KR20220110360A - Food composition for pets containing the extract of Sasa borealis and its manufacturing method - Google Patents

Abstract

The present invention provides a food composition for companion animals containing an extract of Sasa borealis. By using the food composition for companion animals containing the extract of Sasa borealis according to an embodiment of the present invention, it is possible to provide the food composition, which can suppress the obesity of the companion animals and is optimal while being preferred by the companion animals.

Description

Food composition for pets containing the extract of Sasa borealis and its manufacturing method

The present invention relates to a food composition for companion animals comprising a bamboo extract and a method for preparing the same.

The size of the companion animal-related market has doubled from 900 billion won in 2012 to 1.8 trillion won in 2015, surpassed 3 trillion won in 2018, and is expected to expand to 5.8 trillion won in 2020. .

As the size of the companion animal market increases, food for companion animals, supplies, and even housing are being distributed.

In particular, as the status of companion animals has been upgraded from 'pet' to 'companion', companion animal beverages need to be provided to improve the nutrition and condition of companion animals beyond simply providing them to quench their thirst and supply moisture. have.

As a prior art related to companion animals, there is registered Patent No. 10-1855663 (May 01, 2018) (hereinafter referred to as prior art), which uses readily available food raw materials to digest and absorb companion animals. Provides a homemade feed composition for companion animals and a method of manufacturing the same that can promote long-term intake, improve bowel movements, reduce ALP levels, and are nutritionally excellent.

However, prior art focusing on the health of companion animals, particularly the efficacy of suppressing obesity in companion animals, is nonexistent or insufficient.

Republic of Korea Patent Publication No. 10-1856297 (2018.05.09. Announcement)

The problem to be solved by the present invention is to provide a food composition for companion animals including a bamboo extract ( Sasa borealis ).

The present invention provides a food composition for companion animals comprising a bamboo extract ( Sasa borealis ).

Compared to 100% by weight of the total composition, it may contain 0.5 to 4% by weight of the bamboo extract.

The composition may have an anti-obesity effect.

The composition may further include a beef extract and a pear extract.

Based on 100% by weight of the total composition, 2 to 4% by weight of the beef extract and 0.1 to 1% by weight of the pear extract may be included.

The composition may further include calcium pantothenate, vitamin B2, vitamin C and fructooligosaccharide.

The food may be a beverage.

According to another embodiment of the present invention, the method for producing a food composition for companion animals comprising a bamboo extract ( Sasa borealis ) comprises the steps of: (a) preparing a bamboo extract, a beef extract and a pear extract, (b) the bamboo extract, Mixing the beef extract and the pear extract to prepare a mixture and (c) sterilizing the mixture.

Each extract in step (a) may be extracted with hot water at a temperature of 90 to 100 ℃.

In step (b), 0.5 to 2% by weight of the bamboo extract, 2 to 4% by weight of the beef extract, and 0.1 to 1% by weight of the pear extract may be mixed with respect to 100% by weight of the total composition.

Step (c) may be performed at a temperature of 90 to 100° C. for 20 to 40 minutes.

By using the food composition for companion animals including the bamboo extract according to an embodiment of the present invention, it is possible to suppress obesity in companion animals, as well as provide an optimal food composition preferred by companion animals.

1 shows a method for preparing a food composition for companion animals including a bamboo extract according to an embodiment of the present invention.
Figure 2 is an evaluation of the cell viability of adipocytes, muscle cells, and hepatocytes for the extract of bamboo shoots.
Figure 3 shows the activation results of AMPK in adipocytes and skeletal muscle cells of the extract of Zordi.
Figure 4 shows the activation result of AMPK in hepatocytes induced by localization of the extract of S. japonica.
Figure 5 shows the change in the body weight of the mice fed the extract of the bamboo grass.
Figure 6 shows the effect of improving the abdominal fat of the mice fed with the extract of the bamboo grass.
7 shows an image, GPT, and GOT analyzing the effect of improving fatty liver in mice fed the extract of Zordi.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings. First, it should be noted that in the drawings, the same components or parts are denoted by the same reference numerals as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted so as not to obscure the gist of the present invention.

As used herein, the terms about, substantially, etc., are used in a sense at or close to the numerical value when manufacturing and material tolerances inherent in the stated meaning are presented, and are intended to be accurate or to aid in the understanding of the present invention. Absolute figures are used to prevent unreasonable exploitation of the stated disclosure by unscrupulous infringers.

The present invention provides a food composition for companion animals comprising a bamboo extract (Sasa borealis) , and the composition has an effect of suppressing obesity in companion animals.

The food composition for companion animals including the bamboo extract (Sasa borealis) according to an embodiment of the present invention may include 0.5 to 4% by weight of the bamboo extract relative to 100% by weight of the total composition.

If the content of the bamboo extract is less than 0.5% by weight, the effect of suppressing the obesity of companion animals is insignificant, and when it exceeds 4% by weight, there is a problem that the palatability is lowered and the companion animal cannot consume it.

In the present invention, reeds are several perennial plants of the Grapeaceae family, and are plants that grow in groups in the central and southern regions of Korea and in the mountains of Jeondo Island.

In addition, it is a medicinal herb with excellent medicinal properties that surpasses ginseng. It is the strongest among bamboo and is known to be effective in intractable diseases such as diabetes, gastritis, gastric ulcer, chronic hepatitis, and cancer. , It is effective in removing phlegm and excreting urine, smoothing the metabolism, and suppressing cancer cells that treat inflammation.

Reeds having such an effect can use the concentrated extract after hot water extraction in the present invention.

The food composition for companion animals including a bamboo extract according to an embodiment of the present invention may contain 2 to 4% by weight of the beef extract and 0.1 to 1% by weight of the pear extract relative to 100% by weight of the total composition.

If the content of the pear extract is less than 0.1% by weight, there is a problem that companion animals do not consume it due to reduced palatability, and even if it exceeds 1% by weight, palatability is lowered, which is not economically desirable.

The food composition for companion animals including the bamboo extract according to an embodiment of the present invention may further include calcium pantothenate, vitamin B2, vitamin C, and fructooligosaccharide.

The calcium pantothenate acts as a coenzyme in the living body as a calcium salt fortifying agent, and has an effect of helping normalize tissue function and maintain body growth and health.

The fructooligosaccharide is an oligosaccharide widely present in nature, and is a mixture of components in which 1 to 3 fructose are bonded to glucose. It is used in all foods such as baking and health food. In addition, the pratooligosaccharide added to the feed promotes the growth of fattening pigs and improves the microbial flora in the large intestine, thereby reducing the frequency of diarrhea and removing odorous flavoring metabolites in the large intestine. In addition, it has the effect of improving the digestibility of nutrients in the feed.

The vitamin B2, vitamin C, calcium pantothenate and fructooligosaccharide are all preferably composed of water-soluble raw materials that dissolve well even in cold water. As people, the necessary nutrients for companion animals were hydrated when drinking.

In other words, in order to provide a food composition for companion animals (dog or cat), the present invention undergoes extraction and concentration processes for each raw material using bamboo, beef, and pear as main raw materials, and then, vitamin B2, vitamin C, and pantothenic acid. Calcium and fructooligosaccharide components are contained, and by appropriately diluting them in purified water, it is possible to provide a food composition that is beneficial to the health of companion animals and has high palatability.

Specifically, the food composition for companion animals comprising a bamboo extract according to an embodiment of the present invention is 0.5 to 4% by weight of a bamboo extract, 2 to 4% by weight of a beef extract, 0.1 to 1% by weight of a pear extract relative to 100% by weight of the total composition , vitamin B2 0.00001 to 0.00005% by weight, vitamin C 0.0001 to 0.0003% by weight, calcium pantothenate 0.0005 to 0.001% by weight, fructooligosaccharide 0.01 to 0.06% by weight and the remainder of purified water.

Hereinafter, a method for preparing a food composition for companion animals comprising the extract of Sasa borealis of the present invention will be described.

The method for producing a food composition for companion animals comprising a bamboo extract according to an embodiment of the present invention comprises the steps of (a) preparing a bamboo extract, a beef extract and a pear extract (S100), (b) the bamboo extract, beef It includes the steps of preparing a mixture by mixing the extract and the pear extract (S200) and (c) sterilizing the mixture (S300).

First, (a) is a step (S100) of preparing a bamboo extract, beef extract and pear extract.

Step (a) is a step of hot water extraction, and the extraction may be performed at a temperature of 90 to 100 °C, respectively. For example, the bamboo extract can be extracted by adding 100 to 1,500 parts by weight of water based on 100 parts by weight of the bamboo, and then heating it at a temperature of 90 to 100° C. for 3 to 6 hours, preferably 500 parts by weight of water to 100 parts by weight of the bamboo. After adding 1,000 parts by weight, it can be extracted for 4 hours at a temperature of 90 ℃.

When the extraction temperature is extracted below 90 ℃, there is a problem that the extraction efficiency is lowered and the active ingredient cannot be completely extracted, and when the extraction temperature exceeds 100 ℃, the extraction efficiency is improved but the content of the active ingredient is lowered. The above ranges are preferred.

At this time, the active ingredient may be tricin (Tricin) and p -coumaric acid (p -Coumaric acid).

The pear can also be extracted in the same way.

In the present invention, it may further include the step of preparing an extract using bamboo shoots, beef and pears and concentrating them, respectively, and for example, in the case of the extracted bamboo shoots extract, it can be concentrated to be 10 to 15 Brix, preferably It can be used after concentration to 13 Brix.

The concentration may be performed using a reduced pressure concentrator, but the method is not limited, and the concentration method is a conventional technique, so a description thereof will be omitted.

Next, (b) is a step (S200) of preparing a mixture by mixing the bamboo extract, beef extract and pear extract.

In step (b), 0.5 to 2% by weight of the bamboo extract, 2 to 4% by weight of the beef extract, and 0.1 to 1% by weight of the pear extract may be mixed with respect to 100% by weight of the total composition.

In step (b), one or more of the group consisting of calcium pantothenate, vitamin B2, vitamin C, and fructooligosaccharide may be further included.

Specifically, in the step (S200) of (b), all of the bamboo extract, beef extract, pear extract calcium pantothenate, vitamin B2 and vitamin C may be added and mixed together in purified water, and in another embodiment, the cooktop extract, beef extract and The process of preparing a first mixed solution of the extract by mixing the pear extract, the process of preparing a second mixed solution by adding vitamin B2, vitamin C, calcium pantothenate and fructooligosaccharide to the first mixed solution, and adding purified water to the second mixed solution It can be prepared through the process of mixing.

The (b) step (S200) may be performed using a stirrer, and specifically, may be performed for 10 to 30 minutes using a stirrer at 1,000 to 2,000 rpm.

Finally, (c) is a step (S300) of sterilizing the mixture.

The (c) step (S300) is to remove harmful bacteria present in the mixture, and may be performed at a temperature of 90 to 100° C. for 20 to 40 minutes, preferably at a temperature of 95° C. for 30 minutes. can be performed. Since it is obvious that the sterilization is performed using a sterilizer, a description thereof will be omitted.

The step (c) may further include cooling and packaging the sterilized mixture after step (S300).

In the cooling and packaging step, the sterilized mixture may be put into a PET bottle or a vacuum packaging machine and vacuum-packed after cooling to room temperature by cooling in a natural state in a well-ventilated place.

Hereinafter, it will be described in more detail through Examples and Experimental Examples of the present invention.

<Preparation Examples and Examples>

Preparation Example 1

100 g of bamboo was put into 1,000 ml of distilled water and extracted at a temperature of 20° C. for 2 hours. The extracted bamboo extract was concentrated to 13 Brix to prepare a bamboo concentrate.

Preparation Examples 2 to 6

Except for changing the extraction temperature to 60 ℃ (Preparation Example 2), 70 ℃ (Preparation Example 3), 80 ℃ (Preparation Example 4), 90 ℃ (Preparation Example 5) and 100 ℃ (Preparation Example 6), respectively It was prepared in the same manner as in Preparation Example 1.

Preparation Examples 7 to 9

It was prepared in the same manner as in Preparation Example 5, except that the extraction time was changed to 1 hour (Preparation Example 7), 4 hours (Preparation Example 8), and 6 hours (Preparation Example 9), respectively.

Preparation 10

100 g of pears were added to 1,000 ml of distilled water and extracted at a temperature of 90° C. for 4 hours. The extracted pear extract was concentrated to prepare a pear concentrate.

Example 1

To 955 ml of purified water, 10 ml of rice concentrate, 30 ml of beef concentrate (purchased) and 5 ml of pear concentrate, 0.0003 g of vitamin B2, 0.0016 g of calcium pantothenate, and 0.008 g of vitamin C were added to prepare a mixture. The prepared mixed solution was sterilized at a temperature of 95° C. for 30 minutes to prepare a beverage composition for companion animals including a bamboo extract.

Comparative Example 1

A composition was prepared in the same manner as in Example 1, except that 1 ml of the bamboo concentrate and 964 ml of purified water were used instead of 10 ml of the bamboo concentrate and 955 ml of purified water.

Comparative Example 2

A composition was prepared in the same manner as in Example 1, except that 50 ml of the bamboo concentrate and 915 ml of purified water were used instead of 10 ml of the bamboo concentrate and 955 ml of purified water.

<Experimental example>

Experimental Example 1: Analysis of extraction efficiency and active ingredient content according to temperature

The extraction efficiency and active ingredient content of the bamboo extract according to the temperature were analyzed.

Table 1 below shows the extraction efficiency of the bamboo extract according to the extraction temperature.

Extraction conditions by temperature (℃) division Preparation Example 1 Preparation 2 Preparation 3 Preparation 4 Preparation 5 Preparation 6 Bamboo sample (g) 50.32 50.07 50.35 50.42 50.09 50.81 dry weight (g) 2.62 4.61 5.24 5.80 6.36 6.55 Extraction efficiency (%) 5.2 9.2 10.4 11.5 12.7 12.9 Tricin (mg/g) 0.020 0.095 0.129 0.140 0.263 0.258

Referring to Table 1 above, as for the extraction efficiency, it can be seen that all of Preparation Examples 3 to 6 had an extraction efficiency of 10% or more, and the extraction efficiency of Preparation Example 6 was the most excellent. However, it can be seen that the content of the active ingredient, Tricin, is rather decreased compared to Preparation Example 5 (90°C). Therefore, the extract extracted at 90°C was used for the extract of the bamboo shoots in the present invention.

Experimental Example 2: Analysis of extraction efficiency and active ingredient content according to time

Extraction efficiency and active ingredient content of the bamboo extract according to time were analyzed.

Table 2 below shows the extraction efficiency of the bamboo extract according to the extraction time.

Hourly extraction conditions (h) division Preparation 7 Preparation 5 Preparation 8 Preparation 9 Bamboo sample (g) 50.71 50.09 49.97 50.05 dry weight (g) 5.75 6.36 6.62 6.76 Extraction efficiency (%) 11.34 12.7 13.25 13.50 Tricin (mg/g) 0.214 0.263 0.290 0.319

Referring to Table 2 above, Preparation Example 5, Preparation Example 7 to Preparation Example 9 all showed excellent extraction efficiency, in particular, Preparation Example 8 and Preparation Example 9 contained a high content of active ingredients, and the extraction efficiency was also In other words, it can be seen that in the present invention, the bamboo extract can be extracted at 90° C. for 4 to 6 hours, and the extraction efficiency versus time is preferred in Preparation Example 8, extracted at 90° C. for 4 hours.

Therefore, in the present invention, a bamboo extract extracted at 90° C. for 4 hours was used.

Experimental Example 3: Evaluation of cell viability of bamboo extract

Cell viability of each adipocyte, muscle cell, and hepatocyte for the bamboo extract was evaluated.

Figure 2 is an evaluation of the cell viability of adipocytes, muscle cells, and hepatocytes for the extract of bamboo shoots.

As a result of comparing each cell to which the bamboo was administered and the control cell to which it was not administered, it was confirmed that it did not exhibit cytotoxicity.

In addition, as a result of administering an excessive amount of bamboo grass (2 g per kg body weight per day) to mice, it also did not show cytotoxicity. no change was observed.

Experimental Example 4: AMPK activation assay

Adenosine monophosphate-activated protein kinase (AMPK) activation effects were analyzed in adipocytes (3T3-L1), skeletal muscle cells (L6) and hepatocytes induced by adipogenesis.

3 shows the results of activation of AMPK in adipocytes and skeletal muscle cells of the Z. extracts, and Figure 4 shows the results of activation of AMPK in the hepatocytes induced by the localization of the Z. extract.

3 and 4, as the concentration of the bamboo extract increases, the activity of AMPK, which helps the decomposition of fat in adipocytes (3T3-L1), skeletal muscle cells (L6), and localization-induced liver cells, all increases. that can be checked

Experimental Example 5: Obesity inhibition effect and abdominal fat improvement analysis

In order to confirm the anti-obesity effect of bamboo extract, weight change, abdominal fat improvement effect, and fatty liver improvement effect were analyzed.

1. Weight change and abdominal fat improvement effect analysis

Figure 5 shows the change in the body weight of the mice fed the bamboo extract, Figure 6 shows the effect of improving the abdominal fat of the mice fed the bamboo extract.

Changes in body weight and abdominal fat were observed in mice fed a normal diet (ND), mice fed a high-fat diet (HFD), and mice fed a high-fat diet and cod extract (HFD+SQE) for 70 days.

Referring to FIGS. 5 and 6 , it can be seen that the weight change of the mice fed the high-fat diet and the bamboo extract together (HFD+SQE) was smaller than that of the mice fed the high-fat diet (HFD). That is, it can be confirmed that the bamboo extract has an effect of suppressing obesity.

In addition, as a result of analyzing the effect of improving abdominal fat in mice fed a general diet (ND) and mice fed a high-fat diet and cod extract (HFD+SQE), similar improvement effects were shown.

2. Analysis of the effect of improving fatty liver

7 shows an image, GPT, and GOT analyzing the effect of improving fatty liver in mice fed the extract of Zordi.

Liver images of mice fed a general diet (ND), mice fed a high-fat diet (HFD), and mice fed a high-fat diet and cod extract (HFD+SQE) for 70 days were photographed, and GPT and GOT levels were measured. .

Referring to FIG. 7 , it was observed that the liver values (GPT and GOT) of mice co-administered with the bamboo extract were improved at a level similar to that of normal mice, and the weight of the liver was also similar to that of normal mice. In other words, it can be confirmed that the bamboo extract is effective in improving liver function.

Experimental Example 6: Dog Preference Analysis

The beverage compositions prepared according to Example 1 and Comparative Examples 1 to 3 were supplied to five test group individuals (dogs), and the drinking state was observed and shown in Table 3 (Drink well: ○, drink a little; △, do not drink. : ×).

division Example 1 Comparative Example 1 Comparative Example 2 Jindo Mix ○ △ × golden retriever ○ × × mixed dog ○ △ × poodle ○ × × bichon ○ × ×

As can be seen in Table 3 above, it can be seen that Comparative Examples 1 and 2 have a low preference, whereas in the case of Example 1, it can be confirmed that a high preference is displayed regardless of the species. By using a food composition for companion animals containing

S100: extract preparation step
S200: mixture preparation step
S300: Sterilization step

Claims (11)

A food composition for companion animals comprising an extract of Sasa borealis . According to claim 1,
A food composition for companion animals, characterized in that it contains 0.5 to 4% by weight of the bamboo extract relative to 100% by weight of the total composition. According to claim 1,
The composition is a food composition for companion animals, characterized in that it has an anti-obesity effect. According to claim 1,
The composition is a food composition for companion animals, characterized in that it further comprises a beef extract and a pear extract. 5. The method of claim 4,
Food composition for companion animals, characterized in that it comprises 2 to 4% by weight of the beef extract and 0.1 to 1% by weight of the pear extract relative to 100% by weight of the total composition. According to claim 1,
The composition is a food composition for companion animals, characterized in that it further comprises calcium pantothenate, vitamin B2, vitamin C and fructooligosaccharide. According to claim 1,
The food is a food composition for companion animals, characterized in that the beverage. (a) preparing a bamboo extract, beef extract and pear extract;
(b) preparing a mixture by mixing the bamboo extract, beef extract and pear extract; and
(c) a method for producing a food composition for companion animals comprising the step of sterilizing the mixture. 9. The method of claim 8,
Each of the extracts in step (a) is a method for producing a food composition for companion animals, characterized in that the hot water extraction at a temperature of 90 ~ 100 ℃. 9. The method of claim 8,
Step (b) is,
100% by weight of the total composition, 0.5 to 2% by weight of the bamboo extract, 2 to 4% by weight of the beef extract, and 0.1 to 1% by weight of the pear extract, a method for producing a food composition for companion animals. 9. The method of claim 8,
Step (c) is,
A method for producing a food composition for companion animals, characterized in that it is carried out for 20 to 40 minutes at a temperature of 90 to 100 ° C.

Images