KR20220106738A - 조작된 리파제 변이체 - Google Patents
조작된 리파제 변이체 Download PDFInfo
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Abstract
본 발명은 조작된 리파제 폴리펩티드 및 이의 조성물을 제공한다. 조작된 리파제 폴리펩티드는 개선된 열안정성, 프로테아제 안정성, 및 산성(pH <7) 및 염기성(pH >7) 조건을 포함한 다양한 pH 조건 하에서의 안정성을 제공하도록 최적화되었다. 본 발명은 또한 치료 및/또는 영양 목적을 위한 조작된 리파제 폴리펩티드를 포함하는 조성물의 용도에 관한 것이다. 본 발명은 또한 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 뿐만 아니라 조작된 폴리뉴클레오티드 및 리파제 폴리펩티드의 제조 방법을 제공한다.
Description
본 출원은 2019년 8월 30일에 출원된 미국 가출원 제62/894,019호를 우선권으로 주장하며, 상기 가출원은 모든 목적상 그 전체가 참조로 본원에 포함된다.
발명의 분야
본 발명은 조작된 리파제 폴리펩티드 및 이의 조성물을 제공한다. 조작된 리파제 폴리펩티드는 개선된 열안정성, 프로테아제 안정성, 및 산성(pH <7) 조건을 포함한 다양한 pH 조건 하에서의 안정성을 제공하도록 최적화되었다. 본 발명은 또한 치료 및/또는 영양 목적을 위한 조작된 리파제 폴리펩티드를 포함하는 조성물의 용도에 관한 것이다. 본 발명은 또한 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 뿐만 아니라 조작된 폴리뉴클레오티드 및 리파제 폴리펩티드의 제조 방법을 제공한다.
서열 목록, 표 또는 컴퓨터 프로그램에 대한 참조
서열 목록의 공식 사본은 EFS-Web을 통해 ASCII 포맷의 텍스트 파일로 본 명세서와 동시에 제출되며, 파일명은 "CX7-187WO2_ST25.txt"이고, 작성 날짜는 2020년 8월 27일이고, 크기는 4.30 메가바이트이다. EFS-Web을 통해 제출된 서열 목록은 본 명세서의 일부이고, 전체가 본원에 참조로 포함된다.
췌장 효소 대체 요법(PERT)은 췌장 효소 기능부전(PEI)의 치료에 사용된다. 췌장염, 낭포성 섬유증, 복강 질환, 염증성 장 질환 및 췌장암을 포함한 다양한 장애는 십이지장으로의 췌장 효소 분비 감소의 결과로 PEI를 유발할 수 있다. 이는 음식의 소화 불량, 장에서 지방, 단백질, 탄수화물 및 비타민의 부적절한 흡수를 초래하여 영양실조로 이어질 수 있다. 경구 투여된 PERT 치료가 현재 이용 가능하지만, 위장관에서 PERT의 불충분한 활성 및/또는 현행 치료 프로토콜과 관련된 상당한 환약 부담으로 인한 환자의 불충분한 요법 순응도 때문에 일부 사람들에서 병태가 완화되지 않을 수 있다. 일부 경우에는, 지방 흡수 계수(CFA) 및/또는 질소 흡수 계수(CNA)가 건강한 환자보다 열등하여 체중 감소 및 다른 건강 문제가 발생한다. 따라서, 개선된 PERT 치료에 대한 요구가 당업계에 남아 있다.
본 발명은 조작된 리파제 폴리펩티드 및 이의 조성물을 제공한다. 조작된 리파제 폴리펩티드는 개선된 열안정성, 프로테아제 안정성, 및 산성(pH <7) 조건을 포함한 다양한 pH 조건 하에서의 안정성을 제공하도록 최적화되었다. 본 발명은 또한 치료 및/또는 영양 목적을 위한 조작된 리파제 폴리펩티드를 포함하는 조성물의 용도에 관한 것이다. 본 발명은 또한 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 뿐만 아니라 조작된 폴리뉴클레오티드 및 리파제 폴리펩티드의 제조 방법을 제공한다.
본 발명은 서열번호 2에 대해 적어도 70%, 적어도 75%, 적어도 80%, 적어도 85%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 또는 적어도 99% 서열 동일성을 포함하는 아미노산 서열을 포함하는 재조합 리파제 및/또는 생물학적 활성 재조합 리파제 단편을 제공한다. 일부 실시양태에서, 재조합 리파제 및/또는 생물학적 활성 재조합 리파제 단편은 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함한다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 2, 3, 4, 22, 27/46/97/136/149/385, 27/46/385, 27/70/136/231/385, 27/136/323/385, 27/149, 27/149/385, 27/385, 34, 38, 41, 44, 46/70, 46/149/183/231/385, 48, 70, 73, 73/305, 82, 82/94/101/194/199, 82/94/199, 83, 85, 87, 89, 92, 94, 96, 97/149/385, 135, 136/385, 140, 141, 142, 144, 146, 149, 149/231/385, 151, 174, 175, 178, 181, 183/385, 189, 194, 194/199, 195, 195/231/385, 199, 199/213, 210, 212, 213, 213/330, 216, 218, 219, 226, 231/385, 238, 247, 250, 270, 274, 281, 292, 296, 300, 308, 330, 338, 379, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 2H, 2M, 2T, 3A, 3G, 3R, 3S, 4M, 4W, 4Y, 22N, 27V/46T/97A/136V/149E/385P, 27V/46T/385P, 27V/70N/136V/231E/385P, 27V/136V/323I/385P, 27V/149E, 27V/149E/385P, 27V/385P, 34D, 38A, 38H, 38L, 41V, 44E, 46T/70N, 46T/149E/183L/231E/385P, 48V, 70N, 73C/305I, 73I, 73R, 82C, 82E, 82E/94S/101S/194N/199L, 82E/94S/199L, 82F, 82G, 82L, 83G, 83K, 85I, 85W, 87R, 89A, 89T, 89V, 89W, 92A, 94S, 96E, 96N, 96S, 97A/149E/385P, 135G, 135Q, 135S, 135T, 135V, 136V/385P, 140T, 141A, 141F, 141L, 141S, 142A, 142F, 142I, 142L, 144M, 144R, 144W, 146A, 146F, 146S, 146Y, 149E, 149E/231E/385P, 149K, 149R, 151A, 151I, 151L, 174H, 174R, 175G, 175L, 175R, 178R, 178T, 181H, 183L/385P, 189A, 189E, 189W, 194N, 194N/199L, 194T, 195D/231E/385P, 195V, 199L, 199L/213A, 210A, 210V, 212C, 212G, 212R, 212T, 213A/330T, 213H, 216R, 216T, 216W, 218D, 218G, 218I, 218M, 218T, 219C, 219G, 219K, 219R, 226R, 231E/385P, 238S, 247R, 250T, 270V, 274D, 281K, 281R, 292A, 292C, 292L, 292V, 296M, 296R, 300A, 300D, 300T, 308A, 330Y, 338N, 379T, 385A, 385C, 385D, 385P, 385R, 및 385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 E2H, E2M, E2T, T3A, T3G, T3R, T3S, S4M, S4W, S4Y, G22N, Y27V/R46T/Y97A/Y136V/T149E/F385P, Y27V/R46T/F385P, Y27V/K70N/Y136V/Q231E/F385P, Y27V/Y136V/K323I/F385P, Y27V/T149E, Y27V/T149E/F385P, Y27V/F385P, K34D, F38A, F38H, F38L, N41V, G44E, R46T/K70N, R46T/T149E/F183L/Q231E/F385P, Y48V, K70N, T73C/V305I, T73I, T73R, K82C, K82E, K82E/P94S/D101S/K194N/I199L, K82E/P94S/I199L, K82F, K82G, K82L, E83G, E83K, G85I, G85W, A87R, F89A, F89T, F89V, F89W, T92A, P94S, I96E, I96N, I96S, Y97A/T149E/F385P, Y135G, Y135Q, Y135S, Y135T, Y135V, Y136V/F385P, P140T, E141A, E141F, E141L, E141S, E142A, E142F, E142I, E142L, I144M, I144R, I144W, P146A, P146F, P146S, P146Y, T149E, T149E/Q231E/F385P, T149K, T149R, G151A, G151I, G151L, E174H, E174R, Q175G, Q175L, Q175R, S178R, S178T, K181H, F183L/F385P, S189A, S189E, S189W, K194N, K194N/I199L, K194T, Q195D/Q231E/F385P, Q195V, I199L, I199L/P213A, K210A, K210V, Q212C, Q212G, Q212R, Q212T, P213A/N330T, P213H, S216R, S216T, S216W, H218D, H218G, H218I, H218M, H218T, A219C, A219G, A219K, A219R, T226R, Q231E/F385P, Y238S, E247R, Q250T, R270V, T274D, G281K, G281R, M292A, M292C, M292L, M292V, S296M, S296R, Q300A, Q300D, Q300T, R308A, N330Y, K338N, N379T, F385A, F385C, F385D, F385P, F385R, 및 F385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 3/4/96/296/300, 3/292/296, 4, 4/11/149/292/296/300, 4/96/149/231/296/300, 4/96/149/292/296/300, 4/96/219/296, 4/96/231/296/300, 4/96/292/296/300, 4/149/174, 4/174/219/292/296, 4/231/296/300, 27, 27/34/82, 27/73/82/218, 27/89, 27/89/178, 27/89/218, 27/218, 34/73/218, 34/82, 34/218, 73/82, 73/82/183, 94/146/175, 96/149/174/292/296, 96/149/231/292/296, 96/149/292/296, 96/174/219/231/292/296, 96/231/296, 146/175/189/281, 149/174/292/296, 149/174/300, 149/219/231/292/296/300, 149/231/292/296, 149/231/292/296/300, 149/296, 149/296/300, 174/231, 174/296, 218, 231, 231/292/296, 231/292/296/300, 231/296, 231/300, 292/296, 292/296/300, 296, 및 296/300으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 3S/4W/96E/296R/300D, 3S/292L/296R, 4W, 4W/11A/149E/292L/296R/300D, 4W/96E/149E/231E/296R/300D, 4W/96E/149E/292L/296R/300D, 4W/96E/219K/296R, 4W/96E/231E/296R/300D, 4W/96E/292L/296R/300D, 4W/149E/174R, 4W/174H/219K/292L/296R, 4W/231E/296R/300D, 27V, 27V/34D/82E, 27V/73I/82L/218I, 27V/73I/82L/218M, 27V/89T, 27V/89T/178P, 27V/89T/218I, 27V/218I, 34D/73I/218I, 34D/82C, 34D/218M, 73I/82C, 73I/82L/183L, 94S/146F/175G, 96E/149E/174H/292L/296R, 96E/149E/231E/292L/296R, 96E/149E/292L/296R, 96E/174H/219K/231E/292L/296R, 96E/231E/296R, 146F/175G/189A/281K, 149E/174H/292L/296R, 149E/174H/300D, 149E/219K/231E/292L/296R/300D, 149E/231E/292L/296R, 149E/231E/292L/296R/300D, 149E/296R, 149E/296R/300D, 174H/296R, 174R/231E, 218I, 231E, 231E/292L/296R, 231E/292L/296R/300D, 231E/296R, 231E/300D, 292L/296R, 292L/296R/300D, 296R, 및 296R/300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 T3S/S4W/I96E/S296R/Q300D, T3S/M292L/S296R, S4W, S4W/V11A/T149E/M292L/S296R/Q300D, S4W/I96E/T149E/Q231E/S296R/Q300D, S4W/I96E/T149E/M292L/S296R/Q300D, S4W/I96E/A219K/S296R, S4W/I96E/Q231E/S296R/Q300D, S4W/I96E/M292L/S296R/Q300D, S4W/T149E/E174R, S4W/E174H/A219K/M292L/S296R, S4W/Q231E/S296R/Q300D, Y27V, Y27V/K34D/K82E, Y27V/T73I/K82L/H218I, Y27V/T73I/K82L/H218M, Y27V/F89T, Y27V/F89T/S178P, Y27V/F89T/H218I, Y27V/H218I, K34D/T73I/H218I, K34D/K82C, K34D/H218M, T73I/K82C, T73I/K82L/F183L, P94S/P146F/Q175G, I96E/T149E/E174H/M292L/S296R, I96E/T149E/Q231E/M292L/S296R, I96E/T149E/M292L/S296R, I96E/E174H/A219K/Q231E/M292L/S296R, I96E/Q231E/S296R, P146F/Q175G/S189A/G281K, T149E/E174H/M292L/S296R, T149E/E174H/Q300D, T149E/A219K/Q231E/M292L/S296R/Q300D, T149E/Q231E/M292L/S296R, T149E/Q231E/M292L/S296R/Q300D, T149E/S296R, T149E/S296R/Q300D, E174H/S296R, E174R/Q231E, H218I, Q231E, Q231E/M292L/S296R, Q231E/M292L/S296R/Q300D, Q231E/S296R, Q231E/Q300D, M292L/S296R, M292L/S296R/Q300D, S296R, 및 S296R/Q300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 4, 4/27/189/300, 4/70, 4/102, 4/137, 4/175/189/218, 4/175/189/300, 4/175/218/224, 4/175/218/373, 4/185, 4/189/218/373, 4/193, 4/218/300/369/373, 4/228, 4/233, 4/243, 4/271, 4/303, 4/334, 4/336, 4/375, 25, 27/82/174/175/189/218/300/369/373, 27/82/174/175/218/300/369, 27/82/174/175/218/300/373/382, 27/82/174/218/300/373, 27/189/218/373, 28, 33, 99, 102, 104, 134, 153, 174/175/189, 174/373, 175/189/218/300/373, 175/189/373, 175/218, 175/218/382, 189/218/300/373, 191, 193, 231, 233, 243, 293, 303, 331, 336, 339, 368, 및 373으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 4W, 4W/27V/189A/300D, 4W/70H, 4W/102T, 4W/137R, 4W/175G/189A/218M, 4W/175G/189A/300D, 4W/175G/218M/224A, 4W/175G/218M/373F, 4W/185L, 4W/189A/218M/373F, 4W/193T, 4W/218M/300D/369N/373F, 4W/228E, 4W/233R, 4W/243R, 4W/271D, 4W/303P, 4W/334T, 4W/336S, 4W/336T, 4W/375A, 25V, 27V/82C/174H/175G/189A/218M/300D/369N/373F, 27V/82C/174H/175G/218M/300D/369N, 27V/82C/174H/175G/218M/300D/373F/382M, 27V/82C/174H/218M/300D/373F, 27V/189A/218M/373F, 28N, 33Y, 99D, 102L, 104H, 134R, 153G, 174H/175L/189A, 174H/373F, 175G/189A/218M/300D/373F, 175G/218M, 175G/218M/382M, 175L/189A/373F, 189A/218M/300D/373F, 191L, 193L, 231Q, 233G, 233S, 233W, 243R, 293R, 303P, 331R, 336R, 339K, 368A, 368T, 및 373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 S4W, S4W/Y27V/S189A/Q300D, S4W/K70H, S4W/E102T, S4W/S137R, S4W/Q175G/S189A/H218M, S4W/Q175G/S189A/Q300D, S4W/Q175G/H218M/V224A, S4W/Q175G/H218M/Y373F, S4W/I185L, S4W/S189A/H218M/Y373F, S4W/Q193T, S4W/H218M/Q300D/S369N/Y373F, S4W/P228E, S4W/N233R, S4W/L243R, S4W/G271D, S4W/A303P, S4W/D334T, S4W/A336S, S4W/A336T, S4W/N375A, L25V, Y27V/K82C/E174H/Q175G/S189A/H218M/Q300D/S369N/Y373F, Y27V/K82C/E174H/Q175G/H218M/Q300D/S369N, Y27V/K82C/E174H/Q175G/H218M/Q300D/Y373F/H382M, Y27V/K82C/E174H/H218M/Q300D/Y373F, Y27V/S189A/H218M/Y373F, R28N, L33Y, Q99D, E102L, N104H, E134R, N153G, E174H/Q175L/S189A, E174H/Y373F, Q175G/S189A/H218M/Q300D/Y373F, Q175G/H218M, Q175G/H218M/H382M, Q175L/S189A/Y373F, S189A/H218M/Q300D/Y373F, A191L, Q193L, E231Q, N233G, N233S, N233W, L243R, Q293R, A303P, S331R, A336R, Q339K, F368A, F368T, 및 Y373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 26, 33, 33/174/193/243, 33/174/334, 33/175/218/303, 33/175/218/334/339, 33/193, 34, 38, 46, 48, 70/271/293/334, 144, 174, 174/175/193, 174/175/218/339, 174/193/303/375, 174/193/375, 174/218/233/271/293/303, 174/218/271/303, 175/193/218/233/243/375, 175/193/218/243, 175/218/375, 181, 189, 193, 193/218/243, 193/271/303/334, 193/293/303, 194, 195, 199, 210, 218, 218/243, 218/243/303/334, 218/303, 225, 238, 274, 281, 및 330으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 26A, 26R, 26S, 33Y, 33Y/174H/193L/243R, 33Y/174H/334T, 33Y/175G/218M/303P, 33Y/175G/218M/334T/339K, 33Y/193L, 34L, 38A, 38G, 46P, 48L, 70H/271D/293R/334T, 144L, 174H, 174H/175G/193L, 174H/175G/218M/339K, 174H/193L/303P/375A, 174H/193L/375A, 174H/218M/233R/271D/293R/303P, 174H/218M/271D/303P, 174R, 175G/193L/218M/233R/243R/375A, 175G/193L/218M/243R, 175G/218M/375A, 181Q, 189H, 193L, 193L/218M/243R, 193L/271D/303P/334T, 193L/293R/303P, 194T, 195I, 195L, 195Y, 199H, 210V, 218C, 218D, 218M, 218M/243R, 218M/243R/303P/334T, 218M/303P, 218P, 225L, 238W, 274D, 281K, 281P, 330F, 및 330H로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 G26A, G26R, G26S, L33Y, L33Y/E174H/Q193L/L243R, L33Y/E174H/D334T, L33Y/L175G/H218M/A303P, L33Y/L175G/H218M/D334T/Q339K, L33Y/Q193L, K34L, F38A, F38G, R46P, Y48L, K70H/G271D/Q293R/D334T, I144L, E174H, E174H/L175G/Q193L, E174H/L175G/H218M/Q339K, E174H/Q193L/A303P/N375A, E174H/Q193L/N375A, E174H/H218M/N233R/G271D/Q293R/A303P, E174H/H218M/G271D/A303P, E174R, L175G/Q193L/H218M/N233R/L243R/N375A, L175G/Q193L/H218M/L243R, L175G/H218M/N375A, K181Q, A189H, Q193L, Q193L/H218M/L243R, Q193L/G271D/A303P/D334T, Q193L/Q293R/A303P, K194T, Q195I, Q195L, Q195Y, I199H, K210V, H218C, H218D, H218M, H218M/L243R, H218M/L243R/A303P/D334T, H218M/A303P, H218P, M225L, Y238W, T274D, G281K, G281P, N330F, 및 N330H로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 34/38/174/193/195/243/281/303/330, 34/38/174/225/303, 34/38/174/303/345, 34/174, 34/174/193/195/243/281, 34/174/193/195/303/330, 34/193/195/225/243, 34/193/243/303/330, 34/281/330, 38, 38/174/193/195/243/330, 38/174/193/225/274/283/303/330, 38/174/193/303, 38/174/195/281/330, 38/174/225/243/281/330, 38/174/281, 38/174/281/303, 38/174/281/303/345, 38/193/195/225/303/345, 38/195/243/303, 38/195/281/303/330, 38/195/281/330, 38/195/303, 49, 49/51/98/252/311, 49/51/123/252/344, 49/98/120/252/344, 49/120/252/344, 49/123/252/344, 49/123/264/344, 49/123/311, 49/252/344, 49/311/344, 49/344, 51, 51/252/344, 51/344, 98, 98/344, 123/252/344, 129, 160, 161, 174/193/195/225, 174/193/303/330, 174/195/225/281/303/330/345, 174/195/243/281/345, 174/195/281/303, 174/225/243/281/303/345, 174/281/330, 174/303, 195/225/303/330, 252, 268, 및 344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 34L/38A/174G/193L/195L/243R/281P/303P/330F, 34L/38A/174R/303P/345I, 34L/38G/174R/225L/303P, 34L/174G, 34L/174G/193L/195L/243R/281P, 34L/174H/193L/195I/303P/330F, 34L/193L/195I/225L/243R, 34L/193L/243R/303P/330F, 34L/281P/330H, 38A/174H/193L/225L/274D/283I/303P/330F, 38A/174R/281K/303P/345I, 38A/193L/195L/225L/303P/345I, 38A/195I/303P, 38A/195Y/281K/303P/330F, 38G, 38G/174G/193L/303P, 38G/174G/281K/303P, 38G/174H/195L/281P/330F, 38G/174H/225L/243R/281K/330H, 38G/174H/281K, 38G/174R/193L/195L/243R/330H, 38G/195I/281P/330F, 38G/195Y/243R/303P, 49S, 49T, 49T/51A/98P/252V/311W, 49T/51A/123Q/252V/344H, 49T/98P/120T/252V/344H, 49T/120T/252V/344H, 49T/123Q/252V/344H, 49T/123Q/264S/344H, 49T/123Q/311W, 49T/252V/344H, 49T/311W/344H, 49T/344H, 51A/252V/344H, 51A/344H, 51V, 98P/344H, 98R, 123Q/252V/344H, 129F, 160T, 161I, 174G/195I/225L/281K/303P/330F/345I, 174G/225L/243R/281P/303P/345I, 174H/195I/243R/281K/345I, 174H/281P/330H, 174R/193L/195I/225L, 174R/193L/303P/330H, 174R/195I/281K/303P, 174R/303P, 195Y/225L/303P/330H, 252V, 268T, 344I, 344V, 및 344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 K34L/F38A/E174G/Q193L/Q195L/L243R/G281P/A303P/N330F, K34L/F38A/E174R/A303P/F345I, K34L/F38G/E174R/M225L/A303P, K34L/E174G, K34L/E174G/Q193L/Q195L/L243R/G281P, K34L/E174H/Q193L/Q195I/A303P/N330F, K34L/Q193L/Q195I/M225L/L243R, K34L/Q193L/L243R/A303P/N330F, K34L/G281P/N330H, F38A/E174H/Q193L/M225L/T274D/M283I/A303P/N330F, F38A/E174R/G281K/A303P/F345I, F38A/Q193L/Q195L/M225L/A303P/F345I, F38A/Q195I/A303P, F38A/Q195Y/G281K/A303P/N330F, F38G, F38G/E174G/Q193L/A303P, F38G/E174G/G281K/A303P, F38G/E174H/Q195L/G281P/N330F, F38G/E174H/M225L/L243R/G281K/N330H, F38G/E174H/G281K, F38G/E174R/Q193L/Q195L/L243R/N330H, F38G/Q195I/G281P/N330F, F38G/Q195Y/L243R/A303P, V49S, V49T, V49T/T51A/G98P/M252V/L311W, V49T/T51A/E123Q/M252V/S344H, V49T/G98P/M120T/M252V/S344H, V49T/M120T/M252V/S344H, V49T/E123Q/M252V/S344H, V49T/E123Q/T264S/S344H, V49T/E123Q/L311W, V49T/M252V/S344H, V49T/L311W/S344H, V49T/S344H, T51A/M252V/S344H, T51A/S344H, T51V, G98P/S344H, G98R, E123Q/M252V/S344H, S129F, S160T, L161I, E174G/Q195I/M225L/G281K/A303P/N330F/F345I, E174G/M225L/L243R/G281P/A303P/F345I, E174H/Q195I/L243R/G281K/F345I, E174H/G281P/N330H, E174R/Q193L/Q195I/M225L, E174R/Q193L/A303P/N330H, E174R/Q195I/G281K/A303P, E174R/A303P, Q195Y/M225L/A303P/N330H, M252V, S268T, S344I, S344V, 및 S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 11/16/67/168/180/287, 11/16/237/241/287, 11/67/168/287, 16/67/154/168/237/241/287, 16/67/237/287/291, 16/168/177, 24, 24/278, 34/49/161/193/243/344, 34/49/161/193/344, 34/49/161/243/344, 34/49/161/252/268/344, 34/49/193/243/252/344, 34/49/193/344, 34/49/243/252, 34/49/252/268/344, 34/161/193/243/252/268, 34/161/193/243/268/344, 34/161/193/243/344, 34/161/193/252/268/344, 34/161/193/252/344, 34/161/193/268/344, 34/161/243/281/344, 34/161/344, 34/193/243/252/344, 34/193/252/268/344, 34/193/268/281, 34/252/268/281/344, 34/252/344, 49/161/193/243/252/344, 49/161/193/252/281/344, 49/161/243/252/344, 49/193/197/243/252/281/344, 49/193/243/252/344, 49/193/243/344, 49/243/344, 67/154/237/287, 67/168/237, 67/168/237/287, 67/177/237, 154/237/286/287, 154/286/287, 161/193/252/344, 161/193/344, 161/344, 168/237, 186/187/278, 193/243/281/344, 193/252/268/344, 193/252/281/344, 237, 237/287/291, 및 281/344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 11I/16F/67A/168T/180V/287L, 11I/16F/237Q/241S/287L, 11I/67A/168T/287L, 16F/67A/154Y/168T/237Q/241S/287L, 16F/67A/237Q/287L/291A, 16F/168T/177L, 24M, 24M/278L, 34K/49T/161I/193L/243R/344H, 34K/49T/161I/193L/243R/344V, 34K/49T/161I/193L/344W, 34K/49T/161I/243R/344H, 34K/49T/161I/252V/268T/344V, 34K/49T/193L/243R/252V/344W, 34K/49T/193L/344H, 34K/49T/193L/344W, 34K/49T/243R/252V, 34K/49T/252V/268T/344V, 34K/161I/193L/243R/252V/268T, 34K/161I/193L/243R/268T/344V, 34K/161I/193L/243R/344W, 34K/161I/193L/252V/268T/344H, 34K/161I/193L/252V/268T/344V, 34K/161I/193L/252V/344H, 34K/161I/193L/252V/344V, 34K/161I/193L/252V/344W, 34K/161I/193L/268T/344H, 34K/161I/243R/281P/344V, 34K/161I/344V, 34K/161I/344W, 34K/193L/243R/252V/344W, 34K/193L/252V/268T/344H, 34K/193L/268T/281P, 34K/252V/268T/281P/344V, 34K/252V/344V, 49T/161I/193L/243R/252V/344W, 49T/161I/193L/252V/281P/344H, 49T/161I/243R/252V/344V, 49T/193L/197G/243R/252V/281P/344H, 49T/193L/243R/252V/344V, 49T/193L/243R/344W, 49T/243R/344V, 67A/154Y/237Q/287L, 67A/168T/237Q, 67A/168T/237Q/287L, 67A/177L/237Q, 154Y/237Q/286V/287L, 154Y/286V/287L, 161I/193L/252V/344V, 161I/193L/344W, 161I/344W, 168T/237Q, 186T/187A/278L, 193L/243R/281P/344W, 193L/252V/268T/344V, 193L/252V/281P/344H, 237Q, 237Q/287L/291A, 및 281P/344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 V11I/L16F/Y67A/S168T/I180V/F287L, V11I/L16F/A237Q/T241S/F287L, V11I/Y67A/S168T/F287L, L16F/Y67A/W154Y/S168T/A237Q/T241S/F287L, L16F/Y67A/A237Q/F287L/S291A, L16F/S168T/V177L, F24M, F24M/Y278L, L34K/V49T/L161I/Q193L/L243R/S344H, L34K/V49T/L161I/Q193L/L243R/S344V, L34K/V49T/L161I/Q193L/S344W, L34K/V49T/L161I/L243R/S344H, L34K/V49T/L161I/M252V/S268T/S344V, L34K/V49T/Q193L/L243R/M252V/S344W, L34K/V49T/Q193L/S344H, L34K/V49T/Q193L/S344W, L34K/V49T/L243R/M252V, L34K/V49T/M252V/S268T/S344V, L34K/L161I/Q193L/L243R/M252V/S268T, L34K/L161I/Q193L/L243R/S268T/S344V, L34K/L161I/Q193L/L243R/S344W, L34K/L161I/Q193L/M252V/S268T/S344H, L34K/L161I/Q193L/M252V/S268T/S344V, L34K/L161I/Q193L/M252V/S344H, L34K/L161I/Q193L/M252V/S344V, L34K/L161I/Q193L/M252V/S344W, L34K/L161I/Q193L/S268T/S344H, L34K/L161I/L243R/G281P/S344V, L34K/L161I/S344V, L34K/L161I/S344W, L34K/Q193L/L243R/M252V/S344W, L34K/Q193L/M252V/S268T/S344H, L34K/Q193L/S268T/G281P, L34K/M252V/S268T/G281P/S344V, L34K/M252V/S344V, V49T/L161I/Q193L/L243R/M252V/S344W, V49T/L161I/Q193L/M252V/G281P/S344H, V49T/L161I/L243R/M252V/S344V, V49T/Q193L/S197G/L243R/M252V/G281P/S344H, V49T/Q193L/L243R/M252V/S344V, V49T/Q193L/L243R/S344W, V49T/L243R/S344V, Y67A/W154Y/A237Q/F287L, Y67A/S168T/A237Q, Y67A/S168T/A237Q/F287L, Y67A/V177L/A237Q, W154Y/A237Q/L286V/F287L, W154Y/L286V/F287L, L161I/Q193L/M252V/S344V, L161I/Q193L/S344W, L161I/S344W, S168T/A237Q, H186T/L187A/Y278L, Q193L/L243R/G281P/S344W, Q193L/M252V/S268T/S344V, Q193L/M252V/G281P/S344H, A237Q, A237Q/F287L/S291A, 및 G281P/S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24/168/252/281, 24/237/287/344, 24/252, 24/252/287, 24/344, 213/252/278/344, 252/278/344, 252/287/344, 및 281로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24M/168T/252V/281P, 24M/237Q/287L/344S, 24M/252V, 24M/252V/287L, 24M/344W, 213S/252V/278L/344S, 252V/278L/344W, 252V/287L/344S, 및 281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 F24M/S168T/M252V/G281P, F24M/A237Q/F287L/H344S, F24M/M252V, F24M/M252V/F287L, F24M/H344W, P213S/M252V/Y278L/H344S, M252V/Y278L/H344W, M252V/F287L/H344S, 및 G281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24, 24/38/49/70/149/161/174/175/189/193/225/231/243/252/271/287/292/293/296/303/334/344/373/385, 38, 49, 70, 149, 161, 174, 175, 189, 193, 225, 231, 243, 252, 271, 287, 292, 293, 296, 303, 334, 344, 373, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24A, 24C, 24D, 24E, 24F, 24F/38F/49V/70K/149T/161L/174E/175Q/189S/193Q/225M/231Q/243L/252M/271G/287F/292M/293Q/296S/303A/334D/344S/373Y/385F, 24G, 24H, 24I, 24K, 24L, 24N, 24P, 24Q, 24R, 24S, 24T, 24V, 24W, 24Y, 38A, 38C, 38D, 38E, 38F, 38H, 38I, 38K, 38L, 38M, 38N, 38P, 38Q, 38R, 38S, 38T, 38V, 38W, 38Y, 49A, 49C, 49D, 49E, 49F, 49G, 49H, 49I, 49K, 49L, 49M, 49N, 49P, 49Q, 49R, 49S, 49V, 49W, 49Y, 70A, 70C, 70D, 70E, 70F, 70G, 70I, 70K, 70L, 70M, 70N, 70P, 70Q, 70R, 70S, 70T, 70V, 70W, 70Y, 149A, 149C, 149D, 149F, 149G, 149H, 149I, 149K, 149L, 149M, 149N, 149P, 149Q, 149R, 149S, 149T, 149V, 149W, 149Y, 161A, 161C, 161D, 161E, 161F, 161G, 161H, 161K, 161L, 161M, 161N, 161P, 161Q, 161R, 161S, 161T, 161V, 161W, 161Y, 174A, 174C, 174D, 174E, 174F, 174G, 174H, 174I, 174K, 174L, 174M, 174N, 174P, 174Q, 174S, 174T, 174V, 174W, 174Y, 175A, 175C, 175D, 175E, 175F, 175G, 175H, 175I, 175K, 175M, 175N, 175P, 175Q, 175R, 175S, 175T, 175V, 175W, 175Y, 189C, 189D, 189E, 189F, 189G, 189H, 189I, 189K, 189L, 189M, 189N, 189P, 189Q, 189R, 189S, 189T, 189V, 189W, 189Y, 193A, 193C, 193D, 193E, 193F, 193G, 193H, 193I, 193K, 193M, 193N, 193P, 193Q, 193R, 193S, 193T, 193V, 193W, 193Y, 225A, 225C, 225D, 225E, 225F, 225G, 225H, 225I, 225K, 225M, 225N, 225P, 225Q, 225R, 225S, 225T, 225V, 225W, 225Y, 231A, 231C, 231D, 231F, 231G, 231H, 231I, 231K, 231L, 231M, 231N, 231P, 231Q, 231R, 231S, 231T, 231V, 231W, 231Y, 243A, 243C, 243D, 243E, 243F, 243G, 243H, 243I, 243K, 243L, 243M, 243N, 243P, 243Q, 243S, 243T, 243V, 243W, 243Y, 252A, 252C, 252D, 252E, 252F, 252G, 252H, 252I, 252K, 252L, 252M, 252N, 252P, 252Q, 252R, 252S, 252T, 252W, 252Y, 271A, 271C, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 287A, 287C, 287D, 287E, 287F, 287G, 287H, 287I, 287K, 287M, 287N, 287P, 287Q, 287R, 287S, 287T, 287V, 287W, 287Y, 292A, 292C, 292D, 292E, 292F, 292G, 292H, 292I, 292K, 292M, 292N, 292P, 292Q, 292R, 292S, 292T, 292V, 292W, 292Y, 293A, 293C, 293D, 293E, 293F, 293G, 293H, 293I, 293K, 293L, 293M, 293N, 293P, 293Q, 293S, 293T, 293V, 293W, 293Y, 296A, 296C, 296D, 296E, 296F, 296G, 296H, 296I, 296K, 296L, 296M, 296N, 296P, 296Q, 296S, 296T, 296V, 296W, 296Y, 303A, 303C, 303D, 303E, 303F, 303G, 303H, 303I, 303K, 303L, 303M, 303N, 303Q, 303R, 303S, 303T, 303V, 303W, 303Y, 334A, 334C, 334D, 334E, 334F, 334G, 334H, 334I, 334K, 334L, 334M, 334N, 334P, 334Q, 334R, 334S, 334V, 334W, 334Y, 344A, 344C, 344D, 344E, 344F, 344G, 344I, 344K, 344L, 344M, 344N, 344P, 344Q, 344R, 344S, 344T, 344V, 344W, 344Y, 373A, 373C, 373D, 373E, 373G, 373H, 373I, 373K, 373L, 373M, 373N, 373P, 373Q, 373R, 373S, 373T, 373V, 373W, 373Y, 385A, 385C, 385D, 385E, 385F, 385G, 385H, 385I, 385K, 385L, 385M, 385N, 385Q, 385R, 385S, 385T, 385V, 385W, 및 385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 M24A, M24C, M24D, M24E, M24F, M24F/G38F/T49V/H70K/E149T/I161L/R174E/L175Q/A189S/L193Q/L225M/E231Q/R243L/V252M/D271G/L287F/L292M/R293Q/R296S/P303A/T334D/H344S/F373Y/P385F, M24G, M24H, M24I, M24K, M24L, M24N, M24P, M24Q, M24R, M24S, M24T, M24V, M24W, M24Y, G38A, G38C, G38D, G38E, G38F, G38H, G38I, G38K, G38L, G38M, G38N, G38P, G38Q, G38R, G38S, G38T, G38V, G38W, G38Y, T49A, T49C, T49D, T49E, T49F, T49G, T49H, T49I, T49K, T49L, T49M, T49N, T49P, T49Q, T49R, T49S, T49V, T49W, T49Y, H70A, H70C, H70D, H70E, H70F, H70G, H70I, H70K, H70L, H70M, H70N, H70P, H70Q, H70R, H70S, H70T, H70V, H70W, H70Y, E149A, E149C, E149D, E149F, E149G, E149H, E149I, E149K, E149L, E149M, E149N, E149P, E149Q, E149R, E149S, E149T, E149V, E149W, E149Y, I161A, I161C, I161D, I161E, I161F, I161G, I161H, I161K, I161L, I161M, I161N, I161P, I161Q, I161R, I161S, I161T, I161V, I161W, I161Y, R174A, R174C, R174D, R174E, R174F, R174G, R174H, R174I, R174K, R174L, R174M, R174N, R174P, R174Q, R174S, R174T, R174V, R174W, R174Y, L175A, L175C, L175D, L175E, L175F, L175G, L175H, L175I, L175K, L175M, L175N, L175P, L175Q, L175R, L175S, L175T, L175V, L175W, L175Y, A189C, A189D, A189E, A189F, A189G, A189H, A189I, A189K, A189L, A189M, A189N, A189P, A189Q, A189R, A189S, A189T, A189V, A189W, A189Y, L193A, L193C, L193D, L193E, L193F, L193G, L193H, L193I, L193K, L193M, L193N, L193P, L193Q, L193R, L193S, L193T, L193V, L193W, L193Y, L225A, L225C, L225D, L225E, L225F, L225G, L225H, L225I, L225K, L225M, L225N, L225P, L225Q, L225R, L225S, L225T, L225V, L225W, L225Y, E231A, E231C, E231D, E231F, E231G, E231H, E231I, E231K, E231L, E231M, E231N, E231P, E231Q, E231R, E231S, E231T, E231V, E231W, E231Y, R243A, R243C, R243D, R243E, R243F, R243G, R243H, R243I, R243K, R243L, R243M, R243N, R243P, R243Q, R243S, R243T, R243V, R243W, R243Y, V252A, V252C, V252D, V252E, V252F, V252G, V252H, V252I, V252K, V252L, V252M, V252N, V252P, V252Q, V252R, V252S, V252T, V252W, V252Y, D271A, D271C, D271E, D271F, D271G, D271H, D271I, D271K, D271L, D271M, D271N, D271P, D271Q, D271R, D271S, D271T, D271V, D271W, D271Y, L287A, L287C, L287D, L287E, L287F, L287G, L287H, L287I, L287K, L287M, L287N, L287P, L287Q, L287R, L287S, L287T, L287V, L287W, L287Y, L292A, L292C, L292D, L292E, L292F, L292G, L292H, L292I, L292K, L292M, L292N, L292P, L292Q, L292R, L292S, L292T, L292V, L292W, L292Y, R293A, R293C, R293D, R293E, R293F, R293G, R293H, R293I, R293K, R293L, R293M, R293N, R293P, R293Q, R293S, R293T, R293V, R293W, R293Y, R296A, R296C, R296D, R296E, R296F, R296G, R296H, R296I, R296K, R296L, R296M, R296N, R296P, R296Q, R296S, R296T, R296V, R296W, R296Y, P303A, P303C, P303D, P303E, P303F, P303G, P303H, P303I, P303K, P303L, P303M, P303N, P303Q, P303R, P303S, P303T, P303V, P303W, P303Y, T334A, T334C, T334D, T334E, T334F, T334G, T334H, T334I, T334K, T334L, T334M, T334N, T334P, T334Q, T334R, T334S, T334V, T334W, T334Y, H344A, H344C, H344D, H344E, H344F, H344G, H344I, H344K, H344L, H344M, H344N, H344P, H344Q, H344R, H344S, H344T, H344V, H344W, H344Y, F373A, F373C, F373D, F373E, F373G, F373H, F373I, F373K, F373L, F373M, F373N, F373P, F373Q, F373R, F373S, F373T, F373V, F373W, F373Y, P385A, P385C, P385D, P385E, P385F, P385G, P385H, P385I, P385K, P385L, P385M, P385N, P385Q, P385R, P385S, P385T, P385V, P385W, 및 P385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에서 제공되는 바와 같이 적어도 하나의 위치에서 적어도 하나의 돌연변이를 포함하고, 위치는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 14-1796의 짝수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함한다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 14-1796의 짝수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함한다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 14-1796의 짝수 번호 서열에 제시된 적어도 하나의 서열을 포함한다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 더 열안정하다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 더 열안정하다. 일부 실시양태에서, 재조합 리파제는 고온 및/또는 저온에 노출된 후 효소 활성을 유지한다. 일부 추가의 실시양태에서, 재조합 리파제는 참조 서열과 비교하여 고온 및/또는 저온에 노출된 후 더 많은 효소 활성을 유지한다. 일부 실시양태에서, 참조 서열은 야생형 리파제인 반면, 일부 다른 실시양태에서, 참조 서열은 또 다른 재조합 리파제이다.
일부 실시양태에서, 재조합 리파제는 낮은 pH 환경에서 안정하고/하거나 활성인 반면, 다른 실시양태에서, 재조합 리파제는 높은 pH 환경에서 안정하고/하거나 활성이고, 추가의 실시양태에서 재조합 리파제는 중성 pH 환경에서 안정하고/하거나 활성이다. 일부 실시양태에서, 재조합 리파제는 낮은 pH 및 높은 pH 환경에서 안정하고/하거나 활성이고, 일부 추가의 실시양태에서, 리파제는 낮은 pH, 중성 pH 및 높은 pH 환경에서 안정하고/하거나 활성이다. 일부 실시양태에서, 재조합 리파제는 낮은 pH, 높은 pH 및/또는 중성 pH 환경에 노출된 후 효소 활성을 유지한다. 일부 추가의 실시양태에서, 재조합 리파제는 참조 서열과 비교하여 높은 pH, 중성 pH 및/또는 낮은 pH 환경(들)에서 더 안정하고/하거나 활성이다. 일부 실시양태에서, 참조 서열은 야생형 리파제인 반면, 다른 실시양태에서, 참조 서열은 또 다른 조작된 리파제이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 7 미만의 pH에서(즉, 산성 pH 조건 또는 수준 하에서) 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 pH 7 미만의 pH에서(즉, 산성 pH 조건 또는 수준 하에서) 더 안정하고/하거나 활성이다. 일부에서 추가 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 5 미만의 pH에서 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 pH 5에서 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 688보다 pH 5에서 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 pH 3.5에서 더 안정하고/하거나 활성하다. 일부 추가의 실시양태에서, 재조합 리파제는 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 pH 3.5에서 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 pH 3에서 더 안정하고/하거나 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 pH 3에서 더 안정하고/하거나 활성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 단백질분해에 대해 더 내성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 단백질분해에 대해 더 내성이다. 일부 추가의 실시양태에서, 재조합 리파제는 트립신에 의한 단백질분해에 대해 내성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 트립신에 의한 단백질분해에 대해 더 내성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 트립신에 의한 단백질분해에 대해 더 내성이다. 일부 실시양태에서, 재조합 리파제는 키모트립신에 의한 단백질분해에 대해 내성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 키모트립신에 의한 단백질분해에 대해 더 내성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 키모트립신에 의한 단백질분해에 대해 더 내성이다. 일부 추가의 실시양태에서, 재조합 리파제는 펩신, 트립신 및/또는 키모트립신에 의한 단백질분해에 대해 내성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 펩신, 트립신 및/또는 키모트립신에 의한 단백질분해에 대해 더 내성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 펩신, 트립신 및/또는 키모트립신에 의한 단백질분해에 대해 더 내성이다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 적어도 하나의 담즙염의 존재 하에 더 활성이다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 적어도 하나의 담즙염의 존재 하에 더 활성이다. 일부 실시양태에서, 재조합 리파제는 담즙염에 노출된 후 효소 활성을 유지한다. 일부 추가의 실시양태에서, 재조합 리파제는 참조 서열과 비교하여 담즙염에 노출된 후 더 많은 효소 활성을 유지한다. 일부 실시양태에서, 참조 서열은 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제이다. 일부 추가의 실시양태에서, 담즙염은 타우로콜레이트이다.
또 다른 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2의 리파제보다 산성 pH에서 더 안정하고/하거나 활성이고, 더 열안정하고, 단백질분해에 더 내성이 있고/있거나, 적어도 하나의 담즙염의 존재 하에 더 활성이다. 또 다른 일부 실시양태에서, 리파제는 식품 및/또는 음료에서 안정하다. 또 다른 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758 및/또는 868의 리파제보다 산성 pH에서 더 안정하고/하거나 활성이고, 더 열안정하고, 단백질분해에 더 내성이 있고/있거나, 적어도 하나의 담즙염의 존재 하에 더 활성이다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 하나의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 하나 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 2개의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 2개의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 3개의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성으로부터 선택되는 적어도 3개의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 2의 리파제와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성 뿐만 아니라 적어도 하나의 추가의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868와 비교하여 산성 pH에서 개선된 안정성 및/또는 활성, 개선된 열안정성, 단백질분해에 대한 개선된 내성, 및/또는 적어도 하나의 담즙염의 존재 하에 개선된 활성 뿐만 아니라 적어도 하나의 추가의 개선된 특성을 나타낸다. 일부 실시양태에서, 재조합 리파제는 참조 서열과 비교하여 i) 향상된 촉매 활성; ii) 산성 pH에 대한 증가된 내성; iii) pH 3.5에 대한 증가된 내성; iv) pH 3에 대한 증가된 내성; v) 적어도 하나의 프로테아제에 대한 증가된 내성; vi) 적어도 하나의 담즙염에 대한 증가된 내성; vii) 증가된 내열성; 또는 i), ii), iii), iv), v), vi), 및 vii) 중 임의의 것의 조합으로부터 선택되는 적어도 하나의 개선된 특성을 나타낸다. 일부 실시양태에서, 참조 서열은 서열번호 2이고, 일부 대안적인 실시양태에서, 참조 서열은 서열번호 94, 350, 442, 540, 646, 758, 및 868로부터 선택된다. 일부 실시양태에서, 재조합 리파제는 적어도 하나의 참조 서열과 비교하여 i) 향상된 촉매 활성; ii) 산성 pH에 대한 증가된 내성; iii) pH 3.5에 대한 증가된 내성; iv) pH 3에 대한 증가된 내성; v) 적어도 하나의 프로테아제에 대한 증가된 내성; vi) 적어도 하나의 담즙염에 대한 증가된 내성; vii) 증가된 내열성; 또는 i), ii), iii), iv), v), vi), 및 vii) 중 임의의 것의 조합으로부터 선택되는 적어도 하나의 개선된 특성을 나타낸다. 일부 실시양태에서, 참조 서열은 서열번호 2이고, 일부 대안적인 실시양태에서, 참조 서열은 서열번호 94, 350, 442, 540, 646, 758, 및 868로부터 선택된다. 일부 실시양태에서, 재조합 리파제는 적어도 2개 이상의 참조 서열과 비교하여 i) 향상된 촉매 활성; ii) 산성 pH에 대한 증가된 내성; iii) pH 3.5에 대한 증가된 내성; iv) pH 3에 대한 증가된 내성; v) 적어도 하나의 프로테아제에 대한 증가된 내성; vi) 적어도 하나의 담즙염에 대한 증가된 내성; vii) 증가된 내열성; 또는 i), ii), iii), iv), v), vi), 및 vii) 중 임의의 것의 조합으로부터 선택되는 적어도 하나의 개선된 특성을 나타낸다. 일부 실시양태에서, 프로테아제는 펩신, 트립신, 및/또는 키모트립신으로부터 선택된다. 일부 실시양태에서, 참조 서열은 서열번호 2, 94, 350, 442, 540, 646, 758, 및 868로부터 선택된다. 일부 추가의 실시양태에서, 재조합 리파제는 정제된다.
본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 코딩하는 재조합 폴리뉴클레오티드 서열을 제공한다. 일부 실시양태에서, 재조합 폴리뉴클레오티드 서열은 코돈 최적화된다. 일부 추가의 실시양태에서, 재조합 폴리뉴클레오티드는 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99%, 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오티드는 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 본 발명은 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열을 제공한다. 일부 추가의 실시양태에서, 재조합 폴리뉴클레오티드 서열은 서열번호 13-1795의 홀수 번호 서열로부터 선택되는 서열을 포함한다. 일부 실시양태에서, 재조합 폴리뉴클레오티드 서열은 서열번호 13-1795의 홀수 번호 서열로부터 선택되는 서열을 포함하고, 상기 서열은 본원에 제공된 재조합 폴리펩티드를 코딩한다. 일부 실시양태에서, 재조합 폴리뉴클레오티드 서열은 서열번호 13-1795의 홀수 번호 서열로부터 선택되는 서열을 포함하고, 상기 서열은 서열번호 14-1796에 제공된 짝수 번호 서열에 제공된 재조합 폴리펩티드를 코딩한다. 일부 추가의 실시양태에서, 본원에 제공된 재조합 리파제를 코딩하는 재조합 폴리뉴클레오티드는 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99%, 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 본원에 제공된 재조합 리파제를 코딩하는 재조합 폴리뉴클레오티드는 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 본원에 제공된 재조합 리파제를 코딩하는 재조합 폴리뉴클레오티드는 서열번호 13-1795의 홀수 번호 서열에 제시된 적어도 하나의 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 서열을 포함하고, 재조합 리파제는 서열번호 14-1796에 제공된 짝수 번호 서열을 포함하는 폴리펩티드 서열을 포함한다.
본 발명은 또한 본원에 제공된 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 발현 벡터를 제공한다. 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 코딩하는 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 발현 벡터를 제공한다. 일부 추가의 실시양태에서, 재조합 폴리뉴클레오티드 서열은 제어 서열에 작동 가능하게 연결된다. 일부 실시양태에서, 제어 서열은 프로모터이다. 일부 추가의 실시양태에서, 프로모터는 이종 프로모터이다.
본 발명은 또한 본원에 제공된 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 제공한다. 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 코딩하는 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 제공한다. 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 코딩하는 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 발현 벡터를 포함하는 숙주 세포를 제공한다. 본 발명은 또한 본원에 제공된 재조합 리파제를 코딩하는 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 발현 벡터를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 숙주 세포는 진핵성인 반면, 일부 대안적인 실시양태에서, 숙주 세포는 원핵성이다. 일부 실시양태에서, 숙주 세포는 에쉐리키아 콜라이(Escherichia coli)이다. 일부 대안적인 실시양태에서, 숙주 세포는 사카로마이세스 세레비지애(Saccharomyces cerevisiae)이다.
본 발명은 또한 재조합 폴리뉴클레오티드에 의해 코딩되는 재조합 리파제가 생성되는 조건 하에 본원에 제공된 하나 이상의 숙주 세포를 배양하는 단계를 포함하는, 적어도 하나의 재조합 리파제의 제조 방법을 제공한다. 일부 실시양태에서, 방법은 리파제를 회수하는 단계를 추가로 포함한다. 또 다른 일부 추가의 실시양태에서, 방법은 리파제를 정제하는 단계를 추가로 포함한다.
본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 포함하는 조성물을 제공한다. 일부 실시양태에서, 적어도 하나의 재조합 리파제를 포함하는 조성물은 약학적 조성물을 포함한다. 일부 추가의 실시양태에서, 약학적 조성물은 췌장 기능부전의 치료에 적합하다. 일부 추가의 실시양태에서, 약학적 조성물은 약학적으로 허용되는 담체 및/또는 부형제를 추가로 포함한다. 일부 실시양태에서, 약학적 조성물은 인간에 대한 비경구 주사 또는 주입에 적합하다. 일부 추가의 실시양태에서, 약학적 조성물은 인간에 대한 경구 투여에 적합하다. 일부 추가의 실시양태에서, 약학적 조성물은 인간에 대한 다른 투여 방식에 적합하다. 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 포함하는 조성물을 제공하며, 조성물은 다른 용도에 적합하다.
본 발명은 또한 췌장 기능부전을 갖는 대상체를 제공하는 단계, 및 본원에 제공된 약학적 조성물을 대상체에게 제공하는 단계를 포함하는, 대상체에서 췌장 기능부전의 증상을 치료 및/또는 예방하는 방법을 제공한다. 일부 실시양태에서, 췌장 기능부전의 증상은 대상체에게 적어도 하나의 재조합 리파제를 포함하는 조성물을 투여할 때 대상체에서 개선된다. 일부 추가의 실시양태에서, 약학적 조성물은 적어도 하나의 프로테아제를 추가로 포함한다. 일부 추가의 실시양태에서, 약학적 조성물은 적어도 하나의 아밀라제를 추가로 포함한다. 또 다른 일부 추가의 실시양태에서, 약학적 조성물은 적어도 하나의 프로테아제 및 적어도 하나의 아밀라아제를 추가로 포함한다.
일부 추가의 실시양태에서, 대상체는 췌장 기능부전의 증상을 나타내는 대상체가 필요로 하는 식이보다 지질 함량이 덜 제한되는 식이를 먹을 수 있다. 일부 추가의 실시양태에서, 대상체는 유아인 반면, 일부 다른 실시양태에서, 대상체는 소아이다. 일부 추가의 실시양태에서, 대상체는 성인인 반면, 일부 대안적인 실시양태에서, 대상체는 젊은 성인이다. 본 발명은 추가로 본원에 제공된 적어도 하나의 재조합 리파제를 포함하는 의약을 제공한다.
일부 실시양태에서, 본 발명은 적어도 하나의 지방 및/또는 지질 및 본 발명의 적어도 하나의 조작된 리파제를 제공하는 단계, 및 지방 및/또는 지질이 분해되는 조건 하에 지방 및/또는 지질을 적어도 하나의 조작된 아밀라제에 노출시키는 단계를 포함하는, 지방 및/또는 지질을 분해하는 방법을 제공한다. 일부 추가의 실시양태에서, 본 발명은 적어도 하나의 지방 및/또는 지질 및 본 발명의 적어도 하나의 조작된 리파제를 포함하는 조성물을 제공하는 단계, 및 지방 및/또는 지질이 분해되는 조건 하에 지방 및/또는 지질을 적어도 하나의 조작된 아밀라제를 포함하는 조성물에 노출시키는 단계를 포함하는, 지방 및/또는 지질을 분해하는 방법을 제공한다. 본 발명은 또한 본원에 제공된 적어도 하나의 재조합 리파제를 포함하는 조성물의 용도를 제공한다. 본 발명은 지방 및/또는 지질 및 본원에 제공된 적어도 하나의 조작된 리파제를 제공하는 단계; 및 상기 지방 및/또는 지질이 상기 조작된 아밀라제에 의해 가수분해되도록 하는 조건 하에 상기 지방 및/또는 지질을 상기 조작된 리파제에 노출시키는 단계를 포함하는, 지방 및/또는 지질을 가수분해하는 방법을 제공한다. 일부 추가의 실시양태에서, 본 발명은 지방 및/또는 지질 및 본원에 제공된 적어도 하나의 조작된 리파제를 포함하는 조성물을 제공하는 단계; 및 상기 지방 및/또는 지질이 상기 조성물의 상기 조작된 리파제에 의해 가수분해되도록 하는 조건 하에 상기 지방 및/또는 지질을 상기 조성물에 노출시키는 단계를 포함하는, 지방 및/또는 지질을 가수분해하는 방법을 제공한다.
발명의 설명
본 발명은 조작된 리파제 폴리펩티드 및 이의 조성물을 제공한다. 조작된 리파제 폴리펩티드는 개선된 열안정성, 프로테아제 안정성, 및 산성(pH <7) 조건을 포함한 다양한 pH 조건에서 안정성을 제공하도록 최적화되었다. 본 발명은 또한 치료 목적을 위한 조작된 리파제 폴리펩티드를 포함하는 조성물의 용도에 관한 것이다. 일부 실시양태에서, 본 발명의 리파제 변이체는 PEI 병태의 PERT 치료에 사용된다. 일부 추가의 실시양태에서, 리파제는 장용 코팅 및/또는 양성자 펌프 억제제(PPI)를 필요로 하지 않는 포맷으로 투여된다. 본 발명은 또한 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 뿐만 아니라 조작된 폴리뉴클레오티드 및 리파제 폴리펩티드의 제조 방법을 제공한다.
약어 및 정의:
달리 정의되지 않는 한, 본원에서 사용되는 모든 기술 및 과학 용어는 일반적으로 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자에 의해 일반적으로 이해되는 것과 동일한 의미를 갖는다. 일반적으로, 본원에 사용된 명명법 및 하기에 기재된 세포 배양, 분자 유전학, 미생물학, 생화학, 유기 화학, 분석 화학 및 핵산 화학의 실험실 절차는 당업계에서 널리 공지되어 있고 통상적으로 사용되는 것들이다. 이러한 기술은 널리 공지되어 있고, 당업자에게 널리 공지된 수많은 텍스트 및 참조 문헌에 설명되어 있다. 화학 합성 및 화학 분석을 위해 표준 기술 또는 이의 변형이 이용된다. 상기 및 하기에서 본원에서 언급되는 모든 특허, 특허 출원, 논문 및 공개문은 본원에 참조로 명시적으로 포함된다.
본원에 기재된 것과 유사하거나 동등한 임의의 적합한 방법 및 재료가 본 발명의 실시에 사용되지만, 일부 방법 및 재료가 본원에 기재되어 있다. 본 발명은 기술된 특정 방법론, 프로토콜 및 시약으로 제한되지 않는 것으로 이해되어야 하며, 이는 이들이 당업자에 의해 사용되는 맥락에 따라 달라질 수 있기 때문이다. 따라서, 바로 하기에서 정의되는 용어들은 전체로서 본 출원을 참조하여 보다 완전하게 설명된다. 상기 및 하기에서 언급되는 모든 특허, 특허 출원, 논문 및 공개문은 본원에 참조로 명시적으로 포함된다.
본원에서 사용되는 바와 같이, 단수 형태는 문맥이 명백하게 달리 나타내지 않는 한 복수 참조를 포함한다.
수치 범위는 범위를 정의하는 숫자를 포함한다. 따라서, 본원에 개시된 모든 수치 범위는 마치 이러한 보다 좁은 수치 범위가 모두 본원에 명시적으로 기재된 것처럼 이러한 보다 넓은 수치 범위 내에 속하는 모든 보다 좁은 수치 범위를 포괄하도록 의도된다. 또한, 본원에 개시된 모든 최대 (또는 최소) 수치 제한은 마치 이러한 더 낮은 (또는 더 높은) 수치 제한이 본원에 명시적으로 기재된 것처럼 모든 더 낮은 (또는 더 높은) 수치 제한을 포함하도록 의도된다.
용어 "약"은 특정 값에 대해 허용 가능한 오차를 의미한다. 일부 경우에, "약"은 주어진 값 범위의 0.05%, 0.5%, 1.0% 또는 2.0% 이내를 의미한다. 일부 경우에, "약"은 주어진 값의 1, 2, 3 또는 4 표준 편차 이내를 의미한다.
더욱이, 본원에 제공된 표제는 전체로서 본 출원을 참조하여 가질 수 있는 본 발명의 다양한 측면 또는 실시양태의 제한이 아니다. 따라서, 바로 하기에서 정의되는 용어들은 전체로서 본 출원을 참조하여 보다 완전하게 정의된다. 그럼에도 불구하고, 본 발명의 이해를 용이하게 하기 위해, 다수의 용어가 하기에 정의된다.
달리 표시되지 않는 한, 핵산은 5'에서 3' 방향으로 왼쪽에서 오른쪽으로 작성된다. 아미노산 서열은 각각 아미노에서 카르복시 방향으로 왼쪽에서 오른쪽으로 작성된다.
본원에서 사용되는 바와 같이, 용어 "포함하는" 및 이의 동족어는 포괄적인 의미(즉, 용어 "포함하는" 및 이의 상응하는 동족어와 동등)로 사용된다.
본원에서 사용되는 바와 같이, "EC" 번호는 국제 생화학 및 분자 생물학 연합의 명명 위원회(NC-IUBMB)의 효소 명명법을 지칭한다. IUBMB 생화학적 분류는 효소가 촉매하는 화학 반응을 기반으로 하는 효소의 수치적 분류 시스템이다.
본원에서 사용되는 바와 같이, "ATCC"는 생물저장소 컬렉션이 유전자 및 균주를 포함하는 아메리칸 타입 컬쳐 컬렉션을 지칭한다.
본원에서 사용되는 바와 같이, "NCBI"는 국립 생물학 정보 센터 및 본원에 제공된 서열 데이터베이스를 지칭한다.
본원에서 사용되는 바와 같이, "리파제"는 트리글리세라이드의 에스테르 결합을 가수분해함으로써 지방의 가수분해를 촉매하는, 일반적으로 "리파제"로 지칭되는 임의의 효소를 지칭한다. 췌장 리파제는 지방을 지방산, 글리세롤 및 다른 알콜로 분해하는 데 중요하다. 리파제는 대부분의 유기체에서 식이 지질의 소화, 수송 및 처리에 필수적이다.
본원에서 사용되는 바와 같이, 용어 "지질"은 지방산 및 이들의 에스테르, 스테롤, 프레놀, 특정 난용성 비타민, 및 다른 관련 화합물을 포함하는 수불용성 거대분자의 부류를 지칭한다. "지방"은 지방산 에스테르(예컨대, 글리세롤 및 3개의 지방산으로 제조된 트리글리세라이드)로 구성된 지질의 서브세트이다. 본 발명은 임의의 특정 지질 및/또는 지방으로 제한되는 것으로 의도되지 않는다. 문맥을 고려하여, 용어 "지방" 및 "지질"은 본원에서 상호교환적으로 사용된다.
본원에서 사용되는 바와 같이, "프로테아제" (및 "프로테이나제" 및 "펩티다제")는 단백질을 가수분해하는 수많은 효소를 지칭한다. 단백질을 더 작은 폴리펩티드 언타이 또는 단일 아미노산으로 분해하는 데 관여하는 수많은 프로테아제가 있다. 프로테아제는 섭취된 단백질의 소화, 단백질 이화작용 및 세포 신호전달을 포함한 수많은 생물학적 기능에 중요하다.
"단백질", "폴리펩티드" 및 "펩티드"는 길이 또는 번역후 변형(예컨대, 글리코실화 또는 포스포릴화)에 관계없이 아미드 결합에 의해 공유 연결된 적어도 2개의 아미노산의 중합체를 나타내기 위해 본원에서 상호교환적으로 사용된다.
"아미노산"은 본원에서 일반적으로 알려진 3문자 기호 또는 IUPAC-IUB 생화학적 명명법 위원회에 의해 권장되는 1문자 기호로 지칭된다. 유사하게, 뉴클레오티드는 일반적으로 허용되는 단일 문자 코드로 지칭될 수 있다.
용어 "조작된", "재조합", "비-자연 발생" 및 "변이체"는, 세포, 폴리뉴클레오티드 또는 폴리펩티드와 관련하여 사용되는 경우, 달리 자연에 존재하지 않거나 동일하지만 합성 물질 및/또는 재조합 기술을 이용한 조작에 의해 생성 또는 유도되는 방식으로 변형된 물질 또는 물질의 천연 또는 고유 형태에 상응하는 물질을 지칭한다.
재조합 폴리펩티드는 당업계에 공지된 임의의 적합한 방법을 이용하여 제조될 수 있다. 관심의 야생형 폴리펩티드를 코딩하는 유전자는 플라스미드와 같은 벡터에 클로닝될 수 있고, 이. 콜라이(E. coli), 에스. 세레비지애(S. cerevisiae) 등과 같은 원하는 숙주에서 발현될 수 있다. 재조합 폴리펩티드의 변이체는 당업계에 공지된 다양한 방법에 의해 제조될 수 있다. 실제로, 당업자에게 널리 공지된 다양한 상이한 돌연변이유발 기술이 존재한다. 또한, 돌연변이유발 키트는 또한 많은 상업적 분자 생물학 공급업체로부터 입수될 수 있다. 정의된 아미노산에서 특정 치환(부위 지정), 유전자의 국소 영역에서 특정 또는 무작위 돌연변이(지역 특정), 또는 전체 유전자에 대한 무작위 돌연변이유발(예컨대, 포화 돌연변이유발)을 일으키는 방법이 이용 가능하다. PCR, 카세트 돌연변이유발, 유전자 합성, 오류가 발생하기 쉬운 PCR(error-prone PCR), 셔플링, 및 화학적 포화 돌연변이유발, 또는 당업계에 공지된 임의의 다른 적합한 방법을 포함하지만 이에 제한되지 않는 많은 적합한 방법이 효소 변이체를 생성하기 위해 당업자에게 공지되어 있다. DNA 및 단백질 조작에 사용되는 방법의 비제한적 예는 하기 특허에서 제공된다: 미국 특허 번호 6,117,679; 미국 특허 번호 6,420,175; 미국 특허 번호 6,376,246; 미국 특허 번호 6,586,182; 미국 특허 번호 7,747,391; 미국 특허 번호 7,747,393; 미국 특허 번호 7,783,428; 및 미국 특허 번호 8,383,346. 변이체가 생성된 후, 원하는 특성(예컨대, 높거나 증가된 활성, 또는 낮거나 감소된 활성, 증가된 열 활성, 증가된 열 안정성, 및/또는 산성 pH 안정성 등)에 대해 스크리닝될 수 있다. 일부 실시양태에서, "재조합 리파제 폴리펩티드"(본원에서 "조작된 리파제 폴리펩티드", "변이체 리파제 효소" 및 "리파제 변이체"로도 지칭됨)가 사용된다.
본원에서 사용되는 바와 같이, "야생형" 및 "자연 발생"은 자연에서 발견되는 형태를 지칭한다. 예컨대, 야생형 폴리펩티드 또는 폴리뉴클레오티드 서열은 자연에서 공급원으로부터 단리될 수 있고 인간 조작에 의해 의도적으로 변형되지 않은 유기체에 존재하는 서열이다.
본원에서 사용되는 바와 같이, "코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예컨대, 유전자)의 부분을 지칭한다.
용어 "서열 동일성 백분율(%)"은 폴리뉴클레오티드 및 폴리펩티드 간의 비교를 지칭하기 위해 본원에 사용되며, 비교 창에서 2개의 최적으로 정렬된 서열을 비교함으로써 결정되고, 비교 창에서 폴리뉴클레오티드 또는 폴리펩티드 서열의 부분은 2개의 서열의 최적 정렬을 위해 참조 서열과 비교하여 추가 또는 결실(즉, 갭)을 포함할 수 있다. 백분율은 일치된 위치의 수를 산출하기 위해 두 서열에서 동일한 핵산 염기 또는 아미노산 잔기가 발생하는 위치의 수를 결정하고, 일치된 위치의 수를 비교 창의 총 위치 수로 나누고, 결과에 100을 곱하여 서열 동일성 백분율을 산출함으로서 계산될 수 있다. 대안적으로, 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열 모두에서 발생하거나 핵산 염기 또는 아미노산 잔기가 갭과 함께 정렬되어 일치하는 위치의 수를 산출하는 위치의 수를 결정하고, 일치된 위치의 수를 비교 창의 총 위치 수로 나누고, 결과에 100을 곱하여 서열 동일성 백분율을 산출함으로써 계산될 수 있다. 당업자는 2개의 서열을 정렬하는 데 이용 가능한 많은 확립된 알고리즘이 있음을 인식한다. 비교를 위한 서열의 최적 정렬은 당업계에 공지된 바와 같이, 예컨대 문헌(Smith and Waterman, Adv. Appl. Math., 2:482 [1981])의 국소 상동성 알고리즘에 의해, 문헌(Needleman and Wunsch, J. Mol. Biol., 48:443 [1970])의 상동성 정렬 알고리즘에 의해, 문헌(Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988])의 유사성 방법 검색에 의해, 이러한 알고리즘의 컴퓨터화된 구현(예컨대, GCG 위스콘신 소프트웨어 패키지의 GAP, BESTFIT, FASTA 및 TFASTA)에 의해, 또는 육안 검사에 의해 수행될 수 있다. 서열 동일성 및 서열 유사성 백분율을 결정하기에 적합한 알고리즘의 예는 알트슐(Altschul) 등에 의해 설명되는 바와 같은(각각 Altschul et al., J. Mol. Biol., 215: 403-410 [1990] 및 Altschul et al., Nucleic Acids Res., 3389-3402 [1977] 참조) BLAST 및 BLAST 2.0 알고리즘을 포함하지만 이에 제한되지 않는다. BLAST 분석을 수행하기 위한 소프트웨어는 국립 생명공학 정보 센터 웹사이트를 통해 공개적으로 이용 가능하다. 이 알고리즘은 먼저 데이터베이스 서열에서 동일한 길이의 단어와 정렬될 때 일부 양의 값 임계값 점수 T와 일치하거나 이를 충족하는 쿼리 서열에서 길이 W의 짧은 단어를 확인하여 고득점 서열 쌍(HSP)을 확인하는 것을 수반한다. T는 이웃 단어 점수 임계값으로 지칭된다(Altschul et al., 상기 참조). 이러한 초기 이웃 단어 히트는 검색을 시작하여 이를 함유하는 더 긴 HSP를 찾기 위한 시드 역할을 한다. 이어서, 누적 정렬 점수가 증가될 수 있는 한, 단어 히트는 각각의 서열을 따라 양방향으로 확장된다. 누적 점수는 뉴클레오티드 서열의 경우 파라미터 M(일치하는 잔기 쌍에 대한 보상 점수, 항상 >0) 및 N(일치하지 않는 잔기에 대한 벌점 점수, 항상 <0)을 사용하여 계산된다. 아미노산 서열의 경우, 득점 매트릭스를 사용하여 누적 점수를 계산한다. 하기의 경우, 각각의 방향으로의 단어 히트의 확장이 중단된다: 누적 정렬 점수가 최대 달성 값으로부터 수량 X만큼 떨어질 때; 하나 이상의 음의 득점 잔기 정렬의 누적으로 인해 누적 점수가 0 미만이 될 때; 또는 서열의 말단에 도달될 때. BLAST 알고리즘 파라미터 W, T 및 X는 정렬의 민감도 및 속도를 결정한다. BLASTN 프로그램(뉴클레오티드 서열용)은 디폴트로서 단어길이(W) 11, 기대값(E) 10, M=5, N=-4 및 두 가닥의 비교를 사용한다. 아미노산 서열의 경우, BLASTP 프로그램은 디폴트로서 단어길이(W) 3, 기대값(E) 10, 및 BLOSUM62 득점 매트릭스를 사용한다(Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]). 서열 정렬 및 서열 동일성 %의 예시적인 결정은 제공된 디폴트 파라미터를 사용하여 GCG 위스콘신 소프트웨어 패키지(Accelrys, Madison WI)의 BESTFIT 또는 GAP 프로그램을 사용할 수 있다.
본원에서 사용되는 바와 같이, "참조 서열"은 서열 비교를 위한 기초로 사용되는 정의된 서열을 지칭한다. 참조 서열은 큰 서열의 서브세트, 예컨대 전장 유전자 또는 폴리펩티드 서열의 분절일 수 있다. 일반적으로, 참조 서열은 적어도 20개의 뉴클레오티드 또는 아미노산 잔기 길이, 적어도 25개의 잔기 길이, 적어도 50개의 잔기 길이, 적어도 100개의 잔기 길이 또는 핵산 또는 폴리펩티드의 전장이다. 2개의 폴리뉴클레오티드 또는 폴리펩티드는 각각 (1) 2개의 서열 간에 유사한 서열(즉, 완전한 서열의 일부)을 포함할 수 있고, (2) 2개의 서열 간에 상이한 서열을 추가로 포함할 수 있기 때문에, 2개 (또는 그 초과)의 폴리뉴클레오티드 또는 폴리펩티드 사이의 서열 비교는 전형적으로 "비교 창"을 통해 2개의 폴리뉴클레오티드 또는 폴리펩티드의 서열을 비교하여 서열 유사성의 국부적 영역을 확인하고 비교함으로써 수행된다. 일부 실시양태에서, "참조 서열"은 1차 아미노산 서열에 기초할 수 있고, 참조 서열은 1차 서열에서 하나 이상의 변화를 가질 수 있는 서열이다. "비교 창"은 적어도 약 20개의 인접 뉴클레오티드 위치 또는 아미노산 잔기의 개념적 분절을 지칭하고, 서열은 적어도 20개의 인접 뉴클레오티드 또는 아미노산의 참조 서열과 비교될 수 있고, 비교 창에서 서열의 일부는 2개의 서열의 최적 정렬을 위해 (추가 또는 결실을 포함하지 않는) 참조 서열과 비교하여 20% 이하의 추가 또는 결실(즉, 갭)을 포함할 수 있다. 비교 창은 20개의 인접 잔기보다 길 수 있으며, 임의적으로 30, 40, 50, 100 또는 더 긴 창을 포함한다.
주어진 아미노산 또는 폴리뉴클레오티드 서열의 번호매김과 관련하여 사용될 때의 "에 상응하는", "을 참조하여" 또는 "와 비교하여"는, 주어진 아미노산 또는 폴리뉴클레오티드 서열이 참조 서열과 비교될 때 특정 참조 서열의 잔기의 번호매김을 지칭한다. 다시 말해서, 주어진 중합체의 잔기 번호 또는 잔기 위치는 주어진 아미노산 또는 폴리뉴클레오티드 서열 내의 잔기의 실제 수치 위치보다는 참조 서열과 관련하여 지정된다. 예컨대, 조작된 리파제의 아미노산 서열과 같은 주어진 아미노산 서열은 두 서열 사이의 잔기 일치를 최적화하기 위해 갭을 도입함으로써 참조 서열에 대해 정렬될 수 있다. 이러한 경우에, 갭이 존재하지만, 주어진 아미노산 또는 폴리뉴클레오티드 서열의 잔기의 번호매김은 이것이 정렬된 참조 서열과 관련하여 이루어진다.
본원에서 사용되는 바와 같이, "돌연변이"는 폴리펩티드 또는 폴리뉴클레오티드 서열의 임의의 변화를 지칭한다. 이는 서열에 존재하는(즉, 시작 또는 참조 서열과 비교하여) 임의의 수(즉, 하나 이상)의 치환, 삽입, 결실 및/또는 재배열을 포괄하도록 의도된다. 따라서, 서열의 돌연변이는 본원에서 제공되는 바와 같이 변이체 폴리펩티드(예컨대, 변이체 또는 재조합 리파제)의 생성을 초래한다.
본원에서 사용되는 바와 같이, "아미노산 차이" 또는 "잔기 차이"는 참조 서열의 상응하는 위치에 있는 아미노산 잔기와 비교하여 폴리펩티드 서열의 임의의 위치에 있는 아미노산 잔기의 차이를 지칭한다. 아미노산 차이의 위치는 일반적으로 본원에서 "Xn"으로 지칭되며, n은 잔기 차이의 기초가 되는 참조 서열의 상응하는 위치를 지칭한다. 예컨대, "서열번호 2와 비교하여 위치 X92에서의 잔기 차이"는 서열번호 2의 위치 92에 상응하는 폴리펩티드 위치에서의 아미노산 잔기의 차이를 지칭한다. 따라서, 서열번호 2의 참조 폴리펩티드가 위치 92에 트레오닌을 갖는 경우, "서열번호 2와 비교하여 위치 X92에서의 잔기 차이"는 서열번호 2의 위치 92에 상응하는 폴리펩티드의 위치에 트레오닌 이외의 아미노산 잔기가 있다는 것을 의미한다(예컨대, T92A). 본원의 대부분의 경우에서, 한 위치에서 특정 아미노산 잔기 차이는 "XnY"로 표시되고, "Xn"은 상기 기재된 바와 같이 상응하는 위치를 특정하고 "Y"는 조작된 폴리펩티드에서 발견되는 아미노산(즉, 참조 폴리펩티드와 상이한 잔기)의 단일 문자 식별자이다. 일부 예에서(예컨대, 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7 및/또는 5-1에서), 본 개시내용은 또한 기존 표기법 "AnB"로 표시되는 특정 아미노산 차이를 제공하고, A는 참조 서열에서 잔기의 단일 문자 식별자이고, "n"은 참조 서열에서 잔기 위치의 번호이고, B는 조작된 폴리펩티드의 서열에서 잔기 치환의 단일 문자 식별자이다. 일부 예에서, 본 개시내용의 폴리펩티드는 참조 서열과 비교하여 하나 이상의 아미노산 잔기 차이를 포함할 수 있고, 이는 참조 서열과 비교하여 잔기 차이가 존재하는 특정 위치의 목록에 의해 표시된다. 일부 실시양태에서, 하나 이상의 아미노산이 폴리펩티드의 특정 잔기 위치에 사용될 수 있는 경우, 사용될 수 있는 다양한 아미노산 잔기는 "/"(예컨대, X2H/X2M/X2T 또는 X2H/M /T 또는 E2H/M/T)로 구분된다. 일부 실시양태에서, 효소 변이체는 1개 초과의 치환을 포함한다. 이러한 치환은 판독이 쉽도록 슬래시로 구분된다(예컨대, Y27V/F385P). 본 출원은 보존적 및 비-보존적 아미노산 치환 중 하나/또는 둘 다를 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩티드 서열을 포함한다.
본원에서 사용되는 바와 같이, "보존적 아미노산 치환"은 유사한 측쇄를 갖는 상이한 잔기로의 잔기의 치환을 지칭하고, 따라서 전형적으로 폴리펩티드 내의 아미노산을 동일하거나 유사한 정의된 부류의 아미노산으로 치환하는 것을 수반한다. 비제한적인 예로서, 지방족 측쇄를 갖는 아미노산은 또 다른 지방족 아미노산(예컨대, 알라닌, 발린, 루이신 및 이소루이신)으로 치환될 수 있고; 히드록실 측쇄를 갖는 아미노산은 히드록실 측쇄를 갖는 또 다른 아미노산(예컨대, 세린 및 트레오닌)으로 치환되고; 방향족 측쇄를 갖는 아미노산은 방향족 측쇄를 갖는 또 다른 아미노산(예컨대, 페닐알라닌, 티로신, 트립토판 및 히스티딘)으로 치환되고; 염기성 측쇄를 갖는 아미노산은 염기성 측쇄를 갖는 또 다른 아미노산(예컨대, 라이신 및 아르기닌)으로 치환되고; 산성 측쇄를 갖는 아미노산은 산성 측쇄를 갖는 또 다른 아미노산(예컨대, 아스파르트산 또는 글루탐산)으로 치환되고; 및/또는 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다.
본원에서 사용되는 바와 같이, "비-보존적 치환"은 상당히 상이한 측쇄 특성을 갖는 아미노산으로 폴리펩티드 내의 아미노산의 치환을 지칭한다. 비-보존적 치환은 정의된 그룹 내부가 아니라 그 사이의 아미노산을 사용할 수 있으며, (a) 치환 영역에서 펩티드 골격의 구조(예컨대, 글리신 대신 프롤린), (b) 전하 또는 소수성, 또는 (c) 측쇄의 크기에 영향을 미친다. 비제한적인 예로서, 예시적인 비-보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산; 작은 아미노산으로 치환된 방향족 아미노산; 및 소수성 아미노산으로 치환된 친수성 아미노산일 수 있다.
본원에서 사용되는 바와 같이, "결실"은 참조 폴리펩티드로부터 하나 이상의 아미노산을 제거하는 것에 의한 폴리펩티드에 대한 변형을 지칭한다. 결실은, 효소 활성을 유지하고/하거나 조작된 효소의 개선된 특성을 유지하면서 참조 효소를 구성하는 아미노산의 총 수의 최대 10%까지 또는 최대 20%까지, 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산, 또는 20개 이상의 아미노산의 제거를 포함할 수 있다. 결실은 폴리펩티드의 내부 부분 및/또는 말단 부분으로 지시될 수 있다. 다양한 실시양태에서, 결실은 연속적인 분절을 포함할 수 있거나 불연속적일 수 있다.
본원에서 사용되는 바와 같이, "삽입"은 참조 폴리펩티드로부터 하나 이상의 아미노산의 부가에 의한 폴리펩티드의 변형을 지칭한다. 삽입은 폴리펩티드의 내부 부분, 또는 카르복시 또는 아미노 말단에 있을 수 있다. 본원에 사용된 삽입물은 당업계에 공지된 융합 단백질을 포함한다. 삽입은 아미노산의 인접한 분절일 수 있거나 자연 발생 폴리펩티드에서 하나 이상의 아미노산에 의해 분리될 수 있다.
본원에서 사용되는 바와 같이, 폴리뉴클레오티드 서열과 관련하여 사용된 별표(*)는 폴리뉴클레오티드 서열 내 정지 코돈의 존재를 나타낸다. 일부 실시양태에서, 변이체 프로테아제는 정지 코돈의 존재로 인해 시작 또는 참조 서열과 비교하여 말단절단된다.
"기능적 단편" 및 "생물학적 활성 단편"은 본원에서 상호교환적으로 사용되고, 아미노 말단 및/또는 카르복시 말단 결실(들) 및/또는 내부 결실을 갖지만(예컨대, 서열이 말단절단되지만), 나머지 아미노산 서열은 비교되고 있는 서열(예컨대, 본 발명의 전장의 조작된 리파제)의 상응하는 위치와 동일하고 전장 폴리펩티드의 모든 활성을 실질적으로 유지하는 폴리펩티드를 지칭한다.
본원에서 사용되는 바와 같이, "단리된 폴리펩티드"는 자연적으로 이를 수반하는 다른 오염물(예컨대, 단백질, 지질 및 폴리뉴클레오티드)로부터 실질적으로 분리된 폴리펩티드를 지칭한다. 이 용어는 자연 발생 환경 또는 발현 시스템(예컨대, 숙주 세포 또는 시험관내 합성)으로부터 제거되거나 정제된 폴리펩티드를 포괄한다. 재조합 리파제 폴리펩티드는 세포 내에 존재하거나, 세포 배지에 존재하거나, 용해물 또는 단리된 제제와 같은 다양한 형태로 제조될 수 있다. 이와 같이, 일부 실시양태에서, 재조합 리파제 폴리펩티드는 단리된 폴리펩티드일 수 있다.
용어 "단리된" 및 "정제된"은 분자(예컨대, 단리된 핵산, 폴리펩티드 등) 또는 자연적으로 회합된 적어도 하나의 다른 성분으로부터 제거되는 다른 성분을 지칭하기 위해 사용된다. 용어 "정제된"은 절대적인 순도를 요구하는 것이 아니라 상대적인 정의로 의도된다.
본원에서 사용되는 바와 같이, "실질적으로 순수한 폴리펩티드"는 폴리펩티드 종이 존재하는 우세한 종인 조성물을 지칭하고(즉, 몰 또는 중량 기준으로 조성물 내의 임의의 다른 개별 거대분자 종보다 더 풍부함), 대상 종이 몰 또는 중량%로 존재하는 거대분자 종의 적어도 약 50%를 포함할 때 일반적으로 실질적으로 정제된 조성물이다. 일반적으로, 실질적으로 순수한 리파제 조성물은 조성물에 존재하는 몰 또는 중량%에 의해 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 95% 이상, 및 약 98% 이상의 모든 거대분자 종을 포함한다. 일부 실시양태에서, 대상 종은 출발 제제로부터 본질적 균질하게 정제되며(즉, 오염 종은 통상적인 검출 방법에 의해 조성물에서 검출될 수 없음), 조성물은 본질적으로 단일 거대분자 종으로 이루어진다. 용매 종, 소분자(<500 달톤) 및 원소 이온 종은 거대분자 종으로 간주되지 않는다. 일부 실시양태에서, 단리된 재조합 리파제 폴리펩티드는 실질적으로 순수한 폴리펩티드 조성물이다. 일부 실시양태에서, 단리된 재조합 리파제 폴리펩티드는 실질적으로 순수한 폴리펩티드 조성물이다. 일부 실시양태에서, 실질적으로 순수한 재조합 리파제 폴리펩티드 제제는 본 발명에서 사용하기에 적합한 제형(예컨대, 다당류, 계면활성제 등)에 첨가된다.
본원에서 사용되는 바와 같이, 용어 "개선된 효소 특성" 및 "개선된 특성"은 참조 리파제 폴리펩티드 및/또는 야생형 리파제 폴리펩티드 또는 또 다른 조작된 리파제 폴리펩티드와 비교하여 임의의 효소 특성의 개선을 포함하는 조작된 리파제 폴리펩티드의 특성을 지칭한다. 개선된 특성은 증가된 단백질 발현, 증가된 열활성, 증가된 열안정성, 증가된 pH 활성, 증가된 안정성, 증가된 효소 활성, 증가된 기질 특이성 또는 친화성, 증가된 비활성, 기질 또는 최종 생성물 억제에 대한 증가된 내성, 증가된 화학적 안정성, 개선된 화학선택성, 개선된 용매 안정성, 산성 또는 염기성 pH에 대한 증가된 내성, 단백질분해 활성에 대한 증가된 내성(즉, 단백질분해에 대한 감소된 민감성), 감소된 응집, 증가된 용해도, 감소된 면역원성, 개선된 번역 후 변형(예컨대, 글리코실화) 및 변경된 온도 프로파일을 포함하지만 이에 제한되지 않는다.
본원에서 사용되는 바와 같이, "증가된 효소 활성" 또는 "향상된 촉매 활성"은 참조 리파제 효소와 비교하여 비활성(예컨대, 생성된 생성물/시간/중량 단백질)의 증가 또는 기질의 생성물로의 전환 백분율(예컨대, 특정 양의 리파제를 사용하여 특정 기간 동안 개시량의 기질의 생성물로의 전환 백분율)의 증가에 의해 나타낼 수 있는 조작된 리파제 폴리펩티드의 개선된 특성을 지칭한다. 효소 활성을 결정하기 위한 예시적인 방법이 실시예에 제공된다. K m , V max 또는 k cat 의 고전적인 효소 특성을 포함하여 효소 활성과 관련된 모든 특성이 영향을 받을 수 있으며, 이러한 특성의 변화는 효소 활성을 증가시킬 수 있다. 효소 활성은 상응하는 야생형 효소의 효소 활성의 약 1.1배에서 자연 발생 리파제 또는 리파제 폴리펩티드가 유도되는 또 다른 조작된 리파제보다 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배, 150배, 200배 또는 그 초과의 효소 활성까지 개선될 수 있다.
리파제 활성은 당업계에 공지된 임의의 적합한 방법(예컨대, 반응물 또는 생성물의 분광광도 특성의 변화를 모니터링하는 것과 같은 표준 검정)에 의해 측정될 수 있다. 일부 실시양태에서, 생성된 생성물의 양은 UV 흡광도와 조합된 고성능 액체 크로마토그래피(HPLC) 분리에 의해 측정될 수 있다. 일부 실시양태에서, 생성된 생성물의 양은 RAPIDFIRE® 질량 분광계를 사용하여 측정될 수 있는 반면, 일부 다른 실시양태에서 생성물은 당업계에 공지된 대안적인 방법을 이용하여 측정될 수 있다. 효소 활성의 비교는 효소의 정의된 제제, 설정된 조건 하의 정의된 분석, 및 하나 이상의 정의된 기질을 사용하여 이루어지며, 본원에서 더 자세히 설명된다.
본원에서 사용되는 바와 같이, 용어 "프로테아제에 안정한" 및 "단백분해에 대한 안정성"은 단백질(예컨대, 본 발명의 재조합 리파제)이 기능을 하고 임의의 단백질분해 효소 또는 다른 단백질분해 화합물 또는 인자에 의해 매개되는 단백질분해를 견디고 프로테아제에 노출된 후 이의 기능을 유지하는 능력을 지칭한다. 이 용어는 단백질의 안정성을 평가하기 위해 임의의 특정 프로테아제의 사용으로 제한되는 것으로 의도되지 않는다. 실제로, 본 발명의 조작된 리파제는 안정하고 다양한 프로테아제의 존재 또는 노출 후에 효소 활성을 유지한다. 일부 실시양태에서, 조작된 리파제는 트립신, 키모트립신 및/또는 펩신의 존재 하에 안정하다. 그러나, 본 발명은 임의의 특정 프로테아제 또는 단백질분해 안정성을 평가하는 임의의 특정 방법으로 제한되는 것으로 의도되지 않는다.
본원에서 사용되는 바와 같이, 용어 "pH 안정성"은 특정 pH에서 인큐베이션 후에 기능하는 단백질(예컨대, 본 발명의 재조합 리파제)의 능력을 지칭한다. 일부 실시양태에서, 본 발명은 pH 2 내지 pH 7의 범위를 포함하지만 이에 제한되지 않는 pH 범위에서 안정한 재조합 리파제를 제공한다. 일부 실시양태에서, 재조합 리파제는 본원에 제공된 실시예에 의해 지시되는 바와 같이 상이한 pH 범위에서 안정하다. 본 발명은 임의의 pH 안정성 수준 또는 pH 범위로 제한되는 것으로 의도되지 않는다.
본원에서 사용되는 바와 같이, 용어 "산성 pH에 대한 개선된 내성"은 본 발명에 따른 재조합 리파제가 참조 리파제 또는 또 다른 효소와 비교하여 증가된 안정성(특정 기간[예컨대, 1시간, 최대 24시간 등] 동안 산성 pH에 노출된 후 약 pH 7, 6, 5, 4, 3, 2, 또는 그 미만의 pH에서 더 높은 보유된 활성)을 가질 것이라는 것을 의미한다.
본원에서 사용되는 바와 같이, "생리학적 pH"는 대상체(예컨대, 인간)의 혈액(예컨대, pH 7.2 - 7.4)에서 일반적으로 발견되는 pH 범위를 의미한다.
(예컨대, 염기성 pH 조건에 대한 개선된 안정성 또는 염기성 pH에 대한 증가된 내성과 관련하여 사용되는) 용어 "염기성 pH"는 약 7 내지 11, 또는 일부 실시양태에서는 pH 11 초과의 pH 범위를 의미한다.
(예컨대, 산성 pH 조건에 대한 개선된 안정성 또는 산성 pH에 대한 증가된 내성과 관련하여 사용되는) 용어 "산성 pH"는 7 미만의 임의의 pH 값을 포괄하는 pH 범위를 의미한다. 일부 실시양태에서 산 pH는 7 미만인 반면, 일부 다른 실시양태에서 pH는 약 6, 5, 4, 3, 2 미만 또는 그 미만이다. 일부 대안적인 실시양태에서, 본 발명의 재조합 리파제는 2 내지 4의 pH 수준에서 안정하다. 그러나, 본 발명은 임의의 특정 pH 값 또는 값의 범위로 제한되는 것으로 의도되지 않는다.
본원에서 사용되는 바와 같이, 어구 "위 도전"은 재조합 리파제가 위(예컨대, 인간의 위)에서 마주칠 수 있는 조건에 노출되도록 본 발명의 재조합 리파제가 낮은 pH 환경 및 적어도 하나의 효소(예컨대, 펩신)의 존재에 노출되는 것을 지칭한다.
본원에서 사용되는 바와 같이, 어구 "장 도전"은 재조합 리파제가 장관(예컨대, 인간의 장)에서 마주칠 수 있는 조건에 노출되도록 본 발명의 재조합 리파제가 중성 pH 환경 및 트립신 및 키모트립신과 같은 장 프로테아제, 및 적어도 하나의 담즙염(예컨대, 나트륨 타우로콜레이트)의 존재에 노출되는 것을 지칭한다.
본원에서 사용되는 바와 같이, 어구 "서열에서 다중 도전"은 본 발명의 재조합 리파제가 일련의 도전 조건에 노출되는 것을 지칭한다. 예컨대, 일부 실시양태에서, 1시간의 가열 도전에 이어서 1시간의 위 도전이 뒤따르고 이어서 1시간의 장 도전이 뒤따른다. 본 발명은 임의의 특정 도전 및/또는 도전 조건 또는 특정 도전 서열로 제한되는 것으로 의도되지 않는다.
용어 "열 안정성" 및 "열안정성"은 특정 온도에서 기능하는 단백질(예컨대, 본 발명의 재조합 리파제)의 능력을 지칭한다. 일부 실시양태에서, 이 용어는 특정 온도에서 인큐베이션 후에 기능하는 단백질의 능력을 지칭한다. 일부 실시양태에서, 본 발명의 재조합 리파제는 "내열성"이다(즉, 효소는 상승된 온도에서 촉매 활성을 유지함). 일부 실시양태에서, 재조합 리파제는 승온에서 불활성화에 저항하고, 일부 추가 실시양태에서 연장된 노출 시간 동안 승온에서 촉매 활성을 유지한다. 이러한 용어는 본원에서 상호교환적으로 사용된다. 본 발명은 임의의 특정 온도 및/또는 노출 시간으로 제한되는 것으로 의도되지 않는다. 이러한 안정성은 당업계에 공지된 임의의 방법(예컨대, 본원에 기재된 방법)에 의해 측정될 수 있다. 본 발명은 임의의 특정 온도 안정성 수준 또는 온도 범위로 제한되는 것으로 의도되지 않는다. 일부 실시양태에서, 열 안정성은 특정 온도에서 단백질(예컨대, 본 발명의 재조합 리파제)의 인큐베이션 후에 측정된다.
용어 "화학적 안정성"은 또 다른 단백질의 기능에 악영향을 미치는 화학물질의 존재 하에 기능하는 단백질(예컨대, 본 발명의 재조합 리파제)의 능력을 지칭한다. 본 발명은 임의의 특정 화학적 안정성 수준 또는 화학적 안정성의 범위로 제한되는 것으로 의도되지 않는다.
"전환"은 기질의 상응하는 생성물(들)로의 효소적 전환 (또는 생체변환)을 지칭한다. "전환 백분율"은 특정 조건 하에서 소정의 기간 내에 생성물로 전환되는 기질의 백분율을 나타낸다. 따라서, 리파제 폴리펩티드의 "효소 활성" 또는 "활성"은 특정 기간에 기질의 생성물로의 "전환 백분율"로 표현될 수 있다.
본원에서 사용되는 바와 같이, "혼성화 엄격성"은 핵산의 혼성화에서 세척 조건과 같은 혼성화 조건에 관한 것이다. 일반적으로, 혼성화 반응은 더 낮은 엄격 조건 하에서 수행된 후 다양하지만 더 높은 엄격 세척이 수행된다. 용어 "적당히 엄격한 혼성화"는 표적 DNA가 표적 DNA에 대해 약 60% 동일성, 바람직하게는 약 75% 동일성, 약 85% 동일성, 표적 폴리뉴클레오티드에 대해 약 90% 초과의 동일성을 갖는 상보적 핵산에 결합하는 것을 허용하는 조건을 지칭한다. 예시적인 적당히 엄격한 조건은 42℃에서 50% 포름아미드, 5×덴하르트(Denhart) 용액, 5×SSPE, 0.2% SDS에서의 혼성화에 이어서 42℃에서 0.2×SSPE, 0.2% SDS에서의 세척과 동등한 조건이다. "높은 엄격한 혼성화"는 일반적으로 정의된 폴리뉴클레오티드 서열에 대한 용액 조건 하에 결정된 열 용융 온도 T m 으로부터 약 10℃ 이하인 조건을 지칭한다. 일부 실시양태에서, 높은 엄격한 조건은 65℃에서 0.018 M NaCl에서 안정한 하이브리드를 형성하는 핵산 서열만의 하이브리드화를 허용하는 조건을 지칭한다(즉, 하이브리드가 65℃에서 0.018 M NaCl에서 안정하지 않은 경우, 본원에서 고려되는 바와 같이 높은 엄격한 조건 하에서 안정하지 않을 것임). 예컨대, 42℃에서 50% 포름아미드, 5X 덴하르트 용액, 5×SSPE, 0.2% SDS와 동등한 조건에서 혼성화한 후 65℃에서 0.1×SSPE 및 0.1% SDS에서 세척함으로써 높은 엄격한 조건을 제공할 수 있다. 또 다른 높은 엄격한 조건은 65℃에서 0.1%(w/v) SDS를 함유하는 5×SSC에서 혼성화하고 65℃에서 0.1% SDS를 함유하는 0.1×SSC에서 세척하는 것과 동등한 조건에서 혼성화하는 것이다. 다른 높은 엄격한 혼성화 조건 뿐만 아니라 적당히 엄격한 조건은 상기에 인용된 참조 문헌에 설명되어 있다.
본원에서 사용되는 바와 같이, "벡터"는 DNA 서열을 세포로 도입하기 위한 DNA 구축물이다. 일부 실시양태에서, 벡터는 적합한 숙주에서 DNA 서열에 코딩된 폴리펩티드의 발현을 수행할 수 있는 적합한 제어 서열에 작동 가능하게 연결된 발현 벡터이다. 일부 실시양태에서, 플라스미드가 벡터로서 사용된다. 일부 실시양태에서, "발현 벡터"는 숙주 세포에서 발현을 유도하기 위해 DNA 서열(예컨대, 트랜스진)에 작동 가능하게 연결된 프로모터 서열을 갖고, 일부 실시양태에서 전사 터미네이터 서열도 포함한다.
본원에서 사용되는 바와 같이, "코돈 최적화된"은 코딩된 단백질이 관심 유기체에서 보다 효율적으로 발현되도록 단백질을 코딩하는 폴리뉴클레오티드의 코돈의 특정 유기체에서 우선적으로 사용되는 것으로의 변화를 지칭한다. 유전 암호는 대부분의 아미노산이 "시노님" 또는 "동의적" 코돈으로 불리는 여러 코돈으로 표시된다는 점에서 축퇴되지만, 특정 유기체에 의한 코돈 사용은 무작위가 아니며 특정 코돈 삼중자로 편향된다는 것은 널리 공지되어 있다. 이 코돈 사용 편향은 주어진 유전자, 공통 기능 또는 조상 기원의 유전자, 고도로 발현된 단백질 대 낮은 카피 수의 단백질, 및 유기체 게놈의 응집 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, 리파제 효소를 코딩하는 폴리뉴클레오티드는 발현을 위해 선택된 숙주 유기체로부터 최적의 생성을 위해 최적화된 코돈일 수 있다.
본원에서 사용되는 바와 같이, "제어 서열"은 본 출원의 폴리뉴클레오티드 및/또는 폴리펩티드의 발현에 필요하거나 유리한 모든 성분을 지칭한다. 각각의 제어 서열은 폴리펩티드를 코딩하는 핵산 서열에 대해 고유하거나 외래일 수 있다. 이러한 제어 서열은 리더, 폴리아데닐화 서열, 프로펩티드 서열, 프로모터 서열, 신호 펩티드 서열, 개시 서열 및 전사 터미네이터를 포함하지만 이에 제한되지 않는다. 최소한으로, 제어 서열은 프로모터, 전사 및 번역 정지 신호를 포함한다. 제어 서열은 폴리펩티드를 코딩하는 핵산 서열의 코딩 영역과 제어 서열의 리게이션을 용이하게 하는 특정 제한 부위를 도입할 목적으로 링커와 함께 제공될 수 있다.
"작동 가능하게 연결된"은 본원에서 제어 서열이 관심 폴리뉴클레오티드 및/또는 폴리펩티드 발현을 지시하거나 조절하도록 제어 서열이 관심 폴리뉴클레오티드와 관련된 위치에 적절하게 배치되는 구성(즉, 기능적 관계)으로 정의된다.
본원에서 사용되는 바와 같이, "프로모터 서열"은 코딩 서열과 같은 관심 폴리뉴클레오티드의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 프로모터 서열은 관심 폴리뉴클레오티드의 발현을 매개하는 전사 제어 서열을 함유한다. 프로모터는 돌연변이, 말단절단 및 하이브리드 프로모터를 포함하는 선택된 숙주 세포에서 전사 활성을 나타내는 임의의 핵산 서열일 수 있고, 숙주 세포에 대해 동종 또는 이종인 세포외 또는 세포내 폴리펩티드를 코딩하는 유전자로부터 수득될 수 있다.
본원에서 사용되는 바와 같이, 어구 "적합한 반응 조건"은 본 출원의 리파제 폴리펩티드가 기질을 원하는 생성물 화합물로 전환시킬 수 있는 효소 전환 반응 용액에서의 조건(예컨대, 효소 로딩, 기질 로딩, 온도, pH, 버퍼, 공용매 등의 범위)을 지칭한다. 예시적인 "적합한 반응 조건"은 본 출원에 제공되고 실시예에 의해 예시된다.
본원에서 사용되는 바와 같이, "화합물 로딩" 또는 "효소 로딩"과 같은 "로딩"은 반응 시작 시 반응 혼합물 중 성분의 농도 또는 양을 지칭한다.
본원에서 사용되는 바와 같이, 효소적 전환 반응과 관련하여 사용되는 용어 "기질"은 리파제 폴리펩티드에 의해 작용되는 화합물 또는 분자를 지칭한다.
본원에서 사용되는 바와 같이, 효소적 전환 과정과 관련하여 사용되는 용어 "생성물"은 기질에 대한 리파제 폴리펩티드의 작용으로부터 생성되는 화합물 또는 분자를 지칭한다.
본원에서 사용되는 바와 같이, 용어 "발현"은 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이에 제한되지 않는 폴리펩티드의 생성에 관련된 임의의 단계를 포함한다. 일부 실시양태에서, 이 용어는 또한 세포로부터 폴리펩티드의 분비를 포괄한다.
본원에서 사용되는 바와 같이, 용어 "생성하다"는 세포에 의한 단백질 및/또는 다른 화합물의 생성을 지칭한다. 이 용어는 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이에 제한되지 않는 폴리펩티드의 생성에 관련된 임의의 단계를 포괄하는 것으로 의도된다. 일부 실시양태에서, 이 용어는 또한 세포로부터 폴리펩티드의 분비를 포괄한다.
본원에서 사용되는 바와 같이, 아미노산 또는 뉴클레오티드 서열(예컨대, 프로모터 서열, 신호 펩티드, 터미네이터 서열 등)은 2개의 서열이 자연에서 연관되지 않는 경우 작동 가능하게 연결된 또 다른 서열에 대해 "이종"이다.
본원에서 사용되는 바와 같이, 용어 "숙주 세포" 및 "숙주 균주"는 본원에 제공된 DNA(예컨대, 리파제 변이체를 코딩하는 폴리뉴클레오티드)를 포함하는 발현 벡터에 적합한 숙주를 지칭한다. 일부 실시양태에서, 숙주 세포는 당업계에 공지된 재조합 DNA 기술을 이용하여 구축된 벡터로 형질전환되거나 형질감염된 원핵성 또는 진핵성 세포이다.
본원에서 사용되는 바와 같이, 용어 "유사체"는 참조 폴리펩티드와 70% 초과의 서열 동일성, 그러나 100% 미만의 서열 동일성(예컨대, 75%, 78%, 80%, 83%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 초과의 서열 동일성)을 갖는 폴리펩티드를 지칭한다. 일부 실시양태에서, 유사체는 호모아르기닌, 오르니틴 및 노르발린 뿐만 아니라 자연 발생 아미노산을 포함하지만 이에 제한되지 않는 하나 이상의 비-자연 발생 아미노산 잔기를 함유하는 폴리펩티드를 포함한다. 일부 실시양태에서, 유사체는 또한 1개 이상의 D-아미노산 잔기 및 2개 이상의 아미노산 잔기 사이의 비-펩티드 연결을 포함한다.
본원에서 사용되는 바와 같이, 용어 "배양"은 임의의 적합한 조건(예컨대, 액체, 겔 또는 고체 배지 사용) 하에서 세포 집단의 성장을 지칭한다. 일부 실시양태에서 세포는 미생물 세포(예컨대, 박테리아)인 반면, 일부 다른 실시양태에서 세포는 포유동물 세포, 곤충 세포, 또는 다른 동물로부터 수득되는 세포이다. 본 발명은 임의의 특정 세포 또는 세포 유형의 배양 또는 임의의 특정 배양 방법으로 제한되는 것으로 의도되지 않는다. 실제로, 본 발명은 임의의 적합한 조건 하에서 배양된 임의의 적합한 세포 유형을 포괄하는 것으로 의도된다.
용어 "치료제"는 병리의 징후 또는 증상을 나타내는 대상체에게 투여되는, 의학적 효과를 포함하여 유익하거나 바람직한 효과를 제공하는 화합물을 지칭한다.
용어 "약학적 조성물"은 약학적 유효량의 본 발명에 의해 포괄되는 조작된 리파제 폴리펩티드 및 허용되는 담체를 포함하는 대상체(예컨대, 인간)에서의 약학적 사용에 적합한 조성물을 지칭한다.
용어 "유효량"은 원하는 결과를 생성하기에 충분한 양을 의미한다. 당업자는 통상적인 실험을 사용하여 유효량을 결정할 수 있다.
용어 "대상체"는 인간, 비-인간 영장류, 가축, 반려 동물, 및 실험 동물(예컨대, 설치류 및 래거모프)과 같은 포유동물을 포함하지만 이에 제한되지 않는 동물을 포함한다. 이 용어는 수컷 뿐만 아니라 암컷도 포함하는 것으로 의도된다.
본원에서 사용되는 바와 같이, 용어 "환자"는 질환에 대해 평가되고 있거나, 치료를 받거나, 겪고 있는 임의의 대상체를 의미한다.
용어 "유아"는 생후 1개월부터 대략 1세까지의 기간에 있는 소아를 지칭한다.
본원에서 사용되는 바와 같이, 용어 "신생아"는 생후부터 28일까지의 기간의 소아를 의미한다. 용어 "미숙아"는 임신 20주 이후에 태어나지만 만삭이 되기 전에 태어난 유아를 지칭하며 일반적으로 출생 시 체중이 ~500 내지 ~2499 g이다. "초저체중 출생아"는 출생 시 체중이 1500 g 미만인 유아이다.
본원에서 사용되는 바와 같이, 용어 "소아"는 치료 또는 연구 절차에 동의할 법적 연령에 도달하지 않은 사람을 지칭한다. 일부 실시양태에서, 이 용어는 출생시와 청소년기 사이의 사람을 지칭한다.
본원에서 사용되는 바와 같이, 용어 "성인"은 관련 권한에 대한 법적 연령(예컨대, 미국에서 18세)에 도달한 사람을 지칭한다. 일부 실시양태에서, 이 용어는 임의의 완전히 성장한 성숙한 유기체를 지칭한다. 일부 실시양태에서, 용어 "젊은 성인"은 18세 미만이지만 성적 성숙에 도달한 사람을 지칭한다.
본원에서 사용되는 바와 같이, "조성물" 및 "제형"은 임의의 적합한 용도(예컨대, 약학적 조성물, 식이 및/또는 영양 보충제, 사료 등)를 위한 본 발명의 적어도 하나의 조작된 리파제를 포함하는 생성물을 포괄한다.
본원에서 사용되는 바와 같이, 용어 "투여" 및 "투여하는"은 본 발명의 조성물을 대상체(예컨대, 췌장 기능부전의 영향으로 고통받는 사람)에게 제공하는 것을 의미한다.
약학적 조성물과 관련하여 본원에서 사용될 때 용어 "담체"는 임의의 표준 약학적 담체, 버퍼, 및 부형제, 예컨대 안정화제, 보존제 및 보조제를 의미한다.
용어 "약학적으로 허용되는"은 바람직하지 않은 생물학적 효과를 일으키지 않거나 물질이 이에 함유되고 원하는 생물학적 활성을 보유하는 임의의 성분과 유해한 방식으로 상호작용하지 않으면서 대상체에게 투여될 수 있는 물질을 의미한다.
본원에서 사용되는 바와 같이, 용어 "부형제"는 활성 약학적 성분(API; 예컨대, 본 발명의 조작된 리파제 폴리펩티드) 이외의 임의의 약학적으로 허용되는 첨가제, 담체, 희석제, 애주번트 또는 다른 성분을 지칭한다. 부형제는 전형적으로 제형 및/또는 투여 목적으로 포함된다.
용어 "치료학적 유효량"은 질환/병태의 증상과 관련하여 사용될 때 질환/병태의 하나 이상의 증상을 개선, 약화 또는 제거하거나 증상(들)의 발병을 예방 또는 지연시키는 화합물(예컨대, 조작된 리파제 폴리펩티드)의 양 및/또는 농도를 지칭한다.
용어 "치료학적 유효량"은 질환/병태와 관련하여 사용될 때 질환/병태를 개선, 약화 또는 제거하는 조성물(예컨대, 조작된 리파제 폴리펩티드)의 양 및/또는 농도를 지칭한다. 일부 실시양태에서, 이 용어는 연구원, 의사, 수의사 또는 다른 임상의가 찾는 조직, 시스템 또는 동물 대상체에 의한 생물학적(예컨대, 의학적) 반응을 유도하는 조성물의 양과 관련하여 사용된다.
본원에서 사용되는 바와 같이, 용어 "치료하는", "치료하다", 및 "치료"는 본원에 제공된 것과 같은 약학적 조성물의 투여를 포함하여 대상체(예컨대, 인간 환자)에게 제공되는 의료를 지칭한다. 용어 "치료하는", "치료하다" 및 "치료"는 예방적(preventative)(예컨대, 예방적(prophylactic)) 뿐만 아니라 고식적 치료 또는 치유를 포함하는 것으로 의도된다. 일부 실시양태에서, 질환의 증상을 예방하거나 개선하기 위한 치료가 제공된다. 일부 실시양태에서, 본 발명의 약학적 조성물은 췌장 효소 기능부전 질환 또는 병태의 치료 또는 예방에 사용된다.
조작된 리파제:
본 발명은 췌장 효소 기능부전의 치료를 포함한 다양한 용도에 적합한 조작된 리파제를 제공한다. 일부 실시양태에서, 개선된 특성을 나타내는 조작된 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 적어도 약 85%, 적어도 약 88%, 적어도 약 90%, 적어도 약 91%, 적어도 약 92%, 적어도 약 93%, 적어도 약 94%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 적어도 약 99%, 또는 약 100% 아미노산 서열 동일성, 및 하나 이상의 아미노산 위치(예컨대, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30개, 또는 그 초과의 아미노산 위치)에서 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 비교하여 아미노산 잔기 차이, 또는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 적어도 85%, 적어도 88%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 적어도 99% 또는 그 초과의 아미노산 서열 동일성을 갖는 서열을 갖는다. 일부 실시양태에서, 하나 이상의 위치에서 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 비교하여 잔기 차이는 적어도 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 11, 12, 14, 15, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30개, 또는 그 초과의 보존적 아미노산 치환을 포함한다. 그러나, 본 발명은 다른 치환이 본 발명에서 사용되므로 보존적 아미노산 치환을 갖는 리파제 변이체로 제한되는 것으로 의도되지 않는다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 열거된 폴리펩티드이다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868을 포함한다.
본 발명은 또한 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 적어도 하나의 재조합 리파제를 제공하며, 재조합 리파제는 2, 3, 4, 22, 27/46/97/136/149/385, 27/46/385, 27/70/136/231/385, 27/136/323/385, 27/149, 27/149/385, 27/385, 34, 38, 41, 44, 46/70, 46/149/183/231/385, 48, 70, 73, 73/305, 82, 82/94/101/194/199, 82/94/199, 83, 85, 87, 89, 92, 94, 96, 97/149/385, 135, 136/385, 140, 141, 142, 144, 146, 149, 149/231/385, 151, 174, 175, 178, 181, 183/385, 189, 194, 194/199, 195, 195/231/385, 199, 199/213, 210, 212, 213, 213/330, 216, 218, 219, 226, 231/385, 238, 247, 250, 270, 274, 281, 292, 296, 300, 308, 330, 338, 379, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 2H, 2M, 2T, 3A, 3G, 3R, 3S, 4M, 4W, 4Y, 22N, 27V/46T/97A/136V/149E/385P, 27V/46T/385P, 27V/70N/136V/231E/385P, 27V/136V/323I/385P, 27V/149E, 27V/149E/385P, 27V/385P, 34D, 38A, 38H, 38L, 41V, 44E, 46T/70N, 46T/149E/183L/231E/385P, 48V, 70N, 73C/305I, 73I, 73R, 82C, 82E, 82E/94S/101S/194N/199L, 82E/94S/199L, 82F, 82G, 82L, 83G, 83K, 85I, 85W, 87R, 89A, 89T, 89V, 89W, 92A, 94S, 96E, 96N, 96S, 97A/149E/385P, 135G, 135Q, 135S, 135T, 135V, 136V/385P, 140T, 141A, 141F, 141L, 141S, 142A, 142F, 142I, 142L, 144M, 144R, 144W, 146A, 146F, 146S, 146Y, 149E, 149E/231E/385P, 149K, 149R, 151A, 151I, 151L, 174H, 174R, 175G, 175L, 175R, 178R, 178T, 181H, 183L/385P, 189A, 189E, 189W, 194N, 194N/199L, 194T, 195D/231E/385P, 195V, 199L, 199L/213A, 210A, 210V, 212C, 212G, 212R, 212T, 213A/330T, 213H, 216R, 216T, 216W, 218D, 218G, 218I, 218M, 218T, 219C, 219G, 219K, 219R, 226R, 231E/385P, 238S, 247R, 250T, 270V, 274D, 281K, 281R, 292A, 292C, 292L, 292V, 296M, 296R, 300A, 300D, 300T, 308A, 330Y, 338N, 379T, 385A, 385C, 385D, 385P, 385R, 및 385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 E2H, E2M, E2T, T3A, T3G, T3R, T3S, S4M, S4W, S4Y, G22N, Y27V/R46T/Y97A/Y136V/T149E/F385P, Y27V/R46T/F385P, Y27V/K70N/Y136V/Q231E/F385P, Y27V/Y136V/K323I/F385P, Y27V/T149E, Y27V/T149E/F385P, Y27V/F385P, K34D, F38A, F38H, F38L, N41V, G44E, R46T/K70N, R46T/T149E/F183L/Q231E/F385P, Y48V, K70N, T73C/V305I, T73I, T73R, K82C, K82E, K82E/P94S/D101S/K194N/I199L, K82E/P94S/I199L, K82F, K82G, K82L, E83G, E83K, G85I, G85W, A87R, F89A, F89T, F89V, F89W, T92A, P94S, I96E, I96N, I96S, Y97A/T149E/F385P, Y135G, Y135Q, Y135S, Y135T, Y135V, Y136V/F385P, P140T, E141A, E141F, E141L, E141S, E142A, E142F, E142I, E142L, I144M, I144R, I144W, P146A, P146F, P146S, P146Y, T149E, T149E/Q231E/F385P, T149K, T149R, G151A, G151I, G151L, E174H, E174R, Q175G, Q175L, Q175R, S178R, S178T, K181H, F183L/F385P, S189A, S189E, S189W, K194N, K194N/I199L, K194T, Q195D/Q231E/F385P, Q195V, I199L, I199L/P213A, K210A, K210V, Q212C, Q212G, Q212R, Q212T, P213A/N330T, P213H, S216R, S216T, S216W, H218D, H218G, H218I, H218M, H218T, A219C, A219G, A219K, A219R, T226R, Q231E/F385P, Y238S, E247R, Q250T, R270V, T274D, G281K, G281R, M292A, M292C, M292L, M292V, S296M, S296R, Q300A, Q300D, Q300T, R308A, N330Y, K338N, N379T, F385A, F385C, F385D, F385P, F385R, 및 F385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제 A는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 3/4/96/296/300, 3/292/296, 4, 4/11/149/292/296/300, 4/96/149/231/296/300, 4/96/149/292/296/300, 4/96/219/296, 4/96/231/296/300, 4/96/292/296/300, 4/149/174, 4/174/219/292/296, 4/231/296/300, 27, 27/34/82, 27/73/82/218, 27/89, 27/89/178, 27/89/218, 27/218, 34/73/218, 34/82, 34/218, 73/82, 73/82/183, 94/146/175, 96/149/174/292/296, 96/149/231/292/296, 96/149/292/296, 96/174/219/231/292/296, 96/231/296, 146/175/189/281, 149/174/292/296, 149/174/300, 149/219/231/292/296/300, 149/231/292/296, 149/231/292/296/300, 149/296, 149/296/300, 174/231, 174/296, 218, 231, 231/292/296, 231/292/296/300, 231/296, 231/300, 292/296, 292/296/300, 296, 및 296/300으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 3S/4W/96E/296R/300D, 3S/292L/296R, 4W, 4W/11A/149E/292L/296R/300D, 4W/96E/149E/231E/296R/300D, 4W/96E/149E/292L/296R/300D, 4W/96E/219K/296R, 4W/96E/231E/296R/300D, 4W/96E/292L/296R/300D, 4W/149E/174R, 4W/174H/219K/292L/296R, 4W/231E/296R/300D, 27V, 27V/34D/82E, 27V/73I/82L/218I, 27V/73I/82L/218M, 27V/89T, 27V/89T/178P, 27V/89T/218I, 27V/218I, 34D/73I/218I, 34D/82C, 34D/218M, 73I/82C, 73I/82L/183L, 94S/146F/175G, 96E/149E/174H/292L/296R, 96E/149E/231E/292L/296R, 96E/149E/292L/296R, 96E/174H/219K/231E/292L/296R, 96E/231E/296R, 146F/175G/189A/281K, 149E/174H/292L/296R, 149E/174H/300D, 149E/219K/231E/292L/296R/300D, 149E/231E/292L/296R, 149E/231E/292L/296R/300D, 149E/296R, 149E/296R/300D, 174H/296R, 174R/231E, 218I, 231E, 231E/292L/296R, 231E/292L/296R/300D, 231E/296R, 231E/300D, 292L/296R, 292L/296R/300D, 296R, 및 296R/300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 T3S/S4W/I96E/S296R/Q300D, T3S/M292L/S296R, S4W, S4W/V11A/T149E/M292L/S296R/Q300D, S4W/I96E/T149E/Q231E/S296R/Q300D, S4W/I96E/T149E/M292L/S296R/Q300D, S4W/I96E/A219K/S296R, S4W/I96E/Q231E/S296R/Q300D, S4W/I96E/M292L/S296R/Q300D, S4W/T149E/E174R, S4W/E174H/A219K/M292L/S296R, S4W/Q231E/S296R/Q300D, Y27V, Y27V/K34D/K82E, Y27V/T73I/K82L/H218I, Y27V/T73I/K82L/H218M, Y27V/F89T, Y27V/F89T/S178P, Y27V/F89T/H218I, Y27V/H218I, K34D/T73I/H218I, K34D/K82C, K34D/H218M, T73I/K82C, T73I/K82L/F183L, P94S/P146F/Q175G, I96E/T149E/E174H/M292L/S296R, I96E/T149E/Q231E/M292L/S296R, I96E/T149E/M292L/S296R, I96E/E174H/A219K/Q231E/M292L/S296R, I96E/Q231E/S296R, P146F/Q175G/S189A/G281K, T149E/E174H/M292L/S296R, T149E/E174H/Q300D, T149E/A219K/Q231E/M292L/S296R/Q300D, T149E/Q231E/M292L/S296R, T149E/Q231E/M292L/S296R/Q300D, T149E/S296R, T149E/S296R/Q300D, E174H/S296R, E174R/Q231E, H218I, Q231E, Q231E/M292L/S296R, Q231E/M292L/S296R/Q300D, Q231E/S296R, Q231E/Q300D, M292L/S296R, M292L/S296R/Q300D, S296R, 및 S296R/Q300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 4, 4/27/189/300, 4/70, 4/102, 4/137, 4/175/189/218, 4/175/189/300, 4/175/218/224, 4/175/218/373, 4/185, 4/189/218/373, 4/193, 4/218/300/369/373, 4/228, 4/233, 4/243, 4/271, 4/303, 4/334, 4/336, 4/375, 25, 27/82/174/175/189/218/300/369/373, 27/82/174/175/218/300/369, 27/82/174/175/218/300/373/382, 27/82/174/218/300/373, 27/189/218/373, 28, 33, 99, 102, 104, 134, 153, 174/175/189, 174/373, 175/189/218/300/373, 175/189/373, 175/218, 175/218/382, 189/218/300/373, 191, 193, 231, 233, 243, 293, 303, 331, 336, 339, 368, 및 373으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 4W, 4W/27V/189A/300D, 4W/70H, 4W/102T, 4W/137R, 4W/175G/189A/218M, 4W/175G/189A/300D, 4W/175G/218M/224A, 4W/175G/218M/373F, 4W/185L, 4W/189A/218M/373F, 4W/193T, 4W/218M/300D/369N/373F, 4W/228E, 4W/233R, 4W/243R, 4W/271D, 4W/303P, 4W/334T, 4W/336S, 4W/336T, 4W/375A, 25V, 27V/82C/174H/175G/189A/218M/300D/369N/373F, 27V/82C/174H/175G/218M/300D/369N, 27V/82C/174H/175G/218M/300D/373F/382M, 27V/82C/174H/218M/300D/373F, 27V/189A/218M/373F, 28N, 33Y, 99D, 102L, 104H, 134R, 153G, 174H/175L/189A, 174H/373F, 175G/189A/218M/300D/373F, 175G/218M, 175G/218M/382M, 175L/189A/373F, 189A/218M/300D/373F, 191L, 193L, 231Q, 233G, 233S, 233W, 243R, 293R, 303P, 331R, 336R, 339K, 368A, 368T, 및 373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 S4W, S4W/Y27V/S189A/Q300D, S4W/K70H, S4W/E102T, S4W/S137R, S4W/Q175G/S189A/H218M, S4W/Q175G/S189A/Q300D, S4W/Q175G/H218M/V224A, S4W/Q175G/H218M/Y373F, S4W/I185L, S4W/S189A/H218M/Y373F, S4W/Q193T, S4W/H218M/Q300D/S369N/Y373F, S4W/P228E, S4W/N233R, S4W/L243R, S4W/G271D, S4W/A303P, S4W/D334T, S4W/A336S, S4W/A336T, S4W/N375A, L25V, Y27V/K82C/E174H/Q175G/S189A/H218M/Q300D/S369N/Y373F, Y27V/K82C/E174H/Q175G/H218M/Q300D/S369N, Y27V/K82C/E174H/Q175G/H218M/Q300D/Y373F/H382M, Y27V/K82C/E174H/H218M/Q300D/Y373F, Y27V/S189A/H218M/Y373F, R28N, L33Y, Q99D, E102L, N104H, E134R, N153G, E174H/Q175L/S189A, E174H/Y373F, Q175G/S189A/H218M/Q300D/Y373F, Q175G/H218M, Q175G/H218M/H382M, Q175L/S189A/Y373F, S189A/H218M/Q300D/Y373F, A191L, Q193L, E231Q, N233G, N233S, N233W, L243R, Q293R, A303P, S331R, A336R, Q339K, F368A, F368T, 및 Y373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제 A는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 26, 33, 33/174/193/243, 33/174/334, 33/175/218/303, 33/175/218/334/339, 33/193, 34, 38, 46, 48, 70/271/293/334, 144, 174, 174/175/193, 174/175/218/339, 174/193/303/375, 174/193/375, 174/218/233/271/293/303, 174/218/271/303, 175/193/218/233/243/375, 175/193/218/243, 175/218/375, 181, 189, 193, 193/218/243, 193/271/303/334, 193/293/303, 194, 195, 199, 210, 218, 218/243, 218/243/303/334, 218/303, 225, 238, 274, 281, 및 330으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 G26A, G26R, G26S, L33Y, L33Y/E174H/Q193L/L243R, L33Y/E174H/D334T, L33Y/L175G/H218M/A303P, L33Y/L175G/H218M/D334T/Q339K, L33Y/Q193L, K34L, F38A, F38G, R46P, Y48L, K70H/G271D/Q293R/D334T, I144L, E174H, E174H/L175G/Q193L, E174H/L175G/H218M/Q339K, E174H/Q193L/A303P/N375A, E174H/Q193L/N375A, E174H/H218M/N233R/G271D/Q293R/A303P, E174H/H218M/G271D/A303P, E174R, L175G/Q193L/H218M/N233R/L243R/N375A, L175G/Q193L/H218M/L243R, L175G/H218M/N375A, K181Q, A189H, Q193L, Q193L/H218M/L243R, Q193L/G271D/A303P/D334T, Q193L/Q293R/A303P, K194T, Q195I, Q195L, Q195Y, I199H, K210V, H218C, H218D, H218M, H218M/L243R, H218M/L243R/A303P/D334T, H218M/A303P, H218P, M225L, Y238W, T274D, G281K, G281P, N330F, 및 N330H로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제 A는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 34/38/174/193/195/243/281/303/330, 34/38/174/225/303, 34/38/174/303/345, 34/174, 34/174/193/195/243/281, 34/174/193/195/303/330, 34/193/195/225/243, 34/193/243/303/330, 34/281/330, 38, 38/174/193/195/243/330, 38/174/193/225/274/283/303/330, 38/174/193/303, 38/174/195/281/330, 38/174/225/243/281/330, 38/174/281, 38/174/281/303, 38/174/281/303/345, 38/193/195/225/303/345, 38/195/243/303, 38/195/281/303/330, 38/195/281/330, 38/195/303, 49, 49/51/98/252/311, 49/51/123/252/344, 49/98/120/252/344, 49/120/252/344, 49/123/252/344, 49/123/264/344, 49/123/311, 49/252/344, 49/311/344, 49/344, 51, 51/252/344, 51/344, 98, 98/344, 123/252/344, 129, 160, 161, 174/193/195/225, 174/193/303/330, 174/195/225/281/303/330/345, 174/195/243/281/345, 174/195/281/303, 174/225/243/281/303/345, 174/281/330, 174/303, 195/225/303/330, 252, 268, 및 344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 34L/38A/174G/193L/195L/243R/281P/303P/330F, 34L/38A/174R/303P/345I, 34L/38G/174R/225L/303P, 34L/174G, 34L/174G/193L/195L/243R/281P, 34L/174H/193L/195I/303P/330F, 34L/193L/195I/225L/243R, 34L/193L/243R/303P/330F, 34L/281P/330H, 38A/174H/193L/225L/274D/283I/303P/330F, 38A/174R/281K/303P/345I, 38A/193L/195L/225L/303P/345I, 38A/195I/303P, 38A/195Y/281K/303P/330F, 38G, 38G/174G/193L/303P, 38G/174G/281K/303P, 38G/174H/195L/281P/330F, 38G/174H/225L/243R/281K/330H, 38G/174H/281K, 38G/174R/193L/195L/243R/330H, 38G/195I/281P/330F, 38G/195Y/243R/303P, 49S, 49T, 49T/51A/98P/252V/311W, 49T/51A/123Q/252V/344H, 49T/98P/120T/252V/344H, 49T/120T/252V/344H, 49T/123Q/252V/344H, 49T/123Q/264S/344H, 49T/123Q/311W, 49T/252V/344H, 49T/311W/344H, 49T/344H, 51A/252V/344H, 51A/344H, 51V, 98P/344H, 98R, 123Q/252V/344H, 129F, 160T, 161I, 174G/195I/225L/281K/303P/330F/345I, 174G/225L/243R/281P/303P/345I, 174H/195I/243R/281K/345I, 174H/281P/330H, 174R/193L/195I/225L, 174R/193L/303P/330H, 174R/195I/281K/303P, 174R/303P, 195Y/225L/303P/330H, 252V, 268T, 344I, 344V, 및 344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 K34L/F38A/E174G/Q193L/Q195L/L243R/G281P/A303P/N330F, K34L/F38A/E174R/A303P/F345I, K34L/F38G/E174R/M225L/A303P, K34L/E174G, K34L/E174G/Q193L/Q195L/L243R/G281P, K34L/E174H/Q193L/Q195I/A303P/N330F, K34L/Q193L/Q195I/M225L/L243R, K34L/Q193L/L243R/A303P/N330F, K34L/G281P/N330H, F38A/E174H/Q193L/M225L/T274D/M283I/A303P/N330F, F38A/E174R/G281K/A303P/F345I, F38A/Q193L/Q195L/M225L/A303P/F345I, F38A/Q195I/A303P, F38A/Q195Y/G281K/A303P/N330F, F38G, F38G/E174G/Q193L/A303P, F38G/E174G/G281K/A303P, F38G/E174H/Q195L/G281P/N330F, F38G/E174H/M225L/L243R/G281K/N330H, F38G/E174H/G281K, F38G/E174R/Q193L/Q195L/L243R/N330H, F38G/Q195I/G281P/N330F, F38G/Q195Y/L243R/A303P, V49S, V49T, V49T/T51A/G98P/M252V/L311W, V49T/T51A/E123Q/M252V/S344H, V49T/G98P/M120T/M252V/S344H, V49T/M120T/M252V/S344H, V49T/E123Q/M252V/S344H, V49T/E123Q/T264S/S344H, V49T/E123Q/L311W, V49T/M252V/S344H, V49T/L311W/S344H, V49T/S344H, T51A/M252V/S344H, T51A/S344H, T51V, G98P/S344H, G98R, E123Q/M252V/S344H, S129F, S160T, L161I, E174G/Q195I/M225L/G281K/A303P/N330F/F345I, E174G/M225L/L243R/G281P/A303P/F345I, E174H/Q195I/L243R/G281K/F345I, E174H/G281P/N330H, E174R/Q193L/Q195I/M225L, E174R/Q193L/A303P/N330H, E174R/Q195I/G281K/A303P, E174R/A303P, Q195Y/M225L/A303P/N330H, M252V, S268T, S344I, S344V, 및 S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 11/16/67/168/180/287, 11/16/237/241/287, 11/67/168/287, 16/67/154/168/237/241/287, 16/67/237/287/291, 16/168/177, 24, 24/278, 34/49/161/193/243/344, 34/49/161/193/344, 34/49/161/243/344, 34/49/161/252/268/344, 34/49/193/243/252/344, 34/49/193/344, 34/49/243/252, 34/49/252/268/344, 34/161/193/243/252/268, 34/161/193/243/268/344, 34/161/193/243/344, 34/161/193/252/268/344, 34/161/193/252/344, 34/161/193/268/344, 34/161/243/281/344, 34/161/344, 34/193/243/252/344, 34/193/252/268/344, 34/193/268/281, 34/252/268/281/344, 34/252/344, 49/161/193/243/252/344, 49/161/193/252/281/344, 49/161/243/252/344, 49/193/197/243/252/281/344, 49/193/243/252/344, 49/193/243/344, 49/243/344, 67/154/237/287, 67/168/237, 67/168/237/287, 67/177/237, 154/237/286/287, 154/286/287, 161/193/252/344, 161/193/344, 161/344, 168/237, 186/187/278, 193/243/281/344, 193/252/268/344, 193/252/281/344, 237, 237/287/291, 및 281/344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 11I/16F/67A/168T/180V/287L, 11I/16F/237Q/241S/287L, 11I/67A/168T/287L, 16F/67A/154Y/168T/237Q/241S/287L, 16F/67A/237Q/287L/291A, 16F/168T/177L, 24M, 24M/278L, 34K/49T/161I/193L/243R/344H, 34K/49T/161I/193L/243R/344V, 34K/49T/161I/193L/344W, 34K/49T/161I/243R/344H, 34K/49T/161I/252V/268T/344V, 34K/49T/193L/243R/252V/344W, 34K/49T/193L/344H, 34K/49T/193L/344W, 34K/49T/243R/252V, 34K/49T/252V/268T/344V, 34K/161I/193L/243R/252V/268T, 34K/161I/193L/243R/268T/344V, 34K/161I/193L/243R/344W, 34K/161I/193L/252V/268T/344H, 34K/161I/193L/252V/268T/344V, 34K/161I/193L/252V/344H, 34K/161I/193L/252V/344V, 34K/161I/193L/252V/344W, 34K/161I/193L/268T/344H, 34K/161I/243R/281P/344V, 34K/161I/344V, 34K/161I/344W, 34K/193L/243R/252V/344W, 34K/193L/252V/268T/344H, 34K/193L/268T/281P, 34K/252V/268T/281P/344V, 34K/252V/344V, 49T/161I/193L/243R/252V/344W, 49T/161I/193L/252V/281P/344H, 49T/161I/243R/252V/344V, 49T/193L/197G/243R/252V/281P/344H, 49T/193L/243R/252V/344V, 49T/193L/243R/344W, 49T/243R/344V, 67A/154Y/237Q/287L, 67A/168T/237Q, 67A/168T/237Q/287L, 67A/177L/237Q, 154Y/237Q/286V/287L, 154Y/286V/287L, 161I/193L/252V/344V, 161I/193L/344W, 161I/344W, 168T/237Q, 186T/187A/278L, 193L/243R/281P/344W, 193L/252V/268T/344V, 193L/252V/281P/344H, 237Q, 237Q/287L/291A, 및 281P/344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 V11I/L16F/Y67A/S168T/I180V/F287L, V11I/L16F/A237Q/T241S/F287L, V11I/Y67A/S168T/F287L, L16F/Y67A/W154Y/S168T/A237Q/T241S/F287L, L16F/Y67A/A237Q/F287L/S291A, L16F/S168T/V177L, F24M, F24M/Y278L, L34K/V49T/L161I/Q193L/L243R/S344H, L34K/V49T/L161I/Q193L/L243R/S344V, L34K/V49T/L161I/Q193L/S344W, L34K/V49T/L161I/L243R/S344H, L34K/V49T/L161I/M252V/S268T/S344V, L34K/V49T/Q193L/L243R/M252V/S344W, L34K/V49T/Q193L/S344H, L34K/V49T/Q193L/S344W, L34K/V49T/L243R/M252V, L34K/V49T/M252V/S268T/S344V, L34K/L161I/Q193L/L243R/M252V/S268T, L34K/L161I/Q193L/L243R/S268T/S344V, L34K/L161I/Q193L/L243R/S344W, L34K/L161I/Q193L/M252V/S268T/S344H, L34K/L161I/Q193L/M252V/S268T/S344V, L34K/L161I/Q193L/M252V/S344H, L34K/L161I/Q193L/M252V/S344V, L34K/L161I/Q193L/M252V/S344W, L34K/L161I/Q193L/S268T/S344H, L34K/L161I/L243R/G281P/S344V, L34K/L161I/S344V, L34K/L161I/S344W, L34K/Q193L/L243R/M252V/S344W, L34K/Q193L/M252V/S268T/S344H, L34K/Q193L/S268T/G281P, L34K/M252V/S268T/G281P/S344V, L34K/M252V/S344V, V49T/L161I/Q193L/L243R/M252V/S344W, V49T/L161I/Q193L/M252V/G281P/S344H, V49T/L161I/L243R/M252V/S344V, V49T/Q193L/S197G/L243R/M252V/G281P/S344H, V49T/Q193L/L243R/M252V/S344V, V49T/Q193L/L243R/S344W, V49T/L243R/S344V, Y67A/W154Y/A237Q/F287L, Y67A/S168T/A237Q, Y67A/S168T/A237Q/F287L, Y67A/V177L/A237Q, W154Y/A237Q/L286V/F287L, W154Y/L286V/F287L, L161I/Q193L/M252V/S344V, L161I/Q193L/S344W, L161I/S344W, S168T/A237Q, H186T/L187A/Y278L, Q193L/L243R/G281P/S344W, Q193L/M252V/S268T/S344V, Q193L/M252V/G281P/S344H, A237Q, A237Q/F287L/S291A, 및 G281P/S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제 A는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24/168/252/281, 24/237/287/344, 24/252, 24/252/287, 24/344, 213/252/278/344, 252/278/344, 252/287/344, 및 281로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24M/168T/252V/281P, 24M/237Q/287L/344S, 24M/252V, 24M/252V/287L, 24M/344W, 213S/252V/278L/344S, 252V/278L/344W, 252V/287L/344S, 및 281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 F24M/S168T/M252V/G281P, F24M/A237Q/F287L/H344S, F24M/M252V, F24M/M252V/F287L, F24M/H344W, P213S/M252V/Y278L/H344S, M252V/Y278L/H344W, M252V/F287L/H344S, 및 G281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다.
일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24, 24/38/49/70/149/161/174/175/189/193/225/231/243/252/271/287/292/293/296/303/334/344/373/385, 38, 49, 70, 149, 161, 174, 175, 189, 193, 225, 231, 243, 252, 271, 287, 292, 293, 296, 303, 334, 344, 373, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 24A, 24C, 24D, 24E, 24F, 24F/38F/49V/70K/149T/161L/174E/175Q/189S/193Q/225M/231Q/243L/252M/271G/287F/292M/293Q/296S/303A/334D/344S/373Y/385F, 24G, 24H, 24I, 24K, 24L, 24N, 24P, 24Q, 24R, 24S, 24T, 24V, 24W, 24Y, 38A, 38C, 38D, 38E, 38F, 38H, 38I, 38K, 38L, 38M, 38N, 38P, 38Q, 38R, 38S, 38T, 38V, 38W, 38Y, 49A, 49C, 49D, 49E, 49F, 49G, 49H, 49I, 49K, 49L, 49M, 49N, 49P, 49Q, 49R, 49S, 49V, 49W, 49Y, 70A, 70C, 70D, 70E, 70F, 70G, 70I, 70K, 70L, 70M, 70N, 70P, 70Q, 70R, 70S, 70T, 70V, 70W, 70Y, 149A, 149C, 149D, 149F, 149G, 149H, 149I, 149K, 149L, 149M, 149N, 149P, 149Q, 149R, 149S, 149T, 149V, 149W, 149Y, 161A, 161C, 161D, 161E, 161F, 161G, 161H, 161K, 161L, 161M, 161N, 161P, 161Q, 161R, 161S, 161T, 161V, 161W, 161Y, 174A, 174C, 174D, 174E, 174F, 174G, 174H, 174I, 174K, 174L, 174M, 174N, 174P, 174Q, 174S, 174T, 174V, 174W, 174Y, 175A, 175C, 175D, 175E, 175F, 175G, 175H, 175I, 175K, 175M, 175N, 175P, 175Q, 175R, 175S, 175T, 175V, 175W, 175Y, 189C, 189D, 189E, 189F, 189G, 189H, 189I, 189K, 189L, 189M, 189N, 189P, 189Q, 189R, 189S, 189T, 189V, 189W, 189Y, 193A, 193C, 193D, 193E, 193F, 193G, 193H, 193I, 193K, 193M, 193N, 193P, 193Q, 193R, 193S, 193T, 193V, 193W, 193Y, 225A, 225C, 225D, 225E, 225F, 225G, 225H, 225I, 225K, 225M, 225N, 225P, 225Q, 225R, 225S, 225T, 225V, 225W, 225Y, 231A, 231C, 231D, 231F, 231G, 231H, 231I, 231K, 231L, 231M, 231N, 231P, 231Q, 231R, 231S, 231T, 231V, 231W, 231Y, 243A, 243C, 243D, 243E, 243F, 243G, 243H, 243I, 243K, 243L, 243M, 243N, 243P, 243Q, 243S, 243T, 243V, 243W, 243Y, 252A, 252C, 252D, 252E, 252F, 252G, 252H, 252I, 252K, 252L, 252M, 252N, 252P, 252Q, 252R, 252S, 252T, 252W, 252Y, 271A, 271C, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 287A, 287C, 287D, 287E, 287F, 287G, 287H, 287I, 287K, 287M, 287N, 287P, 287Q, 287R, 287S, 287T, 287V, 287W, 287Y, 292A, 292C, 292D, 292E, 292F, 292G, 292H, 292I, 292K, 292M, 292N, 292P, 292Q, 292R, 292S, 292T, 292V, 292W, 292Y, 293A, 293C, 293D, 293E, 293F, 293G, 293H, 293I, 293K, 293L, 293M, 293N, 293P, 293Q, 293S, 293T, 293V, 293W, 293Y, 296A, 296C, 296D, 296E, 296F, 296G, 296H, 296I, 296K, 296L, 296M, 296N, 296P, 296Q, 296S, 296T, 296V, 296W, 296Y, 303A, 303C, 303D, 303E, 303F, 303G, 303H, 303I, 303K, 303L, 303M, 303N, 303Q, 303R, 303S, 303T, 303V, 303W, 303Y, 334A, 334C, 334D, 334E, 334F, 334G, 334H, 334I, 334K, 334L, 334M, 334N, 334P, 334Q, 334R, 334S, 334V, 334W, 334Y, 344A, 344C, 344D, 344E, 344F, 344G, 344I, 344K, 344L, 344M, 344N, 344P, 344Q, 344R, 344S, 344T, 344V, 344W, 344Y, 373A, 373C, 373D, 373E, 373G, 373H, 373I, 373K, 373L, 373M, 373N, 373P, 373Q, 373R, 373S, 373T, 373V, 373W, 373Y, 385A, 385C, 385D, 385E, 385F, 385G, 385H, 385I, 385K, 385L, 385M, 385N, 385Q, 385R, 385S, 385T, 385V, 385W, 및 385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 추가의 실시양태에서, 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 재조합 리파제는 M24A, M24C, M24D, M24E, M24F, M24F/G38F/T49V/H70K/E149T/I161L/R174E/L175Q/A189S/L193Q/L225M/E231Q/R243L/V252M/D271G/L287F/L292M/R293Q/R296S/P303A/T334D/H344S/F373Y/P385F, M24G, M24H, M24I, M24K, M24L, M24N, M24P, M24Q, M24R, M24S, M24T, M24V, M24W, M24Y, G38A, G38C, G38D, G38E, G38F, G38H, G38I, G38K, G38L, G38M, G38N, G38P, G38Q, G38R, G38S, G38T, G38V, G38W, G38Y, T49A, T49C, T49D, T49E, T49F, T49G, T49H, T49I, T49K, T49L, T49M, T49N, T49P, T49Q, T49R, T49S, T49V, T49W, T49Y, H70A, H70C, H70D, H70E, H70F, H70G, H70I, H70K, H70L, H70M, H70N, H70P, H70Q, H70R, H70S, H70T, H70V, H70W, H70Y, E149A, E149C, E149D, E149F, E149G, E149H, E149I, E149K, E149L, E149M, E149N, E149P, E149Q, E149R, E149S, E149T, E149V, E149W, E149Y, I161A, I161C, I161D, I161E, I161F, I161G, I161H, I161K, I161L, I161M, I161N, I161P, I161Q, I161R, I161S, I161T, I161V, I161W, I161Y, R174A, R174C, R174D, R174E, R174F, R174G, R174H, R174I, R174K, R174L, R174M, R174N, R174P, R174Q, R174S, R174T, R174V, R174W, R174Y, L175A, L175C, L175D, L175E, L175F, L175G, L175H, L175I, L175K, L175M, L175N, L175P, L175Q, L175R, L175S, L175T, L175V, L175W, L175Y, A189C, A189D, A189E, A189F, A189G, A189H, A189I, A189K, A189L, A189M, A189N, A189P, A189Q, A189R, A189S, A189T, A189V, A189W, A189Y, L193A, L193C, L193D, L193E, L193F, L193G, L193H, L193I, L193K, L193M, L193N, L193P, L193Q, L193R, L193S, L193T, L193V, L193W, L193Y, L225A, L225C, L225D, L225E, L225F, L225G, L225H, L225I, L225K, L225M, L225N, L225P, L225Q, L225R, L225S, L225T, L225V, L225W, L225Y, E231A, E231C, E231D, E231F, E231G, E231H, E231I, E231K, E231L, E231M, E231N, E231P, E231Q, E231R, E231S, E231T, E231V, E231W, E231Y, R243A, R243C, R243D, R243E, R243F, R243G, R243H, R243I, R243K, R243L, R243M, R243N, R243P, R243Q, R243S, R243T, R243V, R243W, R243Y, V252A, V252C, V252D, V252E, V252F, V252G, V252H, V252I, V252K, V252L, V252M, V252N, V252P, V252Q, V252R, V252S, V252T, V252W, V252Y, D271A, D271C, D271E, D271F, D271G, D271H, D271I, D271K, D271L, D271M, D271N, D271P, D271Q, D271R, D271S, D271T, D271V, D271W, D271Y, L287A, L287C, L287D, L287E, L287F, L287G, L287H, L287I, L287K, L287M, L287N, L287P, L287Q, L287R, L287S, L287T, L287V, L287W, L287Y, L292A, L292C, L292D, L292E, L292F, L292G, L292H, L292I, L292K, L292M, L292N, L292P, L292Q, L292R, L292S, L292T, L292V, L292W, L292Y, R293A, R293C, R293D, R293E, R293F, R293G, R293H, R293I, R293K, R293L, R293M, R293N, R293P, R293Q, R293S, R293T, R293V, R293W, R293Y, R296A, R296C, R296D, R296E, R296F, R296G, R296H, R296I, R296K, R296L, R296M, R296N, R296P, R296Q, R296S, R296T, R296V, R296W, R296Y, P303A, P303C, P303D, P303E, P303F, P303G, P303H, P303I, P303K, P303L, P303M, P303N, P303Q, P303R, P303S, P303T, P303V, P303W, P303Y, T334A, T334C, T334D, T334E, T334F, T334G, T334H, T334I, T334K, T334L, T334M, T334N, T334P, T334Q, T334R, T334S, T334V, T334W, T334Y, H344A, H344C, H344D, H344E, H344F, H344G, H344I, H344K, H344L, H344M, H344N, H344P, H344Q, H344R, H344S, H344T, H344V, H344W, H344Y, F373A, F373C, F373D, F373E, F373G, F373H, F373I, F373K, F373L, F373M, F373N, F373P, F373Q, F373R, F373S, F373T, F373V, F373W, F373Y, P385A, P385C, P385D, P385E, P385F, P385G, P385H, P385I, P385K, P385L, P385M, P385N, P385Q, P385R, P385S, P385T, P385V, P385W, 및 P385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다.
[0119]
일부 실시양태에서, 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868에 대해 적어도 70%, 75%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868를 참조하여 번호가 매겨진다.
일부 실시양태에서, 재조합 리파제 폴리펩티드 서열은 서열번호 14-1796의 짝수 번호 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 본 발명에 의해 포괄되는 조작된 리파제 폴리펩티드의 기능성 단편을 포함한다. 기능성 단편은 이것이 유도되는 조작된 리파제 폴리펩티드(즉, 모 조작된 리파제)의 활성의 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%를 갖는다. 일부 실시양태에서, 기능성 단편은 조작된 리파제의 모 서열의 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 및 심지어 99%를 포함한다. 일부 실시양태에서, 기능성 단편은 5개 미만, 10개 미만, 15개 미만, 10개 미만, 25개 미만, 30개 미만, 35개 미만, 40개 미만, 45개 미만, 및 50개 미만의 아미노산이 말단절단된다.
조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드, 발현 벡터 및 숙주 세포:
본 발명은 본원에 기재된 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드를 제공한다. 일부 실시양태에서, 폴리뉴클레오티드는 폴리펩티드를 발현할 수 있는 재조합 폴리뉴클레오티드를 생성하기 위해 유전자 발현을 제어하는 하나 이상의 이종 조절 서열에 작동 가능하게 연결된다. 조작된 리파제 폴리펩티드를 코딩하는 이종 폴리뉴클레오티드를 함유하는 발현 구축물은 상응하는 리파제 폴리펩티드를 발현하기 위해 적절한 숙주 세포로 도입될 수 있다.
당업자에게 명백한 바와 같이, 단백질 서열의 이용가능성 및 다양한 아미노산에 상응하는 코돈의 지식은 대상 폴리펩티드를 코딩할 수 있는 모든 폴리뉴클레오티드의 설명을 제공한다. 동일한 아미노산이 대체 또는 동의적 코돈에 의해 코딩되는 유전 암호의 축퇴성은 매우 많은 수의 핵산이 만들어지도록 하며, 이들 모두는 조작된 리파제 폴리펩티드를 코딩한다. 따라서, 특정 아미노산 서열에 대한 지식이 있는 당업자는 단백질의 아미노산 서열을 변경하지 않는 방식으로 하나 이상의 코돈의 서열을 단순히 변형함으로써 임의의 수의 상이한 핵산을 만들 수 있다. 이와 관련하여, 본 발명은 가능한 코돈 선택에 기초하여 조합을 선택함으로써 본원에 기재된 폴리펩티드를 코딩할 수 있는 폴리뉴클레오티드의 각각 및 모든 가능한 변이를 구체적으로 고려하고, 이러한 모든 변이는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7 및 5-1에서 제공되는 변형을 포함하여 본원에 기재된 임의의 폴리펩티드에 대해 구체적으로 개시된 것으로 간주되어야 한다.
다양한 실시양태에서, 코돈은 바람직하게는 단백질이 생성되는 숙주 세포에 맞도록 선택된다. 예컨대, 박테리아에서 사용되는 선호되는 코돈은 박테리아에서의 발현에 사용되는 반면, 진균에서 사용되는 선호되는 코돈은 진균에서의 발현에 사용된다. 결과적으로, 조작된 리파제 폴리펩티드를 코딩하는 코돈 최적화된 폴리뉴클레오티드는 전장 코딩 영역의 코돈 위치의 약 40%, 50%, 60%, 70%, 80%, 또는 90% 초과에서 바람직한 코돈을 함유한다.
일부 실시양태에서, 본 발명은 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867에 대해 적어도 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99%, 또는 그 초과의 서열 동일성을 갖는 재조합 폴리뉴클레오티드 서열을 제공한다. 일부 실시양태에서, 본 발명은 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 재조합 폴리뉴클레오티드 서열을 제공한다. 일부 실시양태에서, 재조합 폴리뉴클레오티드 서열은 서열번호 13-1795의 홀수 번호 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 2, 3, 4, 22, 27/46/97/136/149/385, 27/46/385, 27/70/136/231/385, 27/136/323/385, 27/149, 27/149/385, 27/385, 34, 38, 41, 44, 46/70, 46/149/183/231/385, 48, 70, 73, 73/305, 82, 82/94/101/194/199, 82/94/199, 83, 85, 87, 89, 92, 94, 96, 97/149/385, 135, 136/385, 140, 141, 142, 144, 146, 149, 149/231/385, 151, 174, 175, 178, 181, 183/385, 189, 194, 194/199, 195, 195/231/385, 199, 199/213, 210, 212, 213, 213/330, 216, 218, 219, 226, 231/385, 238, 247, 250, 270, 274, 281, 292, 296, 300, 308, 330, 338, 379, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 2H, 2M, 2T, 3A, 3G, 3R, 3S, 4M, 4W, 4Y, 22N, 27V/46T/97A/136V/149E/385P, 27V/46T/385P, 27V/70N/136V/231E/385P, 27V/136V/323I/385P, 27V/149E, 27V/149E/385P, 27V/385P, 34D, 38A, 38H, 38L, 41V, 44E, 46T/70N, 46T/149E/183L/231E/385P, 48V, 70N, 73C/305I, 73I, 73R, 82C, 82E, 82E/94S/101S/194N/199L, 82E/94S/199L, 82F, 82G, 82L, 83G, 83K, 85I, 85W, 87R, 89A, 89T, 89V, 89W, 92A, 94S, 96E, 96N, 96S, 97A/149E/385P, 135G, 135Q, 135S, 135T, 135V, 136V/385P, 140T, 141A, 141F, 141L, 141S, 142A, 142F, 142I, 142L, 144M, 144R, 144W, 146A, 146F, 146S, 146Y, 149E, 149E/231E/385P, 149K, 149R, 151A, 151I, 151L, 174H, 174R, 175G, 175L, 175R, 178R, 178T, 181H, 183L/385P, 189A, 189E, 189W, 194N, 194N/199L, 194T, 195D/231E/385P, 195V, 199L, 199L/213A, 210A, 210V, 212C, 212G, 212R, 212T, 213A/330T, 213H, 216R, 216T, 216W, 218D, 218G, 218I, 218M, 218T, 219C, 219G, 219K, 219R, 226R, 231E/385P, 238S, 247R, 250T, 270V, 274D, 281K, 281R, 292A, 292C, 292L, 292V, 296M, 296R, 300A, 300D, 300T, 308A, 330Y, 338N, 379T, 385A, 385C, 385D, 385P, 385R, 및 385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 E2H, E2M, E2T, T3A, T3G, T3R, T3S, S4M, S4W, S4Y, G22N, Y27V/R46T/Y97A/Y136V/T149E/F385P, Y27V/R46T/F385P, Y27V/K70N/Y136V/Q231E/F385P, Y27V/Y136V/K323I/F385P, Y27V/T149E, Y27V/T149E/F385P, Y27V/F385P, K34D, F38A, F38H, F38L, N41V, G44E, R46T/K70N, R46T/T149E/F183L/Q231E/F385P, Y48V, K70N, T73C/V305I, T73I, T73R, K82C, K82E, K82E/P94S/D101S/K194N/I199L, K82E/P94S/I199L, K82F, K82G, K82L, E83G, E83K, G85I, G85W, A87R, F89A, F89T, F89V, F89W, T92A, P94S, I96E, I96N, I96S, Y97A/T149E/F385P, Y135G, Y135Q, Y135S, Y135T, Y135V, Y136V/F385P, P140T, E141A, E141F, E141L, E141S, E142A, E142F, E142I, E142L, I144M, I144R, I144W, P146A, P146F, P146S, P146Y, T149E, T149E/Q231E/F385P, T149K, T149R, G151A, G151I, G151L, E174H, E174R, Q175G, Q175L, Q175R, S178R, S178T, K181H, F183L/F385P, S189A, S189E, S189W, K194N, K194N/I199L, K194T, Q195D/Q231E/F385P, Q195V, I199L, I199L/P213A, K210A, K210V, Q212C, Q212G, Q212R, Q212T, P213A/N330T, P213H, S216R, S216T, S216W, H218D, H218G, H218I, H218M, H218T, A219C, A219G, A219K, A219R, T226R, Q231E/F385P, Y238S, E247R, Q250T, R270V, T274D, G281K, G281R, M292A, M292C, M292L, M292V, S296M, S296R, Q300A, Q300D, Q300T, R308A, N330Y, K338N, N379T, F385A, F385C, F385D, F385P, F385R, 및 F385T로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 3/4/96/296/300, 3/292/296, 4, 4/11/149/292/296/300, 4/96/149/231/296/300, 4/96/149/292/296/300, 4/96/219/296, 4/96/231/296/300, 4/96/292/296/300, 4/149/174, 4/174/219/292/296, 4/231/296/300, 27, 27/34/82, 27/73/82/218, 27/89, 27/89/178, 27/89/218, 27/218, 34/73/218, 34/82, 34/218, 73/82, 73/82/183, 94/146/175, 96/149/174/292/296, 96/149/231/292/296, 96/149/292/296, 96/174/219/231/292/296, 96/231/296, 146/175/189/281, 149/174/292/296, 149/174/300, 149/219/231/292/296/300, 149/231/292/296, 149/231/292/296/300, 149/296, 149/296/300, 174/231, 174/296, 218, 231, 231/292/296, 231/292/296/300, 231/296, 231/300, 292/296, 292/296/300, 296, 및 296/300으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 3S/4W/96E/296R/300D, 3S/292L/296R, 4W, 4W/11A/149E/292L/296R/300D, 4W/96E/149E/231E/296R/300D, 4W/96E/149E/292L/296R/300D, 4W/96E/219K/296R, 4W/96E/231E/296R/300D, 4W/96E/292L/296R/300D, 4W/149E/174R, 4W/174H/219K/292L/296R, 4W/231E/296R/300D, 27V, 27V/34D/82E, 27V/73I/82L/218I, 27V/73I/82L/218M, 27V/89T, 27V/89T/178P, 27V/89T/218I, 27V/218I, 34D/73I/218I, 34D/82C, 34D/218M, 73I/82C, 73I/82L/183L, 94S/146F/175G, 96E/149E/174H/292L/296R, 96E/149E/231E/292L/296R, 96E/149E/292L/296R, 96E/174H/219K/231E/292L/296R, 96E/231E/296R, 146F/175G/189A/281K, 149E/174H/292L/296R, 149E/174H/300D, 149E/219K/231E/292L/296R/300D, 149E/231E/292L/296R, 149E/231E/292L/296R/300D, 149E/296R, 149E/296R/300D, 174H/296R, 174R/231E, 218I, 231E, 231E/292L/296R, 231E/292L/296R/300D, 231E/296R, 231E/300D, 292L/296R, 292L/296R/300D, 296R, 및 296R/300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 T3S/S4W/I96E/S296R/Q300D, T3S/M292L/S296R, S4W, S4W/V11A/T149E/M292L/S296R/Q300D, S4W/I96E/T149E/Q231E/S296R/Q300D, S4W/I96E/T149E/M292L/S296R/Q300D, S4W/I96E/A219K/S296R, S4W/I96E/Q231E/S296R/Q300D, S4W/I96E/M292L/S296R/Q300D, S4W/T149E/E174R, S4W/E174H/A219K/M292L/S296R, S4W/Q231E/S296R/Q300D, Y27V, Y27V/K34D/K82E, Y27V/T73I/K82L/H218I, Y27V/T73I/K82L/H218M, Y27V/F89T, Y27V/F89T/S178P, Y27V/F89T/H218I, Y27V/H218I, K34D/T73I/H218I, K34D/K82C, K34D/H218M, T73I/K82C, T73I/K82L/F183L, P94S/P146F/Q175G, I96E/T149E/E174H/M292L/S296R, I96E/T149E/Q231E/M292L/S296R, I96E/T149E/M292L/S296R, I96E/E174H/A219K/Q231E/M292L/S296R, I96E/Q231E/S296R, P146F/Q175G/S189A/G281K, T149E/E174H/M292L/S296R, T149E/E174H/Q300D, T149E/A219K/Q231E/M292L/S296R/Q300D, T149E/Q231E/M292L/S296R, T149E/Q231E/M292L/S296R/Q300D, T149E/S296R, T149E/S296R/Q300D, E174H/S296R, E174R/Q231E, H218I, Q231E, Q231E/M292L/S296R, Q231E/M292L/S296R/Q300D, Q231E/S296R, Q231E/Q300D, M292L/S296R, M292L/S296R/Q300D, S296R, 및 S296R/Q300D로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 4, 4/27/189/300, 4/70, 4/102, 4/137, 4/175/189/218, 4/175/189/300, 4/175/218/224, 4/175/218/373, 4/185, 4/189/218/373, 4/193, 4/218/300/369/373, 4/228, 4/233, 4/243, 4/271, 4/303, 4/334, 4/336, 4/375, 25, 27/82/174/175/189/218/300/369/373, 27/82/174/175/218/300/369, 27/82/174/175/218/300/373/382, 27/82/174/218/300/373, 27/189/218/373, 28, 33, 99, 102, 104, 134, 153, 174/175/189, 174/373, 175/189/218/300/373, 175/189/373, 175/218, 175/218/382, 189/218/300/373, 191, 193, 231, 233, 243, 293, 303, 331, 336, 339, 368, 및 373으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 4W, 4W/27V/189A/300D, 4W/70H, 4W/102T, 4W/137R, 4W/175G/189A/218M, 4W/175G/189A/300D, 4W/175G/218M/224A, 4W/175G/218M/373F, 4W/185L, 4W/189A/218M/373F, 4W/193T, 4W/218M/300D/369N/373F, 4W/228E, 4W/233R, 4W/243R, 4W/271D, 4W/303P, 4W/334T, 4W/336S, 4W/336T, 4W/375A, 25V, 27V/82C/174H/175G/189A/218M/300D/369N/373F, 27V/82C/174H/175G/218M/300D/369N, 27V/82C/174H/175G/218M/300D/373F/382M, 27V/82C/174H/218M/300D/373F, 27V/189A/218M/373F, 28N, 33Y, 99D, 102L, 104H, 134R, 153G, 174H/175L/189A, 174H/373F, 175G/189A/218M/300D/373F, 175G/218M, 175G/218M/382M, 175L/189A/373F, 189A/218M/300D/373F, 191L, 193L, 231Q, 233G, 233S, 233W, 243R, 293R, 303P, 331R, 336R, 339K, 368A, 368T, 및 373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 S4W, S4W/Y27V/S189A/Q300D, S4W/K70H, S4W/E102T, S4W/S137R, S4W/Q175G/S189A/H218M, S4W/Q175G/S189A/Q300D, S4W/Q175G/H218M/V224A, S4W/Q175G/H218M/Y373F, S4W/I185L, S4W/S189A/H218M/Y373F, S4W/Q193T, S4W/H218M/Q300D/S369N/Y373F, S4W/P228E, S4W/N233R, S4W/L243R, S4W/G271D, S4W/A303P, S4W/D334T, S4W/A336S, S4W/A336T, S4W/N375A, L25V, Y27V/K82C/E174H/Q175G/S189A/H218M/Q300D/S369N/Y373F, Y27V/K82C/E174H/Q175G/H218M/Q300D/S369N, Y27V/K82C/E174H/Q175G/H218M/Q300D/Y373F/H382M, Y27V/K82C/E174H/H218M/Q300D/Y373F, Y27V/S189A/H218M/Y373F, R28N, L33Y, Q99D, E102L, N104H, E134R, N153G, E174H/Q175L/S189A, E174H/Y373F, Q175G/S189A/H218M/Q300D/Y373F, Q175G/H218M, Q175G/H218M/H382M, Q175L/S189A/Y373F, S189A/H218M/Q300D/Y373F, A191L, Q193L, E231Q, N233G, N233S, N233W, L243R, Q293R, A303P, S331R, A336R, Q339K, F368A, F368T, 및 Y373F로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 26, 33, 33/174/193/243, 33/174/334, 33/175/218/303, 33/175/218/334/339, 33/193, 34, 38, 46, 48, 70/271/293/334, 144, 174, 174/175/193, 174/175/218/339, 174/193/303/375, 174/193/375, 174/218/233/271/293/303, 174/218/271/303, 175/193/218/233/243/375, 175/193/218/243, 175/218/375, 181, 189, 193, 193/218/243, 193/271/303/334, 193/293/303, 194, 195, 199, 210, 218, 218/243, 218/243/303/334, 218/303, 225, 238, 274, 281, 및 330으로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 G26A, G26R, G26S, L33Y, L33Y/E174H/Q193L/L243R, L33Y/E174H/D334T, L33Y/L175G/H218M/A303P, L33Y/L175G/H218M/D334T/Q339K, L33Y/Q193L, K34L, F38A, F38G, R46P, Y48L, K70H/G271D/Q293R/D334T, I144L, E174H, E174H/L175G/Q193L, E174H/L175G/H218M/Q339K, E174H/Q193L/A303P/N375A, E174H/Q193L/N375A, E174H/H218M/N233R/G271D/Q293R/A303P, E174H/H218M/G271D/A303P, E174R, L175G/Q193L/H218M/N233R/L243R/N375A, L175G/Q193L/H218M/L243R, L175G/H218M/N375A, K181Q, A189H, Q193L, Q193L/H218M/L243R, Q193L/G271D/A303P/D334T, Q193L/Q293R/A303P, K194T, Q195I, Q195L, Q195Y, I199H, K210V, H218C, H218D, H218M, H218M/L243R, H218M/L243R/A303P/D334T, H218M/A303P, H218P, M225L, Y238W, T274D, G281K, G281P, N330F, 및 N330H로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 G26A, G26R, G26S, L33Y, L33Y/E174H/Q193L/L243R, L33Y/E174H/D334T, L33Y/L175G/H218M/A303P, L33Y/L175G/H218M/D334T/Q339K, L33Y/Q193L, K34L, F38A, F38G, R46P, Y48L, K70H/G271D/Q293R/D334T, I144L, E174H, E174H/L175G/Q193L, E174H/L175G/H218M/Q339K, E174H/Q193L/A303P/N375A, E174H/Q193L/N375A, E174H/H218M/N233R/G271D/Q293R/A303P, E174H/H218M/G271D/A303P, E174R, L175G/Q193L/H218M/N233R/L243R/N375A, L175G/Q193L/H218M/L243R, L175G/H218M/N375A, K181Q, A189H, Q193L, Q193L/H218M/L243R, Q193L/G271D/A303P/D334T, Q193L/Q293R/A303P, K194T, Q195I, Q195L, Q195Y, I199H, K210V, H218C, H218D, H218M, H218M/L243R, H218M/L243R/A303P/D334T, H218M/A303P, H218P, M225L, Y238W, T274D, G281K, G281P, N330F, 및 N330H로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 34/38/174/193/195/243/281/303/330, 34/38/174/225/303, 34/38/174/303/345, 34/174, 34/174/193/195/243/281, 34/174/193/195/303/330, 34/193/195/225/243, 34/193/243/303/330, 34/281/330, 38, 38/174/193/195/243/330, 38/174/193/225/274/283/303/330, 38/174/193/303, 38/174/195/281/330, 38/174/225/243/281/330, 38/174/281, 38/174/281/303, 38/174/281/303/345, 38/193/195/225/303/345, 38/195/243/303, 38/195/281/303/330, 38/195/281/330, 38/195/303, 49, 49/51/98/252/311, 49/51/123/252/344, 49/98/120/252/344, 49/120/252/344, 49/123/252/344, 49/123/264/344, 49/123/311, 49/252/344, 49/311/344, 49/344, 51, 51/252/344, 51/344, 98, 98/344, 123/252/344, 129, 160, 161, 174/193/195/225, 174/193/303/330, 174/195/225/281/303/330/345, 174/195/243/281/345, 174/195/281/303, 174/225/243/281/303/345, 174/281/330, 174/303, 195/225/303/330, 252, 268, 및 344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 34L/38A/174G/193L/195L/243R/281P/303P/330F, 34L/38A/174R/303P/345I, 34L/38G/174R/225L/303P, 34L/174G, 34L/174G/193L/195L/243R/281P, 34L/174H/193L/195I/303P/330F, 34L/193L/195I/225L/243R, 34L/193L/243R/303P/330F, 34L/281P/330H, 38A/174H/193L/225L/274D/283I/303P/330F, 38A/174R/281K/303P/345I, 38A/193L/195L/225L/303P/345I, 38A/195I/303P, 38A/195Y/281K/303P/330F, 38G, 38G/174G/193L/303P, 38G/174G/281K/303P, 38G/174H/195L/281P/330F, 38G/174H/225L/243R/281K/330H, 38G/174H/281K, 38G/174R/193L/195L/243R/330H, 38G/195I/281P/330F, 38G/195Y/243R/303P, 49S, 49T, 49T/51A/98P/252V/311W, 49T/51A/123Q/252V/344H, 49T/98P/120T/252V/344H, 49T/120T/252V/344H, 49T/123Q/252V/344H, 49T/123Q/264S/344H, 49T/123Q/311W, 49T/252V/344H, 49T/311W/344H, 49T/344H, 51A/252V/344H, 51A/344H, 51V, 98P/344H, 98R, 123Q/252V/344H, 129F, 160T, 161I, 174G/195I/225L/281K/303P/330F/345I, 174G/225L/243R/281P/303P/345I, 174H/195I/243R/281K/345I, 174H/281P/330H, 174R/193L/195I/225L, 174R/193L/303P/330H, 174R/195I/281K/303P, 174R/303P, 195Y/225L/303P/330H, 252V, 268T, 344I, 344V, 및 344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 K34L/F38A/E174G/Q193L/Q195L/L243R/G281P/A303P/N330F, K34L/F38A/E174R/A303P/F345I, K34L/F38G/E174R/M225L/A303P, K34L/E174G, K34L/E174G/Q193L/Q195L/L243R/G281P, K34L/E174H/Q193L/Q195I/A303P/N330F, K34L/Q193L/Q195I/M225L/L243R, K34L/Q193L/L243R/A303P/N330F, K34L/G281P/N330H, F38A/E174H/Q193L/M225L/T274D/M283I/A303P/N330F, F38A/E174R/G281K/A303P/F345I, F38A/Q193L/Q195L/M225L/A303P/F345I, F38A/Q195I/A303P, F38A/Q195Y/G281K/A303P/N330F, F38G, F38G/E174G/Q193L/A303P, F38G/E174G/G281K/A303P, F38G/E174H/Q195L/G281P/N330F, F38G/E174H/M225L/L243R/G281K/N330H, F38G/E174H/G281K, F38G/E174R/Q193L/Q195L/L243R/N330H, F38G/Q195I/G281P/N330F, F38G/Q195Y/L243R/A303P, V49S, V49T, V49T/T51A/G98P/M252V/L311W, V49T/T51A/E123Q/M252V/S344H, V49T/G98P/M120T/M252V/S344H, V49T/M120T/M252V/S344H, V49T/E123Q/M252V/S344H, V49T/E123Q/T264S/S344H, V49T/E123Q/L311W, V49T/M252V/S344H, V49T/L311W/S344H, V49T/S344H, T51A/M252V/S344H, T51A/S344H, T51V, G98P/S344H, G98R, E123Q/M252V/S344H, S129F, S160T, L161I, E174G/Q195I/M225L/G281K/A303P/N330F/F345I, E174G/M225L/L243R/G281P/A303P/F345I, E174H/Q195I/L243R/G281K/F345I, E174H/G281P/N330H, E174R/Q193L/Q195I/M225L, E174R/Q193L/A303P/N330H, E174R/Q195I/G281K/A303P, E174R/A303P, Q195Y/M225L/A303P/N330H, M252V, S268T, S344I, S344V, 및 S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 11/16/67/168/180/287, 11/16/237/241/287, 11/67/168/287, 16/67/154/168/237/241/287, 16/67/237/287/291, 16/168/177, 24, 24/278, 34/49/161/193/243/344, 34/49/161/193/344, 34/49/161/243/344, 34/49/161/252/268/344, 34/49/193/243/252/344, 34/49/193/344, 34/49/243/252, 34/49/252/268/344, 34/161/193/243/252/268, 34/161/193/243/268/344, 34/161/193/243/344, 34/161/193/252/268/344, 34/161/193/252/344, 34/161/193/268/344, 34/161/243/281/344, 34/161/344, 34/193/243/252/344, 34/193/252/268/344, 34/193/268/281, 34/252/268/281/344, 34/252/344, 49/161/193/243/252/344, 49/161/193/252/281/344, 49/161/243/252/344, 49/193/197/243/252/281/344, 49/193/243/252/344, 49/193/243/344, 49/243/344, 67/154/237/287, 67/168/237, 67/168/237/287, 67/177/237, 154/237/286/287, 154/286/287, 161/193/252/344, 161/193/344, 161/344, 168/237, 186/187/278, 193/243/281/344, 193/252/268/344, 193/252/281/344, 237, 237/287/291, 및 281/344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 11I/16F/67A/168T/180V/287L, 11I/16F/237Q/241S/287L, 11I/67A/168T/287L, 16F/67A/154Y/168T/237Q/241S/287L, 16F/67A/237Q/287L/291A, 16F/168T/177L, 24M, 24M/278L, 34K/49T/161I/193L/243R/344H, 34K/49T/161I/193L/243R/344V, 34K/49T/161I/193L/344W, 34K/49T/161I/243R/344H, 34K/49T/161I/252V/268T/344V, 34K/49T/193L/243R/252V/344W, 34K/49T/193L/344H, 34K/49T/193L/344W, 34K/49T/243R/252V, 34K/49T/252V/268T/344V, 34K/161I/193L/243R/252V/268T, 34K/161I/193L/243R/268T/344V, 34K/161I/193L/243R/344W, 34K/161I/193L/252V/268T/344H, 34K/161I/193L/252V/268T/344V, 34K/161I/193L/252V/344H, 34K/161I/193L/252V/344V, 34K/161I/193L/252V/344W, 34K/161I/193L/268T/344H, 34K/161I/243R/281P/344V, 34K/161I/344V, 34K/161I/344W, 34K/193L/243R/252V/344W, 34K/193L/252V/268T/344H, 34K/193L/268T/281P, 34K/252V/268T/281P/344V, 34K/252V/344V, 49T/161I/193L/243R/252V/344W, 49T/161I/193L/252V/281P/344H, 49T/161I/243R/252V/344V, 49T/193L/197G/243R/252V/281P/344H, 49T/193L/243R/252V/344V, 49T/193L/243R/344W, 49T/243R/344V, 67A/154Y/237Q/287L, 67A/168T/237Q, 67A/168T/237Q/287L, 67A/177L/237Q, 154Y/237Q/286V/287L, 154Y/286V/287L, 161I/193L/252V/344V, 161I/193L/344W, 161I/344W, 168T/237Q, 186T/187A/278L, 193L/243R/281P/344W, 193L/252V/268T/344V, 193L/252V/281P/344H, 237Q, 237Q/287L/291A, 및 281P/344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 V11I/L16F/Y67A/S168T/I180V/F287L, V11I/L16F/A237Q/T241S/F287L, V11I/Y67A/S168T/F287L, L16F/Y67A/W154Y/S168T/A237Q/T241S/F287L, L16F/Y67A/A237Q/F287L/S291A, L16F/S168T/V177L, F24M, F24M/Y278L, L34K/V49T/L161I/Q193L/L243R/S344H, L34K/V49T/L161I/Q193L/L243R/S344V, L34K/V49T/L161I/Q193L/S344W, L34K/V49T/L161I/L243R/S344H, L34K/V49T/L161I/M252V/S268T/S344V, L34K/V49T/Q193L/L243R/M252V/S344W, L34K/V49T/Q193L/S344H, L34K/V49T/Q193L/S344W, L34K/V49T/L243R/M252V, L34K/V49T/M252V/S268T/S344V, L34K/L161I/Q193L/L243R/M252V/S268T, L34K/L161I/Q193L/L243R/S268T/S344V, L34K/L161I/Q193L/L243R/S344W, L34K/L161I/Q193L/M252V/S268T/S344H, L34K/L161I/Q193L/M252V/S268T/S344V, L34K/L161I/Q193L/M252V/S344H, L34K/L161I/Q193L/M252V/S344V, L34K/L161I/Q193L/M252V/S344W, L34K/L161I/Q193L/S268T/S344H, L34K/L161I/L243R/G281P/S344V, L34K/L161I/S344V, L34K/L161I/S344W, L34K/Q193L/L243R/M252V/S344W, L34K/Q193L/M252V/S268T/S344H, L34K/Q193L/S268T/G281P, L34K/M252V/S268T/G281P/S344V, L34K/M252V/S344V, V49T/L161I/Q193L/L243R/M252V/S344W, V49T/L161I/Q193L/M252V/G281P/S344H, V49T/L161I/L243R/M252V/S344V, V49T/Q193L/S197G/L243R/M252V/G281P/S344H, V49T/Q193L/L243R/M252V/S344V, V49T/Q193L/L243R/S344W, V49T/L243R/S344V, Y67A/W154Y/A237Q/F287L, Y67A/S168T/A237Q, Y67A/S168T/A237Q/F287L, Y67A/V177L/A237Q, W154Y/A237Q/L286V/F287L, W154Y/L286V/F287L, L161I/Q193L/M252V/S344V, L161I/Q193L/S344W, L161I/S344W, S168T/A237Q, H186T/L187A/Y278L, Q193L/L243R/G281P/S344W, Q193L/M252V/S268T/S344V, Q193L/M252V/G281P/S344H, A237Q, A237Q/F287L/S291A, 및 G281P/S344W로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 24/168/252/281, 24/237/287/344, 24/252, 24/252/287, 24/344, 213/252/278/344, 252/278/344, 252/287/344, 및 281로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 24M/168T/252V/281P, 24M/237Q/287L/344S, 24M/252V, 24M/252V/287L, 24M/344W, 213S/252V/278L/344S, 252V/278L/344W, 252V/287L/344S, 및 281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 F24M/S168T/M252V/G281P, F24M/A237Q/F287L/H344S, F24M/M252V, F24M/M252V/F287L, F24M/H344W, P213S/M252V/Y278L/H344S, M252V/Y278L/H344W, M252V/F287L/H344S, 및 G281P로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨진다.
일부 실시양태에서, 폴리뉴클레오티드는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 24, 24/38/49/70/149/161/174/175/189/193/225/231/243/252/271/287/292/293/296/303/334/344/373/385, 38, 49, 70, 149, 161, 174, 175, 189, 193, 225, 231, 243, 252, 271, 287, 292, 293, 296, 303, 334, 344, 373, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 24A, 24C, 24D, 24E, 24F, 24F/38F/49V/70K/149T/161L/174E/175Q/189S/193Q/225M/231Q/243L/252M/271G/287F/292M/293Q/296S/303A/334D/344S/373Y/385F, 24G, 24H, 24I, 24K, 24L, 24N, 24P, 24Q, 24R, 24S, 24T, 24V, 24W, 24Y, 38A, 38C, 38D, 38E, 38F, 38H, 38I, 38K, 38L, 38M, 38N, 38P, 38Q, 38R, 38S, 38T, 38V, 38W, 38Y, 49A, 49C, 49D, 49E, 49F, 49G, 49H, 49I, 49K, 49L, 49M, 49N, 49P, 49Q, 49R, 49S, 49V, 49W, 49Y, 70A, 70C, 70D, 70E, 70F, 70G, 70I, 70K, 70L, 70M, 70N, 70P, 70Q, 70R, 70S, 70T, 70V, 70W, 70Y, 149A, 149C, 149D, 149F, 149G, 149H, 149I, 149K, 149L, 149M, 149N, 149P, 149Q, 149R, 149S, 149T, 149V, 149W, 149Y, 161A, 161C, 161D, 161E, 161F, 161G, 161H, 161K, 161L, 161M, 161N, 161P, 161Q, 161R, 161S, 161T, 161V, 161W, 161Y, 174A, 174C, 174D, 174E, 174F, 174G, 174H, 174I, 174K, 174L, 174M, 174N, 174P, 174Q, 174S, 174T, 174V, 174W, 174Y, 175A, 175C, 175D, 175E, 175F, 175G, 175H, 175I, 175K, 175M, 175N, 175P, 175Q, 175R, 175S, 175T, 175V, 175W, 175Y, 189C, 189D, 189E, 189F, 189G, 189H, 189I, 189K, 189L, 189M, 189N, 189P, 189Q, 189R, 189S, 189T, 189V, 189W, 189Y, 193A, 193C, 193D, 193E, 193F, 193G, 193H, 193I, 193K, 193M, 193N, 193P, 193Q, 193R, 193S, 193T, 193V, 193W, 193Y, 225A, 225C, 225D, 225E, 225F, 225G, 225H, 225I, 225K, 225M, 225N, 225P, 225Q, 225R, 225S, 225T, 225V, 225W, 225Y, 231A, 231C, 231D, 231F, 231G, 231H, 231I, 231K, 231L, 231M, 231N, 231P, 231Q, 231R, 231S, 231T, 231V, 231W, 231Y, 243A, 243C, 243D, 243E, 243F, 243G, 243H, 243I, 243K, 243L, 243M, 243N, 243P, 243Q, 243S, 243T, 243V, 243W, 243Y, 252A, 252C, 252D, 252E, 252F, 252G, 252H, 252I, 252K, 252L, 252M, 252N, 252P, 252Q, 252R, 252S, 252T, 252W, 252Y, 271A, 271C, 271E, 271F, 271G, 271H, 271I, 271K, 271L, 271M, 271N, 271P, 271Q, 271R, 271S, 271T, 271V, 271W, 271Y, 287A, 287C, 287D, 287E, 287F, 287G, 287H, 287I, 287K, 287M, 287N, 287P, 287Q, 287R, 287S, 287T, 287V, 287W, 287Y, 292A, 292C, 292D, 292E, 292F, 292G, 292H, 292I, 292K, 292M, 292N, 292P, 292Q, 292R, 292S, 292T, 292V, 292W, 292Y, 293A, 293C, 293D, 293E, 293F, 293G, 293H, 293I, 293K, 293L, 293M, 293N, 293P, 293Q, 293S, 293T, 293V, 293W, 293Y, 296A, 296C, 296D, 296E, 296F, 296G, 296H, 296I, 296K, 296L, 296M, 296N, 296P, 296Q, 296S, 296T, 296V, 296W, 296Y, 303A, 303C, 303D, 303E, 303F, 303G, 303H, 303I, 303K, 303L, 303M, 303N, 303Q, 303R, 303S, 303T, 303V, 303W, 303Y, 334A, 334C, 334D, 334E, 334F, 334G, 334H, 334I, 334K, 334L, 334M, 334N, 334P, 334Q, 334R, 334S, 334V, 334W, 334Y, 344A, 344C, 344D, 344E, 344F, 344G, 344I, 344K, 344L, 344M, 344N, 344P, 344Q, 344R, 344S, 344T, 344V, 344W, 344Y, 373A, 373C, 373D, 373E, 373G, 373H, 373I, 373K, 373L, 373M, 373N, 373P, 373Q, 373R, 373S, 373T, 373V, 373W, 373Y, 385A, 385C, 385D, 385E, 385F, 385G, 385H, 385I, 385K, 385L, 385M, 385N, 385Q, 385R, 385S, 385T, 385V, 385W, 및 385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다. 일부 실시양태에서, 폴리뉴클레오티드는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열, 또는 이의 기능성 단편을 포함하는 재조합 리파제를 코딩하고, 재조합 리파제는 M24A, M24C, M24D, M24E, M24F, M24F/G38F/T49V/H70K/E149T/I161L/R174E/L175Q/A189S/L193Q/L225M/E231Q/R243L/V252M/D271G/L287F/L292M/R293Q/R296S/P303A/T334D/H344S/F373Y/P385F, M24G, M24H, M24I, M24K, M24L, M24N, M24P, M24Q, M24R, M24S, M24T, M24V, M24W, M24Y, G38A, G38C, G38D, G38E, G38F, G38H, G38I, G38K, G38L, G38M, G38N, G38P, G38Q, G38R, G38S, G38T, G38V, G38W, G38Y, T49A, T49C, T49D, T49E, T49F, T49G, T49H, T49I, T49K, T49L, T49M, T49N, T49P, T49Q, T49R, T49S, T49V, T49W, T49Y, H70A, H70C, H70D, H70E, H70F, H70G, H70I, H70K, H70L, H70M, H70N, H70P, H70Q, H70R, H70S, H70T, H70V, H70W, H70Y, E149A, E149C, E149D, E149F, E149G, E149H, E149I, E149K, E149L, E149M, E149N, E149P, E149Q, E149R, E149S, E149T, E149V, E149W, E149Y, I161A, I161C, I161D, I161E, I161F, I161G, I161H, I161K, I161L, I161M, I161N, I161P, I161Q, I161R, I161S, I161T, I161V, I161W, I161Y, R174A, R174C, R174D, R174E, R174F, R174G, R174H, R174I, R174K, R174L, R174M, R174N, R174P, R174Q, R174S, R174T, R174V, R174W, R174Y, L175A, L175C, L175D, L175E, L175F, L175G, L175H, L175I, L175K, L175M, L175N, L175P, L175Q, L175R, L175S, L175T, L175V, L175W, L175Y, A189C, A189D, A189E, A189F, A189G, A189H, A189I, A189K, A189L, A189M, A189N, A189P, A189Q, A189R, A189S, A189T, A189V, A189W, A189Y, L193A, L193C, L193D, L193E, L193F, L193G, L193H, L193I, L193K, L193M, L193N, L193P, L193Q, L193R, L193S, L193T, L193V, L193W, L193Y, L225A, L225C, L225D, L225E, L225F, L225G, L225H, L225I, L225K, L225M, L225N, L225P, L225Q, L225R, L225S, L225T, L225V, L225W, L225Y, E231A, E231C, E231D, E231F, E231G, E231H, E231I, E231K, E231L, E231M, E231N, E231P, E231Q, E231R, E231S, E231T, E231V, E231W, E231Y, R243A, R243C, R243D, R243E, R243F, R243G, R243H, R243I, R243K, R243L, R243M, R243N, R243P, R243Q, R243S, R243T, R243V, R243W, R243Y, V252A, V252C, V252D, V252E, V252F, V252G, V252H, V252I, V252K, V252L, V252M, V252N, V252P, V252Q, V252R, V252S, V252T, V252W, V252Y, D271A, D271C, D271E, D271F, D271G, D271H, D271I, D271K, D271L, D271M, D271N, D271P, D271Q, D271R, D271S, D271T, D271V, D271W, D271Y, L287A, L287C, L287D, L287E, L287F, L287G, L287H, L287I, L287K, L287M, L287N, L287P, L287Q, L287R, L287S, L287T, L287V, L287W, L287Y, L292A, L292C, L292D, L292E, L292F, L292G, L292H, L292I, L292K, L292M, L292N, L292P, L292Q, L292R, L292S, L292T, L292V, L292W, L292Y, R293A, R293C, R293D, R293E, R293F, R293G, R293H, R293I, R293K, R293L, R293M, R293N, R293P, R293Q, R293S, R293T, R293V, R293W, R293Y, R296A, R296C, R296D, R296E, R296F, R296G, R296H, R296I, R296K, R296L, R296M, R296N, R296P, R296Q, R296S, R296T, R296V, R296W, R296Y, P303A, P303C, P303D, P303E, P303F, P303G, P303H, P303I, P303K, P303L, P303M, P303N, P303Q, P303R, P303S, P303T, P303V, P303W, P303Y, T334A, T334C, T334D, T334E, T334F, T334G, T334H, T334I, T334K, T334L, T334M, T334N, T334P, T334Q, T334R, T334S, T334V, T334W, T334Y, H344A, H344C, H344D, H344E, H344F, H344G, H344I, H344K, H344L, H344M, H344N, H344P, H344Q, H344R, H344S, H344T, H344V, H344W, H344Y, F373A, F373C, F373D, F373E, F373G, F373H, F373I, F373K, F373L, F373M, F373N, F373P, F373Q, F373R, F373S, F373T, F373V, F373W, F373Y, P385A, P385C, P385D, P385E, P385F, P385G, P385H, P385I, P385K, P385L, P385M, P385N, P385Q, P385R, P385S, P385T, P385V, P385W, 및 P385Y로부터 선택되는 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨진다.
일부 실시양태에서, 상기 기재된 바와 같이, 폴리뉴클레오티드는 본원에 개시된 특성을 갖는 리파제 활성을 갖는 조작된 폴리펩티드를 코딩하고, 폴리펩티드는 참조 서열(예컨대, 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868)에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 동일성을 갖는 아미노산 서열, 또는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에서 개시되는 바와 같은 임의의 변이체의 아미노산 서열, 및 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 참조 폴리펩티드과 비교하여 하나 이상의 잔기 차이, 또는 표에 개시된 바와 같은 임의의 변이체의 아미노산 서열(예컨대, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30개, 또는 그 초ㅘ의 아미노산 잔기 위치)을 포함한다. 일부 실시양태에서, 폴리뉴클레오티드는 본원에 개시된 특성과 함께 리파제 활성을 갖는 조작된 폴리펩티드를 코딩하고, 폴리펩티드는 참조 서열 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 아미노산 서열, 및 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 폴리펩티드와 함께 최적으로 정렬될 때 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 것으로부터 선택되는 잔기 위치에서 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 비교하여 하나 이상의 잔기 차이를 포함한다.
일부 추가의 실시양태에서, 폴리뉴클레오티드는 참조 서열 1, 93, 349, 441, 539, 645, 757, 및/또는 867에 대해 적어도 약 80%, 약 81%, 약 82%, 약 83%, 약 84%, 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 적어도 하나의 폴리뉴클레오티드 서열에 대해 적어도 약 80%, 약 81%, 약 82%, 약 83%, 약 84%, 약 85%, 약 86%, 약 87%, 약 88%, 약 89%, 약 90%, 약 91%, 약 92%, 약 93%, 약 94%, 약 95%, 약 96%, 약 97%, 약 98%, 약 99% 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오티드는 참조 서열 1, 93, 349, 441, 539, 645, 757, 및/또는 867에 대해 적어도 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 적어도 하나의 폴리뉴클레오티드 서열에 대해 적어도 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 실시양태에서, 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드는 서열번호 93, 349, 441, 539, 645, 757, 및/또는 867의 폴리뉴클레오티드 서열을 포함한다.
일부 실시양태에서, 폴리뉴클레오티드는 매우 엄격한 조건 하에서 참조 폴리뉴클레오티드 서열에 혼성화할 수 있다. 일부 실시양태에서, 참조 서열은 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867, 또는 이의 상보체, 또는 본원에 제공된 임의의 변이체 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 서열로부터 선택된다. 일부 실시양태에서, 고도로 엄격한 조건 하에 혼성화할 수 있는 폴리뉴클레오티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제시된 임의의 위치로부터 선택되는 잔기 위치에서 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함하는 리파제 폴리펩티드를 코딩한다. 일부 추가의 실시양태에서, 조작된 폴리뉴클레오티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 것으로부터 선택되거나, 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867로부터 선택되는 참조 서열에 대해 적어도 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 폴리뉴클레오티드를 포함한다. 일부 추가의 실시양태에서, 폴리뉴클레오티드는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 적어도 하나의 폴리뉴클레오티드 서열, 및/또는 및/또는 서열번호 1, 93, 349, 441, 539, 645, 757, 및/또는 867에 대해 적어도 70%, 75%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 서열을 포함한다. 일부 추가의 실시양태에서, 조작된 폴리뉴클레오티드 서열은 참조 폴리뉴클레오티드 서열과 비교하여 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10, 1-15, 1-20, 1-21, 1-22, 1-23, 1-24, 1-25, 1-30, 1-35, 1-40, 1-45, 또는 1-50개 염기 변화를 포함한다. 또 다른 일부 실시양태에서, 조작된 폴리뉴클레오티드 서열은 참조 폴리뉴클레오티드 서열과 비교하여 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 31, 32, 33, 34, 35, 40, 45, 또는 50개 염기 변화를 포함하다. 일부 실시양태에서, 조작된 폴리뉴클레오티드 서열은 참조 폴리뉴클레오티드 서열과 비교하여 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 20, 21, 22, 23, 24, 또는 25개 염기 변화를 포함한다.
일부 실시양태에서, 본원에 제공된 임의의 조작된 리파제 폴리펩티드를 코딩하는 단리된 폴리뉴클레오티드는 유전자의 발현 및 폴리펩티드의 생성을 제공하기 위해 다양한 방식으로 조작된다. 일부 실시양태에서, 폴리펩티드를 코딩하는 폴리뉴클레오티드는 폴리뉴클레오티드 및/또는 폴리펩티드의 발현을 조절하기 위해 하나 이상의 제어 서열이 존재하는 발현 벡터로서 제공된다. 벡터에 삽입하기 전에 단리된 폴리뉴클레오티드를 조작하는 것은 발현 벡터에 따라 바람직하거나 필요할 수 있다. 재조합 DNA 방법을 이용하여 폴리뉴클레오티드 및 핵산 서열을 변형시키는 기술은 당업계에 널리 공지되어 있다.
일부 실시양태에서, 제어 서열은 다른 서열 중에서 프로모터, 리더 서열, 폴리아데닐화 서열, 프로펩티드 서열, 신호 펩티드 서열, 및 전사 터미네이터를 포함한다. 당업계에 공지된 바와 같이, 적합한 제어 서열은 사용되는 숙주 세포에 기초하여 선택될 수 있다. 본 발명은 임의의 특정 제어 서열로 제한되는 것으로 의도되지 않는다.
박테리아 숙주 세포에 대한 예시적인 프로모터는 에쉐리키아 콜라이 락토스 오페론, 트립토판 오페론, 아라비노스 오페론에 대한 유전자로부터 수득되는 프로모터, T7 박테리오파지로부터의 T7 프로모터, 및 사카로폴리스포라 에리트라애(Saccharopolyspora erythraea) 에리트로마이신 내성 유전자를 포함하지만 이에 제한되지 않는다.
사상 진균 숙주 세포에 대한 예시적인 프로모터는 아스페르길루스 오리재(Aspergillus oryzae) TAKA 아밀라제, 리조무코르 미에헤이(Rhizomucor miehei) 아스파르트산 프로테이나제, 아스페르길루스 니게르(Aspergillus niger) 중성 알파-아밀라제, 아스페르길루스 니게르 산 안정성 알파-아밀라제, 아스페르길루스 니게르 또는 아스페르길루스 아와모리(Aspergillus awamori) 글루코아밀라제, 리조무코르 미에헤이 리파제, 아스페르길루스 오리재 알칼리성 프로테아제, 아스페르길루스 오리재 트리오스 포스페이트 이소머라제, 아스페르길루스 니둘란스(Aspergillus nidulans) 아세트아미다제, 및 푸사리움 옥시스포룸(Fusarium oxysporum) 트립신-유사 프로테아제(예컨대 WO 96/00787 참조)에 대한 유전자로부터 수득되는 프로모터 뿐만 아니라 NA2-tpi 프로모터(아스페르길루스 니게르 중성 알파-아밀라제 및 아스페르길루스 오리재 트리오스 포스페이트 이소머라제에 대한 유전자로부터의 프로모터의 하이브리드), 및 이의 돌연변이, 말단절단, 및 하이브리드 프로모터를 포함한다. 예시적인 효모 세포 프로모터는 사카로마이세스 세레비지애 에놀라제(ENO-1), 사카로마이세스 세레비지애 갈락토키나제(GAL1), 사카로마이세스 세레비지애 알콜 데히드로게나제/글리세르알데히드-3-포스페이트 데히드로게나제(ADH2/GAP), 및 사카로마이세스 세레비지애 3-포스포글리세레이트 키나제에 대한 유전자로부터의 것일 수 있다. 효모 숙주 세포에 대한 다른 유용한 프로모터는 당업계에 공지되어 있다(예컨대, 문헌(Romanos et al., Yeast 8:423-488 [1992]) 참조).
포유동물 세포에 사용하기 위한 예시적인 프로모터는 시토메갈로바이러스(CMV), 시미안 액포형성 바이러스 40(SV40)로부터의 것, 호모 사피엔스(Homo sapiens) 포스포글리세레이트 키나제, 베타 액틴, 신장 인자-1a 또는 글리세르알데히드-3-포스페이트 데히드로게나제로부터 것, 갈루스 갈루스(Gallus gallus) β-액틴으로부터의 것을 포함하지만 이에 제한되지 않는다.
일부 실시양태에서, 제어 서열은 전사를 종결시키기 위해 숙주 세포에 의해 인식되는 서열인 적합한 전사 터미네이터 서열이다. 터미네이터 서열은 폴리펩티드를 코딩하는 핵산 서열의 3' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능하는 임의의 터미네이터가 본 발명에서 사용된다. 예컨대, 박테리아 숙주 세포에 대한 예시적인 전사 터미네이터는 T7 터미네이터에 대한 T7 박테리아 파지, 또는 에쉐리키아 콜라이 리보솜 RNA, 예컨대 rrnB 터미네이터로부터 수득될 수 있다. 예컨대, 사상 진균 숙주 세포에 대한 예시적인 전사 터미네이터는 아스페르길루스 오리재 TAKA 아밀라제, 아스페르길루스 니게르 글루코아밀라제, 아스페르길루스 니둘란스 안트라닐레이트 신타제, 아스페르길루스 니게르 알파-글루코시다제, 및 푸사리움 옥시스포룸 트립신-유사 프로테아제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포에 대한 예시적인 터미네이터는 사카로마이세스 세레비지애 에놀라제, 사카로마이세스 세레비지애 시토크롬 C(CYC1), 및 사카로마이세스 세레비지애 글리세르알데히드-3-포스페이트 데히드로게나제에 대한 유전자로부터 수득될 수 있다. 효모 숙주 세포에 대한 다른 유용한 터미네이터는 당업계에 알려져 있다(예컨대, Romanos et al., 상기 참조). 포유동물 세포에 대한 예시적인 터미네이터는 시토메갈로바이러스(CMV), 시미안 액포형성 바이러스 40(SV40)으로부터의 것, 또는 호모 사피엔스 성장 호르몬으로부터의 것을 포함하지만 이에 제한되지 않는다.
일부 실시양태에서, 제어 서열은 숙주 세포에 의한 번역에 중요한 mRNA의 비-번역 영역인 적합한 리더 서열이다. 리더 서열은 폴리펩티드를 코딩하는 핵산 서열의 5' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능적인 임의의 리더 서열이 사용될 수 있다. 사상 진균 숙주 세포에 대한 예시적인 리더는 아스페르길루스 오리재 TAKA 아밀라제 및 아스페르길루스 니둘란스 트리오스 포스페이트 이소머라제에 대한 유전자로부터 수득된다. 효모 숙주 세포에 적합한 리더는 사카로마이세스 세레비지애 에놀라제(ENO-1), 사카로마이세스 세레비지애 3-포스포글리세레이트 키나제, 사카로마이세스 세레비지애 알파-인자, 및 사카로마이세스 세레비지애 알콜 데히드로게나제/글리세르알데히드-3-포스페이트 데히드로게나제(ADH2/GAP)에 대한 유전자로부터 수득되는 것을 포함하지만 이에 제한되지 않는다.
일부 실시양태에서, 제어 서열은 또한 핵산 서열의 3' 말단에 작동 가능하게 연결된 서열인 폴리아데닐화 서열일 수 있고, 이는 전사될 때 숙주 세포에 의해 폴리아데노신 잔기를 전사된 mRNA에 추가하기 위한 신호로서 인식된다. 선택된 숙주 세포에서 기능적인 임의의 폴리아데닐화 서열이 본 발명에서 사용될 수 있다. 사상 진균 숙주 세포에 대한 예시적인 폴리아데닐화 서열은 아스페르길루스 오리재 TAKA 아밀라제, 아스페르길루스 니게르 글루코아밀라제, 아스페르길루스 니둘란스 안트라닐레이트 신타제, 푸사리움 옥시스포룸 트립신-유사 프로테아제, 및 아스페르길루스 니게르 알파-글루코시다제에 대한 유전자로부터의 것을 포함하지만 이에 제한되지 않는다. 효모 숙주 세포에 대한 유용한 폴리아데닐화 서열은 또한 당업계에 공지되어 있다(예컨대, 문헌(Guo and Sherman, Mol. Cell. Biol., 15:5983-5990 [1995]) 참조).
일부 실시양태에서, 제어 서열은 폴리펩티드의 아미노 말단에 연결된 아미노산 서열을 코딩하고 코딩된 폴리펩티드를 세포의 분비 경로로 지시하는 신호 펩티드 코딩 영역이다. 핵산 서열의 코딩 서열의 5' 말단은 본질적으로 번역 판독 프레임에서 분비되는 폴리펩티드를 코딩하는 코딩 영역의 분절과 자연적으로 연결된 신호 펩티드 코딩 영역을 함유할 수 있다. 대안적으로, 코딩 서열의 5' 말단은 코딩 서열에 대해 외래인 신호 펩티드 코딩 영역을 함유할 수 있다.
발현된 폴리펩티드를 선택된 숙주 세포의 분비 경로로 지시하는 임의의 신호 펩티드 코딩 영역이 본원에서 제공되는 조작된 리파제 폴리펩티드의 발현에 사용된다. 사상 진균 숙주 세포에 대한 효과적인 신호 펩티드 코딩 영역은 아스페르길루스 오리재 TAKA 아밀라제, 아스페르길루스 니게르 중성 아밀라제, 아스페르길루스 니게르 글루코아밀라제, 리조무코르 미에헤이 아스파르트산 프로테이나제, 후미콜라 인솔렌스(Humicola insolens) 셀룰라제, 및 후미콜라 라누기노사(Humicola lanuginosa) 리파제에 대한 유전자로부터 수득되는 신호 펩티드 코딩 영역을 포함하지만 이에 제한되지 않는다. 효모 숙주 세포에 유용한 신호 펩티드는 사카로마이세스 세레비지애 알파-인자 및 사카로마이세스 세레비지애 인버타제에 대한 유전자로부터의 것을 포함하지만 이에 제한되지 않는다. 포유동물 숙주 세포에 유용한 신호 펩티드는 면역글로불린 감마(IgG)에 대한 유전자로부터의 것을 포함하지만 이에 제한되지 않는다.
일부 실시양태에서, 제어 서열은 폴리펩티드의 아미노 말단에 위치된 아미노산 서열을 코딩하는 프로펩티드 코딩 영역이다. 생성된 폴리펩티드는 일부 경우에서 "프로엔자임", "프로폴리펩티드" 또는 "자이모겐"으로 지칭된다. 프로폴리펩티드는 프로폴리펩티드로부터 프로펩티드의 촉매적 또는 자가촉매적 절단에 의해 성숙한 활성 폴리펩티드로 전환될 수 있다.
또 다른 측면에서, 본 발명은 또한 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드, 및 도입될 숙주의 유형에 따라 프로모터 및 터미네이터, 복제 기점 등과 같은 하나 이상의 발현 조절 영역을 포함하는 재조합 발현 벡터를 제공한다. 일부 실시양태에서, 상기 기재된 다양한 핵산 및 제어 서열은 함께 결합되어 이러한 부위에서 변이체 리파제 폴리펩티드를 코딩하는 핵산 서열의 삽입 또는 치환을 허용하는 하나 이상의 편리한 제한 부위를 포함하는 재조합 발현 벡터를 생성한다. 대안적으로, 본 발명의 폴리뉴클레오티드 서열(들)은 폴리뉴클레오티드 서열 또는 폴리뉴클레오티드 서열을 포함하는 핵산 구축물을 발현을 위한 적절한 벡터에 삽입함으로써 발현된다. 발현 벡터를 생성함에 있어서, 코딩 서열은 발현을 위한 적절한 제어 서열과 작동 가능하게 연결되도록 코딩 서열이 벡터에 위치된다.
재조합 발현 벡터는 재조합 DNA 절차에 편리하게 적용될 수 있고 변이체 리파제 폴리뉴클레오티드 서열의 발현을 초래할 수 있는 임의의 벡터(예컨대, 플라스미드 또는 바이러스)일 수 있다. 벡터의 선택은 전형적으로 벡터가 도입될 숙주 세포와 벡터의 양립성에 달려 있다. 벡터는 선형 또는 폐쇄형 원형 플라스미드일 수 있다.
일부 실시양태에서, 발현 벡터는 자가 복제 벡터(즉, 염색체외 실체로서 존재하는 벡터로서 이의 복제가 염색체 복제와 무관한 벡터, 예컨대 플라스미드, 염색체외 요소, 미니염색체 또는 인공 염색체)이다. 벡터는 자가 복제를 보장하는 임이의 수단을 포함할 수 있다. 일부 대안적인 실시양태에서, 벡터는 숙주 세포로 도입될 때 게놈에 통합되고 통합된 염색체(들)와 함께 복제되는 것일 수 있다. 또한, 단일 벡터 또는 플라스미드 또는 숙주 세포의 게놈에 도입될 전체 DNA를 함께 함유하는 2개 이상의 벡터 또는 플라스미드, 또는 트랜스포존이 사용될 수 있다.
일부 실시양태에서, 발현 벡터는 바람직하게는 형질전환된 세포의 용이한 선별을 허용하는 하나 이상의 선별 가능한 마커를 함유한다. "선별 가능한 마커"는 살생물제 또는 바이러스 내성, 중금속에 대한 내성, 영양요구성에 대한 원영양 등을 제공하는 생성물의 유전자이다. 박테리아 숙주 세포에 적합한 마커는 카르베니실린, 암피실린, 클로람페니콜, 테트라사이클린, 카나마이신 및 제오신을 포함하지만 이에 제한되지 않는다. 효모 숙주 세포에 적합한 마커는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1, 및 URA3을 포함하지만 이에 제한되지 않는다. 사상 진균 숙주 세포에서 사용하기 위한 선별 가능한 마커는 amdS(아세트아미다제), argB(오르니틴 카르바모일트랜스퍼라제), bar(포스피노트리신 아세틸트랜스퍼라제), hph(하이그로마이신 포스포트랜스퍼라제), niaD(니트레이트 리덕타제), pyrG(오로티딘-5'-포스페이트 데카르복실라제), sC(술페이트 아데닐트랜스퍼라제), 및 trpC(안트라닐레이트 신타제) 뿐만 아니라 이들의 동가물을 포함하지만 이에 제한되지 않는다. 또 다른 측면에서, 본 발명은 본 출원의 적어도 하나의 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드를 포함하는 숙주 세포를 제공하며, 폴리뉴클레오티드는 숙주 세포에서 조작된 리파제 효소(들)의 발현을 위한 하나 이상의 제어 서열에 작동 가능하게 연결된다.
본 발명의 발현 벡터에 의해 코딩되는 폴리펩티드를 발현하는 데 사용하기 위한 숙주 세포는 당업계에 널리 공지되어 있으며, 박테리아 세포(예컨대, 이. 콜라이); 진균 세포, 예컨대 효모 세포(예컨대, 사카로마이세스 세레비지애 및 피키아 파스토리스(Pichia pastoris)[예컨대, ATCC 수탁 번호 201178]); 곤충 세포(예컨대, 드로소필라 S2 및 스포도프테라 Sf9 세포), 식물 세포, 동물 세포(예컨대, CHO, COS, 및 BHK), 및 인간 세포(예컨대, HEK293T, 인간 섬유모세포, THP-1, 저캇 및 바우스(Bowes) 흑색종 세포주)를 포함하지만 이에 제한되지 않는다.
따라서, 또 다른 측면에서, 본 발명은 조작된 리파제 폴리펩티드의 제조 방법을 제공하며, 방법은 폴리펩티드의 발현에 적합한 조건 하에 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드를 발현할 수 있는 숙주 세포를 배양하는 단계를 포함한다. 일부 실시양태에서, 방법은 본원에 기재된 바와 같은 리파제 폴리펩티드를 단리 및/또는 정제하는 단계를 추가로 포함한다.
상기 기재된 숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 당업계에 널리 공지되어 있다. 리파제의 발현을 위한 폴리뉴클레오티드는 당업계에 공지된 다양한 방법에 의해 세포에 도입될 수 있다. 기술은 특히 전기천공법, 유전자총 입자 충격, 리포솜 매개 형질감염, 염화칼슘 형질감염, 및 원형질체 융합을 포함한다.
본원에 개시된 특성을 갖는 조작된 리파제는 천연 발생 또는 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드를 당업계에 공지되고 본원에 기재된 바와 같은 돌연변이유발 및/또는 유도된 진화 방법에 적용함으로써 수득될 수 있다. 예시적인 유도된 진화 기술은 돌연변이유발 및/또는 DNA 셔플링이다(예컨대, 문헌(Stemmer, Proc. Natl. Acad. Sci. USA 91:10747-10751 [1994]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767 및 U.S. Pat. 6,537,746) 참조). 사용될 수 있는 다른 유도된 진화 절차는 특히 시차를 둔 연장 과정(staggered extension process: StEP), 시험관내 재조합(예컨대, 문헌(Zhao et al., Nat. Biotechnol., 16:258-261 [1998]) 참조), 돌연변이유발 PCR(예컨대, 문헌(Caldwell et al., PCR Methods Appl., 3:S136-S140 [1994]) 참조) 및 카세트 돌연변이유발(예컨대, 문헌(Black et al., Proc. Natl. Acad. Sci. USA 93:3525-3529 [1996]) 참조)을 포함한다.
재조합 폴리펩티드는 당업계에 공지된 임의의 적합한 방법을 이용하여 생성될 수 있다. 관심 야생형 폴리펩티드를 코딩하는 유전자는 플라스미드와 같은 벡터에 클로닝될 수 있고, 이. 콜라이 등과 같은 원하는 숙주에서 발현될 수 있다. 재조합 폴리펩티드의 변이체는 당업계에 공지된 다양한 방법에 의해 생성될 수 있다. 실제로, 당업자에게 널리 공지된 다양한 상이한 돌연변이유발 기술이 존재한다. 또한, 돌연변이유발 키트는 많은 상업적 분자 생물학 공급업체로부터 구입할 수 있다. 정의된 아미노산에서 특정 치환(부위 지정), 유전자의 국소 영역에서 특정 또는 무작위 돌연변이(지역 특정), 또는 전체 유전자에 대한 무작위 돌연변이유발(예컨대, 포화 돌연변이유발)을 일으키는 방법을 이용할 수 있다. PCR, 카세트 돌연변이유발, 유전자 합성, 오류가 발생하기 쉬운 PCR, 셔플링, 및 화학적 포화 돌연변이유발, 또는 당업계에 공지된 임의의 다른 적합한 방법을 이용하는 단일 가닥 DNA 또는 이중 가닥 DNA의 부위 지정 돌연변이유발을 포함하지만 이에 제한되지 않는 다양한 적합한 방법이 효소 변이체를 생성하기 위해 당업자에게 공지되어 있다. 돌연변이유발 및 유도된 진화 방법이 발현, 스크리닝 및 검정될 수 있는 변이체 라이브러리를 생성하기 위해 효소 코딩 폴리뉴클레오티드에 쉽게 적용될 수 있다. 임의의 적합한 돌연변이유발 및 유도된 진화 방법이 본 발명에서 이용되고, 당업계에 널리 공지되어 있다(예컨대, 미국 특허 번호 5,605,793, 5,811,238, 5,830,721, 5,834,252, 5,837,458, 5,928,905, 6,096,548, 6,117,679, 6,132,970, 6,165,793, 6,180,406, 6,251,674, 6,265,201, 6,277,638, 6,287,861, 6,287,862, 6,291,242, 6,297,053, 6,303,344, 6,309,883, 6,319,713, 6,319,714, 6,323,030, 6,326,204, 6,335,160, 6,335,198, 6,344,356, 6,352,859, 6,355,484, 6,358,740, 6,358,742, 6,365,377, 6,365,408, 6,368,861, 6,372,497, 6,337,186, 6,376,246, 6,379,964, 6,387,702, 6,391,552, 6,391,640, 6,395,547, 6,406,855, 6,406,910, 6,413,745, 6,413,774, 6,420,175, 6,423,542, 6,426,224, 6,436,675, 6,444,468, 6,455,253, 6,479,652, 6,482,647, 6,483,011, 6,484,105, 6,489,146, 6,500,617, 6,500,639, 6,506,602, 6,506,603, 6,518,065, 6,519,065, 6,521,453, 6,528,311, 6,537,746, 6,573,098, 6,576,467, 6,579,678, 6,586,182, 6,602,986, 6,605,430, 6,613,514, 6,653,072, 6,686,515, 6,703,240, 6,716,631, 6,825,001, 6,902,922, 6,917,882, 6,946,296, 6,961,664, 6,995,017, 7,024,312, 7,058,515, 7,105,297, 7,148,054, 7,220,566, 7,288,375, 7,384,387, 7,421,347, 7,430,477, 7,462,469, 7,534,564, 7,620,500, 7,620,502, 7,629,170, 7,702,464, 7,747,391, 7,747,393, 7,751,986, 7,776,598, 7,783,428, 7,795,030, 7,853,410, 7,868,138, 7,783,428, 7,873,477, 7,873,499, 7,904,249, 7,957,912, 7,981,614, 8,014,961, 8,029,988, 8,048,674, 8,058,001, 8,076,138, 8,108,150, 8,170,806, 8,224,580, 8,377,681, 8,383,346, 8,457,903, 8,504,498, 8,589,085, 8,762,066, 8,768,871, 8,849,575, 9,593,326, 9,665,694, 9,684,771, 9,803,224, 9,864,833, 9.821,613, 9,996,661, 10,738,286, 및 모든 관련된 US 뿐만 아니라 PCT 및 비-US 대응물; Ling et al., Anal. Biochem., 254(2):157-78 [1997]; Dale et al., Meth. Mol. Biol., 57:369-74 [1996]; Smith, Ann. Rev. Genet., 19:423-462 [1985]; Botstein et al., Science, 229:1193-1201 [1985]; Carter, Biochem. J., 237:1-7 [1986]; Kramer et al., Cell, 38:879-887 [1984]; Wells et al., Gene, 34:315-323 [1985]; Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]; Christians et al., Nat. Biotechnol., 17:259-264 [1999]; Crameri et al., Nature, 391:288-291 [1998]; Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]; Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]; Crameri et al., Nat. Biotechnol., 14:315-319 [1996]; Stemmer, Nature, 370:389-391 [1994]; Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767; 및 WO 2009/152336, 모두 참조로 본원에 포함됨).
일부 실시양태에서, 돌연변이유발 처리 후에 수득되는 효소 변이체는 효소 변이체를 정의된 온도 (또는 다른 검정 조건)에 적용하고 열처리 또는 다른 검정 조건 후에 남아 있는 효소 활성의 양을 측정함으로써 스크리닝된다. 이어서, 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드를 함유하는 DNA는 숙주 세포로부터 단리되고, (존재하는 경우) 뉴클레오티드 서열 변화를 확인하기 위해 서열분석되고, 상이하거나 동일한 숙주 세포에서 효소를 발현하는 데 사용된다. 발현 라이브러리로부터 효소 활성을 측정하는 것은 당업계에 공지된 임의의 적합한 방법(예컨대, HPLC 분석과 같은 표준 생화학 기술)을 이용하여 수행될 수 있다.
공지된 서열의 조작된 폴리펩티드의 경우, 효소를 코딩하는 폴리뉴클레오티드는 공지된 합성 방법에 따라 표준 고체상 방법에 의해 제조될 수 있다. 일부 실시양태에서, 최대 약 100개의 염기의 단편을 개별적으로 합성한 다음, (예컨대, 효소적 또는 화학적 리게이션 방법, 또는 폴리머라제 매개 방법에 의해) 결합하여 임의의 원하는 연속 서열을 형성할 수 있다. 예컨대, 본원에 개시된 폴리뉴클레오티드 및 올리고뉴클레오티드는 고전적인 포스포르아미다이트 방법을 이용하는 화학적 합성에 의해 제조될 수 있으며(예컨대, 문헌(Beaucage et al., Tetra. Lett., 22:1859-69 [1981]; 및 Matthes et al., EMBO J., 3:801-05 [1984]) 참조), 이는 전형적으로 자동화된 합성 방법에서 실시되기 때문이다. 포스포르아미다이트 방법에 따라 (예컨대, 자동 DNA 합성기에서) 올리고뉴클레오티드가 합성되고, 정제되고, 어닐링되고, 리게이션되고, 적절한 벡터에 클로닝된다. 그러나, 임의의 적합한 방법이 본 발명에서 이용되므로, 본 발명은 폴리뉴클레오티드 및 올리고뉴클레오티드의 생성을 위한 임의의 특정 방법으로 제한되는 것으로 의도되지 않는다.
따라서, 일부 실시양태에서, 조작된 리파제 폴리펩티드를 제조하는 방법은 (a) 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1 뿐만 아니라 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868에 제공된 임의의 변이체의 아미노산 서열로부터 선택되는 아미노산 서열을 포함하는 폴리펩티드를 코딩하는 폴리뉴클레오티드를 합성하는 단계, 및 (b) 폴리뉴클레오티드에 의해 코딩되는 리파제 폴리펩티드를 발현하는 단계를 포함할 수 있다. 방법의 일부 실시양태에서, 폴리뉴클레오티드에 의해 코딩되는 아미노산 서열은 하나 또는 여러 개(예컨대, 최대 3, 4, 5, 또는 최대 10개) 아미노산 잔기 결실, 삽입 및/또는 치환을 포함한다. 일부 실시양태에서, 아미노산 서열은 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10, 1-15, 1-20, 1-21, 1-22, 1-23, 1-24, 1-25, 1-30, 1-35, 1-40, 1-45, 또는 1-50개의 아미노산 잔기 결실, 삽입 및/또는 치환을 포함한다. 일부 실시양태에서, 아미노산 서열은 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 또는 50개의 아미노산 잔기 결실, 삽입 및/또는 치환을 포함한다. 일부 실시양태에서, 아미노산 서열은 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 20, 21, 22, 23, 24, 또는 25개의 아미노산 잔기 결실, 삽입 및/또는 치환을 포함한다. 일부 실시양태에서, 치환은 보존적 및/또는 비-보존적 치환을 포함한다.
발현된 조작된 리파제 폴리펩티드는 본원에 기재된 검정 및 조건을 포함하지만 이에 제한되지 않는 당업계에 공지된 임의의 적합한 검정을 이용하여 임의의 원하는 개선된 특성(예컨대, 활성, 선택성, 안정성, 내산성, 프로테아제 민감성 등)에 대해 평가될 수 있다.
일부 실시양태에서, 숙주 세포에서 발현되는 임의의 조작된 리파제 폴리펩티드는 특히 리소자임 처리, 음파 처리, 여과, 염석, 열처리, 초원심분리 및 크로마토 그래피를 포함하는 단백질 정제를 위한 널리 공지된 기술 중 임의의 하나 이상을 이용하여 세포 및/또는 배양 배지로부터 회수된다.
리파제 폴리펩티드의 단리를 위한 크로마토그래피 기술은 특히 역상 크로마토그래피 고성능 액체 크로마토그래피, 이온 교환 크로마토그래피, 소수성 상호작용 크로마토그래피, 겔 전기영동, 및 친화성 크로마토그래피를 포함한다. 특정 효소를 정제하기 위한 조건은 부분적으로 순전하, 소수성, 친수성, 분자량, 분자 모양 등과 같은 인자에 의존하며, 이는 당업자에게 자명할 것이다. 일부 실시양태에서, 친화성 기술을 이용하여 개선된 변이체 리파제 효소를 단리할 수 있다. 친화성 크로마토그래피 정제를 이용하는 일부 실시양태에서, 변이체 리파제 폴리펩티드에 특이적으로 결합하는 임의의 항체가 사용된다.
친화성 크로마토그래피 정제를 이용하는 일부 실시양태에서, 리파제에 공유적으로 부착된 글리칸에 결합하는 단백질이 사용된다. 친화성 크로마토그래피 정제를 이용하는 또 다른 실시양태에서, 리파제 활성 부위에 결합하는 임의의 소분자가 사용된다. 항체 생성을 위해, 토끼, 마우스, 래트 등을 포함하지만 이에 제한되지 않는 다양한 숙주 동물에 폴리펩티드(예컨대, 리파제 변이체) 또는 이의 단편을 주사하여 면역화시킨다. 일부 실시양태에, 리파제 폴리펩티드 또는 단편은 측쇄 작용기 또는 측쇄 작용기에 부착된 링커에 의해 BSA와 같은 적합한 캐리어에 부착된다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 조작된 리파제 폴리펩티드의 생성에 도움이 되는 조건 하에 본원에 기재된 바와 같은 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 서열을 포함하는 숙주 세포(예컨대, 이. 콜라이, 에스. 세레비지애, 다우쿠스 카로타(Daucus carota), 니코티아나 타바쿰(Nicotiana tabacum), 에이치. 사피엔스(H. sapiens)(예컨대, HEK293T), 또는 크리세툴루스 그리세우스(Cricetulus griseus)(예컨대, CHO))를 배양하는 단계 및 세포 및/또는 배양 배지로부터 조작된 리파제 폴리펩티드를 회수하는 단계를 포함하는 방법에 의해 숙주 세포에서 생성된다.
일부 실시양태에서, 본 발명은 조작된 리파제 폴리펩티드의 생성을 허용하는 적합한 배양 조건 하에 참조 서열(예컨대, 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868)에 대해 적어도 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 95%, 96%, 97%, 98%, 99%, 또는 100% 서열 동일성, 및 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 아미노산 서열과 최적으로 정렬 시, 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에 제공된 것으로부터 선택되는, 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868과 비교하여 하나 이상의 아미노산 잔기 차이, 및/또는 이들의 조합을 갖는, 조작된 리파제 폴리펩티드를 코딩하는 폴리뉴클레오티드 서열을 포함하는 재조합 진핵 세포를 배양하는 단계 및 임의적으로 배양물 및/또는 배양된 박테리아 세포로부터 조작된 리파제 폴리펩티드를 회수하는 단계를 포함하는, 조작된 리파제 폴리펩티드의 제조 방법을 포괄한다.
일부 실시양태에서, 조작된 폴리펩티드가 재조합 숙주 세포 또는 세포 배양 배지로부터 회수되면, 이들은 당업계에 공지된 임의의 적합한 방법(들)에 의해 추가로 정제된다. 일부 추가의 실시양태에서, 정제된 리파제 폴리펩티드는 다른 성분 및 화합물과 조합되어 상이한 적용 및 용도(예컨대, 약학적 조성물)에 적합한 조작된 리파제 폴리펩티드를 포함하는 조성물 및 제형을 제공한다. 일부 추가의 실시양태에서, 정제된 v 폴리펩티드, 또는 제형화된 리파제 폴리펩티드는 동결건조된다.
조성물:
본 발명은 하기 기재되는 것들을 포함하지만 이에 제한되지 않는 다양한 조성물 및 포맷을 제공한다. 일부 실시양태에서, 본 발명은 식이 및/또는 영양 보충제와 같은 약학적 및 다른 조성물에 사용하기에 적합한 조작된 리파제 폴리펩티드를 제공한다.
투여 방식에 따라, 치료학적 유효량의 본 발명에 따른 조작된 리파제를 포함하는 이러한 조성물은 고체, 반고체 또는 액체 형태이다. 일부 실시양태에서, 조성물은 희석제, 버퍼, 부형제, 염, 유화제, 보존제, 안정화제, 충전제 및 다른 성분과 같은 다른 약학적으로 허용되는 성분을 포함한다. 제형화 및 투여를 위한 기술에 대한 세부사항은 당업계에 널리 공지되어 있고 문헌에 기재되어 있다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 약학적 조성물에 사용하기 위해 제형화된다. 환약, 정제, 겔 탭, 캡슐, 로젠지, 당의정, 분말, 연질 겔, 졸-겔, 겔, 에멀젼, 임플란트, 패치, 스프레이, 연고, 도찰제, 크림, 페이스트, 젤리, 페인트, 에어로졸, 츄잉껌, 완화제, 스틱, 용액, 현탁액(유성 현탁액, 수중유 에멀젼 등을 포함하지만 이에 제한되지 않음), 슬러리, 시럽, 제어 방출 제형, 좌제 등을 포함하지만 이에 제한되지 않는 조작된 리파제 폴리펩티드를 전달하는 데 사용하기 위한 임의의 적합한 포맷이 본 발명에서 사용된다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 주사 또는 주입에 적합한 포맷(즉, 주사 제형)으로 제공된다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 실리카 기반(예컨대, 옥시실란) 졸-겔을 포함하는 졸-겔과 같은 생체적합성 매트릭스에 제공된다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 캡슐화되고/되거나 장용으로 코팅된다. 일부 대안적인 실시양태에서, 조작된 리파제 폴리펩티드는 나노구조(예컨대, 나노튜브, 나노소관, 나노캡슐, 또는 마이크로캡슐, 미소구체, 리포솜 등)에 캡슐화된다. 실제로, 본 발명은 임의의 특정 전달 제형 및/또는 전달 수단으로 제한되는 것으로 의도되지 않는다. 조작된 리파제 폴리펩티드는 비경구, 경구, 국소, 경피, 비강내, 안내, 경막내, 임플란트 등을 포함하지만 이에 제한되지 않는 당업계에 공지된 임의의 적합한 수단에 의해 투여되는 것으로 의도된다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 글리코실화, 화학적 가교 시약, 페길화(즉, 폴리에틸렌 글리콜[PEG] 또는 활성화된 PEG 등으로 변형됨) 또는 다른 화합물에 의해 화학적으로 변형된다(예컨대, 문헌(Ikeda, Amino Acids 29:283-287 [2005]; 미국 특허 번호 7,531,341, 7,534,595, 7,560,263, 및 7,53,653; 미국 특허 출원 공개 번호 2013/0039898, 2012/0177722 등) 참조). 실제로, 본 발명은 임의의 특정 전달 방법 및/또는 메커니즘으로 제한되는 것으로 의도되지 않는다.
일부 추가의 실시양태에서, 조작된 리파제 폴리펩티드는 매트릭스 안정화된 효소 결정을 포함하는 제형으로 제공된다. 일부 실시양태에서, 제형은 가교된 결정질의 조작된 리파제 효소 및 효소 결정에 부착되는 반응성 모이어티를 갖는 중합체를 포함한다. 본 발명은 또한 중합체에 조작된 리파제 폴리펩티드를 제공한다.
일부 실시양태에서, 본 발명의 조작된 리파제 폴리펩티드를 포함하는 조성물은 슈가(예컨대, 락토스, 수크로스, 만니톨 및/또는 소르비톨), 전분(예컨대, , 옥수수, 밀, 쌀, 감자 또는 다른 식물 전분), 셀룰로스(예컨대, 메틸 셀룰로스, 히드록시프로필메틸 셀룰로스, 나트륨 카르복시-메틸셀룰로스), 검(예컨대, 아라비아, 트라가칸트, 구아 등) 및/또는 단백질(예컨대, 젤라틴, 콜라겐 등)을 포함하지만 이에 제한되지 않는 하나 이상의 일반적으로 사용되는 담체 화합물을 포함한다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 식이 지방 흡수를 개선하고 대변에서 식이 지질을 감소시키는데 사용하기에 적합하다. 일부 실시양태에서, 본 발명은 혈액, 뇌척수액 등과 같은 유체에서 당지질의 농도를 감소시키는 데 사용하기에 적합한 조작된 리파제 폴리펩티드를 제공한다. 투여되는 조작된 리파제 폴리펩티드(들)의 용량은 병태 또는 질환, 대상체의 일반적 상태, 및 당업자에게 알려진 다른 요인에 의존한다. 일부 실시양태에서, 조성물은 단일 또는 다중 투여를 위한 것이다. 일부 실시양태에서, 췌장 기능부전 질환을 갖는 인간에게 투여되는 조성물(들) 중 조작된 리파제 폴리펩티드(들)의 농도는 질환(예컨대, 췌장 기능부전 질환)을 효과적으로 치료 및/또는 개선하기에 충분한 것으로 고려된다. 일부 실시양태에서, 조작된 리파제 폴리펩티드는 췌장 효소 기능부전과 같은 질환의 치료를 위해 아밀라제 및/또는 프로테아제와 같은 다른 효소와 조합하여 사용된다.
일부 실시양태에서, 조작된 리파제 폴리펩티드는 식이 보충제, 약효식품 등을 포함하지만 이에 제한되지 않는 다른 약학적 및/또는 식이 조성물과 조합하여 투여된다. 임의의 적합한 방법 및/또는 형태가 사용되므로, 본 발명은 임의의 특정한 투여 방법 또는 형태로 제한되는 것으로 의도되지 않는다.
실험
달성된 실험 및 결과를 포함하는 하기 실시예는 예시 목적으로만 제공되며 본 발명을 제한하는 것으로 해석되어서는 안 된다.
하기 실험적 개시에서, 하기 약어가 적용된다: ppm(백만분의 일); M(몰); mM(밀리몰), uM 및 μM(마이크로몰); nM(나노몰); mol(몰); gm 및 g(그램); mg(밀리그램); ug 및 μg(마이크로그램); L 및 l(리터); ml 및 mL(밀리리터); cm(센티미터); mm(밀리미터); um 및 μm(마이크로미터); sec.(초); min(분); h 및 hr(시); U(단위); MW(분자량); rpm(분당 회전수); ℃(섭씨 온도); CDS(코딩 서열); DNA(데옥시리보핵산); RNA(리보핵산); 이. 콜라이 W3110(일반적으로 사용되는 실험실 이. 콜라이 균주, Coli Genetic Stock Center[CGSC](New Haven, CT)에서 입수 가능); EPI(외분비 췌장 기능부전); LIP 및 lip(리파제); btLIP(비. 써모아밀로보란스(B. thermoamylovorans) 리파제); HPLC(고압 액체 크로마토그래피); ms(질량 분광측정법 또는 질량 분광광도법); SDS-PAGE(나트륨 도데실 술페이트 폴리아크릴아미드 겔 전기영동); PES(폴리에테르술폰); ACN(아세토니트릴); IPA(이소프로필 알콜); IPTG(이소프로필 β-D-1-티오갈락토피라노사이드); PMBS(폴리믹신 B 술페이트); NADPH(니코틴아미드 아데닌 디뉴클레오티드 포스페이트); LB(루리아 브로쓰); PBS(포스페이트 완충 식염수); MeOH(메탄올); TAG(트리올레인); DAG(디올레인); MAG(모놀레인); OA(올레산); FIOPC 및 FIOP(양성 대조군에 비해 개선된 배수); HTP(고처리량); CAV(세포 가속기 전압; 충돌 세포 가속기 전압); CE(충돌 에너지); RF(무선 주파수); 싱클레어(Sinclair Research, 및 Sinclair Bio Resources, Auxvasse, MO); 시그마-알드리치(Sigma-Aldrich, St. Louis, MO); 폴 코프.(Pall, Corp., Pt. Washington, NY); 밀리포어(Millipore, Corp., Billerica MA); 디프코(Difco Laboratories, BD Diagnostic Systems, Detroit, MI); 몰리큘라 디바이시스(Molecular Devices, LLC, Sunnyvale, CA); 쿠너(Adolf Kuhner, AG, Basel, Switzerland); 어플라이드 바이오시스템스(Applied Biosystems, Life Technologies, Corp.의 일부, Grand Island, NY), 아질런트(Agilent Technologies, Inc., Santa Clara, CA); RAPIDFIRE® MS(RAPIDFIRE® 질량 분광계, Agilent); 써모 사이언티픽(ThermoFisher Scientific의 일부, Waltham, MA); 집코(ThermoFisher Scientific); 피어스(Pierce Biotechnology(현재 Thermo Fisher Scientific의 일부), Rockford, IL); 써모피셔 사이언티픽(Thermo Fisher Scientific, Waltham, MA); 코닝(Corning, Inc., Palo Alto, CA); 애브비(AbbVie, Inc., North Chicago, IL); 및 바이오-라드(Bio-Rad Laboratories, Hercules, CA).
실시예 1
박테리아 리파제 유전자 획득 및 발현 벡터의 구축
바실루스 써모아밀로보란스(Bacillus thermoamylovorans) 리파제(서열번호: 2)를 코딩하는 DNA 서열은 이. 콜라이에서의 발현을 위해 코돈 최적화되었고, 이. 콜라이 발현 벡터 pCK110900 벡터 시스템(예컨대, 미국 특허 번호 7,629,157, 및 9,714,437, 및 미국 특허 출원 공개 2006/0195947(이들 모두는 본원에 참조로 포함됨) 참조) 또는 pJV110900 벡터 시스템(예컨대, 미국 특허 출원 공개 2017/213758(본원에 참조로 포함됨) 참조)으로 클로닝되었다. 그러나, 본 발명은 임의의 특정 벡터로 제한되는 것으로 의도되지 않는다. 또한, 일부 실시양태에서, 항미생물 내성 마커가 결여된 발현 벡터가 사용된다. 플라스미드 구축물은 W3110에서 유래된 이. 콜라이 균주로 형질전환되었다. 당업자에게 일반적으로 공지된 유도된 진화 기술을 이용하여 이 플라스미드 구축물(예컨대, 미국 특허 번호 8,383,346 및 WO2010/144103 참조) 뿐만 아니라 이의 유도체로부터 유전자 변이체의 라이브러리를 생성하였다.
실시예 2
포유동물 리파제 유전자 획득, 발현 벡터의 구축 및 HTP 성장 및 활성 스크린
표 1의 포유동물 리파제를 코딩하는 유전자(서열번호 1, 3, 5, 7, 9, 및 11)는 사카로마이세스 세레비지애에서의 발현을 위해 코돈 최적화되었다. 에스. 세레비지애 교배 인자 알파(MFα) 신호 펩티드는 리파제의 분비된 발현을 위해 성숙한 형태의 리파제에 유전자 조작으로 융합되었다. 이들 효모 균주를 WO 2016/105889(본원에 참고로 포함됨)에 기재된 바와 같이 HTP로 성장시켰다. 이들 효모 배양물로부터의 상청액을 실시예 3에 기재된 바와 같이 활성에 대해 검정하였다. 이들 리파제로부터 수득된 활성이 표 2-1에 나타나 있다.
[표 2-1]
실시예 3
리파제 변이체의 고처리량(HTP) 성장 및 스크리닝 조건
리파제 변이체의 성장 및 스크리닝에 대해 수행된 실험이 하기에 기재되어 있다.
비. 써모아밀로보란스 리파제(btLIP) 및 리파제(LIP) 변이체의 고처리량(HTP) 성장:
형질전환된 이. 콜라이 세포를 선별물과 함께 1% 글루코스를 함유하는 LB 한천 플레이트 상에 플레이팅함으로써 선별하였다. 37℃에서 밤새 인큐베이션한 후, 콜로니를 1% 글루코스 및 선별물로 보충된 180 μl/웰 LB로 충전된 96 웰 얕은 평저 플레이트(NUNCTM, Thermo-Scientific)의 웰에 배치하였다. 배양물을 쉐이커 인큐베이터(200 rpm, 30℃ 및 85% 상대 습도; Kuhner)에서 18-20시간 동안 밤새 성장시켰다. 밤새 성장된 샘플(20 μL)을 선별 화합물(예컨대, 클로람페니콜)로 보충된 380 μL의 테리픽 브로쓰(Terrific Broth)로 충전된 COSTAR® 96 웰 딥 플레이트로 옮겼다. 플레이트를 쉐이커 인큐베이터(250 rpm, 30℃, 85% 상대 습도; Kuhner)에서 135분 동안 인큐베이션하였다. 이어서, 리파제 변이체의 발현을 멸균수 중의 40 μL의 10 mM IPTG로 유도하고, 배양물을 쉐이커 인큐베이터(250 rpm, 30℃ 및 85% 상대 습도; Kuhner)에서 20-24시간 동안 밤새 인큐베이션하였다. 세포를 원심분리(4000 rpm x 20분)에 의해 펠렛화하고, 상층액을 버리고, 세포를 분석 전에 -80℃에서 동결시켰다.
HTP 펠렛의 용해:
먼저, 200-400 μL의 용해 버퍼(1X PBS, 1 mg/ml 리소자임, 및 0.5 mg/ml 폴리믹신 B 술페이트)을 세포 펠렛에 첨가하였다. 세포 펠렛 및 버퍼를 실온에서 1.5-2시간 동안 부드럽게 진탕하고, 본원에 기재된 다양한 HTP 검정에 정화된 용해물을 사용하기 전에 원심분리(4000 rpm x 5분)하였다. SDS-PAGE에 의한 이러한 용해물의 분석은 btLIP의 예상 MW와 일치하는 ~45 kDa의 겉보기 MW에서 과발현된 단백질의 존재를 밝혀내었다.
리파제 활성에 대한 정화된 용해물의 분석:
btLIP 변이체 활성은 올레산, 디올레인 및 모노올레인의 형성을 시간 경과에 따른 풍부도의 변화에 의해 측정함으로써 결정되었다. 이 검정을 위해, 90 μL의 100 mM 인산나트륨 및 2.5 μL 트리올레인 pH 7.0을 10 μL의 용해물과 혼합하고, 폴리아크릴레이트 96 웰 미세역가 플레이트(COSTAR® 플레이트 #3635, Corning)의 웰에 첨가하고, 37℃에서 30분 내지 1시간 동안 인큐베이션하였다. 반응물을 600 μL ACN:IPA(1:3)로 켄칭시키고, 원심분리로 정화하고, 214 nm에서 올레산, 디올레인, 모노올레인 및 트리올레인을 검출하기 위해 아세토니트릴 중 55% IPA의 등용매 유동으로 실행된 C18 POROSHELL® 5 μm 150 mm 컬럼에서 분리하였다. 대안적으로, 켄칭된 반응물을 정화하고, 10 μL을 190 μL 50:50 IPA:MeOH로 희석하고, 1분 동안 진탕한 다음, 10 μL을 190 μL의 50:25:25 H2O:IPA:MeOH에 첨가하여 다시 한번 희석하였다. 이어서, 샘플을 RAPIDFIRE® MS로 분석하였다. 하기 실시예에서, "도전받지 않은 활성"은 하기 검정 방법 설명에 기재된 바와 같이 임의의 외부 전처리/도전 없이 수행된 리파제 활성 측정을 지칭한다.
[표 3-1]
열 전처리로 전처리된 정화된 용해물의 HTP 분석:
btLIP 변이체의 활성은 37℃ 내지 65℃에서 인큐베이션 후에 결정되었다. 먼저, 100 μL의 정화된 용해물을 96 웰 바이오라드 하드-쉘(Hard-Shell) PCR 얇은 벽 미세역가 플레이트(#hsp9601; BioRad)의 웰에 첨가하였다. 플레이트를 밀봉하고, 분석 전에 열순환기에서 1시간 동안 37℃ 내지 65℃에서 인큐베이션하였다. 변이체 활성은 올레산, 디올레인 및 모노올레인의 형성을 시간 경과에 따른 풍부도의 변화로 측정하여 결정되었다. 이 검정을 위해, 90 μL의 100 mM 인산나트륨 및 2.5 μL 트리올레인 pH 7.0을 10 μL의 용해물과 혼합하고, 폴리아크릴레이트 96 웰 미세역가 플레이트(COSTAR® 플레이트 #3635; Corning)의 웰에 첨가하고, 37℃에서 30분 내지 1시간 동안 인큐베이션하였다. 반응물을 600 μL ACN:IPA(1:3)로 켄칭시키고, 정화하고, 10 μL를 190 μL 50:50 IPA:MeOH로 희석하고, 1분 동안 진탕한 다음, 10 μL를 의 190 μL의 50:25:25 H2O:IPA:MeOH에 첨가함으로써 다시 한번 희석하였다. 이어서, 샘플을 RAPIDFIRE MS로 분석하였다. 결과는 실시예 4의 표에 제공된다.
pH가 낮고 펩신 전처리가 있거나 없는 정화된 용해물의 HTP 분석:
btLIP 변이체의 활성은 위 환경을 가장하기 위해 펩신과 함께 인큐베이션 후에 결정되었다. 먼저, 90 μL의 100 mM 나트륨 시트레이트, 1.5 mg/mL 펩신(P7000 Sigma)이 있거나 없는 2.5 mg TAG pH 2.0 내지 5.0, 및 10 μL의 정화된 용해물을 96 웰 환저 미세역가 플레이트(COSTAR® 플레이트 #3798; Corning)의 웰에 첨가하였다. 플레이트를 밀봉하고, 분석 전에 1시간 동안 진탕하면서 37℃에서 인큐베이션하였다(THERMOTRON® 쉐이커 HT Infors AJ185, 400 rpm, 1" 스로(throw)). 변이체 활성은 올레산, 디올레인 및 모노올레인의 형성을 시간 경과에 따른 풍부도의 변화로 측정하여 결정되었다. 이 검정을 위해, 90 μL의 100 mM 인산나트륨 및 2.5 μL 트리올레인 pH 7.0을 10 μL의 펩신 반응 혼합물과 혼합하고, 폴리아크릴레이트 96 웰 미세역가 플레이트(COSTAR® 플레이트 #3635; Corning)의 웰에 첨가하고, 37℃에서 30분 내지 1시간 동안 인큐베이션하였다. 반응물을 600 μL ACN:IPA(1:3)로 켄칭시키고, 정화하고, 10 μL를 190 μL 50:50 IPA:MeOH로 희석하고, 1분 동안 진탕한 다음, 10 μL를 190 μL의 50:25:25 H2O:IPA:MeOH에 첨가하여 다시 한번 희석하였다. 이어서, 샘플을 RAPIDFIRE® MS로 분석하였다. 결과는 실시예 4의 표에 제공된다.
프로테아제 전처리를 갖는 정화된 용해물의 HTP 분석:
btLIP 변이체의 활성은 하부 장의 환경을 가장하기 위해 키모트립신 및 트립신과의 인큐베이션 후에 결정되었다. 먼저, 50 μL의 프로테아제 믹스(0.01 - 100 mg/ml 키모트립신(C4129, Sigma), 0.01 - 100 mg/ml 트립신(T7409, Sigma)), 100 mM 인산나트륨 pH 6.5 to 7.0 중의 0 - 30 μL의 20 mM 소듐 타우로콜레이트, 및 50 μL의 정화된 용해물을 96 웰 환저 미세역가 플레이트(COSTAR® 플레이트 #3798; Corning)의 웰에 첨가하였다. 플레이트를 밀봉하고, 분석 전에 1시간 동안 진탕하면서 37℃에서 인큐베이션하였다(THERMOTRON® 쉐이커 HT Infors AJ185, 400 rpm, 1" 스로). 변이체 활성은 올레산, 디올레인 및 모노올레인의 형성을 시간 경과에 따른 풍부도의 변화로 측정하여 결정되었다. 이 검정을 위해, 90 μL의 100 mM 인산나트륨 및 2.5 μL 트리올레인 pH 7.0을 10 μL의 프로테아제 반응 혼합물과 혼합하고, 폴리아크릴레이트 96 웰 미세역가 플레이트(COSTAR® 플레이트 #3635; Corning)의 웰에 첨가하고, 37℃에서 30분 내지 1시간 동안 인큐베이션하였다. 반응물을 600 μL ACN:IPA(1:3)로 켄칭시키고, 정화하고, 10 μL을 190 μL 50:50 IPA:MeOH로 희석하고, 1분 동안 진탕한 다음, 10 μL을 190 μL의 50:25:25 H2O:IPA:MeOH에 첨가하여 다시 한번 희석하였다. 이어서, 샘플을 RAPIDFIRE® MS로 분석하였다. 결과는 실시예 4의 표에 제공된다.
다단계 위장(GI) 도전을 갖는 정화된 용해물의 HTP 분석:
btLIP 변이체의 활성은 37℃ 내지 65℃에서 인큐베이션한 후에 결정되었다. 먼저, 100 μL의 정화된 용해물을 96 웰 바이오라드 하드-쉘 PCR 얇은 벽 미세역가 플레이트(#hsp9601; BioRad)의 웰에 첨가하였다. 플레이트를 밀봉하고, 위 도전 전에 1시간 동안 열순환기에서 37℃ 내지 65℃에서 인큐베이션하였다. 이어서, btLIP 변이체를 펩신과 함께 인큐베이션하여 위 환경을 가장하였다. 먼저, 3.0 mg/mL 펩신(P7000 Sigma)과 함께 50 μL의 100 mM 나트륨 시트레이트 pH 2.0 내지 5.0, 및 50 μL의 열처리된 용해물을 96 웰 환저 미세역가 플레이트(COSTAR® 플레이트 #3798; Corning)의 웰에 첨가하였다. 플레이트를 밀봉하고, 분석 전 1시간 동안 진탕하면서 37℃에서 인큐베이션하였다(THERMOTRON® 쉐이커 HT Infors AJ185, 400 rpm, 1" 스로). 이어서, btLIP 변이체를 키모트립신 및 트립신과 함께 인큐베이션하여 하부 장 환경을 가장하였다. 이 단계에서, 100 μL의 프로테아제 믹스(0.01 - 100 mg/ml 키모트립신[C4129; Sigma] 및 0.01 -100 mg/ml 트립신[T7409; Sigma]), 100 mM 인산나트륨 pH 6.5 내지 7.0 중의 0 - 30 μL의 20 mM 나트륨 타우로콜레이트, 및 100 μL의 열처리된 위 도전 용해물을 96 웰 환저 미세적정 플레이트(COSTAR® 플레이트 #3798; Corning)의 웰에 첨가하였다. 플레이트를 밀봉하고, 분석 전에 1시간 동안 진탕하면서 37℃에서 인큐베이션하였다(THERMOTRON® 쉐이커 HT Infors AJ185, 400 rpm, 1" 스로). 변이체 활성은 올레산, 디올레인 및 모노올레인의 형성을 시간 경과에 따른 풍부도의 변화로 측정하여 결정되었다. 이 검정을 위해, 90 μL의 100 mM 인산나트륨 및 2.5 μL 트리올레인 pH 7.0을 10 μL의 위장 반응물과 혼합하고, 폴리아크릴레이트 96 웰 미세역가 플레이트(COSTAR® 플레이트 #3635; Corning)의 웰에 첨가하고, 37℃에서 30분 내지 1시간 동안 인큐베이션하였다. 반응물을 600 μL ACN:IPA(1:3)로 켄칭시키고, 정화하고, 10 μL를 190 μL 50:50 IPA:MeOH로 희석하고, 1분 동안 진탕한 다음, 10 μL를 190 μL의 50:25:25 H2O:IPA:MeOH에 첨가하여 다시 한번 희석하였다. 이어서, 샘플을 RAPIDFIRE® MS로 분석하였다. 결과는 실시예 4의 표에 제공된다.
실시예 4
리파제 변이체에 대한 스크리닝 결과
상동체 다양성 및 포화 돌연변이유발로부터 생성된 변이체를 실시예 3에 기재된 바와 같이 여러 상이한 조건 하에서 스크리닝하였다. 결과(서열번호 2 기준)는 표 4-1에 제공된다. 이 표에서, 아미노산 차이는 서열번호 2과 비교하여 표시된다.
[표 4-1]
표 4-1에 나타난 결과에 기초하여, 서열번호 94는 단백질 최적화의 다음 반복을 위한 모 서열로 선택되었다. 표 4-1에 나타난 결과에서 확인된 유익한 돌연변이는 골격으로 재조합되었다. 변이체는 실시예 3에 기재된 바와 동일한 조건 하에서 스크리닝되었다. 유일한 차이는 산 도전 스크리닝이 pH 3 대신에 pH 3.5에서 수행되었다는 것이다. 서열번호 94와 비교한 데이터는 표 4-2에 열거된다. 이 표에서, 아미노산 차이는 서열번호 94와 비교하여 표시된다.
[표 4-2]
표 4-2에 나타난 결과에 기초하여, 서열번호 350은 단백질 최적화의 다음 반복을 위한 다음 모 서열로 선택되었다. 표 4-2로부터 확인된 유익한 돌연변이는 골격으로 재조합되었다. 추가로, 상이한 위치에서 포화 돌연변이유발을 통해 서열번호 350을 사용하여 변이체를 구축하였다. 변이체는 실시예 3에 기재된 바와 같이 검정되었고, 결과는 표 4-3에 제공된다. 이 표에서, 아미노산 차이는 서열번호 350과 비교하여 표시된다.
[표 4-3]
표 4-3의 결과에 기초하여, 서열번호 442는 단백질 최적화의 다음 반복을 위한 다음 모 서열로 선택되었다. 표 4-3에 나타난 결과를 기반으로 확인된 유익한 돌연변이를 골격으로 재조합하였다. 추가로, 변이체는 또한 상이한 위치에서 포화 돌연변이유발을 통해 서열번호 442에 구축되었다. 변이체는 실시예 3에 기재된 바와 같이 검정되었고, 결과는 표 4-4에 제공된다. 이 표에서, 아미노산 차이는 서열번호 442와 비교하여 표시된다.
[표 4-4]
표 4-4에 나타난 결과에 기초하여, 서열번호 540은 단백질 최적화의 다음 반복을 위한 다음 모 서열로 선택되었다. 표 4-4에 나타난 결과를 기반으로 확인된 유익한 돌연변이를 골격으로 재결합하였다. 추가로, 변이체는 또한 상이한 위치에서 포화 돌연변이유발을 통해 서열번호 540에 구축되었다. 변이체는 실시예 3에 기술된 바와 같이 검정되었고, 결과는 표 4-5에 제공된다. 이 표에서, 아미노산 차이는 서열번호 540과 비교하여 표시된다.
[표 4-5]
표 4-5에 나타난 결과에 기초하여, 서열번호 646은 단백질 최적화의 다음 반복을 위한 다음 모 서열로 선택되었다. 표 4-5에 나타난 결과를 기반으로 확인된 유익한 돌연변이를 골격으로 재조합하였다. 변이체는 실시예 3에 기재된 바와 같이 검정되었고, 결과는 표 4-6에 제공된다. 이 표에서, 아미노산 차이는 서열번호 646과 비교하여 표시된다.
[표 4-6]
표 4-6에 나타낸 결과에 기초하여, 서열번호 758은 단백질 최적화의 다음 반복을 위한 다음 모 서열로 선택되었다. 표 4-6에 나타난 결과를 기반으로 확인된 유익한 돌연변이를 골격으로 재조합하였다. 변이체는 실시예 3에 기재된 바와 같이 검정되었고, 결과는 표 4-7에 제공된다. 이 표에서, 아미노산 차이는 서열번호 758과 비교하여 표시된다.
[표 4-7]
실시예 5
서열번호 868 상의 개별 돌연변이 변이체에 대한 스크리닝 결과
단일 점 돌연변이는 서열번호 2와 상이한 24개 위치에서 부위 포화 돌연변이유발을 통해 서열번호 868에 구축되었다. 이들 변이체는 실시예 3에 기재된 바와 같이 다양한 전처리를 거친 후 리파제 활성에 대해 검정되었다. 각각의 변이체는 삼중으로 시험되었고, 서열번호 868 및 서열번호 2 둘 다를 참조한 활성 데이터의 분석이 표 5-1에 열거된다.
[표 5-1]
실시예 6
미니어처 돼지에서 외분비 췌장 기능부전의 수술 모델 검증
외분비 췌관 기능부전(EPI)의 수술 모델은 췌관 리게이션에 의해 미니어처 돼지에서 생성되었다. 수술 7일 전부터 시작하여 실험 전반에 걸쳐 3-4개월령의 암컷 SINCLAIRTM 미니어처 돼지에게 고지방식이(HFD, BERTOLLI® 올리브 오일과 함께 10:1(w/w)로 혼합된 싱클레어 표준 식단 S-9)을 1일 1회 공급하였다. 수술 전 연속 3일 및 수술 후 제15, 16, 17일에 24시간에 걸쳐 총 대변 산물을 수집하였다. 각각의 동물로부터의 1일 총 대변 수집물은 대변 순 중량의 대략 1.5배의 소정 부피의 증류수를 첨가하고, 균질화하고, 3 x 50 mL 분취량으로 나누고, 분석할 때까지 -20℃에서 동결함으로써 후속 분석을 위해 준비되었다. 모델은 대변 샘플에서 지방 흡수 계수(CFA, 변형된 반 드 카머(Van de Kamer) 방법에 의함; Van de Kamer, in Seligson (ed), Standard Methods of Clinical Chemistry, volume 2, Academic Press, New York, NY [1958], pp. 34-39) 및 질소 흡수 계수(CNA, 연소 방법에 의한 바리오 막스 CN(Vario Max CN) 기계에 의한 킬달(Kjeldahl) 총 질소; Watson et al., in Peters et al. (eds.) Recommended Methods of Manure Analysis, Univ. of Wisconsin Cooperative Extension Publishing, Publication No. A3769. Madison, WI. [2003], p. 18-24)를 측정하고, 수술 전 및 후에 변화 백분율을 평가함으로써 검증되었다. 수술 전 건강한 미니어처 돼지 CFA 및 CNA는 각각 91.3% ± 1.1 SEM 및 84.2% ± 1.2 SEM이었다. 수술 후 CFA는 53.2%(p < 0.0001, n = 11) 감소되고, CNA는 28.7%(p < 0.0001, n = 11) 감소되어 EPI 연구에 대해 이 수술 모델을 검증하였다.
실시예 7
CREON
®
판크레리파제와 비교한 서열번호 868의 생체내 특징규명
EPI의 검증된 SINCLAIRTM 미니어처 돼지 수술 모델(암컷, 4-5개월령)은 현재 표준 치료(CREON® 판크레리파제; AbbVie, Inc.)와 비교하여 조작된 리파제 변이체(서열번호 868)를 평가하기 위해 생체내 연구로 진행되었다. 투여하기 적어도 8일 전부터 동물에게 3.9 oz. 컵의 무가당 사과소수와 배합된 HFD(BERTOLLI® 브랜드 올리브 오일과 함께 10:1(w/w)로 혼합된 싱클레어 표준 S-9 식단)를 1일 1회 공급하였다. 투여일 동안, 섭취 전에 식이 지방과의 생체외 리파제 상호작용을 최소화하기 위해 각각의 동물에 대해 식사를 순차적으로 준비하였다. 간단히 말해서, HFD는 먼저 깨끗한 사료 용기에 준비되었다. 다음으로, 산성 pH가 초기 효소 활성화로부터 보호되기 때문에, 효소(각각 258,000U 및 252,000U 리파제에 상응하는 7 x 36,000 U CREON® 판크레리파제 캡슐에서 풀링된 0.5 g의 동결건조된 서열번호 868 분말 또는 5.6 g의 미소구체)를 3.9 oz. 컵의 무가당 사과소스와 혼합하였다. 식사당 투여되는 효소의 단위는 USP 리파제 검정(United States Pharmacopeia and National Formulary (USP 42-NF 37). Rockville, MD: United States Pharmacopeial Convention; 2016, 온라인 uspnf.com/uspnf/document/GUID-AC788D41-90A2-4F36-A6E7-769954A9ED09_1_en-US에서 사용 가능) 및 식사에 제공된 총 계산 지방에 의해 결정된 리파제의 비활성을 기반으로 하였다. 이어서, 리파제-사과소스 혼합물을 HFD로 옮기고 빠르게 혼합하였다. 마지막으로, 다음 준비로 넘어가기 전에 사료 용기를 동물들에게 즉시 제공하였다. 2시간 후, 사료 용기를 제거하였으며; 동물은 30분 이내에 전체 식사를 먹었다. 그룹(n = 3)당 미니어처 돼지의 수를 최대화하기 위해 교차 설계가 이용되었다. 이 연구는 각각의 10일 투여 단계 사이에 8일 '세척(washout)'(1일 1회 HFD + 효소가 없는 사과소스)을 포함하였다. 총 대변물은 각각의 단계의 제9, 10 및 11일에 투여 시작 직전 및 투여 후 24시간에 연속 3일 동안 수집되었다. 대변 샘플은 각각의 1일 총 대변 수집물에 대변 순 중량의 대략 1.5배의 소정의 부피의 증류수를 첨가하고, 균질화하고, 3 x 50 mL로 나누고, 분석할 때까지 -20℃에서 동결하고, 나머지는 폐기함으로써 준비되었다. 상기의 모델 검증에 대해 기재된 바와 같이 분석이 완료되었다. CREON® 판크레리파제 및 서열번호 868 둘 다 수술 전 기준선과 크게 상이하지 않은 값으로 유사한 CFA 개선을 초래하였다.
실시예 8
서열번호 868의 생체내 용량 반응 특징규명
EPI의 검증된 SINCLAIRTM 미니어처 돼지 수술 모델(암컷, 4-6개월령)이 추가적인 생체내 서열번호 868 용량 반응 연구에 사용되었다. HFD 및 섭식 요법, 리파제 혼합 프로토콜, 대변 샘플 수집 및 준비, 분석은 상기에서 기재된 바와 동일하였다. 수술 전 값(92.8% +/- 3.2 SD, n = 8)과 비교하여, 투여 직전에 측정된 수술 후 CFA는 39.9% +/- 13.2 SD로 감소되었다(p < 0.0001, n = 8). 서열번호 868은 이전 연구와 연결하기 위해 258,000 U(0.5 g 동결건조 분말)로 투여되었고, 저용량 효능을 평가하기 위해 86,000 U(167 mg) 및 29,000 U(55.5 mg)로 투여되었다. 평가된 모든 용량은 용량 의존적 방식으로 CFA에 긍정적인 영향을 초래하였다. 고용량으로부터 저용량으로 CFA 값은 각각 투여 전과 비교하여 81.3% +/- 3.9 SD(p < 0.0001, n = 3), 74% +/- 3.6 SD(p < 0.0001, n = 3) 및 65.5 % +/- 3.7 SD(p < 0.0001, n = 2)로 증가되었다.
본 출원에 인용된 모든 공개문, 특허, 특허 출원 및 다른 문서는 각각의 개별 공개문, 특허, 특허 출원 또는 다른 문서가 개별적으로 모든 목적을 위해 참조로 포함되는 것으로 표시되는 것과 동일한 정도로 모든 목적을 위해 전체가 참조로 본원에 포함된다.
다양한 특정 실시양태가 예시되고 설명되었지만, 본 발명의 취지 및 범위를 벗어남이 없이 다양한 변경이 이루어질 수 있음을 이해할 것이다.
SEQUENCE LISTING
<110> CODEXIS, INC.
SOCIETE DES PRODUITS NESTLE S.A.
<120> ENGINEERED LIPASE VARIANTS
<130> CX7-187WO2
<140> TO BE ASSIGNED
<141> 2020-08-28
<150> US 62/894,019
<151> 2019-08-30
<160> 1796
<210> 1
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Wild type triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaata a 1161
<210> 2
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Wild type triacylglycerol lipase from Bacillus thermoamylovorans
<400> 2
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 3
<211> 1353
<212> DNA
<213> Artificial Sequence
<220>
<223> Pancreatic Triacylglycerol Lipase from Homo sapiens
<400> 3
atgaaggaag tttgctacga gcgtctgggt tgtttcagcg atgacagccc gtggagcggt 60
atcaccgaac gtccgctgca cattttgccg tggagcccga aagacgttaa cacccgtttc 120
ctgttgtata ccaacgaaaa tccgaacaat tttcaagagg ttgctgcgga tagcagcagc 180
atcagcggta gcaacttcaa gaccaatcgt aaaacccgtt tcattatcca tggttttatt 240
gacaagggtg aagagaactg gctggctaac gtttgcaaga acttgtttaa agttgaaagc 300
gttaactgca tctgtgttga ttggaaaggt ggtagccgta ccggttacac ccaagctagc 360
cagaacattc gtatcgttgg tgctgaagtt gcgtacttcg ttgagtttct gcaaagcgcg 420
tttggttata gcccgagcaa tgttcacgtt attggtcatt ctctgggtgc tcacgctgcg 480
ggtgaggcgg gtcgtcgtac caacggtacc attggtcgta tcaccggtct ggacccggcg 540
gaaccgtgtt tccagggtac cccggagctg gttcgtttgg acccgagcga tgctaagttt 600
gttgacgtta tccacaccga tggtgcgccg attgttccga acctgggttt cggtatgagc 660
caagttgttg gtcatttgga cttctttccg aatggtggtg ttgaaatgcc gggttgcaag 720
aaaaacatcc tgagccagat tgttgatatc gacggtattt gggagggtac ccgtgatttt 780
gctgcgtgta atcacttgcg tagctacaag tactataccg acagcattgt taacccggat 840
ggtttcgctg gttttccgtg cgcgagctat aatgttttca ccgctaacaa atgctttccg 900
tgtccgagcg gtggttgtcc gcaaatgggt cattacgcgg accgttatcc gggtaaaacc 960
aatgatgttg gtcagaaatt ctacctggac accggtgacg ctagcaactt tgcgcgttgg 1020
cgttataagg ttagcgttac cttgagcggt aaaaaggtta ccggtcacat cctggttagc 1080
ttgttcggta acaagggtaa tagcaagcaa tacgaaattt ttaagggtac cctgaaaccg 1140
gacagcaccc atagcaatga gttcgatagc gacgttgatg ttggtgacct gcaaatggtt 1200
aagttcattt ggtacaacaa cgttattaac ccgaccttgc cgcgtgttgg tgctagcaag 1260
attatcgttg aaaccaatgt tggtaaacag ttcaactttt gcagcccgga gaccgttcgt 1320
gaagaggttc tgttgacctt gaccccgtgt taa 1353
<210> 4
<211> 450
<212> PRT
<213> Artificial Sequence
<220>
<223> Pancreatic Triacylglycerol Lipase from Homo sapiens
<400> 4
Met Lys Glu Val Cys Tyr Glu Arg Leu Gly Cys Phe Ser Asp Asp Ser
1 5 10 15
Pro Trp Ser Gly Ile Thr Glu Arg Pro Leu His Ile Leu Pro Trp Ser
20 25 30
Pro Lys Asp Val Asn Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro
35 40 45
Asn Asn Phe Gln Glu Val Ala Ala Asp Ser Ser Ser Ile Ser Gly Ser
50 55 60
Asn Phe Lys Thr Asn Arg Lys Thr Arg Phe Ile Ile His Gly Phe Ile
65 70 75 80
Asp Lys Gly Glu Glu Asn Trp Leu Ala Asn Val Cys Lys Asn Leu Phe
85 90 95
Lys Val Glu Ser Val Asn Cys Ile Cys Val Asp Trp Lys Gly Gly Ser
100 105 110
Arg Thr Gly Tyr Thr Gln Ala Ser Gln Asn Ile Arg Ile Val Gly Ala
115 120 125
Glu Val Ala Tyr Phe Val Glu Phe Leu Gln Ser Ala Phe Gly Tyr Ser
130 135 140
Pro Ser Asn Val His Val Ile Gly His Ser Leu Gly Ala His Ala Ala
145 150 155 160
Gly Glu Ala Gly Arg Arg Thr Asn Gly Thr Ile Gly Arg Ile Thr Gly
165 170 175
Leu Asp Pro Ala Glu Pro Cys Phe Gln Gly Thr Pro Glu Leu Val Arg
180 185 190
Leu Asp Pro Ser Asp Ala Lys Phe Val Asp Val Ile His Thr Asp Gly
195 200 205
Ala Pro Ile Val Pro Asn Leu Gly Phe Gly Met Ser Gln Val Val Gly
210 215 220
His Leu Asp Phe Phe Pro Asn Gly Gly Val Glu Met Pro Gly Cys Lys
225 230 235 240
Lys Asn Ile Leu Ser Gln Ile Val Asp Ile Asp Gly Ile Trp Glu Gly
245 250 255
Thr Arg Asp Phe Ala Ala Cys Asn His Leu Arg Ser Tyr Lys Tyr Tyr
260 265 270
Thr Asp Ser Ile Val Asn Pro Asp Gly Phe Ala Gly Phe Pro Cys Ala
275 280 285
Ser Tyr Asn Val Phe Thr Ala Asn Lys Cys Phe Pro Cys Pro Ser Gly
290 295 300
Gly Cys Pro Gln Met Gly His Tyr Ala Asp Arg Tyr Pro Gly Lys Thr
305 310 315 320
Asn Asp Val Gly Gln Lys Phe Tyr Leu Asp Thr Gly Asp Ala Ser Asn
325 330 335
Phe Ala Arg Trp Arg Tyr Lys Val Ser Val Thr Leu Ser Gly Lys Lys
340 345 350
Val Thr Gly His Ile Leu Val Ser Leu Phe Gly Asn Lys Gly Asn Ser
355 360 365
Lys Gln Tyr Glu Ile Phe Lys Gly Thr Leu Lys Pro Asp Ser Thr His
370 375 380
Ser Asn Glu Phe Asp Ser Asp Val Asp Val Gly Asp Leu Gln Met Val
385 390 395 400
Lys Phe Ile Trp Tyr Asn Asn Val Ile Asn Pro Thr Leu Pro Arg Val
405 410 415
Gly Ala Ser Lys Ile Ile Val Glu Thr Asn Val Gly Lys Gln Phe Asn
420 425 430
Phe Cys Ser Pro Glu Thr Val Arg Glu Glu Val Leu Leu Thr Leu Thr
435 440 445
Pro Cys
450
<210> 5
<211> 1353
<212> DNA
<213> Artificial Sequence
<220>
<223> Pancreatic Triacylglycerol Lipase from Panthera tigris altaica
<400> 5
atgaaggaag tttgcttccc gcgtctgggt tgttttagcg atgacgctcc gtgggcgggt 60
atcgttcaac gtccgctgaa gattttgccg tgggctccga aagacgttaa cacccgtttc 120
ctgttgtaca ccaacgagaa tccggataat tttcaggaat tgaccgctga cgcgagcatt 180
atcaccgata gcaacttcaa gaccaatcgt aaaacccgtt tcattatcca cggttttatc 240
gacaagggtg aagagagctg gctggcgaac atttgcaaaa atttgtttac cgttgagagc 300
gttaactgca tctgtgttga ttggaagagc ggtagccgta ccggttatac ccaagctagc 360
cagaatattc gtatcgttgg tgctgaggtt gcgtacttcg ttgaagttct gaaaagcgcg 420
ttgggttata gcccgagcaa cgttcacatt atcggtcata gcctgggtag ccatgctgcg 480
ggtgaagctg gtcgtcgtac caatggtacc gttggtcgta tcaccggttt ggacccggcg 540
gaaccgtgtt tcgagggtac cccggaactg gttcgtttgg acccgagcga tgctcaattt 600
gttgacgtta ttcacaccga tgctgcgccg attatcccga acatgggttt cggtatgagc 660
cagaatgttg gtcatctgga tttctttccg aacggtggta aagagatgcc gggttgccaa 720
aagaacatct tgagccagat tgttgatatc gacggtattt gggaaggtac ccgtgacttt 780
gttgcgtgta accacctgcg tagctacaag tactatagcg atagcatttt gaatccgacc 840
ggtttcgctg gttttccgtg cgcgagctat agcgttttca ccgctaacaa gtgctttccg 900
tgtccgagcg agggttgtcc gcaaatgggt cattacgcgg accgttttag cggtaaaacc 960
aacggtgtta atcaggtttt ctacctgaac accggtgacg ctagcgattt tgcgcgttgg 1020
cgttataagg ttgctgttac cttgagcggt aaaaaggtta ccggtcacgt tctggttagc 1080
ttgttcggta acaagggtaa tagcaaacaa tacgagattt tcaagggtac cctgcaggcg 1140
gacaacaccc atagcaatga atttgatagc gacgttgatg ttggtgacct gcaaaaggtt 1200
aagttcattt ggtacaacaa cgttattaac ccgaccttgc cgcgtgttgg tgctagcaaa 1260
atcgttgttg aacgtaacga cggtattagc ttcaattttt gcagccagga gaccgttcgt 1320
gaagatgttc tgttgaccct gaccccgtgt taa 1353
<210> 6
<211> 450
<212> PRT
<213> Artificial Sequence
<220>
<223> Pancreatic Triacylglycerol Lipase from Panthera tigris altaica
<400> 6
Met Lys Glu Val Cys Phe Pro Arg Leu Gly Cys Phe Ser Asp Asp Ala
1 5 10 15
Pro Trp Ala Gly Ile Val Gln Arg Pro Leu Lys Ile Leu Pro Trp Ala
20 25 30
Pro Lys Asp Val Asn Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro
35 40 45
Asp Asn Phe Gln Glu Leu Thr Ala Asp Ala Ser Ile Ile Thr Asp Ser
50 55 60
Asn Phe Lys Thr Asn Arg Lys Thr Arg Phe Ile Ile His Gly Phe Ile
65 70 75 80
Asp Lys Gly Glu Glu Ser Trp Leu Ala Asn Ile Cys Lys Asn Leu Phe
85 90 95
Thr Val Glu Ser Val Asn Cys Ile Cys Val Asp Trp Lys Ser Gly Ser
100 105 110
Arg Thr Gly Tyr Thr Gln Ala Ser Gln Asn Ile Arg Ile Val Gly Ala
115 120 125
Glu Val Ala Tyr Phe Val Glu Val Leu Lys Ser Ala Leu Gly Tyr Ser
130 135 140
Pro Ser Asn Val His Ile Ile Gly His Ser Leu Gly Ser His Ala Ala
145 150 155 160
Gly Glu Ala Gly Arg Arg Thr Asn Gly Thr Val Gly Arg Ile Thr Gly
165 170 175
Leu Asp Pro Ala Glu Pro Cys Phe Glu Gly Thr Pro Glu Leu Val Arg
180 185 190
Leu Asp Pro Ser Asp Ala Gln Phe Val Asp Val Ile His Thr Asp Ala
195 200 205
Ala Pro Ile Ile Pro Asn Met Gly Phe Gly Met Ser Gln Asn Val Gly
210 215 220
His Leu Asp Phe Phe Pro Asn Gly Gly Lys Glu Met Pro Gly Cys Gln
225 230 235 240
Lys Asn Ile Leu Ser Gln Ile Val Asp Ile Asp Gly Ile Trp Glu Gly
245 250 255
Thr Arg Asp Phe Val Ala Cys Asn His Leu Arg Ser Tyr Lys Tyr Tyr
260 265 270
Ser Asp Ser Ile Leu Asn Pro Thr Gly Phe Ala Gly Phe Pro Cys Ala
275 280 285
Ser Tyr Ser Val Phe Thr Ala Asn Lys Cys Phe Pro Cys Pro Ser Glu
290 295 300
Gly Cys Pro Gln Met Gly His Tyr Ala Asp Arg Phe Ser Gly Lys Thr
305 310 315 320
Asn Gly Val Asn Gln Val Phe Tyr Leu Asn Thr Gly Asp Ala Ser Asp
325 330 335
Phe Ala Arg Trp Arg Tyr Lys Val Ala Val Thr Leu Ser Gly Lys Lys
340 345 350
Val Thr Gly His Val Leu Val Ser Leu Phe Gly Asn Lys Gly Asn Ser
355 360 365
Lys Gln Tyr Glu Ile Phe Lys Gly Thr Leu Gln Ala Asp Asn Thr His
370 375 380
Ser Asn Glu Phe Asp Ser Asp Val Asp Val Gly Asp Leu Gln Lys Val
385 390 395 400
Lys Phe Ile Trp Tyr Asn Asn Val Ile Asn Pro Thr Leu Pro Arg Val
405 410 415
Gly Ala Ser Lys Ile Val Val Glu Arg Asn Asp Gly Ile Ser Phe Asn
420 425 430
Phe Cys Ser Gln Glu Thr Val Arg Glu Asp Val Leu Leu Thr Leu Thr
435 440 445
Pro Cys
450
<210> 7
<211> 1362
<212> DNA
<213> Artificial Sequence
<220>
<223> Pancreatic Lipase-Related Protein 2 from Homo sapiens
<400> 7
atgaaagaag tttgctatgg tcaactgggt tgtttcagcg atgagaagcc gtgggctggt 60
accttgcagc gtccggttaa actgttgccg tggagcccgg aagatatcga cacccgtttc 120
ctgttgtaca ccaacgagaa tccgaacaat tttcaactga tcaccggtac cgaaccggat 180
accattgagg ctagcaactt ccagctggac cgtaagaccc gtttcattat ccacggtttc 240
ctggataaag cggaagacag ctggccgagc gatatgtgca agaaaatgtt tgaagttgag 300
aaggttaact gcatttgtgt tgactggcgt catggtagcc gtgctatgta cacccaagcg 360
gttcagaata tccgtgttgt tggtgctgag accgcgtttc tgattcaagc tctgagcacc 420
cagctgggtt atagcttgga agatgttcac gttatcggtc attctttggg tgctcacacc 480
gctgcggagg cgggtcgtcg tctgggtggt cgtgttggtc gtattaccgg tctggacccg 540
gctggtccgt gtttccaaga cgaaccggaa gaagttcgtc tggacccgag cgacgcggtt 600
tttgttgatg ttatccacac cgacagcagc ccgattgttc cgagcctggg tttcggtatg 660
agccagaagg ttggtcattt ggacttcttt ccgaacggtg gtaaagagat gccgggttgc 720
aagaaaaatg ttctgagcac cattaccgat atcgacggta tttgggaagg tatcggtggt 780
ttcgttagct gtaaccactt gcgtagcttt gagtactaca gcagcagcgt tctgaatccg 840
gatggtttcc tgggttaccc gtgcgctagc tatgacgaat ttcaagagag caagtgcttt 900
ccgtgtccgg cggaaggttg tccgaaaatg ggtcattacg ctgatcaatt caagggtaaa 960
accagcgcgg ttgaacagac cttctttctg aacaccggcg agagcggtaa ttttaccagc 1020
tggcgttata aggttagcgt taccctgagc ggtaaagaga aagttaacgg ttacattcgt 1080
atcgctttgt atggtagcaa cgaaaatagc aaacaatacg agattttcaa gggtagcctg 1140
aaaccggacg ctagccatac ctgcgcgatc gatgttgact tcaacgttgg taaaatccag 1200
aaggttaagt tcttgtggaa caagcgtggt atcaatctga gcgaaccgaa attgggtgcg 1260
agccaaatta ccgttcagag cggtgaagat ggtaccgagt acaacttttg cagcagcgac 1320
accgttgaag agaatgttct gcagagcttg tatccgtgtt aa 1362
<210> 8
<211> 453
<212> PRT
<213> Artificial Sequence
<220>
<223> Pancreatic Lipase-Related Protein 2 from Homo sapiens
<400> 8
Met Lys Glu Val Cys Tyr Gly Gln Leu Gly Cys Phe Ser Asp Glu Lys
1 5 10 15
Pro Trp Ala Gly Thr Leu Gln Arg Pro Val Lys Leu Leu Pro Trp Ser
20 25 30
Pro Glu Asp Ile Asp Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro
35 40 45
Asn Asn Phe Gln Leu Ile Thr Gly Thr Glu Pro Asp Thr Ile Glu Ala
50 55 60
Ser Asn Phe Gln Leu Asp Arg Lys Thr Arg Phe Ile Ile His Gly Phe
65 70 75 80
Leu Asp Lys Ala Glu Asp Ser Trp Pro Ser Asp Met Cys Lys Lys Met
85 90 95
Phe Glu Val Glu Lys Val Asn Cys Ile Cys Val Asp Trp Arg His Gly
100 105 110
Ser Arg Ala Met Tyr Thr Gln Ala Val Gln Asn Ile Arg Val Val Gly
115 120 125
Ala Glu Thr Ala Phe Leu Ile Gln Ala Leu Ser Thr Gln Leu Gly Tyr
130 135 140
Ser Leu Glu Asp Val His Val Ile Gly His Ser Leu Gly Ala His Thr
145 150 155 160
Ala Ala Glu Ala Gly Arg Arg Leu Gly Gly Arg Val Gly Arg Ile Thr
165 170 175
Gly Leu Asp Pro Ala Gly Pro Cys Phe Gln Asp Glu Pro Glu Glu Val
180 185 190
Arg Leu Asp Pro Ser Asp Ala Val Phe Val Asp Val Ile His Thr Asp
195 200 205
Ser Ser Pro Ile Val Pro Ser Leu Gly Phe Gly Met Ser Gln Lys Val
210 215 220
Gly His Leu Asp Phe Phe Pro Asn Gly Gly Lys Glu Met Pro Gly Cys
225 230 235 240
Lys Lys Asn Val Leu Ser Thr Ile Thr Asp Ile Asp Gly Ile Trp Glu
245 250 255
Gly Ile Gly Gly Phe Val Ser Cys Asn His Leu Arg Ser Phe Glu Tyr
260 265 270
Tyr Ser Ser Ser Val Leu Asn Pro Asp Gly Phe Leu Gly Tyr Pro Cys
275 280 285
Ala Ser Tyr Asp Glu Phe Gln Glu Ser Lys Cys Phe Pro Cys Pro Ala
290 295 300
Glu Gly Cys Pro Lys Met Gly His Tyr Ala Asp Gln Phe Lys Gly Lys
305 310 315 320
Thr Ser Ala Val Glu Gln Thr Phe Phe Leu Asn Thr Gly Glu Ser Gly
325 330 335
Asn Phe Thr Ser Trp Arg Tyr Lys Val Ser Val Thr Leu Ser Gly Lys
340 345 350
Glu Lys Val Asn Gly Tyr Ile Arg Ile Ala Leu Tyr Gly Ser Asn Glu
355 360 365
Asn Ser Lys Gln Tyr Glu Ile Phe Lys Gly Ser Leu Lys Pro Asp Ala
370 375 380
Ser His Thr Cys Ala Ile Asp Val Asp Phe Asn Val Gly Lys Ile Gln
385 390 395 400
Lys Val Lys Phe Leu Trp Asn Lys Arg Gly Ile Asn Leu Ser Glu Pro
405 410 415
Lys Leu Gly Ala Ser Gln Ile Thr Val Gln Ser Gly Glu Asp Gly Thr
420 425 430
Glu Tyr Asn Phe Cys Ser Ser Asp Thr Val Glu Glu Asn Val Leu Gln
435 440 445
Ser Leu Tyr Pro Cys
450
<210> 9
<211> 1143
<212> DNA
<213> Artificial Sequence
<220>
<223> Gastric lipase from Canis lupus familiaris
<400> 9
atgttgttcg gtaaactgca cccgaccaac ccggaagtta ccatgaatat cagccaaatg 60
attacctact ggggttatcc ggctgaagag tacgaagttg ttaccgagga cggttatatt 120
ctgggtatcg atcgtattcc gtacggtcgt aagaacagcg agaatatcgg tcgtcgtccg 180
gttgcgttcc tgcagcatgg tctgttggct agcgcgacca actggatcag caatttgccg 240
aacaatagct tggcttttat tctggctgat gcgggttatg acgtttggct gggtaacagc 300
cgtggtaata cctgggcgcg tcgtaacttg tactatagcc cggacagcgt tgaattttgg 360
gctttcagct ttgatgagat ggcgaaatac gacttgccgg ctaccatcga ttttattctg 420
aagaaaaccg gtcaggataa gttgcactat gttggtcata gccagggtac caccatcggt 480
ttcattgcgt ttagcaccaa cccgaagctg gctaaacgta ttaagacctt ctacgctttg 540
gcgccggttg cgaccgttaa atataccgaa accctgttga acaagttgat gctggttccg 600
agcttcttgt ttaaactgat cttcggtaat aagattttct acccgcacca tttctttgac 660
caatttctgg cgaccgaagt ttgcagccgt gagaccgttg atctgctgtg cagcaacgct 720
ctgttcatta tctgtggttt tgacaccatg aacttgaata tgagccgttt ggatgtttac 780
ctgagccaca acccggctgg taccagcgtt caaaatgttc tgcattggag ccaggcggtt 840
aaaagcggta aattccaggc ttttgactgg ggtagcccgg ttcaaaacat gatgcactat 900
catcagagca tgccgccata ctataatctg accgatatgc acgttccgat cgcggtttgg 960
aacggtggta atgatctgct ggctgacccg catgatgttg acctgttgct gagcaaattg 1020
ccgaacctga tctaccaccg taagattccg ccgtataatc atctggactt tatctgggct 1080
atggatgcgc cgcaagctgt ttacaacgag attgttagca tgatgggtac cgacaacaag 1140
taa 1143
<210> 10
<211> 380
<212> PRT
<213> Artificial Sequence
<220>
<223> Gastric lipase from Canis lupus familiaris
<400> 10
Met Leu Phe Gly Lys Leu His Pro Thr Asn Pro Glu Val Thr Met Asn
1 5 10 15
Ile Ser Gln Met Ile Thr Tyr Trp Gly Tyr Pro Ala Glu Glu Tyr Glu
20 25 30
Val Val Thr Glu Asp Gly Tyr Ile Leu Gly Ile Asp Arg Ile Pro Tyr
35 40 45
Gly Arg Lys Asn Ser Glu Asn Ile Gly Arg Arg Pro Val Ala Phe Leu
50 55 60
Gln His Gly Leu Leu Ala Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro
65 70 75 80
Asn Asn Ser Leu Ala Phe Ile Leu Ala Asp Ala Gly Tyr Asp Val Trp
85 90 95
Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Arg Arg Asn Leu Tyr Tyr
100 105 110
Ser Pro Asp Ser Val Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala
115 120 125
Lys Tyr Asp Leu Pro Ala Thr Ile Asp Phe Ile Leu Lys Lys Thr Gly
130 135 140
Gln Asp Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly
145 150 155 160
Phe Ile Ala Phe Ser Thr Asn Pro Lys Leu Ala Lys Arg Ile Lys Thr
165 170 175
Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Glu Thr Leu
180 185 190
Leu Asn Lys Leu Met Leu Val Pro Ser Phe Leu Phe Lys Leu Ile Phe
195 200 205
Gly Asn Lys Ile Phe Tyr Pro His His Phe Phe Asp Gln Phe Leu Ala
210 215 220
Thr Glu Val Cys Ser Arg Glu Thr Val Asp Leu Leu Cys Ser Asn Ala
225 230 235 240
Leu Phe Ile Ile Cys Gly Phe Asp Thr Met Asn Leu Asn Met Ser Arg
245 250 255
Leu Asp Val Tyr Leu Ser His Asn Pro Ala Gly Thr Ser Val Gln Asn
260 265 270
Val Leu His Trp Ser Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe
275 280 285
Asp Trp Gly Ser Pro Val Gln Asn Met Met His Tyr His Gln Ser Met
290 295 300
Pro Pro Tyr Tyr Asn Leu Thr Asp Met His Val Pro Ile Ala Val Trp
305 310 315 320
Asn Gly Gly Asn Asp Leu Leu Ala Asp Pro His Asp Val Asp Leu Leu
325 330 335
Leu Ser Lys Leu Pro Asn Leu Ile Tyr His Arg Lys Ile Pro Pro Tyr
340 345 350
Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Ala Val Tyr
355 360 365
Asn Glu Ile Val Ser Met Met Gly Thr Asp Asn Lys
370 375 380
<210> 11
<211> 1143
<212> DNA
<213> Artificial Sequence
<220>
<223> Gastric lipase from Leptonychotes weddellii
<400> 11
atgtacttcg gtaaactgca cccgatcagc ccggaagtta ccatgaacat cagccaaatg 60
attacctact ggggttatcc gagcgaagag tacgatgttg ttaccgaaga cggttatatt 120
ctgggtatca atcgtattcc gtacggtaaa aagaacagcg agaatattgg tcgtcgtccg 180
gttgctttcc tgcagcatgg tctgttggct agcgcgacca actggatcgc taatttgccg 240
aacaatagct tggcgtttat tctggctgat gcgggttacg acgtttggct gggtaacagc 300
cgtggtaata cctgggctcg tcgtaacttg tatttcagcc cggacagcgt tgaattttgg 360
gcgttcagct ttgatgagat ggctaaatac gacttgccgg cgaccatcga tttcattctg 420
aagaaaaccg gtcaggataa gttgcactat gttggtcata gccagggtac caccatcggt 480
ttcattgctt ttagcaccaa cccgaagctg gcgaaacgta tcaagacctt ttacgctttg 540
gcgccggttg ctaccgttaa atataccaag agcctgttga acaagctggc gctggttccg 600
agcttcttgt ttaaactgat cttcggtgac aagattttct ttccgcacca attctttgaa 660
cagtttctgg ctaccgaagt ttgcagccgt gagaccgttg ctctgttgtg cagcaacgcg 720
ctgttcatta tctgtggttt tgacaccaag aacttgaatg ttagccgttt ggatgtttac 780
ctgagccaca atccggctgg taccagcgtt caagacgttc tgcattggag ccaggcggtt 840
aaaagcggta aattccaggc tttcgattgg ggtagcccgg cgcaaaacat gctgcactat 900
catcagccga ccccgccgta ctataatttg accgacatgc atgttccgat cgctgtttgg 960
aacggtggta atgatctgct ggcggacccg caagatgttg acctgttgct gagcaaattg 1020
ccgaacctga tctaccacaa gaaaattccg ccgtataatc atctggactt tatttgggct 1080
atggatgcgc cgcaggttat ctacaacgat attgttagca tgatgggcga gaacaataag 1140
taa 1143
<210> 12
<211> 380
<212> PRT
<213> Artificial Sequence
<220>
<223> Gastric lipase from Leptonychotes weddellii
<400> 12
Met Tyr Phe Gly Lys Leu His Pro Ile Ser Pro Glu Val Thr Met Asn
1 5 10 15
Ile Ser Gln Met Ile Thr Tyr Trp Gly Tyr Pro Ser Glu Glu Tyr Asp
20 25 30
Val Val Thr Glu Asp Gly Tyr Ile Leu Gly Ile Asn Arg Ile Pro Tyr
35 40 45
Gly Lys Lys Asn Ser Glu Asn Ile Gly Arg Arg Pro Val Ala Phe Leu
50 55 60
Gln His Gly Leu Leu Ala Ser Ala Thr Asn Trp Ile Ala Asn Leu Pro
65 70 75 80
Asn Asn Ser Leu Ala Phe Ile Leu Ala Asp Ala Gly Tyr Asp Val Trp
85 90 95
Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Arg Arg Asn Leu Tyr Phe
100 105 110
Ser Pro Asp Ser Val Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala
115 120 125
Lys Tyr Asp Leu Pro Ala Thr Ile Asp Phe Ile Leu Lys Lys Thr Gly
130 135 140
Gln Asp Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly
145 150 155 160
Phe Ile Ala Phe Ser Thr Asn Pro Lys Leu Ala Lys Arg Ile Lys Thr
165 170 175
Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu
180 185 190
Leu Asn Lys Leu Ala Leu Val Pro Ser Phe Leu Phe Lys Leu Ile Phe
195 200 205
Gly Asp Lys Ile Phe Phe Pro His Gln Phe Phe Glu Gln Phe Leu Ala
210 215 220
Thr Glu Val Cys Ser Arg Glu Thr Val Ala Leu Leu Cys Ser Asn Ala
225 230 235 240
Leu Phe Ile Ile Cys Gly Phe Asp Thr Lys Asn Leu Asn Val Ser Arg
245 250 255
Leu Asp Val Tyr Leu Ser His Asn Pro Ala Gly Thr Ser Val Gln Asp
260 265 270
Val Leu His Trp Ser Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe
275 280 285
Asp Trp Gly Ser Pro Ala Gln Asn Met Leu His Tyr His Gln Pro Thr
290 295 300
Pro Pro Tyr Tyr Asn Leu Thr Asp Met His Val Pro Ile Ala Val Trp
305 310 315 320
Asn Gly Gly Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Leu Leu
325 330 335
Leu Ser Lys Leu Pro Asn Leu Ile Tyr His Lys Lys Ile Pro Pro Tyr
340 345 350
Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Val Ile Tyr
355 360 365
Asn Asp Ile Val Ser Met Met Gly Glu Asn Asn Lys
370 375 380
<210> 13
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 13
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaaa ggcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 14
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 14
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 15
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 15
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgagta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 16
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 16
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 17
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 17
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 18
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 18
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 19
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 19
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 20
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 20
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 21
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 21
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccattgttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 22
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 22
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Cys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 23
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 23
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctattt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 24
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 24
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Ile Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 25
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 25
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aaggtattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 26
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 26
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 27
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 27
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcgcgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 28
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 28
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Ala Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 29
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 29
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtgtaaata a 1161
<210> 30
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 30
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Cys Lys
385
<210> 31
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 31
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cattgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 32
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 32
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 33
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 33
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 34
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 34
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 35
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 35
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac cgtggttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 36
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 36
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Val
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 37
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 37
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 38
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 38
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 39
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 39
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 40
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 40
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 41
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 41
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag tggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagacgtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 42
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 42
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Thr Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 43
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 43
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatcggtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 44
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 44
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Arg Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 45
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 45
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttcaaa 1158
<210> 46
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 46
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 47
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 47
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaaatgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 48
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 48
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Asn Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 49
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 49
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt gctaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 50
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 50
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Ala Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 51
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 51
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 52
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 52
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 53
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 53
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 54
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 54
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Trp Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 55
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 55
atgcatacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 56
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 56
Met His Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 57
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 57
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 58
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 58
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 59
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 59
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgactaaata a 1161
<210> 60
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 60
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Thr Lys
385
<210> 61
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 61
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 62
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 62
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 63
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 63
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aggaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 64
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 64
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Arg Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 65
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 65
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaaat atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 66
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 66
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 67
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 67
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtattg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 68
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 68
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 69
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 69
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgttgtccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 70
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 70
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Cys Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 71
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 71
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 72
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 72
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 73
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 73
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgcg 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 74
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 74
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Ala Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 75
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 75
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 76
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 76
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 77
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 77
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga ggcgtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 78
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 78
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 79
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 79
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ttgaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 80
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 80
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Leu Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 81
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 81
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 82
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 82
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 83
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 83
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcggaaata a 1161
<210> 84
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 84
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Arg Lys
385
<210> 85
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 85
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgtta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 86
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 86
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 87
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 87
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctttct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 88
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 88
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 89
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 89
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaacttggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 90
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 90
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Leu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 91
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 91
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtgcgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 92
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 92
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Ala Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 93
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 93
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaata a 1161
<210> 94
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 94
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 95
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 95
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagaggtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttatccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 96
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 96
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Arg Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 97
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 97
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 98
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 98
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 99
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 99
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtgggccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 100
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 100
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gly Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 101
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 101
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaggag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 102
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 102
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Arg Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 103
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 103
atggagtcga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 104
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 104
Met Glu Ser Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 105
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 105
atggagcgta gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 106
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 106
Met Glu Arg Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 107
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 107
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaaattggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 108
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 108
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Ile Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 109
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 109
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccggggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 110
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 110
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Arg Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 111
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 111
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctcgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 112
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 112
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Ser Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 113
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 113
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 114
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 114
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 115
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 115
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggttgtg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cgattattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 116
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 116
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Cys Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Ile Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 117
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 117
atggagacct ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 118
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 118
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 119
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 119
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 120
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 120
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 121
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 121
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtcttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 122
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 122
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Ser Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 123
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 123
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acattcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 124
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 124
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Ile His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 125
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 125
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacaaattgt ttcgttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgtttgg tcatccgaat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 126
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 126
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 127
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 127
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aagatgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 128
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 128
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Asp Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 129
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 129
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
agtgagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 130
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 130
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Ser Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 131
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 131
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaggagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 132
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 132
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 133
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 133
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg aggtgtacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 134
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 134
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Val Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 135
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 135
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcgtgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 136
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 136
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Val Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 137
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 137
atggaggcga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 138
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 138
Met Glu Ala Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 139
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 139
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggttcgta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 140
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 140
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ser
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 141
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 141
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aaaggattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 142
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 142
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Arg Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 143
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 143
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 144
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 144
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 145
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 145
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
tttgagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 146
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 146
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Phe Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 147
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 147
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcttgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 148
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 148
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 149
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 149
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 150
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 150
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 151
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 151
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttttgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 152
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 152
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Phe Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 153
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 153
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatacg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 154
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 154
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Thr Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 155
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 155
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tcggttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 156
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 156
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Arg Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 157
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 157
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatag gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 158
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 158
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Arg Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 159
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 159
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 160
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 160
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 161
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 161
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtaatta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 162
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 162
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Asn
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 163
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 163
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt attaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 164
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 164
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Ile Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 165
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 165
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtacgccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 166
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 166
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Thr Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 167
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 167
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtcgtg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 168
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 168
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Arg Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 169
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 169
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaatttggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 170
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 170
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Phe Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 171
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 171
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgtat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 172
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 172
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Tyr Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 173
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 173
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc actggcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 174
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 174
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Trp His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 175
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 175
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cggggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 176
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 176
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Gly Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 177
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 177
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agcagtacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 178
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 178
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Gln Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 179
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 179
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaacacg tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 180
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 180
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Thr Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 181
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 181
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gctttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 182
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 182
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Leu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 183
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 183
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagcatg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 184
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 184
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln His Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 185
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 185
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cactgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 186
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 186
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Thr Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 187
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 187
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 188
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 188
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 189
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 189
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctatct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 190
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 190
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Tyr Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 191
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 191
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttggggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 192
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 192
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Trp Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 193
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 193
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtgttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 194
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 194
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Val Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 195
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 195
atgatgacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 196
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 196
Met Met Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 197
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 197
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
catgacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 198
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 198
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile His Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 199
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 199
atggagggga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 200
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 200
Met Glu Gly Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 201
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 201
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tggataaata a 1161
<210> 202
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 202
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Asp Lys
385
<210> 203
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 203
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a 1161
<210> 204
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 204
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 205
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 205
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagggc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 206
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 206
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Gly His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 207
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 207
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatttat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcaggcag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgtttgg tcatccgact agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 208
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 208
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Ala Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Thr Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 209
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 209
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta tgagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 210
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 210
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Met
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 211
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 211
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gcggagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 212
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 212
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Ala Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 213
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 213
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcgc cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 214
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 214
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Ala Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 215
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 215
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcgctcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 216
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 216
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Ala Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 217
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 217
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
ttggagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 218
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 218
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Leu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 219
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 219
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 220
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 220
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Lys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 221
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 221
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaaaagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 222
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 222
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Lys His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 223
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 223
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctggtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 224
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 224
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Gly Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 225
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 225
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgggtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 226
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 226
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Gly Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 227
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 227
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtt ggagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 228
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 228
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Trp
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 229
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 229
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtc ggagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 230
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 230
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Arg
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 231
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 231
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg agcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 232
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 232
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Glu His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 233
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 233
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcgc cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 234
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 234
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Ala Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 235
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 235
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagctggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 236
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 236
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Ala Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 237
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 237
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agctgtcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 238
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 238
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Cys Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 239
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 239
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtatgta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 240
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 240
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Met Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 241
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 241
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtact 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 242
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 242
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Thr Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 243
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 243
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagtggtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 244
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 244
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Trp Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 245
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 245
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttcaaata a 1161
<210> 246
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 246
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 247
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 247
atggagacct atggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 248
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 248
Met Glu Thr Tyr Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 249
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 249
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agggttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 250
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 250
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Gly Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 251
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 251
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa 1158
<210> 252
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 252
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 253
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 253
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggtg cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 254
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 254
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Val Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 255
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 255
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctacgcgt 1140
gttcatagcc tgtttaaa 1158
<210> 256
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 256
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Thr Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 257
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 257
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tactttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 258
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 258
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Thr Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 259
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 259
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcgtccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 260
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 260
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Arg Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 261
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 261
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtgcgtatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 262
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 262
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Ala Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 263
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 263
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agacttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 264
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 264
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Thr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 265
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 265
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aagttgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 266
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 266
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Val Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 267
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 267
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcaggcag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 268
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 268
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Ala Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 269
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 269
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gcattatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 270
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 270
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu His Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 271
<211> 1161
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 271
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tggctaaata a 1161
<210> 272
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 272
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Ala Lys
385
<210> 273
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 273
atgactacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 274
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 274
Met Thr Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 275
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 275
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaacg gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 276
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 276
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Arg Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 277
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 277
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 278
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 278
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 279
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 279
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 280
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 280
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Asn Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 281
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 281
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa ctcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 282
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 282
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Thr Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 283
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 283
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacttattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 284
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 284
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 285
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 285
atggagacca tgggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 286
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 286
Met Glu Thr Met Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 287
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 287
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tgctagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggctatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 288
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 288
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Ala Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 289
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 289
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taatcagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 290
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 290
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Asn Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 291
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 291
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 292
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 292
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Asn Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 293
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 293
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cgatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 294
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 294
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Asp Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 295
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 295
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggcctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttctgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 296
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 296
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Ser Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 297
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 297
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
agcgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 298
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 298
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile
85 90 95
Tyr Gly Gln Trp Ser Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Asn Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 299
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 299
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
gttcaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 300
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 300
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Val Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 301
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 301
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctacgtt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 302
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 302
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Val Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 303
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 303
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggcg cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 304
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 304
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Ala Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 305
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 305
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 306
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 306
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 307
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 307
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgttg atggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 308
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 308
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 309
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 309
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgaggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 310
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 310
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Glu Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 311
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 311
atggagacgt ggggtaacga ttatccgatt gctctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 312
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 312
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Ala Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 313
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 313
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 314
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 314
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 315
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 315
atggagtcgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 316
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 316
Met Glu Ser Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 317
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 317
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 318
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 318
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 319
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 319
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 320
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 320
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 321
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 321
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gccagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 322
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 322
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 323
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 323
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 324
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 324
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 325
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 325
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 326
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 326
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 327
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 327
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 328
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 328
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 329
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 329
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 330
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 330
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 331
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 331
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 332
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 332
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 333
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 333
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 334
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 334
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 335
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 335
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 336
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 336
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 337
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 337
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 338
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 338
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 339
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 339
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 340
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 340
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 341
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 341
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 342
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 342
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 343
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 343
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 344
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 344
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 345
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 345
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 346
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 346
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 347
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 347
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 348
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 348
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 349
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 349
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 350
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 350
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 351
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 351
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 352
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 352
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 353
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 353
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 354
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 354
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 355
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 355
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 356
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 356
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 357
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 357
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 358
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 358
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 359
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 359
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 360
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 360
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 361
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 361
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 362
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 362
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 363
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 363
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 364
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 364
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 365
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 365
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 366
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 366
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 367
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 367
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gccagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 368
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 368
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 369
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 369
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 370
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 370
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 371
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 371
atggagtcgt cgggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 372
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 372
Met Glu Ser Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 373
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 373
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 374
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 374
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 375
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 375
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 376
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 376
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 377
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 377
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 378
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 378
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 379
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 379
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 380
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 380
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 381
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 381
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 382
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 382
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 383
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 383
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 384
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 384
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 385
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 385
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 386
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 386
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 387
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 387
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 388
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 388
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 389
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 389
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 390
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 390
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 391
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 391
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 392
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 392
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 393
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 393
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 394
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 394
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 395
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 395
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tccgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 396
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 396
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Pro Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 397
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 397
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 398
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 398
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 399
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 399
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 400
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 400
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 401
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 401
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcttcct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aagggattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 402
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 402
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 403
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 403
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 404
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 404
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 405
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 405
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 406
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 406
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 407
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 407
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 408
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 408
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 409
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 409
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 410
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 410
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 411
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 411
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctttct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aagggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aaaaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 412
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 412
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 413
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 413
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 414
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 414
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 415
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 415
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gatgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 416
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 416
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 417
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 417
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 418
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 418
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 419
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 419
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtgattgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 420
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 420
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Asp Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 421
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 421
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 422
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 422
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 423
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 423
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 424
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 424
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 425
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 425
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccggg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 426
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 426
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 427
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 427
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaaaagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 428
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 428
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 429
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 429
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagc ggtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 430
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 430
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Arg Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 431
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 431
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggggttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 432
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 432
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 433
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 433
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tacgagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 434
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 434
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Thr
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 435
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 435
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagctggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 436
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 436
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Trp Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 437
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 437
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaggg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 438
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 438
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 439
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 439
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 440
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 440
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 441
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 441
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 442
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 442
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 443
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 443
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 444
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 444
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Asn Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 445
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 445
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 446
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 446
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 447
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 447
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tgcgagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 448
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 448
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Ala
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 449
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 449
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 450
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 450
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 451
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 451
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 452
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 452
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 453
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 453
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaagggt gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 454
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 454
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Gly Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 455
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 455
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 456
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 456
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Asn Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 457
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 457
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 458
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 458
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 459
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 459
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 460
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 460
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 461
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 461
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 462
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 462
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 463
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 463
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaaccc ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 464
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 464
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr His Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 465
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 465
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 466
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 466
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 467
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 467
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgga tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 468
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 468
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 469
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 469
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac aggccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 470
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 470
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Arg Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 471
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 471
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acgcggacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 472
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 472
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 473
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 473
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ctatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 474
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 474
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Ala
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 475
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 475
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtaggat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 476
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 476
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Arg
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 477
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 477
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 478
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 478
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 479
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 479
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttgtgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 480
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 480
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Val Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 481
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 481
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttatgagcc tgccgaaa 1158
<210> 482
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 482
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val Met Ser Leu
370 375 380
Pro Lys
385
<210> 483
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 483
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct ttgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 484
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 484
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Leu Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 485
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 485
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttatgagcc tgccgaaa 1158
<210> 486
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 486
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val Met Ser Leu
370 375 380
Pro Lys
385
<210> 487
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 487
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatcttacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 488
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 488
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Leu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 489
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 489
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcagtg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 490
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 490
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Ser Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 491
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 491
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatacgacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 492
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 492
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Thr Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 493
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 493
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttctgcacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 494
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 494
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Leu His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 495
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 495
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 496
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 496
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 497
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 497
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt cagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 498
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 498
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 499
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 499
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcgggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 500
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 500
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Gly Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 501
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 501
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 502
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 502
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 503
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 503
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 504
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 504
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 505
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 505
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtctta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 506
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 506
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 507
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 507
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtacgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 508
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 508
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Thr
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 509
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 509
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtacta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 510
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 510
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Thr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 511
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 511
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta taattactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 512
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 512
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Asn Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 513
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 513
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtagtat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 514
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 514
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ser
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 515
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 515
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag gcaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 516
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 516
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Arg Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 517
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 517
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cgagatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 518
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 518
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Glu Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 519
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 519
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 520
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 520
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 521
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 521
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 522
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 522
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Asn Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 523
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 523
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 524
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 524
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 525
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 525
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 526
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 526
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 527
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 527
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 528
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 528
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 529
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 529
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtactg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 530
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 530
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 531
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 531
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 532
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 532
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 533
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 533
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 534
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 534
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 535
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 535
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 536
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 536
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 537
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 537
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacttgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 538
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 538
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 539
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 539
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 540
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 540
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 541
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 541
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt tcatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 542
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 542
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 543
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 543
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 544
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 544
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 545
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 545
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagccgcg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 546
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 546
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 547
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 547
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aatatgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 548
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 548
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 549
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 549
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaaaaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 550
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 550
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 551
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 551
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgttg atggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 552
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 552
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 553
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 553
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttttgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 554
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 554
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 555
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 555
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 556
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 556
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 557
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 557
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcgtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 558
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 558
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 559
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 559
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggggtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 560
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 560
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 561
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 561
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 562
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 562
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 563
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 563
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 564
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 564
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 565
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 565
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 566
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 566
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 567
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 567
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 568
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 568
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 569
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 569
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgcattat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 570
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 570
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu His Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 571
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 571
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttgggacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 572
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 572
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Trp Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 573
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 573
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 574
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 574
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 575
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 575
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 576
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 576
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 577
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 577
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 578
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 578
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 579
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 579
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cgatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 580
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 580
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Asp Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 581
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 581
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagccgcg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 582
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 582
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 583
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 583
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 584
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 584
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 585
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 585
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
caggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 586
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 586
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Gln Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 587
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 587
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 588
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 588
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 589
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 589
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 590
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 590
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 591
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 591
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cccggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 592
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 592
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Pro Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 593
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 593
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ctgtgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 594
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 594
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Cys Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 595
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 595
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctggcgta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 596
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 596
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Ala Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 597
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 597
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc ttgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 598
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 598
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 599
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 599
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcatgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 600
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 600
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala His Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 601
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 601
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgtcgta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 602
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 602
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Ser Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 603
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 603
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgcggta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 604
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 604
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Arg Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 605
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 605
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 606
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 606
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 607
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 607
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaaaaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 608
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 608
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 609
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 609
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 610
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 610
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 611
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 611
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtt tgagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 612
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 612
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Leu
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 613
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 613
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggtt cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 614
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 614
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Val Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 615
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 615
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatccgac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 616
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 616
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Pro Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 617
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 617
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 618
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 618
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 619
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 619
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac cctggttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 620
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 620
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Leu
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 621
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 621
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 622
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 622
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 623
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 623
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 624
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 624
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 625
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 625
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 626
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 626
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 627
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 627
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 628
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 628
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 629
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 629
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa ctcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 630
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 630
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Thr Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 631
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 631
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 632
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 632
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 633
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 633
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 634
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 634
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 635
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 635
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 636
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 636
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 637
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 637
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 638
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 638
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 639
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 639
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 640
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 640
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 641
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 641
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 642
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 642
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 643
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 643
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 644
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 644
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 645
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 645
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 646
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 646
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 647
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 647
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 648
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 648
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 649
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 649
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 650
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 650
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 651
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 651
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 652
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 652
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 653
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 653
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 654
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 654
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 655
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 655
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 656
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 656
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 657
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 657
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 658
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 658
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 659
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 659
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 660
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 660
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 661
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 661
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 662
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 662
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 663
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 663
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 664
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 664
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 665
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 665
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 666
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 666
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 667
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 667
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cacataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 668
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 668
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 669
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 669
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 670
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 670
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 671
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 671
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 672
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 672
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 673
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 673
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 674
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 674
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 675
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 675
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 676
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 676
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 677
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 677
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 678
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 678
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 679
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 679
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 680
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 680
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 681
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 681
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 682
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 682
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 683
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 683
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 684
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 684
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 685
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 685
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 686
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 686
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 687
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 687
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 688
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 688
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 689
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 689
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtacg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 690
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 690
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Thr Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 691
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 691
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 692
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 692
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 693
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 693
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 694
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 694
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 695
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 695
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 696
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 696
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 697
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 697
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 698
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 698
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 699
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 699
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 700
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 700
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 701
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 701
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 702
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 702
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 703
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 703
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tcttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 704
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 704
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 705
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 705
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 706
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 706
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ile Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 707
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 707
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 708
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 708
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 709
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 709
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 710
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 710
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 711
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 711
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 712
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 712
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 713
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 713
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 714
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 714
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 715
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 715
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtacg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 716
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 716
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Thr Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 717
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 717
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgttg atggtaatta ttacccgatt 840
ggtaccatac acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 718
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 718
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Ile His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 719
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 719
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggcagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 720
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 720
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 721
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 721
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 722
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 722
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 723
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 723
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 724
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 724
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 725
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 725
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggcagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata gcggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 726
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 726
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Ser Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 727
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 727
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 728
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 728
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 729
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 729
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 730
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 730
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 731
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 731
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 732
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 732
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 733
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 733
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctactcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 734
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 734
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Ser Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 735
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 735
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccact 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 736
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 736
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Thr
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 737
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 737
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 738
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 738
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 739
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 739
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gttgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 740
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 740
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Val Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 741
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 741
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 742
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 742
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 743
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 743
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 744
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 744
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 745
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 745
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccggcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 746
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 746
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Arg Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 747
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 747
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggttttcaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 748
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 748
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Phe Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 749
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 749
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 750
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 750
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 751
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 751
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaggaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 752
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 752
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 753
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 753
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 754
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 754
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 755
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 755
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 756
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 756
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 757
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 757
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 758
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 758
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 759
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 759
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 760
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 760
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 761
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 761
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 762
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 762
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 763
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 763
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 764
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 764
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 765
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 765
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 766
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 766
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 767
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 767
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 768
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 768
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 769
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 769
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 770
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 770
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 771
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 771
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 772
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 772
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 773
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 773
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 774
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 774
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 775
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 775
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggaggg cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 776
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 776
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Gly Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 777
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 777
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 778
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 778
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 779
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 779
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 780
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 780
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 781
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 781
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 782
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 782
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 783
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 783
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 784
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 784
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 785
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 785
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 786
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 786
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 787
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 787
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 788
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 788
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 789
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 789
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 790
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 790
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 791
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 791
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 792
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 792
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 793
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 793
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 794
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 794
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 795
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 795
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 796
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 796
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 797
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 797
atggagacca gcggtaacga ttatccgatt gttctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 798
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 798
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 799
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 799
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 800
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 800
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 801
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 801
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 802
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 802
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 803
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 803
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 804
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 804
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 805
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 805
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 806
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 806
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 807
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 807
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc gcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 808
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 808
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ala Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 809
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 809
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccggtgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 810
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 810
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Val Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 811
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 811
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 812
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 812
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 813
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 813
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat tcctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatcactgc cgctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 814
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 814
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile Thr Ala Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 815
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 815
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 816
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 816
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 817
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 817
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 818
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 818
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 819
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 819
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 820
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 820
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 821
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 821
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 822
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 822
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 823
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 823
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 824
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 824
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 825
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 825
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
ggtaccatgc acccgctgtt tacgttgatt agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtattgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 826
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 826
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 827
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 827
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 828
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 828
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 829
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 829
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 830
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 830
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 831
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 831
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc gcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 832
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 832
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ala Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 833
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 833
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 834
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 834
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 835
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 835
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 836
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 836
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 837
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 837
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattct gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 838
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 838
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Leu Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 839
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 839
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
tcgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 840
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 840
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Ser Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 841
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 841
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccggtgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 842
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 842
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Val Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 843
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 843
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 844
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 844
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 845
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 845
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 846
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 846
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 847
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 847
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 848
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 848
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 849
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 849
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 850
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 850
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 851
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 851
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 852
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 852
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 853
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 853
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt gagcttcgtc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ctatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 854
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 854
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Phe
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Val Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 855
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 855
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
tcgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 856
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 856
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Phe
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Ser Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 857
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 857
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 858
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 858
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 859
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 859
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 860
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 860
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 861
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 861
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 862
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 862
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 863
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 863
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattct gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ctatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 864
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 864
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Leu Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 865
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 865
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt tacgttgatt agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtattgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 866
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 866
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 867
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 867
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 868
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 868
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 869
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 869
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 870
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 870
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 871
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 871
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 872
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 872
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 873
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 873
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccaaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 874
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 874
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 875
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 875
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tacgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccaaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 876
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 876
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 877
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 877
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 878
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 878
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 879
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 879
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg ttcgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagtcgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 880
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 880
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Ser Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 881
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 881
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg ttcgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 882
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 882
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 883
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 883
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 884
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 884
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 885
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 885
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag cgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 886
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 886
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Ala Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 887
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 887
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 888
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 888
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Cys Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 889
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 889
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 890
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 890
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Asp Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 891
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 891
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag agctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 892
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 892
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Glu Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 893
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 893
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 894
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 894
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 895
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 895
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 896
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 896
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Gly Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 897
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 897
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 898
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 898
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu His Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 899
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 899
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 900
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 900
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Ile Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 901
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 901
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa agctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 902
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 902
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Lys Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 903
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 903
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 904
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 904
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Leu Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 905
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 905
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 906
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 906
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Asn Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 907
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 907
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac ctctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 908
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 908
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Pro Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 909
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 909
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac aactgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 910
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 910
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Gln Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 911
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 911
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa ggctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 912
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 912
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Arg Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 913
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 913
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 914
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 914
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Ser Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 915
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 915
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa cgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 916
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 916
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Thr Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 917
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 917
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 918
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 918
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Val Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 919
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 919
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ggctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 920
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 920
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Trp Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 921
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 921
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 922
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 922
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Tyr Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 923
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 923
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 924
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 924
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 925
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 925
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtgttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 926
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 926
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Cys Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 927
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 927
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggattatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 928
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 928
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Asp Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 929
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 929
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 930
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 930
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Glu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 931
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 931
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 932
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 932
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 933
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 933
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gcactatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 934
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 934
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu His Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 935
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 935
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gatttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 936
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 936
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ile Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 937
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 937
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 938
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 938
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Lys Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 939
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 939
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gctgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 940
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 940
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Leu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 941
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 941
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gatgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 942
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 942
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Met Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 943
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 943
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaactatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 944
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 944
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Asn Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 945
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 945
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gccgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 946
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 946
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Pro Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 947
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 947
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gcagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 948
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 948
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gln Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 949
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 949
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaggtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 950
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 950
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Arg Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 951
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 951
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gagttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 952
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 952
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Ser Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 953
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 953
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gacttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 954
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 954
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Thr Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 955
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 955
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggtgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 956
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 956
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Val Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 957
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 957
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtggtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 958
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 958
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Trp Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 959
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 959
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtattatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 960
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 960
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Tyr Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 961
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 961
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 962
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 962
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Ala Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 963
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 963
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactgtgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 964
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 964
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Cys Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 965
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 965
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgacgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 966
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 966
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Asp Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 967
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 967
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 968
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 968
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Glu Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 969
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 969
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 970
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 970
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Phe Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 971
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 971
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacggggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 972
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 972
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Gly Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 973
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 973
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctaccacgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 974
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 974
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
His Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 975
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 975
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacatcgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 976
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 976
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Ile Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 977
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 977
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacaaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 978
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 978
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Lys Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 979
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 979
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacctggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 980
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 980
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Leu Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 981
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 981
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacatggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 982
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 982
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Met Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 983
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 983
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacaatgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 984
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 984
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Asn Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 985
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 985
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacccggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 986
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 986
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Pro Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 987
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 987
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctaccaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 988
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 988
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Gln Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 989
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 989
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacagggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 990
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 990
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Arg Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 991
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 991
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 992
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 992
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Ser Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 993
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 993
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 994
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 994
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 995
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 995
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactgggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 996
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 996
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Trp Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 997
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 997
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactatgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 998
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 998
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Tyr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 999
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 999
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgct ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1000
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1000
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Ala Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1001
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1001
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttgt ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1002
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1002
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Cys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1003
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1003
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgac ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1004
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1004
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Asp Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1005
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1005
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1006
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1006
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Glu Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1007
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1007
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattttt ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1008
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1008
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Phe Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1009
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1009
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattggg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1010
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1010
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Gly Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1011
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1011
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattatc ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1012
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1012
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Ile Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1013
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1013
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1014
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1014
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1015
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1015
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattctg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1016
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1016
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Leu Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1017
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1017
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattatg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1018
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1018
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Met Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1019
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1019
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1020
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1020
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1021
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1021
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattccg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1022
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1022
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Pro Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1023
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1023
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1024
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1024
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Gln Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1025
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1025
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcgg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1026
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1026
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Arg Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1027
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1027
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttcg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1028
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1028
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Ser Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1029
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1029
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattacg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1030
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1030
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Thr Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1031
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1031
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgtg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1032
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1032
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Val Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1033
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1033
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttgg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1034
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1034
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Trp Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1035
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1035
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1036
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1036
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile Tyr Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1037
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1037
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgcgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1038
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1038
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Ala Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1039
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1039
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctgtggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1040
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1040
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Cys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1041
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1041
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgatggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1042
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1042
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Asp Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1043
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1043
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctttggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1044
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1044
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Phe Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1045
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1045
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgggggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1046
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1046
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Gly Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1047
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1047
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccacggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1048
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1048
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe His Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1049
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1049
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcattggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1050
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1050
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Ile Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1051
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1051
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaagggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1052
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1052
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Lys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1053
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1053
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcttgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1054
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1054
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Leu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1055
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1055
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcatgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1056
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1056
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Met Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1057
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1057
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaacggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1058
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1058
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Asn Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1059
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1059
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccctggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1060
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1060
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Pro Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1061
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1061
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccagggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1062
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1062
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Gln Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1063
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1063
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccgtggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1064
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1064
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Arg Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1065
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1065
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctcgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1066
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1066
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Ser Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1067
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1067
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcacgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1068
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1068
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1069
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1069
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgtaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1070
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1070
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Val Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1071
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1071
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctggggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1072
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1072
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Trp Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1073
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1073
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctatggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1074
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1074
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Tyr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1075
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1075
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gcggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1076
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1076
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ala Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1077
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1077
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tgtgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1078
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1078
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Cys Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1079
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1079
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1080
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1080
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Asp Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1081
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1081
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gaggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1082
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1082
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Glu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1083
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1083
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttcgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1084
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1084
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Phe Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1085
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1085
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ggtgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1086
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1086
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Gly Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1087
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1087
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
catgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1088
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1088
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
His Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1089
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1089
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
aaggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1090
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1090
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Lys Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1091
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1091
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1092
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1092
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1093
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1093
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
atggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1094
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1094
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Met Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1095
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1095
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
aatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1096
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1096
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Asn Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1097
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1097
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ccggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1098
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1098
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Pro Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1099
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1099
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
caggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1100
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1100
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Gln Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1101
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1101
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
cgggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1102
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1102
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Arg Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1103
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1103
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tctgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1104
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1104
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ser Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1105
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1105
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
actgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1106
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1106
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Thr Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1107
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1107
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gtggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1108
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1108
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Val Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1109
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1109
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tgggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1110
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1110
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Trp Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1111
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1111
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1112
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1112
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Tyr Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1113
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1113
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag ctctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1114
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1114
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ala Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1115
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1115
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1116
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1116
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Cys Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1117
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1117
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1118
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1118
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Asp Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1119
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1119
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1120
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1120
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1121
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1121
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat ttctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1122
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1122
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Phe Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1123
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1123
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1124
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1124
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1125
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1125
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1126
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1126
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1127
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1127
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa ttctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1128
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1128
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ile Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1129
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1129
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1130
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1130
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Lys Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1131
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1131
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1132
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1132
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Leu Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1133
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1133
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1134
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1134
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Met Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1135
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1135
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1136
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1136
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Asn Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1137
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1137
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac ctctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1138
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1138
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Pro Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1139
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1139
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1140
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1140
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gln Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1141
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1141
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1142
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1142
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ser Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1143
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1143
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa cgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1144
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1144
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Thr Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1145
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1145
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1146
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1146
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Val Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1147
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1147
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1148
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1148
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Trp Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1149
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1149
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1150
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1150
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Tyr Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1151
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1151
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgctattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1152
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1152
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ala Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1153
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1153
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttgtattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1154
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1154
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Cys Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1155
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1155
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1156
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1156
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Asp Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1157
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1157
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1158
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1158
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Glu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1159
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1159
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttttattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1160
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1160
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Phe Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1161
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1161
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtggtattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1162
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1162
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gly Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1163
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1163
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1164
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1164
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg His Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1165
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1165
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtattattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1166
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1166
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ile Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1167
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1167
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtaagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1168
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1168
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Lys Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1169
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1169
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtatgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1170
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1170
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Met Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1171
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1171
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtaatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1172
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1172
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Asn Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1173
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1173
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcctattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1174
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1174
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Pro Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1175
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1175
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1176
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1176
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1177
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1177
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1178
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1178
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Arg Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1179
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1179
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttcgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1180
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1180
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ser Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1181
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1181
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtactattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1182
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1182
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Thr Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1183
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1183
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgtgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1184
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1184
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Val Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1185
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1185
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1186
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1186
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Trp Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1187
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1187
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1188
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1188
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Tyr Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1189
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1189
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgtgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1190
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1190
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Cys Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1191
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1191
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1192
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1192
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Asp Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1193
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1193
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1194
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1194
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Glu Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1195
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1195
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttttgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1196
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1196
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Phe Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1197
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1197
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctggggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1198
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1198
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Gly Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1199
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1199
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1200
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1200
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala His Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1201
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1201
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctatcgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1202
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1202
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ile Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1203
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1203
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctaaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1204
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1204
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Lys Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1205
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1205
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctctggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1206
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1206
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Leu Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1207
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1207
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctatggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1208
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1208
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Met Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1209
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1209
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctaacgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1210
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1210
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Asn Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1211
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1211
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctccggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1212
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1212
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Pro Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1213
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1213
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1214
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1214
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Gln Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1215
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1215
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctagggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1216
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1216
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Arg Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1217
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1217
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctagtgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1218
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1218
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1219
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1219
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctacggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1220
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1220
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Thr Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1221
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1221
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgtggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1222
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1222
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Val Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1223
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1223
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1224
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1224
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Trp Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1225
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1225
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1226
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1226
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Tyr Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1227
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1227
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgcga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1228
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1228
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Ala Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1229
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1229
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttgta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1230
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1230
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Cys Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1231
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1231
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1232
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1232
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Asp Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1233
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1233
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1234
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1234
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Glu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1235
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1235
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtttta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1236
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1236
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Phe Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1237
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1237
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtggta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1238
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1238
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gly Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1239
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1239
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1240
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1240
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
His Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1241
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1241
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtatta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1242
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1242
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Ile Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1243
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1243
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtaaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1244
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1244
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Lys Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1245
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1245
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtatga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1246
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1246
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Met Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1247
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1247
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtaata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1248
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1248
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Asn Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1249
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1249
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtccga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1250
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1250
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Pro Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1251
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1251
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1252
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1252
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1253
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1253
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcgga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1254
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1254
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Arg Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1255
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1255
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtagta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1256
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1256
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Ser Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1257
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1257
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtacta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1258
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1258
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Thr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1259
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1259
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgtta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1260
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1260
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Val Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1261
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1261
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttgga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1262
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1262
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Trp Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1263
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1263
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1264
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1264
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Tyr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1265
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1265
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgcgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1266
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1266
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Ala Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1267
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1267
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttgtaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1268
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1268
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Cys Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1269
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1269
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgataccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1270
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1270
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Asp Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1271
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1271
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgagaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1272
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1272
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Glu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1273
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1273
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttttaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1274
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1274
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Phe Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1275
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1275
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1276
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1276
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Gly Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1277
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1277
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttcacaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1278
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1278
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
His Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1279
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1279
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttattaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1280
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1280
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Ile Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1281
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1281
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaaaaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1282
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1282
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Lys Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1283
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1283
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1284
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1284
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1285
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1285
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaacaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1286
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1286
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Asn Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1287
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1287
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttccgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1288
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1288
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Pro Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1289
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1289
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttcaaaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1290
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1290
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Gln Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1291
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1291
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1292
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1292
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Arg Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1293
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1293
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttcgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1294
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1294
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Ser Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1295
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1295
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttacgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1296
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1296
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Thr Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1297
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1297
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgtgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1298
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1298
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Val Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1299
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1299
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1300
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1300
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Trp Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1301
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1301
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttataccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1302
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1302
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Tyr Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1303
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1303
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gcgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1304
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1304
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Ala Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1305
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1305
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tgtagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1306
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1306
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Cys Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1307
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1307
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1308
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1308
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Asp Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1309
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1309
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tttagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1310
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1310
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Phe Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1311
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1311
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1312
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1312
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Gly Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1313
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1313
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cacagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1314
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1314
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp His Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1315
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1315
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg attagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1316
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1316
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Ile Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1317
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1317
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aaaagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1318
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1318
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Lys Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1319
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1319
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg ctgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1320
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1320
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Leu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1321
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1321
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg atgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1322
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1322
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Met Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1323
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1323
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1324
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1324
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Asn Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1325
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1325
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cctagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1326
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1326
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Pro Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1327
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1327
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1328
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1328
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1329
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1329
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1330
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1330
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Arg Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1331
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1331
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg agtagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1332
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1332
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Ser Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1333
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1333
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg acgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1334
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1334
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Thr Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1335
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1335
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gttagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1336
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1336
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Val Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1337
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1337
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1338
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1338
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Trp Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1339
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1339
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1340
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1340
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Tyr Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1341
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1341
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgctg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1342
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1342
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Ala Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1343
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1343
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1344
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1344
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Cys Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1345
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1345
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgatg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1346
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1346
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Asp Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1347
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1347
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1348
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1348
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Glu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1349
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1349
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttcg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1350
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1350
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Phe Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1351
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1351
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgggg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1352
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1352
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Gly Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1353
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1353
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccatg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1354
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1354
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr His Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1355
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1355
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccattg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1356
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1356
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Ile Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1357
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1357
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1358
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1358
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Lys Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1359
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1359
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1360
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1360
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1361
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1361
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccatgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1362
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1362
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Met Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1363
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1363
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaacg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1364
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1364
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Asn Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1365
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1365
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccccgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1366
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1366
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Pro Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1367
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1367
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1368
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1368
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Gln Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1369
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1369
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccagtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1370
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1370
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Ser Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1371
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1371
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccacgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1372
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1372
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Thr Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1373
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1373
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgtgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1374
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1374
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Val Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1375
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1375
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctggg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1376
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1376
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Trp Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1377
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1377
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctacg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1378
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1378
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Tyr Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1379
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1379
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggcgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1380
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1380
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ala Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1381
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1381
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtgcaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1382
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1382
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Cys Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1383
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1383
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1384
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1384
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Asp Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1385
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1385
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggagaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1386
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1386
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Glu Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1387
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1387
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtttaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1388
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1388
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Phe Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1389
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1389
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1390
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1390
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Gly Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1391
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1391
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1392
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1392
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp His Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1393
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1393
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggattaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1394
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1394
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ile Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1395
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1395
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggaaaaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1396
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1396
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Lys Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1397
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1397
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcttaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1398
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1398
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Leu Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1399
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1399
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1400
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1400
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1401
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1401
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggaataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1402
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1402
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Asn Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1403
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1403
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggccgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1404
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1404
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Pro Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1405
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1405
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcagaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1406
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1406
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Gln Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1407
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1407
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1408
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1408
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Arg Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1409
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1409
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtctaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1410
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1410
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ser Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1411
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1411
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggactaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1412
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1412
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Thr Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1413
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1413
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1414
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1414
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Trp Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1415
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1415
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1416
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1416
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Tyr Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1417
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1417
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gcggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1418
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1418
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ala Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1419
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1419
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tgtgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1420
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1420
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Cys Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1421
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1421
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gaggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1422
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1422
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Glu Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1423
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1423
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tttgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1424
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1424
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Phe Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1425
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1425
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1426
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1426
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1427
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1427
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt catgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1428
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1428
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg His Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1429
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1429
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt attgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1430
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1430
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ile Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1431
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1431
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt aaggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1432
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1432
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Lys Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1433
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1433
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ttggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1434
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1434
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Leu Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1435
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1435
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt atggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1436
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1436
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Met Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1437
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1437
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt aatgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1438
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1438
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asn Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1439
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1439
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ccggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1440
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1440
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Pro Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1441
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1441
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt caggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1442
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1442
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gln Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1443
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1443
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt cgggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1444
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1444
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Arg Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1445
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1445
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tcggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1446
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1446
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ser Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1447
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1447
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt acggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1448
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1448
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Thr Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1449
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1449
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gttgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1450
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1450
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Val Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1451
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1451
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tgggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1452
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1452
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Trp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1453
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1453
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tatgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1454
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1454
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Tyr Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1455
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1455
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggc gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1456
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1456
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ala Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1457
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1457
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtg caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1458
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1458
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Cys Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1459
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1459
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgga taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1460
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1460
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Asp Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1461
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1461
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgga gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1462
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1462
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Glu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1463
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1463
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1464
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1464
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1465
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1465
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggg gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1466
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1466
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Gly Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1467
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1467
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgca taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1468
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1468
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu His Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1469
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1469
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgat caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1470
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1470
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ile Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1471
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1471
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgaa gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1472
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1472
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Lys Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1473
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1473
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgat gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1474
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1474
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Met Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1475
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1475
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgaa taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1476
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1476
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Asn Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1477
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1477
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgcc gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1478
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1478
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Pro Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1479
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1479
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgca gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1480
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1480
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Gln Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1481
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1481
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgag gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1482
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1482
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Arg Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1483
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1483
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgag taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1484
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1484
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ser Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1485
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1485
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgac gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1486
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1486
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Thr Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1487
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1487
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1488
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1488
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Val Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1489
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1489
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtg gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1490
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1490
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Trp Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1491
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1491
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgta caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1492
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1492
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Tyr Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1493
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1493
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgcgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1494
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1494
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Ala Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1495
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1495
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctgtcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1496
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1496
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Cys Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1497
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1497
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1498
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1498
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Asp Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1499
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1499
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1500
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1500
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Glu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1501
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1501
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctttcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1502
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1502
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Phe Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1503
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1503
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1504
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1504
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Gly Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1505
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1505
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1506
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1506
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser His Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1507
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1507
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcattcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1508
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1508
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Ile Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1509
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1509
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcaagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1510
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1510
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Lys Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1511
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1511
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcatgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1512
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1512
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Met Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1513
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1513
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcaatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1514
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1514
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Asn Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1515
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1515
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcccacgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1516
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1516
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Pro Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1517
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1517
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1518
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1518
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Gln Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1519
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1519
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1520
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1520
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Arg Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1521
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1521
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctctcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1522
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1522
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Ser Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1523
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1523
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcacgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1524
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1524
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Thr Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1525
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1525
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgtgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1526
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1526
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Val Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1527
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1527
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1528
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1528
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Trp Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1529
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1529
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1530
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1530
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Tyr Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1531
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1531
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggcta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1532
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1532
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Ala Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1533
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1533
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtgta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1534
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1534
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Cys Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1535
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1535
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1536
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1536
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Asp Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1537
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1537
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1538
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1538
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Glu Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1539
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1539
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgttta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1540
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1540
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Phe Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1541
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1541
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgggga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1542
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1542
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Gly Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1543
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1543
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1544
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1544
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu His Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1545
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1545
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgatta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1546
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1546
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Ile Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1547
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1547
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgaaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1548
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1548
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Lys Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1549
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1549
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgctga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1550
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1550
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Leu Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1551
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1551
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgatga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1552
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1552
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Met Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1553
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1553
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgaata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1554
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1554
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Asn Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1555
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1555
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgccga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1556
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1556
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Pro Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1557
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1557
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1558
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1558
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1559
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1559
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgagta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1560
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1560
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Ser Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1561
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1561
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgacga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1562
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1562
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Thr Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1563
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1563
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggtta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1564
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1564
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Val Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1565
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1565
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtgga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1566
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1566
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Trp Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1567
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1567
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1568
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1568
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Tyr Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1569
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1569
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgcgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1570
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1570
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Ala Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1571
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1571
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1572
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1572
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Cys Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1573
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1573
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1574
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1574
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Asp Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1575
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1575
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1576
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1576
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Glu Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1577
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1577
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtttcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1578
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1578
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Phe Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1579
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1579
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgggta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1580
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1580
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Gly Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1581
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1581
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1582
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1582
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly His Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1583
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1583
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtatcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1584
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1584
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Ile Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1585
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1585
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtaagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1586
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1586
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Lys Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1587
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1587
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcttta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1588
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1588
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Leu Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1589
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1589
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtatgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1590
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1590
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Met Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1591
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1591
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtaacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1592
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1592
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Asn Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1593
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1593
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtccgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1594
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1594
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Pro Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1595
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1595
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1596
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1596
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Gln Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1597
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1597
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtagcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1598
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1598
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Ser Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1599
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1599
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtacgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1600
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1600
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Thr Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1601
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1601
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgtgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1602
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1602
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Val Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1603
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1603
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttggta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1604
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1604
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Trp Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1605
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1605
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1606
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1606
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Tyr Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1607
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1607
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1608
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1608
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1609
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1609
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatgtc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1610
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1610
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Cys Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1611
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1611
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1612
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1612
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Asp Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1613
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1613
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1614
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1614
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Glu Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1615
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1615
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatttc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1616
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1616
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Phe Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1617
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1617
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaggtc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1618
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1618
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Gly Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1619
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1619
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1620
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1620
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro His Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1621
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1621
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaattc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1622
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1622
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ile Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1623
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1623
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1624
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1624
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Lys Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1625
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1625
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccattgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1626
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1626
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Leu Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1627
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1627
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaatgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1628
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1628
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Met Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1629
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1629
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1630
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1630
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Asn Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1631
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1631
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1632
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1632
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Gln Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1633
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1633
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaggc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1634
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1634
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Arg Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1635
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1635
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatcgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1636
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1636
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ser Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1637
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1637
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaacgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1638
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1638
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Thr Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1639
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1639
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagttc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1640
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1640
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Val Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1641
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1641
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatggc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1642
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1642
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Trp Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1643
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1643
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1644
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1644
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Tyr Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1645
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1645
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg cgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1646
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1646
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ala Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1647
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1647
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact gtggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1648
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1648
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Cys Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1649
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1649
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1650
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1650
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1651
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1651
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1652
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1652
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Glu Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1653
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1653
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact ttggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1654
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1654
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Phe Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1655
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1655
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1656
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1656
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Gly Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1657
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1657
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1658
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1658
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr His Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1659
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1659
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ttggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1660
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1660
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ile Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1661
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1661
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1662
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1662
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Lys Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1663
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1663
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1664
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1664
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Leu Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1665
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1665
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1666
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1666
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Met Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1667
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1667
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1668
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1668
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asn Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1669
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1669
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1670
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1670
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Pro Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1671
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1671
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1672
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1672
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Gln Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1673
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1673
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1674
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1674
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Arg Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1675
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1675
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact cgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1676
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1676
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ser Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1677
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1677
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1678
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1678
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Val Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1679
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1679
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1680
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1680
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Trp Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1681
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1681
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1682
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1682
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Tyr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1683
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1683
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg cttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1684
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1684
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ala Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1685
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1685
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1686
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1686
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Cys Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1687
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1687
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1688
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1688
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Asp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1689
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1689
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1690
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1690
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Glu Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1691
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1691
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1692
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1692
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Phe Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1693
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1693
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1694
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1694
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Gly Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1695
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1695
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1696
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1696
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ile Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1697
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1697
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1698
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1698
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Lys Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1699
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1699
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1700
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1700
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Leu Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1701
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1701
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1702
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1702
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Met Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1703
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1703
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1704
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1704
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Asn Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1705
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1705
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1706
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1706
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Pro Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1707
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1707
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1708
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1708
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Gln Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1709
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1709
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc gttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1710
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1710
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Arg Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1711
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1711
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1712
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1712
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1713
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1713
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1714
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1714
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Thr Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1715
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1715
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1716
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1716
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1717
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1717
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1718
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1718
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1719
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1719
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1720
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1720
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn Tyr Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1721
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1721
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgcgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1722
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1722
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Ala Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1723
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1723
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttgtt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1724
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1724
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Cys Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1725
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1725
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1726
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1726
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Asp Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1727
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1727
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgagt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1728
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1728
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Glu Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1729
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1729
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1730
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1730
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Gly Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1731
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1731
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtcatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1732
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1732
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly His Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1733
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1733
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtattt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1734
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1734
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Ile Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1735
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1735
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaagt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1736
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1736
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Lys Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1737
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1737
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtttgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1738
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1738
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Leu Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1739
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1739
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtatgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1740
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1740
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Met Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1741
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1741
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1742
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1742
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Asn Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1743
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1743
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtccgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1744
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1744
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Pro Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1745
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1745
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtcaat acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1746
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1746
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Gln Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1747
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1747
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1748
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1748
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Arg Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1749
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1749
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttctt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1750
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1750
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Ser Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1751
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1751
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtactt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1752
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1752
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Thr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1753
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1753
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1754
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1754
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Val Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1755
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1755
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1756
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1756
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Trp Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1757
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1757
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa 1158
<210> 1758
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1758
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Pro Lys
385
<210> 1759
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1759
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggcgaaa 1158
<210> 1760
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1760
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Ala Lys
385
<210> 1761
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1761
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtgtaaa 1158
<210> 1762
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1762
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Cys Lys
385
<210> 1763
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1763
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggataaa 1158
<210> 1764
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1764
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Asp Lys
385
<210> 1765
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1765
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggagaaa 1158
<210> 1766
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1766
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Glu Lys
385
<210> 1767
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1767
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa 1158
<210> 1768
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1768
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Phe Lys
385
<210> 1769
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1769
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgggtaaa 1158
<210> 1770
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1770
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Gly Lys
385
<210> 1771
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1771
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcataaa 1158
<210> 1772
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1772
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
His Lys
385
<210> 1773
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1773
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgattaaa 1158
<210> 1774
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1774
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Ile Lys
385
<210> 1775
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1775
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgaagaaa 1158
<210> 1776
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1776
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Lys Lys
385
<210> 1777
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1777
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttgaaa 1158
<210> 1778
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1778
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Leu Lys
385
<210> 1779
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1779
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgatgaaa 1158
<210> 1780
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1780
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Met Lys
385
<210> 1781
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1781
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgaataaa 1158
<210> 1782
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1782
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Asn Lys
385
<210> 1783
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1783
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcagaaa 1158
<210> 1784
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1784
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Gln Lys
385
<210> 1785
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1785
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcgtaaa 1158
<210> 1786
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1786
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Arg Lys
385
<210> 1787
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1787
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtcgaaa 1158
<210> 1788
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1788
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Ser Lys
385
<210> 1789
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1789
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgacgaaa 1158
<210> 1790
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1790
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Thr Lys
385
<210> 1791
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1791
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggtgaaa 1158
<210> 1792
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1792
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Val Lys
385
<210> 1793
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1793
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtggaaa 1158
<210> 1794
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1794
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Trp Lys
385
<210> 1795
<211> 1158
<212> DNA
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1795
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtataaa 1158
<210> 1796
<211> 386
<212> PRT
<213> Artificial Sequence
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
<400> 1796
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu
1 5 10 15
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly
20 25 30
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr
35 40 45
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu
50 55 60
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His
65 70 75 80
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile
85 90 95
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser
100 105 110
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly
115 120 125
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile
130 135 140
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser
145 150 155 160
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile
165 170 175
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly
180 185 190
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly
195 200 205
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val
210 215 220
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu
225 230 235 240
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro
245 250 255
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val
260 265 270
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr
275 280 285
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro
290 295 300
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val
305 310 315 320
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala
325 330 335
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp
340 345 350
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe
355 360 365
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu
370 375 380
Tyr Lys
385
Claims (47)
- 서열번호 2에 대해 적어도 70%, 적어도 75%, 적어도 80%, 적어도 85%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 또는 적어도 99% 서열 동일성을 포함하는 아미노산 서열을 포함하는 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 94, 350, 442, 540, 646, 758, 및/또는 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 2, 3, 4, 22, 27/46/97/136/149/385, 27/46/385, 27/70/136/231/385, 27/136/323/385, 27/149, 27/149/385, 27/385, 34, 38, 41, 44, 46/70, 46/149/183/231/385, 48, 70, 73, 73/305, 82, 82/94/101/194/199, 82/94/199, 83, 85, 87, 89, 92, 94, 96, 97/149/385, 135, 136/385, 140, 141, 142, 144, 146, 149, 149/231/385, 151, 174, 175, 178, 181, 183/385, 189, 194, 194/199, 195, 195/231/385, 199, 199/213, 210, 212, 213, 213/330, 216, 218, 219, 226, 231/385, 238, 247, 250, 270, 274, 281, 292, 296, 300, 308, 330, 338, 379, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 2를 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 94에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 3/4/96/296/300, 3/292/296, 4, 4/11/149/292/296/300, 4/96/149/231/296/300, 4/96/149/292/296/300, 4/96/219/296, 4/96/231/296/300, 4/96/292/296/300, 4/149/174, 4/174/219/292/296, 4/231/296/300, 27, 27/34/82, 27/73/82/218, 27/89, 27/89/178, 27/89/218, 27/218, 34/73/218, 34/82, 34/218, 73/82, 73/82/183, 94/146/175, 96/149/174/292/296, 96/149/231/292/296, 96/149/292/296, 96/174/219/231/292/296, 96/231/296, 146/175/189/281, 149/174/292/296, 149/174/300, 149/219/231/292/296/300, 149/231/292/296, 149/231/292/296/300, 149/296, 149/296/300, 174/231, 174/296, 218, 231, 231/292/296, 231/292/296/300, 231/296, 231/300, 292/296, 292/296/300, 296, 및 296/300으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 94를 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 350에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 4, 4/27/189/300, 4/70, 4/102, 4/137, 4/175/189/218, 4/175/189/300, 4/175/218/224, 4/175/218/373, 4/185, 4/189/218/373, 4/193, 4/218/300/369/373, 4/228, 4/233, 4/243, 4/271, 4/303, 4/334, 4/336, 4/375, 25, 27/82/174/175/189/218/300/369/373, 27/82/174/175/218/300/369, 27/82/174/175/218/300/373/382, 27/82/174/218/300/373, 27/189/218/373, 28, 33, 99, 102, 104, 134, 153, 174/175/189, 174/373, 175/189/218/300/373, 175/189/373, 175/218, 175/218/382, 189/218/300/373, 191, 193, 231, 233, 243, 293, 303, 331, 336, 339, 368, 및 373으로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 350을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 442에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 26, 33, 33/174/193/243, 33/174/334, 33/175/218/303, 33/175/218/334/339, 33/193, 34, 38, 46, 48, 70/271/293/334, 144, 174, 174/175/193, 174/175/218/339, 174/193/303/375, 174/193/375, 174/218/233/271/293/303, 174/218/271/303, 175/193/218/233/243/375, 175/193/218/243, 175/218/375, 181, 189, 193, 193/218/243, 193/271/303/334, 193/293/303, 194, 195, 199, 210, 218, 218/243, 218/243/303/334, 218/303, 225, 238, 274, 281, 및 330으로부터 선택된 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 442를 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 540에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 34/38/174/193/195/243/281/303/330, 34/38/174/225/303, 34/38/174/303/345, 34/174, 34/174/193/195/243/281, 34/174/193/195/303/330, 34/193/195/225/243, 34/193/243/303/330, 34/281/330, 38, 38/174/193/195/243/330, 38/174/193/225/274/283/303/330, 38/174/193/303, 38/174/195/281/330, 38/174/225/243/281/330, 38/174/281, 38/174/281/303, 38/174/281/303/345, 38/193/195/225/303/345, 38/195/243/303, 38/195/281/303/330, 38/195/281/330, 38/195/303, 49, 49/51/98/252/311, 49/51/123/252/344, 49/98/120/252/344, 49/120/252/344, 49/123/252/344, 49/123/264/344, 49/123/311, 49/252/344, 49/311/344, 49/344, 51, 51/252/344, 51/344, 98, 98/344, 123/252/344, 129, 160, 161, 174/193/195/225, 174/193/303/330, 174/195/225/281/303/330/345, 174/195/243/281/345, 174/195/281/303, 174/225/243/281/303/345, 174/281/330, 174/303, 195/225/303/330, 252, 268, 및 344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 540을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 646에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 11/16/67/168/180/287, 11/16/237/241/287, 11/67/168/287, 16/67/154/168/237/241/287, 16/67/237/287/291, 16/168/177, 24, 24/278, 34/49/161/193/243/344, 34/49/161/193/344, 34/49/161/243/344, 34/49/161/252/268/344, 34/49/193/243/252/344, 34/49/193/344, 34/49/243/252, 34/49/252/268/344, 34/161/193/243/252/268, 34/161/193/243/268/344, 34/161/193/243/344, 34/161/193/252/268/344, 34/161/193/252/344, 34/161/193/268/344, 34/161/243/281/344, 34/161/344, 34/193/243/252/344, 34/193/252/268/344, 34/193/268/281, 34/252/268/281/344, 34/252/344, 49/161/193/243/252/344, 49/161/193/252/281/344, 49/161/243/252/344, 49/193/197/243/252/281/344, 49/193/243/252/344, 49/193/243/344, 49/243/344, 67/154/237/287, 67/168/237, 67/168/237/287, 67/177/237, 154/237/286/287, 154/286/287, 161/193/252/344, 161/193/344, 161/344, 168/237, 186/187/278, 193/243/281/344, 193/252/268/344, 193/252/281/344, 237, 237/287/291, 및 281/344로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 646을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 758에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 24/168/252/281, 24/237/287/344, 24/252, 24/252/287, 24/344, 213/252/278/344, 252/278/344, 252/287/344, 및 281로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 상기 폴리펩티드 서열의 아미노산 위치는 서열번호 758을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 서열번호 868에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 폴리펩티드 서열을 포함하고, 상기 폴리펩티드 서열은 24, 24/38/49/70/149/161/174/175/189/193/225/231/243/252/271/287/292/293/296/303/334/344/373/385, 38, 49, 70, 149, 161, 174, 175, 189, 193, 225, 231, 243, 252, 271, 287, 292, 293, 296, 303, 334, 344, 373, 및 385로부터 선택되는 하나 이상의 위치에서 적어도 하나의 치환 또는 치환 세트를 포함하고, 폴리펩티드 서열의 아미노산 위치는 서열번호 868을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항에 있어서, 상기 재조합 리파제는 표 4-1, 4-2, 4-3, 4-4, 4-5, 4-6, 4-7, 및/또는 5-1에서 제공되는 바와 같이 적어도 하나의 위치에서 적어도 하나의 돌연변이를 포함하고, 위치는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868을 참조하여 번호가 매겨지는 것인 재조합 리파제.
- 제1항 내지 제11항 중 어느 한 항에 있어서, 상기 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 더 열안정한 것인 재조합 리파제.
- 제1항 내지 제12항 중 어느 한 항에 있어서, 상기 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 산성 pH 수준에서 더 안정한 것인 재조합 리파제.
- 제1항 내지 제13항 중 어느 한 항에 있어서, 상기 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 단백질분해에 더 내성인 재조합 리파제.
- 제1항 내지 제14항 중 어느 한 항에 있어서, 상기 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 적어도 하나의 담즙염의 존재 하에 더 활성인 재조합 리파제.
- 제1항 내지 제15항 중 어느 한 항에 있어서, 상기 재조합 리파제는 서열번호 2, 94, 350, 442, 540, 646, 758, 및/또는 868의 리파제보다 적어도 하나의 담즙염의 존재 하에 더 활성인 재조합 리파제.
- 제1항 내지 제16항 중 어느 한 항에 있어서, 상기 재조합 리파제는 정제된 것인 재조합 리파제.
- 제1항 내지 제17항 중 어느 한 항에 있어서, 상기 재조합 리파제는 참조 서열과 비교하여 i) 향상된 촉매 활성; ii) 산성 pH에 대한 증가된 내성; iii) pH 3.5에 대한 증가된 내성; iv) pH 3에 대한 증가된 내성; v) 적어도 하나의 프로테아제에 대한 증가된 내성; vi) 적어도 하나의 담즙염에 대한 증가된 내성; vii) 증가된 내열성; 또는 i), ii), iii), iv), v), vi), 및 vii) 중 임의의 것의 조합으로부터 선택되는 적어도 하나의 개선된 특성을 나타내는 것인 재조합 리파제.
- 제18항에 있어서, 상기 참조 서열은 서열번호 2, 94, 350, 442, 540, 646, 758, 및 868로부터 선택되는 것인 재조합 리파제.
- 제1항 내지 제19항 중 어느 한 항의 적어도 하나의 재조합 리파제를 포함하는 조성물.
- 제1항 내지 제19항 중 어느 한 항에 제시된 적어도 하나의 재조합 리파제를 코딩하는 재조합 폴리뉴클레오티드 서열.
- 제21항에 있어서, 상기 폴리뉴클레오티드 서열은 코돈 최적화된 것인 재조합 폴리뉴클레오티드 서열.
- 제21항 또는 제22항의 적어도 하나의 재조합 폴리뉴클레오티드 서열을 포함하는 발현 벡터.
- 제23항에 있어서, 상기 재조합 폴리뉴클레오티드 서열은 제어 서열에 작동 가능하게 연결된 것인 발현 벡터.
- 제23항 또는 제24항에 있어서, 상기 제어 서열은 프로모터인 발현 벡터.
- 제25항에 있어서, 상기 프로모터는 이종 프로모터인 발현 벡터.
- 제23항 내지 제26항 중 어느 한 항의 발현 벡터를 포함하는 숙주 세포.
- 제27항에 있어서, 상기 숙주 세포는 진핵성 또는 원핵성인 숙주 세포.
- 제27항 또는 제28항의 상기 숙주 세포를, 상기 재조합 폴리뉴클레오티드에 의해 코딩되는 상기 재조합 리파제가 생성되는 조건 하에 배양하는 단계를 포함하는, 재조합 리파제의 제조 방법.
- 제29항에 있어서, 상기 리파제를 회수하는 단계를 추가로 포함하는 제조 방법.
- 제30항에 있어서, 상기 리파제를 정제하는 단계를 추가로 포함하는 제조 방법.
- 제20항의 조성물을 포함하는, 췌장 기능부전을 치료하기 위한 약학적 조성물.
- 제32항에 있어서, 약학적으로 허용되는 담체 및/또는 부형제를 추가로 포함하는 것인 약학적 조성물.
- 제32항 또는 제33항에 있어서, 적어도 하나의 프로테아제 및 적어도 하나의 아밀라제로부터 선택되는 적어도 하나의 추가 효소를 추가로 포함하는 약학 조성물.
- 제32항 또는 제33항에 있어서, 리파제, 프로테아제 및 아밀라제 활성을 함유하는 췌장 추출물을 추가로 포함하는 약학 조성물.
- 제32항 내지 제35항 중 어느 한 항에 있어서, 상기 조성물은 인간에 대한 비경구 주사 또는 주입, 피하 주사, 흡입, 또는 피부 적용에 적합한 것인 약학적 조성물.
- 제32항 내지 제35항 중 어느 한 항에 있어서, 상기 조성물은 인간에 대한 경구 투여에 적합한 것인 약학적 조성물.
- 제37항에 있어서, 상기 조성물은 장용 코팅을 추가로 포함하는 것인 약학적 조성물.
- 췌장 기능부전을 갖는 대상체를 제공하는 단계, 및 제32항 내지 제38항 중 어느 한 항의 약학적 조성물을 상기 대상체에게 제공하는 단계를 포함하는, 대상체에서 췌장 기능부전의 증상을 치료 및/또는 예방하는 방법.
- 제39항에 있어서, 상기 췌장 기능부전의 증상이 개선되는 것인 방법.
- 제39항 또는 제40항에 있어서, 상기 대상체는 췌장 기능부전의 증상을 나타내는 대상체가 필요로 하는 식이보다 지질 함량이 덜 제한되는 식이를 먹을 수 있는 것인 방법.
- 제39항 내지 제41항 중 어느 한 항에 있어서, 상기 대상체는 유아 또는 소아인 방법.
- 제39항 내지 제41항 중 어느 한 항에 있어서, 상기 대상체는 성인 또는 젊은 성인인 방법.
- 의약으로서 사용하기 위한, 제1항 내지 제19항 중 어느 한 항에 따른 재조합 리파제, 또는 제20항 및 제32항 내지 제38항 중 어느 한 항에 따른 조성물.
- 영양 보충제로서 사용하기 위한, 제1항 내지 제19항 중 어느 한 항에 따른 재조합 리파제, 또는 제20항 및 제32항 내지 제38항 중 어느 한 항에 따른 조성물.
- 췌장 기능부전의 증상의 치료 또는 예방에 사용하기 위한, 제1항 내지 제19항 중 어느 한 항에 따른 재조합 리파제, 또는 제20항 및 제32항 내지 제38항 중 어느 한 항에 따른 조성물.
- 제20항 및 제32항 내지 제38항 중 어느 한 항에 제공된 조성물의 용도.
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PCT/US2020/048405 WO2021041809A1 (en) | 2019-08-30 | 2020-08-28 | Engineered lipase variants |
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BR (1) | BR112022003705A2 (ko) |
CA (1) | CA3152763A1 (ko) |
IL (1) | IL290978A (ko) |
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