KR102556210B1 - Method for manufacturing health functional food containing broccoli stems - Google Patents

Abstract

The present invention relates to a method for manufacturing health functional food, including broccoli, complex enzyme powder, and natural medicine, which has an effect of improving comprehensive gastrointestinal health and improving chronic indigestion.
The health functional food of the present invention includes broccoli stems, various natural medicines and complex enzyme powders that help improve gastric health, so it can greatly help improve overall gastric health, and contains antioxidants derived from broccoli and natural medicines. Since the effect is significantly enhanced and the sensory evaluation is excellent, it can be usefully used in the field of health functional food.

Description

Method for manufacturing health functional food containing broccoli and enzymes {Method for manufacturing health functional food containing broccoli stems}

The present invention relates to a method for manufacturing health functional food, including broccoli, complex enzyme powder, and natural medicine, which has an effect of improving comprehensive gastrointestinal health and improving chronic indigestion.

Broccoli (Brassica oleracea L.) is a crop belonging to the Brassicaceae Brassica genus belonging to the Brassicaceae family. Broccoli is derived from a wild cabbage species of the Mediterranean coast, and is a vegetable mainly consumed in Western countries such as Europe and the United States. Globally, more than 75% is produced in China and India, and in Korea, broccoli cultivation and consumption have increased since 2000 along with an increase in preference for functional vegetables.

Broccoli contains 370mg of potassium per 100g, which lowers the risk of high blood pressure. It also contains folic acid, which lowers the risk of birth defects in pregnant women and prevents anemia, and chromium, which is beneficial for diabetics. On the other hand, the calories are 28 ㎉ per 100g, which is good for people on a diet to lose weight, and is known to have anticancer effects.

Enzyme is a protein that catalyzes all life activity reactions occurring in the body, and plays the most important role in digesting food and maintaining human life. The types of enzymes can be largely divided into food enzymes, metabolic enzymes, and digestive enzymes. Enzymes help our body break down the various foods we eat into nutrients and absorb them. On the other hand, since food enzymes can react with food raw material ingredients mixed together to digest the raw food composition when certain requirements are met, it is important to maintain a level that does not affect the quality of the product while maximizing the nutrient absorption effect of the raw food composition .

In a situation where interest in health functional foods derived from natural raw materials is gradually increasing, the present inventors tried to prepare a health functional food containing broccoli, complex enzyme powder, and natural drugs showing a complex gastrointestinal health improvement effect.

Patent Document (0001) Republic of Korea Patent Publication No. 10-2022-0051579

The present invention is effective in improving comprehensive gastrointestinal health and chronic indigestion by including a complex enzyme powder and a natural drug for improving gastric health in broccoli pulverized extract, which has significantly increased antioxidant effect by treating broccoli stem with a mixed extract of a specific raw material. The present invention was completed by confirming the excellence.

Accordingly, an object of the present invention is to provide a method for preparing a health functional food containing broccoli stem extract, complex enzyme powder, and natural medicine.

Another object of the present invention is to provide a health functional food containing broccoli stems prepared by the above production method.

In order to achieve the object of the present invention, the present invention provides a step of obtaining a pulverized product by pulverizing broccoli stems;

b) treating the pulverized product of step a) with complex extracts of creation, dermis, hawthorn, yeonmyeongcho, birch tree, and cheolseonryun;

c) steaming the pulverized material treated in step b) with steam for 2 to 2.5 hours;

d) adding complex enzyme powder to the pulverized material treated in step c); and

e) providing a health functional food manufacturing method comprising the step of drying the pulverized product treated in step d) at 4 to 10 ° C.

In one embodiment of the present invention, the weight ratio of the creation, dermis, hawthorn, Yeonmyeongcho, birch tree, and cheolseonryun extract may be 1: 1: 2: 3: 2: 1.

In one embodiment of the present invention, 10 parts by weight of the complex extract may be treated with respect to 100 parts by weight of the pulverized broccoli stem.

In one embodiment of the present invention, the step of preparing a ring by adding an excipient after step e) may be further included.

In addition, the present invention provides a health functional food prepared by the above method.

In the present invention, "health functional food" refers to a food prepared using raw materials or components having useful functions for the human body or nutrients that are easily deficient in daily meals, and refers to food that helps maintain the health of the human body, but is limited thereto. It does not mean that it includes all health foods in the usual sense.

In the present invention, the health functional food may be mixed with food additives in the form of tablets, capsules, pills, granules, liquids, powders, flakes, syrups, gels, jellies, bars, films, etc., and commercialized as health functional foods.

Food additives that can be mixed in the health functional food include, for example, flavoring agents, sweetening agents, flavoring agents, preservatives, natural carbohydrates, nutrients, vitamins, electrolytes, coloring agents, pectanes and salts thereof, alginic acid and salts thereof, organic acids , protective colloidal thickener, pH adjuster, stabilizer, preservative, glycerin, alcohol, etc., but is not limited thereto, and is limited as long as it can be added to food and can exhibit a beneficial effect on the health functional food composition of the present invention may be included without

In addition, the health functional food composition of the present invention, in addition to the active ingredient, improves intestinal function and enhances or reinforces the defecation promoting effect or enhances the convenience of intake or intake through the addition of similar activities such as blood pressure control activity, sugar It may further include any compound or natural extract whose safety has already been verified in the art and is known to have a corresponding activity. These compounds or extracts include compounds or extracts, pharmaceuticals listed in compendials such as pharmacopoeia of each country ("Korean Pharmacopoeia" in Korea) and health functional food codes of each country (in Korea, it is "standards and specifications for health functional foods", a notice of the Ministry of Food and Drug Safety). The laws of each country regulating the manufacture and sale of compounds or extracts and health functional foods that have been licensed under each country's laws ("Pharmaceutical Affairs Act" in Korea) governing the manufacture and sale of health functional foods ("Health Functional Foods Act" in Korea) ”), compounds or extracts whose functionality has been recognized are included.

The health functional food of the present invention includes broccoli stems, various natural medicines and complex enzyme powders that help improve gastric health, so it can greatly help improve overall gastric health, and contains antioxidants derived from broccoli and natural medicines. Since the effect is remarkably enhanced and the sensory evaluation is excellent, it can be usefully used in the health functional food field.

While developing a health functional food using broccoli stems, the inventor of the present invention completed the present invention by confirming that the effect of antioxidation and improvement of indigestion is excellent when a specific mixed extract is treated with broccoli stems and complex enzymes are added. did

Accordingly, the present invention comprises the steps of a) pulverizing broccoli stems to obtain a pulverized product;

b) treating the pulverized product of step a) with complex extracts of creation, dermis, hawthorn, yeonmyeongcho, birch tree, and cheolseonryun;

c) steaming the pulverized material treated in step b) with steam for 2 to 2.5 hours;

d) adding complex enzyme powder to the pulverized material treated in step c); and

e) providing a health functional food manufacturing method comprising the step of drying the pulverized product treated in step d) at 4 to 10 ° C.

In the present invention, the pulverized material means a product obtained by crushing solid particles, for example, broccoli stems into small pieces, and there is no limitation on the pulverizing means and the size of the pulverized particles.

In the present invention, the creation is a medicinal material made from the rhizome of shovel, a perennial herbaceous plant belonging to the Asteraceae family, and the taste of the medicine is bitter, very, and has warm medicinal properties. It is known to cure a cold, to strengthen the lower body, and to act as a stomach.

In the present invention, the dermis is a ripe fruit peel of Citrus unshiu Markovich or Citrus reticulata Blanco (Rutaceae Rutaceae). It is warm in nature, bitter in taste, has no poison, and is known to be effective for stomach upset, vomiting, and indigestion.

In the present invention, the hawthorn refers to the fruit of the hawthorn tree, and is known to be used to warm the stomach to promote digestion and lower the body.

In the present invention, the life-myeongcho is made by drying the entire aerial part (mainly leaves) of a plant of the same genus (屬) and grass, and is also referred to as a green flower. It refers to the outpost of Bangapul. It is a perennial plant with a height of 1~1.5m. It blooms in August~September, and the flower color is light purple. It is known to be used for gastric action, applied to snake or insect bites, jaundice, and fever with chills.

In the present invention, the birch tree uses young shoots as a medicine, and is also called heukhwa. It is known to stop pain and strengthen the digestive function of the stomach.

In the present invention, the cheolseonryun is a vine plant of the dicotyledonous plant Buttercup Orchidaceae, and uses the root as a medicine, strengthens the digestive function of the stomach, removes phlegm, relieves inflammation, and diarrhea or loose stools. It is known to be used in

In the present invention, the extract refers to a product such as a liquid component (extract solution) obtained by immersing an extraction target in a solvent and then extracting at room temperature or at elevated temperature for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. can The solvent is one or more solvents selected from the group consisting of water, C ₁ to C ₄ alcohol, hexane, and mixtures thereof, preferably an ethanol extract, and more preferably a 70% ethanol extract. Not limited.

In one embodiment of the present invention, the weight ratio of the creation, dermis, hawthorn, Yeonmyeongcho, birch tree, and cheolseonryun extract may be 1: 1: 2: 3: 2: 1.

In one embodiment of the present invention, 10 parts by weight of the complex extract may be treated with respect to 100 parts by weight of the pulverized broccoli stem.

Through the process of steaming the pulverized product treated in step b) in step c) with steam, the effect of enhancing the flavor of the broccoli stem and softening the fiber can be achieved, and the natural composite extract can permeate the broccoli stem as a whole. let it be The steaming time can be appropriately adjusted by a person skilled in the art, but may be preferably steamed for 2 to 2.5 hours.

In step d) of the present invention, complex enzyme powder may be added to the pulverized material after the steaming step c) is completed. Specifically, the complex enzyme powder is composed of protease, alpha-amylase, glucoamylase, lipase, alpha-galactosidase, cellulase, lactase, diastase, invertase, and dextrin, and 100 parts by weight of the pulverized product It may be included in 10 to 15 parts by weight compared to, and most specifically, it may be included in 12 parts by weight, but is not limited thereto.

In step e) of the present invention, the pulverized product mixed with the complex enzyme powder after step d) is completed may be dried at 4 to 10 ° C, more preferably at 7 ° C. Below the above temperature range, there is a risk of freezing the extract, and above the above temperature range, there is a risk that the components of the extract may deteriorate due to fermentation.

At this time, the drying time may be 7 to 9 hours drying, more preferably 8 hours drying. Drying may not be sufficient below the above time range, and it is difficult to expect significant drying compared to time above the above time range.

In the present invention, it was confirmed that antioxidant activity significantly increased when the extracts of the creation, dermis, hawthorn, Yeonmyeongcho, birch tree, and cheolseonryeon were treated with pulverized broccoli stems. In particular, the weight ratio of the extract was 1:1:2 In the case of :3:2:1, it was confirmed that there was a significantly increased antioxidant activity compared to other ratios (see Experimental Example 1).

In the present invention, the step of preparing a ring by adding an excipient after step e) may be further included. When prepared in pill form, it has the advantage of being easier to ingest than powder. The size of the ring is not limited and can be prepared by a conventional technique in the art, and an excipient such as flour may be added.

In another aspect, the present invention provides a health functional food containing broccoli stems prepared by the above method. The health functional food is excellent in antioxidant effect and indigestion improvement effect, and is also excellent in sensory quality, and therefore, a great deal of demand is expected in the corresponding field.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

<Example 1: Materials and Preparation>

Broccoli was purchased from Pyeongchang-gun, and after washing with water, only the stem was separated and pulverized, and Changjo, dermis, hawthorn, Yeonmyeongcho, birch tree, and Cheolseonryun were purchased from Gyeongdong Market.

The creation, dermis, hawthorn, yeonmyeongcho, birch tree, and cheolseonryun each take 300 g as a sample, add 10 times 70% ethanol of each sample, and repeat the process of stirring and extracting at room temperature and 80 rpm for 12 hours twice. did The extracts obtained twice were combined, filtered first (Whatman NO. 1), concentrated under reduced pressure with a rotary evaporator on a water bath at 40 ° C, and filtered second (GelmanScience PTFE membrane filter, 0.45 μm) to obtain samples.

Each fraction was sterilized with a 0.45 μm membrane filter (Adantec MFS, Inc., CA) and stored in a refrigerator at 2-4 ° C. On the other hand, each extract was mixed in a weight ratio as shown in Table 1 and dissolved in water to adjust to 1 g/ml.

Creation dermis sansa life support plant birch tree iron wire sample 1 One One One One One One sample 2 One One 2 3 2 One sample 3 One One 3 5 3 One sample 4 One One 3 2 One One sample 5 One One 2 2 2 One

The complex enzyme powder was purchased and used on the market, and an automatic ventilation machine (KSJ NO. 4) was used to manufacture the pill.

<Example 2: Preparation of health functional food containing broccoli stems and extracts derived from natural products>

100 g of samples 1 to 5 of Example 1 were spray-sprayed on 1000 g of pulverized broccoli stems, mixed evenly, and steamed for 2 hours with steam.

Then, after drying in a shady place at 7 ° C. for 8 hours, large solids were removed using a sieve, and an appropriate amount of wheat flour was added as an excipient to prepare a round-shaped health functional food with automatic ventilation.

<Example 3: Manufacturing health functional food containing broccoli stem, complex enzyme powder and extract derived from natural products>

100 g of Sample 2 of Example 1 was spray-sprayed on 1000 g of pulverized broccoli stems, mixed evenly, and then steamed with steam for 2 hours.

Then, 120 g of complex enzyme powder was added and dried for 8 hours in a shaded place at 7°C. Detailed components of the complex enzyme powder are as described above.

Then, large solids were removed using a sieve, and an appropriate amount of wheat flour was added as an excipient to prepare a round-shaped health functional food with automatic ventilation.

<Experimental Example 1: Confirmation of antioxidant activity enhancement effect of broccoli stems by mixed extract>

In order to confirm the enhancement of the antioxidant effect of broccoli stems by the mixed extract treatment, the following experiments were conducted by preparing samples prepared by the method of Example 2 using the extract samples 1 to 5 and samples not mixing the extracts.

DPPH radical scavenging activity

In order to confirm DPPH (2,2-diphenyl-2-picryl-hydrazyl) radical scavenging activity, 900 μl of DPPH solution (150 μM) and 100 μl of each sample were mixed and stirred, and then reacted for 30 minutes in the dark. Absorbance was measured in nm. The DPPH radical scavenging activity was averaged by repeating each experiment three times, and then calculated by Equation 1 below to measure the concentration (IC ₅₀ ) of the sample required for 50% DPPH radical scavenging by the sample and shown in the table.

[Equation 1]

DPPH radical scavenging activity (%) = (A-B)/A x 100

A: Absorbance of DPPH with methanol added instead of sample

B: Absorbance of DPPH with sample added

sample DPPH radical scavenging activity
(IC ₅₀ μg/ml) broccoli >500 Broccoli + sample 1 427.3 Broccoli + Sample 2 191.7 Broccoli + Sample 3 301.2 Broccoli + Sample 4 376.5 Broccoli + Sample 5 417.2

As shown in Table 2 above, it can be seen that the DPPH radical scavenging activity increases when the extracts of creation, dermis, hawthorn, Yeonmyeongcho, birch tree, and cheolseonryun are added compared to broccoli alone. In particular, creation, dermis, hawthorn, In the case of sample 2, in which vinegar, birch, and iron wire were mixed at a weight ratio of 1:1:2:3:2:1, it was confirmed that there was a significant DPPH radical scavenging activity compared to the addition of other samples.

ABTS radical scavenging activity

To confirm the radical scavenging activity of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)), 7 mM ABTS and 2.45 mM potassium persulphate were added to distilled water and reacted at room temperature for 16 hours. After generating ABTS cations, it was prepared by diluting so that the value of absorbance at 734 nm was 0.7 or less. Then, 900 μl of the ABTS radical solution and 100 μl of each sample were mixed and stirred. After reacting this mixed sample at 30 ° C. for 6 minutes, the absorbance value was measured. The ABTS radical scavenging ability was averaged by repeating each experiment three times, and then the degree of decrease in absorbance for the control group was calculated by Equation 2 below. The concentration (IC ₅₀ ) of the sample required to scavenge 50% of ABTS radicals was measured.

[Equation 2]

ABTS radical scavenging activity (%) = (A-B)/A x100

A: Absorbance of ABTS with methanol added instead of sample

B: Absorbance of ABTS to which the sample was added

sample ABTS radical scavenging activity
(IC ₅₀ μg/ml) broccoli 450.9 Broccoli + Sample 1 251.8 Broccoli + Sample 2 111.3 Broccoli + Sample 3 349.1 Broccoli + Sample 4 258.1 Broccoli + Sample 5 227.8

As shown in Table 3, it can be seen that the ABTS radical scavenging activity increased when the extracts of creation, dermis, hawthorn, yeonmyeongcho, birch tree, and cheolseonryun were added compared to broccoli alone. In particular, in the case of sample 2, other samples were added It was confirmed that there is a significant ABTS radical scavenging activity compared to.

<Experimental Example 2: Improvement of digestive function and confirmation of preference>

Experimental Example 2-1. Confirmation of digestive function enhancement effect

A sample was prepared by the method of Example 3 by adding a complex enzyme powder based on the components of Sample 2, which were confirmed to have the highest antioxidant capacity.

At this time, the rest of the process was prepared in the same way as in Example 3, but the sample containing no complex enzyme powder was prepared in Comparative Example 1, the sample containing 90 g of complex enzyme powder in Comparative Example 2, and the sample containing 160 g in Comparative Example 3, including 200 g One was set as Comparative Example 4.

In the experiment, a sensory test group consisting of a total of 20 people, 10 men and women in their 20s to 50s, was instructed to eat raw food on an empty stomach at least twice a day, morning and evening, for 3 days, and then scored a total of 5 points for the questions below. items were displayed. The higher the satisfaction, the higher the score. For example, 0 points were given if digestion was not at all, including the feeling of bloating, and 5 points were given if digestion was very good. The average of each item is shown in Table 4 below.

Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 score average 4.8 2.8 3.4 4.1 2.9

As a result, Example 3 received the highest score, and Comparative Example 1, which did not contain at all, received the lowest score. In the case of Comparative Example 4 containing an excessive amount (200 g) of complex enzyme powder, contrary to expectations, the digestive function enhancement effect was rather poor.

Experimental Example 2-2. check the symbol

Since the present invention is a health functional food, preference for intake is also a very important factor, so sensory evaluation was conducted for taste, aroma, and overall preference. The evaluation was averaged on a 5-point scale by grading very bad (1 point), slightly bad (2 points), average (3 points), moderately good (4 points), and very good (5 points).

Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 taste 4.2 3.5 4.0 4.2 3.9 incense 4.4 3.4 3.8 4.2 3.6 Overall Preference 4.6 3.4 4.1 4.3 3.7

As shown in Table 5, Example 3 received the highest score overall, and in the case of the complex enzyme powder, it has a unique taste, so it is included in an excessive amount (Comparative Example 4) or not (Comparative Example 1). It was confirmed that the degree of preference was generally high when it was included. The various embodiments described above may be embodied in other specific forms without departing from their technical ideas and essential characteristics. Accordingly, the above detailed description should not be construed as limiting in all respects and should be considered illustrative. The scope of the various embodiments should be determined by a reasonable interpretation of the appended claims, and all changes within the equivalent scope of the various embodiments are included in the scope of the various embodiments. In addition, claims that do not have an explicit citation relationship in the claims may be combined to form an embodiment or may be included as new claims by amendment after filing.

Claims (7)

a) crushing broccoli stems to obtain a pulverized product;
b) treating the pulverized product of step a) with a complex extract mixed with creation, dermis, hawthorn, yeonmyeongcho, birch tree, and cheolseonryun in a weight ratio of 1: 1: 2: 3: 2: 1;
c) steaming the pulverized material treated in step b) with steam for 2 to 2.5 hours;
d) adding complex enzyme powder to the pulverized material treated in step c); and
e) in the health functional food manufacturing method comprising the step of drying the pulverized product treated in step d) at 4 to 10 ° C. for 7 to 9 hours,
The complex extract contains 5 to 20 parts by weight based on 100 parts by weight of the pulverized broccoli stem, and the complex enzyme powder is included in 12 parts by weight based on 100 parts by weight of the pulverized broccoli stem,
The complex enzyme powder comprises at least one selected from the group consisting of protease, alpha-amylase, glucoamylase, lipase, alpha-galactosidase, cellulase, lactase, diastase and invertase,
Method for manufacturing health functional food. delete delete delete A health functional food containing broccoli stems prepared by the method of claim 1. The health functional food containing broccoli stems prepared by the method of claim 1 is manufactured in the form of any one of pills, powders, pellets, and granules. The health functional food containing broccoli stems prepared by the method of claim 1 has an antioxidant or digestive enhancing effect.