KR20220108055A - 단일-세포 단백질 및 핵산 서열분석 방법 - Google Patents

단일-세포 단백질 및 핵산 서열분석 방법 Download PDF

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Publication number
KR20220108055A
KR20220108055A KR1020227017591A KR20227017591A KR20220108055A KR 20220108055 A KR20220108055 A KR 20220108055A KR 1020227017591 A KR1020227017591 A KR 1020227017591A KR 20227017591 A KR20227017591 A KR 20227017591A KR 20220108055 A KR20220108055 A KR 20220108055A
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KR
South Korea
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barcoded
sample
polypeptide
molecule
amino acid
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KR1020227017591A
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English (en)
Korean (ko)
Inventor
매튜 다이어
브라이언 리드
Original Assignee
퀀텀-에스아이 인코포레이티드
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Publication of KR20220108055A publication Critical patent/KR20220108055A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
KR1020227017591A 2019-10-28 2020-10-28 단일-세포 단백질 및 핵산 서열분석 방법 KR20220108055A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962926991P 2019-10-28 2019-10-28
US62/926,991 2019-10-28
US202062991425P 2020-03-18 2020-03-18
US62/991,425 2020-03-18
PCT/US2020/057656 WO2021086913A1 (en) 2019-10-28 2020-10-28 Methods of single-cell protein and nucleic acid sequencing

Publications (1)

Publication Number Publication Date
KR20220108055A true KR20220108055A (ko) 2022-08-02

Family

ID=73598182

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227017591A KR20220108055A (ko) 2019-10-28 2020-10-28 단일-세포 단백질 및 핵산 서열분석 방법

Country Status (10)

Country Link
US (1) US20210139973A1 (zh)
EP (1) EP4041910A1 (zh)
JP (1) JP2023500485A (zh)
KR (1) KR20220108055A (zh)
CN (1) CN114981448A (zh)
AU (1) AU2020374885A1 (zh)
BR (1) BR112022008075A2 (zh)
CA (1) CA3159560A1 (zh)
MX (1) MX2022005093A (zh)
WO (1) WO2021086913A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2021005757A (es) 2018-11-15 2021-08-11 Quantum Si Inc Metodos y composiciones para la secuenciacion de proteinas.
CN114981448A (zh) * 2019-10-28 2022-08-30 宽腾矽公司 单细胞蛋白质和核酸测序的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496294A1 (en) 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
WO2010065322A1 (en) * 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
NL2009191C2 (en) * 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
US9435810B2 (en) * 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
EP3194980A4 (en) * 2014-09-15 2018-07-04 Board of Regents, The University of Texas System Improved single molecule peptide sequencing
JP7120630B2 (ja) * 2016-05-02 2022-08-17 エンコディア， インコーポレイテッド 核酸エンコーディングを使用した巨大分子解析
WO2018045186A1 (en) * 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
US11072816B2 (en) * 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
WO2019089846A1 (en) * 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
US11841371B2 (en) * 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
AU2019355579A1 (en) * 2018-10-05 2021-05-06 Board Of Regents, The University Of Texas System Solid-phase N-terminal peptide capture and release
US20200300861A1 (en) * 2019-03-22 2020-09-24 Augmenta Bioworks, Inc. Isolation of Single Cells and Uses Thereof
WO2020243643A1 (en) * 2019-05-31 2020-12-03 President And Fellows Of Harvard College Systems and methods for ms1-based mass identification including super-resolution techniques
CN114555810A (zh) * 2019-09-13 2022-05-27 谷歌有限责任公司 用于蛋白质和肽测序的方法和组合物
CN114981448A (zh) * 2019-10-28 2022-08-30 宽腾矽公司 单细胞蛋白质和核酸测序的方法

Also Published As

Publication number Publication date
CA3159560A1 (en) 2021-05-06
AU2020374885A1 (en) 2022-06-02
JP2023500485A (ja) 2023-01-06
EP4041910A1 (en) 2022-08-17
CN114981448A (zh) 2022-08-30
MX2022005093A (es) 2022-08-11
BR112022008075A2 (pt) 2022-07-12
WO2021086913A1 (en) 2021-05-06
US20210139973A1 (en) 2021-05-13

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