica/alumina ratio among them, and excellent skin penetration, so it is suitable for use in various formulations.

In addition, the cosmetic composition containing the loess extract of the present invention as an active ingredient inhibits the synthesis of tyrosinase involved in the formation of melanin, thereby effectively inhibiting the production of melanin, thus skin whitening cosmetic composition has an excellent skin whitening effect.

1 is a photograph of a solution obtained by mixing ocher extract 1 of Example 1 in distilled water.
Figure 2 is a photograph of the solution after filtering the loess extract 1 of Example 1.
3 is a photograph taken of the loess extract 2 prepared in Example 2.
Figure 4 is a graph showing the XRD analysis results for the loess powder solution obtained from Comparative Example 1.
5 is a graph showing the XRD analysis results for the loess extract 1 prepared in Example 1.
6 is an image of the loess powder solution obtained from Comparative Example 1 observed by SEM-EDS.
7 is an image of observing loess extract 1 prepared in Example 1 by SEM-EDS.
FIG. 8 is a result confirming the DOPA-oxidase inhibitory efficacy for each concentration of loess extract 1 prepared in Example 1. FIG.
9 is a result confirming the DOPA-oxidase inhibitory efficacy for each concentration of loess extract 2 prepared in Example 2.
10 is a result confirming the dopa-oxidase inhibitory efficacy (DOPA-oxidase inhibitory efficacy) for each concentration of the kojic acid solution prepared in Comparative Example 2.

The present invention relates to a cosmetic composition, a health functional food composition, and a quasi-drug composition containing loess extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

Yellow soil is a natural soil consisting of diatomaceous earth and soil containing hydrous iron oxide and anhydrous iron oxide. It can be easily obtained all over Korea. In particular, yellow soil in Jeollanam-do contains a lot of iron oxide and has a strong red color. The yellow color is strong.

The loess extract called loess jijangsu is yellow soil water, also called mugeunsu. If you dig a pit more than 60 cm into the loess soil of a sunny field or deep ridge, soil like a green belt comes out, and the soil is dug out. It refers to a pale yellow water that is produced by mixing water filtered through charcoal and loess in an appropriate ratio, stirring it several times, and then after a certain amount of time has passed, impurities sink to the bottom. Red clay water contains the good ingredients of ocher, which purifies the blood, normalizes the hormone secretion of the body's endocrine glands, separates and discharges harmful ingredients to the human body, and maintains the acidified constitution as a weak alkaline body fluid, beautifies the skin and relieves constipation It is known to be effective. However, there are few studies on the whitening effect.

The cosmetic composition of the present invention contains a loess extract as an active ingredient, and the loess extract is an extract obtained by mixing loess with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and then standing for 1 to 500 days; Or it may be a fraction obtained by fractionating the extract, or a concentrate prepared by distilling the extract under reduced pressure. The meaning of the extract also includes a solid extract obtained by methods such as freeze drying, vacuum drying, hot air drying, spray drying, and the like.

The present invention measured the tyrosinase inhibitory activity that causes pigmentation due to imbalance in the melanin formation process to evaluate the skin whitening effect of the loess extract, and as a result, the loess extract is superior to tyrosinase ( tyrosinase) inhibitory effect, confirming that the skin whitening effect is excellent. In addition, it was confirmed that the concentrate prepared by distilling the extract under reduced pressure among the loess extracts was superior to the tyrosinase inhibitory effect by 2 to 6 times or more than the Jijang water obtained by simple extraction of loess. In addition, as a result of measuring cytotoxicity, it was confirmed that there was no cytotoxicity unlike kojic acid.

The loess extract of the present invention is an extract obtained by mixing loess with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and then standing or leaching for 1 to 500 days, or a fraction obtained by fractionating the extract, or It may be a concentrate prepared by distilling the extract under reduced pressure, preferably by mixing loess with a solvent and allowing it to stand for 1 to 500 days, and then distilling the supernatant obtained under reduced pressure, more preferably mixing loess with a solvent. As a concentrate obtained by distilling under reduced pressure, and recovering only the supernatant except for the solids that have been settled by allowing it to stand for 1 to 500 days, specifically, loess and the solvent are 1:1 to 100, preferably 1: 5 to 20, more preferably Preferably, it is mixed in a weight ratio of 1: 10 to a distillation temperature of 30 to 50 °C, preferably 40 to 50 °C, and a distillation pressure of 50 to 100 torr, preferably 60 to 90 torr, more preferably 70 to 85 torr. It may be a concentrate prepared by distillation under reduced pressure.

The mixture of the loess and the solvent or the supernatant obtained therefrom is filtered before distillation under reduced pressure; may further include, through which loess fine particles and impurities can be removed. For the filtration process, sand filtration, a filtration method using a micro filter, and a filtration method using an ultra filter may be used. The mixed solution is sent to a vacuum distillation device.

If the extract is not obtained from loess, a small amount of the active ingredient is extracted and the skin whitening effect cannot be achieved, and when the vacuum distillation step is omitted, the skin whitening effect is low.

When the weight ratio of the loess to the solvent is out of the above range, the active ingredient of loess may be present in a small amount in the extract.

In addition, when the temperature and pressure conditions of the reduced pressure distillation are less than the lower limit, the active ingredient may be extracted in a small amount from the loess, and if it exceeds the upper limit, the active ingredient is also distilled to reduce the effect or an unwanted effect may be added.

In addition, before the vacuum distillation step, the step of mixing the loess with the solvent is 10 to 40 ℃, preferably 20 to 30 ℃ under temperature conditions, 1 to 500 days, preferably 150 to 450 days, more preferably It can be carried out for 410 to 420 days, and when it is less than the lower limit, the active ingredient of loess can be extracted in a small amount, and when it exceeds the upper limit, the ingredients that are not the active ingredient or modified by heat are extracted, and the functionality can be reduced. have.

The solvent may be water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. As the lower alcohol, 20 to 99% by volume of an aqueous solution of methanol, ethanol, butanol or propanol may be used, and preferably water may be used for excellent whitening effect and non-toxicity.

The loess used in the present invention may be obtained from a community where cypress trees grow naturally, and it is preferable to use that obtained from the Galmobong cypress forest located in Idang-ri, Goseong-eup, Goseong-gun. If loess obtained from ordinary soil is used, the active ingredient of loess can be extracted in a small amount, and the functionality may be reduced due to impurities.

When the loess extract was analyzed by ICP analysis, sodium 190.16 mg/l, magnesium 30.64 mg/l, aluminum (Al) 8.08 mg/l, iron 3.62 mg/l, calcium 65.3 mg/l and silica 82.04 mg/l contained As a result, it contains various mineral components in high concentrations and has the effect of significantly and remarkably improving whitening activity.

Although the loess extract did not contain flavonoids or polyphenol components, it had a high tyrosinase activity inhibitory effect, and the tyrosinase activity inhibitory effect increased in a concentration-dependent manner, confirming that it had a whitening effect.

The loess extract of the present invention may be used in health functional food and quasi-drug compositions in addition to cosmetic compositions.

The present invention comprises the steps of (a) mixing loess with a solvent; and (b) distilling the mixture obtained from step (a) under reduced pressure under pressure of 30 to 50° C. and 50 to 100 torr to obtain a loess extract; provides a method for preparing a cosmetic composition for skin whitening comprising.

The loess cypress used in the present invention may be obtained from a community where it grows naturally, and it is preferable to use one obtained from the cypress forest of Galmobong located in Idang-ri, Goseong-eup, Goseong-gun. If loess obtained from ordinary soil is used, the active ingredient of loess can be extracted in a small amount, and the functionality may be reduced due to impurities.

The loess used in the present invention is preferably used by securing and using 1 m of loess from the surface layer in consideration of soil contamination. It can be subjected to high heat treatment at 1000 °C or higher for

The solvent may be water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. As the lower alcohol, 20 to 99% by volume of an aqueous solution of methanol, ethanol, butanol or propanol may be used, and preferably water may be used for excellent whitening effect and non-toxicity.

In the manufacturing method, in step (a), the loess and the solvent may be mixed in a weight ratio of 1:1 to 100, preferably 1:5 to 20, more preferably 1:10. When the mixing weight ratio of the loess and the solvent is out of the above range, the active ingredient of the loess may be extracted in a small amount in the extract.

Step (a) may be carried out for 1 to 500 days, preferably 150 to 450 days, more preferably 410 to 420 days under a temperature condition of 10 to 40 ℃, preferably 20 to 30 ℃, If it is less than the lower limit, the active ingredient of loess may be extracted in a small amount, and if it exceeds the upper limit, the ingredients that are not the active ingredient or modified by heat are extracted, and the functionality may be rather reduced.

It may further include; before step (b), (a1) filtering the mixed solution obtained from step (a), through which it is possible to remove loess fine particles and impurities present in the mixed solution.

For the filtration process, sand filtration, a filtration method using a micro filter, and a filtration method using an ultra filter may be used. The mixed solution is sent to a vacuum distillation device.

The step (b) is concentrated by distillation under reduced pressure at a distillation temperature of 30 to 50 °C, preferably 40 to 50 °C, and a distillation pressure of 50 to 100 torr, preferably 60 to 90 torr, more preferably 70 to 85 torr. it may have been

In the case of distillation under reduced pressure without going through the leaching process for extracting the active ingredient from the loess, a sufficient skin whitening effect cannot be expected because the active ingredient is extracted in a small amount. In addition, if only the leaching process using loess is performed and the vacuum distillation step is omitted, a skin whitening effect can be obtained, but since the effect is less than half that of conventional whitening materials, steps (a) and (b) are It is best to do all of them.

In addition, when the temperature and pressure conditions of the reduced pressure distillation are less than the lower limit, a small amount of the active ingredient may remain from the loess, and if it exceeds the upper limit, the effect is reduced or undesirable because a large amount of other substances are also distilled and removed in addition to the active ingredient. Non-existent effects may be added.

The term 'extract' used while referring to loess in this specification includes not only the crude extract obtained by treating the extraction solvent, but also the processed product such as a concentrate obtained by distilling the loess extract under reduced pressure. For example, the loess extract may be prepared in a powder state by an additional process such as freeze drying or spray drying. Preferably, the loess extract may be a concentrate obtained by distilling a solution obtained by treating the extraction solvent under reduced pressure.

In the cosmetic composition according to the present invention, the cosmetic composition for skin whitening may contain 0.0001 to 90 parts by weight of the loess extract based on the total weight, but is not limited thereto, as long as it contains an effective amount capable of providing a desired whitening improvement effect. .

As used herein, the term 'skin whitening or whitening' refers to suppressing, inhibiting or alleviating hyperpigmentation of the skin. Hyperpigmentation of the skin includes freckles, melasma, hyperpigmentation after UV exposure, hyperpigmentation after inflammation, age spots, brown spots or age spots, and the like.

Meanwhile, in the present specification, the term 'contained as an active ingredient' means including an effective amount sufficient to achieve the efficacy or activity of the loess extract. For example, the loess extract is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. Since the loess extract as a natural product does not have any side effects on the human body even when used in excess, the upper limit of the quantity of the loess extract included in the composition of the present invention can be selected and implemented by those skilled in the art within an appropriate range.

In the cosmetic composition of the present invention, in addition to the above cosmetic composition, other ingredients commonly formulated in cosmetics may be blended as needed. Examples of such blending ingredients include oil and fat ingredients, humectants, emollients, surfactants, organic and inorganic pigments, Organic powder, UV absorber, preservative, disinfectant, antioxidant, pH adjuster, alcohol, colorant, fragrance, blood circulation promoter, cooling agent, limiting agent, purified water, water-soluble vitamin, fat-soluble vitamin, polymer peptide, polymer polysaccharide, sphingolipid and seaweed an extract, etc. are mentioned.

The cosmetic composition of the present invention may be prepared in the form of emulsified formulations and solubilized formulations commonly used in the art.

In addition, the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the above components as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural fragrances; It may further include a carrier.

Products to which the composition of the present invention can be added include, for example, mist, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, sunscreen cream , moisture cream, hand cream, foundation, essence, nourishing essence, mask pack, press powder, loose powder, eye shadow, etc., and soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

In addition, the present invention provides a health functional food composition containing loess extract as an active ingredient.

Health functional food refers to food prepared by adding loess extract to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspension, etc. However, unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long period of time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The amount of loess extract added in such a health functional food varies depending on the type of health functional food and cannot be uniformly defined, but it can be added within a range that does not impair the original taste of the food, and is usually 0.01 for the target food. to 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

In addition, the present invention provides a quasi-drug composition containing loess extract as an active ingredient.

In the present invention, the term 'quasi-drug' refers to articles with a milder action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals. Quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

The quasi-drug composition may be used in combination with other quasi-drugs or quasi-drug ingredients by adding loess extract as it is, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use.

The quasi-drug composition of the present invention is not limited thereto, but preferably a body cleanser, disinfectant cleaner, cleaner, kitchen cleaner, cleaning agent, toothpaste, gargle, wet tissue, detergent, soap, hand wash, hair cleaner, hair softener, humidifier filler , may be one or more formulations selected from the group consisting of masks, ointments and filter fillers. The same contents as described for the cosmetic composition may be applied to the quasi-drug composition of the present invention, except for differences depending on the formulation.

The present invention provides a method for improving skin whitening comprising administering the loess extract to an individual.

As used herein, the term “individual” refers to all animals, including mammals, including mice, livestock, and humans.

In the skin whitening improvement method of the present invention, the dosage, administration route, administration method, etc. of the ocher distillation composition may be appropriately selected.

Specifically, 'administration' in the present invention means introducing the composition of the present invention to an individual by any suitable method, and the route of administration of the composition is through any general route as long as it can reach the target tissue and exhibit a skin whitening effect. may be administered. The administration may include application to the skin, and may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, oral administration, topical administration, or intranasal administration, but is not limited thereto. Specifically, since the composition of the present invention has a skin whitening effect, the administration route of the composition may be administered by applying to the skin.

The composition of the present invention may be administered daily or intermittently, and the number of administrations per day may be administered once or divided into two to three times. In addition, the composition of the present invention may be used alone or in combination with other substances for skin whitening. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1. Loess Extract 1

Loess soil was obtained from the cypress forest located in Goseong-gun, and it was used after refrigeration. The cypress forest-derived loess and distilled water were mixed in a weight ratio of 2: 20 and left at room temperature (25 ° C.) for 416 days (10000 hours), then only the supernatant was recovered, excluding the solids formed by sedimentation, and filtered After removing the fine solids, only the filtrate was recovered to obtain 20 ml of loess extract. The pictures before and after filtration are shown in FIGS. 1 and 2 .

Example 2. Loess Extract 2

Loess soil was obtained from the cypress forest located in Goseong-gun, and it was used after refrigeration. The cypress forest-derived loess and distilled water were mixed in a weight ratio of 2: 20 and left at room temperature (25 ° C.) for 416 days (10000 hours), then only the supernatant was recovered, excluding the solids formed by sedimentation, and filtered After removing the fine solids, only the filtrate was recovered. The filtrate (about 20 ml) was distilled under reduced pressure at 45 ° C. at 79 torr, and at this time, by recovering the concentrated residual liquid, loess extract (or 'loess concentrate' or 'loess extract concentrate') 1 ml was obtained (FIG. 3).

Comparative Example 1. Loess powder

2 mg of loess powder obtained from the cypress forest located in Goseong-gun was prepared. In the experiment, 1 mg of loess powder was analyzed using a solution mixed with 10 ml of distilled water.

Comparative Example 2. Kojic acid solution

Kojic acid (5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one) (CAS:501-30-4) was purchased from Sigma and used. The kojic acid was diluted by concentration (0, 0.025, 0.05, 0.075, 0.1, 1 mg/ml) using distilled water to prepare a kojic acid solution.

<Test Example>

Test Example 1. Component Analysis of Hwangchi Extract

1) pH analysis

The loess extract of Examples 1 and 2 and the cypress extract of Comparative Example 3 were prepared, and the pH of each solution was measured immediately using pH paper. As a result, it was confirmed that the loess extracts of Examples 1 and 2 had a pH of 4.5-5.

2) XRD analysis

To analyze the components of the loess extract 1 of Example 1 and the loess powder solution of Comparative Example 1, the analysis was performed using an X-ray diffractometer (XRD, D8 Advance, Bruker, Germany).

4 is a graph showing the XRD analysis result of the loess powder solution obtained from Comparative Example 1, and FIG. 5 is a graph showing the XRD analysis result of the loess extract 1 prepared in Example 1.

As shown in FIGS. 4 and 5 , in the case of the loess powder, the main component was silica (SiO ₂ ), but it was confirmed that the loess extract contained a large amount of minerals in addition to silica.

3) SEM-EDS analysis

To analyze the chemical composition of the loess extract 1 of Example 1 and the loess powder solution of Comparative Example 1, SEM-EDS (Scanning Electron Microscopy with Energy Dispersive Spectroscopy) (Hitachi, S-4800, Japan) was used. At this time, in the case of loess powder, 1 mg of loess powder was mixed with 10 ml of distilled water and then analyzed.

6 is an image of the loess powder solution obtained from Comparative Example 1 observed by SEM-EDS, and FIG. 7 is an image of the loess extract 1 prepared in Example 1 observed by SEM-EDS.

As shown in FIGS. 6 and 7 , it was confirmed that a large amount of components were mixed in the loess powder, specifically silica (SiO ₂ ), alumina (Al ₂ O ₃ ), organic (carbon, C), inorganic (Cl). , K, Fe, etc.) were confirmed to exist.

On the other hand, it was confirmed that the loess extract was more diverse than the loess powder and contained a high concentration of inorganic substances, specifically silica (SiO ₂ ), alumina (Al ₂ O ₃ ), organic (carbon, C), inorganic (Cl). , K, Fe, Ca, Na, Mg, etc.) were confirmed to be present. In particular, it can be seen that the loess extract significantly increased the content and types of minerals compared to the loess powder.

In addition, through the high content of oxygen (O) component of the loess concentrate, it can be seen that the inorganic substances exist in an oxidized form. For example, it may be calcium carbonate, magnesium oxide, alumina, silica, iron oxide (Fe ₂ O ₃ ) and the like.

4) ICP analysis

The components and contents of the loess extract 2 prepared in Example 2 and the loess powder solution of Comparative Example 1 were quantitatively analyzed with an inductively coupled plasma spectrophotometer (ICP-OES, Perkin Elmer, Waltham, MA, USA) to Table 1 shown in

division Composition of ions (mg/L) Na Mg Al Fe Ca Si Example 2 190.16 3.064 8.08 3.62 65.3 82.04 Comparative Example 1 0.076 0.005 0.026 0.006 0.015 0.012

According to the ICP analysis shown in Table 1, the loess extract 2 prepared in Example 2 had a Si/Al elemental composition ratio of about 10.15. On the other hand, the loess powder solution of Comparative Example 1 has a Si/Al element composition ratio of about 0.46. That is, although the loess extract 2 prepared in Example 2 was a concentrated solution of distilling loess, it was confirmed that not only had a higher silica/alumina ratio but also minerals were present in a high concentration. In the case of silica, it is a representative additive that improves the spreadability and spreadability of cosmetics on the skin, and inorganic substances such as K, Mg, and Fe (also referred to as 'minerals') play a very important role in improving biometabolism and skin penetration. It can be seen that the loess extract is a cosmetic, and it is easy to use regardless of the formulation.

Test Example 2. Analysis of whitening activity of loess extract and loess Jiangsu

1) DOPA oxidation inhibition assay

To analyze the whitening activity, the inhibitory activity of tyrosinase was confirmed by inhibiting DOPA oxidative activity, which was tested by modifying Flurkey's method. The DOPA oxidative activity inhibition assay was performed by measuring the DOPA quinone production using L-DOPA. For the sample, the loess extract 1 prepared in Example 1 and the loess extract 2 prepared in Example 2 were used, and the sample was dissolved in distilled water and diluted to 5 concentrations of 0%, 25%, 50%, 75%, and 100%. was used. As a positive control, the kojic acid solution of Comparative Example 2 was used, which was also used at 5 concentrations of 0 mg/ml, 0.025 mg/ml, 0.05 mg/ml, 0.075 mg/ml, 0.1 mg/ml, and 1 mg/ml. It was diluted and used.

Acontrol: 0.2 mL of 10 mM L-3,4-dohydroxyphenylalanine in 0.6 mL of 100 mM sodium phosphate buffer adjusted to pH 6.8, mushroom tyrosinase (100 U/mL) 0.2 mL was added and reacted at 37 °C for 5 minutes.

Bcontrol: 0.2 mL of 10 mM L-DOPA (L-3,4-dohydroxyphenylalanine) was added to 0.8 mL of 100 mM sodium phosphate buffer adjusted to pH 6.8, followed by reaction at 37° C. for 5 minutes.

Asample: 0.2 mL of 10 mM L-3,4-dohydroxyphenylalanine in 0.5 mL of 100 mM sodium phosphate buffer adjusted to pH 6.8, mushroom tyrosinase (100 U/mL) 0.2 mL and 0.1 mL of the sample were added and reacted at 37 °C for 5 minutes.

Bsample: 0.2 mL of 10 mM L-DOPA (L-3,4-dohydroxyphenylalanine) and 0.1 mL of sample were added to 0.7 mL of 100 mM sodium phosphate buffer adjusted to pH 6.8, followed by reaction at 37° C. for 5 minutes.

Then, the absorbance of each of the solutions was measured at a wavelength of 475 nm using a spectrophotometer, and the DOPA oxidation activity inhibition ability was calculated using Equation 1 below.

[Equation 1]

DOPA oxidation inhibitor efficacy (%) = [1 - (Asample-Bsample/Acontrol-Bcontrol)]ㅧ100

8 is the result of confirming the dopa-oxidase inhibitory efficacy by concentration for the loess extract 1 prepared in Example 1, and FIG. 9 is the dopa by concentration for the loess extract 2 prepared in Example 2 It is the result of confirming the oxidation inhibitory effect (DOPA-oxidase inhibitory efficacy), and FIG. 10 is the result of confirming the dopa oxidation inhibitory effect (DOPA-oxidase inhibitory efficacy) for each concentration of the kojic acid solution prepared in Comparative Example 2, three times Independent experimental results are expressed as mean ㅁ standard deviation.

8 to 10, it was confirmed that both the loess extract 1 of Example 2 and the loess extract 2 of Example 1 had a tyrosinase inhibitory rate. In particular, the loess extract 2 prepared from Example 2 of the present invention had a tyrosinase inhibition rate at least 2 times higher than that of the loess extract 1 of Example 1, and was confirmed to be at least 6 times higher at the highest concentration of 100%. , It can be seen that the loess extract 2 prepared from Example 2 of the present invention has a significantly higher dopa oxidation inhibitory effect than the general loess Jiangsu.

The loess extract 1 of Example 1 showed only a maximum of 20% tyrosinase inhibition, and it was confirmed that the tyrosinase inhibition rate was rather reduced at a concentration of 50% or more. 2 increased the activity in a concentration-dependent manner. That is, it can be seen that the loess extract 2 prepared in Example 2 of the present invention has a difference in effect from that of a component that is more than simply concentrated loess Jiangsu (loess extract).

It was confirmed that the loess extract 2 prepared in Example 2 exhibited a similar level of activity to the kojic acid solution as a positive control.

Test Example 3. Measurement of cell viability by MTT assay

In order to evaluate the effect of loess extract 2 prepared in Example 2 on cytotoxicity, MTT assay was used. MTT assay may be referred to as a standard colorimetric assay as a laboratory test method for measuring cell viability. MTT assay, a technique that can accurately measure cell proliferation and living cells, is one of the essential techniques in the field of life sciences, especially in tumor biology.

In order to confirm the toxicity of the loess extract 2 prepared in Example 2 of the present invention, an experiment was performed with human dermal fibroblasts (HDFa). First, human fibroblasts (Human Dermal fibroblast: HDFa) were mixed with Medium 106 with Low Serum Growth Supplement, and in a medium containing penicillin (100IU/ml) and streptomycin (100μg/ml) at 37°C, 5% CO ₂ It was cultured in the containing incubator. The cells were aliquoted in a 96 well plate and then cultured for 18 hours in cell culture conditions. After removing the medium and washing with PBS, a new medium and a sample were added, respectively, and incubated for 18 hours. After 18 hours, in order to measure the viability of the cells, the mitochondria activity of the living cells was measured to confirm the cell viability by performing the MTT ASSAY to check the viability of the cells, and the results are shown in Table 2 below.

division ocher extract of Example 2, concentration mg/ml 0 0.1 One 10 50 100 Cell viability (%) 100 110.2 102.5 105.4 111.4 113.5

As shown in Table 2, the loess extract 2 prepared from Example 2 of the present invention showed a high cell viability of 100% or more in all samples for fibroblasts.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Preparation Example 1. Preparation of granules

1,000 mg of loess extract of Example 2

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Preparation Example 2. Preparation of functional beverage

1,000 mg of loess extract of Example 2

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to a conventional health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, and then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and national preferences such as demand class, demand country, and use purpose.

A preparation example of a cosmetic composition suitable for applying the present invention will be presented.

Preparation example 3: lotion

Preparation examples of the lotion among the cosmetics containing the loess extract of Example 2 are shown in Table 3 below.

ingredient Content (wt%) ocher extract of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PG 1500 1.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 4: Lotion

Preparation examples of the lotion in the cosmetic containing the loess extract of Example 2 are shown in Table 4 below.

ingredient Content (wt%) ocher extract of Example 2 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 tocopheryl acetate 3.0 liquid paraffin 5.0 squalane 3.0 Macadamina Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation 5: Nourishing Cream

Examples of the preparation of the nutritional cream in the cosmetic containing the loess extract of Example 2 are shown in Table 5 below.

ingredient Content (wt%) ocher extract of Example 2 1.0 lipophilic monostearate glycerin 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearylisostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation 6: Essence

Preparation examples of the essence in the cosmetics containing the loess extract of Example 2 are shown in Table 6 below.

ingredient Content (wt%) ocher extract of Example 2 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate 8.0 Carboxyvinyl Polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 7: Emulsion for mask pack

Preparation examples of the emulsion for a mask pack among the cosmetics containing the loess extract of Example 2 are shown in Table 7 below.

ingredient Content (wt%) ocher extract of Example 2 1.0 polyvinyl alcohol 15.0 Cellulose Gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Claims (13)

A cosmetic composition for skin whitening, characterized in that it contains loess extract as an active ingredient. According to claim 1,
The loess extract is prepared by mixing loess with water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof and allowing it to stand for 1 to 500 days, then recovering the supernatant and distilling it under reduced pressure. A cosmetic composition for skin whitening. 3. The method of claim 2,
The cosmetic composition for skin whitening, characterized in that the distillation temperature during the vacuum distillation is 30 to 50 ℃, and the pressure is 50 to 100 torr. 3. The method of claim 2,
The ocher is a cosmetic composition for skin whitening, characterized in that it is obtained from a colony of cypress trees. According to claim 1,
When the ocher extract was analyzed by ICP analysis, it contained 190.16 mg/l of sodium, 30.64 mg/l of magnesium, 8.08 mg/l of aluminum (Al), 3.62 mg/l of iron, 65.3 mg/l of calcium and 82.04 mg/l of silica. A cosmetic composition for skin whitening, characterized in that According to claim 1,
The ocher extract is a cosmetic composition for skin whitening, characterized in that it inhibits tyrosinase. (a) mixing ocher with a solvent; and
(b) distilling the mixture obtained from step (a) under reduced pressure under pressure of 30 to 50 ° C and 50 to 100 torr to obtain a loess extract; manufacturing method. 8. The method of claim 7,
The solvent is water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a method for producing a cosmetic composition for skin whitening, characterized in that a mixed solvent thereof. 8. The method of claim 7,
Before step (b), (a1) filtering the mixture obtained from step (a); Method for producing a cosmetic composition for skin whitening, characterized in that it further comprises. 8. The method of claim 7,
The method of manufacturing a cosmetic composition for skin whitening, characterized in that the mixing weight ratio of loess and the solvent in step (a) is 1:1 to 100. 8. The method of claim 7,
Step (a) is a method of manufacturing a cosmetic composition for skin whitening, characterized in that it is performed for 1 to 10 hours under a temperature condition of 10 to 40 ℃. A health functional food composition for skin whitening, characterized in that it contains loess extract as an active ingredient. A quasi-drug composition for skin whitening, characterized in that it contains loess extract as an active ingredient.

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