KR20220137057A - 조작된 류신 데카르복실라제 - Google Patents

조작된 류신 데카르복실라제 Download PDF

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KR20220137057A
KR20220137057A KR1020227030236A KR20227030236A KR20220137057A KR 20220137057 A KR20220137057 A KR 20220137057A KR 1020227030236 A KR1020227030236 A KR 1020227030236A KR 20227030236 A KR20227030236 A KR 20227030236A KR 20220137057 A KR20220137057 A KR 20220137057A
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조이스 리우
리앤 퀠틴몬트 티트
리앤 ??틴몬트 티트
니키 델라스
스테판 젠느
페이 론 두
크리스틴 진 밸리우
커린 맥클루스키
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract

본 발명은 조작된 류신 데카르복실라제(LDC) 폴리펩타이드 및 그의 조성물, 및 조작된 류신 데카복실라제(LDC) 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 향상된 촉매 활성뿐만 아니라, 단백질 분해에 대한 감소된 민감성, 및/또는 낮은 pH 환경에 대한 증가된 내성을 제공하도록 최적화된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 개선된 저장 안정성을 제공하도록 최적화된다. 본 발명은 또한 치료 및 산업적 목적을 위한, 조작된 LDC 폴리펩타이드를 포함하는 조성물의 사용 방법을 제공한다.

Description

조작된 류신 데카르복실라제
본 출원은 모든 목적을 위해 그 전체가 본 명세서에 참고로 포함된, 2020년 2월 4일에 출원된 미국 특허 가출원 제62/970,039호에 기초한 우선권을 주장한다.
발명의 분야
본 발명은 조작된 류신 데카르복실라제(LDC) 폴리펩타이드 및 그의 조성물, 및 조작된 류신 데카복실라제(LDC) 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 향상된 촉매 활성뿐만 아니라, 단백질 분해에 대한 감소된 민감성, 및/또는 낮은 pH 환경에 대한 증가된 내성을 제공하도록 최적화된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 개선된 저장 안정성을 제공하도록 최적화된다. 본 발명은 또한 치료 및 산업적 목적을 위한, 조작된 LDC 폴리펩타이드를 포함하는 조성물의 사용 방법을 제공한다.
ASCII 텍스트 파일로 제출된 "서열 목록", 표 또는 컴퓨터 프로그램 목록 부록에 대한 언급
머신 포맷 IBM-PC, MS-윈도우즈(Windows) 운영 체제를 사용하여 2021년 2월 3일에 생성된 2.06 메가바이트 크기의 파일 CX7-198WO2_ST25.TXT로서 작성된 서열 목록은 본 명세서에 참조로 포함된다.
"류신뇨증", 분지쇄 알파-류신 데히드로게나제 결핍증, 및 "BCKD 결핍증"으로도 지칭되는 메이플 시럽 요증(MSUD: maple syrup urine disease)은 류신, 이소류신 및 발린(즉, 분지쇄 아미노산)의 이화 경로에 관여하는 분지쇄 케토산 데히드로게나제(BCKDH) 복합체의 기능 장애에 이차적으로 발생하는 드문 유전성 아미노산병증이다. 이것은 멘케스 등에 의해 1954년도에 처음 제시되었고(Menkes et al., Pediatrics 14:462-467 [1954]), 이환된 신생아의 소변에서 나는 특유한 달콤한 냄새 때문에 그와 같이 명명되었다. 또한, 이것은 식욕 부진, 구토, 무기력, 비정상적인 움직임(예를 들어, 과긴장 또는 저긴장), 발달 지연을 특징으로 한다. 치료하지 않으면, 질병은 뇌병증, 발작, 혼수 상태, 영구적인 신경 손상 및 사망으로 진행될 수 있다. 추후에, 발달 지연, 학습 문제, 발작 및 운동 장애가 일반적이다. 질병의 징후 및 증상에 따라 분류되는 4가지의 일반적인 형태가 있다. 가장 흔하고 심각한 유형은 출생 후 2주 이내에 명백해지는 "전통적인" 유형이다. 다른 유형은 중간형 MSUD, 간헐형 MSUD 및 티아민 반응형 MSUD이다. 전통적인 형태의 경우, 신생아가 단백질을 함유한 우유를 섭취한 후에 질병이 명백해진다. 이로 인해 체내에서 이소류신, 류신 및 발린이 증가하여 뇌에 독성이 생긴다. 간헐형 형태에서는, 물리적 스트레스(예를 들어, 감염, 발열, 장기간 금식) 동인 뇌 손상이 발생하여, 대사 대상부전(metabolic decompensation)으로 이어진다.
신생아에서 MSUD에 대한 진단 테스트는 혈액 및 소변 내의 류신, 이소류신, 알로이소류신 및 발린의 농도를 결정하기 위한 혈액 및 소변 아미노산 테스트를 포함한다. MSUD가 확인되면, 케톤증 및 산증의 징후가 나타날 것이다. 진단시 및 증상이 있는 기간 동안에, 치료에는 단백질이 없는 식단을 섭취하고 상승된 아미노산 수준과 관련된 대사 결과를 교정하는 것이 포함된다. 특별한 정맥내 용액을 사용하면, 류신 수치(가장 독성이 강함)가 감소하고, 에너지 결핍이 교정된다.
현재의 치료는 분지쇄 아미노산(BCAA)의 식이 제한을 포함한다. BCKDH 복합 효소의 수준 결핍은 뇌척수액, 혈액 및 조직에서 BCAA 및 그의 관련 대사산물의 독성 축적을 초래한다. 치료 또는 지속적인 주의를 기울이지 않으면, 수많은 심각한 부작용(예를 들어, 신경 기능 장애, 발작 및 영아 사망)이 발생한다. 신장 청소를 통한 일부 BCAA 배출(이환된 환자의 소변에서 전형적인 달콤한 메이플 시럽 냄새가 남)이 발생하기는 하지만, 체내 독성 아미노산 수준의 축적을 완화시키기에는 충분하지 않다(문헌 [Schadewalt and Wendel, Eur. J. Pediatr., 156(Suppl. 1): S62-66 [1997]]; 및 [Skvorak, J. Inherit. Metab. Dis., 32(2):229-46 [2009]] 참조).
발명의 요약
본 발명은 조작된 류신 데카르복실라제(LDC) 폴리펩타이드 및 그의 조성물, 및 조작된 류신 데카복실라제(LDC) 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 향상된 촉매 활성뿐만 아니라, 단백질 분해에 대한 감소된 민감성, 및/또는 낮은 pH 환경에 대한 증가된 내성을 제공하도록 최적화된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 개선된 저장 안정성을 제공하도록 최적화된다. 본 발명은 또한 치료 및 산업적 목적을 위한, 조작된 LDC 폴리펩타이드를 포함하는 조성물의 사용 방법을 제공한다.
본 발명은 본질적으로 동일한 조건 하에 야생형 LDC 효소 또는 참조 LDC 폴리펩타이드와 비교할 때 개선된 특성을 갖는 조작된 LDC 폴리펩타이드 및 생물학적 활성 단편 및 그의 유사체에 관한 것이다. 본 발명은 추가로 치료 및/또는 산업적 조성물에서 조작된 LDC 폴리펩타이드 및 그의 생물학적 활성 단편 및 유사체를 사용하는 방법 및 치료 및/또는 산업적 목적을 위해 이러한 조성물을 사용하는 방법에 관한 것이다.
본 발명은 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766 중 적어도 하나에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 아미노산 서열을 포함하는 조작된 류신 데카르복실라제 폴리펩타이드를 제공하고, 여기서 상기 아미노산 서열의 아미노산 위치는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766의 아미노산 서열을 기준으로 하여 넘버링된다.
본 발명은 또한 폴리펩타이드 서열이 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 제공한다. 본 발명은 폴리펩타이드 서열이 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 추가로 제공한다. 본 발명은 폴리펩타이드 서열이 서열 번호 6에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 추가로 제공한다. 본 발명은 폴리펩타이드 서열이 서열 번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 추가로 제공한다. 본 발명은 폴리펩타이드 서열이 서열 번호 10에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 추가로 제공한다. 본 발명은 폴리펩타이드 서열이 서열 번호 14에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖는 조작된 류신 데카르복실라제 폴리펩타이드를 추가로 제공한다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 12에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00001
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00002
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00003
Figure pct00004
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 38에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00005
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00006
Figure pct00007
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00008
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 234에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00009
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00010
Figure pct00011
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00012
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 284에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00013
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00014
Figure pct00015
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00016
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 484에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00017
Figure pct00018
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00019
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00020
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 594에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00021
으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00022
으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00023
으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 686에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00024
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00025
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00026
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드 서열은 서열 번호 686에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성을 갖고, 여기서 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 19, 109, 123, 134, 170, 173, 187, 211, 및 312로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 19I, 109G, 123F, 123M, 123V, 134A, 134S, 170A, 173A, 173I, 173T, 187L, 211S, 및 312A로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 L19I, L109G, Y123F, Y123M, Y123V, N134A, N134S, P170A, F173A, F173I, F173T, V187L, A211S, 및 T312A로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00027
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00028
Figure pct00029
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00030
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00031
Figure pct00032
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00033
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00034
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00035
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00036
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00037
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00038
Figure pct00039
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00040
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00041
Figure pct00042
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00043
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00044
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00045
Figure pct00046
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00047
으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00048
으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00049
Figure pct00050
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00051
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00052
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00053
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 19, 109, 123, 134, 170, 173, 187, 211, 및 312로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 19I, 109G, 123F, 123M, 123V, 134A, 134S, 170A, 173A, 173I, 173T, 187L, 211S, 및 312A로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 L19I, L109G, Y123F, Y123M, Y123V, N134A, N134S, P170A, F173A, F173I, F173T, V187L, A211S, 및 T312A로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00054
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 688을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00055
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 688을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은
Figure pct00056
로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 688을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 5/41, 5/41/228, 33, 41, 47, 51, 55, 64, 126, 265, 267, 270, 331, 353, 357, 및 384로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 5V/41D, 5V/41D/228D, 33L, 41D, 47F, 51E, 51Q, 55I, 64N, 126A, 126T, 265P, 267L, 270A, 270T, 331V, 353E, 353I, 353L, 357S, 및 384W로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 K5V/H41D, K5V/H41D/T228D, F33L, H41D, L47F, L51E, L51Q, V55I, S64N, D126A, D126T, E265P, I267L, R270A, R270T, T331V, D353E, D353I, D353L, C357S, 및 P384W로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 66, 66/118, 66/118/296, 66/118/296/300, 66/118/300, 66/296, 66/296/300, 66/300, 118, 118/296, 118/296/300, 118/300, 296, 296/300, 및 300으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 66S, 66S/118T, 66S/118T/296D, 66S/118T/296D/300R, 66S/118T/300R, 66S/296D, 66S/296D/300R, 66S/300R, 118T, 118T/296D, 118T/296D/300R, 118T/300R, 296D, 296D/300R, 및 300R로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다. 추가의 일부 실시양태에서, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열은 N66S, N66S/D118T, N66S/D118T/E296D, N66S/D118T/E296D/K300R, N66S/D118T/K300R, N66S/E296D, N66S/E296D/K300R, N66S/K300R, D118T, D118T/E296D, D118T/E296D/K300R, D118T/K300R, E296D, E296D/K300R, 및 K300R로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링된다.
추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 아미노산 서열, 또는 그의 기능적 단편을 포함한다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766에 대해 적어도 90%의 서열 동일성을 갖는 아미노산 서열, 또는 그의 기능적 단편을 포함한다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766에 대해 적어도 95%의 서열 동일성을 갖는 아미노산 서열, 또는 그의 기능적 단편을 포함한다. 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1, 및/또는 11-2 중 어느 하나에 제시된 변이체 류신 데카르복실라제 폴리펩타이드이다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 플랑크토마이세타세아에(Planctomycetaceae) 박테리아 종 변이체 효소이다. 일부 실시양태에서, 류신 데카르복실라제는 야생형 플랑크토마이세타세아에 박테리아 종 류신 데카르복실라제와 비교하여 적어도 하나의 개선된 특성을 나타낸다. 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 야생형 플랑크토마이세타세아에 종 류신 데카르복실라제보다 류신에 대해 더 많은 활성을 나타낸다. 또 다른 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 야생형 플랑크토마이세타세아에 박테리아 종 류신 데카르복실라제보다 열 안정성이 더 높다. 또 다른 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 야생형 플랑크토마이세타세아에 박테리아 종 류신 데카르복실라제보다 단백질 분해에 대해 내성이 더 높다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 야생형 플랑크토마이세타세아에 박테리아 종 류신 데카르복실라제보다 면역원성이 더 낮다. 또 다른 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 야생형 플랑크토마이세타세아에 박테리아 종 류신 데카르복실라제보다 혈청 안정성이 더 높다. 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 16-852의 임의의 짝수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과로 동일한 서열을 포함한다. 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 16-852의 임의의 짝수 서열에 대해 적어도 90% 동일한 서열을 포함한다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 서열 번호 16-852의 임의의 짝수 서열을 포함한다. 추가의 일부 실시양태에서, 조작된 류신 데카르복실라제 폴리펩타이드는 정제된다. 본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 조성물을 제공한다. 본 발명은 또한 본원에서 제공되는 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 조성물을 제공한다.
본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드 서열을 제공한다. 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 본원에서 제공되는 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩한다. 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 서열 번호 15-851의 임의의 홀수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과로 동일한 서열을 포함한다. 추가의 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 서열 번호 15-851의 임의의 홀수 서열에 대해 적어도 90% 또는 그 초과로 동일한 서열을 포함한다. 추가의 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 서열 번호 15-851의 임의의 홀수 서열을 포함한다. 추가의 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 제어 서열에 작동 가능하게 연결된다. 일부 실시양태에서, 조작된 폴리뉴클레오타이드 서열은 코돈 최적화된다.
본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 폴리뉴클레오타이드 서열을 포함하는 발현 벡터를 제공한다. 일부 실시양태에서, 발현 벡터는 적어도 하나의 제어 서열을 추가로 포함한다. 일부 실시양태에서, 제어 서열은 프로모터를 포함한다. 추가의 일부 실시양태에서, 프로모터는 이종 프로모터이다.
본 발명은 또한 적어도 하나의 폴리뉴클레오타이드 서열로 형질전환되고/되거나, 본원에서 제공되는 발현 벡터를 포함하는 숙주 세포를 제공한다. 일부 실시양태에서, 숙주 세포는 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열로 형질전환된다. 일부 실시양태에서, 숙주 세포는 본원에서 제공되는 하나의 폴리뉴클레오타이드 서열로 형질전환된다. 추가의 일부 실시양태에서, 숙주 세포는 본원에서 제공되는 적어도 하나의 발현 벡터를 포함한다. 추가의 일부 실시양태에서, 숙주 세포는 본원에서 제공되는 하나의 발현 벡터를 포함한다. 일부 실시양태에서, 숙주 세포는 이. 콜라이(E. coli)이다.
본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드, 및/또는 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열 및/또는 본원에서 제공되는 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함하는, 숙주 세포에서 조작된 류신 데카르복실라제 폴리펩타이드를 생산하는 방법을 제공한다. 일부 실시양태에서, 숙주 세포에서 조작된 류신 데카르복실라제 폴리펩타이드를 생산하는 방법은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함한다. 추가의 일부 실시양태에서, 숙주 세포에서 조작된 류신 데카르복실라제 폴리펩타이드를 생산하는 방법은 본원에서 제공되는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함한다. 일부 실시양태에서, 숙주 세포에서 조작된 류신 데카르복실라제 폴리펩타이드를 생산하는 방법은 본원에서 제공되는 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함한다. 일부 실시양태에서, 상기 방법은 배양물 및/또는 숙주 세포로부터 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 회수하는 단계를 추가로 포함한다. 추가의 일부 실시양태에서, 상기 방법은 상기 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 정제하는 단계를 추가로 포함한다.
본 발명은 또한 본원에서 제공되는 적어도 하나의 조작된 폴리뉴클레오타이드를 포함하는 조성물을 제공한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 조작된 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 약학 조성물이다. 추가의 일부 실시양태에서, 조성물은 적어도 하나의 약제학상 허용되는 부형제 및/또는 담체를 추가로 포함한다. 일부 실시양태에서, 조성물은 메이플 시럽 요증의 치료에 적합하다. 추가의 일부 실시양태에서, 조성물은 유전자 요법에 사용하기에 적합하다. 또 다른 일부의 추가의 실시양태에서, 조성물은 메이플 시럽 요증, 및/또는 이소류신, 류신, 알로이소류신 및/또는 발린의 상승된 혈액 수준을 치료하기 위한 유전자 요법에 사용하기에 적합하다. 추가의 일부 실시양태에서, 조성물은 mRNA 요법에 사용하기에 적합하다. 또 다른 추가의 일부 실시양태에서, 조성물은 인간에 대한 경구 투여에 적합하다. 일부 실시양태에서, 조성물은 환제, 정제, 캡슐, 젤캡(gelcap), 액체 또는 에멀젼의 형태이다. 추가의 일부 실시양태에서, 환제, 정제, 캡슐 또는 젤캡은 장용 코팅을 추가로 포함한다. 또 다른 추가의 일부 실시양태에서, 조성물은 동물에 대한 비경구 주사에 적합하다. 또 다른 추가의 일부 실시양태에서, 조성물은 인간에 대한 비경구 주사에 적합하다. 일부 실시양태에서, 주사는 매일, 매주, 또는 매월 기준으로 투여된다. 추가의 일부 실시양태에서, 조성물은 적어도 하나의 추가의 치료상 효과적인 화합물과 공동으로 투여된다. 일부 실시양태에서, 조성물은 적어도 하나의 추가의 치료상 효과적인 화합물을 포함한다.
본 발명은 또한 메이플 시럽 요증이 있는 대상체를 제공하는 단계, 및 본원에서 제공되는 조성물을 상기 대상체에게 제공하는 단계를 포함하는, 대상체에서 메이플 시럽 요증의 증상을 치료 및/또는 예방하는 방법을 제공한다. 일부 실시양태에서, 메이플 시럽 요증의 증상이 개선된다. 추가의 일부 실시양태에서, 대상체는 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드 및/또는 폴리뉴클레오타이드를 포함하는 적어도 하나의 조성물이 제공되지 않은 대상체에 의해 요구되는 식이보다 이소류신, 류신 및/또는 발린 함량이 덜 제한된 식이를 먹을 수 있다. 일부 실시양태에서, 대상체는 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 적어도 하나의 조성물이 제공되지 않은 대상체에 의해 요구되는 식이보다 이소류신, 류신 및/또는 발린 함량이 덜 제한된 식이를 먹을 수 있다.
일부 실시양태에서, 대상체는 유아, 아동, 청소년, 또는 성인이다. 일부 실시양태에서, 대상체는 유아이다. 일부 실시양태에서, 대상체는 아동이다.
일부 실시양태에서, 대상체는 청소년이다. 일부 실시양태에서, 대상체는 성인이다. 본 발명은 또한 본원에서 제공되는 조성물의 용도를 제공한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드 및/또는 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 조작된 류신 데카르복실라제 폴리펩타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 조작된 류신 데카르복실라제 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 2개의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 2개의 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 하나의 조작된 류신 데카르복실라제를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 적어도 2개의 조작된 류신 데카르복실라제를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함한다. 일부 실시양태에서, 조성물은 본원에서 제공되는 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함한다.
발명의 설명
본 발명은 조작된 류신 데카르복실라제(LDC) 폴리펩타이드 및 그의 조성물, 및 조작된 류신 데카복실라제(LDC) 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 향상된 촉매 활성뿐만 아니라, 단백질 분해에 대한 감소된 민감성, 및/또는 낮은 pH 환경에 대한 증가된 내성을 제공하도록 최적화된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 개선된 저장 안정성을 제공하도록 최적화된다. 본 발명은 또한 치료 및 산업적 목적을 위한, 조작된 LDC 폴리펩타이드를 포함하는 조성물의 사용 방법을 제공한다. 본 발명은 조작된 LDC 폴리펩타이드, 그의 돌연변이체, 생물학적 활성 단편 및 유사체, 및 이를 포함하는 약제학적 및 산업적 조성물을 제공한다.
약어 및 정의:
달리 정의되지 않는 한, 본 명세서에서 사용되는 모든 기술 및 과학 용어는 일반적으로 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 일반적으로 이해하는 것과 동일한 의미를 갖는다. 일반적으로, 본 명세서에 사용된 명명법 및 아래에서 설명되는 세포 배양, 분자 유전학, 미생물학, 유기 화학, 분석 화학 및 핵산 화학의 실험실 절차는 관련 기술 분야에 널리 공지되고 일반적으로 사용되는 것이다. 이러한 기술은 잘 알려져 있고, 관련 기술 분야의 통상의 기술자에게 잘 알려진 많은 텍스트 및 참고 문헌에 설명되어 있다. 화학 합성 및 화학 분석에는 표준 기술 또는 그 변형이 사용된다. 상기 및 하기에서 언급된 모든 특허, 특허 출원, 기사 및 간행물은 본 명세서에 참조로 명시적으로 포함된다.
본 명세서에 기재된 것과 유사하거나 동등물인 임의의 적합한 방법 및 재료가 본 발명의 실시에 사용되지만, 일부 방법 및 재료가 본 명세서에 기재되어 있다. 본 발명은 기술된 특정 방법, 프로토콜 및 시약으로 제한되지 않는 것으로 이해되어야 하는데, 이는 이들이 관련 기술 분야의 통상의 기술자에 의해 사용되는 맥락에 따라 달라질 수 있기 때문이다. 따라서, 바로 아래에서 정의되는 용어들은 전체로서 본 출원을 참조하여 보다 완전하게 설명된다. 상기 및 하기에서 언급된 모든 특허, 특허 출원, 기사 및 간행물은 본 명세서에 참조로 명시적으로 포함된다.
또한, 본 명세서에서 사용되는 단수 "a", "an" 및 "the"는 문맥상 명백하게 달리 지시하지 않는 한, 복수의 참조 대상을 포함한다.
수치 범위는 범위를 정의하는 수치를 포함한다. 따라서, 본 명세서에 개시된 모든 수치 범위는 마치 보다 좁은 수치 범위가 모두 본 명세서에 명시적으로 기재된 것처럼 보다 넓은 수치 범위 내에 속하는 모든 보다 좁은 수치 범위를 포괄하는 것으로 의도된다. 또한, 본 명세서에 개시되는 모든 최대(또는 최소) 수치 제한은 마치 그러한 더 낮은(또는 더 높은) 수치 제한이 본 명세서에 명시적으로 기재된 것처럼 모든 더 낮은(또는 더 높은) 수치 제한을 포함하는 것으로 의도된다.
용어 "약"은 특정 값에 대해 허용 가능한 오차를 의미한다. 일부 경우에, "약"은 주어진 값 범위의 0.05%, 0.5%, 1.0% 또는 2.0% 이내를 의미한다. 일부 경우에, "약"은 주어진 값의 1, 2, 3 또는 4 표준 편차 이내를 의미한다.
또한, 본 명세서에서 제공되는 표제는 전체로서 본 출원을 참조하여 제시될 수 있는 본 발명의 다양한 측면 또는 실시양태를 제한하는 것이 아니다. 따라서, 바로 아래에서 정의되는 용어들은 전체로서 본 출원을 참조하여 보다 완전하게 정의된다. 그럼에도 불구하고, 본 발명의 이해를 용이하게 하기 위해, 다수의 용어가 아래에 정의된다.
달리 표시되지 않는 한, 핵산은 5'에서 3' 방향으로 왼쪽에서 오른쪽으로 기록되고, 아미노산 서열은 각각 아미노에서 카르복시 방향으로 왼쪽에서 오른쪽으로 쓰여진다.
본 명세서에 사용된 바와 같이, 용어 "포함하는" 및 그 동족어는 포괄적인 의미로 사용된다(즉, 용어 "포괄하는" 및 그의 대응하는 동족어와 동등함).
"EC" 번호는 국제 생화학 및 분자 생물학 연합의 명명 위원회(NC-IUBMB: Nomenclature Committee of the International Union of Biochemistry and Molecular Biology)의 효소 명명법(Enzyme Nomenclature)을 나타낸다. IUBMB 생화학적 분류는 효소가 촉매하는 화학 반응을 기반으로 하는, 효소의 수치적 분류 시스템이다.
"ATCC"는 생물 저장소 컬렉션이 유전자 및 균주를 포함하는 아메리칸 타입 컬쳐 컬렉션(American Type Culture Collection)을 지칭한다.
"NCBI"는 미국 국립 생물 정보 센터(National Center for Biological Information) 및 본 명세서에서 제공되는 서열 데이터베이스를 의미한다.
본원에서 사용되는 바와 같이, 용어 "류신 데카르복실라제(LDC) 폴리펩타이드"는 발린 데카르복실라제 효소 클래스(EC 4.1.1.14)의 구성원을 지칭한다. 이 효소는 발린 및 류신과 같은 아미노산을 탈카르복실화하여, 이산화탄소를 방출하면서 각각 2-메틸프로판아민 및 이소펜틸아민을 생성하기 위해 피리독시알 5'-포스페이트(PLP) 보조인자를 사용한다.
"단백질", "폴리펩타이드" 및 "펩타이드"는 길이 또는 번역후 변형(예를 들어, 글리코실화 또는 인산화)에 관계없이 아미드 결합에 의해 공유 연결된 적어도 2개의 아미노산의 중합체를 나타내기 위해 본원에서 교환 가능하게 사용된다.
"폴리뉴클레오타이드"는 뉴클레오타이드가 데옥시리보뉴클레오타이드 또는 리보뉴클레오타이드인 적어도 2개의 뉴클레오타이드를 포함하는 중합체를 나타내기 위해 본원에서 사용된다.
"아미노산"은 본 명세서에서 일반적으로 알려진 3문자 기호 또는 IUPAC-IUB 생화학 명명 위원회에서 권장하는 1문자 기호로 지칭된다. 마찬가지로, 뉴클레오타이드는 표시된 바와 같이 일반적으로 허용되는 단일 문자 코드로 나타낼 수 있다. 유전자에 의해 코딩되는 아미노산에 사용되는 약어는 통상적이며 다음과 같다: 알라닌(Ala 또는 A), 아르기닌(Arg 또는 R), 아스파라긴(Asn 또는 N), 아스파르테이트(Asp 또는 D), 시스테인(Cys 또는 C), 글루타메이트(Glu 또는 E), 글루타민(Gln 또는 Q), 히스티딘(His 또는 H), 이소류신(Ile 또는 I), 류신(Leu 또는 L), 라이신(Lys 또는 K), 메티오닌(Met 또는 M), 페닐알라닌(Phe 또는 F), 프롤린(Pro 또는 P), 세린(Ser 또는 S), 트레오닌(Thr 또는 T), 트립토판(Trp 또는 W), 티로신(Tyr 또는 Y) 및 발린(Val 또는 V).
세포, 폴리뉴클레오타이드 또는 폴리펩타이드와 관련하여 사용될 때 용어 "조작된", "재조합", "비-자연 발생" 및 "변이체"는 자연에 존재하지 않거나 또는 그와 동일하지만 합성 물질로부터 및/또는 재조합 기술을 사용한 조작에 의해 생산되거나 유도되는 방식으로 변형된 물질의 자연 또는 천연 형태에 상응하는 물질을 지칭한다.
본원에서 사용되는 바와 같이, "야생형" 및 "자연 발생"은 자연에서 발견되는 형태를 지칭한다. 예를 들어, 야생형 폴리펩타이드 또는 폴리뉴클레오타이드 서열은 자연에서 공급원으로부터 단리될 수 있고 인간의 조작에 의해 의도적으로 변형되지 않은 유기체에 존재하는 서열이다.
"코딩 서열"은 단백질의 아미노산 서열을 코딩하는 핵산(예를 들어, 유전자)의 부분을 지칭한다.
용어 "서열 동일성 백분율(%)"은 폴리뉴클레오타이드 및 폴리펩타이드 사이의 비교를 지칭하기 위해 본원에 사용되며, 비교 창에서 2개의 최적으로 정렬된 서열을 비교함으로써 결정되며, 여기서 비교 창 내의 폴리뉴클레오타이드 또는 폴리펩타이드 서열의 일부는 두 서열의 최적 정렬을 위해 참조 서열과 비교하여 부가 또는 결실(즉, 갭)을 포함한다. 백분율은 일치하는 위치의 수를 산출하기 위해 두 서열에서 동일한 핵산 염기 또는 아미노산 잔기가 발생하는 위치의 수를 결정하고, 일치된 위치의 수를 비교 창 내의 총 위치 수로 나누고, 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출함으로써 계산할 수 있다. 대안적으로, 백분율은 동일한 핵산 염기 또는 아미노산 잔기가 두 서열 모두에서 발생하거나 또는 핵산 염기 또는 아미노산 잔기가 일치하는 위치의 수를 산출하기 위해 갭을 두고 정렬되는 위치의 수를 결정하고, 일치된 위치의 수를 비교 창 내의 총 위치 수로 나누고, 그 결과에 100을 곱하여 서열 동일성의 백분율을 산출함으로써 계산할 수 있다. 관련 기술 분야의 통상의 기술자는 2개의 서열을 정렬하는데 이용 가능한 많은 확립된 알고리즘이 있음을 인식한다. 비교를 위한 서열의 최적 정렬은 예를 들어 국소 상동성 알고리즘(Smith and Waterman, Adv. Appl. Math., 2:482 [1981]), 상동성 정렬 알고리즘(Needleman and Wunsch, J. Mol. Biol., 48:443 [1970]), 유사성 검색 방법(Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444 [1988]), 이러한 알고리즘의 컴퓨터 구현(예를 들어, GCG 위스콘신 소프트웨어 패키지(Wisconsin Software Package)의 GAP, BESTFIT, FASTA, 및 TFASTA)에 의해, 또는 관련 기술 분야에 공지된 육안 검사에 의해 수행될 수 있다. 서열 동일성 백분율 및 서열 유사성을 결정하는 데 적합한 알고리즘의 예에는 BLAST 및 BLAST 2.0 알고리즘이 포함되지만 이에 국한되지는 않는다(예를 들어, 문헌 [Altschul et al., J. Mol. Biol., 215: 403-410 [1990]]; 및 [Altschul et al., Nucleic Acids Res., 3389-3402 [1977]] 참조). BLAST 분석을 수행하기 위한 소프트웨어는 미국 국립 생물 정보 센터 웹 사이트를 통해 공개적으로 사용할 수 있다. 이 알고리즘은 데이터베이스 서열에서 동일한 길이의 단어와 정렬될 때 양의 값의 역치 점수 "T"와 일치하거나 이를 충족하는, 쿼리 서열에서 길이가 "W"인 짧은 단어를 확인함으로써 고득점 서열 쌍(HSP)을 먼저 확인하는 것을 수반한다. T는 이웃 단어 점수 역치로 지칭된다(상기 문헌 [Altschul et al.] 참조). 이러한 초기 이웃 단어 히트는 이를 포함하는 더 긴 HSP를 찾기 위한 검색을 시작하는 씨드로서 기능한다. 그런 다음, 누적 정렬 점수가 증가할 수 있는 한, 단어 히트는 각각의 서열을 따라 양방향으로 확장된다. 누적 점수는 뉴클레오타이드 서열에 대해 파라미터 "M"(일치하는 잔기 쌍에 대한 보상 점수, 항상 >0) 및 "N"(일치하지 않는 잔기에 대한 페널티 점수, 항상 <0)을 사용하여 계산된다. 아미노산 서열의 경우, 채점 매트릭스를 사용하여 누적 점수를 계산한다. 다음과 같은 경우, 각각의 방향의 단어 히트의 확장이 중단된다: 누적 정렬 점수가 최대 달성 값에서 "X"의 양만큼 떨어질 때; 하나 이상의 음수 채점 잔기 정렬의 누적으로 인해 누적 점수가 0 이하가 될 때; 또는 서열의 끝에 도달할 때. BLAST 알고리즘 파라미터 W, T 및 X는 정렬의 감도 및 속도를 결정한다. BLASTN 프로그램(뉴클레오타이드 서열용)은 디폴트로서 단어 길이(W) 11, 기대값(E) 10, M=5, N=-4 및 두 가닥의 비교를 사용한다. 아미노산 서열의 경우, BLASTP 프로그램은 디폴트로서 단어 길이(W) 3, 기대값(E) 10, BLOSUM62 채점 매트릭스를 사용한다(예를 들어, 문헌 [Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA 89:10915 [1989]] 참조). 서열 정렬 및 서열 동일성 %의 예시적인 결정은 제공된 디폴트 파라미터를 사용하여 GCG 위스콘신 소프트웨어 패키지(Accelrys, 미국 위스콘신주 매디슨 소재)의 BESTFIT 또는 GAP 프로그램을 사용할 수 있다.
"참조 서열"은 서열 비교를 위한 기초로서 사용되는 정의된 서열을 지칭한다. 참조 서열은 더 큰 서열의 서브세트, 예를 들어 전장 유전자 또는 폴리펩타이드 서열의 세그먼트일 수 있다. 일반적으로, 참조 서열은 적어도 20개의 뉴클레오타이드 또는 아미노산 잔기 길이, 적어도 25개의 잔기 길이, 적어도 50개의 잔기 길이, 적어도 100개의 잔기 길이 또는 전장 핵산 또는 폴리펩타이드이다. 2개의 폴리뉴클레오타이드 또는 폴리펩타이드는 각각 (1) 2개의 서열 간에 유사한 서열(즉, 완전한 서열의 일부)을 포함할 수 있고, (2) 2개의 서열 사이에 상이한 서열을 추가로 이해할 수 있기 때문에, 2개(또는 그 초과)의 폴리뉴클레오타이드 또는 폴리펩타이드 간의 서열 비교는 일반적으로 "비교 창"을 통해 2개의 폴리뉴클레오타이드 또는 폴리펩타이드의 서열을 비교하여 서열 유사성의 국소 영역을 확인하고 비교함으로써 수행된다. 일부 실시양태에서, "참조 서열"은 1차 아미노산 서열에 기초할 수 있고, 여기서 참조 서열은 1차 서열에서 하나 이상의 변화를 가질 수 있는 서열이다. 예를 들어, "X123에 상응하는 잔기에 발린을 갖는 서열 번호 686에 기초한 참조 서열"이라는 문구는 서열 번호 686의 위치 X123에 상응하는 잔기(예를 들어, 티로신)가 발린으로 변경된 참조 서열을 지칭하는 것이다.
"비교 창"은 서열이 적어도 20개의 연속적인 뉴클레오타이드 또는 아미노산의 참조 서열과 비교될 수 있는 적어도 약 20개의 연속적인 뉴클레오타이드 위치 또는 아미노산 잔기의 개념적 세그먼트를 지칭하고, 여기서 비교 창 내의 서열 부분은 두 서열의 최적 정렬을 위해 참조 서열(부가 또는 결실을 포함하지 않음)과 비교하여 20% 이하의 부가 또는 결실(즉, 갭)를 포함할 수 있다. 비교 창은 20개보다 긴 연속적인 잔기일 수 있으며, 선택적으로 30, 40, 50, 100개의 창 또는 이보다 긴 창을 포함한다.
주어진 아미노산 또는 폴리뉴클레오타이드 서열의 넘버링의 맥락에서 사용될 때 "~에 상응하는", "~에 대한 참조" 및 "~에 관련된"은 제시된 아미노산 또는 폴리뉴클레오타이드 서열이 참조 서열과 비교될 때 특정 참조 서열의 잔기 넘버링을 지칭한다. 다시 말해서, 제시된 중합체의 잔기 번호 또는 잔기 위치는 제시된 아미노산 또는 폴리뉴클레오타이드 서열 내의 잔기의 실제 수치적 위치가 아니라 참조 서열과 관련하여 지정된다. 예를 들어, 조작된 LDC의 아미노산 서열과 같은 제시된 아미노산 서열은 두 서열 사이의 잔기 일치를 최적화하기 위해 갭을 도입함으로써 참조 서열에 정렬될 수 있다. 이러한 경우, 갭이 존재하지만, 제시된 아미노산 또는 폴리뉴클레오타이드 서열 내의 잔기의 넘버링은 이들 서열이 정렬되는 참조 서열을 기준으로 하여 이루어진다.
"아미노산 차이" 및 "잔기 차이"는 참조 서열 내의 상응하는 위치에서의 아미노산 잔기에 대한, 폴리펩타이드 서열의 위치에서의 아미노산 잔기의 차이를 지칭한다. 아미노산이 상이한 위치는 일반적으로 본원에서 "Xn"으로 지칭되며, 여기서 n은 잔기 차이가 기준으로 하는 참조 서열 내의 상응하는 위치를 지칭한다. 예를 들어, "서열 번호 686과 비교하여 위치 X123에서의 잔기 차이"는 서열 번호 686의 위치 123에 상응하는 폴리펩타이드 위치에서의 아미노산 잔기의 차이를 의미한다. 따라서, 서열 번호 686의 참조 펩타이드가 위치 123에 티로신을 갖는 경우, "서열 번호 686과 비교하여 위치 X123에서의 잔기 차이"는 서열 번호 686의 위치 123에 상응하는 폴리펩타이드의 위치에서 티로신 이외의 다른 임의의 잔기의 아미노산 치환을 지칭한다. 본 명세서에서 대부분의 경우, 한 위치에서의 특정 아미노산 잔기 차이는 "XnY"로 표시되며, 여기서 "Xn"은 참조 폴리펩타이드의 상응하는 잔기 및 위치를 지정하고(상기 기재된 바와 같음), "Y"는 조작된 폴리펩타이드에서 발견되는 아미노산(즉, 참조 폴리펩타이드에서와 상이한 잔기)의 단일 문자 식별자이다. 일부 경우에, 본래의 아미노산은 표시되지 않는다(예를 들어, 109G). 일부 경우에(예를 들어, 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1 및/또는 11-2), 본 개시내용은 또한 통상적인 표기법 "AnB"로 표시되는 특정 아미노산 차이를 제공하며, 여기서 A는 참조 서열 내의 잔기의 단일 문자 식별자이고, n은 참조 서열 내의 잔기 위치의 번호이고, B는 조작된 폴리펩타이드의 서열에서의 잔기 치환의 단일 문자 식별자이다. 일부 예에서, 본 개시내용의 폴리펩타이드는 참조 서열에 대해 잔기 차이가 존재하는 특정 위치의 목록에 의해 표시되는, 참조 서열에 대한 하나 이상의 아미노산 잔기 차이를 포함할 수 있다. 하나 초과의 아미노산이 폴리펩타이드의 특정 잔기 위치에 사용될 수 있는 경우 일부 실시양태에서, 사용될 수 있는 다양한 아미노산 잔기는 "/"에 의해 분리된다(예를 들어, X123F/X123M/X123V 또는 X123F/M/V 또는 123F/M/V). 본 개시내용은 보존적 및 비보존적 아미노산 치환 중 하나 또는 둘 모두를 포함하는 하나 이상의 아미노산 차이를 포함하는 조작된 폴리펩타이드 서열을 포함한다.
용어 "아미노산 치환 세트" 및 "치환 세트"는 폴리펩타이드 서열 내의 아미노산 치환 그룹을 지칭한다. 일부 실시양태에서, 치환 세트는 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15개 또는 그 초과의 아미노산 치환을 포함한다. 일부 실시양태에서, 치환 세트는 실시예의 임의의 표(즉, 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1 및/또는 11-2)에 제시된 임의의 변이체 LDC 폴리펩타이드에 존재하는 아미노산 치환 세트를 지칭한다.
"보존적 아미노산 치환"은 유사한 측쇄를 갖는 상이한 잔기로 잔기를 치환하는 것을 지칭하고, 따라서 전형적으로 동일하거나 유사한 정의된 아미노산 클래스 내의 아미노산으로 폴리펩타이드의 아미노산을 치환하는 것을 포함한다. 비제한적인 예로서, 지방족 측쇄를 갖는 아미노산은 또 다른 지방족 아미노산(예를 들어, 알라닌, 발린, 류신 및 이소류신)으로 치환될 수 있고; 하이드록실 측쇄를 갖는 아미노산은 하이드록실 측쇄를 갖는 또 다른 아미노산(예를 들어, 세린 및 트레오닌)으로 치환되고; 방향족 측쇄를 갖는 아미노산은 방향족 측쇄를 갖는 또 다른 아미노산(예를 들어, 페닐알라닌, 티로신, 트립토판 및 히스티딘)으로 치환되고; 염기성 측쇄를 갖는 아미노산은 염기성 측쇄를 갖는 또 다른 아미노산(예를 들어, 라이신 및 아르기닌)으로 치환되고; 산성 측쇄를 갖는 아미노산은 산성 측쇄를 갖는 또 다른 아미노산(예를 들어, 아스파르트산 또는 글루탐산)으로 치환되고; 소수성 또는 친수성 아미노산은 각각 또 다른 소수성 또는 친수성 아미노산으로 대체된다. 예시적인 보존적 치환은 A, L, V, 또는 I의 다른 지방족 잔기(예를 들어, A, L, V, I) 또는 기타 비극성 잔기(예를 들어, A, L, V, I, G, M)로의 치환; G 또는 M의 다른 비극성 잔기(예를 들어, A, L, V, I, G, M)로의 치환; D 또는 E의 다른 산성 잔기(예를 들어, D, E)로의 치환; K 또는 R의 다른 염기성 잔기(예를 들어, K, R)로의 치환; N, Q, S, T의 다른 극성 잔기(예를 들어, N, Q, S, T)로의 치환; H, Y, W 또는 F의 다른 방향족 잔기(예를 들어, H, Y, W, F)로의 치환; 또는 C 또는 P의 다른 비극성 잔기(예를 들어, C, P)로의 치환을 포함한다.
"비보존적 치환"은 상당히 상이한 측쇄 특성을 갖는 아미노산으로 폴리펩타이드 내의 아미노산을 치환하는 것을 지칭한다. 비보존적 치환은 정의된 그룹 내가 아니라 정의된 그룹 사이의 아미노산을 사용할 수 있으며, 다음과 같은 영향을 미칠 수 있다: (a) 치환 영역의 펩타이드 골격의 구조(예를 들어, 글라이신의 프롤린으로의 치환); (b) 전하 또는 소수성; 및/또는 (c) 측쇄의 벌크. 비제한적인 예로서, 예시적인 비보존적 치환은 염기성 또는 지방족 아미노산으로 치환된 산성 아미노산; 작은 아미노산으로 치환된 방향족 아미노산; 및 소수성 아미노산으로 치환된 친수성 아미노산을 포함한다.
"결실"은 참조 폴리펩타이드로부터 하나 이상의 아미노산을 제거함으로써 이루어지는 폴리펩타이드에 대한 변형을 지칭한다. 결실은 1개 이상의 아미노산, 2개 이상의 아미노산, 5개 이상의 아미노산, 10개 이상의 아미노산, 15개 이상의 아미노산, 또는 20개 이상의 아미노산, 효소 활성을 유지하고/하거나 조작된 류신 데카르복실라제 효소의 개선된 특성을 유지하면서 참조 효소를 구성하는 아미노산의 총 수의 최대 10% 또는 아미노산의 총 수의 최대 20%의 제거를 포함할 수 있다. 결실은 폴리펩타이드의 내부 부분 및/또는 말단 부분으로 유도될 수 있다. 다양한 실시양태에서, 결실은 연속적인 세그먼트를 포함할 수 있거나 불연속적일 수 있다.
"삽입"은 참조 폴리펩타이드로부터 하나 이상의 아미노산의 부가에 의한 폴리펩타이드의 변형을 지칭한다. 삽입은 폴리펩타이드의 내부 부분, 또는 카르복시 또는 아미노 말단에 있을 수 있다. 본원에서 사용되는 삽입은 관련 기술 분야에 공지된 융합 단백질을 포함한다. 삽입은 연속적인 아미노산 세그먼트일 수 있거나, 자연 발생 폴리펩타이드에서 하나 이상의 아미노산에 의해 분리될 수 있다.
"기능적 단편" 및 "생물학적 활성 단편"이라는 용어는 아미노 말단 및/또는 카르복시 말단 결실(들) 및/또는 내부 결실을 갖지만 나머지 아미노산 서열은 그것이 비교되고 있는 서열(예를 들어, 본 발명의 조작된 전장 LDC)의 상응하는 위치와 동일하고 전장 폴리펩타이드의 모든 활성을 실질적으로 보유하는 폴리펩타이드를 지칭한다.
"단리된 폴리펩타이드"는 자연적으로 이에 수반되는 다른 오염물질(예를 들어, 단백질, 지질, 및 폴리뉴클레오타이드)로부터 실질적으로 분리된 폴리펩타이드를 지칭한다. 이 용어는 그의 자연 발생 환경 또는 발현 시스템(예를 들어, 숙주 세포 또는 시험관 내 합성)으로부터 제거되거나 정제된 폴리펩타이드를 포함한다. 재조합 LDC 폴리펩타이드는 세포 내에 존재하거나, 세포 배지에 존재하거나, 용해물 또는 단리된 제제와 같은 다양한 형태로 제조될 수 있다. 이와 같이, 일부 실시양태에서, 본원에서 제공되는 재조합 LDC 폴리펩타이드는 단리된 폴리펩타이드이다.
"실질적으로 순수한 폴리펩타이드"는 폴리펩타이드 종이 존재하는 우세한 종(즉, 몰 또는 중량 기준으로 이는 조성물 내의 임의의 다른 개별 거대분자 종보다 더 풍부함)인 조성물을 지칭하고, 일반적으로 대상 종이 몰 또는 중량% 기준으로 존재하는 거대분자 종의 적어도 약 50%를 차지할 때 실질적으로 정제된 조성물이다. 일반적으로, 실질적으로 순수한 LDC 조성물은 몰 또는 중량% 기준으로 조성물에 존재하는 모든 거대분자 종의 약 60% 이상, 약 70% 이상, 약 80% 이상, 약 90% 이상, 약 95% 이상, 및 약 98% 이상을 차지할 것이다. 일부 실시양태에서, 대상 종은 본질적으로 균일하게 정제되고(즉, 오염물질 종은 통상적인 검출 방법에 의해 조성물에서 검출될 수 없음), 여기서 조성물은 본질적으로 단일 거대분자 종으로 구성된다. 용매 종, 소분자(<500 달톤) 및 원소 이온 종은 거대분자 종으로 간주되지 않는다. 일부 실시양태에서, 단리된 재조합 LDC 폴리펩타이드는 실질적으로 순수한 폴리펩타이드 조성물이다.
"개선된 효소 특성"은 참조 LDC 폴리펩타이드, 예를 들어, 야생형 LDC 폴리펩타이드(예를 들어, 서열 번호 2의 서열을 갖는 야생형 LDC) 또는 또 다른 조작된 LDC 폴리펩타이드와 비교하여 임의의 효소 특성의 개선을 보이는 조작된 LDC 폴리펩타이드를 지칭한다. 개선된 특성에는 단백질 생성 증가, 혈청 안정성 증가, 생체내 혈청 반감기 증가, 열활성 증가, 열안정성 증가, pH 활성 증가, 안정성 증가, 효소 활성 증가, 기질 특이성 및/또는 친화도 증가, 비활성 증가, 기질 및/또는 최종 생성물 억제에 대한 저항성 증가, 화학적 안정성 증가, 화학선택성 개선, 용매 안정성 개선, 산성 pH에 대한 내성 증가, 단백질 분해 활성에 대한 내성 증가(즉, 단백질 분해에 대한 감수성 감소), 응집 감소, 용해도 증가, 면역원성 감소(즉, 면역 반응의 유도 및/또는 유발 능력의 감소), 및 온도 프로팔이의 변경을 포함하지만, 이로 제한되지 않는다.
"증가된 효소 활성" 및 "향상된 촉매 활성"은 참조 LDC 효소(예를 들어, 야생형 LDC 및/또는 또는 또 다른 조작된 LDC)에 비해 비활성(예를 들어, 생산된 생성물/시간/단백질 중량)의 증가 및/또는 기질의 생성물로의 전환 백분율(예를 들어, 특정량의 LDC를 사용하여 특정 시간 동안 출발 기질의 양을 생성물로 전환하는 백분율)의 증가로 표시될 수 있는, 조작된 LDC 폴리펩타이드의 개선된 특성을 지칭한다. 효소 활성을 측정하기 위한 예시적인 방법이 실시예에 제시되어 있다. Km, Vmax 또는 kcat의 고전적인 효소 특성을 포함하는, 효소 활성과 관련된 임의의 특성이 영향을 받을 수 있으며, 이러한 특성의 변화는 효소 활성의 증가로 이어질 수 있다. 효소 활성의 개선은 상응하는 야생형 효소의 효소 활성의 약 1.1배로부터, LDC 폴리펩타이드가 유래되는 자연 발생 LDC 또는 다른 조작된 LDC의 2배, 5배, 10배, 20배, 25배, 50배, 75배, 100배, 150배, 200배 또는 그 초과까지의 효소 활성일 수 있다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 적어도 0.01 μmol/min-mg, 적어도 0.02 μmol/min-mg, 적어도 0.03 μmol/min-mg, 적어도 0.05 μmol/min-mg, 적어도 1.0 μmol/min-mg, 일부 바람직한 실시양태에서 2.0 μmol/min-mg 초과의 비활성을 갖는다. 일부 실시양태에서, Km은 약 1 μM 내지 약 5 mM의 범위; 약 5 μM 내지 약 2 mM의 범위; 약 10 μM 내지 약 2 mM의 범위; 또는 약 10 μM내지 약 1 mM의 범위이다. 일부 특정 실시양태에서, 조작된 LDC 효소는 참조 LDC 효소보다 1.5 내지 10배, 1.5 내지 25배, 1.5 내지 50배, 1.5 내지 100배 또는 그 초과로 개선된 효소 활성을 나타낸다. LDC 활성은 관련 기술 분야에 공지된 임의의 표준 검정에 의해(예를 들어, 반응물의 고갈 또는 생성물 형성을 모니터링함으로써) 측정될 수 있다. 일부 실시양태에서, 생성된 생성물의 양 또는 소모된 기질의 양은 UV 흡광도 또는 질량 스펙트럼 검출과 조합된 고성능 액체 크로마토그래피(HPLC) 분리에 의해 측정된다. 일부 실시양태에서, 효소 활성의 비교는 본원에서 추가로 상세히 기재된 바와 같이, 규정된 효소 제제, 설정된 조건 하에서 규정된 검정, 및 하나 이상의 규정된 기질을 사용하여 이루어진다. 일반적으로, 용해물을 비교할 때, 숙주 세포에서 생성되고 용해물에 존재하는 효소의 양의 변동을 최소화하기 위해 동일한 발현 시스템 및 동일한 숙주 세포의 사용뿐만 아니라, 검정되는 세포의 수 및 단백질의 양이 결정된다.
"증가된 저장 안정성"이라는 어구는 본 발명에 따른 조작된 LDC 폴리펩타이드가 건조된 형태로 생산되고(예를 들어, 동결건조 또는 분무 건조에 의해) 실온보다 높은 온도(예를 들어, 30℃, 37℃, 45℃, 55℃ 등)에서 수일에서 수개월 범위의 기간 동안 보관한 후, 표준 검정(예를 들어, 실시예에서 설명되는 바와 같은)에서 참조 LDC와 비교하여 더 많은 활성을 유지할 것임을 의미한다.
"전환"은 기질(들)의 상응하는 생성물(들)로의 효소에 의한 전환(또는 생체변환)을 지칭한다. "전환율"은 특정 조건에서 일정 기간 내에 제품으로 전환되는 기질의 백분율을 나타낸다. 따라서, LDC 폴리펩타이드의 "효소 활성" 또는 "활성"은 특정 기간에 기질의 생성물로의 "전환율"로 표현될 수 있다.
"혼성화 엄격성"은 핵산의 혼성화에서 세척 조건과 같은 혼성화 조건에 관한 것이다. 일반적으로, 혼성화 반응은 더 낮은 엄격도의 조건에서 수행된 후, 다양하지만 더 높은 엄격도의 세척이 수행된다. 용어 "중등 엄격성 혼성화"는 표적 DNA에 대해 약 60% 동일성, 바람직하게는 약 75% 동일성, 약 85% 동일성을 갖고 표적 폴리펩타이드에 대해 약 90% 초과의 동일성을 갖는 상보적 핵산에 표적 DNA가 결합하도록 허용하는 조건을 지칭한다. 중등 엄격성 조건의 예는 42℃에서 50% 포름아미드, 5x 덴하트(Denhart) 용액, 5x SSPE, 0.2% SDS에서 혼성화한 후, 42℃에서 0.2x SSPE, 0.2% SDS로 세척하는 것과 동등한 조건이다. "높은 엄격성 혼성화"는 일반적으로 정의된 폴리뉴클레오타이드 서열에 대한 용액 조건 하에 결정된 열 용융 온도 Tm으로부터 약 10℃ 이하인 조건을 지칭한다. 일부 실시양태에서, 높은 엄격성 조건은 65℃에서 0.018 M NaCl에서 안정한 하이브리드를 형성하는 핵산 서열만의 혼성화를 허용하는 조건을 지칭한다(즉, 하이브리드가 65℃에서 0.018 M NaCl에서 안정하지 않은 경우, 이는 본 명세서에서 고려되는 바와 같이 높은 엄격성 조건 하에서 안정하지 않을 것이다). 예를 들어, 42℃에서 50% 포름아미드, 5x 덴하트 용액, 5x SSPE, 0.2% SDS에 해당하는 조건에서 혼성화한 후, 65℃에서 0.1x SSPE 및 0.1% SDS로 세척함으로써 높은 엄격성 조건을 제공할 수 있다. 또 다른 높은 엄격성 조건은 65℃에서 0.1% (w:v) SDS를 함유하는 5x SSC에서 혼성하는 것과 동등한 조건에서 혼성화하고 65℃에서 0.1% SDS를 함유하는 0.1x SSC에서 세척하는 것이다. 다른 높은 엄격성 혼성화 조건 및 중등 엄격성 조건은 위에서 인용된 참고 문헌에 설명되어 있다.
"코돈 최적화된"은 코딩된 단백질이 특정 유기체에서 보다 효율적으로 발현되도록 해당 유기체에서 우선적으로 사용되는 것으로, 단백질을 코딩하는 폴리뉴클레오타이드의 코돈이 변하는 것을 지칭한다. 대부분의 아미노산이 "동의어" 또는 "동의성" 코돈이라고 하는 여러 코돈으로 표시되기 때문에 유전자 코드는 축퇴성이지만, 특정 유기체의 코돈 사용 빈도는 무작위가 아니며 특정 코돈 삼중체에 편향되어 있다는 것이 잘 알려져 있다. 이 코돈 사용 빈도 편향은 주어진 유전자, 공통 기능 또는 조상 기원의 유전자, 고도로 발현된 단백질 대 낮은 카피 수 단백질, 및 유기체 게놈의 응집 단백질 코딩 영역과 관련하여 더 높을 수 있다. 일부 실시양태에서, LDC 효소를 코딩하는 폴리뉴클레오타이드는 발현을 위해 선택된 숙주 유기체로부터의 최적의 생산을 위해 최적화된 코돈이다. "제어 서열"은 본원에서 본 개시내용의 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현에 필요하거나 유리한 모든 성분을 포함하는 것을 지칭한다. 각각의 제어 서열은 폴리펩타이드를 코딩하는 핵산 서열에 대해 천연 서열이거나 외래 서열일 수 있다. 이러한 제어 서열은 리더, 폴리아데닐화 서열, 프로펩타이드 서열, 프로모터 서열, 신호 펩타이드 서열, 개시 서열 및 전사 종결자를 포함하지만 이에 제한되지 않는다. 최소한 제어 서열은 프로모터 및 전사 및 번역 정지 신호를 포함한다. 일부 실시양태에서, 제어 서열과 폴리펩타이드를 코딩하는 핵산 서열의 코딩 영역의 라이게이션을 용이하게 하는 특정 제한 부위를 도압하기 위한 링커가 제어 서열에 제공된다.
"작동 가능하게 연결된"은 제어 서열이 관심 폴리펩타이드를 코딩하는 폴리뉴클레오타이드의 발현을 지시하거나 조절하도록 관심 폴리뉴클레오타이드의 상대적인 위치에 제어 서열이 적절하게 배치되는(즉, 기능적인 관계로) 구성으로서 본원에서 정의된다.
"프로모터 서열"은 코딩 서열과 같은 관심 폴리뉴클레오타이드의 발현을 위해 숙주 세포에 의해 인식되는 핵산 서열을 지칭한다. 프로모터 서열은 관심 폴리뉴클레오타이드의 발현을 매개하는 전사 제어 서열을 포함한다. 프로모터는 돌연변이체, 말단 절단 및 하이브리드 프로모터를 포함하는, 선택된 숙주 세포에서 전사 활성을 나타내는 임의의 핵산 서열일 수 있고, 숙주 세포에 대해 상동성 또는 이종성인 세포외 또는 세포내 폴리펩타이드를 코딩하는 유전자로부터 수득될 수 있다.
효소에 의한 전환 반응 과정의 맥락에서 "기질"은 LDC 폴리펩타이드에 의해 작용하는 화합물 또는 분자를 지칭한다. 효소에 의한 전환 과정의 맥락에서 "생성물"은 기질에 대한 LDC 폴리펩타이드의 작용에 의해 생성되는 화합물 또는 분자를 지칭한다.
본원에서 사용되는 바와 같이, 용어 "배양"은 임의의 적합한 배지(예를 들어, 액체, 겔 또는 고체)를 사용하여 적합한 조건 하에 미생물 세포 집단의 성장을 지칭한다.
재조합 폴리펩타이드(예를 들어, LDC 효소 변이체)는 관련 기술 분야에 공지된 임의의 적합한 방법을 사용하여 생성될 수 있다. 예를 들어, 관련 기술 분야의 통상의 기술자에게 잘 알려진 매우 다양한 상이한 돌연변이 유발 기술이 있다. 또한, 돌연변이 유발 키트는 많은 상업적 분자 생물학 공급업체로부터 입수할 수 있다. 정의된 아미노산에서 특이적 치환(부위 지정), 유전자의 국소 영역에서 특이적 또는 무작위 돌연변이(영역 특이적), 또는 전체 유전자에 대한 무작위 돌연변이(예를 들어, 포화 돌연변이 유발)를 만드는 방법을 사용할 수 있다. PCR을 사용한 단일 가닥 DNA 또는 이중 가닥 DNA의 부위 지정 돌연변이 유발, 카세트 돌연변이 유발, 유전자 합성, 오류 빈발 PCR, 셔플링, 및 화학적 포화 돌연변이 유발, 또는 관련 기술 분야에 공지된 임의의 다른 적합한 방법을 포함하지만 이로 제한되지 않는, 효소 변이체를 생성하기 위해 관련 기술 분야의 통상의 기술자에게 많은 적합한 방법이 알려져 있다. DNA 및 단백질 공학에 사용되는 방법의 비제한적인 예는 다음 특허에서 제공된다: 미국 특허 제6,117,679호; 미국 특허 제6,420,175호; 미국 특허 제6,376,246호; 미국 특허 제6,586,182; 미국 특허 제7,747,391호; 미국 특허 제7,747,393호; 미국 특허 제7,783,428호; 및 미국 특허 제8,383,346호. 생성된 후, 변이체는 임의의 원하는 특성(예를 들어, 높거나 증가된 활성, 또는 낮거나 감소된 활성, 증가된 열 활성, 증가된 열 안정성 및/또는 산성 pH 안정성 등)에 대해 스크리닝될 수 있다. 일부 실시양태에서, "재조합 LDC 폴리펩타이드"(본 명세서에서 "조작된 LDC 폴리펩타이드", "변이체 LDC 효소" 및 "LDC 변이체"로도 지칭됨)가 사용된다.
본원에서 사용되는 바와 같이, "벡터"는 DNA 서열을 세포 내로 도입하기 위한 DNA 구축물이다. 일부 실시양태에서, 벡터는 DNA 서열에 의해 코딩되는 폴리펩타이드가 적합한 숙주에서 발현되도록 할 수 있는 적합한 제어 서열에 작동 가능하게 연결된 발현 벡터이다. 일부 실시양태에서, "발현 벡터"는 숙주 세포에서 발현을 유도하기 위해 DNA 서열(예를 들어, 도입유전자)에 작동 가능하게 연결된 프로모터 서열을 갖고, 일부 실시양태에서는 전사 종결자 서열도 포함한다.
본원에서 사용되는 바와 같이, 용어 "발현"은 전사, 전사후 변형, 번역 및 번역후 변형을 포함하지만 이에 제한되지 않는, 폴리펩타이드의 생산에 관련된 임의의 단계를 포함한다. 일부 실시양태에서, 이 용어는 또한 세포로부터 폴리펩타이드의 분비를 포함한다.
본원에서 사용되는 바와 같이, 아미노산 또는 뉴클레오타이드 서열(예를 들어, 프로모터 서열, 신호 펩타이드, 종결자 서열 등)은 이들 서열이 자연에서 회합되지 않는 또 다른 서열에 대해 작동 가능하게 연결될 때, 상기 또 다른 서열에 대해 "이종성"이다.
본원에서 사용되는 바와 같이, 용어 "숙주 세포" 및 "숙주 균주"는 본원에서 제공되는 DNA(예를 들어, 적어도 하나의 LDC 변이체를 코딩하는 폴리뉴클레오타이드 서열)를 포함하는 발현 벡터에 적합한 숙주를 지칭한다. 일부 실시양태에서, 숙주 세포는 관련 기술 분야에 공지된 바와 같은 재조합 DNA 기술을 사용하여 구축된 벡터로 형질전환되거나 형질감염된 원핵 또는 진핵 세포이다.
용어 "유사체"는 참조 폴리펩타이드에 대해 70% 초과 내지 100% 미만의 서열 동일성(예를 들어, 75%, 78%, 80%, 83%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%의 서열 동일성)을 갖는 폴리펩타이드를 의미한다. 일부 실시양태에서, 유사체는 자연 발생 아미노산뿐만 아니라, 호모아르기닌, 오르니틴 및 노르발린을 포함하지만 이에 제한되지 않는 비-자연 발생 아미노산 잔기를 지칭한다. 일부 실시양태에서, 유사체는 또한 1개 이상의 D-아미노산 잔기 및 2개 이상의 아미노산 잔기 사이의 비-펩타이드 결합을 포함한다.
용어 "치료제"는 유익하거나 바람직한 의학적 효과를 갖는, 병리의 징후 또는 증상을 나타내는 대상체에게 투여되는 화합물을 지칭한다.
용어 "약학 조성물"은 약제학적 유효량의 본 발명에 포함되는 조작된 LDC 폴리펩타이드 및 허용되는 담체를 포함하는, 포유동물 대상체(예를 들어, 인간)에서의 약제학적 사용에 적합한 조성물을 지칭한다.
용어 "유전자 요법"은 포유동물 대상체(예를 들어, 인간)에서 질병을 치료 및/또는 예방하기 위한 유전자(즉, 유전 물질)의 사용과 관련하여 사용된다. 일부 실시양태에서, 유전 물질은 포유동물 대상체의 적어도 일부 세포 내로 직접 도입된다. 본 발명이 유전자 요법에 유용한 임의의 특정 방법(들) 또는 조성물(들)로 제한되는 것으로 의도되지 않는다.
용어 "mRNA 요법"은 포유동물 대상체(예를 들어, 인간)에서 질병을 치료 및/또는 예방하기 위한 메신저 RNA(mRNA)의 사용과 관련하여 사용된다. 일부 실시양태에서, 유전 물질은 포유동물 대상체의 적어도 일부 세포 내로 직접 도입된다. 본 발명이 mRNA 요법에 유용한 임의의 특정 방법(들) 또는 조성물(들)로 제한되는 것으로 의도되지 않는다.
"유효량"이라는 용어는 원하는 결과를 생성하기에 충분한 양을 의미한다. 관련 기술 분야의 통상의 기술자는 일상적인 실험을 통해 유효량이 얼마인지 결정할 수 있다.
"단리된" 및 "정제된"이라는 용어는 분자(예를 들어, 단리된 핵산, 폴리펩타이드 등) 또는 자연적으로 회합되는 적어도 하나의 다른 성분으로부터 제거되는 다른 성분을 지칭하기 위해 사용된다. "정제된"이라는 용어는 절대적인 순도를 요구하는 것이 아니라, 상대적인 정의로서 사용된다.
용어 "대상체"는 인간, 비인간 영장류, 가축, 반려 동물, 및 실험 동물(예를 들어, 설치류 및 토끼목)과 같은 포유동물을 포함한다. 이 용어는 수컷뿐만 아니라 암컷도 포함하는 것으로 의도된다.
본원에서 사용되는 바와 같이, 용어 "환자"는 질병에 대해 평가되고 있거나, 치료를 받거나, 겪고 있는 임의의 대상체를 의미한다.
"유아"라는 용어는 생후 1개월에서 대략 1세까지의 아동을 지칭한다. 본 명세서에서 사용되는 바와 같이, "신생아"는 출생부터 생후 28일까지의 아동을 의미한다. "미숙아"라는 용어는 임신 20주 이후에 태어나지만 만삭이 되기 전에 태어난 아기를 말하며, 일반적으로 출생시 체중이 ~500 내지 ~2499 g이다. "초저체중 출생아"는 출생시 체중이 1500 g 미만인 유아이다.
본 명세서에 사용되는 바와 같이, 용어 "아동"은 치료 또는 연구 절차에 동의할 법적 연령에 도달하지 않은 사람을 지칭한다. 일부 실시양태에서, 이 용어는 출생시와 청소년기 사이의 사람을 지칭한다.
본 명세서에 사용된 바와 같이, 용어 "성인"은 관련 관할권에 대한 법적 연령(예를 들어, 미국에서 18세)에 도달한 사람을 지칭한다. 일부 실시양태에서, 이 용어는 임의의 완전히 성장한 성숙한 유기체를 지칭한다. 일부 실시양태에서, 용어 "청소년"은 18세 미만이지만 성적 성숙에 도달한 사람을 지칭한다.
본원에서 사용되는 바와 같이, "조성물" 및 "제제"는 임의의 적합한 용도(예를 들어, 약학 조성물, 식이/영양 보충제, 사료 등)를 위한 본 발명의 적어도 하나의 조작된 LDC를 포함하는 제품을 포함한다.
용어 "투여" 및 "투여하는"은 본 발명의 조성물을 대상체(예를 들어, MSUD의 영향으로 고통받는 사람)에게 제공하는 것을 의미한다.
약학 조성물과 관련하여 사용될 때 용어 "담체"는 임의의 표준 약제학적 담체, 완충제 및 부형제, 예를 들어 안정제, 보존제 및 보조제를 의미한다.
용어 "약학적으로 허용되는"은 임의의 바람직하지 않은 생물학적 효과를 야기하거나 그것이 함유되고 원하는 생물학적 활성을 보유하는 임의의 성분과 유해한 방식으로 상호작용하지 않으면서 대상체에게 투여될 수 있는 물질을 의미한다.
본원에서 사용되는 바와 같이, 용어 "부형제"는 활성 약제학적 성분(API; 예를 들어, 본 발명의 조작된 LDC 폴리펩타이드) 이외의 다른 임의의 약학적으로 허용되는 첨가제, 담체, 희석제, 보조제 또는 기타 성분을 지칭한다. 부형제는 일반적으로 제제 및/또는 투여 목적으로 포함된다.
질병/병태의 증상과 관련하여 사용될 때 용어 "치료 유효량"은 질병/병태의 하나 이상의 증상을 개선, 완화 또는 제거하거나 또는 증상(들)(예를 들어, MSUD)의 발병을 예방 또는 지연하는 화합물(예를 들어, 조작된 LDC 폴리펩타이드)의 양 및/또는 농도를 지칭한다. 일부 실시양태에서, 상기 용어는 연구원, 의사, 수의사 또는 기타 임상의가 찾는 조직, 시스템 또는 동물 대상체에 의한 생물학적(예를 들어, 의학적) 반응을 유도하는 조성물의 양과 관련하여 사용된다.
질병/병태와 관련하여 사용될 때 용어 "치료 유효량"은 질병/병태를 개선, 완화 또는 제거하는 조성물의 양 및/또는 농도를 지칭한다.
용어 "치료하는", "치료하다" 및 "치료"는 완화적 치료뿐만 아니라, 억제적(예를 들어, 예방적) 치료를 포함하는 것으로 의도된다.
본 명세서에서 사용되는 바와 같이, "적어도 하나의"라는 용어는 본 발명을 임의의 특정 수의 항목으로 제한하도록 의도되지 않는다. 이것은 원하는 바에 따라 하나, 둘, 셋, 넷, 다섯, 여섯, 일곱, 여덟, 아홉, 열 또는 그 초과의 항목을 포함하도록 의도된다.
조작된 LDC 폴리펩타이드:
본 발명의 조작된 LDC 폴리펩타이드가 그로부터 유래되는 모 LDC 폴리펩타이드는 플랑크토마이세타세아에 과의 박테리아와 같은 박테리아 균주를 포함한다.
또한, 특정 LDC 변이체(즉, 조작된 LDC 폴리펩타이드)가 야생형 LDC 또는 참조 LDC의 서열에서의 특정 아미노산 잔기의 변형을 참조로 언급될 때, 동등한 위치(들)에서 변형된 또 다른 LDC의 변이체(각각의 아미노산 서열 사이의 선택적인 아미노산 서열 정렬로부터 결정되는)가 본원에 포함되는 것으로 이해된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 조작된 LDC 폴리펩타이드를 생산하는 데 도움이 되는 조건 하에 적어도 하나의 조작된 LDC 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 미생물을 배양함으로써 생산된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 생성된 배양 배지 및/또는 세포로부터 후속적으로 회수된다.
본 발명은 LDC 활성을 갖는 예시적인 조작된 LDC 폴리펩타이드를 제공한다. 실시예는 특정 아미노산 서열 특징을 조작된 LDC 폴리펩타이드의 기능적 활성과 상관시키는 서열 구조 정보를 보여주는 표(즉, 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1, 및/또는 11-2)를 제공한다. 이러한 구조-기능 상관 정보는 서열 번호 2의 참조 조작된 폴리펩타이드에 대한 특정 아미노산 잔기 차이의 형태로 제공될 뿐만 아니라, 예시적인 조작된 LDC 폴리펩타이드에 대한 실험에 의해 결정된 관련 활성 데이터로 제공된다.
일부 실시양태에서, LDC 활성을 갖는 본 발명의 조작된 LDC 폴리펩타이드는 a) 참조 서열 서열 번호 2에 대해 적어도 85%의 서열 동일성을 갖는 아미노산 서열; b) 서열 번호 2와 비교하여 하나 이상의 아미노산 위치에서의 아미노산 잔기 차이; 및 c) 참조 서열과 비교하여 i) 향상된 촉매 활성, ii) 감소된 단백질 분해 민감성, iii) 감소된 응집, iv) 승온에서 동결건조 제제로서의 안정성 증가, v) 감소된 면역원성, 또는 i), ii), iii), iv), 또는 v) 중 임의의 것의 조합으로부터 선택된 개선된 특성을 나타내는 것을 포함한다.
일부 실시양태에서, 본 발명은 조작된 LDC 폴리펩타이드의 기능적 단편을 제공한다. 일부 실시양태에서, 기능적 단편은 그 단편이 유래되는 조작된 LDC 폴리펩타이드(즉, 조작된 모 LDC)의 활성의 적어도 약 90%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%를 차지한다. 일부 실시양태에서, 기능적 단편은 조작된 LDC의 모 서열의 적어도 약 90%, 적어도 약 91%, 적어도 약 92%, 적어도 약 93%, 적어도 약 94%, 적어도 약 95%, 적어도 약 96%, 적어도 약 97%, 적어도 약 98%, 또는 적어도 약 99%를 차지한다. 일부 실시양태에서, 기능적 단편은 5개 미만, 10개 미만, 15개 미만, 20개 미만, 25개 미만, 30개 미만, 35개 미만, 40개 미만, 45개 미만, 및 50개 미만의 아미노산이 말단 절단된다.
일부 실시양태에서, 본 발명은 조작된 LDC 폴리펩타이드의 기능적 단편을 제공한다. 일부 실시양태에서, 기능적 단편은 그 단편이 유래되는 조작된 LDC 폴리펩타이드(즉, 조작된 모 LDC)의 활성의 적어도 약 95%, 96%, 97%, 98%, 또는 99%를 차지한다. 일부 실시양태에서, 기능적 단편은 조작된 LDC의 모 서열의 적어도 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 또는 99%를 차지한다. 일부 실시양태에서, 기능적 단편은 5개 미만, 10개 미만, 15개 미만, 20개 미만, 25개 미만, 30개 미만, 35개 미만, 40개 미만, 45개 미만, 50개 미만, 55개 미만, 60개 미만, 65개 미만, 또는 70개 미만의 아미노산이 말단 절단된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766으로부터 선택되는 참조 서열에 대해 적어도 약 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 동일성을 갖는 아미노산 서열, 또는 임의의 변이체(예를 들어, 실시예에서 제공되는 변이체)의 아미노산 서열을 포함한다. 일부 실시양태에서, 참조 서열은 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766으로부터 선택된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 참조 서열 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766에 대해 적어도 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성, 및 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함한다.
조작된 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 발현 벡터 및 숙주 세포:
본 발명은 본원에서 설명되는 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 제공한다. 일부 실시양태에서, 폴리뉴클레오타이드는 폴리펩타이드를 발현할 수 있는 재조합 폴리뉴클레오타이드를 생성하기 위해 유전자 발현을 제어하는 하나 이상의 이종성 조절 서열에 작동 가능하게 연결된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드(들)를 코딩하는 적어도 하나의 이종성 폴리뉴클레오타이드를 함유하는 발현 구축물은 상응하는 LDC 폴리펩타이드(들)를 발현하기 위해 적절한 숙주 세포 내로 도입된다.
관련 기술 분야의 통상의 기술자에게 명백한 바와 같이, 단백질 서열의 이용 가능성 및 다양한 아미노산에 상응하는 코돈에 대한 지식은 대상 폴리펩타이드를 코딩할 수 있는 모든 폴리뉴클레오타이드에 대한 설명을 제공한다. 동일한 아미노산이 대체 또는 동의성 코돈에 의해 코딩되는 유전자 코드의 축퇴성은 매우 많은 수의 핵산이 만들어지도록 하며, 이들 핵산은 모두 조작된 LDC 폴리펩타이드를 코딩한다. 따라서, 본 발명은 가능한 코돈 선택을 기초로 하여 조합을 선택함으로써 본원에서 설명되는 LDC 폴리펩타이드를 코딩하는, 제조될 수 있는 LDC 폴리뉴클레오타이드의 각각의 모든 가능한 변이의 생성을 위한 방법 및 조성물을 제공하며, 이러한 모든 변이는 실시예에서(예를 들어, 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1 및/또는 11-2에서) 제시되는 아미노산 서열을 포함하는, 본원에서 설명되는 기술된 임의의 폴리펩타이드에 대해 구체적으로 개시된 것으로 고려되어야 한다.
일부 실시양태에서, 코돈은 바람직하게는 단백질 생산을 위해 선택된 숙주 세포에 의한 이용을 위해 최적화된다. 예를 들어, 박테리아에서 사용되는 바람직한 코돈은 일반적으로 박테리아에서의 발현에 사용된다. 따라서, 조작된 LDC 폴리펩타이드를 코딩하는 코돈 최적화된 폴리뉴클레오타이드는 전장 코딩 영역에서 코돈 위치의 약 40%, 50%, 60%, 70%, 80%, 90%, 또는 90% 초과의 위치에 바람직한 코돈을 함유한다.
일부 실시양태에서, LDC 폴리뉴클레오타이드는 본원에서 개시되는 특성을 갖는 LDC 활성을 갖는 조작된 폴리펩타이드를 코딩하고, 여기서 폴리펩타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766으로부터 선택되는 참조 서열에 대해 적어도 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 동일성을 갖는 아미노산 서열, 또는 임의의 변이체(예를 들어, 실시예에서 제공되는 변이체)의 아미노산 서열, 및 서열 번호 1, 3, 5, 7, 9, 11, 13, 37, 233, 283, 483, 593, 685, 687, 및/또는 765의 참조 폴리뉴클레오타이드와 비교하여 하나 이상의 잔기 차이, 또는 실시예에서 개시되는 임의의 변이체(예를 들어, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10개 또는 그 초과의 아미노산 잔기 위치)의 아미노산 서열을 포함한다.
일부 실시양태에서, LDC 폴리뉴클레오타이드는 본원에서 개시되는 특성을 갖는 LDC 활성을 갖는 조작된 폴리펩타이드를 코딩하고, 여기서 폴리펩타이드는 참조 서열 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766에 대해 적어도 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% 또는 그 초과의 서열 동일성, 및 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함한다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 서열 번호 1, 3, 5, 7, 9, 11, 13, 37, 233, 283, 483, 593, 685, 687 및/또는 765로부터 선택되는 폴리뉴클레오타이드 서열로부터 선택되는 폴리뉴클레오타이드 서열을 포함한다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 서열 번호 1, 3, 5, 7, 9, 11, 13, 37, 233, 283, 483, 593, 685, 687 및/또는 765에 대해 적어도 80%, 85%, 90%, 93%, 95%, 96%, 97%, 98%, 99%의 뉴클레오타이드 잔기 동일성을 갖는다.
일부 실시양태에서, 폴리뉴클레오타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766으로부터 선택되는 참조 폴리뉴클레오타이드 서열, 또는 그의 상보체, 또는 본원에서 제공되는 임의의 변이체 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열에 높은 엄격성 조건 하에 혼성화할 수 있다. 일부 실시양태에서, 높은 엄격성 조건 하에 혼성화할 수 있는 폴리뉴클레오타이드는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688 및/또는 766과 비교하여 하나 이상의 잔기 차이를 갖는 아미노산 서열을 포함하는 LDC 폴리펩타이드를 코딩한다.
일부 실시양태에서, 본원의 임의의 조작된 LDC 폴리펩타이드를 코딩하는 단리된 폴리뉴클레오타이드는 LDC 폴리펩타이드의 발현을 용이하게 하기 위해 다양한 방식으로 조작된다. 일부 실시양태에서, LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드는 LDC 폴리뉴클레오타이드 및/또는 폴리펩타이드의 발현을 조절하기 위해 하나 이상의 제어 서열이 존재하는 발현 벡터를 포함한다. 단리된 폴리뉴클레오타이드를 벡터에 삽입하기 전에 조작하는 것은 사용되는 발현 벡터에 따라 바람직하거나 필요할 수 있다. 재조합 DNA 방법을 사용하여 폴리뉴클레오타이드 및 핵산 서열을 변형하는 기술은 관련 기술 분야에 잘 알려져 있다. 일부 실시양태에서, 제어 서열은 특히 프로모터, 리더 서열, 폴리아데닐화 서열, 프로펩타이드 서열, 신호 펩타이드 서열, 및 전사 종결자를 포함한다. 일부 실시양태에서, 적합한 프로모터는 숙주 세포 선택을 기초로 하여 선택된다. 박테리아 숙주 세포의 경우, 본 개시내용의 핵산 구축물의 전사를 지시하기 위한 적합한 프로모터는 이. 콜라이 lac 오페론, 스트렙토마이세스 코엘리콜로라 아가라제 유전자(dagA), 바실러스 서브틸리스 레반수크라제 유전자(sacB), 스트렙토마이세스 코엘리콜로르(Streptomyces coelicolor) 아가라제 유전자(dagA), 바실러스 서브틸리스(Bacillus subtilis) 레반수크라제 유전자(sacB), 바실러스 리케니포르미스(Bacillus licheniformis) 알파-아밀라아제 유전자(amyL), 바실러스 스테아로테르모필루스(Bacillus stearothermophilus) 말토제닉 아밀라아제 유전자(amyM), 바실러스 아밀로리퀘파시엔스(Bacillus amyloliquefaciens) 알파-아밀라아제 유전자(amyQ), 바실러스 리케니포르미스 페니실리나제 유전자(penP), 바실러스 서브틸리스 xylA 및 xylB 유전자, 및 원핵 베타-락타마제 유전자로부터 얻은 프로모터(예를 들어, 문헌 [Villa-Kamaroff et al., Proc. Natl Acad. Sci. USA 75:3727-3731 [1978]] 참조), 및 tac 프로모터(예를 들어, 문헌 [DeBoer et al., Proc. Natl Acad. Sci. USA80: 21-25 [1983]] 참조)를 포함하지만, 이로 제한되지 않는다. 사상 진균 숙주 세포에 대한 예시적인 프로모터는 아스페르길루스 오리자에(Aspergillus oryzae) TAKA 아밀라아제, 리조무코르 미에헤이(Rhizomucor miehei) 아스파르트산 프로테이나제, 아스페르길루스 니게르(Aspergillus niger) 중성 알파-아밀라아제, 아스페르길루스 니게르 산 안정성 알파-아밀라아제, 아스페르길루스 니게르 또는 아스페르길루스 아외모리(Aspergillus awamori) 글루코아밀라아제(glaA), 리조무코르 미에헤이 리파제, 아스페르길루스 오리자에 알칼리성 프로테아제, 아스페르길루스 오리자에 트리오스 포스페이트 이소머라제, 아스페르길루스 니둘란스(Aspergillus nidulans) 아세트아미다제 및 푸사리움 옥시스포룸(Fusarium oxysporum) 트립신 유사 프로테아제로부터 얻은 프로모터(예를 들어, WO 96/00787 참조), 및 NA2-tpi 프로모터(아스페르길루스 니게르 중성 알파-아밀라아제 및 아스페르길루스 오리자에 트리오스 포스페이트 이소머라제에 대한 유전자로부터 얻은 프로모터의 하이브리드), 및 이들의 돌연변이체, 말단 절단체 및 하이브리드 프로모터를 포함하지만, 이로 제한되지 않는다. 예시적인 효모 세포 프로모터는 사카로마이세스 세레비지아에(Saccharomyces cerevisiae) 에놀라제(ENO-1), 사카로마이세스 세레비지아에 갈락토키나제(GAL1), 사카로마이세스 세레비지아에 알콜 데하이드로게나제/글리세르알데히드-3-포스페이트 데하이드로게나제(ADH2/GAP), 및 사카로마이세스 세레비지아에 3-포스포글리세레이트 키나제에 대한 유전자로부터 유래될 수 있다. 효모 숙주 세포에 대한 다른 유용한 프로모터는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., Yeast 8:423-488 [1992]] 참조).
일부 실시양태에서, 제어 서열은 또한 적합한 전사 종결자 서열(즉, 전사를 종결시키기 위해 숙주 세포에 의해 인식되는 서열)이다. 일부 실시양태에서, 종결자 서열은 LDC 폴리펩타이드를 코딩하는 핵산 서열의 3' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능하는 임의의 적합한 종결자가 본 발명에서 사용된다. 사상 진균 숙주 세포에 대한 예시적인 전사 종결자는 아스페르길루스 오리자에 TAKA 아밀라아제, 아스페르길루스 니게르 글루코아밀라아제, 아스페르길루스 니둘란스 안트라닐레이트 신타제, 아스페르길루스 니게르 알파-글루코시다제, 및 푸사리움 옥시스포룸 트립신 유사 프로테아제에 대한 유전자로부터 얻을 수 있다. 효모 숙주 세포에 대한 예시적인 종결자는 사카로마이세스 세레비지아에 에놀라제, 사카로마이세스 세레비지아에 시토크롬 C(CYC1) 및 사카로마이세스 세레비지아에 글리세르알데히드-3-포스페이트 데하이드로게나제에 대한 유전자로부터 얻을 수 있다. 효모 숙주 세포에 대한 다른 유용한 종결자는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Romanos et al., 상기 문헌] 참조).
일부 실시양태에서, 제어 서열은 또한 적합한 리더 서열(즉, 숙주 세포에 의한 번역에 중요한 mRNA의 비번역 영역)이다. 일부 실시양태에서, 리더 서열은 LDC 폴리펩타이드를 코딩하는 핵산 서열의 5' 말단에 작동 가능하게 연결된다. 선택된 숙주 세포에서 기능하는 임의의 적합한 리더 서열이 본 발명에서 사용된다. 사상 진균 숙주 세포에 대한 예시적인 리더는 아스페르길루스 오리자에 TAKA 아밀라아제 및 아스페르길루스 니둘란스 트리오스 포스페이트 이소머라제에 대한 유전자로부터 얻는다. 효모 숙주 세포에 적합한 리더는 사카로마이세스 세레비지아에 에놀라제(ENO-1), 사카로마이세스 세레비지아에 3-포스포글리세레이트 키나제, 사카로마이세스 세레비지아에 알파-인자, 및 사카로마이세스 세레비지아에 알콜 데하이드로게나제/글리세르알데히드-3-포스페이트 데하이드로게나제(ADH2/GAP)로부터 얻는다.
일부 실시양태에서, 제어 서열은 또한 폴리아데닐화 서열(즉, 핵산 서열의 3' 말단에 작동 가능하게 연결되고, 전사될 때 폴리아데노신을 전사되는 mRNA에 첨가하라는 신호로서 숙주 세포에 의해 인식되는 서열)이다. 선택한 숙주 세포에서 기능하는 임의의 적합한 폴리아데닐화 서열이 본 발명에서 사용된다. 사상 진균 숙주 세포에 대한 예시적인 폴리아데닐화 서열은 아스페르길루스 오리자에 TAKA 아밀라아제, 아스페르길루스 니게르 글루코아밀라아제, 아스페르길루스 니둘란스 안트라닐레이트 신타제, 푸사리움 옥시스포룸 트립신 유사 프로테아제 및 아스페르길루스 니게르 알파-글루코시다제에 대한 유전자로부터의 서열을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 대한 유용한 폴리아데닐화 서열이 알려져 있다(예를 들어, 문헌 [Guo and Sherman, Mol. Cell. Bio., 15:5983-5990 [1995]] 참조).
일부 실시양태에서, 제어 서열은 또한 신호 펩타이드(즉, 폴리펩타이드의 아미노 말단에 연결된 아미노산 서열을 코딩하고 코딩된 폴리펩타이드를 세포의 분비 경로로 지시하는 코딩 영역)이다. 일부 실시양태에서, 핵산 서열의 코딩 서열의 5' 말단은 분비되는 폴리펩타이드를 코딩하는 코딩 영역의 세그먼트에 대해 번역 판독 프레임으로 자연적으로 연결된 신호 펩타이드 코딩 영역을 본래 함유한다. 대안적으로, 일부 실시양태에서, 코딩 서열의 5' 말단은 코딩 서열에 대해 외인성인 신호 펩타이드 코딩 영역을 함유한다. 발현된 폴리펩타이드를 선택된 숙주 세포의 분비 경로로 지시하는 임의의 적합한 신호 펩타이드 코딩 영역은 조작된 폴리펩타이드(들)의 발현을 위해 사용된다. 박테리아 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 바실러스 NCIB 11837 말토제닉 아밀라아제, 바실러스 스테아로테르모필루스 알파-아밀라아제, 바실러스 리케니포르미스 서브틸리신, 바실러스 리케니포르미스 베타-락타마제, 바실러스 스테아로테르모필루스 중성 프로테아제(nprT, nprS, nprM) 및 바실러스 서브틸리스 prsA에 대한 유전자로부터 얻은 것을 포함하지만, 이로 제한되지 않는다. 추가의 신호 펩타이드는 관련 기술 분야에 공지되어 있다(예를 들어, 문헌 [Simonen and Palva, Microbiol. Rev., 57:109-137 [1993]] 참조). 일부 실시양태에서, 사상 진균 숙주 세포에 대한 효과적인 신호 펩타이드 코딩 영역은 아스페르길루스 오리자에 TAKA 아밀라아제, 아스페르길루스 니게르 중성 아밀라아제, 아스페르길루스 니게르 글루코아밀라아제, 리조무코르 미에헤이 아스파르트산 프로테이나제, 후미콜라 인솔렌스(Humicola insolens) 셀룰라아제, 및 후미콜라 라누기노사(Humicola lanuginosa) 리파제에 대한 유전자로부터 얻은 신호 펩타이드 코딩 영역을 포함하지만, 이로 제한되지 않는다. 효모 숙주 세포에 유용한 신호 펩타이드는 사카로마이세스 세레비지아에 알파-인자 및 사카로마이세스 세레비지아에 인버타제에 대한 유전자로부터 얻은 신호 펩타이드를 포함하지만, 이에 제한되지는 않는다.
일부 실시양태에서, 제어 서열은 또한 폴리펩타이드의 아미노 말단에 위치한 아미노산 서열을 코딩하는 프로펩타이드 코딩 영역이다. 생성된 폴리펩타이드는 "프로엔자임", "프로폴리펩타이드" 또는 "자이모겐"으로 지칭된다. 프로폴리펩타이드는 프로펩타이드로부터 프로펩타이드의 촉매에 의한 또는 자가촉매에 의한 절단에 의해 성숙한 활성 폴리펩타이드로 전환될 수 있다. 프로펩타이드 코딩 영역은 바실러스 서브틸리스 알칼리성 프로테아제(aprE), 바실러스 서브틸리스 중성 프로테아제(nprT), 사카로마이세스 세레비지아에 알파-인자, 리조무코르 미에헤이 아스파르트산 프로테이나제 및 미셀리오프토라 테르모필라(Myceliophthora thermophila) 락타제에 대한 유전자를 포함하지만 이로 제한되지 않는 임의의 적합한 공급원으로부터 얻을 수 있다(예를 들어, WO 95/33836 참조). 신호 펩타이드와 프로펩타이드 영역이 모두 폴리펩타이드의 아미노 말단에 존재하는 경우, 프로펩타이드 영역은 폴리펩타이드의 아미노 말단 옆에 위치하고, 신호 펩타이드 영역은 프로펩타이드 영역의 아미노 말단 옆에 위치한다.
일부 실시양태에서, 조절 서열이 또한 이용된다. 이들 서열은 숙주 세포의 성장과 관련된 폴리펩타이드의 발현 조절을 용이하게 한다. 조절 시스템의 예는 조절 화합물의 존재를 포함하는, 화학적 또는 물리적 자극에 반응하여 유전자의 발현을 켜거나 끄는 시스템이다. 원핵 숙주 세포에서, 적합한 조절 서열은 lac, tac 및 trp 오퍼레이터 시스템을 포함하지만, 이에 제한되지는 않는다. 효모 숙주 세포에서, 적절한 조절 시스템은 ADH2 시스템 또는 GAL1 시스템을 포함하지만, 이에 제한되지는 않는다. 사상 진균에서, 적합한 조절 서열은 TAKA 알파-아밀라아제 프로모터, 아스페르길루스 니게르 글루코아밀라아제 프로모터, 및 아스페르길루스 오리자에 글루코아밀라아제 프로모터를 포함하지만, 이에 제한되지는 않는다.
또 다른 측면에서, 본 발명은 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드, 및 그들이 도입되는 숙주의 유형에 따라 프로모터 및 종결자, 복제 기점 등과 같은 하나 이상의 발현 조절 영역을 포함하는 재조합 발현 벡터에 관한 것이다. 일부 실시양태에서, 본원에서 설명되는 다양한 핵산 및 제어 서열은 함께 결합되어 그 부위에서 LDC 폴리펩타이드를 코딩하는 핵산 서열의 삽입 또는 치환을 허용하는 하나 이상의 편리한 제한 부위를 포함하는 재조합 발현 벡터를 생성한다. 대안적으로, 일부 실시양태에서, 본 발명의 핵산 서열은 발현을 위한 적절한 벡터 내에 핵산 서열 또는 이 서열을 포함하는 핵산 구축물을 삽입함으로써 발현된다. 발현 벡터의 생성을 포함하는 일부 실시양태에서, 코딩 서열은 코딩 서열이 발현을 위한 적절한 제어 서열과 작동 가능하게 연결되도록 벡터에 위치된다.
재조합 발현 벡터는 재조합 DNA 절차에 편리하게 적용되고 LDC 폴리뉴클레오타이드 서열의 발현을 유도할 수 있는 임의의 적합한 벡터(예를 들어, 플라스미드 또는 바이러스)일 수 있다. 벡터의 선택은 일반적으로 그 내부로 벡터가 도입되는 숙주 세포와 벡터의 상용성에 달려 있다. 벡터는 선형 또는 폐쇄형 원형 플라스미드일 수 있다.
일부 실시양태에서, 발현 벡터는 자가 복제 벡터(즉, 염색체외 엔티티로서 존재하고, 그의 복제는 염색체 복제와 무관한 벡터, 예를 들어 플라스미드, 염색체외 요소, 미니염색체 또는 인공 염색체)이다. 벡터에는 자가 복제를 보장하기 위한 임의의 수단이 포함될 수 있다. 일부 대안적인 실시양태에서, 벡터는 숙주 세포 내로 도입될 때 게놈 내로 통합되고 통합된 염색체(들)와 함께 복제되는 것이다. 또한, 일부 실시양태에서, 단일 벡터 또는 플라스미드, 또는 숙주 세포의 게놈 내로 도입될 전체 DNA를 함께 함유하는 둘 이상의 벡터 또는 플라스미드, 및/또는 트랜스포존이 이용된다.
일부 실시양태에서, 발현 벡터는 형질전환된 세포의 용이한 선택을 허용하는 하나 이상의 선택 가능한 마커를 함유한다. "선택 가능한 마커"는 그의 생성물이 살생물제 또는 바이러스 저항성, 중금속에 대한 저항성, 영양요구성에 대한 독립영양성화 등을 제공하는 유전자이다. 박테리아 선택 가능 마커의 예는 바실러스 서브틸리스 또는 바실러스 리케니포르미스로부터의 dal 유전자, 또는 암피실린, 카나마이신, 클로람페니콜 또는 테트라사이클린 내성과 같은 항생제 내성을 부여하는 마커를 포함하지만 이에 제한되지 않는다. 효모 숙주 세포에 적합한 마커는 ADE2, HIS3, LEU2, LYS2, MET3, TRP1 및 URA3을 포함하지만, 이에 제한되지는 않는다. 사상 진균 숙주 세포에서 사용하기 위한 선택 가능한 마커는 amdS(아세트아미다제, 예를 들어, 에이. 니둘란스 또는 에이. 오리자에 유래의 것), argB(오르니틴 카르바모일트랜스퍼라제), bar(포스피노트리신 아세틸트랜스퍼라제, 예를 들어 에스. 히그로스코피쿠스(S. hygroscopicus) 유래의 것), hph(히그로마이신 포스포트랜스퍼라제), niaD(니트레이트 리덕타제), pyrG(오로티딘-5'-포스페이트 데카르복실라제, 예를 들어 에이. 니둘란스 또는 에이. 오리자에 유래의 것), sC(설페이트 아데닐트랜스퍼라제), 및 trpC(안트라닐레이트 신타제) 및 이들의 동등물을 포함하지만, 이에 제한되지는 않는다. 또 다른 측면에서, 본 발명은 본 발명의 적어도 하나의 조작된 LDC 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함하는 숙주 세포를 제공하며, 여기서 폴리뉴클레오타이드(들)는 숙주 세포에서 조작된 LDC 효소(들)의 발현을 위한 하나 이상의 제어 서열에 작동 가능하게 연결된다. 본 발명의 발현 벡터에 의해 코딩되는 폴리펩타이드를 발현하는데 사용하기에 적합한 숙주 세포는 관련 기술 분야에 잘 알려져 있으며, 박테리아 세포, 예를 들어 이. 콜라이, 비브리오 플루비알리스(Vibrio fluvialis), 스트렙토마이세스 및 살모넬라 티피무리움(Salmonella typhimurium) 세포; 진균 세포, 예를 들어 효모 세포(예를 들어, 사카로마이세스 세레비지아에 또는 피키아 파스토리스(Pichia pastoris)(ATCC 수탁 번호 201178)); 곤충 세포, 예를 들어 드로소필라(Drosophila) S2 및 스포돕테라(Spodoptera) Sf9 세포; 동물 세포, 예를 들어 CHO, COS, BHK, 293 및 보우스(Bowes) 흑색종 세포; 및 식물 세포를 포함하지만, 이에 제한되지는 않는다. 예시적인 숙주 세포는 또한 다양한 에셔리키아 콜라이(Escherichia coli) 균주(예를 들어, W3110(ΔfhuA) 및 BL21)를 포함한다.
따라서, 또 다른 측면에서, 본 발명은 조작된 LDC 폴리펩타이드를 생산하는 방법을 제공하며, 여기서 상기 방법은 폴리펩타이드의 발현에 적합한 조건 하에 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 발현할 수 있는 숙주 세포를 배양하는 것을 포함한다. 일부 실시양태에서, 상기 방법은 본원에서 설명되는 바와 같이 LDC 폴리펩타이드를 단리 및/또는 정제하는 단계를 추가로 포함한다.
숙주 세포에 대한 적절한 배양 배지 및 성장 조건은 관련 기술 분야에 잘 알려져 있다. LDC 폴리펩타이드의 발현을 위한 폴리뉴클레오타이드를 세포 내로 도입하기 위한 임의의 적합한 방법이 본 발명에서 유용할 것으로 고려된다. 적합한 기술은 전기천공, 바이올리스틱(biolistic) 입자 충격, 리포솜 매개 형질감염, 염화칼슘 형질감염 및 원형질체 융합을 포함하지만, 이에 제한되지는 않는다.
본원에서 개시되는 특성을 갖는 조작된 LDC 폴리펩타이드는 자연 발생 또는 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 관련 기술 분야에 공지된 및/또는 본원에서 설명되는 바와 같은 임의의 적합한 돌연변이 유발 및/또는 유도 진화 방법에 적용함으로써 수득될 수 있다. 예시적인 유도 진화 기술은 돌연변이 유발 및/또는 DNA 셔플링이다(예를 들어, 문헌 [Stemmer, Proc. Natl. Acad. Sci. USA 91:10747-10751 [1994]]; WO 95/22625; WO 97/0078; WO 97/35966; WO 98/27230; WO 00/42651; WO 01/75767 및 미국 특허 제6,537,746호 참조). 사용될 수 있는 다른 유도 진화 절차에는 특히 엇갈린 연장 과정(StEP: Staggered extension process), 시험관내 재조합(예를 들어, 문헌 [Zhao et al., Nat. Biotechnol., 16:258-261 [1998]] 참조), 돌연변이 유발성 PCR(예를 들어, 문헌 [Caldwell et al., PCR Methods Appl., 3:S136-S140 [1994]] 참조) 및 카세트 돌연변이 유발(예를 들어, 문헌 [Black et al., Proc. Natl. Acad. Sci. USA 93:3525-3529 [1996]] 참조)이 포함된다.
돌연변이 유발 및 유도 진화 방법은 발현, 스크리닝 및 검정될 수 있는 변이체 라이브러리를 생성하기 위해 LDC 코딩 폴리뉴클레오타이드에 쉽게 적용될 수 있다. 임의의 적절한 돌연변이 유발 및 유도 진화 방법이 본 발명에서 사용될 수 있고, 관련 기술 분야에 잘 알려져 있다 (예를 들어, 미국 특허 5,605,793, 5,811,238, 5,830,721, 5,834,252, 5,837,458, 5,928,905, 6,096,548, 6,117,679, 6,132,970, 6,165,793, 6,180,406, 6,251,674, 6,265,201, 6,277,638, 6,287,861, 6,287,862, 6,291,242, 6,297,053, 6,303,344, 6,309,883, 6,319,713, 6,319,714, 6,323,030, 6,326,204, 6,335,160, 6,335,198, 6,344,356, 6,352,859, 6,355,484, 6,358,740, 6,358,742, 6,365,377, 6,365,408, 6,368,861, 6,372,497, 6,337,186, 6,376,246, 6,379,964, 6,387,702, 6,391,552, 6,391,640, 6,395,547, 6,406,855, 6,406,910, 6,413,745, 6,413,774, 6,420,175, 6,423,542, 6,426,224, 6,436,675, 6,444,468, 6,455,253, 6,479,652, 6,482,647, 6,483,011, 6,484,105, 6,489,146, 6,500,617, 6,500,639, 6,506,602, 6,506,603, 6,518,065, 6,519,065, 6,521,453, 6,528,311, 6,537,746, 6,573,098, 6,576,467, 6,579,678, 6,586,182, 6,602,986, 6,605,430, 6,613,514, 6,653,072, 6,686,515, 6,703,240, 6,716,631, 6,825,001, 6,902,922, 6,917,882, 6,946,296, 6,961,664, 6,995,017, 7,024,312, 7,058,515, 7,105,297, 7,148,054, 7,220,566, 7,288,375, 7,384,387, 7,421,347, 7,430,477, 7,462,469, 7,534,564, 7,620,500, 7,620,502, 7,629,170, 7,702,464, 7,747,391, 7,747,393, 7,751,986, 7,776,598, 7,783,428, 7,795,030, 7,853,410, 7,868,138, 7,783,428, 7,873,477, 7,873,499, 7,904,249, 7,957,912, 7,981,614, 8,014,961, 8,029,988, 8,048,674, 8,058,001, 8,076,138, 8,108,150, 8,170,806, 8,224,580, 8,377,681, 8,383,346, 8,457,903, 8,504,498, 8,589,085, 8,762,066, 8,768,871, 9,593,326, 9,665,694, 9,684,771, 및 모든 관련 미국 및 미국외 국가 대응 특허; 문헌 [Ling et al., Anal. Biochem., 254(2):157-78 [1997]; Dale et al., Meth. Mol. Biol., 57:369-74 [1996]; Smith, Ann. Rev. Genet., 19:423-462 [1985]; Botstein et al., Science, 229:1193-1201 [1985]; Carter, Biochem. J., 237:1-7 [1986]; Kramer et al., Cell, 38:879-887 [1984]; Wells et al., Gene, 34:315-323 [1985]; Minshull et al., Curr. Op. Chem. Biol., 3:284-290 [1999]; Christians et al., Nat. Biotechnol., 17:259-264 [1999]; Crameri et al., Nature, 391:288-291 [1998]; Crameri, et al., Nat. Biotechnol., 15:436-438 [1997]; Zhang et al., Proc. Nat. Acad. Sci. U.S.A., 94:4504-4509 [1997]; Crameri et al., Nat. Biotechnol., 14:315-319 [1996]; Stemmer, Nature, 370:389-391 [1994]; Stemmer, Proc. Nat. Acad. Sci. USA, 91:10747-10751 [1994]; WO 95/22625; WO 97/0078; WO 97/35966;WO 98/27230; WO 00/42651; WO 01/75767; WO 2009/152336; 및 미국 특허 출원 공개 2011/0082055, 2014/0005057, 2014/0214391, 2014/0221216, 2015/0133307, 2015/0134315, 및 2015/0050658 참조; 이들은 모두 본 명세서에 참고로 포함됨).
일부 실시양태에서, 돌연변이 유발 처리 후에 수득된 효소 클론은 효소 제제를 정의된 온도(또는 다른 검정 조건)에 적용하고, 열처리 또는 다른 적합한 검정 조건 후에 남아있는 효소 활성의 양을 측정함으로써 스크리닝된다. 그런 다음, LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 포함하는 클론을 유전자로부터 분리하고, 뉴클레오타이드 서열 변화(있는 경우)를 확인하기 위해 서열을 분석하고, 숙주 세포에서 효소를 발현하는 데 사용한다. 발현 라이브러리로부터 효소 활성을 측정하는 것은 관련 기술 분야에 공지된 임의의 적합한 방법(예를 들어, 표준 생화학 기술, 예를 들어 HPLC 분석)을 사용하여 수행될 수 있다.
공지된 서열의 조작된 폴리펩타이드의 경우, 효소를 코딩하는 폴리뉴클레오타이드는 공지된 합성 방법에 따라 표준 고체상 방법에 의해 제조될 수 있다. 일부 실시양태에서, 약 100개 이하의 염기의 단편이 개별적으로 합성된 후, 연결되어(예를 들어, 효소 또는 화학적 라이게이션 방법, 또는 폴리머라제 매개 방법에 의해) 임의의 원하는 연속 서열을 형성할 수 있다. 예를 들어, 본 명세서에서 개시되는 폴리뉴클레오타이드 및 올리고뉴클레오타이드는 고전적 포스포르아미다이트 방법을 사용하여 화학적 합성에 의해 제조될 수 있고(예를 들어, 문헌 [Beaucage et al., Tet. Lett., 22:1859-69 [1981]]; 및 [Matthes et al., EMBO J., 3:801-05 [1984]] 참조), 이것은 상기 방법이 자동 합성 방법에서 일반적으로 실행되기 때문이다. 포스포르아미다이트 방법에 따라, 올리고뉴클레오타이드가 합성된다(예를 들어, 자동 DNA 합성기에서 합성되고, 정제, 어닐링, 라이게이션되고, 적절한 벡터에 클로닝된다).
따라서, 일부 실시양태에서, 조작된 LDC 폴리펩타이드를 제조하는 방법은 (a) 본원에서 설명되는 바와 같은 임의의 변이체의 아미노산 서열로부터 선택되는 아미노산 서열을 포함하는 폴리펩타이드를 코딩하는 폴리뉴클레오타이드를 합성하는 단계, 및 (b) 폴리뉴클레오타이드에 의해 코딩되는 LDC 폴리펩타이드를 발현시키는 단계를 포함한다. 상기 방법의 일부 실시양태에서, 폴리뉴클레오타이드에 의해 코딩되는 아미노산 서열은 선택적으로 하나 또는 여러 개(예를 들어, 최대 3, 4, 5개, 또는 최대 10개)의 아미노산 잔기 결실, 삽입 및/또는 치환을 가질 수 있다. 일부 실시양태에서, 아미노산 서열은 선택적으로 1-2, 1-3, 1-4, 1-5, 1-6, 1-7, 1-8, 1-9, 1-10, 1-15, 1-20, 1-21, 1-22, 1-23, 1-24, 1-25, 1-30, 1-35, 1-40, 1-45 또는 1-50개의 아미노산 잔기 결실, 삽입 및/또는 치환을 갖는다. 일부 실시양태에서, 아미노산 서열은 선택적으로 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30, 30, 35, 40, 45, 또는 50개의 아미노산 잔기 결실, 삽입 및/또는 치환을 갖는다. 일부 실시양태에서, 아미노산 서열은 선택적으로 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 20, 21, 22, 23, 24, 또는 25개의 아미노산 잔기 결실, 삽입 및/또는 치환을 갖는다. 일부 실시양태에서, 치환은 보존적 또는 비보존적 치환이다.
발현된 조작된 LDC 폴리펩타이드는 본원에서 설명되는 검정 및 조건을 포함하지만 이에 제한되지 않는 관련 기술 분야에 공지된 임의의 적합한 분석을 사용하여 임의의 원하는 개선된 특성 또는 특성의 조합(예를 들어, 활성, 선택성, 안정성 등)에 대해 평가될 수 있다.
일부 실시양태에서, 숙주 세포에서 발현된 임의의 조작된 LDC 폴리펩타이드는 특히 리소자임 처리, 초음파 처리, 여과, 염석, 초원심분리 및 크로마토그래피를 포함하는 단백질 정제를 위한 잘 알려진 기술 중 하나 이상의 임의의 기술을 사용하여 세포 및/또는 배양 배지로부터 회수된다.
LDC 폴리펩타이드의 단리를 위한 크로마토그래피 기술은 특히 역상 크로마토그래피, 고성능 액체 크로마토그래피, 이온 교환 크로마토그래피, 소수성 상호작용 크로마토그래피, 크기 배제 크로마토그래피, 겔 전기영동 및 친화성 크로마토그래피를 포함한다. 특정 효소를 정제하기 위한 조건은 부분적으로 순 전하, 소수성, 친수성, 분자량, 분자 모양 등과 같은 인자에 의존하며, 이는 관련 기술 분야의 통상의 기술자에게 자명할 것이다. 일부 실시양태에서, 친화성 기술을 사용하여 개선된 LDC 효소를 단리할 수 있다. 친화성 크로마토그래피 정제를 위해, 관심 있는 LDC 폴리펩타이드에 특이적으로 결합하는 임의의 항체가 사용될 수 있다. 항체 생산을 위해, 토끼, 마우스, 래트 등을 포함하지만 이에 제한되지 않는 다양한 숙주 동물은 LDC 폴리펩타이드 또는 그의 단편으로 주사하여 면역화된다. 일부 실시양태에서, LDC 폴리펩타이드 또는 단편은 측쇄 작용기 또는 측쇄 작용기에 부착된 링커에 의해 BSA와 같은 적합한 담체에 부착된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 본원에서 설명되는 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열을 포함하는 숙주 세포(예를 들어, 이. 콜라이 세포주)를 조작된 LDC 폴리펩타이드의 생산을 유하는 조건 하에서 배양하는 단계 및 세포 및/또는 배양 배지로부터 조작된 LDC 폴리펩타이드를 회수하는 단계를 포함하는 방법에 의해 숙주 세포에서 생산된다. 일부 실시양태에서, 숙주 세포는 하나 초과의 조작된 LDC 폴리펩타이드를 생산한다.
일부 실시양태에서, 본 발명은 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766의 참조 서열에 대해 적어도 85%, 90%, 95%, 96%, 97%, 98%, 또는 99%의 서열 동일성, 및 본원에서 제공되는 바와 같이 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766과 비교하여 하나 이상의 아미노산 잔기 차이를 갖는 조작된 LDC 폴리펩타이드를 코딩하는 폴리뉴클레오타이드 서열을 포함하는 재조합 박테리아 세포를 조작된 LDC 폴리펩타이드의 생산을 허용하는 적합한 조건 하에서 배양하는 단계, 및 선택적으로 배양액 및/또는 배양된 박테리아 세포로부터 조작된 LDC 폴리펩타이드를 회수하는 단계를 포함하는, 조작된 LDC 폴리펩타이드를 생산하는 방법을 제공한다. 일부 실시양태에서, 숙주 세포는 하나 초과의 조작된 LDC 폴리펩타이드를 생산한다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드가 재조합 숙주 세포 및/또는 배양 배지로부터 회수되면, 이들은 관련 기술 분야에 공지된 임의의 적합한 방법(들)에 의해 추가로 정제된다. 추가의 일부 실시양태에서, 정제된 LDC 폴리펩타이드는 다른 성분 및 화합물과 조합되어 상이한 적용 및 용도(예를 들어, 약학 조성물)에 적합한 조작된 LDC 폴리펩타이드를 포함하는 조성물 및 제제를 제공한다.
조성물:
본 발명은 많은 조성물에 사용하기에 적합한 조작된 LDC 폴리펩타이드를 제공한다. 이들 조성물은 제약, 식이/영양 보충제, 식품, 사료 및 정밀 화학물질 생산을 포함하지만 이에 제한되지 않는 많은 분야에서 사용된다. 예를 들어, 일부 실시양태에서, 본 발명은 적어도 하나의 조작된 LDC 변이체 및/또는 적어도 하나의 LDC 변이체를 코딩하는 적어도 하나의 폴리뉴클레오타이드 서열을 포함하는 식품 및/또는 사료를 제공한다. 일부 실시양태에서, 본 발명은 적어도 하나의 조작된 LDC 변이체를 포함하는 음료를 제공한다.
일부 실시양태에서, 식품, 사료 및/또는 영양/식이 보충제 내의 조작된 LDC 변이체는 글리코실화된다. 또한, 조작된 LDC 변이체는 임의의 적합한 식용 효소 전달 매트릭스에 사용된다. 일부 실시양태에서, 조작된 LDC 변이체는 변이체 섭취시에 동물의 소화관 내에서 LDC 변이체의 신속한 분산을 위해 설계된 식용 효소 전달 매트릭스에 존재한다.
본 발명은 또한 정밀 화학물질 및 기타 산업적으로 중요한 화합물의 생산에 사용하기에 적합한 조작된 LDC 폴리펩타이드를 제공한다(예를 들어, 미국 특허 출원 공개 2013/0340119, 2013/0005012 및 2005/0260724, 및 WO 2012/122333 참조).
약학 조성물 및 기타 조성물:
본 발명은 약학 조성물 및 기타 조성물, 예를 들어 식이/영양 보충제에 사용하기에 적합한 조작된 LDC 폴리펩타이드를 제공한다.
투여 방식에 따라, 본 발명에 따른 조작된 LDC의 치료 유효량을 포함하는 이들 조성물은 고체, 반고체 또는 액체의 형태이다. 일부 실시양태에서, 조성물은 희석제, 완충제, 부형제, 염, 유화제, 보존제, 안정화제, 충전제 및 기타 성분과 같은 기타 약제학상 허용되는 성분을 포함한다. 제제화 및 투여를 위한 기술에 대한 세부사항은 관련 기술 분야에 잘 알려져 있고, 문헌에 기재되어 있다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 경구용 약학 조성물에 사용하기 위해 제제화된다. 환제, 정제, 겔 탭, 캡슐, 로젠지, 당의정, 분말, 연질 겔, 졸-겔, 겔, 에멀젼, 임플란트, 패치, 스프레이, 연고, 도포제, 크림, 페이스트, 젤리, 페인트, 에어로졸, 츄잉껌, 완화제, 스틱, 현탁액(유성 현탁액, 수중유 에멀젼 등을 포함하지만 이에 국한되지 않음), 슬러리, 시럽, 제어 방출 제제, 좌약 등을 포함하지만 이에 제한되지 않는, 조작된 LDC 폴리펩타이드를 전달하는데 사용하기 위한 임의의 적합한 형식이 본 발명에서 사용된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 주사에 적합한 형식(즉, 주사 가능한 제제)으로 제공된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 실리카 기반(예를 들어, 옥시실란) 졸-겔을 포함하는 졸-겔과 같은 생체적합성 매트릭스로 제공된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 캡슐화된다. 일부 대안적인 실시양태에서, 조작된 LDC 폴리펩타이드는 나노구조(예를 들어, 나노튜브, 나노세관, 나노캡슐, 또는 마이크로캡슐, 미소구체, 리포솜 등)로 캡슐화된다. 실제로, 본 발명은 임의의 특정 전달 제제 및/또는 전달 수단으로 제한되는 것으로 의도되지 않는다. 조작된 LDC 폴리펩타이드는 비경구, 경구, 국소, 경피, 비강내, 안내, 척추강내, 임플란트 등을 포함하지만 이에 제한되지 않는, 관련 기술 분야에 공지된 임의의 적합한 수단에 의해 투여되는 것으로 의도된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 글리코실화, 페길화(즉, 폴리에틸렌 글리콜[PEG] 또는 활성화된 PEG 등으로 변형됨) 또는 기타 화합물에 의해 화학적으로 변형된다(예를 들어, 문헌 [Ikeda, Amino Acids 29:283-287 [2005]}; 미국 특허 7,531,341, 7,534,595, 7,560,263, 및 7,553,653; US 특허 출원 공개 2013/0039898, 2012/0177722 등 참조). 실제로, 본 발명은 임의의 특정 전달 방법 및/또는 메커니즘으로 제한되는 것으로 의도되지 않는다.
추가의 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 기질-안정화 효소 결정을 포함하는 제제로 제공된다. 일부 실시양태에서, 제제는 가교결합된 결정질의 조작된 LDC 효소 및 효소 결정에 부착되는 반응성 모이어티를 갖는 중합체를 포함한다. 본 발명은 또한 중합체 내에서 조작된 LDC 폴리펩타이드를 제공한다.
일부 실시양태에서, 본 발명의 조작된 LDC 폴리펩타이드를 포함하는 조성물은 당(예를 들어, 락토스, 수크로스, 만니톨, 및/또는 소르비톨), 전분(예를 들어, 옥수수, 밀, 쌀, 감자 또는 기타 식물 전분), 셀룰로오스(예를 들어, 메틸 셀룰로오스, 하이드록시프로필메틸 셀룰로오스, 나트륨 카르복시-메틸셀룰로오스), 검(예를 들어, 아라비아, 트라가칸트, 구아 등) 및/또는 단백질(예를 들어, 젤라틴, 콜라겐 등)을 포함하지만 이에 제한되지 않는, 하나 이상의 통상적으로 사용되는 담체 화합물을 포함한다. 경구 제제의 추가의 성분은 착색제 및/또는 감미제(예를 들어, 글루코스, 수크로스 및 만니톨) 및 윤활제(예를 들어, 스테아르산마그네슘)뿐만 아니라, 장용 코팅(예를 들어, 메타크릴레이트 중합체, 하이드록실 프로필 메틸 셀룰로오스 프탈레이트 및 /또는 관련 기술 분야에 공지된 임의의 다른 적합한 장용 코팅)을 포함할 수 있다. 일부 실시양태에서, 붕해제 또는 가용화제(예를 들어, 가교결합된 폴리비닐 피롤리돈, 한천, 알긴산 또는 알긴산나트륨과 같은 그의 염)포함된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 특히 액체 제제에서 방부제, 현탁제, 증점제, 습윤제, 알코올, 지방산 및/또는 유화제를 포함하지만 이에 제한되지 않는 다양한 추가의 성분과 조합된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 특히 액체 제제에서 방부제, 현탁제, 증점제, 습윤제, 알코올, 지방산 및/또는 유화제를 포함하지만 이에 제한되지 않는 다양한 추가의 성분과 조합된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 MSUD의 치료에 사용되는 다른 화합물뿐만 아니라, 임의의 다른 적합한 화합물과 조합하여 대상체에게 투여된다.
일부 실시양태에서, 본 발명은 MSUD의 징후 및/또는 증상을 감소, 개선 또는 제거하기 위해 사용하기 적합한 조작된 LDC 폴리펩타이드를 제공한다. 환자에게 투여되는 조작된 LDC 폴리펩타이드(들)의 투여량은 환자의 유전자형, 환자의 일반적인 상태, 및 관련 기술 분야의 통상의 기술자에게 공지된 기타 인자에 의존한다. 일부 실시양태에서, 조성물은 환자에게 단일 또는 반복 투여를 위한 것이다. 일부 실시양태에서, 환자에게 투여되는 조성물(들) 내의 조작된 LDC 폴리펩타이드(들)의 농도는 질병의 증상을 효과적으로 치료, 개선 및/또는 예방하기에 충분한 것으로 고려된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 다른 약학 조성물 및/또는 식이 조성물과 조합하여 투여된다.
산업적 조성물:
본 발명의 조작된 LDC 폴리펩타이드는 식품 향료와 같은 영역(예를 들어, 치즈)을 포함하는 산업적 조성물에서 사용될 수 있을 것으로 고려된다.
일부 실시양태에서, 조작된 LDC 폴리펩타이드는 식품 및/또는 사료 산업에서 사용하기 위해 제제화된다. 일부 실시양태에서, 조작된 LDC 폴리펩타이드는 추가의 효소(예를 들어, 셀룰라아제, 라카아제 및 아밀라아제)와 같은 동물 사료 성분과 혼합되는 과립 또는 펠렛화된 제품으로 제제화된다. 일부 대안적인 실시양태에서, 조작된 LDC 폴리펩타이드는 액체 동물 사료 조성물(예를 들어, 수성 또는 유성 슬러리)에 사용된다. 따라서, 일부 실시양태에서, 본 발명의 조작된 LDC 변이체는 펠렛 및 기타 가공된 사료/식품을 생산하기 위해 사용되는 처리를 견디기에 충분히 내열성이고 열안정성이다.
본 발명의 상기 및 다른 측면은 하기 비제한적인 실시예와 관련하여 더 잘 이해될 수 있다. 실시예는 예시 목적으로만 제공되며, 어떤 식으로든 본 발명의 범위를 제한하도록 의도되지 않는다.
실험
달성된 실험 및 결과를 포함하는 하기 실시예는 예시 목적으로만 제공되며, 본 발명을 제한하는 것으로 해석되어서는 안 된다.
하기 실험 개시내용에서, 하기 약어가 적용된다: ppm(백만분율); M(몰); mM(밀리몰), uM 및 μM(마이크로몰); nM(나노몰); mol(몰); gm 및 g(그램); mg(밀리그램); ug 및 μg(마이크로그램); L 및 l(리터); ml 및 mL(밀리리터); cm(센티미터); mm(밀리미터); um 및 μm(마이크로미터); sec.(초); min(s)(분); h(s) 및 hr(s)(시); U(단위); MW(분자량); rpm(분당 회전수); psi 및 PSI(제곱인치당 파운드); ℃(섭씨 온도); RT 및 rt(실온); CDS(코딩 서열); DNA(데옥시리보핵산); RNA(리보핵산); AUC(곡선 아래 면적); 이. 콜라이 W3110(일반적으로 사용되는 실험실 이. 콜라이 세포주, 미국 코네티컷주 뉴 헤이븐 소재의 Coli Genetic Stock Center[CGSC]로부터 입수 가능); iMSUD(중등도 메이플 시럽 요증); HTP(고속: High-Throughput); HPLC(고압 액체 크로마토그래피); LC(액체 크로마토그래피); MS(질량 분광법); LC-MS/MS(2개의 질량 분석기를 사용한 액체 크로마토그래피); SPE(고상 추출); KIC(케토이소카프로에이트); IPTG(이소프로필 β-D-1-티오갈락토피라노사이드); PLP(피리독살 5'-포스페이트); BSA(소 혈청 알부민); BW(체중); MSUD(메이플 시럽 요증); FIOPC(양성 대조군에 비해 개선된 배수); LB(루리아 브로쓰(Luria broth)); TB(테리픽 브로쓰(Terrific broth)); 이노버티브 리써치(Innovative Research, 미국 미시건주 노비 소재); 마이크로플루이딕스(Microfluidics Corp., 미국 매사추세츠주 뉴튼 소재); 써모트론(Thermotron, 미국 미시건주 홀란드 소재); 워터스(Waters Corp., 미국 매사추세츠주 밀포드 소재); 인포르스(Infors AG, 스위스 보트밍겐 소재); 캠브리지 이소토프 래보러토리즈(Cambridge Isotope Laboratories, Inc., 미국 매사추세츠주 턱스베리 소재); 시그마-알드리치(Sigma-Aldrich, 미국 미주리주 세이트 루이스 소재); 어플라이드 바이오시스템즈(Applied Biosystems, Life Technologies, Corp.의 자회사, 미국 뉴욕주 그랜드 아일랜드 소재), 애질런트(Agilent Technologies, Inc., 미국 캘리포니아주 산타 클라라 소재); 써모 사이언티픽(Thermo Fisher Scientific의 자회사, 미국 매사추세츠주 월담 소재); 깁코(Gibco, Thermo Fisher Scientific의 자회사, 미국 매사추세츠주 월담 소재); 쿠너(Khuner Shaker, Inc., 미국 캘리포니아주 산타 클라라 소재); BN 랩스(British Nutritions, LLC, 미국 캘리포니아주 어빈 소재); 그래프패드 소프트웨어(GraphPad Software, 미국 캘리포니아주 샌디에고 소재); 및 코닝(Corning, Inc., 미국 캘리포니아주 팔로 알토 소재).
실시예 1
류신 데카르복실라제 활성을 갖는 아미노산 데카르복실라제 효소의
합성 및 분석
이 실시예에서, 류신 데카르복실라제(LDC) 활성에 대한 아미노산 데카르복실라제 효소의 합성 및 검정에 사용된 방법이 설명된다.
아미노산 데카르복실라제 유전자 획득 및 발현 벡터의 구축
스트렙토마이세스 종 GP55(서열 번호 2; Acc. No. WP_101384472.1), 사카로트릭스(Saccharothrix) 종 ST-888(서열 번호 4; Acc. No. WP_052681825.1), 및 키타사토스포라(Kitasatospora) 종 MBT63(서열 번호 6; Acc. No. WP_051812394.1), 키타사토스포라 종 MMS61-BH015(서열 번호 8; Acc. No. WP_104818078.1), 스트렙토마이세스 종 NRRL F-6131(서열 번호 10; Acc. No. WP_051769113.1), 플랑크토마이세타세아에 박테리아(서열 번호 12; Acc. No. RPI63066.1), 및 라르키넬라 아르보리콜라(Larkinella arboricola)(서열 번호 14; Acc. No. A0A327WPB0)로부터 얻은 아미노산 데카르복실라제를 코딩하는 폴리뉴클레오타이드를 각각 서열 번호 1, 3, 5, 7, 9, 11 및 13의 유전자로서 합성하였다. 이들 합성 유전자는 pCK110900 벡터 시스템 내로 클로닝되고(예를 들어, 둘 모두 본원에 참고로 포함된 미국 특허 제7,629,157호 및 미국 특허 출원 공개 2016/0244787 참조), 후속적으로 W3110으로부터 유래된 이. 콜라이 균주에서 발현되었다. 일부 실시양태에서, 항미생물 내성 마커가 결여된 발현 벡터가 사용된다.
진탕 플라스크 분말(SFP) 생산
아미노산 데카르복실라제를 함유하는 플라스미드로 형질전환된 이. 콜라이 배양액을 1% 글루코스 및 일부 경우에 30 ㎍/mL의 클로람페니콜이 포함된 루리아 브로쓰-한천 플레이트에 플레이팅하고, 37℃에서 밤새 성장시켰다. 각각의 배양액의 단일 콜로니를, 적절한 경우 1% 글루코스 및 30 μg/mL의 클로람페니콜이 포함된 5 mL의 루리아 브로쓰(LB)로 옮겼다. 배양액을 30℃, 250 rpm에서 18시간 동안 성장시키고, 30 μg/mL의 클로람페니콜이 포함된 250 ml의 테리픽 브로쓰(TB)에 대략 1:50으로 계대 배양하였다. 배양액을 30℃, 250 rpm에서 약 3-4시간 동안 0.6-0.8의 OD600까지 성장시키고, 1 mM의 IPTG로 유도하였다. 배양액을 30℃, 250 rpm에서 20시간 동안 성장시켰다. 세포를 원심분리(7000 rpm x 10분, 4℃)에 의해 수거하고, 상청액을 버렸다. 펠렛을 1 mM의 PLP와 함께 30 mL의 50 mM 인산나트륨(pH 7.0)에 재현탁하고, 110 psi에서 미세유동화기(Microfluidics)를 통한 단일 통과를 사용하여 용해시켰다. 용해물을 펠렛화하고(10,000 x rpm, 30분, 4℃), 생성된 상청액을 동결건조하여 발현된 효소를 함유하는 분말을 생성하였다.
상이한 pH에서 류신에 대한 데카르복실라제의 활성에 대한 SFP 특성화 검정
진탕 플라스크 분말을 재구성하여 40 g/L의 분말을 제공하고, 2.5-40 g/L로 연속 희석하였다. 그런 다음, 25 μL의 상기 스톡 용액을 50 mM 인산나트륨(pH 7.0) 또는 매킬베인(McIlvaine) 완충제(pH 4.6)에서 10 mM 류신 및 0.1 mM PLP의 최종 농도를 달성하기 위해 75 μL의 반응 혼합물에 첨가하였다. 반응물을 0.1% 포름산이 포함된 3부피의 아세토니트릴로 켄칭하기 전에 THERMOTRON® 역가 플레이트 진탕기에서 37℃, 250 rpm에서 3-4시간 동안 인큐베이션하였다. 생성된 샘플을 4℃에서 4000 rpm으로 10분 동안 원심분리한 후, 상청액을 류신의 탈카르복실화 생성물인 이소펜틸아민에 대해 LC-MS/MS에 의해 분석하였다. LC-MS/MS 기기 및 파라미터의 예는 표 1-1에 표시되어 있고, 서열 번호 2-14에 대한 결과는 표 1-2에 나와 있다.
Figure pct00057
pH 내성에 대한 SFP 특성화 검정
산성 pH에 대한 효소의 상대적 내성을 평가하기 위해, SFP를 재구성하여 20 g/L 분말을 제공하고, 매킬베인 완충제(pH 2.8-8.0)로 2배 희석하였다. 혼합물을 THERMOTRON® 역가 플레이트 진탕기에서 250 rpm으로 37℃에서 1.5시간 동안 인큐베이션하였다. 인큐베이션 후, 20 μL의 효소 용액을 50 mM 인산나트륨(pH 7.0) 중의 5 mM 류신의 최종 농도를 위해 90 μL의 반응 혼합물에 첨가하였다. 반응물은 THERMOTRON®에서 250 rpm으로 37℃에서 2시간 동안 인큐베이션한 후, 물에 2배 희석하고, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하였다. 생성된 샘플을 상기 기재된 바와 같이 처리하고, 상청액을 LC-MS/MS에 의해 이소펜틸아민에 대해 분석하였다. 서열 번호 2-14에 대해 얻은 결과는 표 1-2에 제시되어 있다.
열안정성에 대한 SFP 특성화 검정
개선된 열안정성은 효소 치료제의 제조 및 저장에 유용한 유용한 특성이며, 종종 다른 안정화 노력의 부산물로 수득된다. 본 발명의 개발 동안 생성된 변이체의 상대적 안정성을 평가하기 위해, 효소의 열안정성을 다음과 같이 평가하였다: 10 g/L의 아미노산 데카르복실라제 SFP 100 μL를 30-70℃에서 1.5시간 동안 열순환기에서 인큐베이션하였다. 인큐베이션 후, 샘플을 짧게 원심분리하고, 10 μL의 열처리된 SFP를 90 μL의 반응 혼합물에 첨가하여 50 mM 인산나트륨(pH 7.0) 내의 5 mM 류신의 최종 농도를 달성하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 2시간 동안 인큐베이션하고, 위에서 설명한 대로 처리하고, LC-MS/MS에 의해 분석하였다. 서열 번호 2-14에 대한 결과는 표 1-2에 제시되어 있다.
프로테아제에 대한 내성에 대한 SFP 특성화 검정
대표적인 장내 프로테아제에 대한 효소의 상대적 안정성을 평가하기 위해, 돼지 트립신(Sigma Aldrich) 및 소 키모트립신(Sigma Aldrich)의 혼합물을 50 mM 인산나트륨(pH 7.0)에 각각 3 g/L의 농도로 용해시키고, 2배씩 연속 희석하였다. 그 다음, 10 g/L의 아미노산 데카르복실라제 SFP를 THERMOTRON® 역가 플레이트 진탕기에서 0-1.5 g/L의 트립신/키모트립신과 함께 37℃에서 250 rpm으로 1시간 동안 인큐베이션하였다. 인큐베이션 후, 10 μL의 프로테아제 처리된 SFP를 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신의 최종 농도를 위해 90 μL의 반응 혼합물에 첨가하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 1시간 동안 인큐베이션한 후, 물에 5배 희석하고, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하였다. 생성된 샘플을 상기 기재된 바와 같이 처리하고, 상청액을 상기 기재된 바와 같이 LC-MS/MS에 의해 분석하였다. 서열 번호 2 및 12에 대한 결과는 표 1-2에 제시되어 있다.
Figure pct00058
실시예 2
서열 번호 12의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 12로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 11에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
LDC 효소를 발현하는 배양액의 고속(HTP) 성장
형질전환된 이. 콜라이 세포를 1% 글루코스를 함유하는 LB 한천 플레이트 상에 플레이팅함으로써 선택하였다. 37℃에서 밤새 인큐베이션한 후, 콜로니를 1% 글루코스가 보충된 180 μL/웰의 LB 배지로 채워진 NUNC™(Thermo-Scientific)의 얕고 평평한 바닥의 96웰 플레이트에 집어넣었다. 배양액을 쿠너 진탕기(200 rpm, 30℃ 및 85% 상대 습도)에서 18-20시간 동안 밤새 성장하도록 두었다. 밤새 성장한 샘플(20 μL)을 380 μL의 TB로 채워진 COSTAR®의 96웰 딥 플레이트(Corning)로 옮겼다. 배양액을 쿠너 진탕기(250 rpm, 30℃ 및 85% 상대 습도)에서 2-3시간 동안 인큐베이션한 다음, 멸균수 내의 40 μL의 10 mM IPTG로 유도하고, 쿠너 진탕기(250 rpm, 30℃ 및 85% 상대 습도)에서 20-24시간 동안 밤새 인큐베이션하였다. 세포를 펠렛화하고(4000 rpm x 10분), 상청액을 버리고, 세포를 분석 전에 -80℃에서 동결시켰다.
HTP 세포 펠렛의 용해
이. 콜라이 세포 펠렛을 400 μL의 용해 완충제(50 mM 인산나트륨(pH 7) 중의 1 mg/ml 리소자임 + 0.5 g/L PMBS)로 용해하였다. 혼합물을 실온에서 1.5시간 동안 교반하고, 펠렛화(4000 rpm x 10분)한 후, 정화된 용해물을 THERMOTRON® 역가 플레이트 진탕기(400 rpm)에서 60℃에서 1시간 동안 사전 인큐베이션하였다. 열처리된 용해물을 펠렛화하고(4000 rpm x 10분), 상청액을 HTP 검정에 사용하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 함유하는 열처리된 용해물을 위 환경을 시뮬레이션하기 위해 산성 완충제로 챌린지하였다. 먼저, 열처리된 정화된 용해물을 COSTAR® 96웰 둥근 바닥 플레이트(Corning)에서 매킬베인 완충제(pH 3.6)와 함께 1:1로 사전 인큐베이션하였다. 플레이트를 밀봉하고, THERMOTRON® 역가 플레이트 진탕기(250 rpm)에서 37℃에서 1시간 동안 인큐베이션하였다. 이어서, 생성된 산성 완충제 처리된 용해물을 진탕하면서 최종 농도 0.02 g/L의 트립신 및 키모트립신(1:1)과 함께 1:1로 37℃에서 1시간 동안 인큐베이션하였다. 인큐베이션 후, 생성된 샘플을 원심분리하고, 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신 및 10 μM PLP의 최종 농도를 위해 50 μL의 샘플을 50 μL의 반응 혼합물에 첨가하였다. 일부 실험에서, 이소류신 대신 이소류신-d10(Cambridge Isotope Laboratories)을 사용하고 50 mM 인산나트륨(pH 7.0) 내의 10 μM PLP를 사용하여 20개의 모든 아미노산의 최종 농도가 0.6-3 mM인 반응 혼합물을 사용하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 1시간 동안 인큐베이션한 후, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하고, 4℃에서 4000 rpm으로 10분 동안 원심분리하고, 물에 50배 희석하였다. 생성된 샘플을 실시예 1에서 설명된 바와 같이 LC-MS/MS에 의해 분석하였다. 이러한 검정의 결과는 표 2-1에 제시되어 있다.
Figure pct00059
Figure pct00060
Figure pct00061
Figure pct00062
실시예 3
서열 번호 38의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 38로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 38에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해를 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 실시예 2에 기재된 바와 같이 산성 완충제로 챌린지하였다. 이어서, 산성 완충제 처리된 용해물을 진탕하면서 최종 농도 0.1 g/L의 트립신 및 키모트립신(1:1)의 최종 농도로 1:1로 37℃에서 1시간 동안 인큐베이션하였다. 인큐베이션 후, 샘플을 원심분리하고, 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신 및 10 μM PLP의 최종 농도를 위해 50 μL의 샘플을 50 μL의 반응 혼합물에 첨가하였다. 일부 실험에서, 이소류신 대신 이소류신-d10(Cambridge Isotope Laboratories)을 사용하고 50 mM 인산나트륨(pH 7.0) 내의 10 μM PLP를 사용하여 20개의 모든 아미노산의 최종 농도가 0.6-3 mM인 반응 혼합물을 사용하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 1시간 동안 인큐베이션한 후, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하고, 4℃에서 4000 rpm으로 10분 동안 원심분리하고, 물에 50배 희석하였다. 생성된 샘플을 실시예 1에서 설명된 바와 같이 LC-MS/MS에 의해 또는 표 3-1에 제시된 파라미터를 사용하여 래피드파이어® 질량 분석법(Agilent)에 의해 분석하였다. 이러한 검정의 결과는 표 3-2에 제시되어 있다.
Figure pct00063
Figure pct00064
Figure pct00065
Figure pct00066
실시예 4
서열 번호 234의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 234로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 234에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해를 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 다음 조건을 사용하여 실시예 2에 기재된 바와 같이 산성 완충제로 챌린지하였다: 열처리된 정화된 용해물을 매킬베인 완충제(pH 3.3)와 함께 1:1로 사전 인큐베이션하였다. 이어서, 생성된 산성 완충제 처리된 용해물을 진탕하면서 최종 농도 0.5 g/L의 트립신 및 키모트립신(1:1)의 최종 농도로 1:1로 37℃에서 1시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 샘플을 원심분리하고, 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신 및 10 μM PLP의 최종 농도를 위해 40 μL의 샘플을 60 μL의 반응 혼합물에 첨가하였다. 일부 실험에서, 이소류신 대신 이소류신-d10(Cambridge Isotope Laboratories)을 사용하고 50 mM 인산나트륨(pH 7.0) 내의 10 μM PLP를 사용하여 20개의 모든 아미노산의 최종 농도가 0.6-3 mM인 반응 혼합물을 사용하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 1시간 동안 인큐베이션한 후, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하고, 4℃에서 4000 rpm으로 10분 동안 원심분리하고, 물에 50배 또는 500배 희석하였다. 생성된 샘플을 실시예 1에서 설명된 바와 같이 LC-MS/MS에 의해 또는 실시예 3에서 설명된 바와 같이 래피드파이어®-MS에 의해 분석하였다.
Figure pct00067
Figure pct00068
Figure pct00069
실시예 5
서열 번호 284의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 284로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 284에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제, 펩신 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해를 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 위 환경을 시뮬레이션하기 위해 펩신을 함유하는 산성 완충제로 챌린지하였다. 먼저, 열처리된 정화된 용해물을 COSTAR® 96웰 둥근 바닥 플레이트(Corning)에서 돼지 위 점막(Sigma)으로부터의 0.05 g/L 펩신을 함유하는 매킬베인 완충제(pH 3.2)와 1:1로 사전 인큐베이션하였다. 플레이트를 밀봉하고, THERMOTRON® 역가 플레이트 진탕기(250 rpm)에서 37℃에서 1시간 동안 인큐베이션하였다. 이어서, 샘플을 짧게 원심분리하고, 생성된 상청액을 진탕하면서 최종 농도 0.5 g/L의 트립신 및 키모트립신(1:1)과 함께 1:1로 37℃에서 1시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신 및 10 μM PLP의 최종 농도를 위해 40 μL의 샘플을 60 μL의 반응 혼합물에 첨가하였다. 반응물은 THERMOTRON® 역가 플레이트에서 250 rpm으로 37℃에서 1시간 동안 인큐베이션한 후, 0.1% 포름산이 포함된 3 부피의 아세토니트릴로 켄칭하고, 4℃에서 4000 rpm으로 10분 동안 원심분리하고, 물에 500배 희석하였다. 생성된 샘플을 실시예 3에서 설명된 바와 같이 래피드파이어®-MS에 의해 분석하였다.
Figure pct00070
Figure pct00071
실시예 6
서열 번호 484의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 484로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 484에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해는 용해 완충제가 40 μM PLP를 함유한 것을 제외하고는 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 펩신을 함유하는 산성 완충제로 챌린지한 후, 실시예 5에 기재된 바와 같이 프로테아제로 챌린지하였다. 구체적으로, 열처리된 정화된 용해물을 매킬베인 완충제(pH 3) 내의 0.375 g/L 펩신의 최종 농도로 37℃에서 1시간 동안 1:1로 사전 인큐베이션하고, 생성된 상청액을 200 mM 인산나트륨(pH 8)에 용해된 최종 농도 0.75 g/L의 트립신 및 키모트립신(1:1)과 함께 1:1로 37℃에서 1시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 50 mM 인산나트륨(pH 7.0) 내의 3 mM 류신의 최종 농도를 위해 40 μL의 샘플을 60 μL의 반응 혼합물에 첨가하였다. 반응물은 샘플을 물에 500배 희석한 후 래피드파이어®-MS에 의해 분석한 것을 제외하고는 실시예 5에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 분석하였다. 이들 검정의 결과는 표 6-1에 제시되어 있다.
Figure pct00072
Figure pct00073
Figure pct00074
실시예 7
서열 번호 594의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 594로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 594에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해는 용해 완충제가 40 μM PLP를 함유한 것을 제외하고는 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 펩신을 함유하는 산성 완충제로 챌린지한 후, 실시예 5에 기재된 바와 같이 프로테아제로 챌린지하였다. 구체적으로, 열처리된 정화된 용해물을 매킬베인 완충제(pH 2.8) 내의 0.2 g/L 펩신의 최종 농도로 37℃에서 1시간 동안 1:1로 사전 인큐베이션하고, 생성된 상청액을 400 mM 인산나트륨(pH 8)에 용해된 최종 농도 1.5 g/L의 트립신 및 키모트립신(1:1)과 함께 1:1로 37℃에서 1시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 50 mM 인산나트륨(pH 7.0) 내의 2.5 mM 류신의 최종 농도를 위해 40 μL의 샘플을 60 μL의 반응 혼합물에 첨가하였다. 반응물을 실시예 6에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 희석하고, 분석하였다. 이들 검정의 결과는 표 7-1에 제시되어 있다.
Figure pct00075
Figure pct00076
실시예 8
서열 번호 686의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 686으로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 686에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해는 용해 완충제가 40 μM PLP를 함유한 것을 제외하고는 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 펩신을 함유하는 산성 완충제로 챌린지한 후, 실시예 5에 기재된 바와 같이 프로테아제로 챌린지하였다. 구체적으로, 열처리된 정화된 용해물을 매킬베인 완충제(pH 2.8) 내의 0.4 g/L 펩신의 최종 농도로 37℃에서 1-1.5시간 동안 1:1로 사전 인큐베이션하고, 생성된 상청액을 400 mM 인산나트륨(pH 8)에 용해된 최종 농도 2-4 g/L의 트립신 및 1.5 g/L의 키모트립신(1:1)과 함께 1:1로 37℃에서 1-2시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 50 mM 인산나트륨(pH 7.0) 내의 2.5 mM 류신의 최종 농도를 위해 40 μL의 샘플을 60 μL의 반응 혼합물에 첨가하였다. 일부 실험에서, 50 mM 인산나트륨(pH 7.0) 내의 2.5 mM 류신, 이소류신-d10, 발린, 아스파라긴, 메티오닌 및 시스테인의 최종 농도를 생성하는 반응 혼합물을 사용하였다. 반응물을 실시예 6에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 희석하고, 분석하였다. 이들 검정의 결과는 표 8-1 및 8-2에 제시되어 있다.
Figure pct00077
Figure pct00078
실시예 9
LDC 변이체의 아미노산 활성 분석
이 실시예에서, 20개의 모든 아미노산에 대한 서열 번호 12 및 594의 활성을 평가하기 위한 실험이 기술된다.
진탕 플라스크 분말(SFP)의 생산
LDC 변이체의 진탕 플라스크 분말을 실시예 1에 기재된 바와 같이 제조하되, 다음과 같은 변화를 주었다: 세포 펠렛을 200 μM PLP가 포함된 50 mM 인산나트륨(pH 7.0) 30 mL에 재현탁시켰다. 110 psi에서 미세유동화기(Microfluidics)를 통한 단일 통과를 사용하여 용해시킨 후, 용해물을 펠렛화하고(10,000 x rpm, 30분, 4℃), 생성된 상청액을 수조에서 60℃에서 1시간 동안 열처리하였다. 그런 다음, 샘플을 원심분리(10,000 x rpm, 1시간, 4℃)한 후, 생성된 상청액을 동결하고, 동결건조하여 발현된 효소를 함유하는 분말을 생성하였다.
상이한 아미노산에 대한 데카르복실라제 활성에 대한 SFP 특성화 검정
진탕 플라스크 분말을 포스페이트 완충 식염수(pH 7.4)에서 재구성하여 10 g/L 분말의 스톡 용액을 제공하였다. 그런 다음, 20 μL의 상기 SFP 용액을 이소류신 대신 이소류신-d10(Cambridge Isotope Laboratories)을 사용하고 50 mM 인산나트륨 완충제(pH 7.0)에 용해된 2 mM의 20개의 모든 아미노산을 함유하는 180 μL의 반응 혼합물에 첨가하였다. 반응물은 THERMOTRON® 역가 플레이트 진탕기에서 400 rpm으로 37℃에서 1.5-2시간 동안 인큐베이션한 후, 0.1% 포름산이 포함된 2 부피의 아세토니트릴로 켄칭하였다. 생성된 샘플을 4℃에서 4000 rpm으로 10분 동안 원심분리한 후, 상청액을 0.1% 포름산이 포함된 아세토니트릴에 6배 희석하였다. 샘플을 LC-MS/MS에 의해 20개의 모든 아미노산에 대해 분석하였고, LC-MS/MS 기기 및 파라미터의 예는 표 9-1 및 9-2에 표시되어 있다. 각각의 아미노산의 전환(즉, 고갈)은 음성 대조군에 대해 계산하였고, 서열 번호 12 및 594에 대한 결과는 표 9-3에 제시되어 있다.
Figure pct00079
Figure pct00080
Figure pct00081
Figure pct00082
실시예 10
서열 번호 688의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 688으로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 688에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해는 용해 완충제가 20 mM 인산나트륨 및 40 μM PLP를 함유한 것을 제외하고는 실시예 2에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 펩신을 함유하는 산성 완충제로 챌린지한 후, 실시예 5에 기재된 바와 같이 프로테아제로 챌린지하였다. 구체적으로, 열처리된 정화된 용해물을 매킬베인 완충제(pH 3) 내의 0.4 g/L 펩신의 최종 농도로 37℃에서 1.5시간 동안 1:1로 사전 인큐베이션하고, 생성된 상청액을 400 mM 인산나트륨(pH 8)에 용해된 최종 농도 4 g/L의 트립신 및 1.5 g/L의 키모트립신(1:1)과 함께 1:1로 37℃에서 2시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 40 μL의 샘플을 시뮬레이션된 장액 내의 2.5 mM 류신, 이소류신-d10, 발린, 아스파라긴, 메티오닌 및 시스테인의 최종 농도를 생성하는 60 μL의 반응 혼합물에 첨가하였다. 반응물을 실시예 6에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 희석하고, 분석하였다. 이들 검정의 결과는 표 10-1에 제시되어 있다.
Figure pct00083
실시예 11
서열 번호 766의 LDC 변이체
이 실시예에서, 개선된 류신 활성, 낮은 pH 내성 및 프로테아제 저항성을 위해, 서열 번호 766으로부터 유래된 LDC 변이체의 진화 및 스크리닝에 대한 실험이 설명된다. 서열 번호 766에 의해 코딩되는 LDC의 유도 진화는 변이체 유전자의 라이브러리를 구축함으로써 수행되었다. 그런 다음, 이들 라이브러리를 아래에서 설명되는 방법을 사용하여 플레이팅하고, 성장시키고, 스크리닝하였다.
산성 완충제 및 프로테아제로 전처리된 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해는 실시예 10에 기재된 바와 같이 수행하였다. LDC 변이체를 함유하는 열처리된 용해물을 펩신을 함유하는 산성 완충제로 챌린지한 후, 실시예 5에 기재된 바와 같이 프로테아제로 챌린지하였다. 구체적으로, 열처리된 정화된 용해물을 매킬베인 완충제(pH 2.6-2.8) 내의 0.8 g/L 펩신의 최종 농도로 37℃에서 2시간 동안 1:1로 사전 인큐베이션하고, 생성된 상청액을 400 mM 인산나트륨(pH 8)에 용해된 최종 농도 4 g/L의 트립신 및 1.5 g/L의 키모트립신과 함께 1:1로 37℃에서 2시간 동안 사전 인큐베이션하였다. 인큐베이션 후, 40 μL의 샘플을 시뮬레이션된 장액 내의 2-3 mM 류신, 이소류신-d10, 발린, 아스파라긴, 메티오닌 및 시스테인의 최종 농도를 생성하는 60 μL의 반응 혼합물에 첨가하였다. 반응물을 실시예 6에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 희석하고, 분석하였다. 이들 검정의 결과는 표 11-1 및 11-2에 제시되어 있다.
류신에 대한 정화된 용해물의 HTP 활성 분석
LDC 변이체를 발현하는 이. 콜라이 세포의 HTP 성장 및 용해를 실시예 10에 기재된 바와 같이 수행하였다. 열처리된 용해물을 물에 20배 희석하고, 20 μL의 샘플을 50 mM 인산나트륨(pH 7) 내의 3 mM 류신의 최종 농도를 생성하는 80 μL의 반응 혼합물에 첨가하였다. 반응물을 실시예 6에 기재된 바와 같이 인큐베이션하고, 켄칭하고, 희석하고, 분석하였다. 류신에 대한 활성은 SDS-PAGE 및 크기 배제 크로마토그래피에 의해 측정된, 열처리된 정화된 용해물 내의 류신 데카르복실라제의 농도에 의해 정규화되었다. 이러한 분석의 결과는 표 11-2에 제시되어 있다.
Figure pct00084
Figure pct00085
실시예 12
건강한 사이노몰구스 원숭이에서 LDC 변이체의 생체내 특성화
3개의 LDC 변이체(서열 번호 484; 서열 번호 686; 및 서열 번호 766)를 특성화하기 위해 건강한 사이노몰구스 원숭이(n=12, 수컷, 3-4 kg BW)에서 생체내 약력학 연구를 수행하였다. 각각의 변이체는 25, 50 또는 100 mg/kg으로 투여되었다. 연구는 각각 1주일의 휴약 기간으로 분리된 4일 투여로 구성된 4주에 걸쳐 수행되었다. 동물에게 동일한 용량을 두 번 투여하지 않도록 투여 그룹을 순환시켰다. 투여 하루 전에, 동물을 개별 케이지 내로 분리하고, 밤새 금식시켰다. 각각의 투여일에, 10 g의 유청 단백질 분말(BN Labs Grass Fed Whey Protein; 9.14% 류신)을 물과 함께 동물당 총 부피 20 mL로 제제화하여 경구 위관 영양법으로 공급하였다. 유청 단백질 현탁액을 공급한 직후에, 비히클(20 mM 인산나트륨, 0.4 mM 피리독살 포스페이트(pH 7.2) 또는 적절한 용량의 LDC를 2.5 mL/kg으로 제공한 다음, 동일한 위관을 사용하여 모든 물질의 효율적인 전달을 보장하기 위해 4 mL의 물로 세정하였다. 동물에게 8시간에, 채혈 직후에, 투여일에 정상적인 먹이를 제공하였다. 혈액 샘플은 식전 90분 및 30분에, 및 식후 5, 15, 30분 및 식후 1, 2, 4, 8, 12 및 24시간에 채취하였다(24시간 시점은 다음 날, 먹이 공급 전에 수집되었다). 샘플을 항응고제인 K2EDTA를 함유하는 튜브로 옮기고, 젖은 얼음 위에 놓고, 대략 4℃에서 10분 동안 2500 rpm으로 원심분리하였다. 생성된 혈장을 회수하여 분석할 때까지 냉동 보관(≤-60℃)하였다. 혈장 류신, 페닐알라닌, 티로신 및 메티오닌을 LC-MS/MS를 사용하여 정량하여 효능을 평가하였다. 식전 기준선과 비교하여 식사 챌린지 단독시에 혈장 류신의 유의한 증가가 관찰되었다. 모든 용량에서 모든 LDC 변이체는 유청 단백질 식사 챌린지에 대한 반응으로 혈장 류신의 상당한 억제를 초래하였다. 서열 번호 484 및 서열 번호 686은 용량 반응성 방식으로 효능을 입증하였다. 서열 번호 766의 모든 용량은 서열 번호 484 및 서열 번호 686 둘 모두보다 우수한 유사한 효능을 나타냈다. LDC는 혈장 페닐알라닌, 티로신 또는 메티오닌에 영향을 미치지 않았다. 표 12-1은 비히클과 비교하여 치료에 따른 혈장 류신 곡선 아래 증분 면적(iAUC)의 감소 백분율을 요약한 것이다. iAUC는 먼저 각각의 동물에 대한 각각의 식후 시점 값으로부터 식전 류신 값(금식 배경)을 뺀 다음, AUC 계산을 수행함으로써 기록하였다. 통계적 계산 및 유의성은 그래프패드 프리즘(GraphPad Prism) 7(GraphPad Software)을 사용하여 결정되었다.
Figure pct00086
실시예 13
마우스 질병 모델에서 LDC 변이체의 생체내 특성화
생체내 약력학 연구는 3개의 LDC 변이체(서열 번호 484, 서열 번호 686, 및 서열 번호 766)를 특성화하기 위해 중등도 메이플 시럽 요증(iMSUD; (Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J; JAX 스톡 #6999)) 마우스 모델에서 수행되었다. 각각의 변이체는 200 mg/kg으로 투여되었다. 투여 전 및 이유식 시작시에, iMSUD 마우스는 성장을 지원하고 생존을 연장하기 위해 음용수에 보충된 류신(5.75 g의 류신/L)을 공급하면서 류신 미함유 마우스 먹이로 유지하였다. 적어도 2개월령의 BW 20 g의 동물을 실험에 사용하였다. 동물을 투여 전에 밤새(~15시간) 금식시켰다. 각각의 투여일에, 45 mg의 유청 단백질 분말(BN Labs Grass Fed Whey Protein; 9.14% 류신)을 물과 함께 동물당 총 부피 100 mL로 제제화하여 경구 위관 영양법으로 공급하였다. 유청 단백질 현탁액을 공급한 직후에, 비히클(20 mM 인산나트륨, 0.4 mM 피리독살 포스페이트(pH 7.2) 또는 적절한 LDC 변이체를 5 mL/kg으로 제공하였다. 혈액 샘플은 식전 15분에, 및 식후 15, 30, 60, 120 및 240분에 채취하였다. 샘플을 항응고제인 K2EDTA를 함유하는 튜브로 옮기고, 젖은 얼음 위에 놓고, 대략 4℃에서 10분 동안 2500 rpm으로 원심분리하였다. 생성된 혈장을 회수하여 분석할 때까지 냉동 보관(≤-60℃)하였다. 혈장 류신을 LC-MS/MS를 사용하여 정량하여 효능을 평가하였다. 식전 기준선과 비교하여 식사 챌린지 단독시에 혈장 류신의 유의한 증가가 관찰되었다. 모든 LDC 변이체는 유청 단백질 식사 챌린지에 대한 반응으로 혈장 류신의 상당한 억제를 초래하였다. 서열 번호 484 및 서열 번호 686은 유사한 효능을 나타낸 반면, 서열 번호 766은 우수한 유사한 효능을 나타냈다. 표 13-1은 비히클과 비교하여 치료에 따른 혈장 류신 iAUC의 감소 백분율을 요약한 것이다. iAUC는 먼저 각각의 동물에 대한 각각의 식후 시점 값으로부터 식전 류신 값(금식 배경)을 뺀 다음, AUC 계산을 수행함으로써 기록하였다. 통계적 계산 및 유의성은 그래프패드 프리즘 7(GraphPad Software)을 사용하여 결정되었다.
Figure pct00087
본 발명이 특정 실시양태를 참조하여 설명되었지만, 특정 상황, 재료, 물질의 조성, 공정, 공정 단계 또는 단계들에 적응하기 위해 다양한 변경이 이루어질 수 있고, 등가물이 대체될 수 있으며, 이에 의해 특허 청구된 범위를 벗어나지 않으면서 본 발명의 이점을 달성할 수 있다.
미국에서의 모든 목적을 위해, 본 개시내용에 인용된 각각의 모든 간행물 및 특허 문서는 마치 각각의 그러한 간행물 또는 문서가 구체적이고 개별적으로 본 명세서에 참조로 포함되는 것으로 표시된 것처럼 본 명세서에 참조로 포함된다. 간행물 및 특허 문서의 인용은 그러한 문서가 관련 선행 기술이라는 표시로 의도되지 않았으며, 그 내용 또는 날짜에 대해 승인하는 것도 아니다.
SEQUENCE LISTING <110> CODEXIS, INC. <120> ENGINEERED LEUCINE DECARBOXYLASES <130> CX7-198WO2 <140> <141> 2021-02-03 <150> US 62/970,039 <151> 2020-02-04 <160> 852 <210> 1 <211> 1701 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Streptomyces sp. GP55 <400> 1 atgagcattt catcttctcc gctggatatc ttagcggcgg cctccgcagt ggacagcggc 60 ccagccgttg atccgggtcc tgaactggat tgccgcgact ttgagcttcc ggcttcgggg 120 ttggatgatg cacgtcgtct gcatgtcctg gataccgtgg acgaatatct gacgcgtaaa 180 cgtcgccacc tggttggtta ccaggctacc caagacatgc agggctgtgc gctcgattta 240 gcacgtttca tgcccaacaa catcaataat cttggcgatc cgtttcagag tggaggatat 300 aaaccaaaca ctaaggtagt agaacgcgcc gtgctggact actacgcgaa attatggcgc 360 gctaatggtc cgcatgatcc tgcggatccg gagagctatt ggggctatat gttgtcgatg 420 ggtagtacag aaggcaacat gtatgccctc tggaatgcac gcgactacct ctccgggaaa 480 gcgctgattc aaccacccac cgccccgttc gaagcgctgc gctatgtcaa agcacgtccg 540 gatcgcagca accctaatgc tcaccgtccg gtggccttct actcagaaga cacgcattat 600 tcgtttgcga aggcagttcg cgtcctgggt gtggagacct ttcacgctgt tggctgggaa 660 ttgtacccgg atgaatgccc actggtggat ccggccactg gccgtcgtga gtggcccatt 720 gaagtaccga gttctcctgg tccgtcaggt acgtcctggg atgggccagg cgaaatcgac 780 gtcgatagcc tggcggtgct ggtcgagttc tttgcagcga aaggtcatcc gatttttgtg 840 aaccttaact tgggaagcac cttcaaaggc gcccatgacg atgttcgcgg cgtttgtgaa 900 cgcctgctgc cgatctttga agaacacggg ctcattcagc gtgaggtggt gtatggtaca 960 gatcgtcgta ccggcgaacc tttagtagac ttacgccgcg gtttctggat ccatgttgat 1020 ggcgcccttg gtgcgggata tgcgccattt atgcgcatgg ctagtcagga cccggcagca 1080 cacggttgga cgcccgatgt cgagttgccg gaatacgatt ttggcttaac ccttccgact 1140 cgtgaattcg gtgacctcga tatggtgtcg tcgattgcca tgagcggcca taaatggcct 1200 ggcgccccat ggccttgcgg gatttatatg acgaaggtta aataccaaat ctctccgccg 1260 tcccagccgg actataccgg tgctccagat acaacatttg ccgggagccg taacggtttc 1320 tctccgttgg tcatttggga tcacctttca cgccatagtt accaggatca agtagatcgt 1380 atccgtgacg cacaggcctt ggctgactat ctcgaatgtc gcttacgtgc aatggaagga 1440 gagttaggcg tggaattgtg gccagctcgt actccgggtg ccgttaccgt gcgctttcgc 1500 aaacctagcg cagaactcgt agccaaatgg tcacttagct cccaagacgt cttaatggtt 1560 ccgggcgatg agatgacccg tcgttcgtac gtgcatgtct tcttgatgcc cagtgttgat 1620 cgcgcaaaat tggatgccct ccttgctgaa ttagctgaag atccggcaat tcgcggttta 1680 cccgcccgtt ctgcataata g 1701 <210> 2 <211> 565 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Streptomyces sp. GP55 <400> 2 Met Ser Ile Ser Ser Ser Pro Leu Asp Ile Leu Ala Ala Ala Ser Ala 1 5 10 15 Val Asp Ser Gly Pro Ala Val Asp Pro Gly Pro Glu Leu Asp Cys Arg 20 25 30 Asp Phe Glu Leu Pro Ala Ser Gly Leu Asp Asp Ala Arg Arg Leu His 35 40 45 Val Leu Asp Thr Val Asp Glu Tyr Leu Thr Arg Lys Arg Arg His Leu 50 55 60 Val Gly Tyr Gln Ala Thr Gln Asp Met Gln Gly Cys Ala Leu Asp Leu 65 70 75 80 Ala Arg Phe Met Pro Asn Asn Ile Asn Asn Leu Gly Asp Pro Phe Gln 85 90 95 Ser Gly Gly Tyr Lys Pro Asn Thr Lys Val Val Glu Arg Ala Val Leu 100 105 110 Asp Tyr Tyr Ala Lys Leu Trp Arg Ala Asn Gly Pro His Asp Pro Ala 115 120 125 Asp Pro Glu Ser Tyr Trp Gly Tyr Met Leu Ser Met Gly Ser Thr Glu 130 135 140 Gly Asn Met Tyr Ala Leu Trp Asn Ala Arg Asp Tyr Leu Ser Gly Lys 145 150 155 160 Ala Leu Ile Gln Pro Pro Thr Ala Pro Phe Glu Ala Leu Arg Tyr Val 165 170 175 Lys Ala Arg Pro Asp Arg Ser Asn Pro Asn Ala His Arg Pro Val Ala 180 185 190 Phe Tyr Ser Glu Asp Thr His Tyr Ser Phe Ala Lys Ala Val Arg Val 195 200 205 Leu Gly Val Glu Thr Phe His Ala Val Gly Trp Glu Leu Tyr Pro Asp 210 215 220 Glu Cys Pro Leu Val Asp Pro Ala Thr Gly Arg Arg Glu Trp Pro Ile 225 230 235 240 Glu Val Pro Ser Ser Pro Gly Pro Ser Gly Thr Ser Trp Asp Gly Pro 245 250 255 Gly Glu Ile Asp Val Asp Ser Leu Ala Val Leu Val Glu Phe Phe Ala 260 265 270 Ala Lys Gly His Pro Ile Phe Val Asn Leu Asn Leu Gly Ser Thr Phe 275 280 285 Lys Gly Ala His Asp Asp Val Arg Gly Val Cys Glu Arg Leu Leu Pro 290 295 300 Ile Phe Glu Glu His Gly Leu Ile Gln Arg Glu Val Val Tyr Gly Thr 305 310 315 320 Asp Arg Arg Thr Gly Glu Pro Leu Val Asp Leu Arg Arg Gly Phe Trp 325 330 335 Ile His Val Asp Gly Ala Leu Gly Ala Gly Tyr Ala Pro Phe Met Arg 340 345 350 Met Ala Ser Gln Asp Pro Ala Ala His Gly Trp Thr Pro Asp Val Glu 355 360 365 Leu Pro Glu Tyr Asp Phe Gly Leu Thr Leu Pro Thr Arg Glu Phe Gly 370 375 380 Asp Leu Asp Met Val Ser Ser Ile Ala Met Ser Gly His Lys Trp Pro 385 390 395 400 Gly Ala Pro Trp Pro Cys Gly Ile Tyr Met Thr Lys Val Lys Tyr Gln 405 410 415 Ile Ser Pro Pro Ser Gln Pro Asp Tyr Thr Gly Ala Pro Asp Thr Thr 420 425 430 Phe Ala Gly Ser Arg Asn Gly Phe Ser Pro Leu Val Ile Trp Asp His 435 440 445 Leu Ser Arg His Ser Tyr Gln Asp Gln Val Asp Arg Ile Arg Asp Ala 450 455 460 Gln Ala Leu Ala Asp Tyr Leu Glu Cys Arg Leu Arg Ala Met Glu Gly 465 470 475 480 Glu Leu Gly Val Glu Leu Trp Pro Ala Arg Thr Pro Gly Ala Val Thr 485 490 495 Val Arg Phe Arg Lys Pro Ser Ala Glu Leu Val Ala Lys Trp Ser Leu 500 505 510 Ser Ser Gln Asp Val Leu Met Val Pro Gly Asp Glu Met Thr Arg Arg 515 520 525 Ser Tyr Val His Val Phe Leu Met Pro Ser Val Asp Arg Ala Lys Leu 530 535 540 Asp Ala Leu Leu Ala Glu Leu Ala Glu Asp Pro Ala Ile Arg Gly Leu 545 550 555 560 Pro Ala Arg Ser Ala 565 <210> 3 <211> 1722 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Saccharothrix sp. ST-888 <400> 3 atgagtcgta ccgatactct gacggtgcat attggtcacg acgaattagc tcgcccggat 60 gttctgatcc caccattgga tggccctggg ctgtgcgtac cgcagggacc tgcggagccc 120 gacgtctgtg actttgaact ccctgcaggt ggcctggatg atgaacagcg tttacgcgca 180 ctggatacca tggacgagta tctgacaggt aaacgccgct accttctcgg ctatcaggca 240 acccaggaaa tgggtggaag cgcgttcgat ttaggccgtt ttatgcagca taacattaat 300 aatttgggtg atcccttcca atctggcggg tacaaaccaa acactaaggt ggtggaacgt 360 gccgttctgg actattatgc ggcgttatgg cacgctaaag gcccctacaa cgccgatgat 420 ccacagtcgt attggggcta cgtcctttca atgggtagca cggagggcaa tatgtatgca 480 ttgtggaacg cgcgtgacta cctctccggt aaagccttaa tccagccacc taccggtccc 540 agtggcgcgt tgcgctatgt tcaagcacct tctgaaccat ccaaccccaa tgcttaccgt 600 cctgtggcct tctatagcga agacacgcat tacagctttg cgaaagcagt acacgtgctt 660 ggagtcgaga cctttcatgc tgttggcaca gaacagtatc cagatgagtg ccctttagca 720 ggtccaaccg cggaacctcg cgaatggcca gcggaagtac cctcacgctc gggtccttcg 780 ggccatagtt gggatggcac gggagagatt gatatcgatg ctctcgcagt gttggtcgaa 840 ttctttgcgg cgaaaggtca ccccattttt gtgagcctca acttaggctc cactttcaag 900 ggcgctcatg acgacgttcg tcaagtgtgt gaacgccttc tccccatctt tgagaaacac 960 ggtttggttc agggtgaagt cgtctatggc aaagatccag tgaccggcga acctcttgtt 1020 gatttgcgtc gtcgcttctg gattcatgta gatggagcat taggtgcggg atacgcgcca 1080 tttatgcgca tggctgacgc agatccagcg ggctttggtt ggacacctga cacccgtgtg 1140 cccgaattcg attttggcat caccttacca actcgcgagt tcggtgacct cgatatggtg 1200 agcagcattg cgatgtctgg ccacaaatgg cctggtgcac catggccctg cggcatctat 1260 atgacgaagg tcaaatacca gatttcgcct ccttcccaac ccgattatat tggtgctcca 1320 gacaccacgt ttgcaggctc acgtaatgga ttctcacccc ttgttttgtg ggatcatctt 1380 agtcgccatt cttaccgtga tcaggtagat cgcatccgtc gtgcgcaaga attagcggcg 1440 tacctcgaac agcagttgcg ctttttagaa cgcgagaaag acattgaatt atggccagct 1500 cgtacgcccg gcgcaatcac cgtgcgcttt cgtaaaccta gtccagaact cgttgctaag 1560 tggagcttgt cgtcgcaaga tgtgcttttg gttcctggtg acgagaccac acgtcgcagc 1620 tatgtccacg tgttcctcat gtcctccgta gatcgtgcga aacttgacgc gctcttggaa 1680 gatcttggca atgatcccgc agttttacgc cgcccataat ag 1722 <210> 4 <211> 572 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Saccharothrix sp. ST-888 <400> 4 Met Ser Arg Thr Asp Thr Leu Thr Val His Ile Gly His Asp Glu Leu 1 5 10 15 Ala Arg Pro Asp Val Leu Ile Pro Pro Leu Asp Gly Pro Gly Leu Cys 20 25 30 Val Pro Gln Gly Pro Ala Glu Pro Asp Val Cys Asp Phe Glu Leu Pro 35 40 45 Ala Gly Gly Leu Asp Asp Glu Gln Arg Leu Arg Ala Leu Asp Thr Met 50 55 60 Asp Glu Tyr Leu Thr Gly Lys Arg Arg Tyr Leu Leu Gly Tyr Gln Ala 65 70 75 80 Thr Gln Glu Met Gly Gly Ser Ala Phe Asp Leu Gly Arg Phe Met Gln 85 90 95 His Asn Ile Asn Asn Leu Gly Asp Pro Phe Gln Ser Gly Gly Tyr Lys 100 105 110 Pro Asn Thr Lys Val Val Glu Arg Ala Val Leu Asp Tyr Tyr Ala Ala 115 120 125 Leu Trp His Ala Lys Gly Pro Tyr Asn Ala Asp Asp Pro Gln Ser Tyr 130 135 140 Trp Gly Tyr Val Leu Ser Met Gly Ser Thr Glu Gly Asn Met Tyr Ala 145 150 155 160 Leu Trp Asn Ala Arg Asp Tyr Leu Ser Gly Lys Ala Leu Ile Gln Pro 165 170 175 Pro Thr Gly Pro Ser Gly Ala Leu Arg Tyr Val Gln Ala Pro Ser Glu 180 185 190 Pro Ser Asn Pro Asn Ala Tyr Arg Pro Val Ala Phe Tyr Ser Glu Asp 195 200 205 Thr His Tyr Ser Phe Ala Lys Ala Val His Val Leu Gly Val Glu Thr 210 215 220 Phe His Ala Val Gly Thr Glu Gln Tyr Pro Asp Glu Cys Pro Leu Ala 225 230 235 240 Gly Pro Thr Ala Glu Pro Arg Glu Trp Pro Ala Glu Val Pro Ser Arg 245 250 255 Ser Gly Pro Ser Gly His Ser Trp Asp Gly Thr Gly Glu Ile Asp Ile 260 265 270 Asp Ala Leu Ala Val Leu Val Glu Phe Phe Ala Ala Lys Gly His Pro 275 280 285 Ile Phe Val Ser Leu Asn Leu Gly Ser Thr Phe Lys Gly Ala His Asp 290 295 300 Asp Val Arg Gln Val Cys Glu Arg Leu Leu Pro Ile Phe Glu Lys His 305 310 315 320 Gly Leu Val Gln Gly Glu Val Val Tyr Gly Lys Asp Pro Val Thr Gly 325 330 335 Glu Pro Leu Val Asp Leu Arg Arg Arg Phe Trp Ile His Val Asp Gly 340 345 350 Ala Leu Gly Ala Gly Tyr Ala Pro Phe Met Arg Met Ala Asp Ala Asp 355 360 365 Pro Ala Gly Phe Gly Trp Thr Pro Asp Thr Arg Val Pro Glu Phe Asp 370 375 380 Phe Gly Ile Thr Leu Pro Thr Arg Glu Phe Gly Asp Leu Asp Met Val 385 390 395 400 Ser Ser Ile Ala Met Ser Gly His Lys Trp Pro Gly Ala Pro Trp Pro 405 410 415 Cys Gly Ile Tyr Met Thr Lys Val Lys Tyr Gln Ile Ser Pro Pro Ser 420 425 430 Gln Pro Asp Tyr Ile Gly Ala Pro Asp Thr Thr Phe Ala Gly Ser Arg 435 440 445 Asn Gly Phe Ser Pro Leu Val Leu Trp Asp His Leu Ser Arg His Ser 450 455 460 Tyr Arg Asp Gln Val Asp Arg Ile Arg Arg Ala Gln Glu Leu Ala Ala 465 470 475 480 Tyr Leu Glu Gln Gln Leu Arg Phe Leu Glu Arg Glu Lys Asp Ile Glu 485 490 495 Leu Trp Pro Ala Arg Thr Pro Gly Ala Ile Thr Val Arg Phe Arg Lys 500 505 510 Pro Ser Pro Glu Leu Val Ala Lys Trp Ser Leu Ser Ser Gln Asp Val 515 520 525 Leu Leu Val Pro Gly Asp Glu Thr Thr Arg Arg Ser Tyr Val His Val 530 535 540 Phe Leu Met Ser Ser Val Asp Arg Ala Lys Leu Asp Ala Leu Leu Glu 545 550 555 560 Asp Leu Gly Asn Asp Pro Ala Val Leu Arg Arg Pro 565 570 <210> 5 <211> 1779 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Kitasatospora sp. MBT63 <400> 5 atgcgtcgta ccgatacgtt aacattggac ctgggtcatg gccacggtgc gccggcacgc 60 agcgatgccc gcatgtcacc acttggcgtg ccggctcagg caagtgcgcc ttctccggcc 120 ccgggcggac cactcgcgcc tgctgaagaa ccggcgctgt gcgactttct gctgccggca 180 gatgggctgg atgatgagca acgtttacgc gcattggaca ctatggatgc ctatctgacc 240 ggtaaacgcc gctacctgct gggctatcag gcaacccagg aaatgggtgg ttcggccttc 300 gaccttggcc gttttatgca tcataacatt aataatctcg gagatccctt ccaaagcggc 360 ggctacaaac cgaacacgaa ggttgttgag cgtgccgtcc tggactatta tgcggcgtta 420 tggcacgcgg atggtccata cgatgccgcc gatccgcagt cctattgggg ctacgtgttg 480 tcgatgggta gcaccgaagg gaacatgtat gcactgtgga atgctcgcga ctaccttagt 540 ggcaaagcat taatccagcc tcctactggt ccatgtggag cggcgcgtta cgtaaaagct 600 ccggcagaac cgggtaaccc gaatgcctat cgcccagtgg cgttctattc cgaagatacg 660 cactacagct ttgcgaaagc ggtccatgta ctcggcgtgg agacctttca tgccgttggc 720 accgaacaat atccggatga atgccccttg ccggctgctg caggaggtgc agcagccggc 780 gccgcctctg gtcctcgtga gtggcccgct gaagtgccct cacacagtgg cccaagcggt 840 cattcttggg atggaacggg cgagattgac gtcgaagccc ttgctgttct cgtggaattc 900 tttgcagcca agggccaccc gatctttgtc tcgttaaact tgggctccac attcaaaggt 960 gctcatgacg acgtacgcgg tgtgtgtgaa cgtcttctcc cgatttttga gaaatacggt 1020 ttaatccagg gcgaagttgc ctatggaaaa gatccgcgca ccggcgaacc gttggttgat 1080 ttacgtcgtc gcttctggat tcacgtggat ggcgcactcg gtgcaggcta tgccccgttt 1140 ttgcgcatgg ctgctgacga tccagcaggt tttggatggg agccggatac tcgtcttccc 1200 gaattcgact ttggcatcac cttacctacg cgcgaattcg gtgacctcga tatggtgtca 1260 agcattgcca tgagcggtca taagtggcct ggtgcaccat ggccatgcgg catctacatg 1320 accaaagtca aatatcagat tgcacctccg tctcaacccg attacattgg cgcccccgat 1380 acgacttttg caggatcgcg taacggcttc tcccctctta tcttgtggga ccatttaagt 1440 cgccacagct atcaggatca agtagatcgt attcgtcgtg cccaggactt ggctgcttac 1500 ctcgaacaac aacttcgttt gttggagctc gagaaagatg tcgaattatg gccagcacgc 1560 acaccaggtg ccatcaccgt tcgctttcgc aaacctagtc ccgaacttgt agccaagtgg 1620 agcctttcat cacaggatgt gttaaccgtt ccaggcgacg aatctacgcg ccgctcctat 1680 gtgcatgtgt tcctcatgtc gtcggttgat cgtgccaaat tagacgcatt gttggctgat 1740 ctcggtaatg atcccgtgat tttacgccgc ccataatag 1779 <210> 6 <211> 591 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Kitasatospora sp. MBT63 <400> 6 Met Arg Arg Thr Asp Thr Leu Thr Leu Asp Leu Gly His Gly His Gly 1 5 10 15 Ala Pro Ala Arg Ser Asp Ala Arg Met Ser Pro Leu Gly Val Pro Ala 20 25 30 Gln Ala Ser Ala Pro Ser Pro Ala Pro Gly Gly Pro Leu Ala Pro Ala 35 40 45 Glu Glu Pro Ala Leu Cys Asp Phe Leu Leu Pro Ala Asp Gly Leu Asp 50 55 60 Asp Glu Gln Arg Leu Arg Ala Leu Asp Thr Met Asp Ala Tyr Leu Thr 65 70 75 80 Gly Lys Arg Arg Tyr Leu Leu Gly Tyr Gln Ala Thr Gln Glu Met Gly 85 90 95 Gly Ser Ala Phe Asp Leu Gly Arg Phe Met His His Asn Ile Asn Asn 100 105 110 Leu Gly Asp Pro Phe Gln Ser Gly Gly Tyr Lys Pro Asn Thr Lys Val 115 120 125 Val Glu Arg Ala Val Leu Asp Tyr Tyr Ala Ala Leu Trp His Ala Asp 130 135 140 Gly Pro Tyr Asp Ala Ala Asp Pro Gln Ser Tyr Trp Gly Tyr Val Leu 145 150 155 160 Ser Met Gly Ser Thr Glu Gly Asn Met Tyr Ala Leu Trp Asn Ala Arg 165 170 175 Asp Tyr Leu Ser Gly Lys Ala Leu Ile Gln Pro Pro Thr Gly Pro Cys 180 185 190 Gly Ala Ala Arg Tyr Val Lys Ala Pro Ala Glu Pro Gly Asn Pro Asn 195 200 205 Ala Tyr Arg Pro Val Ala Phe Tyr Ser Glu Asp Thr His Tyr Ser Phe 210 215 220 Ala Lys Ala Val His Val Leu Gly Val Glu Thr Phe His Ala Val Gly 225 230 235 240 Thr Glu Gln Tyr Pro Asp Glu Cys Pro Leu Pro Ala Ala Ala Gly Gly 245 250 255 Ala Ala Ala Gly Ala Ala Ser Gly Pro Arg Glu Trp Pro Ala Glu Val 260 265 270 Pro Ser His Ser Gly Pro Ser Gly His Ser Trp Asp Gly Thr Gly Glu 275 280 285 Ile Asp Val Glu Ala Leu Ala Val Leu Val Glu Phe Phe Ala Ala Lys 290 295 300 Gly His Pro Ile Phe Val Ser Leu Asn Leu Gly Ser Thr Phe Lys Gly 305 310 315 320 Ala His Asp Asp Val Arg Gly Val Cys Glu Arg Leu Leu Pro Ile Phe 325 330 335 Glu Lys Tyr Gly Leu Ile Gln Gly Glu Val Ala Tyr Gly Lys Asp Pro 340 345 350 Arg Thr Gly Glu Pro Leu Val Asp Leu Arg Arg Arg Phe Trp Ile His 355 360 365 Val Asp Gly Ala Leu Gly Ala Gly Tyr Ala Pro Phe Leu Arg Met Ala 370 375 380 Ala Asp Asp Pro Ala Gly Phe Gly Trp Glu Pro Asp Thr Arg Leu Pro 385 390 395 400 Glu Phe Asp Phe Gly Ile Thr Leu Pro Thr Arg Glu Phe Gly Asp Leu 405 410 415 Asp Met Val Ser Ser Ile Ala Met Ser Gly His Lys Trp Pro Gly Ala 420 425 430 Pro Trp Pro Cys Gly Ile Tyr Met Thr Lys Val Lys Tyr Gln Ile Ala 435 440 445 Pro Pro Ser Gln Pro Asp Tyr Ile Gly Ala Pro Asp Thr Thr Phe Ala 450 455 460 Gly Ser Arg Asn Gly Phe Ser Pro Leu Ile Leu Trp Asp His Leu Ser 465 470 475 480 Arg His Ser Tyr Gln Asp Gln Val Asp Arg Ile Arg Arg Ala Gln Asp 485 490 495 Leu Ala Ala Tyr Leu Glu Gln Gln Leu Arg Leu Leu Glu Leu Glu Lys 500 505 510 Asp Val Glu Leu Trp Pro Ala Arg Thr Pro Gly Ala Ile Thr Val Arg 515 520 525 Phe Arg Lys Pro Ser Pro Glu Leu Val Ala Lys Trp Ser Leu Ser Ser 530 535 540 Gln Asp Val Leu Thr Val Pro Gly Asp Glu Ser Thr Arg Arg Ser Tyr 545 550 555 560 Val His Val Phe Leu Met Ser Ser Val Asp Arg Ala Lys Leu Asp Ala 565 570 575 Leu Leu Ala Asp Leu Gly Asn Asp Pro Val Ile Leu Arg Arg Pro 580 585 590 <210> 7 <211> 1665 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Kitasatospora sp. MMS16-BH015 <400> 7 atgagtccgt ttgaaaccat gaatcctgcg gcggatcagg agctgccacc attagccttg 60 gctggccatc gtcttccgca accgccggca gaagaacctc aggtggccga gtatgcaatt 120 ccagttggtg gtttagaccg cgaacaacgt ctgcgcgctc tcgatcgcat cgatacgtac 180 cttgcacaca aacgtcagca tttgttgggc tatcaggcta accaggaact cgatggatgc 240 gcgtctgact taggtcgttt catgcgccac aacattaata atttaggcga tccctttcgt 300 agcggcgggt acaaacccaa cactaaggcc gtcgaacatg cagtactcga ctattatgcc 360 gatctttggc gcgcgaaagg tcctcactcg ccagaggatc ccgaatccta ctgggggtat 420 gcgctttcaa tgggaagcac cgaaggcaac atgtacgcgt tgtggaatgc ccgcgactat 480 ctcagcggta agcctttaat ccgtcctaca gcatctagtg gctcaggagc agtggtgacg 540 ccaaactcgc gtcgcccagt tgccttctac agcgaggaca cccattattc ctttgcgaaa 600 gctgtacgtg ttttgggtgt cgaaaccttc cacgatattg gcgtggaaaa ctatccaggt 660 cagtgtccac tccctggcag tggtggtgtg tggccccttg aagttcccag tgttcctggt 720 ccctcgggat tatcttggga tggcacgggc gaaatcagcg tggatgcgct cgaattgttg 780 gtcgagttct ttgcggctaa aggccatccc gtctttgtgt cccttaattt aggcagcacc 840 tttaaaggtg cccatgacga cgtacgcgca gtttgcgaac gtttgctccc cattttcgaa 900 cgctacggca tggtggagtc agaggtcgta cgcggtcgtg atcaacgtag cggcgagctt 960 cttactgatc gccgccgtcg cttctggatc cacgtggacg gagccttagg tgcggcctac 1020 ctcccttttt tacgtttggc gcaggaatcc ccagaaacat atggttggac accagctgca 1080 ccagcgcctg agtttgattt tggcttgcgc ttaccaaccg ctggccatgg cgaagttgat 1140 ctcgtcagct cgattgccat gtctggacac aaatggcctg gtgttccttg gccctgtggc 1200 atctacatga cgaaagtgaa gtatcaagtg agtcctccct ctcagccaga ctataccggt 1260 gcacctgata ctactttcgc gggctcacgt aacggctttt cgccacttgt actctgggat 1320 catttagccc gccatagcca cgccgaccaa gtcgatcgta ttcgccgctc ccaggaattg 1380 gcggcatacc ttgaaagccg tttaaccgca ttggaacgta cccatggtct taccttatgg 1440 cctgctcgca caccaggagc tttaaccgtt cgtttccgta aacctagtcc cgaactcgtg 1500 actaaatggt cgttgtcagc ccaggatgtt ctcacgactc caggtgaaga agcaacccgc 1560 cgctcctatg tgcacgtctt cttaatgcca agcgtaaccc gtgagaaatt ggatgcgctc 1620 cttgatgacc tcgtgaatga ccccgtgatt acaggctcct aatag 1665 <210> 8 <211> 553 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Kitasatospora sp. MMS16-BH015 <400> 8 Met Ser Pro Phe Glu Thr Met Asn Pro Ala Ala Asp Gln Glu Leu Pro 1 5 10 15 Pro Leu Ala Leu Ala Gly His Arg Leu Pro Gln Pro Pro Ala Glu Glu 20 25 30 Pro Gln Val Ala Glu Tyr Ala Ile Pro Val Gly Gly Leu Asp Arg Glu 35 40 45 Gln Arg Leu Arg Ala Leu Asp Arg Ile Asp Thr Tyr Leu Ala His Lys 50 55 60 Arg Gln His Leu Leu Gly Tyr Gln Ala Asn Gln Glu Leu Asp Gly Cys 65 70 75 80 Ala Ser Asp Leu Gly Arg Phe Met Arg His Asn Ile Asn Asn Leu Gly 85 90 95 Asp Pro Phe Arg Ser Gly Gly Tyr Lys Pro Asn Thr Lys Ala Val Glu 100 105 110 His Ala Val Leu Asp Tyr Tyr Ala Asp Leu Trp Arg Ala Lys Gly Pro 115 120 125 His Ser Pro Glu Asp Pro Glu Ser Tyr Trp Gly Tyr Ala Leu Ser Met 130 135 140 Gly Ser Thr Glu Gly Asn Met Tyr Ala Leu Trp Asn Ala Arg Asp Tyr 145 150 155 160 Leu Ser Gly Lys Pro Leu Ile Arg Pro Thr Ala Ser Ser Gly Ser Gly 165 170 175 Ala Val Val Thr Pro Asn Ser Arg Arg Pro Val Ala Phe Tyr Ser Glu 180 185 190 Asp Thr His Tyr Ser Phe Ala Lys Ala Val Arg Val Leu Gly Val Glu 195 200 205 Thr Phe His Asp Ile Gly Val Glu Asn Tyr Pro Gly Gln Cys Pro Leu 210 215 220 Pro Gly Ser Gly Gly Val Trp Pro Leu Glu Val Pro Ser Val Pro Gly 225 230 235 240 Pro Ser Gly Leu Ser Trp Asp Gly Thr Gly Glu Ile Ser Val Asp Ala 245 250 255 Leu Glu Leu Leu Val Glu Phe Phe Ala Ala Lys Gly His Pro Val Phe 260 265 270 Val Ser Leu Asn Leu Gly Ser Thr Phe Lys Gly Ala His Asp Asp Val 275 280 285 Arg Ala Val Cys Glu Arg Leu Leu Pro Ile Phe Glu Arg Tyr Gly Met 290 295 300 Val Glu Ser Glu Val Val Arg Gly Arg Asp Gln Arg Ser Gly Glu Leu 305 310 315 320 Leu Thr Asp Arg Arg Arg Arg Phe Trp Ile His Val Asp Gly Ala Leu 325 330 335 Gly Ala Ala Tyr Leu Pro Phe Leu Arg Leu Ala Gln Glu Ser Pro Glu 340 345 350 Thr Tyr Gly Trp Thr Pro Ala Ala Pro Ala Pro Glu Phe Asp Phe Gly 355 360 365 Leu Arg Leu Pro Thr Ala Gly His Gly Glu Val Asp Leu Val Ser Ser 370 375 380 Ile Ala Met Ser Gly His Lys Trp Pro Gly Val Pro Trp Pro Cys Gly 385 390 395 400 Ile Tyr Met Thr Lys Val Lys Tyr Gln Val Ser Pro Pro Ser Gln Pro 405 410 415 Asp Tyr Thr Gly Ala Pro Asp Thr Thr Phe Ala Gly Ser Arg Asn Gly 420 425 430 Phe Ser Pro Leu Val Leu Trp Asp His Leu Ala Arg His Ser His Ala 435 440 445 Asp Gln Val Asp Arg Ile Arg Arg Ser Gln Glu Leu Ala Ala Tyr Leu 450 455 460 Glu Ser Arg Leu Thr Ala Leu Glu Arg Thr His Gly Leu Thr Leu Trp 465 470 475 480 Pro Ala Arg Thr Pro Gly Ala Leu Thr Val Arg Phe Arg Lys Pro Ser 485 490 495 Pro Glu Leu Val Thr Lys Trp Ser Leu Ser Ala Gln Asp Val Leu Thr 500 505 510 Thr Pro Gly Glu Glu Ala Thr Arg Arg Ser Tyr Val His Val Phe Leu 515 520 525 Met Pro Ser Val Thr Arg Glu Lys Leu Asp Ala Leu Leu Asp Asp Leu 530 535 540 Val Asn Asp Pro Val Ile Thr Gly Ser 545 550 <210> 9 <211> 1620 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Streptomyces sp. NRRL F-6131 <400> 9 atgagtccaa ccaccgcgcg tgctgcagtt gcacgcgcgg cgctggatgg cgccttagac 60 cgtgctgtcg tggatcgcgc cgtagtagat cgtgccgtgg ttgaaggtgc agctgttcct 120 ggaccggtcc atgttgacgc agagccggaa ggcgccgatt ttgcaattcc ggccgaaggt 180 ctgacggatg gcgaacgttt acgcgccctt gaccgcatgg atgggtatct gactcgtaaa 240 cgtcgtcacc tcgtgggtta ccaggctaca caagagctgg ctggtagcgc cctggattta 300 gcacgcttca tgctgtccaa catcaataat ttgggcgacc cctttcagtc tggcgggtat 360 aaaccgaaca cgaaggtcgt cgaacgcgcc gtactggatt actacgcccg cctttggcat 420 gcaggtccat cgtatgaccc tgcagatccg gagtcatatt ggggctatat gctgagcatg 480 ggaagtaccg aaggcaacat gtacgccctc tggaatgctc gcgactattt aactcgtcca 540 ggtgcaccgc gtccggttgc cttctacagc gaagataccc actatagctt tgctaaagcc 600 gttcgcgtac tgggtgtcga tacgttccat gcagtaggaa ccgccgatta ccccgatgaa 660 tgccctcttg gtggcgagtg gccggctgaa gttccgtctc agccaggccc ttcaggtttg 720 tcgtgggacg gcagtggcgc agtcgactta gatgcactgg ccgccctggt cgagttcttt 780 gctgctaaag gtcacccggt ctttgttaac tttaatttgg gctccacctt caaaggagcc 840 catgatgatg tacgtggtgc atgtgaacgc ctcctcccga tttttgaacg ccatggctta 900 ttaccacgtg caggttcaga tgctcgtcgt ggcttctgga tccacgttga tggtgcattg 960 ggtgccggtt atgccccctt tcttcgcatg gcttctgcag atcctgccgc atttggctgg 1020 gaaccggagg ccgaactccc ggaattcgac tttggcctta cgttggctac acgcgaaagc 1080 ggcgacgtag atccagttgc atcgattgcc atgagcggac ataaatgggc tggtgtaccc 1140 tggccatgcg gcgtcttcat gaccaagact aaatatcaac ttgaacctcc tacgcagccc 1200 gagtacattg gtgccccaga ctccaccttt gccggaagtc gtaacggctt tagcccattg 1260 gttttatggg atcacttatc tcgcctctcg catcgtgatc aagtcgatcg tatccgccgc 1320 tcccaggaac tcgctgcata tcttgagcgt cgtttgcgcg aagttgaacg cgaaacaggc 1380 gcagaattgt ggccagcacg cacgcctggt gctatcaccg tacgtttccg taaaccttca 1440 gccggattgg ttgccaagtg gagccttagt tcggagaacg ttttaacaac tcccggtgat 1500 cctgccggct tacgtggcta cgtacacgtc tttctcatgc catcagtcga ccgtgctaaa 1560 ttagacgctc ttctcgacga tttggctcgt gatcccgtca ttcttggtcg cgcttaatag 1620 <210> 10 <211> 538 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Streptomyces sp. NRRL F-6131 <400> 10 Met Ser Pro Thr Thr Ala Arg Ala Ala Val Ala Arg Ala Ala Leu Asp 1 5 10 15 Gly Ala Leu Asp Arg Ala Val Val Asp Arg Ala Val Val Asp Arg Ala 20 25 30 Val Val Glu Gly Ala Ala Val Pro Gly Pro Val His Val Asp Ala Glu 35 40 45 Pro Glu Gly Ala Asp Phe Ala Ile Pro Ala Glu Gly Leu Thr Asp Gly 50 55 60 Glu Arg Leu Arg Ala Leu Asp Arg Met Asp Gly Tyr Leu Thr Arg Lys 65 70 75 80 Arg Arg His Leu Val Gly Tyr Gln Ala Thr Gln Glu Leu Ala Gly Ser 85 90 95 Ala Leu Asp Leu Ala Arg Phe Met Leu Ser Asn Ile Asn Asn Leu Gly 100 105 110 Asp Pro Phe Gln Ser Gly Gly Tyr Lys Pro Asn Thr Lys Val Val Glu 115 120 125 Arg Ala Val Leu Asp Tyr Tyr Ala Arg Leu Trp His Ala Gly Pro Ser 130 135 140 Tyr Asp Pro Ala Asp Pro Glu Ser Tyr Trp Gly Tyr Met Leu Ser Met 145 150 155 160 Gly Ser Thr Glu Gly Asn Met Tyr Ala Leu Trp Asn Ala Arg Asp Tyr 165 170 175 Leu Thr Arg Pro Gly Ala Pro Arg Pro Val Ala Phe Tyr Ser Glu Asp 180 185 190 Thr His Tyr Ser Phe Ala Lys Ala Val Arg Val Leu Gly Val Asp Thr 195 200 205 Phe His Ala Val Gly Thr Ala Asp Tyr Pro Asp Glu Cys Pro Leu Gly 210 215 220 Gly Glu Trp Pro Ala Glu Val Pro Ser Gln Pro Gly Pro Ser Gly Leu 225 230 235 240 Ser Trp Asp Gly Ser Gly Ala Val Asp Leu Asp Ala Leu Ala Ala Leu 245 250 255 Val Glu Phe Phe Ala Ala Lys Gly His Pro Val Phe Val Asn Phe Asn 260 265 270 Leu Gly Ser Thr Phe Lys Gly Ala His Asp Asp Val Arg Gly Ala Cys 275 280 285 Glu Arg Leu Leu Pro Ile Phe Glu Arg His Gly Leu Leu Pro Arg Ala 290 295 300 Gly Ser Asp Ala Arg Arg Gly Phe Trp Ile His Val Asp Gly Ala Leu 305 310 315 320 Gly Ala Gly Tyr Ala Pro Phe Leu Arg Met Ala Ser Ala Asp Pro Ala 325 330 335 Ala Phe Gly Trp Glu Pro Glu Ala Glu Leu Pro Glu Phe Asp Phe Gly 340 345 350 Leu Thr Leu Ala Thr Arg Glu Ser Gly Asp Val Asp Pro Val Ala Ser 355 360 365 Ile Ala Met Ser Gly His Lys Trp Ala Gly Val Pro Trp Pro Cys Gly 370 375 380 Val Phe Met Thr Lys Thr Lys Tyr Gln Leu Glu Pro Pro Thr Gln Pro 385 390 395 400 Glu Tyr Ile Gly Ala Pro Asp Ser Thr Phe Ala Gly Ser Arg Asn Gly 405 410 415 Phe Ser Pro Leu Val Leu Trp Asp His Leu Ser Arg Leu Ser His Arg 420 425 430 Asp Gln Val Asp Arg Ile Arg Arg Ser Gln Glu Leu Ala Ala Tyr Leu 435 440 445 Glu Arg Arg Leu Arg Glu Val Glu Arg Glu Thr Gly Ala Glu Leu Trp 450 455 460 Pro Ala Arg Thr Pro Gly Ala Ile Thr Val Arg Phe Arg Lys Pro Ser 465 470 475 480 Ala Gly Leu Val Ala Lys Trp Ser Leu Ser Ser Glu Asn Val Leu Thr 485 490 495 Thr Pro Gly Asp Pro Ala Gly Leu Arg Gly Tyr Val His Val Phe Leu 500 505 510 Met Pro Ser Val Asp Arg Ala Lys Leu Asp Ala Leu Leu Asp Asp Leu 515 520 525 Ala Arg Asp Pro Val Ile Leu Gly Arg Ala 530 535 <210> 11 <211> 1224 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Leucine Decarboxylase from Planctomycetaceae bacterium <400> 11 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta atag 1224 <210> 12 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Leucine Decarboxylase from Planctomycetaceae bacterium <400> 12 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 13 <211> 1185 <212> DNA <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Larkinella arboricola <400> 13 atgaccgtga aagaagttga acgtctgcag cgctataaag atctgttagc ggaacgtagc 60 agcaacgcca ttggttaccc gtttgcactc gacttcgatt tttcggagtt gcatacgctg 120 ttccaatatc acatcaataa tgtgggcgac ccatacatcg agtccaacta cgatgtcaac 180 tctcatagta tggaacgcga ggtcgtagaa ttctttgctg atctgtttcg tgcgccgaag 240 cacaatcgct gggggtatgt tacttcagga ggatcggaag gtaaccttta tggtctgtat 300 gtggcccgcg aactgtatcc tgaagcggtg atttactact ccgcagctac ccattacagc 360 attagcaaat ctatccgttt gttgaatatg ccgtcgtgca tgattcgtat tcagcccaac 420 ggtgagatgg actataaaga tctggaagaa agtatcaaac gctattcaaa tcgtccggcc 480 atcgttgttg cgaacatcgg gacgacaatg accgaagcaa aggatgatgt accaaccatt 540 cgctccgtgc ttcagaaagc ggcggtgaaa agccactaca tccattgtga cgctgcctta 600 agtgggtctt atctggcagt cctcgaggag gaaccgcaat ttgacttcgc caacggtgtc 660 gatagcattg catgctcagg acacaagttc attggttcgc cttttccgtg tggtgtagtg 720 gtgatcaaag atcagtacaa agaacgcgtt tctaaacccg tcagctatat tgggtccttt 780 gacagtacta ttgctggaag ccgtaatggt cattcgccgg ttttcatgtg gtatgccatc 840 aaacagcagg ggcgcgaagg tctgctgcgt cgtgcgttag agtgcctgtc tctggcggaa 900 tacacacaac gccgcctcca ggaacatggt attgcggcgt ggcgtaacga aaaggcaatc 960 acggtggtgt ttccaaaacc gtccgcctca atctgtcgca aatggcaact tgccaccaat 1020 gcaggacagg ctcacctgat ttgcatgcca gggattagcc aacagcgcat cgatgatttt 1080 attgaggacc tgctgaaaga aaccagtttg attgcgcgtg aaccgacgca tagccgtgtc 1140 ttcgttaaac ctggtgattc gatcatgatt acccagctgt aatag 1185 <210> 14 <211> 393 <212> PRT <213> Artificial Sequence <220> <223> Wild Type Amino Acid Decarboxylase from Larkinella arboricola <400> 14 Met Thr Val Lys Glu Val Glu Arg Leu Gln Arg Tyr Lys Asp Leu Leu 1 5 10 15 Ala Glu Arg Ser Ser Asn Ala Ile Gly Tyr Pro Phe Ala Leu Asp Phe 20 25 30 Asp Phe Ser Glu Leu His Thr Leu Phe Gln Tyr His Ile Asn Asn Val 35 40 45 Gly Asp Pro Tyr Ile Glu Ser Asn Tyr Asp Val Asn Ser His Ser Met 50 55 60 Glu Arg Glu Val Val Glu Phe Phe Ala Asp Leu Phe Arg Ala Pro Lys 65 70 75 80 His Asn Arg Trp Gly Tyr Val Thr Ser Gly Gly Ser Glu Gly Asn Leu 85 90 95 Tyr Gly Leu Tyr Val Ala Arg Glu Leu Tyr Pro Glu Ala Val Ile Tyr 100 105 110 Tyr Ser Ala Ala Thr His Tyr Ser Ile Ser Lys Ser Ile Arg Leu Leu 115 120 125 Asn Met Pro Ser Cys Met Ile Arg Ile Gln Pro Asn Gly Glu Met Asp 130 135 140 Tyr Lys Asp Leu Glu Glu Ser Ile Lys Arg Tyr Ser Asn Arg Pro Ala 145 150 155 160 Ile Val Val Ala Asn Ile Gly Thr Thr Met Thr Glu Ala Lys Asp Asp 165 170 175 Val Pro Thr Ile Arg Ser Val Leu Gln Lys Ala Ala Val Lys Ser His 180 185 190 Tyr Ile His Cys Asp Ala Ala Leu Ser Gly Ser Tyr Leu Ala Val Leu 195 200 205 Glu Glu Glu Pro Gln Phe Asp Phe Ala Asn Gly Val Asp Ser Ile Ala 210 215 220 Cys Ser Gly His Lys Phe Ile Gly Ser Pro Phe Pro Cys Gly Val Val 225 230 235 240 Val Ile Lys Asp Gln Tyr Lys Glu Arg Val Ser Lys Pro Val Ser Tyr 245 250 255 Ile Gly Ser Phe Asp Ser Thr Ile Ala Gly Ser Arg Asn Gly His Ser 260 265 270 Pro Val Phe Met Trp Tyr Ala Ile Lys Gln Gln Gly Arg Glu Gly Leu 275 280 285 Leu Arg Arg Ala Leu Glu Cys Leu Ser Leu Ala Glu Tyr Thr Gln Arg 290 295 300 Arg Leu Gln Glu His Gly Ile Ala Ala Trp Arg Asn Glu Lys Ala Ile 305 310 315 320 Thr Val Val Phe Pro Lys Pro Ser Ala Ser Ile Cys Arg Lys Trp Gln 325 330 335 Leu Ala Thr Asn Ala Gly Gln Ala His Leu Ile Cys Met Pro Gly Ile 340 345 350 Ser Gln Gln Arg Ile Asp Asp Phe Ile Glu Asp Leu Leu Lys Glu Thr 355 360 365 Ser Leu Ile Ala Arg Glu Pro Thr His Ser Arg Val Phe Val Lys Pro 370 375 380 Gly Asp Ser Ile Met Ile Thr Gln Leu 385 390 <210> 15 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 15 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatgt cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 16 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 16 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 17 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 17 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 18 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 18 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 19 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 19 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 20 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 20 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 21 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 21 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 22 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 22 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 23 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 23 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 24 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 24 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 25 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 25 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 26 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 26 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 27 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 27 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 28 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 28 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 29 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 29 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 30 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 30 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 31 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 31 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatat cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 32 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 32 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 33 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 33 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 34 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 34 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 35 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 35 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 36 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 36 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 37 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 37 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 38 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 38 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 39 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 39 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 40 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 40 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 41 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 41 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 42 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 42 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 43 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 43 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 44 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 44 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 45 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 45 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaacct gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 46 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 46 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 47 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 47 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 48 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 48 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 49 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 49 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 50 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 50 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 51 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 51 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 52 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 52 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 53 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 53 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 54 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 54 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 55 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 55 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 56 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 56 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 57 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 57 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 58 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 58 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 59 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 59 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 60 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 60 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 61 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 61 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 62 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 62 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 63 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 63 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 64 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 64 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 65 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 65 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 66 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 66 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 67 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 67 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 68 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 68 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 69 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 69 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 70 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 70 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 71 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 71 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatgt cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 72 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 72 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 73 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 73 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttcc tggggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 74 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 74 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Leu Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 75 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 75 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 76 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 76 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 77 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 77 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttacgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 78 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 78 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 79 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 79 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 80 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 80 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 81 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 81 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaacggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 82 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 82 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 83 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 83 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtagag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 84 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 84 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 85 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 85 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc ggtaaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagtg ttgaatatgt cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 86 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 86 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Val Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Val Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 87 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 87 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagtaaat ggcgaccgcg cgcatattgt cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 88 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 88 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 89 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 89 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aagatccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 90 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 90 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 91 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 91 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccggc gccggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 92 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 92 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 93 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 93 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgttctatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 94 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 94 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Ser Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 95 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 95 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac ataggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 96 <211> 397 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 96 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr 385 390 395 <210> 97 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 97 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcaggggccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 98 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 98 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 99 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 99 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagcagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 100 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 100 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gln Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 101 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 101 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 102 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 102 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 103 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 103 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtcttacgta g 1221 <210> 104 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 104 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Leu Thr 405 <210> 105 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 105 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc taggccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 106 <211> 390 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 106 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro 385 390 <210> 107 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 107 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaga agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 108 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 108 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 109 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 109 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccacga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 110 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 110 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Thr Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 111 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 111 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcgctcgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 112 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 112 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Ala Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 113 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 113 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ttgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 114 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 114 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Ile Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 115 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 115 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccacct tgcagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 116 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 116 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 117 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 117 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gaggccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 118 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 118 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Glu Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 119 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 119 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaggct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 120 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 120 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Arg Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 121 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 121 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aagggtatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 122 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 122 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys Gly Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 123 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 123 atgggtaatc tgatgtttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 124 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 124 Met Gly Asn Leu Met Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 125 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 125 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaaggatct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 126 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 126 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Asp Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 127 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 127 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aaaagccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 128 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 128 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 129 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 129 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggcta agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 130 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 130 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Ala Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 131 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 131 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 132 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 132 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 133 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 133 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgacgagg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 134 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 134 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Glu Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 135 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 135 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccacca ggcagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 136 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 136 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 137 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 137 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcaggagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 138 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 138 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Glu Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 139 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 139 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttagt ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 140 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 140 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Ser Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 141 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 141 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gtcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 142 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 142 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 143 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 143 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aagctccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 144 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 144 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Ala Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 145 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 145 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 146 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 146 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 147 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 147 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg cgcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 148 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 148 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 149 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 149 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagcctccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 150 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 150 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Pro Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 151 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 151 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 152 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 152 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 153 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 153 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aaaattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 154 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 154 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys Asn Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 155 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 155 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tcgagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 156 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 156 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Glu His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 157 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 157 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggattca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 158 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 158 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Asp Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 159 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 159 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtcatacgta g 1221 <210> 160 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 160 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg His Thr 405 <210> 161 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 161 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gatgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 162 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 162 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Asp Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 163 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 163 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatatttg tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 164 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 164 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Cys Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 165 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 165 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtgagacgta g 1221 <210> 166 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 166 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Glu Thr 405 <210> 167 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 167 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtgatacgta g 1221 <210> 168 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 168 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Asp Thr 405 <210> 169 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 169 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgactggg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 170 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 170 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Trp Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 171 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 171 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacttgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga ctcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 172 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 172 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Thr Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 173 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 173 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gaggcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 174 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 174 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Glu Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 175 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 175 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctggggatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 176 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 176 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Gly Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 177 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 177 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcgttcgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 178 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 178 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Val Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 179 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 179 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caatccggcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 180 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 180 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Pro Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 181 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 181 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 182 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 182 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 183 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 183 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 184 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 184 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 185 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 185 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgacgatg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 186 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 186 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Asp Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 187 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 187 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 188 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 188 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 189 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 189 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aaggtccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 190 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 190 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Gly Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 191 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 191 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgacagtg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 192 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 192 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Ser Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 193 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 193 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aaagtccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 194 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 194 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Ser Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 195 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 195 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat gttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 196 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 196 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Met Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 197 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 197 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagctagac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 198 <211> 385 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 198 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser 385 <210> 199 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 199 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgacaatg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 200 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 200 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Asn Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 201 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 201 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccttg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 202 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 202 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Leu Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 203 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 203 atgggtaatc tgaaatttcc agatggcgcg ttgagcccgc atgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca ttgggtatcc gtgtacgatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtaggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggaaccgagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagacac ccattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgattccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatgt cggtaccctg gacactacga ttaccggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcagttaac ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcagccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 204 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 204 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro His Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Thr Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Thr Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Thr His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Ile Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Val Gly Thr Leu Asp Thr 260 265 270 Thr Ile Thr Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Val Asn Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 205 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 205 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatatttg cttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 206 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 206 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Cys Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 207 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 207 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgactggg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 208 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 208 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Trp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 209 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 209 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcga gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 210 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 210 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 211 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 211 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat gttaatgcca 1080 agtaccaccc tgcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 212 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 212 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Met Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 213 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 213 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga atcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgactggg cgcatatttg cttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 214 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 214 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Asn Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Trp Ala His Ile Cys Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 215 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 215 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttcccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat gttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 216 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 216 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Met Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 217 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 217 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgacaatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 218 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 218 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asn Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 219 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 219 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgactggg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 220 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 220 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Trp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 221 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 221 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgacgatg cgcatattgt gttaatgcca 1080 agtaccaccc tgcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 222 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 222 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Val Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 223 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 223 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 224 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 224 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 225 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 225 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccgcg cgcatatttg cttaatgcca 1080 agtaccaccc tgcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 226 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 226 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Cys Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 227 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 227 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat tttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 228 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 228 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 229 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 229 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttcccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 230 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 230 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 231 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 231 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcga gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 232 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 232 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 233 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 233 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 234 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 234 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 235 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 235 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttcccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgagg cgcatatttg cttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 236 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 236 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Glu Ala His Ile Cys Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 237 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 237 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 238 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 238 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Met Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 239 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 239 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgagg cgcatatttg cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 240 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 240 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Glu Ala His Ile Cys Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 241 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 241 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgacgatg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 242 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 242 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 243 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 243 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatattgt gttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 244 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 244 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Val Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 245 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 245 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttcccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgacaatg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 246 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 246 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asn Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 247 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 247 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca tccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt gttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 248 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 248 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 249 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 249 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgct gccatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgacgatg cgcatattgt gttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 250 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 250 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Cys His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Val Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 251 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 251 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgaccgcg cgcatattgt gttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 252 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 252 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Val Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 253 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 253 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaaggatct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgaccgcg cgcatatttg cttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 254 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 254 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Asp Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Cys Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 255 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 255 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaaggatct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaagatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcata agtggcaaat cgcaaccgag ggcgacaatg cgcatatttg cttaatgcca 1080 agtaccacca agcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 256 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 256 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Asp Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asn Ala His Ile Cys Leu Met Pro Ser Thr Thr Lys Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 257 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 257 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgtatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccgcg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 258 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 258 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 259 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 259 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcaggggccc gccgccaagg aagatccggc cccggctggt 1200 gcaccgcaac gtgacacgta g 1221 <210> 260 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 260 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Asp Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Asp Thr 405 <210> 261 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 261 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtttaaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aagatccgac cccggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 262 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 262 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Phe Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 263 <211> 1158 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 263 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagctaa 1158 <210> 264 <211> 385 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 264 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser 385 <210> 265 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 265 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc taagccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 266 <211> 390 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 266 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro 385 390 <210> 267 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 267 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcaggggccc gccgccaaga aaaaaccgac cccggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 268 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 268 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 269 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 269 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg aagatccggc cccggctggt 1200 gcaccgcaaa ttcacacgta g 1221 <210> 270 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 270 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Asp Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Ile His Thr 405 <210> 271 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 271 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg aaaaaccggc cccggctggt 1200 gcaccgcaac gtgacacgta g 1221 <210> 272 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 272 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Asp Thr 405 <210> 273 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 273 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta tcgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg aaaaaccgac cccggctggt 1200 gcaccgcaac gtgacacgta g 1221 <210> 274 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 274 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Asp Thr 405 <210> 275 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 275 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg aacgtccggc cccggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 276 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 276 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 277 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 277 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgca ttcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgc gtcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctgcaca agtggcaaat cgcaaccgag ggcgaccgcg cgcatattat cttaatgcca 1080 agtaccaccc tccagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcaggggccc gccgccaagg aagatccgac cccggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 278 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 278 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ile His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Arg Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu His Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Arg Ala His Ile Ile Leu Met Pro Ser Thr Thr Leu Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 279 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 279 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tctgccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaaaatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcttca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 280 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 280 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 281 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 281 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 282 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 282 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 283 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 283 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 284 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 284 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 285 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 285 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 286 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 286 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 287 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 287 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtcacacg 1218 <210> 288 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 288 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg His Thr 405 <210> 289 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 289 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tctgccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa aaaaatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 290 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 290 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Lys Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 291 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 291 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 292 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 292 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 293 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 293 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaatca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 294 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 294 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 295 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 295 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcttca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 296 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 296 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 297 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 297 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg catccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcttca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 298 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 298 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 299 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 299 atggagaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 300 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 300 Met Glu Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 301 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 301 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagag 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 302 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 302 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 303 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 303 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaggtg 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 304 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 304 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Val Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 305 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 305 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctagtgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 306 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 306 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ser Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 307 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 307 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaagcg gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 308 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 308 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Ala Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 309 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 309 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 gtgatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 310 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 310 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Val Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 311 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 311 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gttccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 312 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 312 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 313 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 313 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatattt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 314 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 314 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Ile Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 315 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 315 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcattcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 316 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 316 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 317 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 317 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagacg 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 318 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 318 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Thr Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 319 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 319 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcgtta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 320 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 320 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Val Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 321 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 321 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaagag gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 322 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 322 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Glu Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 323 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 323 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 324 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 324 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 325 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 325 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaatag gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 326 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 326 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 327 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 327 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaagt gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 328 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 328 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Ser Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 329 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 329 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 tagccgcaac gtactacg 1218 <210> 330 <211> 400 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 330 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 <210> 331 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 331 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 332 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 332 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 333 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 333 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 tatccgcaac gtactacg 1218 <210> 334 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 334 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Tyr Pro Gln Arg Thr Thr 405 <210> 335 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 335 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccctga tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 336 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 336 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Leu Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 337 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 337 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagttgatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 338 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 338 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Leu Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 339 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 339 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aggacccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 340 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 340 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Asp Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 341 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 341 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaagg agcgcccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 342 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 342 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 343 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 343 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga agcgcccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 344 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 344 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 345 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 345 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga agaagccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 346 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 346 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 347 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 347 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga accgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg agaagccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 348 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 348 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Asn Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 349 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 349 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaaga agcgcccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 350 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 350 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 351 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 351 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg aggacccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 352 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 352 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 353 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 353 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg aggacccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 354 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 354 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Asp Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 355 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 355 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg agaagccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 356 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 356 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 357 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 357 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg aggacccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 358 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 358 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 359 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 359 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg aggacccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 360 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 360 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 361 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 361 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg agcgcccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 362 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 362 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 363 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 363 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga accgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aggacccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 364 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 364 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Asn Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 365 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 365 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agcgcccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 366 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 366 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 367 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 367 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaaga aggacccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 368 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 368 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Asp Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 369 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 369 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga agaagccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 370 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 370 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 371 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 371 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg agaagccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 372 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 372 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 373 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 373 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agaagccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 374 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 374 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Lys Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 375 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 375 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agcgcccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 376 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 376 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 377 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 377 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaaga agaagccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 378 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 378 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 379 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 379 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaaga agcgcccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 380 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 380 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 381 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 381 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agaagccgac tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 382 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 382 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Lys Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 383 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 383 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga accgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 384 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 384 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Asn Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 385 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 385 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agcgcccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 386 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 386 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 387 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 387 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggcccc gccgccaagg agcgcccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 388 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 388 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Glu Arg Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 389 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 389 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaagg agaagccggc tccggctggt 1200 gcaccgcaac gtactacg 1218 <210> 390 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 390 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Glu Lys Pro Ala Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 391 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 391 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 392 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 392 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 393 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 393 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 394 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 394 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 395 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 395 atggggaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 396 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 396 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 397 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 397 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 398 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 398 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 399 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 399 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 400 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 400 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 401 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 401 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 402 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 402 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 403 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 403 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcggcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 404 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 404 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 405 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 405 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggtccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 406 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 406 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 407 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 407 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 408 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 408 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 409 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 409 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct tttaccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 410 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 410 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Leu 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 411 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 411 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccaatgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gcttcggcac cgagcgatac ggccaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 412 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 412 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Met Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 413 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 413 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgacgaa 1140 gctgcggcac cgagcgatac ggccaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 414 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 414 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 415 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 415 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcgccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggctact 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataatgcg 1140 gctgccgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 416 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 416 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Ala Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Gln Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Thr Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asn Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 417 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 417 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 418 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 418 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 419 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 419 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcgtccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgacgaa 1140 gctgcggcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 420 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 420 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 421 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 421 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcgacatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 422 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 422 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Asp Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 423 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 423 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcggcac cgagcgatac gcagggtccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 424 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 424 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 425 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 425 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccaatgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac ggccaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 426 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 426 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Met Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 427 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 427 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcgtccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 428 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 428 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 429 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 429 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac ggccaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 430 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 430 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 431 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 431 atggagaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccggtccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 432 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 432 Met Glu Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 433 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 433 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcgtccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 434 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 434 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Gly 385 <210> 435 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 435 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccaatgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgacgaa 1140 gcttcggcac cgagcgatac ggccaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 436 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 436 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Met Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 437 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 437 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gcttcggcac cgagcgatac ggccggtccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 438 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 438 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Gly Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 439 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 439 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 440 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 440 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 441 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 441 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcggcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 442 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 442 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 443 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 443 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 444 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 444 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 445 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 445 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactttt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 446 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 446 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 447 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 447 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccaatgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac ggccggttga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 448 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 448 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Met Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Gly 385 <210> 449 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 449 atgggtaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcgtccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 450 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 450 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 451 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 451 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg cgtccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aacattatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gctgcggcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 452 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 452 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ala 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys His Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 453 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 453 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagca 1140 gcttcggcac cgagcgatac gcagaagtga gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 454 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 454 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ser Ala Pro 370 375 380 Ser Asp Thr Gln Lys 385 <210> 455 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 455 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 456 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 456 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 457 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 457 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 458 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 458 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 459 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 459 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 460 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 460 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 461 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 461 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcgtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcggcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 462 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 462 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 463 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 463 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 464 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 464 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 465 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 465 atggggatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg agtccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcacta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac gcagaagccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 466 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 466 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 467 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 467 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggctact 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataatgcg 1140 gctgccgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 468 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 468 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Thr Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asn Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 469 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 469 atggggaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 470 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 470 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 471 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 471 atgggtaatc tgaaatttcc agatggcgcg ttgggcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgcagcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 472 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 472 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Gly Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 473 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 473 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgctcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg cttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgag 1140 gctgccgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 474 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 474 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 475 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 475 atgggtaatc tgaaatttcc agatggcgcg ttgggcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atgtgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaagatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg cttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaaagt 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagcg 1140 gctgccgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 476 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 476 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Gly Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Val Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Ala Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 477 <211> 1218 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 477 atggggaacc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaaagt 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgaggcg 1140 gctggcgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacg 1218 <210> 478 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 478 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Glu Ala Ala Gly Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 479 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 479 atggggaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgctcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaaact 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgag 1140 gctggcgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 480 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 480 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Thr Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Gly Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 481 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 481 atggggaatc tgaaatttcc agatggcgcg ttgggcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgctg aatccagtta tcgcgtgcat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atgtgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg cttgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcaaagt 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaggcg 1140 gctggcgcac cgagcgatac gcagaaaccc gccgccaaga aacgcccgac accggctggt 1200 gcaccgcaac gtactacgta g 1221 <210> 482 <211> 406 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 482 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Gly Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Glu 50 55 60 Ser Ser Tyr Arg Val His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Val Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Ala Ala Gly Ala Pro 370 375 380 Ser Asp Thr Gln Lys Pro Ala Ala Lys Lys Arg Pro Thr Pro Ala Gly 385 390 395 400 Ala Pro Gln Arg Thr Thr 405 <210> 483 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 483 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 484 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 484 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 485 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 485 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 486 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 486 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 487 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 487 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 488 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 488 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 489 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 489 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 490 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 490 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 491 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 491 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcctca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 492 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 492 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 493 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 493 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 494 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 494 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 495 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 495 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 496 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 496 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 497 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 497 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaggaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 498 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 498 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 499 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 499 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 500 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 500 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 501 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 501 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcagcac cgagcgatac ggccaag 1167 <210> 502 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 502 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 503 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 503 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaggaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 504 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 504 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 505 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 505 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcctca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcagcac cgagcgatac ggccaag 1167 <210> 506 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 506 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 507 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 507 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 508 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 508 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 509 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 509 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 510 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 510 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 511 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 511 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 512 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 512 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 513 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 513 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 514 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 514 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 515 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 515 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 516 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 516 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 517 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 517 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 518 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 518 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 519 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 519 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 520 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 520 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 521 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 521 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 522 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 522 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 523 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 523 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcctca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 524 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 524 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 525 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 525 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaggaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 526 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 526 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 527 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 527 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 528 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 528 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 529 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 529 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 530 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 530 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 531 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 531 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 532 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 532 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 533 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 533 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 534 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 534 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 535 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 535 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caatgatgaa 1140 gctgcagcac cgagcgatac ggccaag 1167 <210> 536 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 536 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Asp Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 537 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 537 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 538 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 538 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 539 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 539 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcctca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 540 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 540 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 541 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 541 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 542 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 542 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 543 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 543 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctcgcagca ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaggaa 1140 gcttcggcac cgagcgatac ggccaag 1167 <210> 544 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 544 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ser Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 545 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 545 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgcctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctcgcacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcgt cgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccggcctca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 546 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 546 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Ala Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 547 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 547 atgggtatgc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgc tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgc gctgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 548 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 548 Met Gly Met Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Leu Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Arg 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 549 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 549 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaatgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcattggt 960 gtagaagcga tgcgtaatcc aaacgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 550 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 550 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 551 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 551 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgcg tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaatgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcattggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtgagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gccgcggcac cgagcgatac ggccaag 1167 <210> 552 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 552 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met Arg Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Glu His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 553 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 553 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcattggt 960 gtagaagcga tgcgtaatcc aaacgccatt accgttgtgt tcccgaaacc ttcgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 554 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 554 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Ser Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 555 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 555 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcattggt 960 gtagaagcga tgcgtaatcc aaacgccatt accgttgtgt tcccgaaacc ttcgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtgagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 556 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 556 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Ser Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Glu His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 557 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 557 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggtga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaatgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 558 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 558 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 559 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 559 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggtga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcgcaggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 560 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 560 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ala Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 561 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 561 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgcg tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc aaacgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtgagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gccgcggcac cgagcgatac ggccaag 1167 <210> 562 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 562 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met Arg Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Glu His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 563 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 563 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtgagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 564 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 564 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Glu His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 565 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 565 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taaaccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaatgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcattggt 960 gtagaagcga tgcgtaatcc aaacgccatt accgttgtgt tcccgaaacc ttcgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gccgcggcac cgagcgatac ggccaag 1167 <210> 566 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 566 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Ser Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 567 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 567 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgcg tattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaatgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gccgcggcac cgagcgatac ggccaag 1167 <210> 568 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 568 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met Arg Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 569 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 569 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctgggagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 570 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 570 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Gly Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 571 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 571 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 cgggaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 572 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 572 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Arg Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 573 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 573 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagactgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 574 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 574 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Thr Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 575 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 575 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcaggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 576 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 576 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Gln Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 577 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 577 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaaaagtact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 578 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 578 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Lys Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 579 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 579 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 gttgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 580 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 580 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Val Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 581 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 581 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgc tgaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 582 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 582 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Leu Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 583 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 583 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgttttgta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 584 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 584 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Cys Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 585 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 585 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttgggt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 586 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 586 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Trp 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 587 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 587 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgctga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 588 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 588 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Ala Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 589 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 589 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 590 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 590 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 591 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 591 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctgatagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 592 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 592 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Asp Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 593 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 593 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 594 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 594 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 595 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 595 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcatagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 596 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 596 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala His Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 597 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 597 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt gtaagaaact ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 598 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 598 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Cys Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 599 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 599 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagcttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 600 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 600 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Ala Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 601 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 601 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgcggatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 602 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 602 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Ala 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 603 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 603 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaaact ggcgattcac 600 gattctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 604 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 604 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Asp Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 605 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 605 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccagcg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 606 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 606 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 607 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 607 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcttgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 608 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 608 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Leu Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 609 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 609 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gcttctagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 610 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 610 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Ser Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 611 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 611 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 612 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 612 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 613 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 613 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcaggttgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 614 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 614 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Val Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 615 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 615 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 616 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 616 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 617 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 617 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 aaggaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 618 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 618 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Lys Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 619 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 619 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgggt 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 620 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 620 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Gly 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 621 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 621 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gcggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 622 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 622 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Ala Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 623 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 623 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacgtt 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 624 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 624 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Val Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 625 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 625 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcaggctgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 626 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 626 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Ala Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 627 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 627 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacagtga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 628 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 628 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Ser Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 629 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 629 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgctt ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 630 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 630 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Leu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 631 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 631 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca caggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 632 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 632 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Gln Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 633 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 633 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctattagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 634 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 634 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Ile Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 635 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 635 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgacgttcg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctcgcagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 636 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 636 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Val 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Arg Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 637 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 637 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 638 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 638 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 639 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 639 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgttttgta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 cgcgaaggca ttgacaacgt acgcaccatc caggatatgt gcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 640 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 640 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Cys Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Arg Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Cys Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 641 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 641 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggca 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 642 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 642 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 643 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 643 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 cgcgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 644 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 644 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Arg Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 645 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 645 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcatgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 646 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 646 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 647 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 647 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 actgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 648 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 648 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 649 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 649 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggca 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 650 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 650 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 651 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 651 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 652 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 652 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 653 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 653 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 654 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 654 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 655 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 655 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggca 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 656 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 656 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 657 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 657 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 658 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 658 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 659 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 659 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgcccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctactacga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcacgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 660 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 660 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Thr Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 661 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 661 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga ttgtccgccg atcatcttcg ctaacattgg tacgacgatg 540 actgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgcgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 662 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 662 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Cys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 663 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 663 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 664 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 664 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 665 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 665 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcacgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 666 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 666 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 667 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 667 atgggtaatc tgaaatttcc agacggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 668 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 668 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 669 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 669 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgttttgta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 670 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 670 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Cys Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 671 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 671 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggca 240 tggttcgccg aactcatgca cattgcacca gcggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 672 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 672 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Ala Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 673 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 673 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgttttgta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc aaatgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 674 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 674 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Cys Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Asn Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 675 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 675 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacgtc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 676 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 676 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 677 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 677 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 678 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 678 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 679 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 679 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgtatt 780 gctactagta ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagcacgt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 680 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 680 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln His Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 681 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 681 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcta gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 682 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 682 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 683 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 683 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccgccg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaggaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagactc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagcgcatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 684 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 684 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Arg 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Arg Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 685 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 685 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 686 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 686 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 687 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 687 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 688 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 688 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 689 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 689 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaacgt 900 cgtgttcagg catgtctgga taacgcccgc tatacggtcg agcaatttca gcgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 690 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 690 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Gln Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Gln Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 691 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 691 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatcg tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct gggtattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 692 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 692 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Val 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 693 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 693 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc cagtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 694 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 694 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 695 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 695 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattccacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 696 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 696 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Pro Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 697 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 697 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagcta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 698 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 698 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 699 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 699 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagcta tcgcatccat acccgtgaaa tcgaagtcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct gggtattagt 600 cgctctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 700 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 700 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Val Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Arg Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 701 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 701 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaagtcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattcat 600 cgctctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 702 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 702 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Val Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Arg Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 703 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 703 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagcta tcgcatccat acccgtgaaa tcgaagtcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 704 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 704 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Val Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 705 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 705 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc taccggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggctattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcaatttaa gcgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 706 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 706 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 707 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 707 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaagtcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc taccggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct gggtattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaacgt 900 cgtgttcagg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 708 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 708 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Val Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Gln Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 709 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 709 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaagtcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc taccggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct gggtattagt 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 710 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 710 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Val Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 711 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 711 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 712 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 712 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 713 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 713 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gattctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgctgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 714 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 714 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Asp Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Ala 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 715 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 715 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgcgga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 716 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 716 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Ala Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 717 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 717 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gattctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 718 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 718 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Asp Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 719 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 719 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gattctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 720 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 720 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Asp Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 721 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 721 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 722 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 722 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 723 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 723 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaaacttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 724 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 724 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 725 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 725 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacatgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgctgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 726 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 726 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Met Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Ala 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 727 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 727 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctagcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 728 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 728 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 729 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 729 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgcgga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 730 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 730 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Ala Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 731 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 731 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gattctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 732 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 732 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Asp Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 733 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 733 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttacgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga gccgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 734 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 734 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Ser Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 735 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 735 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctgaag 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacgggga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgt gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgatgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 736 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 736 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Lys 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 737 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 737 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 738 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 738 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 739 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 739 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataagt ctctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 740 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 740 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Ser Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 741 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 741 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcactg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 742 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 742 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Thr Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 743 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 743 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggct atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 744 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 744 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 745 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 745 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctacatgt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 746 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 746 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Met Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 747 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 747 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaactt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 748 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 748 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Leu Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 749 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 749 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tctgggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 750 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 750 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 751 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 751 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcattg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 752 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 752 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Ile Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 753 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 753 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtgggttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 754 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 754 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 755 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 755 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataagg cgctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 756 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 756 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Ala Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 757 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 757 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatgcggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 758 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 758 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 759 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 759 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctacgttt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 760 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 760 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Val Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 761 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 761 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtttgttt ttagcgcgtg aacgctttcc tacgggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcgcgg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt acgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta gcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgatgg cttcaaacgt 900 cgtgttgcgg catgtctgga taacgcccgc tatacggtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 762 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 762 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Leu Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Ala Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ala Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 763 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 763 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgcca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 764 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 764 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 765 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 765 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 766 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 766 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 767 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 767 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacgtct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct gggtattcat 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 768 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 768 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Val Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 769 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 769 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatcattg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 770 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 770 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Ile Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 771 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 771 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgcca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcat 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 772 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 772 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 773 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 773 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccgccg atcatcttcg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct gggtattagt 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 774 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 774 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Pro Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 775 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 775 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtctggaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctactatt cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 776 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 776 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Leu Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Tyr Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 777 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 777 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacgtct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct gggtattagt 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 778 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 778 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Val Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile Ser Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 779 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 779 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacgtct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cctcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct gggtattcat 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccctg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tataccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 780 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 780 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Val Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Pro His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Gly Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Leu Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Thr Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 781 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 781 atgggtaatc tggtctttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 gactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca tcgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacaccacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 782 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 782 Met Gly Asn Leu Val Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met Asp Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 783 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 783 atgggtaatc tggtctttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 gactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca tcgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cgatggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacaccacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 784 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 784 Met Gly Asn Leu Val Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met Asp Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Asp Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 785 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 785 atgggtaatc tgaagtttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 gactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca tcgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacaccacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 786 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 786 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met Asp Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 787 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 787 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt gtggttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 788 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 788 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Val Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 789 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 789 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacattg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 790 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 790 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Ile Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 791 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 791 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccattaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 792 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 792 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Ile Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 793 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 793 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa attccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 794 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 794 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Asn 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 795 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 795 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacaccttt ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 796 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 796 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Phe Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 797 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 797 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc gaggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 798 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 798 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Glu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 799 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 799 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgaccttg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 800 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 800 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Leu Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 801 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 801 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc caggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 802 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 802 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Gln Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 803 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 803 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcat ggagcgatac ggccaag 1167 <210> 804 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 804 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Trp 370 375 380 Ser Asp Thr Ala Lys 385 <210> 805 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 805 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttc gttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 806 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 806 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Ser Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 807 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 807 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatattt gggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 808 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 808 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Leu Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 809 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 809 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagcttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 810 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 810 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Ala Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 811 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 811 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccctta tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 812 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 812 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Leu Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 813 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 813 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaaacttc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 814 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 814 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Thr Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 815 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 815 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccacg gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 816 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 816 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Thr Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 817 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 817 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtaccgct gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 818 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 818 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Ala Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 819 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 819 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttccgtatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 820 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 820 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Pro Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 821 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 821 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaacta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgatggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaagg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgagg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 822 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 822 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Glu Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 823 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 823 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 824 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 824 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 825 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 825 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 826 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 826 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 827 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 827 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 828 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 828 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 829 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 829 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 830 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 830 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 831 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 831 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 832 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 832 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 833 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 833 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 834 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 834 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 835 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 835 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 836 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 836 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 837 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 837 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 838 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 838 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 839 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 839 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 840 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 840 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 841 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 841 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 842 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 842 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 843 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 843 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tgacggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 844 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 844 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Asp Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 845 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 845 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaaaag 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 846 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 846 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Lys Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 847 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 847 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 848 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 848 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 849 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 849 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccagtta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgaggg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 850 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 850 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Ser Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Glu Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385 <210> 851 <211> 1167 <212> DNA <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 851 atgggtaatc tgaaatttcc agatggcgcg ttgagcccga ccgaccaacg ccgtattgaa 60 gatgtgtatc agtacgttcg tgagcaatgc gacaccttca tcgggtatcc gtgcaacatg 120 cactttgatt acacacctct ttttccgttc ctggcctatc ccgtcaacaa cgtgggcgat 180 ccgtttgcaa gctccaatta tcgcatccat acccgtgaaa tcgaacgcga ggttctggcg 240 tggttcgccg aactcatgca cattgcacca gaagaatact ggggttatgt gactaatgga 300 ggatcggagg gcaacctgta cggtggcttt ttagcgcgtg aacgctttcc tactggcgtc 360 gcctacttct cggaagattc gcattatagc gtgtataaga atctgcgtct gctgaacatg 420 cgtcacatca tgatcaaatc acagccgaat ggtgagattg attacggcga tctctacgaa 480 actatgcgcg ctcatcgtga taagccggca atcatctttg ctaacattgg tacgacgatg 540 accgaaggca ttgacaacgt gcgcaccatc caggatatgt tcaagaagct ggcgattcac 600 gaatctcata tccattgtga cgcagcatta tcggggatga ccctgccctt tatggatgat 660 gccccagcgt ttgactttgc cactggcatt gattcgctta gcatctccgg tcacaaattc 720 attgggtctc cgatgccttg cggcatcgtt ttgacacgca aaccttatgt ggagaaaatt 780 gctacttcga ttgaatatat cggtacccgc gacactacga tttcgggcag ccgtaatggt 840 attacaccgc tgtttctttg gtacgcggtg aaacaagctg gacgcgacgg cttcaaacgc 900 cgtgttgcgg catgtctgga taacgcccgc tatgccgtcg agcagtttaa acgcatcggt 960 gtagaagcga tgcgtaatcc atgtgccatt accgttgtgt tcccgaaacc tccgcagtca 1020 gtgctggaaa agtggcaaat cgcaaccgag ggcgacgatg cgcatatttg cttaatgcca 1080 agtaccaccc gtcagatggt cgatgaattg atcgctgaca ttgcggccgc caataaagaa 1140 gctgcggcac cgagcgatac ggccaag 1167 <210> 852 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Variant of Leucine Decarboxylase from Planctomycetaceae bacterium <400> 852 Met Gly Asn Leu Lys Phe Pro Asp Gly Ala Leu Ser Pro Thr Asp Gln 1 5 10 15 Arg Arg Ile Glu Asp Val Tyr Gln Tyr Val Arg Glu Gln Cys Asp Thr 20 25 30 Phe Ile Gly Tyr Pro Cys Asn Met His Phe Asp Tyr Thr Pro Leu Phe 35 40 45 Pro Phe Leu Ala Tyr Pro Val Asn Asn Val Gly Asp Pro Phe Ala Ser 50 55 60 Ser Asn Tyr Arg Ile His Thr Arg Glu Ile Glu Arg Glu Val Leu Ala 65 70 75 80 Trp Phe Ala Glu Leu Met His Ile Ala Pro Glu Glu Tyr Trp Gly Tyr 85 90 95 Val Thr Asn Gly Gly Ser Glu Gly Asn Leu Tyr Gly Gly Phe Leu Ala 100 105 110 Arg Glu Arg Phe Pro Thr Gly Val Ala Tyr Phe Ser Glu Asp Ser His 115 120 125 Tyr Ser Val Tyr Lys Asn Leu Arg Leu Leu Asn Met Arg His Ile Met 130 135 140 Ile Lys Ser Gln Pro Asn Gly Glu Ile Asp Tyr Gly Asp Leu Tyr Glu 145 150 155 160 Thr Met Arg Ala His Arg Asp Lys Pro Ala Ile Ile Phe Ala Asn Ile 165 170 175 Gly Thr Thr Met Thr Glu Gly Ile Asp Asn Val Arg Thr Ile Gln Asp 180 185 190 Met Phe Lys Lys Leu Ala Ile His Glu Ser His Ile His Cys Asp Ala 195 200 205 Ala Leu Ser Gly Met Thr Leu Pro Phe Met Asp Asp Ala Pro Ala Phe 210 215 220 Asp Phe Ala Thr Gly Ile Asp Ser Leu Ser Ile Ser Gly His Lys Phe 225 230 235 240 Ile Gly Ser Pro Met Pro Cys Gly Ile Val Leu Thr Arg Lys Pro Tyr 245 250 255 Val Glu Lys Ile Ala Thr Ser Ile Glu Tyr Ile Gly Thr Arg Asp Thr 260 265 270 Thr Ile Ser Gly Ser Arg Asn Gly Ile Thr Pro Leu Phe Leu Trp Tyr 275 280 285 Ala Val Lys Gln Ala Gly Arg Asp Gly Phe Lys Arg Arg Val Ala Ala 290 295 300 Cys Leu Asp Asn Ala Arg Tyr Ala Val Glu Gln Phe Lys Arg Ile Gly 305 310 315 320 Val Glu Ala Met Arg Asn Pro Cys Ala Ile Thr Val Val Phe Pro Lys 325 330 335 Pro Pro Gln Ser Val Leu Glu Lys Trp Gln Ile Ala Thr Glu Gly Asp 340 345 350 Asp Ala His Ile Cys Leu Met Pro Ser Thr Thr Arg Gln Met Val Asp 355 360 365 Glu Leu Ile Ala Asp Ile Ala Ala Ala Asn Lys Glu Ala Ala Ala Pro 370 375 380 Ser Asp Thr Ala Lys 385

Claims (62)

  1. 조작된 류신 데카르복실라제 폴리펩타이드로서, 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766 중 적어도 하나에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 아미노산 서열을 포함하고, 여기서 상기 아미노산 서열의 아미노산 위치는 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688, 및/또는 766의 아미노산 서열을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  2. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 2에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  3. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 4에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  4. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 6에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  5. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 8에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  6. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 10에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  7. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 14에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  8. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 12에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00088

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 12를 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  9. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 38에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00089

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 38을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  10. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 234에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00090

    Figure pct00091

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 234를 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  11. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 284에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00092

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 284를 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  12. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 484에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00093

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 484를 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  13. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 594에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00094

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 594를 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  14. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 686에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00095

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  15. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 686에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치 19, 109, 123, 134, 170, 173, 187, 211, 및 312로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 686을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  16. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 688에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치
    Figure pct00096

    Figure pct00097

    로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 688을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  17. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 766에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치 5/41, 5/41/228, 33, 41, 47, 51, 55, 64, 126, 265, 267, 270, 331, 353, 357, 및 384로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  18. 제1항에 있어서, 상기 폴리펩타이드 서열이 서열 번호 766에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과의 서열 동일성을 갖고, 상기 조작된 류신 데카르복실라제 폴리펩타이드의 폴리펩타이드 서열이 위치 66, 66/118, 66/118/296, 66/118/296/300, 66/118/300, 66/296, 66/296/300, 66/300, 118, 118/296, 118/296/300, 118/300, 296, 296/300, 및 300으로부터 선택되는 하나 이상의 아미노산 위치에 적어도 하나의 치환 또는 치환 세트를 포함하고, 여기서 아미노산 위치는 서열 번호 766을 기준으로 하여 넘버링되는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  19. 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688 및/또는 766에 대해 적어도 90%의 서열 동일성을 갖는 아미노산 서열을 포함하는 조작된 류신 데카르복실라제 폴리펩타이드.
  20. 제1항 내지 제19항 중 어느 한 항에 제시된 조작된 폴리펩타이드를 포함하는 조작된 류신 데카르복실라제 폴리펩타이드.
  21. 제20항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 서열 번호 2, 4, 6, 8, 10, 12, 14, 38, 234, 284, 484, 594, 686, 688 및/또는 766에 대해 적어도 약 95%의 서열 동일성을 갖는 아미노산 서열을 포함하는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  22. 제1항 내지 제18항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 표 1-2, 2-1, 3-2, 4-1, 5-1, 6-1, 7-1, 8-1, 8-2, 10-1, 11-1 및/또는 11-2 중 어느 하나에 제시된 변이체 류신 데카르복실라제 폴리펩타이드인 조작된 류신 데카르복실라제 폴리펩타이드.
  23. 제1항 내지 제22항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 플랑크토마이세타세아에(Planctomycetaceae) 변이체 효소인 조작된 류신 데카르복실라제 폴리펩타이드.
  24. 제1항 내지 제23항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 야생형 플랑크토마이세타세아에 종 류신 데카르복실라제보다 류신에 대해 더 많은 활성을 나타내는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  25. 제1항 내지 제24항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 야생형 플랑크토마이세타세아에 종 류신 데카르복실라제보다 단백질 분해에 대해 내성이 더 높은 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  26. 제1항 내지 제25항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 야생형 플랑크토마이세타세아에 종 류신 데카르복실라제보다 열안정성이 더 높은 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  27. 제1항 내지 제26항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 서열 번호 16-852의 임의의 짝수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과로 동일한 서열을 포함하는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  28. 제27항에 있어서, 상기 류신 데카르복실라제 폴리펩타이드가 서열 번호 16-852의 임의의 짝수 서열에 대해 적어도 90% 동일한 서열을 포함하는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  29. 제28항에 있어서, 상기 류신 데카르복실라제 폴리펩타이드가 서열 번호 16-852의 임의의 짝수 서열을 포함하는 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  30. 제1항 내지 제29항 중 어느 한 항에 있어서, 상기 조작된 류신 데카르복실라제 폴리펩타이드가 정제된 것인 조작된 류신 데카르복실라제 폴리펩타이드.
  31. 제1항 내지 제30항 중 어느 한 항에 제시된 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 조성물.
  32. 제1항 내지 제31항 중 어느 한 항의 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 조작된 폴리뉴클레오타이드 서열.
  33. 제32항에 있어서, 상기 폴리뉴클레오타이드 서열이 서열 번호 15-851의 임의의 홀수 서열에 대해 적어도 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 또는 그 초과로 동일한 서열을 포함하는 것인 조작된 폴리뉴클레오타이드 서열.
  34. 제32항에 있어서, 상기 폴리뉴클레오타이드 서열이 서열 번호 15-851의 임의의 홀수 서열에 대해 적어도 90% 또는 그 초과로 동일한 서열을 포함하는 것인 조작된 폴리뉴클레오타이드 서열.
  35. 제32항에 있어서, 상기 폴리뉴클레오타이드 서열이 서열 번호 15-851의 임의의 홀수 서열을 포함하는 것인 조작된 폴리뉴클레오타이드 서열.
  36. 제32항 내지 제35항 중 어느 한 항에 있어서, 상기 서열이 제어 서열에 작동 가능하게 연결되는 것인 조작된 폴리뉴클레오타이드 서열.
  37. 제32항 내지 제36항 중 어느 한 항에 있어서, 상기 폴리뉴클레오타이드가 코돈 최적화된 것인 조작된 폴리뉴클레오타이드 서열.
  38. 제32항 내지 제37항 중 어느 한 항의 적어도 하나의 조작된 폴리뉴클레오타이드 서열, 및 적어도 하나의 제어 서열을 포함하는 발현 벡터.
  39. 제38항에 있어서, 상기 제어 서열이 프로모터를 포함하는 것인 발현 벡터.
  40. 제39항에 있어서, 상기 프로모터가 이종 프로모터인 발현 벡터.
  41. 제32항 내지 제37항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열로 형질전환되고/되거나, 제38항 내지 제40항 중 어느 한 항의 발현 벡터를 포함하는 숙주 세포.
  42. 제41항에 있어서, 이. 콜라이(E. coli)인 숙주 세포.
  43. 제1항 내지 제30항 중 어느 한 항의 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드 및/또는 제32항 내지 제37항 중 어느 한 항의 적어도 하나의 폴리뉴클레오타이드 서열 및/또는 제38항 내지 제40항 중 어느 한 항의 적어도 하나의 발현 벡터를 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함하는, 숙주 세포에서 조작된 류신 데카르복실라제 폴리펩타이드의 생산 방법.
  44. 제43항에 있어서, 제1항 내지 제30항 중 어느 한 항의 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 코딩하는 적어도 하나의 폴리뉴클레오타이드를 포함하는 숙주 세포를 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드가 생성되도록 하기에 적합한 배양 조건 하에 배양하는 것을 포함하는 방법.
  45. 제43항 또는 제44항에 있어서, 배양액 및/또는 숙주 세포로부터 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 회수하는 단계를 추가로 포함하는 방법.
  46. 제43항 내지 제45항 중 어느 한 항에 있어서, 상기 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 정제하는 단계를 추가로 포함하는 방법.
  47. 제32항 내지 제37항 중 어느 한 항의 적어도 하나의 조작된 폴리뉴클레오타이드를 포함하는 조성물.
  48. 제47항에 있어서, 약학 조성물인 조성물.
  49. 제48항에 있어서, 적어도 하나의 약학적으로 허용되는 부형제 및/또는 담체를 추가로 포함하는 조성물.
  50. 제31항, 제48항 및 제49항 중 어느 한 항에 있어서, 메이플 시럽 요증의 치료에 적합한 조성물.
  51. 제31항 및 제47항 내지 제50항 중 어느 한 항에 있어서, 인간에 대한 경구 투여에 적합한 조성물.
  52. 제31항 및 제47항 내지 제51항 중 어느 한 항에 있어서, 환제, 정제, 캡슐, 젤캡, 액체 또는 에멀젼의 형태인 조성물.
  53. 제52항에 있어서, 상기 환제, 정제, 캡슐 또는 젤캡이 장용 코팅을 추가로 포함하는 것인 조성물.
  54. 제31항 및 제47항 내지 제52항 중 어느 한 항에 있어서, 인간에 대한 비경구 주사에 적합한 조성물.
  55. 제31항 및 제47항 내지 제54항 중 어느 한 항에 있어서, 적어도 하나의 추가의 치료상 효과적인 화합물과 공동으로 투여되는 조성물.
  56. 제55항에 있어서, 적어도 하나의 추가의 치료상 효과적인 화합물을 포함하는 조성물.
  57. 메이플 시럽 요증이 있는 대상체를 제공하는 단계, 및 제31항 및 제47항 내지 제56항 중 어느 한 항의 조성물을 상기 대상체에게 제공하는 단계를 포함하는, 대상체에서 메이플 시럽 요증의 증상을 치료 및/또는 예방하는 방법.
  58. 제57항에 있어서, 상기 메이플 시럽 요증의 증상이 개선되는 것인 방법.
  59. 제57항 또는 제58항에 있어서, 상기 대상체가, 제1항 내지 제30항 중 어느 한 항에 제시된 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 적어도 하나의 조성물이 제공되지 않은 대상체에 의해 요구되는 식이보다 이소류신, 류신 및/또는 발린 함량이 덜 제한된 식이를 먹을 수 있는 것인 방법.
  60. 제57항 내지 제59항 중 어느 한 항에 있어서, 상기 대상체가, 제43항 내지 제46항 중 어느 한 항에 제시된 방법을 사용하여 생산된 적어도 하나의 조작된 류신 데카르복실라제 폴리펩타이드를 포함하는 적어도 하나의 조성물이 제공되지 않은 대상체에 의해 요구되는 식이보다 이소류신, 류신 및/또는 발린 함량이 덜 제한된 식이를 먹을 수 있는 것인 방법.
  61. 제57항 내지 제60항 중 어느 한 항에 있어서, 상기 대상체가 유아, 아동, 청소년, 또는 성인인 방법.
  62. 제31항 및 제47항 내지 제56항 중 어느 한 항에서 제공되는 조성물의 용도.
KR1020227030236A 2020-02-04 2021-02-03 조작된 류신 데카르복실라제 KR20220137057A (ko)

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