KR20220083535A - Composition for improving companion animal heart function and functional food containing the same - Google Patents

Abstract

The present invention relates to a composition for improving heart function in companion animals and a functional food containing the same.
The composition for improving heart function in companion animals according to the present invention includes colostrum powder, L-carnitine and coenzyme Q10 as active ingredients.
According to the above composition, the composition for improving heart function in companion animals according to the present invention is effective with colostrum powder that helps to enhance immunity and strengthens heart muscle, and L-carnitine and coenzyme Q10 that help supply heart energy and protect heart cells. By containing it as an ingredient, it can be fed regularly over a certain period of time to prevent heart disease in companion animals and help improve heart function.

Description

Composition for improving heart function in companion animals and functional food containing the same

The present invention relates to a composition for improving the heart function of companion animals and a functional food containing the same, and more particularly, colostrum powder that helps to enhance immunity and strengthens heart muscle, and L that helps supply heart energy and protect heart cells - A composition for improving heart function in companion animals that can prevent heart disease in companion animals and help improve heart function by providing a composition containing carnitine and coenzyme Q10 as active ingredients for a certain period of time or longer It relates to a functional food comprising.

Interest in companion animals is increasing as the number of people living alone increases due to the rapid change in family type to nuclear family. It is being released in a variety of ways.

For example, the concept of companion animals has been changed according to the trend of becoming more and more common in an environment where people feel like a family by not only providing medical benefits to companion animals but also funerals in the event of death.

In other words, from the concept of animals bred for pleasure by humans, the perception of animals that live together with humans has changed, meaning that they are not toys for humans by respecting the various benefits that animals provide to humans. , it can be said that it clearly shows the relationship between humans and animals in modern society.

Meanwhile, as the lifespan of companion animals has increased recently, the importance of heart health, which directly affects the lifespan, is being emphasized. The heart is an important organ responsible for blood circulation throughout the body, and as it is the most central organ for all animals, any problems can be life-threatening.

For example, a dog's heart is composed of a 2 atrium and 2 ventricle structure just like a human's. In general, heart disease in dogs is a problem with the valve that prevents backflow of the heart (myxomatous cuspid disease), and the blood flow path is narrow. heart disease (subaortic stenosis and valvular stenosis), problems with the myocardium itself (dilated or hypertrophic cardiomyopathy), presence of a hole in the heart (atrial septal defect, ventricular septal defect), problems with the blood vessels connecting the aorta to the pulmonary artery (arteriosclerosis) respect), and the most common disease is a disease caused by a valve problem. If there is a problem with the valves, the blood flows back and the blood leaks out at a high speed into the narrow space between the valves, causing vibration and noise.

There is a valve between the heart and ventricle, which plays an important role in circulating blood. Among them, the valve between the left atrium and left ventricle is called the bicuspid valve. Bicuspid occlusive insufficiency accounts for the majority of heart diseases in elderly dogs, and it is particularly common in small dogs weighing less than 5 kg and older dogs aged 7 to 8 years old.

These early symptoms of heart disease are difficult to distinguish from changes caused by aging, such as deterioration of physical condition, fatigue, and loss of appetite. For accurate diagnosis of heart disease, various tests such as radiographic examination, echocardiography, x-ray, electrocardiogram, blood pressure and blood test, kidney level, and cardiovascular angiography are required. It can be said that early symptom detection and continuous management are very important because the death rate is high if left untreated.

Although heart disease in dogs cannot be completely cured as a progressive disease, symptoms can be alleviated and the disease progression can be slowed through medication, diet, and exercise.

Heart-related medicines prescribed for heart disease are used for the quality of life of dogs by relieving clinical symptoms such as cough, shortness of breath, and loss of energy while slowing the progression of the disease. However, the fact that it has to be paid continuously is a big problem in that it has a large cost and side effects.

In particular, in the case of diuretics, there is a problem that can cause kidney failure due to damage to kidney cells during long-term feeding, from body tremors due to electrolyte imbalance even with short-term use. While it is reported that it can be delayed, various side effects such as anorexia and gastrointestinal disorders such as diarrhea, dyspnea, and azotemia have been reported, from reports that it induces arrhythmias related to heart disease, so caution and restrictions on its use follow.

When the heart disease is classified into stages 1 to 4 according to the degree of progression (NYHA Functional Classfication), the medicines prescribed for basic heart disease are divided into basic medicines such as diuretics, cardiac drugs, and blood pressure regulators (ACE inhibitors) and other medicines according to the degree of progression. Sequential addition is the basis of drug use, and these drugs usually have clinical symptoms such as cough and shortness of breath, along with cardiac hypertrophy, pulmonary edema, pericardial fluid, ascites, etc. The basic principle is to use it from the time it appears during daily activities), and it is difficult to apply to the first and second stages requiring initial management due to various side effects accompanying long-term feeding.

On the other hand, generally recommended methods for heart health management include limiting salt intake and moderate exercise. The European Federation of Pet Food Industry Federation (FEDIAF) guidelines for sodium levels for healthy adult dogs are 0.10 to 1.80% (based on DM), but dogs with heart disease may benefit from a diet low in sodium and to reduce the risk of early heart disease. As a general guideline, the ideal diet should contain less than 0.4% sodium of the dry matter (DM) standard, and should be reduced to less than 0.3% (DM) when clinical symptoms appear. However, sodium limits depend on the stage of heart disease, and the exact number remains unclear.

In addition, it is difficult to limit salt intake continuously due to an increase in the possibility of exposure to highly palatable foods (including salt) to dogs, who have anorexia and become more picky as they get older with a high rate of heart disease Lack of exercise can make your heart health worse.

Therefore, there is a need for a more sustainable heart health management method, and it is necessary to develop a functional food for improving the heart function of companion animals with high palatability so that it can be consumed for a long time without side effects through nutritional supplementation of safe food ingredients that help prevent and improve heart disease. This is an urgent situation.

Domestic Registered Patent No. 10-2099464 (registered on April 03, 2020) Domestic Registered Patent No. 10-1035758 (Registered on May 12, 2011) Domestic Registered Patent No. 10-1694780 (Registered on January 04, 2017)

The present invention provides a composition containing colostrum powder, which helps to enhance immunity and strengthens heart muscle, and L-carnitine and coenzyme Q10, which helps to supply heart energy and protect heart cells, as active ingredients, An object of the present invention is to provide a composition for improving the heart function of companion animals that can help prevent heart disease in companion animals and help improve heart function by feeding and a functional food containing the same.

In addition, the present invention provides a composition for improving the heart function of companion animals that can be safely ingested without side effects even for long-term feeding, even for companion animals that are diseased or unhealthy, with high convenience and palatability, and can stably supply balanced nutritional ingredients, and including the same to provide functional foods that

Various problems to be solved by the present invention are not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The composition for improving heart function in companion animals according to the present invention includes colostrum powder, L-carnitine and coenzyme Q10 as active ingredients.

It may be included in a weight ratio of 2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, and 0.1 to 3 parts by weight of coenzyme Q10.

In addition to the colostrum powder, L-carnitine and coenzyme Q10, meat protein, potato starch, tapioca starch, soybean powder, trehalose, fructooligosaccharide, vegetable glycerin, olive oil, taurine, chitosan, omega-3 fatty acid, L-arginine, vitamin C and vitamin E.

2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, 0.1 to 3 parts by weight of coenzyme Q10, 25 to 35 parts by weight of meat protein, 12 to 18 parts by weight of potato starch, 20 to 25 parts by weight of tapioca starch, Soybean powder 5 to 10 parts by weight, trehalose 2 to 6 parts by weight, fructooligosaccharide 3 to 7 parts by weight, vegetable glycerin 2 to 6 parts by weight, olive oil 3 to 7 parts by weight, taurine 0.1 to 1 parts by weight, chitosan 0.1 to 1 It may be included in a weight ratio of 0.1 to 3 parts by weight of omega-3 fatty acids, 0.5 to 1.5 parts by weight of L-arginine, 0.1 to 1 parts by weight of vitamin C, and 0.1 to 1 parts by weight of vitamin E.

As the colostrum powder, bovine colostrum extract powder may be used, and duck meat may be used as the meat protein.

In addition, the present invention discloses a functional food comprising the composition described above.

Specific details of other embodiments are included in the detailed description.

The composition for improving heart function in companion animals according to the present invention contains, as active ingredients, colostrum powder, which helps to enhance immunity and strengthens the heart muscle, and L-carnitine and coenzyme Q10, which help to supply heart energy and protect heart cells, By feeding it regularly over a certain period of time, it can help prevent heart disease in companion animals and improve heart function.

In addition, the composition for improving the heart function of companion animals according to the present invention can be safely ingested without side effects even for long-term feeding, even for companion animals that are diseased or not healthy, and can stably supply balanced nutritional components with high convenience and palatability.

It will be fully understood that embodiments of the technical idea of the present invention may provide various effects not specifically mentioned.

1 is a view showing the results of Oil Red O staining of animal model heart tissue for administration of the composition according to Example.
Figure 2 is a graph showing the Oil Red O staining area of the heart tissue of the animal model for the administration of the composition according to the embodiment.
3 is a diagram showing the concentration of triglyceride in plasma of an animal model for administration of the composition according to Example.

Advantages and features of the present invention, and methods for achieving them, will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed subject matter may be thorough and complete, and that the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Hereinafter, the composition for improving heart function in companion animals according to the present invention will be described in detail with reference to preferred examples.

The composition for improving heart function in companion animals according to the present invention includes colostrum powder, L-carnitine and coenzyme Q10 as active ingredients.

In addition, the composition for improving heart function in companion animals according to the present invention may be included in a weight ratio of 2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, and 0.1 to 3 parts by weight of coenzyme Q10.

In addition, the composition for improving heart function in companion animals according to the present invention, in addition to the colostrum powder, L-carnitine and coenzyme Q10, meat protein, potato starch, tapioca starch, soybean powder, trehalose, fructooligosaccharide, vegetable glycerin, olive oil, taurine , chitosan, omega-3 fatty acids, L-arginine, vitamin C and vitamin E may be further included.

In addition, the composition for improving heart function in companion animals according to the present invention contains 2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, 0.1 to 3 parts by weight of coenzyme Q10, 25 to 35 parts by weight of meat protein, and potato starch. 12 to 18 parts by weight, 20 to 25 parts by weight of tapioca starch, 5 to 10 parts by weight of soybean powder, 2 to 6 parts by weight of trehalose, 3 to 7 parts by weight of fructooligosaccharide, 2 to 6 parts by weight of vegetable glycerin, 3 to 7 parts by weight of olive oil parts by weight, 0.1 to 1 parts by weight of taurine, 0.1 to 1 parts by weight of chitosan, 0.1 to 3 parts by weight of omega-3 fatty acids, 0.5 to 1.5 parts by weight of L-arginine, 0.1 to 1 parts by weight of vitamin C, and 0.1 to 1 parts by weight of vitamin E It may be included in a weight ratio of parts.

In addition, the present invention can manufacture a functional food comprising the composition for improving the heart function of companion animals. For example, as the functional food, various known types of food that can be supplied to companion animals such as nutritional supplements and feed additives can be applied to

The colostrum powder is prepared using colostrum, the colostrum refers to the milk that a mammal secretes within one week after delivery, and recently, the milk secreted within 48 to 72 hours after delivery is defined as colostrum.

In general, colostrum secreted within 48 hours contains a large amount of functional substances. Chemically, cow colostrum is a very complex liquid substance, rich in protein, carbohydrate, fat, vitamins, and minerals. It contains a large amount of immunomodulatory factors, physiologically active factors, antibacterial factors, growth hormones, growth factors, and cell division activators that are involved in the growth of organs and tissues and the development of the immune system.

Substances and factors with these immunological and physiological properties are mainly contained in colostrum whey, and a-lactalbumin and b-lactoglobulin account for 70-80%, and other protein components include serum albumin, lactoferrin, Immunoglobulin, growth factor, lysozyme, trace amount of bioactive factor, etc., many studies are being conducted to characterize each (Korean J. Food Sci. Technol. Vol.34, No.4, pp.694~699 (2002) )).

Studies have shown that atherosclerosis and cardiovascular disease are associated with altered immunity, damage to the heart and blood vessels produces antibodies that further impair the immune system, and in several studies, chlamydia-infectious pneumonia, etc., activate early-stage atherosclerotic lesions. and developmental causes. The immune factors of bovine colostrum help fight this bacteria, and in particular, the proline-rich-polypeptide contained in colostrum is expected to have a myocardial protective effect on patients diagnosed with cardiomyopathy, It suppresses cholesterol deposition in damaged blood vessels by regulating the immune response, and growth factors promote the repair and regeneration of cardiac cells, and the regeneration of new coronary vessels for collateral circulation.

In addition, in relation to blood pressure control function, the peptide present in colostrum lowers blood pressure by inhibiting angiotensin I-converting enzyme (ACE), an enzyme integrally involved in the renin-angiotensin system, thereby lowering blood pressure and reducing cardiac oxygen consumption. It has been reported as a result of research that it has antihypertensive and cardiovascular disease improvement effects by reducing

In a recent study in a rat model of myocardial infarction, after 28 days of ingestion of colostrum, the protective efficacy of cardiac tissue damage was observed through inhibition of lipid peroxidation (antioxidant) and reduction of cardiac damage markers (CKMB, LDH). It was confirmed that the efficacy was further enhanced when combined with therapeutic agents (ACE inhibitors, blood pressure regulator enalapril) (Journal of pharmacology and toxicology 9(1):37-45, 2014).

In addition, the colostrum contains Insulin-like Growth Factor-1 (IGF-1), which promotes overall growth and is known to have the ability to regulate cell growth and amplification.

In the present invention, as the colostrum powder, a known bovine colostrum extract powder may be used, or colostrum powder prepared by the following method may be used.

In order to prepare the colostrum powder, first, prepare bovine colostrum, mix the enzyme with the prepared bovine colostrum, and store it for enzymatic decomposition.

That is, in the step, after mixing the enzyme with the prepared bovine colostrum, the enzyme-mixed bovine colostrum can be enzymatically decomposed by storing it at a temperature of 36 to 38° C. for 20 to 30 hours.

In the above step, as the enzyme, a commercially available protease and xylanase may be used, for example, as the protease, chymotrypsin may be used and , The chymotrypsin is a digestive enzyme that causes protein degradation.

That is, the chymotrypsin is a digestive enzyme that causes protein degradation and breaks the peptide bond, and the chymotrypsin acts as a protease in the digestive system of mammals and other organisms. The chymotrypsin substrate includes tryptophan, tyrosine, phenylalanine, leucine, and methionine, and their carboxy terminus is cut off. Like other proteolytic enzymes, it triggers a reaction that breaks peptide bonds.

In addition, the xylanase (Xylanase) is an enzyme that hydrolyzes xylan into xylo-oligosaccharide or xylose, and is an enzyme used in bioenergy, food, fiber, paper and pulp industries. to be. Research is being actively conducted in the field of bioethanol production by decomposing and saccharifying vegetable fiber, the most abundant biological resource on the planet, and the production of high-quality paper and waste paper recycling, removal of turbidity in fruit drinks and beer, and the production of coffee, vegetable oil and starch. It is an enzyme that is already widely used industrially for purposes such as extraction and increasing the efficiency of feed use of unit animals.

Specifically, in the above step, the enzyme is mixed in a weight ratio of 0.01 to 0.03 parts by weight of a protease such as chymotrypsin and 0.008 to 0.012 parts by weight of xylanase with respect to 100 parts by weight of the prepared bovine colostrum. can be

Next, the enzyme-decomposed bovine colostrum may be mixed with a culture broth and a fermentation broth consisting of lactic acid bacteria.

In the above step, as the lactic acid bacteria, any one known lactic acid bacteria selected from the group consisting of Lactobacillales , Streptococcus thermophiles and Bifidobacterium longum may be used, and the The culture medium consists of peptone, malt extract, sodium citrate, potassium phosphate dibasic, glucose and distilled water, and the culture medium is 15 to 25 parts by weight of peptone, malt extract 5 to 15 parts by weight, 0.5 to 1.5 parts by weight of sodium citrate, 0.1 to 0.3 parts by weight of potassium dibasic phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water may be mixed in a weight ratio.

In the culture medium, the peptone is a nitrogen source that supplies proteins and supplies proteins necessary for the growth of microorganisms, and the malt extract contains various minerals, vitamin B group, and amino acids to help microorganisms grow, Silver is a mineral that maintains an optimized state for the growth of microorganisms by adjusting the pH of the culture medium, and can supply nutrients, and the glucose can function as an important component for lactic acid bacteria to metabolize sugar to produce organic acids.

Next, it is possible to prepare a fermented bovine colostrum by fermenting the bovine colostrum mixed with the fermentation broth.

In the above step, the fermentation may be performed by storing the bovine colostrum mixed with the fermentation broth at a temperature of 38 to 42° C. for 1 to 3 days.

Then, the fermented bovine colostrum is filtered to remove impurities, freeze-dried, and then pulverized to prepare colostrum powder.

In the above step, impurities remaining in the fermented bovine colostrum can be removed by filtering the fermented bovine colostrum using a known filter, and the filtered fermented bovine colostrum at a temperature in the range of -40°C to -30°C After freeze-drying for 1 to 3 hours, it can be pulverized to prepare colostrum powder.

In the present invention, by enzymatically decomposing bovine colostrum and fermenting lactic acid bacteria as described above to produce colostrum powder, the representative physiologically active material of colostrum, proline-rich-polypeptide (PRP), etc. effective dose can be increased.

The L-carnitine is an essential amino acid responsible for the production of energy in the heart. Since the heart obtains 60% of its required energy from fat, L-carnitine must be sufficiently present in order for cardiac muscle cells to function.

Carnitine deficiency has been reported in humans and dogs with dilated cardiomyopathy. According to a study published in the Journal of the American Heart Association, in a randomized study including a placebo control group, when L-carnitine 2g was consumed in heart failure patients, the survival rate after 3 years was significantly lower in the carnitine group. According to a report published in the <Journal of the International Heart Association>, L-carnitine was found to prevent damage to the heart by activating antioxidant and anti-inflammatory components.

The coenzyme Q10 is a substance similar to a vitamin found in mitochondria naturally produced in the body, and is defined as an essential cofactor involved in the energy production process of all cells.

Although the coenzyme Q10 is not a vitamin or mineral, it is called vitamin Q because it acts similar to a vitamin. After peaking at the age of 40, production drops by more than 30% and gradually decreases with age. In particular, the amount of coenzyme Q10 in the heart decreases the most.

When the coenzyme Q10 is deficient, there is a problem in the contraction and relaxation of the heart, and the high blood pressure state continues, causing heart disease. It is known that the production of coenzyme Q10 is reduced even when suffering from chronic diseases such as heart disease, Parkinson's disease, cancer, and diabetes.

Meanwhile, the organ in the body that needs the most energy is the heart, and the coenzyme Q10 strengthens the heart function through mitochondrial activation and helps the heart muscle move. Coenzyme Q10 may be useful for patients with angina pectoris, myocardial infarction, heart failure, or for humans and mammals with reduced heart function. Accordingly, Japan approved coenzyme Q10 as a prescription drug for congestive heart failure in 1974, and in the United States from the mid-1980s, the New York Heart Association concluded that taking coenzyme Q10 is a safe and effective long-term treatment for cardiomyopathy. It is widely known that it has an effect on heart health.

In addition, the coenzyme Q10 directly functions as a powerful antioxidant, and also helps the function of a powerful antioxidant in the body. For example, coenzyme Q10 is required for vitamin E antioxidant action. When coenzyme Q10 is deficient, vitamin E cannot inhibit the fat oxidation reaction and can rather accelerate it. Coenzyme Q10 acts to prevent this phenomenon of vitamin E.

In addition, coenzyme Q10 and L-carnitine have been reported to have beneficial functions on the heart function, and the combination of the two components is more effective with regard to heart function than using them individually.

According to the results of the study, the protective effect against ischemia and reperfusion of the rat model heart when combined ingestion was confirmed by confirming the lower purine secretion (a good index of myocardial energy balance) in the cardiac perfusate in the combined intake group of the two components. A synergistic effect was confirmed even higher, and when used in combination with other taurine, the improvement effect of cardiac function was further increased.

The coenzyme Q10 may be extracted from the heart of animals such as cattle, may be obtained by a synthetic method or a fermentation method, and is not particularly limited.

The meat protein may be included in the composition for improving the heart function of companion animals and act as a protein source. The meat protein may be duck meat, and in addition, duck meat and chicken meat may be mixed in a weight ratio of 4:1. may be

The duck meat is a good source of iron, phosphorus, and vitamin B compared to other meat, has a higher content of unsaturated fatty acids than beef or pork, and contains abundant essential amino acids such as lysine, valine, threonine, leucine, and methionine. It is nutritionally excellent and low in calories, so it has the advantage of being suitable as a diet for weight control, a diet for patients during convalescence, and a diet for the elderly with little physical activity.

In addition, while most other meats are acidic foods, duck meat is slightly alkaline suitable for the human body. Due to its strong sodium, calcium, lecidin, and serenium components, it reduces cholesterol in blood vessels and helps prevent stroke, arteriosclerosis, and high blood pressure. It is also known as a food that helps prevent osteoporosis, diabetes, neuralgia, and cancer cell development.

In addition, since duck meat oil is unsaturated fat, it contains linoleic acid and linoleic acid, which are fatty acids necessary for our body, to suppress the formation of cholesterol and facilitate blood circulation. It is significantly higher than beef, pork, and chicken, and is rich in essential amino acids and various vitamins. In particular, it has a high content of vitamin C, vitamin B1, and vitamin B2, which improves endurance, prevents loss of concentration, and eliminates chronic fatigue.

The chicken, unlike red meat or fish such as pork or beef, has a low fat content and calories, so it is an animal protein source suitable for people who need weight control, patients in recovery period, the elderly with little physical activity, and modern people who lack exercise. The calories of lean meat with the chicken skin removed are only 100~110kcal/100g, so it is a low-calorie, high-protein food than red meat, mackerel (183kcal/100g), or saury (165kcal/100g).

In addition, the chicken meat is characterized by thinner and softer muscle fibers than beef, and because fat is not mixed with muscle fibers like beef, it has a light taste and is easily digested and absorbed. It has a higher content than beef and is rich in nutrients such as calcium, phosphorus, thiamine, riboflavin, niacin, retinol and unsaturated fatty acids that are converted into vitamins in the body. It is known as an excellent nutritious food that has the effect of protecting the liver and alleviating muscle atrophy, fatigue, and deterioration of eyesight caused by liver dysfunction.

In addition, the chicken is largely divided into white meat and red meat depending on the part. The breast meat is white and low in fat, so it is light, and the leg meat part is red and has a unique flavor. do.

The potato starch may be included in the composition for improving the heart function of companion animals and function as a carbohydrate source. The potato starch has large starch particles, high purity, relatively long amylose and amylopectin chain lengths, the presence of phosphate ester groups in amylopectin, It has the ability to exchange specific cations that affect the viscous behavior, and the ability to form a thick, viscoelastic gel upon heating and cooling.

In addition, the potato starch has high viscosity, excellent film-forming ability, and low gelatinization temperature. Softness and elasticity are good when forming a gel, but the gel strength is weak. It tends to break more easily by heating and stirring.

In general, potatoes are a food resource that has been used by humans for a long time and contain a lot of starch, high-quality protein, vitamin C, vitamin B6, vitamin B8, potassium, phosphorus, magnesium, iron, dietary fiber, and phenol. It is a food with high nutritional value.

The tapioca starch is included in the composition for improving the heart function of companion animals and serves to form a carbohydrate source and viscosity. It contains ~30% of starch, and by crushing it, washing the starch with water to precipitate it, and drying it to make tapioca.

The potency of tapioca is close to zero sugar, fat and protein calories with 26 grams of carbohydrates per 100 calories. Additionally, tapioca is unsweetened and gluten-free, products made from cassava are easy to digest and are often recommended for people with digestive problems such as IBS or IBD, and can be consumed by people with sensitive digestive systems or people who have trouble eating wheat. have.

The soybean powder can be manufactured by pulverizing soybeans, which are responsible for supplementing and supplying protein and lipids lacking in rice, and are 35-40% protein, 15-20% oil It contains more than 80% of unsaturated fatty acids, which are effective in lowering cholesterol and preventing various cardiovascular diseases. The functional component of soybeans is structurally similar to the female hormone estrogen, and there are isoflavones that are effective in circulatory diseases such as high blood pressure and arteriosclerosis and osteoporosis. In addition, saponin is effective in anti-cancer and anti-aging, phytic acid easily binds to heavy metal ions harmful to the human body and is easily excreted from the body. In addition, trypsin inhibitor, hemaglutinin, oligosaccharide, etc. is known as

In addition, isoflavones (genistein, daidzein, glycitein), which are the most representative functional components of soybeans, have an antioxidant effect and are known to have an effect of inhibiting breast and ovarian cancer by blocking estrogen receptors.

As a functional ingredient other than the isoflavones, soybean peptide has activities such as cholesterol lowering, blood pressure lowering, immune revitalization, and platelet aggregation inhibition, and inositol and phytate are known to have anticancer effects. Inhibition, reduction of blood cholesterol, anticancer, HIV growth inhibition, anti-aging, etc. In addition, soybean component has been studied to be effective in skin cancer, bladder cancer, lung cancer, colon cancer, pancreatic cancer, esophageal cancer, prostate cancer, liver cancer, stomach cancer, etc. revealed through

The trehalose is a non-reducing disaccharide in which two glucose molecules form an alpha 1,1 bond, and is a substance present in many living things, such as plants, fungi, insects, and microorganisms. Trehalose is currently known to have various properties. It is used as an energy source within an organism or accumulated as a compatible solute when subjected to environmental stress, helping the individual to adapt to the environment. In particular, trehalose has the property of retaining moisture, so it is in the spotlight in a wide range of fields such as cosmetics, food and pharmaceuticals.

That is, the trehalose is a disaccharide in which two glucose is linked, and serves to protect the cell membrane or protein from being denatured in the cell. In particular, the effect of preventing denaturation that may occur during drying or freezing of the protein is excellent, and since it has a non-reducing property, there is an advantage in that the Maillard reaction does not occur.

The fructooligosaccharide refers to a mixture of ingredients in which 1 to 3 fructose are bonded to sugar using a fructose transferase using sugar as a substrate. It is known that the fructooligosaccharide has an effect of intestinal function that helps the growth of beneficial bacteria and suppresses harmful bacteria in the intestine, and helps smooth bowel movements.

The vegetable glycerin may be included in the composition for improving the heart function of companion animals and used as a binder for formulation stability.

The olive oil may be included in the composition for improving the heart function of companion animals to enhance palatability, and mono-unsaturated fatty acids, such as oleic acid, which play an important role in the formation of human cells and all organs in the body. acid) contains about 55 to 83% of the total fatty acid, and contains about 40 enzymes including vitamins A, B, C, D, E, F, K, etc. It is known to have an excellent effect in preventing diseases, diabetes, and obesity.

The taurine is an essential amino acid that performs a bioregulatory function, and the taurine is a component present in most living things except plants, and is widely present in all organs and tissues such as heart, lung, liver, brain, bone marrow, and maintains vital activity. It is one of the amino acids that play an essential role in our body, and it is an amino acid that can be synthesized in our body and usually participates in digestion and suppresses free radicals.

The taurine helps the action of enzymes to promote the secretion of bile acids in the liver, promotes the regeneration of liver cells, aids in alcohol decomposition, relieves the burden on the liver, improves liver function, prevents arteriosclerosis by reducing cholesterol, and prevents arteriosclerosis It works.

In addition, the taurine strengthens the contractility of the heart through the action of regulating the concentration of calcium ions, which play an important role in the contraction of the heart. In addition, the antioxidant function of taurine protects the heart muscle by preventing oxidative damage to the cell membrane of the heart muscle, lowering the excitability of the sympathetic nerve, preventing the stiffness of the arterial wall, and reducing fatigue.

The chitosan is an insoluble high molecular material in which an N-acetyl group is substituted with an amino group by deacetylating chitin, which is abundantly contained in crustaceans such as shrimp, crab, insect shells, and squid bones, and has a glucosamine bond, and its molecular structure is It is known as a very useful material for food and medicine because it has a structure very similar to that of human tissue, so it has excellent compatibility with the human body and does not cause immune rejection.

In addition, the chitosan is a cationic natural polymer that is abundantly present next to cellulose in nature, and the molecular structure of chitosan is very similar to that of cellulose, but an amino group (-NH ₂ ) exists in the molecule, so it has various physicochemical properties.

The main functions of chitosan include antibacterial, antifungal, heavy metal adsorption, physiological activity, human affinity, promotion of wound healing, cholesterol lowering, liver function improvement, blood sugar lowering action, etc. It is widely applied.

The omega-3 fatty acid (Omega-3 fatty acid) is distributed in the nerve cell membrane and the retina, and serves to transfer electrical stimulation from the cell membrane to the next cell at a high speed. It protects cells in the human body, maintains the structure of cells, and helps smooth metabolism. In addition, there is an effect of inhibiting the formation of a blood film, and promoting and strengthening the bone formation.

Examples of the omega-3 fatty acids include alpha-linolenic acid (LNA), docosahexaenoic acid known as DHA, eicosapentaenoic acid known as EPA, and stearidonic acid (SDA). , and ETA (eicosatetraenoic acid).

The recommended daily amount of the omega-3 fatty acids is 0.6 to 1 g, and it contains a lot of fish oil, plankton, seafood, soybean oil, breast milk, and the like. In particular, in the case of newborns and adolescents, a larger amount is needed to help normal tissue development. Deficiency is known to cause depression, schizophrenia, attention deficit/hyperactivity disorder, blurred vision, heart disease, etc., and may increase stress.

The docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are omega-3 fatty acids and are known as the most essential fatty acids among polyunsaturated fatty acids (PUFA). . These fatty acids are distributed in large amounts in animals such as blue-backed fish (mackerel, herring, salmon, tuna) and seal oil in nature, and are also present in small amounts in some plants. DHA is an essential component of nerve cells and is widely known to be important for maintaining brain and eye health. According to an epidemiological study of Greenlanders, EPA has a strong anti-cardiovascular disease function.

The omega-3 fatty acids are known to exhibit beneficial effects on arteriosclerosis by removing risk factors for circulatory disease or changing serum lipid composition or platelet aggregation function. In particular, DHA is tested as a component of retina and brain phospholipids It is said that it contributes to the improvement of brain functions, including the learning ability of animals.

The L-arginine exists as one of the amino acids constituting the protein, and although it is a non-essential amino acid for adults, it is an essential amino acid for infants. It belongs to the protein protamine present in sperm of fish, and in herring and salmon, about 70% of the constituent amino acids are arginine.

In addition, the L-arginine is also present in a free state in plant seeds. As a metabolic pathway in the living body, it is a component of the ornithine cycle discovered by HA Krebs et al., and is decomposed into urea and ornithine by the action of arginase, and is produced from citrulline and aspartic acid in the living body. L-Arginine protects the human body from the toxic effects of ammonia or large amounts of amino acids, and increases anti-inflammatory activity.

The vitamin C is included in the composition for improving the heart function of companion animals and can function as an antioxidant or antioxidant. The vitamin C (Vitamin C) is called ascorbic acid, and as a strong reducing agent, As one of the essential components in the body, scurvy occurs when the human body is deficient in vitamin C. Therefore, the human body cannot make its own vitamin C, so it must be consumed through vegetables or fruits.

In addition, the vitamin C is a water-soluble antioxidant that protects the body from free radicals and prevents cancer, arteriosclerosis, rheumatism, etc. protects In other words, as vitamin C prevents oxidation of cells, papers have been published that prevent cancer and various chronic diseases and inhibit aging.

The vitamin E serves to protect cells from free radicals, acts as an antioxidant along with beta-carotene and vitamin C, and can help blood circulation.

More specifically, the vitamin E is i) a physiological antioxidant, ii) involved in biofilm formation, iii) involved in prostaglandin synthesis, iv) inhibition of platelet coagulation, v) oxidation, reduction (electron transport) of the living body, and cell Regulate DNA biosynthesis, vi) disease suppression and immune function enhancement, vii) prevention and treatment of heavy metal poisoning with Se, viii) cell tissue protection with Se, xi) normal phosphorylation reaction (high-energy phosphate), ascorbic acid It is involved in synthesis, ubiquinone synthesis, sulfur-containing amino acid metabolism, etc. and x) vitamin B12 metabolism.

On the other hand, the composition for improving the heart function of companion animals according to the present invention is the colostrum extract (IGF-1, PRP), L-carnitine, coenzyme Q10, meat protein, potato starch, tapioca starch, soybean powder, trehalose, fructooligosaccharide, In addition to the composition consisting of vegetable glycerin, olive oil, taurine, chitosan, omega-3 fatty acids, L-arginine, vitamin C and vitamin E, green lipped mussel oil and liquid minerals may be further included, the colostrum extract (IGF-1, PRP) ), L-carnitine, coenzyme Q10, meat protein, potato starch, tapioca starch, soybean powder, trehalose, fructooligosaccharide, vegetable glycerin, olive oil, taurine, chitosan, omega-3 fatty acid, L-arginine, vitamin C and vitamin E Based on 100 parts by weight of the total composition consisting of, 0.1 to 1 parts by weight of the green lipped mussel oil and 0.5 to 1.5 parts by weight of the liquid mineral may be further included.

The green-lipped mussel oil is manufactured using green-lipped mussels (Perna Canaliculus), which is a kind of mussels that inhabit the coast of New Zealand and is called green-lipped mussel, and its scientific name is Perna. Canaliculus.

It has been reported that the green-lipped mussel contains omega unsaturated fatty acids related to antioxidant and anti-inflammatory properties, that is, a unique component that cannot be seen in mussels that are native to other regions, because the green-lipped mussel feeds mainly on plankton that has evolved to resist intense ultraviolet rays.

In addition, the unsaturated fatty acids found in the green lipped mussel have been known through clinical trials to have a 200-fold stronger anti-inflammatory effect than fish oil, linseed oil, evening primrose oil, and the like containing omega-3 fatty acids. Omega-3 fatty acids have been found to inhibit the occurrence of inflammatory diseases such as atherosclerosis and rheumatoid arthritis by interfering with the production of inflammatory mediators made from omega-6 fatty acids by competing with omega-6 linoleic acid. there is a bar

As the green-lipped mussel oil, green-lipped mussel oil prepared by the following method may be used.

First, the skin of the green-lipped mussel may be removed, and the green-lipped mussel from which the skin has been removed may be washed.

In the above step, the green-lipped mussel from which the skin has been removed can be washed with a sodium bicarbonate solution having a temperature of 10 to 20° C. and a concentration range of 1 to 3 (w/w)%, and the sodium bicarbonate (NaHCO3) is a food product. It is also used as an additive, is non-toxic, and may have functions such as penetration, diffusion, and expansion.

Next, the washed green-lipped mussels may be dried.

Drying of the washed green-lipped mussel may consist of a first drying and a second drying, wherein the first drying is performed by drying the washed green-lipped mussel at a temperature of 70 to 80° C. for 3 to 5 hours. Residual moisture in green-lipped mussels can be removed primarily.

In addition, in the second drying, the first dried green-lipped mussel may be dried with hot air, and the second drying is performed by applying hot air to the first dried green-lipped mussel at a temperature of 110 to 130° C. for 1 to 3 hours. This can be done by adding

Next, the second dried green-lipped mussel can be pulverized and then aged to prepare an aged green-lipped mussel powder.

In the above step, the grinding of the second dried green-lipped mussel is pulverized by pulverizing the second dried green-lipped mussel to a particle size of 5 to 10 mm, and the aging of the powdered green-lipped mussel is the powdered green mussel. It can be carried out by storing the lipped mussels at a temperature of 5 to 10° C. for 3 to 7 hours.

Then, the green-lipped mussel aged powder can be enzymatically decomposed by mixing the enzyme and acid and storing the mixture.

That is, in the above step, the enzyme and acid are mixed in the aged powder of green lipped mussel, and then the aged powder of green lipped mussel in which the enzyme and acid are mixed is stored at a temperature of 36 to 38° C. for 20 to 30 hours, thereby enzymatic decomposition. .

In the above step, known commercially available protease (Protease), chitinase (chitinase), pectinase (Pectinase) and xylanase (Xylanase) are used as the enzyme, and citric acid and ascortic acid are used as the acid. Binic acid may be used, specifically, 0.01 to 0.03 parts by weight of the protease, 0.03 to 0.05 parts by weight of the chitinase, and pectinase based on 100 parts by weight of the whole aged powder of green lipped mussel. 0.005 to 0.009 parts by weight, 0.008 to 0.012 parts by weight of Xylanase, 0.01 to 0.1 parts by weight of citric acid, and 0.01 to 0.1 parts by weight of ascorbic acid may be mixed in a weight ratio.

Next, the fermented green-lipped mussel can be prepared by mixing the lactic acid bacteria culture solution with the enzyme-decomposed green-lipped mussel aged powder and fermenting it.

In the above step, after mixing in a weight ratio of 1 to 3 parts by weight of the lactic acid bacteria culture solution to 100 parts by weight of the enzymatically decomposed green-lipped mussel aged powder, fermentation may be performed at a temperature of 40 to 42° C. for 20 to 30 hours.

In addition, in the above step, the lactic acid bacteria culture medium was adjusted to pH 2.8 to 3.2 using 1.2N HCl, and then the lactic acid strain was separated from kimchi, and the separated lactic acid strain was used in MRS broth (Oxoid, England). After culturing at 37-39°C for 20-25 hours, dilute to 1×1.0 ^8-5 ×1.0 ⁸ CFU/mL, and then centrifuge at 10,000-15,000 rpm for 10-15 minutes to separate only the supernatant And, after filtering the supernatant (Supernatant) with a 0.45㎛ syringe filter (syringe filter), mixed in a weight ratio of 1,000 to 2,000 parts by weight of sterilized distilled water with respect to 100 parts by weight of the filtered supernatant It is prepared by diluting, the lactic acid bacteria As a lactic acid strain isolated from fermented food, kimchi, specifically, the lactic acid strain includes Lactobacillus curvatus , Weissella viridescens , Lactobacillus plantarum ) and Any one or more known lactic acid strains selected from the group consisting of Leuconostoc lactis may be used.

Next, the green-lipped mussel oil can be prepared by separating the oil using the fermented green-lipped mussel.

The configuration, such as separating the oil using the green-lipped mussel fermented product in the above step, is a known technique to those of ordinary skill in the art, and for convenience of explanation and clarity of the technical idea of the present invention, A detailed description thereof will be omitted.

As the liquid mineral, a liquid mineral prepared by the following method may be used, and the liquid mineral may supply various minerals to companion animals. That is, in the present invention, the liquid mineral may be prepared from seawater, and as the liquid mineral, a liquid mineral from which salt has been sufficiently removed may be used.

In order to prepare the liquid mineral, first, seawater may be collected.

In the Sanji stage, deep ocean water can be used as the seawater, and the deep ocean water is usually 200m or less seawater is called deep ocean water, and unlike surface seawater, sunlight does not reach it, so plankton And because living things do not proliferate, the concentration of nutrients is high and there is no contaminant present in the surface seawater because it does not mix with the surface seawater due to the density difference according to the water temperature. However, cleanliness with very little organic matter, eutrophication rich in inorganic nutrients that are very important for plant growth, and the mineral balance characteristic of a balanced existence of various mineral components, and the cluster of water molecules for a long time at high pressure and low temperature. ) is subgrouped, so it has low surface tension and aging characteristics such as aging with water with good permeability.

In particular, salt containing a large amount of mineral components produced in deep sea water contains various mineral components such as calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn) necessary for the growth of basophilic fermenting microorganisms. It contains high concentrations of nutrients, such as nitrates, phosphates, and silicates, because living things do not live at low temperatures without direct sunlight. There is this.

Next, the first seawater may be prepared by first drying the collected seawater, and separating and removing foreign substances or solids precipitated after the first drying using a filter.

The first drying of the seawater may be performed until 1/5 to 2/5 of the mass of the first collected seawater is evaporated by naturally drying the collected seawater at a temperature of 20 to 40°C for 10 to 20 days. , The first seawater can be obtained by removing the precipitated foreign substances or salt crystals generated after the first drying and collecting only liquid seawater.

Next, the second drying is performed after mixing the activated carbon and pure jet powder with the first seawater, and the second drying is performed by separating and removing foreign substances or solids and activated carbon and pure jet powder precipitated after the second drying using a filter. seawater can be produced.

In the second drying of the first seawater, the activated carbon may implement the effect of adsorption and removal of harmful substances, and the Sungit powder may perform antioxidant, sterilization, stabilization and decomposition functions of pollutants, etc., and the first seawater The second drying is carried out by mixing 1 to 3 parts by weight of activated carbon and 0.1 to 0.5 parts by weight of pure git powder to 100 parts by weight of the first seawater, and then drying at a temperature of 20 to 40° C. for 1 to 5 days. can

In the second drying of the first seawater, the activated carbon is a carbon aggregate with micropores developed, and the internal surface area can be changed by the activation process, and the absorption and absorption properties in which it is adsorbed with the surrounding liquid or gas by the carbon atom functional group inside It has excellent adsorption properties.

The activated carbon can implement the effect of adsorption and removal of harmful substances, the activated carbon has a hardness of 93 mass fraction % or more, a packing density of 0.51 to 0.53 g/ml, a specific surface area of 800 to 900 m ² /g, and the pore distribution has a pore diameter of 4 Å to 7Å, iodine adsorption power of 1,100 mg/g or more, pore volume of 0.51 ml/g, pH 7-7.8, phenol adsorption power of 17.5 ml/g, M·B discoloration power of 153 ml/g or more may be used.

The pure jit powder may be produced by pulverizing pure jit minerals, and the shungite is a material containing SiO ₂ (silicate) and C ₆₀ (fullerene) as main components, and a material containing natural fullerene. means Pure shungite containing more than 90% fullerene is called elite shungite or noble shungite, and pure shungite containing less than 60% fullerene is called normal shungite.

The representative functions of the Sunjit include an antioxidant function, an electromagnetic wave blocking function, a purification and decomposition function of contaminants, and a sterilization and antibacterial function. As a powerful natural antioxidant, Sunjit has the function of increasing human immunity against numerous diseases, purifying water and air by adsorbing pollutants, and has the ability to self-decompose adsorbed pollutants.

In addition, since the Sunjit has antibacterial and sterilizing properties, it has a function of removing more than 99% of only E. coli, Pseudomonas aeruginosa, Staphylococcus aureus and harmful bacteria, and has a function of blocking harmful electromagnetic waves and emitting a large amount of far infrared rays.

The pure jit powder may be used pure jit powder prepared by the following method.

First, the pure jite mineral may be prepared and washed to remove foreign substances attached to the pure jite mineral.

The cleaning of the pure jite mineral may be performed by washing the surface of the pure jite mineral using purified water, thereby removing foreign substances remaining on the surface of the pure jite mineral.

Next, the washed pure jit mineral may be first pulverized to prepare a pulverized pure jit granulated product.

In the above step, by first pulverizing the washed pure jite mineral using a granulator, impurities or organic residues of the pure jite mineral can be removed in a later process, and the first pulverization is performed using a granulator. , may be performed using a known granulator such as a drum granulator or a compression pelletizer, but is not necessarily limited to the granulator described above.

Next, the powdered raw material may be immersed in the mixed solution.

In the above step, impurities or organic residues contained in the coarsely granulated and pulverized pure jite can be removed by immersing the pulverized pure jite in the mixed solution. to 1.0 wt%, persulfate 0.01 to 0.05 wt%, acetic acid 1 to 3 wt%, and the remaining amount of purified water, and may be performed by immersing the milled Sunjit granulated powder in the mixed solution for 20 to 40 minutes.

In addition, as the phosphate compound in the above step, sodium phosphate monobasic (NaH ₂ PO ₄ ), sodium phosphate dibasic (Na ₂ HPO ₄ ), sodium phosphate tritertiary (Na ₃ PO ₄ ), potassium phosphate first (KH ₂ ) PO ₄ ), potassium phosphate dibasic (K ₂ HPO ₄ ), ammonium phosphate monobasic ((NH ₄ )H ₂ PO ₄ ), ammonium phosphate dibasic ((NH ₄ ) ₂ HPO ₄ ), and ammonium tertiary phosphate (( Any one or more selected from the group consisting of NH ₄ ) ₃ PO ₄ ) may be used, and as the persulfate, ammonium persulfate ((NH ₄ ) ₂ S ₂ O ₈ ), sodium persulfate (Na ₂ S ₂ O ₈ ) ) or potassium persulfate (K ₂ O ₈ S ₂ ) Any one or more selected from may be used.

Then, after separating the granulated and pulverized raw material from the mixed solution, the granulated and pulverized raw material may be heated and calcined using a kiln.

In the above step, it is possible to remove and activate the contaminants adsorbed on the micropores of the granulated pure Jit by heating the separated pure Jit granulated and pulverized product using a kiln. After being put into the kiln, it can be carried out by heating at a temperature of 1,100 to 1,300 ℃ for 50 to 100 minutes.

Next, a second pulverization of the calcined pulverized pure jit may be performed to prepare a pulverized pulverized pure jite.

In the above step, it may be carried out by pulverizing the calcined pure jit granulated product using a ball mill or various known pulverizers. can

Next, impurities may be removed from the pulverized material of pure Jit and sterilized to prepare a pure Jit powder.

In the above step, impurities such as heavy metals that may remain in the pulverized pure jit can be removed by rotating the pulverized pure jit in a rotary tub equipped with a magnet. It is possible to remove impurities such as heavy metals that may be included in the Sunjit fine pulverized product by rotating it for 10 to 20 minutes at a rotation speed of 500 to 1000 RPM after being put into the installed rotary tube.

Subsequently, the third seawater can be prepared by mixing the microporous body with the second seawater, evaporating the moisture by first heating, and separating and collecting only the liquid using a filter.

In the above step, calcium sulfate (CaSO ₄ ) and sodium chloride (NaCl) components contained in the second seawater may be precipitated first by mixing the microporous body with the second seawater and then heating the second seawater. It is possible to prevent the seawater from being rapidly heated and to allow the produced calcium sulfate (CaSO ₄ ) and sodium chloride (NaCl) components to be easily collected on the surface of the microporous body.

In the above step, after mixing in a weight ratio of 1 to 5 parts by weight of the microporous body with respect to 100 parts by weight of the second seawater, it is heated at a temperature of 80 to 85° C. for 5 to 10 hours to evaporate moisture, thereby being contained in the second seawater. Calcium sulfate (CaSO ₄ ) and sodium chloride (NaCl) components are precipitated, and the calcium sulfate (CaSO ₄ ) and sodium chloride (NaCl) components are precipitated and the remaining seawater is separated to prepare a third seawater in which mineral components are concentrated can do.

As the microporous body, a microporous body prepared by the following method may be used.

First, to prepare the microporous body, unburned carbon powder, fly ash, loess and dredged soil are prepared, and water can be prepared as a binder for molding the unburned carbon powder, fly ash, loess and dredged soil into a certain shape. At this time, the loess and dredged soil may be used to improve the caking properties of unburned carbon powder and fly ash.

In addition, the unburned carbon powder, fly ash, loess and dredged soil are each mixed in a weight ratio of 10 to 15 parts by weight of unburned carbon powder, 8 to 12 parts by weight of fly ash, 2 to 4 parts by weight of loess and 3 to 7 parts by weight of dredged soil, Water may be mixed in a weight ratio of 30 to 40 parts by weight based on 100 parts by weight of the total unburned carbon powder, fly ash, loess and dredged soil.

Next, after preparing a mixture by mixing the unburned carbon powder, fly ash, loess, dredged earth and water, the mixture may be molded into a predetermined shape to form a molded body.

That is, the molded body is mixed in a weight ratio of 10 to 15 parts by weight of unburned carbon powder, 8 to 12 parts by weight of fly ash, 2 to 4 parts by weight of loess and 3 to 7 parts by weight of dredged soil, and the unburned carbon powder, fly ash, loess and dredged soil. After preparing a mixture by mixing in a weight ratio of 30 to 40 parts by weight of water with respect to 100 parts by weight of the total, a molded body can be manufactured by molding the mixture into various shapes such as spherical shape and pellet shape.

Next, the molded body may be heated and fired.

The firing of the molded body may be fired by heating the molded body in a kiln, for example, a tunnel kiln or a discontinuous kiln may be used as the kiln, and the kiln has an internal temperature of 400 to 450° C. It can be maintained at a temperature of, and then fired in an oxidizing or reducing (neutral) atmosphere for 3 to 5 hours while gradually raising the temperature to 1200 to 1300 °C after putting the molded body inside the kiln.

Then, the calcined molded body may be cooled to prepare a microporous body.

Cooling of the fired molded body in the above step can be cooled for 1 to 3 hours at a temperature of 200 to 250 ℃ after taking out the molded body from the kiln, and then cooled at a temperature of 30 to 50 ℃ for 10 to 12 hours, By cooling the fired molded body through the two-step cooling process as described above, it is possible to prevent surface cracking and a decrease in compressive strength of the fired molded body due to rapid cooling, and also, shrinkage can be prevented.

Next, a liquid mineral may be prepared by cooling and crystallizing the third seawater and then removing the solid content.

In the above step, after introducing the third seawater into the cooling device, the temperature of the cooling device is lowered to form a crystallized solid by lowering the temperature of the third seawater, and the solid is removed using a filter, etc. Minerals can be produced.

That is, in the above step, as the temperature of the cooling device is lowered, solids containing salt contained in the third seawater accommodated in the cooling device may be precipitated. A liquid mineral can be prepared by maintaining it and cooling it for 20 to 30 hours to crystallize it and then removing the solid content.

Hereinafter, examples of the composition for improving heart function in companion animals according to the present invention will be described in more detail.

<Example>

Colostrum powder 4 parts by weight, L-carnitine 5 parts by weight, coenzyme Q10 2 parts by weight, meat protein 30 parts by weight, potato starch 16 parts by weight, tapioca starch 23 parts by weight, soybean powder 8 parts by weight, trehalose 4 parts by weight, fructo Oligosaccharide 5 parts by weight, vegetable glycerin 4 parts by weight, olive oil 5 parts by weight, taurine 0.5 parts by weight, chitosan 0.5 parts by weight, omega-3 fatty acid 2 parts by weight, L-arginine 1 part by weight, vitamin C 0.5 parts by weight and vitamin E 0.5 A composition for improving the heart function of companion animals was prepared by mixing in a weight ratio of parts by weight.

Experimental Example 1: Experimental animal design and experimental diet composition

As the experimental animals, 8-week-old C57BL/6 male mice (Jackson Laboratory) were bred under constant conditions (temperature: 22±2° C., relative humidity: 55±10%, circadian cycle: 12 hours). Five animals were grouped, and water and food were freely supplied in a cage, and they were acclimatized for one week before the experiment and used in the experiment.

After the acclimatization period was over, the composition prepared in the above example was divided into 5 groups and the diet was fed for 12 weeks as shown in [Table 1] below.

army food type Composition (mg/kg) Experimental Example 1 LFDs - Experimental Example 2 HFD - Experimental Example 3 HFD 3.5 Experimental Example 4 HFD 6 Experimental Example 5 HFD 12

LFD (10% kcal as fat; D12450B, Research Diets Inc)

HFD (60% kcal as fat; D12492, Research Diets Inc)

Experimental Example 2: Heart function test (blood pressure, heart rate, echocardiography, etc.)

The effect of feeding the composition on the weakened heart function in an animal model was investigated. Specifically, the experimental animal was fixed in a fixation device, occlusion and volume-pressure recording (VPR) were placed on the tail, and CODA6™ non-invasive BP monitoring system (Kent Scienrific, Torrington, CT) was installed. Blood pressure was measured using Tailcuff plethysmography. After 3 days of blood pressure measurement training, systolic, average, diastolic blood pressure and heart rate were measured.

In addition, the experimental animals were anesthetized with Avertine, and transthoracic ultrasound examination was performed using a High-resolution Imaging System (Vevo 770, VisualSonics, Canada) equipped with a High-frequent Ultrasound Prove (RMV-707B).

Heart rate was measured during anesthesia, and ejection fraction (EF), fractional shortening (FS), and left ventricular (LV) mass were calculated using Vevo770 software. The final result is expressed as the average value of 10 cardiac cycles.

division Experimental Example 1 Experimental Example 2 Experimental Example 3 Experimental Example 4 Experimental Example 5 LVID;d(mm) 33.1±0.18 3.621±0.24 3.57±0.18 3.54±0.22 3.38±0.23 LVID;s(mm) 1.37±0.24 1.72±0.38 1.70±0.38 1.69±0.25 1.52±0.18 LV vol;d (μl) 43.12±5.45 55.51±9.28 55.14±5.81 53.74±8.17 49.85±8.86 LV vol;s (μl) 4.88±2.14 9.45±4.71 9.18±4.77 7.84±4.15 6.57±2.31 %EF 88.86±3.34 82.60±10.15 83.65±8.68 85.11±4.48 86.47±3.25 %FS 58.34±4.59 47.27±11.15 50.17±10.12 51.81±4.75 57.02±4.15 LV mass (mg) 79.13±3.35 97.15±15.46 95.84±13.33 90.64±18.26 82.94±18.14 HR (beats/min) 697.73±53.35 677.77±50.58 689.18±34.90 675.86±36.11 667.45±27.75 Systolic BP (mmHg) 110.82±5.33 113.16±3.82 97.80±11.44 98.91±10.41 109.40±5.57 Diastolic BP (mmHg) 76.75±6.86 77.70±5.65 69.75±10.10 70.68±10.27 79.34±4.75 Mean BP (mmHg) 89.78±6.44 88.94±3.75 78.68±10.47 79.78±10.22 89.12±4.87

LVID;d (left ventricular end diastolic diameter)

LVID;s (left ventricular end systolic diameter)

LV vol;d (left ventricular end diastolic volume)

LV vol;s (left ventricular end systolic volume)

Ejection fraction (EF)

FS (fractional shortening)

LV mass (left ventricular mass)

HR (heart rate)

Blood pressure (BP)

Referring to [Table 2], as a result of measuring cardiac function-related factors, the HFD diet group (Experimental Example 2) had left ventricular end diastolic diameter (LVID; d), left ventricular end systolic diameter compared to the control group (Experimental Example 1). (LVID;s), left ventricular end diastolic capacity (LV vol;d), and left ventricular end systolic capacity (LV vol;s) were increased. This means left ventricular hypertrophy. In addition, ejection fraction (EF) and compartment shortening rate (FS), which are indicators of ventricular contractile function, were decreased compared to the control group (Experimental Example 1). Therefore, it was confirmed that cardiac hypertrophy caused by HFD diet causes cardiac dysfunction.

On the other hand, left ventricular hypertrophy and weakening of ventricular systolic function due to the HFD diet were suppressed in the 12 mg/ml administration group of the composition according to the example (Experimental Example 5). Therefore, it can be seen that the composition of the present invention is effective in preventing and treating heart damage by improving the weakened heart function in an obese animal model.

Experimental Example 3: Measurement of cardiac lipid accumulation

Chronic obesity is long-term associated with specific lipid metabolism disorders. Therefore, the effect of administration of the composition according to the above example on the accumulation of cardiac lipids induced in an obese animal model was investigated using Oil Red O staining and plasma triglyceride quantification.

Specifically, for Oil Red O staining, cryosections obtained from cardiac tissue samples from experimental animals are fixed with 10% buffered formalin solution for 5 minutes, and then rinsed with water. After soaking in isopropanol for 30 seconds, the Oil Red O solution is treated at room temperature for 1 hour. Rinse twice with 60% isopropanol and counterstain with hematoxylin for 30 seconds. After rinsing with water to remove hematoxylin, the stained area was confirmed using a microscope.

The results are shown in FIGS. 1 and 2 and [Table 3] below.

In addition, the plasma triglyceride concentration was measured using a triglyceride colorimetric assay kit (Cayman Chemical, CA). The results are shown in FIG. 3 and [Table 4] below.

division Oil Red O dyeing area Experimental Example 1 1.3 Experimental Example 2 9.6 Experimental Example 3 7.5 Experimental Example 4 4.8 Experimental Example 5 4.3

division Plasma triglyceride concentration (mg/dL) Experimental Example 1 60.5 Experimental Example 2 119.5 Experimental Example 3 90.2 Experimental Example 4 101.5 Experimental Example 5 62.5

Referring to [Table 3] and [Table 4], the plasma triglyceride concentration of the HFD diet group (Experimental Example 2) was significantly increased compared to the control group (Experimental Example 1). On the other hand, the plasma triglyceride concentration was significantly decreased in the 12 mg/ml administration group (Experimental Example 5) among the composition administration groups.

Correspondingly, in the Oil Red O staining results, the dyeing area of the HFD-fed group (Experimental Example 2) was significantly increased compared to the control group (Experimental Example 1), but the dyeing area of the composition-administered group (Experimental Examples 3 to 5) was decreased. This shows that the composition has a function of regulating cardiac lipid metabolism in obesity induced by HFD diet.

Therefore, it can be seen that the composition according to the embodiment is effective in preventing and treating heart damage due to obesity by inhibiting increased cardiac lipid accumulation due to lipid metabolism disorders in an obese animal model.

Experimental Example 4: Inhibition of cardiac apoptosis (TUNEL staining)

It has been reported that cardiac lipid accumulation and oxidative stress are major factors in cardiac dysfunction and cardiac apoptosis in rodents and humans. Therefore, the effect of administration of the composition according to the embodiment on cardiac apoptosis with inhibition of lipid accumulation and antioxidant effect was investigated.

Specifically, the heart tissue sections of experimental animals were fixed with 10% neutral buffered formalin, penetrated with paraffin, and sliced to a thickness of 4 μm. Cardiac cell death was confirmed through terminal deoxynuclotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining of the paraffin-free tissue section using the ApopTag In Situ kit (Chemicon, Temecula, CA), and the results are shown in Table 5 below. ] is shown.

division Number of TUNEL staining positive cells Experimental Example 1 3 Experimental Example 2 14 Experimental Example 3 9 Experimental Example 4 8 Experimental Example 5 7

Referring to [Table 5], as a result of confirming cardiac apoptosis through TUNEL staining, the HFD diet group (Experimental Example 2) significantly increased cardiac apoptosis, whereas the composition administration group (Experimental Examples 3 to 5) had increased cardiac apoptosis was significantly inhibited. Therefore, it was confirmed that the composition according to the example was effective in preventing and treating heart damage by inhibiting cardiac apoptosis through inhibition of lipid accumulation and antioxidant efficacy.

As described above, a preferred embodiment of the present invention has been described, but those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential features thereof. will be able Therefore, it should be understood that the embodiment described above is illustrative in all respects and not restrictive.

Claims (6)

A composition for improving heart function in companion animals, comprising colostrum powder, L-carnitine and coenzyme Q10 as active ingredients.
The method of claim 1,
The composition for improving heart function in companion animals, characterized in that it contains 2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, and 0.1 to 3 parts by weight of coenzyme Q10.
3. The method of claim 2,
In addition to the colostrum powder, L-carnitine and coenzyme Q10, meat protein, potato starch, tapioca starch, soybean powder, trehalose, fructooligosaccharide, vegetable glycerin, olive oil, taurine, chitosan, omega-3 fatty acid, L-arginine, vitamin C And a composition for improving the heart function of companion animals, characterized in that it further comprises vitamin E.
4. The method of claim 3,
2 to 6 parts by weight of the colostrum powder, 3 to 7 parts by weight of L-carnitine, 0.1 to 3 parts by weight of coenzyme Q10, 25 to 35 parts by weight of meat protein, 12 to 18 parts by weight of potato starch, 20 to 25 parts by weight of tapioca starch, Soybean powder 5 to 10 parts by weight, trehalose 2 to 6 parts by weight, fructooligosaccharide 3 to 7 parts by weight, vegetable glycerin 2 to 6 parts by weight, olive oil 3 to 7 parts by weight, taurine 0.1 to 1 parts by weight, chitosan 0.1 to 1 Companion animal heart function, characterized in that it contains 0.1 to 3 parts by weight of omega-3 fatty acids, 0.5 to 1.5 parts by weight of L-arginine, 0.1 to 1 parts by weight of vitamin C, and 0.1 to 1 parts by weight of vitamin E improvement composition.
5. The method of claim 4,
The composition for improving the heart function of companion animals, characterized in that the colostrum powder uses bovine colostrum extract powder, and duck meat is used as the meat protein.
A functional food comprising any one composition selected from claims 1 to 5.

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