KR20220051579A - Brain health improving formulation containing Pulsed Electric Field treated broccoli sprout extract - Google Patents

Abstract

The present invention relates to a brain health function improver composition containing a broccoli sprout extract that helps a memory increase, has an excellent neuronal cell death inhibitory effect and an excellent neuroinflammation suppression effect, and helps recovery of neurotransmitters and synaptic plasticity. The brain health function improver composition contains a broccoli sprout extract, which is treated with a pulsed electric field and reacted with myrosinase to produce sulforaphane, to especially help a memory increase, have an excellent neuronal cell death inhibitory effect and an excellent neuroinflammation suppression effect, and show a very good effect of helping recovery of neurotransmitters and synaptic plasticity.

Description

Brain health improving formulation containing Pulsed Electric Field treated broccoli sprout extract}

The present invention relates to a brain health function improving composition containing a broccoli sprout extract treated with a pulsed electric field, that is, a brain health function improving agent composition containing a broccoli sprout extract, particularly helpful in improving memory, inhibiting neuronal cell death, and It is excellent in neuroinflammation inhibitory effect, and relates to a brain health function improving composition containing a broccoli sprout extract that helps to restore neurotransmitters and synaptic plasticity.

Human memory is exerted by the action of neurons in the brain, and brain neurons form and store memories through the process in which various neurotransmitters are transmitted through synapses, which are gaps between cells.

Various studies are being carried out to improve human memory, for example, Patent Document 1 below is a pharmaceutical composition containing cheongpi jingokgo as an active ingredient to protect nerve cells and improve memory, and functional food containing cheongpi gyeongokgo. is starting

On the other hand, in order to improve memory, it is necessary to effectively inhibit the death of nerve cells involved in memory, inhibit neuroinflammation that can lower memory, and also to restore neurotransmitters and synaptic plasticity directly related to memory. .

Therefore, in order to improve the effect of memory improvement, it effectively inhibits the death of nerve cells involved in memory and at the same time inhibits neuroinflammation that can decrease memory, and also improves the neurotransmitter and synaptic plasticity directly related to memory. There is an urgent need for the development of a recovery composition.

Patent Document 1: Korean Patent Publication No. 10-2015-0121788 (2015.10.30)

Therefore, in the present invention, in order to solve the above problems, a composition comprising a broccoli sprout extract and Jeju lava seawater is used to effectively inhibit the death of nerve cells involved in memory and at the same time inhibit neuroinflammation that can decrease memory, In addition, it was discovered that memory can be remarkably improved by restoring neurotransmitters and synaptic plasticity directly related to memory, and the present invention has been completed based on this.

The brain health function improving agent composition according to an embodiment of the present invention includes an extract of broccoli sprouts grown with lava seawater.

In the brain health function improving agent composition according to an embodiment of the present invention, the broccoli sprout extract is processed by applying pulse energy to the broccoli sprout (S100); Preparing a sprout juice by squeezing the broccoli sprouts treated with pulse energy (S200); Preparing the sprout fermented alcohol extract by mixing and reacting myrosinase with the sprout juice and extracting it with fermented alcohol (S300); preparing a broccoli sprout concentrate by stirring the sprout fermented alcohol extract and concentrating the stirred broccoli sprout fermented alcohol extract under reduced pressure (S400); Preparing the broccoli sprout extract by drying the broccoli sprout concentrate (S500); and grinding the dried broccoli sprouts into powder (S600).

In the brain health function improving agent composition according to an embodiment of the present invention, the composition is characterized in that it is a memory improving agent.

In the brain health function improving agent composition according to an embodiment of the present invention, the composition is characterized in that it is a neuronal cell death inhibitor.

In the brain health function improving agent composition according to an embodiment of the present invention, the composition is characterized in that it is a neuroinflammation inhibitor.

In the brain health function improving agent composition according to an embodiment of the present invention, the composition is characterized in that the composition is a neurotransmitter and a synaptic plasticity recovery agent.

Brain health function improving composition according to the present invention contains a pulsed electric field-treated broccoli sprout extract, which is particularly helpful in improving memory, has excellent neuronal apoptosis inhibitory effect and neuroinflammation inhibitory effect, neurotransmitter and synaptic plasticity recovery It has an excellent effect conducive to

1 is a graph showing the component profile of the broccoli sprout extract prepared according to the present invention.
2 shows the standards and test method validation values of sulforaphane and glucoraphanine, which are major components of the broccoli sprout extract prepared through the above process.
3 shows the results of analysis of the main component content of the broccoli sprout extract prepared through the above process.
4 to 6 show the heavy metal and microorganism test report of the broccoli sprout extract prepared through the above process.

Before describing the present invention in more detail, the terms or words used in the present specification and claims should not be limited to their ordinary or dictionary meanings, and the concept of terms should be properly explained in order to best describe the invention. It should be interpreted as meaning and concept consistent with the technical idea of the present invention based on the principle that it can be defined in Therefore, the configuration of the embodiments described in the present specification is only one preferred example of the present invention, and does not represent all the technical spirit of the present invention, so various equivalents and modifications that can be substituted for them at the time of the present application It should be understood that there may be

Hereinafter, preferred embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily practice the present invention.

The brain health function improving agent composition according to an embodiment of the present invention includes an extract of broccoli sprouts grown in lava seawater.

The brain health function improving agent composition according to an embodiment of the present invention can be mixed with various pharmaceutical carriers and processed into a tablet form, or it can be ingested in a pharmaceutical capsule, and a certain amount in a liquid state in a bottle It is also possible to take each.

On the other hand, it is also possible to ingest the brain health function improving agent composition according to an embodiment of the present invention as a food by adding it to various foods.

The present inventors use a composition comprising a broccoli sprout extract grown with lava seawater to effectively inhibit the death of nerve cells involved in memory and at the same time inhibit neuroinflammation, which can decrease memory, and also directly related to memory. It was discovered that memory can be remarkably improved by restoring neurotransmitters and synaptic plasticity, and various experiments were conducted to verify this.

In particular, in order to prepare the brain health function improving composition of the present invention, the present inventors used a method of growing broccoli sprouts using Jeju lava seawater. It was confirmed that the memory improvement effect of

In addition, in the present invention, it was found that active ingredients that can improve the effect of memory improvement can be extracted very efficiently by extracting and using the broccoli sprout extract as follows.

The broccoli sprout extract used in the present invention is carried out through the following process.

That is, the broccoli sprout extract is processed by applying pulse energy to the broccoli sprout (S100); Preparing a sprout juice by squeezing the broccoli sprouts treated with pulse energy (S200); Preparing the sprout fermented alcohol extract by mixing and reacting myrosinase with the sprout juice and extracting it with fermented alcohol (S300); preparing a broccoli sprout concentrate by stirring the sprout fermented alcohol extract and concentrating the stirred broccoli sprout fermented alcohol extract under reduced pressure (S400); Preparing the broccoli sprout extract by drying the broccoli sprout concentrate (S500); and grinding the dried broccoli sprout extract into a powder (S600).

The step (S100) of processing by applying pulse energy to the broccoli sprout is a process of applying a pulsed electric field (PEF), for example, the broccoli using a pulsed electric field (PEF) of BK Bio Inc. It is a process that destroys the cell membrane of the sprout.

The step (S100); is, for example, by operating a pulse electric field (PEF) of BK Bio Co., Ltd. is performed through the process of applying the energy to the broccoli sprout for about 1 minute to 1 hour.

In some cases, the step (S100) may be replaced by the process of crushing broccoli sprouts.

Next, a step (S200) of preparing a sprout juice by squeezing the broccoli sprout treated with pulse energy is performed, and through this process, it is possible to further increase the extraction efficiency of the broccoli sprout extract.

Next, a step (S300) of preparing the sprout fermented alcohol extract by mixing and reacting myrosinase with the sprout juice and extracting it with fermented alcohol (S300) is performed.

Myrosinase can be reacted at a temperature of 30 to 40° C. for 10 minutes to 10 hours.

Next, the step (S400) of preparing the broccoli sprout concentrate by stirring the sprout fermented alcohol extract and concentrating the broccoli sprout fermented alcohol extract under reduced pressure is performed.

The stirring process may be performed at a temperature of 20 to 50° C. for about 3 hours.

The broccoli sprout fermented alcohol extraction process can be performed for about 10 minutes to 12 hours.

The reduced pressure concentration may be performed at a temperature of about 40°C.

Next, the broccoli sprout extract concentrate is dried to prepare a dried broccoli sprout extract (S500); is performed.

Finally, pulverizing the dried broccoli sprout extract to make a powder (S600); is performed, and it is possible to prepare a powder filtered with about 60 mesh.

The broccoli sprout extract prepared through this process has a high yield of about 3% by weight.

1 shows a graph of the component profile of the broccoli sprout extract prepared through this process.

Component analysis was performed by Q-Orbitrap Mass Spectrometry of BK Bio Co., Ltd., and all peaks of each extract were identified.

2 shows the standards and test method validation values of sulforaphane and glucoraphanine, which are major components of the broccoli sprout extract prepared through the above process.

Figure 3 shows the main component content analysis result of the broccoli sprout extract prepared through the above process.

4 to 6 show the heavy metal and microorganism test report of the broccoli sprout extract prepared through the above process.

On the other hand, the brain health function improving agent composition according to the present invention can be used as a food and at the same time as a medicine that exhibits the following effects.

That is, the brain health function improving agent composition according to the present invention can be used as a memory improving agent.

In addition, the brain health function improving agent composition according to the present invention can be used as a nerve cell death inhibitor.

In addition, the brain health function improving agent composition according to the present invention can be used as a neuroinflammation inhibitor.

In addition, the brain health function improving agent composition according to the present invention can be used as a neurotransmitter and synaptic plasticity recovery agent.

Hereinafter, experiments performed to confirm various effects according to an embodiment of the present invention will be described.

[Preparation Example 1] - Preparation of broccoli sprout extract

The cells of broccoli sprouts were destroyed by applying pulse energy to 50 kg of broccoli sprouts grown in Jeju lava seawater for about 1 minute using a pulse electric field (PEF) from BK Bio. Thereafter, 25 g of myrosinase was mixed with the broccoli sprouts, whose cells were destroyed by the pulse energy treatment, and reacted at a temperature of about 37° C. for about 2 hours, followed by extraction with sulforaphane-enriched broccoli sprout fermented alcohol. Then, the broccoli sprout fermented alcohol extract thus extracted was stirred for about 3 hours, and then, the stirred broccoli sprout fermented alcohol extract was concentrated under reduced pressure at a temperature of about 40° C. to prepare a broccoli sprout concentrate. Next, the broccoli sprout concentrate was dried to prepare a dried broccoli sprout extract, and finally, the dried broccoli sprout was pulverized to prepare a powder filtered through about 60 mesh.

[Production Example 2]

Broccoli sprouts were prepared by mechanically pulverizing 50 kg of broccoli sprouts grown with Jeju lava seawater.

[Preparation Example 3] - Myrosinase (myrosinase) untreated

The cells of broccoli sprouts were destroyed by applying pulse energy to 50 kg of broccoli sprouts grown in Jeju lava seawater for about 30 minutes using a pulse electric field (PEF) from BK Bio. Thereafter, the broccoli sprouts, whose cells were destroyed by the pulse energy treatment as described above, were left in the air, and myrosinase contained in the broccoli sprouts reacted with glucoraphanin to extract the enzyme reaction solution in which sulforaphane was produced. Then, the broccoli sprout enzyme reaction solution extracted in this way was stirred for about 3 hours, and then, the stirred broccoli sprout enzyme reaction solution was concentrated under reduced pressure at a temperature of about 40° C. to prepare a broccoli sprout concentrate. Next, the broccoli sprout concentrate was dried to prepare a dried broccoli sprout extract, and finally, the dried broccoli sprout extract was pulverized to prepare a powder filtered through about 60 mesh.

[Preparation Example 4] - Preparation of broccoli sprout extract

The cells of broccoli sprouts were destroyed by applying pulse energy to 50 kg of broccoli sprouts grown with tap water for about 1 minute using a pulse electric field (PEF) of BK Bio. Thereafter, 25 g of myrosinase was mixed with the broccoli sprouts whose cells were destroyed by the pulse energy treatment as described above and reacted at a temperature of about 37° C. for about 2 hours, and then the broccoli sprouts were extracted with fermented alcohol. Then, the broccoli sprout fermented alcohol extract thus extracted was stirred for about 3 hours, and then, the stirred broccoli sprout fermented alcohol extract was concentrated under reduced pressure at a temperature of about 40° C. to prepare a broccoli sprout extract concentrate. Next, the broccoli sprout extract concentrate was dried to prepare a broccoli sprout extract, and finally, the dried broccoli sprout was ground to prepare a powder filtered through about 60 mesh.

[Preparation Example 5] - Preparation of broccoli sprout extract

By applying pulse energy to 50 kg of broccoli sprouts grown in drinking spring water for about 1 minute using a pulse electric field (PEF) of BK Bio, the cells of broccoli sprouts were destroyed. Thereafter, 25 g of myrosinase was mixed with broccoli sprouts, whose cells were destroyed by the pulse energy treatment, and reacted at a temperature of about 37° C. for about 2 hours. The broccoli sprout enzyme reaction solution was extracted with fermented alcohol. Then, the broccoli sprout fermented alcohol extract thus extracted was stirred for about 3 hours, and then, the stirred broccoli sprout fermented alcohol extract was concentrated under reduced pressure at a temperature of about 40° C. to prepare a broccoli sprout concentrate. Next, the broccoli sprout concentrate was dried to prepare a dried broccoli sprout extract, and finally, the dried broccoli sprout extract was pulverized to prepare a powder filtered through about 60 mesh.

[Preparation Example 6] - Preparation of broccoli sprout extract

The cells of broccoli sprouts were destroyed by applying pulse energy to 50 kg of broccoli sprouts grown in deep sea water for about 1 minute using a pulse electric field (PEF) from BK Bio. Thereafter, 25 g of myrosinase was mixed with broccoli sprouts, whose cells were destroyed by the pulse energy treatment, and reacted at a temperature of about 37° C. for about 2 hours. The broccoli sprout enzyme reaction solution was extracted with fermented alcohol. Then, the broccoli sprout extract and fermented alcohol thus extracted was stirred for about 3 hours, and then, the stirred broccoli sprout extract and fermented alcohol was concentrated under reduced pressure at a temperature of about 40° C. to prepare a broccoli sprout concentrate. Next, the broccoli sprout concentrate was dried to prepare a dried broccoli sprout extract, and finally, the dried broccoli sprout extract was pulverized to prepare a powder filtered through about 60 mesh.

Pulsed electric field (PEF) treatment conditions of BK Bio Co., Ltd. used in the present invention are 0.1 to 200 seconds with an electric field of 0.1 to 15 kV/cm, a frequency of 1 to 120 Hz, and an energy of 1 to 30 kJ in a liquid medium containing sprouts. It is possible to perform from 1 to 100 times during the period.

On the other hand, the pulse electric field (PEF) treatment conditions of BK Bio Co., Ltd. used in Preparation Examples 1 to 6 were 50 times for 100 seconds with an electric field of 10 kV/cm, a frequency of 80 Hz, and an energy of 15 kJ in the liquid medium containing the sprouts. carried out.

[Example 1]

5 kg of the broccoli sprout extract prepared in Preparation Example 1 was evenly mixed with dextrin as an excipient to prepare a brain health function improving composition according to the present invention.

[Comparative Example 1] - Broccoli sprout pulverized product

5 kg of the ground broccoli sprout prepared in Preparation Example 2 was evenly mixed with dextrin as an excipient to prepare a mixed composition.

[Comparative Example 2] - Broccoli sprout juice

5 kg of the composition prepared in Preparation Example 3 was evenly mixed with dextrin as an excipient to prepare a mixed composition.

[Comparative Example 3]

5 kg of the broccoli sprout extract prepared in Preparation Example 4 was evenly mixed with dextrin as an excipient to prepare a mixed composition.

[Comparative Example 4]

5 kg of the broccoli sprout extract prepared in Preparation Example 5 was evenly mixed with dextrin as an excipient to prepare a mixed composition.

[Comparative Example 5]

5 kg of the broccoli sprout extract prepared in Preparation Example 6 was evenly mixed with dextrin as an excipient to prepare a mixed composition.

The following experiment was performed using the brain health function improving agent composition prepared in Example 1.

[Experiment 1] - Evaluation of neuronal cell death inhibitory effect

The neuronal cell death inhibitory effect was evaluated through the following experimental methods (1) to (3).

(1) Western Blot Analysis: Caspase-3 and Cleaved Caspase-3 protein expression measurement experiment was performed, and it was confirmed that the expression levels of Caspase-3 and Cleaved Caspase-3 proteins were each increased 10 times compared to the untreated control group.

(2) Western Blot Analysis: An experiment was performed to measure the expression of PARP and cleaved PARP proteins, and it was confirmed that the expression levels of PARP and cleaved PARP proteins increased 5 times, respectively, compared to the untreated control group.

(3) Western Blot Analysis: An experiment was performed to measure the expression of Bax and Bcl-2 proteins, and it was confirmed that the expression of Bax and Bcl-2 proteins increased about 7 times compared to the untreated control group.

[Experiment 2] - presence or absence of neurotoxicity

As a result of treating the composition of Example 1 up to 24 and 48 hours, cytotoxicity was not observed.

[Experiment 3] - Cell growth rate test

As a result of treatment with the composition of Example 1 for 48 hours, it was confirmed that the cell growth rate increased at 1250 μg/mL.

Through the above experiments 1 to 3, it was possible to confirm the change in the protein expression of the factors related to apoptosis inhibition by the composition of Example 1, and cells at a concentration of 10, 50 and 100 g/ml that do not show cytotoxicity by the composition of Example 1 It was confirmed that the apoptosis inhibitory effect was exhibited.

[Experiment 4] - Evaluation of inhibitory effect on neuroinflammation

The neuroinflammation inhibitory effect was evaluated through the following experiments (1) to (3).

(1) Western Blot Analysis: NF-κB protein expression was measured and migration to the cell nucleus was observed.

(2) Western Blot Analysis: It was observed that the expression of COX-2 protein increased.

(3) Western Blot Analysis: MAPKs (ERK, JNK, p38MAPK) protein expression was observed to increase.

[Experiment 5]

By treating the composition of Example 1, it was confirmed that the nuclear localization of NF-κB, COX-2 protein expression, and phosphorylation of MAPKs (ERK, p38 MAPK) proteins increased in a cell inflammatory response in a concentration-dependent manner compared to the untreated control group. , it was confirmed that there was a significant difference in the inhibitory effect of neuronal inflammatory response at concentrations of 10, 50, and 100 μg/mL at the levels of p<0.05, p<0.01, and p<0.001 when compared with the untreated group.

[Experiment 6] - Neurotransmitter and synaptic plasticity recovery evaluation

Example 1 During the treatment of the composition, neurotransmitters and synaptic plasticity recovery evaluation were performed through the experiments of (1) to (3) below.

(1) Acetylcholinesterase Assay: Acetylcholinesterase activity was observed to increase.

(2) ELISA (Enzyme-Linked Immunosorbent Assay): An increase in the amount of BDNF released from cells was measured.

(3) Western Blot Analysis: It was observed that CREB and p-CREB protein expression increased.

[Experiment 7]

Example 1 It was confirmed that when the composition was treated, the activity of Acetylcholinesterase was inhibited in Aβ-induced neurons compared to the untreated control, and the amount of brain-derived neurotrophic factor (BDNF) released from the cells was increased in a concentration-dependent manner.

[Experiment 8]

When the composition of Example 1 was treated, the transcriptional activity of CREB, a neurotransmitter-related transcription factor, was increased in a concentration-dependent manner as compared to the untreated control group. It was confirmed that there was a significant difference in the level of recovery at p <0.05, p <0.01, and p <0.001 levels.

[Experiment 9]

Example 1 The composition of Example 1 was orally administered to 7 elderly people (male: 3, 4 female) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For the memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and show 10 randomly among the number memorization ability evaluation to evaluate how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation to evaluate how many of them can be remembered after 10 seconds, and the results are shown in Table 1 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/2 2/4 3/3 3/2 3/4 2/3 3/2 2 days 2/2 3/3 3/3 3/3 3/3 3/2 3/2 3 days 3/2 2/5 3/4 3/3 3/4 3/3 3/4 4 days 2/3 4/4 4/5 3/4 4/4 3/4 5/5 5 days 4/5 5/6 6/5 5/4 4/4 3/4 6/7 6 days 5/5 6/5 5/4 5/5 5/6 5/5 7/7 7 days 5/6 6/6 6/5 6/5 6/5 5/6 7/7 8 days 6/5 6/7 5/6 5/6 6/6 6/5 7/8 9 days 6/6 6/6 5/6 6/5 6/6 7/5 8/7 10 days 6/5 7/6 6/5 6/6 6/6 7/5 8/8 11 days 6/7 7/7 6/6 7/7 7/6 7/5 7/8 12 days 7/6 8/7 7/7 7/8 6/7 7/6 7/8 13 days 7/7 6/7 6/6 8/7 7/7 7/6 8/7 14 days 7/5 6/7 7/5 7/7 6/6 7/7 7/8 15th 6/7 7/7 7/6 7/6 7/7 7/7 8/7 16 days 5/7 8/6 7/7 7/7 7/8 7/6 7/8 17 days 7/7 7/8 6/7 7/7 8/7 7/6 8/8 18 days 8/7 7/7 7/7 7/8 7/7 7/7 8/7 19 days 7/8 8/7 8/7 8/7 7/7 8/8 9/8 20 days 8/7 7/8 7/7 8/7 8/7 7/8 8/7

(Number memorization skill evaluation score/figure memorization skill evaluation score)

Looking at the results in Table 1, it can be seen that the composition according to the present invention is clearly helpful in improving memory.

[Experiment 10]

Comparative Example 1 The composition was orally administered to 7 elderly people (male: 3, 4 females) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and display 10 randomly among the number memorization ability evaluation, which evaluates how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation to evaluate how many of them can be remembered after 10 seconds, and the results are shown in Table 2 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/3 3/4 3/3 3/2 3/4 2/3 3/2 2 days 4/3 3/4 3/3 3/3 3/3 3/2 3/2 3 days 3/3 2/4 3/4 3/3 3/4 3/3 3/4 4 days 3/3 4/3 3/3 3/4 4/4 3/4 3/3 5 days 4/3 3/4 3/4 3/3 4/4 3/4 3/2 6 days 3/4 4/3 3/3 3/3 4/4 3/4 3/3 7 days 4/3 3/4 3/4 3/4 3/4 3/3 3/2 8 days 3/4 4/3 3/4 3/4 3/4 2/3 3/3 9 days 3/4 3/4 3/3 3/3 4/4 3/4 3/3 10 days 4/3 3/4 3/3 3/3 3/4 3/3 3/3 11 days 4/3 4/3 3/4 3/4 4/3 3/3 3/2 12 days 4/3 3/4 3/3 4/4 3/4 3/3 3/3 13 days 3/3 4/4 3/4 3/3 3/4 3/4 3/3 14 days 3/4 3/4 4/3 4/3 4/4 3/3 3/2 15th 4/3 3/4 3/4 3/4 4/3 3/4 3/3 16 days 3/4 3/4 3/4 3/3 3/4 3/3 3/2 17 days 3/4 4/3 3/3 3/4 4/4 3/4 3/3 18 days 3/3 4/3 3/4 3/3 3/4 3/3 3/2 19 days 3/4 3/3 3/4 3/4 4/4 3/4 3/3 20 days 3/4 4/3 3/4 3/3 3/4 4/3 3/2

(Number memorization skill evaluation score/figure memorization skill evaluation score)

[Experiment 11]

Comparative Example 2 The composition was orally administered to 7 elderly people (male: 3, 4 females) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For the memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and show 10 randomly among the number memorization ability evaluation to evaluate how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation to evaluate how many of them can be remembered after 10 seconds, and the results are shown in Table 3 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/3 2/4 3/3 3/3 3/4 2/3 3/2 2 days 2/3 3/3 3/3 3/3 3/3 3/4 3/3 3 days 3/4 3/4 4/4 3/3 3/4 3/3 3/4 4 days 3/3 4/2 3/3 3/3 4/3 3/4 3/4 5 days 4/3 3/4 3/4 3/3 4/3 3/4 3/2 6 days 3/3 3/3 4/3 3/4 4/4 3/4 3/3 7 days 4/3 3/4 3/4 3/4 3/4 4/3 3/2 8 days 3/3 4/3 3/4 3/4 3/4 2/3 3/3 9 days 3/4 3/4 3/3 3/4 4/3 3/4 3/4 10 days 3/3 3/4 4/3 3/3 3/4 3/3 3/3 11 days 4/3 4/3 3/4 3/4 4/3 3/3 3/4 12 days 4/3 3/4 3/3 4/4 3/4 4/3 3/3 13 days 3/4 4/3 3/3 3/3 3/4 3/4 3/3 14 days 3/4 3/4 4/3 4/3 4/2 3/3 3/2 15th 4/4 3/3 3/3 3/4 4/3 3/4 3/3 16 days 3/4 3/4 3/4 3/4 3/4 4/3 3/3 17 days 3/4 4/3 3/3 3/4 3/4 3/4 3/4 18 days 3/4 4/3 3/3 3/3 3/4 3/3 3/2 19 days 3/4 3/4 3/4 3/3 3/4 4/4 4/3 20 days 3/4 4/3 3/4 3/3 3/4 4/3 3/2

(Number memorization skill evaluation score/figure memorization skill evaluation score)

[Experiment 12]

Comparative Example 3 The composition was orally administered to 7 elderly people (male: 3, 4 females) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For the memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and show 10 randomly among the number memorization ability evaluation to evaluate how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation, which evaluates how many of them can be remembered after 10 seconds, and the results are shown in Table 4 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/3 2/4 3/3 3/3 3/4 2/3 3/2 2 days 2/3 3/3 3/3 3/3 3/3 3/4 3/3 3 days 3/4 3/4 4/4 3/3 3/4 3/3 3/4 4 days 3/3 4/2 3/3 3/3 4/3 3/4 3/4 5 days 4/4 3/4 3/4 3/3 4/3 3/4 3/2 6 days 4/4 3/3 4/3 3/4 4/4 3/4 3/3 7 days 5/5 6/6 5/5 5/5 6/6 5/5 5/5 8 days 5/5 5/5 5/5 6/4 5/5 4/6 6/6 9 days 5/4 6/6 6/5 5/5 6/4 6/5 5/5 10 days 5/5 5/5 5/5 5/5 5/5 5/5 6/4 11 days 6/5 6/6 4/5 5/4 6/4 6/4 6/5 12 days 5/6 5/5 6/3 3/6 5/5 5/5 5/5 13 days 5/5 5/6 5/5 5/5 6/4 6/6 4/5 14 days 6/5 5/5 4/6 4/5 5/5 5/5 5/5 15th 6/5 6/4 5/5 5/5 5/5 5/5 6/5 16 days 5/5 5/5 5/4 6/5 6/4 6/3 5/5 17 days 6/6 6/5 5/5 5/5 5/5 4/5 6/4 18 days 6/5 6/5 6/4 5/4 5/6 6/5 4/6 19 days 6/6 5/6 4/6 6/5 4/6 6/4 4/6 20 days 5/6 6/5 5/5 4/6 4/6 4/5 4/5

(Number memorization skill evaluation score/figure memorization skill evaluation score)

[Experiment 13]

Comparative Example 4 The composition was orally administered to 7 elderly people (male: 3, 4 female) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For the memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and show 10 randomly among the number memorization ability evaluation to evaluate how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation to evaluate how many of them can be remembered after 10 seconds, and the results are shown in Table 5 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/3 2/4 3/3 3/3 3/4 2/3 3/2 2 days 3/3 3/3 4/3 3/3 3/2 3/4 3/3 3 days 3/4 3/4 4/4 3/4 3/4 3/4 4/4 4 days 3/3 4/2 3/3 4/3 3/3 3/4 3/4 5 days 4/4 3/4 3/5 3/3 4/3 3/3 3/2 6 days 4/4 3/3 4/3 3/4 4/3 3/4 3/3 7 days 5/5 6/5 5/4 4/5 6/6 5/5 5/5 8 days 5/5 5/5 5/5 6/4 5/5 4/6 6/4 9 days 5/4 6/5 6/5 5/5 5/4 6/5 5/5 10 days 5/4 5/5 5/4 4/5 5/5 5/5 6/4 11 days 6/5 6/6 4/5 5/4 6/4 5/4 4/5 12 days 5/5 5/5 6/3 3/6 5/4 5/5 5/5 13 days 5/5 5/6 5/5 5/5 6/4 6/5 4/5 14 days 4/5 5/5 4/6 4/4 4/5 5/5 5/5 15th 5/5 6/4 4/5 5/5 5/5 5/4 6/4 16 days 5/5 5/5 5/4 6/5 5/4 6/3 5/5 17 days 6/6 5/5 5/4 5/4 5/5 4/5 6/4 18 days 6/5 6/5 5/4 5/5 5/5 4/5 4/4 19 days 6/6 5/6 4/5 6/5 4/5 6/4 4/6 20 days 5/6 4/5 5/5 4/5 4/5 4/4 4/5

(Number memorization score/figure memorization score)

[Experiment 14]

The composition of Comparative Example 5 was orally administered to 7 elderly people (male: 3, 4 female) without obvious symptoms of dementia 3 times a day (20 ml each) for 30 days, and the effect on the memory of the participants was evaluated. (Participants 1 to 3: Male, Participants 4 to 7: Female).

For the memory evaluation, randomly show 10 of the number boards with numbers from 1 to 20, and show 10 randomly among the number memorization ability evaluation to evaluate how many of them can be remembered after 10 seconds, and the drawing board with various animal pictures. , was performed through picture memory evaluation to evaluate how many of them can be remembered after 10 seconds, and the results are shown in Table 6 below (numerical memorization power evaluation score/picture memorization power evaluation score) (out of 5 points).

division Participant 1 Participant 2 Participant 3 Participant 4 Participant 5 Participant 6 Participant 7 1 day 3/3 2/4 3/3 3/3 3/4 2/3 3/2 2 days 3/4 3/4 4/3 4/3 3/3 3/4 4/3 3 days 3/4 4/4 4/4 3/4 3/4 4/4 4/4 4 days 3/4 4/2 4/3 4/3 4/3 3/4 3/4 5 days 4/4 3/4 3/5 4/3 4/3 3/3 3/2 6 days 4/4 3/3 4/3 3/4 4/3 3/4 3/3 7 days 5/5 6/5 5/4 4/5 6/5 5/4 5/4 8 days 4/5 5/5 5/4 6/4 5/5 4/6 5/4 9 days 5/4 6/5 6/5 5/5 5/4 6/5 5/5 10 days 5/4 5/5 5/4 4/5 5/4 5/5 6/4 11 days 6/5 6/5 4/5 5/4 6/4 5/4 4/5 12 days 5/6 5/5 5/3 4/6 5/4 4/5 5/5 13 days 5/5 5/6 5/5 5/5 6/4 6/5 4/6 14 days 4/5 4/5 4/6 4/4 4/5 5/5 5/5 15th 5/6 6/4 5/5 5/5 4/5 6/4 6/4 16 days 5/5 5/5 5/4 5/5 5/4 6/3 5/5 17 days 6/4 5/6 5/5 5/4 5/5 4/5 6/4 18 days 6/5 6/5 5/4 5/5 5/5 4/5 4/4 19 days 6/5 5/5 4/5 5/5 4/4 6/4 4/6 20 days 5/4 4/6 5/6 4/5 5/5 4/4 5/5

(Number memorization skill evaluation score/figure memorization skill evaluation score)

Claims (6)

A brain health function improving composition comprising an extract of broccoli sprouts grown with lava seawater. The method according to claim 1,
The broccoli sprout extract is
Processing by applying pulse energy to broccoli sprouts (S100);
Preparing a sprout juice by squeezing the broccoli sprouts treated with pulse energy (S200);
Preparing a sprout fermented alcohol extract by mixing and reacting myrosinase with the sprout juice and extracting it with fermented alcohol (S300);
preparing a broccoli sprout concentrate by stirring the sprout fermented alcohol extract and concentrating the stirred broccoli sprout fermented alcohol extract under reduced pressure (S400);
Preparing the broccoli sprout extract by drying the broccoli sprout concentrate (S500); and
Brain health function improving agent composition which is prepared through; grinding the dried broccoli sprout extract into a powder (S600). The method according to claim 1 or 2,
The composition is a brain health function improving agent composition, characterized in that the memory improver. The method according to claim 1 or 2,
The composition is a brain health function improving agent composition, characterized in that the nerve cell death inhibitor. The method according to claim 1 or 2,
The composition is a brain health function improving composition, characterized in that the neuroinflammation inhibitor. The method according to claim 1 or 2,
The composition is a brain health function improving composition, characterized in that the neurotransmitter and synaptic plasticity recovery agent.

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