KR20220056493A - Manufacturing method for Opuntia Humifusa extract and a cosmetic composition comprising Opuntia Humifusa extract prepared thereby - Google Patents

Abstract

The present invention relates to a method for manufacturing an Opuntia humifusa extract and a cosmetic composition comprising the Opuntia humifusa extract manufactured thereby, and more particularly, relates to a method for manufacturing an Opuntia humifusa extract which can reduce the viscosity of the Opuntia humifusa extract by extracting through low temperature concentration under reduced pressure after enzyme treatment, and a cosmetic composition comprising the Opuntia humifusa extract manufactured by the manufacturing method which shows excellent moisturizing ability as well as low discomfort when used by lowering the viscosity.

Description

TECHNICAL FIELD [0002] A method for manufacturing a chrysanthemum extract and a cosmetic composition comprising an extract prepared thereby

The present invention relates to a method for preparing a chrysanthemum extract and a cosmetic composition comprising the extract prepared thereby, and more particularly, to extract by concentration under reduced pressure at a low temperature after enzyme treatment, thereby reducing the viscosity of the cheonnyeoncho extract. It relates to a cosmetic composition comprising an extract of chrysanthemum prepared by the above preparation method, which exhibits excellent moisturizing power as well as a low discomfort during use by lowering the method and viscosity.

The importance of moisturizing the skin is growing as the wearing of a mask is made compulsory due to the recent Corona 19, and as more indoor activities increase, skin irritation due to the mask increases, and the skin exposure time to a dry environment is prolonged.

In the case of glycerin and hyaluronic acid, which are commonly used in the present cosmetic composition, when the content is increased to increase the moisturizing effect, the viscosity of the cosmetic increases and the feeling of use deteriorates. In addition, when the content of glycerin is increased, there is a problem that glycerin may rather take away moisture from the skin and make it dry, and people with allergies may cause itching of the skin.

On the other hand, as consumers' anxiety about the harmfulness of chemicals in daily life products is increasing, the tendency to prefer natural products is increasing. In line with this trend, development of cosmetics using natural products with various functions Many studies have been reported on the development of and effective ingredients.

Opuntia humifusa is a cactus that can overwinter inland among the cacti of the genus Opintia that grows wild in Korea. Since the shape of the stem is similar to that of the palm, it is also called a palm cactus, and it is a fleshy perennial grass, that is, a perennial flowering plant. There are many shapes, such as a shape, a ball shape, and an oval shape, and they are classified according to the shape.

It is known that by reducing its own moisture by less than half, it can overwinter without freezing to death even at -20°C, so it can maintain vitality. It is not limited, and as all parts from the roots are used as raw materials for medicines, health functional foods, and cosmetics, safety is guaranteed, and it is expected as a high-value crop that can raise high income.

Cheonnyeoncho contains a large amount of nutrients such as dietary fiber, minerals, amino acids, mucopolysaccharides, and antioxidants taxifolin, polyphenols, vitamins, and flavonoids. It is known to have excellent antioxidant, anti-allergic, anti-cancer, and antibacterial effects, and is known to have an excellent moisturizing function when used as a cosmetic product with more than 80% moisture.

However, in the case of the extract of chrysanthemum extract extracted by the conventional method, the viscosity is very high due to pectin, the main component, and thus there is a problem in that the moisturizing power is rather reduced when a cosmetic is manufactured using it.

Korean Patent Registration No. 10-1959225 Korean Patent No. 10-1946146 Korean Patent Registration No. 10-1731480

It is an object of the present invention to provide a manufacturing method capable of reducing the viscosity of the chrysanthemum extract by extracting the cheonnyeoncho by enzymatic treatment with pectin-degrading enzyme and then concentrated under reduced pressure at low temperature, to improve the above problems, It is to provide a cosmetic composition comprising a chrysanthemum extract, which can provide a refreshing feeling of use due to low stickiness and low viscosity even when the extract prepared by the present invention is used in excess, and can also provide excellent moisturizing and absorption.

In order to achieve the above object, the present invention

Washing the cheonnyeoncho (Opuntia humifusa) (S10);

Drying the millennium plant washed with water in the step S10 at 30 to 60° C. for 15 hours to 30 hours (S20);

Extracting the cheonnyeoncho extract using a solvent of 30 to 90% concentration of the dried cheonnyeoncho in step S20 (S30);

The step of reacting the extract of cheonnyeoncho in step S30 with a pectin-degrading enzyme at 15 to 35° C. for 30 minutes to 2 hours (S40);

Enzyme inactivation treatment step of 20 to 30 ℃, 1 hour to 2 hours of the extract of chrysanthemum reacted with the pectin-degrading enzyme of step S40 (S50);

Centrifuging the inactivated chrysanthemum extract of step S50 to prepare an enzyme-treated chrysanthemum extract (S60); and

It provides a method for producing a cheonnyeoncho extract comprising a step (S70) of preparing a cheonnyeoncho extract by concentrating the enzyme-treated cheonnyeoncho extract of the step S60 under reduced pressure.

The cheonnyeoncho (Opuntia humifusa) may use any one or more selected from the group consisting of stems, flowers, seeds, fruits and roots.

The solvent of step S30 may be any one or more selected from the group consisting of hexane, ethanol, methanol, distilled water, propanol, ammonia, ethylene, aqueous alcohol solution, ethyl acetate, butylene glycol, propylene glycol and dichloromethane.

The pectinase in step S40 is pectinase, pectinlyase, polygalacturonase, cellulase, beta-glucanase, xylanase, arabanase, beta-glucanase, amylase and a group consisting of hemicellulase It is preferable that any one or more selected from

It is preferable that the amount of the pectin-degrading enzyme of step S40 is 5 to 30 parts by weight based on 100 parts by weight of the extract of cheonnyeoncho in step S30.

It is preferable that the conditions of the low-temperature vacuum concentration in step S50 are a temperature of 30 to 80° C., a pressure of less than 1 atm, and 3 to 24 hours.

In addition, it provides a cheonnyeoncho extract prepared by the manufacturing method of the present invention.

In addition, there is provided a cosmetic composition comprising the extract of cheonnyeoncho produced by the manufacturing method of the present invention.

According to the method for producing a chrysanthemum extract according to the present invention, by using a process of pectin-degrading enzyme treatment and low-temperature and reduced-pressure concentration, the active ingredient contained in cheonnyeoncho is effectively extracted, and the binding of pectin included in chrysanthemum is reduced to reduce the viscosity As a result, even if the content of the extract of chrysanthemum is increased in the cosmetic composition, the stickiness and viscosity are low, giving a fresh feeling of use, thereby reducing the discomfort of consumers, and can be used as cosmetics in various formulations.

In addition, by using a natural material, chrysanthemum, the chemical composition is minimized, so it is eco-friendly and can reduce the side effects of the human body.

1 is a flowchart of a method for producing a cheonnyeoncho extract according to the present invention.

Hereinafter, the present invention will be described in detail. In describing the present invention, detailed descriptions of related known configurations or functions may be omitted.

The terms or words used in the present specification and claims are not limited to a conventional or dictionary meaning, but should be interpreted as meanings and concepts consistent with the technical matters of the present invention.

The embodiments described in this specification and the configurations shown in the drawings are preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention, so various equivalents and modifications that can replace them at the time of the present application there may be

The present invention relates to a method for preparing a cheonnyeoncho extract and a cosmetic composition comprising the cheonnyeoncho extract prepared thereby.

The method for producing a cheonnyeoncho extract according to the present invention is

Washing the cheonnyeoncho (Opuntia humifusa) (S10);

Drying the millennium plant washed with water in the step S10 at 30 to 60° C. for 15 hours to 30 hours (S20);

Extracting the cheonnyeoncho extract using a solvent of 30 to 90% concentration of the dried cheonnyeoncho in step S20 (S30);

The step of reacting the extract of cheonnyeoncho in step S30 with a pectin-degrading enzyme at 15 to 35° C. for 30 minutes to 2 hours (S40);

Enzyme inactivation treatment step of 20 to 30 ℃, 1 hour to 2 hours of the extract of chrysanthemum reacted with the pectin-degrading enzyme of step S40 (S50);

Centrifuging the inactivated chrysanthemum extract of step S50 to prepare an enzyme-treated chrysanthemum extract (S60); and

Concentrating the enzyme-treated cheonnyeoncho extract of step S60 under reduced pressure at low temperature to prepare a cheonnyeoncho extract (S70); includes.

The cheonnyeoncho (Opuntia humifusa) may use any one or more selected from the group consisting of stems, flowers, seeds, fruits and roots.

It may further include the step of sectioning the millennium after washing with water.

Cheonnyeoncho (Opuntia hurnifusa) is a natural component factor with excellent moisturizing effect, and by including its extract as an active ingredient, it is possible to increase the moisturizing effect of the cosmetic composition.

The extraction method of step S30 may be prepared using commonly used hot water extraction method, solvent extraction method, countercurrent extraction method, ultrasonic extraction method, compression method, steam extraction method, etc., but is not limited thereto. Preferably, a solvent extraction method can be used. there is.

The solvent of step S30 may be any one or more selected from the group consisting of hexane, ethanol, methanol, distilled water, propanol, ammonia, ethylene, aqueous alcohol solution, ethyl acetate, butylene glycol, propylene glycol and dichloromethane, It is not limited thereto, and any solvent used in a conventional extraction method may be used.

Originally, cheonnyeoncho is known to have excellent moisturizing properties because it contains a large amount of antioxidants taxifolin, polyphenols, vitamins, and flavonoids as well as nutrients such as dietary fiber, minerals, amino acids, and mucopolysaccharides. Due to the viscosity, stickiness and viscosity were increased, so that it was difficult to use it as a cosmetic composition.

Therefore, the present invention performs the enzymatic treatment with a pectin-degrading enzyme as in step S40 in order to lower the high viscosity caused by the pectin contained in chrysanthemum.

The pectinase in step S40 is pectinase, pectinlyase, polygalacturonase, cellulase, beta-glucanase, xylanase, arabanase, beta-glucanase, amylase and a group consisting of hemicellulase It is preferably at least one selected from, but is not limited thereto.

It is preferable that the amount of the pectin-degrading enzyme of step S40 is 5 to 30 parts by weight based on 100 parts by weight of the extract of cheonnyeoncho in step S30.

When the pectin-degrading enzyme exceeds the range of the content, an overreaction may occur, resulting in problems such as a decrease in moisturizing factors and a decrease in active ingredients. When used as an ingredient, problems such as inducing the generation of toxic factors may occur by causing skin troubles and damage to the skin barrier, and by inducing the production of alcohol components when mixing raw materials.

As in step S40, in order to remove the enzyme from the extract of chrysanthemum that has been reacted with the pectin-degrading enzyme, inactivation treatment may be performed at 20 to 30 °C for 1 hour to 2 hours.

By centrifuging the inactivated chrysanthemum extract in step S50 for 10 to 20 minutes using a centrifuge, an enzyme-treated chrysanthemum extract can be prepared.

In addition, the present invention prepares a cheonnyeoncho extract by finally extracting the enzyme-treated cheonnyeoncho extract at low temperature and reduced pressure as in S60 in order to effectively lower the high viscosity caused by the pectin contained in the cheonnyeoncho.

When extracting cheonnyeoncho by concentration under reduced pressure at low temperature as in the present invention, the binding structure of pectin, which is the main component of cheonnyeoncho, is weakened by low pressure, so that the viscosity can be reduced.

In addition, since the extraction is carried out slowly under low temperature and low pressure conditions, the destruction of the extracted active ingredient can be minimized, and impurities can be effectively removed.

The conditions of the low-temperature vacuum concentration in step S70 are preferably a temperature of 30 to 80° C., a pressure of less than 1 atm, and 3 to 24 hours.

The pressure may be, more specifically, a pressure of 0.001 to less than 1 atm.

In addition, it provides a cheonnyeoncho extract prepared by the manufacturing method of the present invention.

In addition, there is provided a cosmetic composition comprising the extract of cheonnyeoncho produced by the manufacturing method of the present invention.

The cosmetic composition comprising the extract of chrysanthemum produced by the method of the present invention is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, Formulated with any one selected from the group consisting of essence, nutritional essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder and loose powder It may be formulated, but it is not limited thereto, and it can be formulated in any formulation conventionally prepared in the art.

In addition, the cosmetic composition containing the extract of chrysanthemum prepared by the manufacturing method of the present invention may be prepared and used as a composition in the form of an absorbent gel, pad, patch, sponge and mask pack as a formulation that can be applied to the skin, but is not limited thereto. does not

In addition, the cosmetic composition containing the extract prepared by the method of the present invention may be applied to the skin in a form that is applied to the skin, or may be applied in a form that is absorbed into the skin using a microneedle or the like.

In general, the cheonnyeoncho extract prepared by the production method of the present invention can lower the viscosity due to pectin, a problem of the conventional cheonnyeoncho extract by enzymatic treatment with pectin-degrading enzyme and concentration under reduced pressure at low temperature. Even when the extract is included in the cosmetic composition, stickiness and viscosity are reduced, so that the feeling of use can be improved.

On the other hand, in the case of glycerin and hyaluronic acid used as conventional cosmetic compositions, as the content is increased to increase the moisturizing effect, the viscosity increases and the feeling of use decreases. It can exhibit sufficient moisturizing effect without causing problems due to viscosity and stickiness.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1 Preparation of extract of chrysanthemum

After cutting the stems of chrysanthemum by washing with water, the thorns were removed and dried at room temperature 45° C. for 24 hours. The dried stems of chrysanthemum were extracted at a concentration of 60% using ethanol.

A pectin-degrading enzyme obtained by mixing 1ml of pectinase, arabanase, cellulase, beta-glucanase, hemicellulase, and Viscozyme L of Novozyme, which is a complex enzyme containing xylanase, and 9ml of Viscozyme L, 100% 15 parts by weight was added based on parts by weight, reacted at 25° C. for 1 hour, and after enzyme treatment, inactivated at 25° C. for 1 hour, and centrifuged for 15 minutes using a centrifuge.

After the centrifugation, the extracted extract was extracted using a rotary decompression concentrator at a temperature of 70° C. while maintaining a pressure of 0.5 atm or less for 4 hours to prepare a cheonnyeoncho extract.

Comparative Example 1

Except for the enzyme treatment process in Example 1, the remaining conditions were prepared in the same manner.

Comparative Example 2

Except for the extraction while maintaining the pressure of 1.0 atm in Example 1, the remaining conditions were prepared in the same manner.

Comparative Example 3

Except for extraction while maintaining the enzyme treatment process and pressure of 1.0 atm in Example 1, the remaining conditions were prepared in the same manner.

Evaluation Example 1 Functional evaluation

Component analysis was performed on polyphenols and flavonoids contained in the extract of chrysanthemum plant prepared by the method according to Examples 1 to 3, and the results are shown in Table 1 below.

Component analysis was measured by performing the Maksimovic method. For polyphenol component analysis, garlic acid (Sigma Aldrich) was used as a standard material, and absorbance was measured with light of a wavelength of 725 nm and evaluated, and flavonoids Component analysis was evaluated by adding aluminum chloride hexahydrate to the extract of cheonnyeoncho, and measuring the absorbance with light of a wavelength of 430 nm.

Polyphenols (mg/g) Flavonoids (mg/g) Example 1 5.19 ±0.19 1.33 ±0.09 Comparative Example 1 3.36 ±0.20 0.94 ±0.11 Comparative Example 2 2.87 ±0.11 0.53 ±0.09 Comparative Example 3 2.23 ±0.16 0.38 ±0.10

As shown in Table 1, it was confirmed that the polyphenols and flavonoids contained in the extract of Example 1 were higher, and through this, the content of the active ingredient due to the marked improvement in extraction efficiency in the case of the extract of the present invention. It was found that the functional properties such as moisturizing power were high.

Evaluation Example 2 Antioxidant effect evaluation

DPPH free radical scavenging ability, nitric oxide (NO) scavenging activity and reducing power were analyzed in order to evaluate the antioxidant effect of the extracts of Millennials of Examples 1 to 3, and the results are shown in Table 2 below.

(1) DPPH free radical scavenging ability

In order to measure the DPPH free radical scavenging ability, 2 mL of a methanol solution containing 100 μM DPPH was added to 100 μL of the extract of Millennial plant of Examples 1 to 3, shaken, and left at room temperature for 10 minutes, followed by a UV-Vis spectrophotometer (SINCO S-3100) , Korea) was used to measure the absorbance at 517 nm to measure the decrease in absorbance due to reduction of DPPH. After measuring the relative DPPH free radical scavenging activity by measuring the standard material together, the IC50 (the concentration of the sample that inhibits 50% of the DPPH free radicals) calculated by regression analysis was shown. Ascorbic acid was used as a standard material.

DPPH free radical scavenging activity was calculated according to the following formula, and the results are shown in Table 2 below.

DPPH free radical scavenging activity (%) = (1-A/B)x100

A: Absorbance of Examples 1 to 3 at 517 nm, B: Absorbance of a standard material at 517 nm

(2) NO scavenging activity

In order to measure the NO scavenging activity, 100 μL of the extract of cheonnyeoncho from Examples 1 to 3 was reacted with the griess reagent, and then the absorbance was measured at 540 nm to calculate the NO inhibition rate. The IC50 (concentration of the sample that inhibits 50% of NO) calculated by regression analysis after measuring the relative NO scavenging activity of the sample was shown by measuring the control group without the sample. Ascorbic acid was used as a standard material.

(3) reducing power

In order to measure the reducing power, 2.5 mL of 0.2M sodium phosphate buffer (pH 6.6) and 2.5 mL of 1% potassium ferricyanide were added to 2.5 mL of the extract of ch. peony extract of Examples 1 to 3 dissolved in distilled water, and then reacted at 50 ° C. for 20 minutes. made it After adding 2.5 mL of 10% trichloroacetic acid, centrifuge the reaction solution at 3,000 rpm for 10-15 minutes, take 5 mL of the supernatant, mix with 5 mL of distilled water, add 1 mL of 0.1% ferric chloride (FeCI 3 ·H 2 O), and absorb absorbance at 700 nm showed the reducing power. Ascorbic acid was used as a standard material.

DPPH scavenging ability NO scavenging activity reducing power Example 1 0.67 ±0.08 0.17 ±0.06 3.43 ±0.23 Comparative Example 1 0.95 ±0.10 0.15 ±0.03 2.18 ±0.21 Comparative Example 2 0.79 ±0.28 0.12 ±0.02 1.77 ±0.09 Comparative Example 3 1.19 ±0.24 0.11 ±0.01 1.47 ±0.12 ascorbic acid 10.19 ±1.05 6.88 ±0.71 1.88 ±0.14

As shown in Table 2, it was confirmed that the DPPH scavenging activity, NO scavenging activity, and reducing power of the chrysanthemum extract of Example 1 were significantly superior to those of Comparative Examples 1 to 3 and ascorbic acid. Through this, it was found that the antioxidative ability of the extract prepared by the present invention was improved.

Evaluation Example 3 Viscosity Comparison

The yield strength was analyzed to compare the viscosities of the extracts prepared by the method according to Examples 1 to 3, and the results are shown in Table 3 below.

Yield force analysis was evaluated by measuring the shear stress while changing the shear rate from 0 to 300s-1 of the extract according to the concentration at 20 ℃ using a viscosity analyzer.

Yield Force (Pa) Example 1 13.22 ±0.49 Comparative Example 1 19.08 ±0.48 Comparative Example 2 20.25 ±0.33 Comparative Example 3 23.61 ±0.51

As shown in Table 3, it was confirmed that the yield strength of the cheonnyeoncho extract of Example 1 was the lowest, and it was confirmed that the viscosity of the cheonnyeoncho extract was significantly reduced through such a reduction in the yield strength.

Claims (8)

Washing the cheonnyeoncho (Opuntia humifusa) (S10);
Drying the millennium plant washed with water in the step S10 at 30 to 60° C. for 15 hours to 30 hours (S20);
Extracting the cheonnyeoncho extract using a solvent of 30 to 90% concentration of the dried cheonnyeoncho in step S20 (S30);
The step of reacting the extract of cheonnyeoncho in step S30 with a pectin-degrading enzyme at 15 to 35° C. for 30 minutes to 2 hours (S40);
Enzyme inactivation treatment step of 20 to 30 ℃, 1 hour to 2 hours of the extract of chrysanthemum reacted with the pectin-degrading enzyme of step S40 (S50);
Centrifuging the inactivated chrysanthemum extract of step S50 to prepare an enzyme-treated chrysanthemum extract (S60); and
Concentrating the enzyme-treated cheonnyeoncho extract of step S60 under reduced pressure at a low temperature to prepare a cheonnyeoncho extract (S70); including
A method for producing an extract of chrysanthemum.
The method according to claim 1,
The method for producing a cheonnyeoncho extract, characterized in that the cheonnyeoncho (Opuntia humifusa) is any one or more selected from the group consisting of stems, flowers, seeds, fruits and roots.
The method according to claim 1,
The solvent of step S30 is hexane, ethanol, methanol, distilled water, propanol, ammonia, ethylene, an aqueous alcohol solution, ethyl acetate, butylene glycol, propylene glycol and dichloromethane, characterized in that any one or more selected from the group consisting of cheonnyeoncho Method for preparing the extract.
The method according to claim 1,
The pectinase in step S40 is pectinase, pectinlyase, polygalacturonase, cellulase, beta glucanase, xylanase, arabanase, beta-glucanase, a group consisting of amylase and hemicellulase A method of producing a cheonnyeoncho extract, characterized in that at least one selected from
The method according to claim 1,
The pectin-degrading enzyme of step S40 is a method of producing a cheonnyeoncho extract, characterized in that 5 to 30 parts by weight with respect to 100 parts by weight of the cheonnyeoncho extract of the step S30.
The method according to claim 1,
The conditions of the low-temperature reduced pressure concentration of the step S70 are a temperature of 30 to 80 ℃, a pressure of less than 1 atm, and 3 to 24 hours.
A cheonnyeoncho extract prepared by the method of any one of claims 1 to 6.
A cosmetic composition comprising the extract of claim 7 .

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