KR102155187B1 - Composition for functional food having atopic dermatitis treatment property and antioxidant property and method for manufacturing the same - Google Patents

Abstract

The present invention is a health functional food having anti-oxidative and atopic dermatitis treatment ability as an active ingredient comprising a complex mixture containing Nandamban, Jajuk salt, Garlic extract, Malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract It relates to the composition. Through this, the present invention provides a composition for a health functional food that does not cause toxicity and has atopic dermatitis treatment and antioxidant activity.

Description

A composition for a health functional food having atopic dermatitis treatment and antioxidant activity, and a manufacturing method thereof {COMPOSITION FOR FUNCTIONAL FOOD HAVING ATOPIC DERMATITIS TREATMENT PROPERTY AND ANTIOXIDANT PROPERTY AND METHOD FOR MANUFACTURING THE SAME}

The present invention relates to a composition for a health functional food having anti-oxidative ability and atopic dermatitis treatment ability containing Nandamban and a method for producing the same.

The skin is the largest tissue in the human body and performs various functions such as body temperature regulation, secretion, excretion, and absorption. It is located on the outermost part of the body and has the function of protecting the human body physically, chemically, and biologically from the outside. In the skin that is constantly exposed to external environmental stress, skin elasticity decreases and moisture loss occurs due to peroxidation of cell membrane lipids, weakening of immunity, inflammatory reaction, and disruption of extracellular matrix, resulting in skin aging. . In order to prevent aging of the skin and restore the function of damaged skin, ingesting vitamins, minerals, antioxidants, etc. or directly applying it to the skin is very important in maintaining the normal condition of the skin.

Atopic dermatitis is an inflammatory skin disease that occurs mainly in infancy or childhood and is chronically accompanied by redness, edema, pruritus, epidermal peeling, and dry skin. The cause of the disease is estimated from environmental factors, genetic predisposition, and immunological responses. In demographic studies, atopic dermatitis is reported to occur in 0.5 to 1% of the population of industrialized countries (5 to 10% for infants), and the incidence of residents living in industrial and dry areas is high.

Factors involved in atopic dermatitis include inflammatory mediator cytokines such as IgE, TNF-α and IL-1β, histamine, prostaglandin, and lipid inflammatory mediators such as leukotriene, COX. Inflammatory enzymes such as -2 (cyclooxygenase-2), LO (lipoxygenase), and iNOS (inducible nitric oxide synthase). Immune cells involved in atopic dermatitis include mast cells, macrophages, and various lymphocytes.

Although modern medicine has developed, there is no cure medical technology for atopic skin disease, which is on the rise, and treatments focusing on symptom relief (topical steroids, local immunomodulators, systemic steroids, systemic immunosuppressants, antihistamines, interferon gamma, etc.) are available. It is mainly done. Therefore, patients are expecting a lot of natural substances for fundamental treatment with no side effects of synthetic drugs and no recurrence of disease.

On the other hand, gallbladder is a kind of sulfate mineral composed of copper sulfate, a natural mineral containing CuSO ₄ ·5H ₂ O as a main component, and is a blue crystal in which the main component and other trace minerals are mixed. Gallbladder is a mineral herbal medicine, so there is a risk of toxicity, so there is a limit to its practical use.

Therefore, there is an increasing public demand for a composition for a health functional food having excellent safety and efficacy because it does not induce cytotoxicity, and has excellent atopic dermatitis treatment and antioxidant activity.

One object of the present invention is to provide a composition for a health functional food that does not cause toxicity and has atopic dermatitis treatment and antioxidant activity.

Another object of the present invention is to achieve the safety and effectiveness of the complex mixture containing Nandamban to an excellent degree, and without causing toxicity, but without causing atopic dermatitis treatment and antioxidant activity of a composition for health functional foods It is to provide a useful manufacturing method.

One embodiment of the present invention has a therapeutic and antioxidant activity for atopic dermatitis using a complex mixture containing Nandamban, Jajuk salt, Garlic extract, Malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract as an active ingredient. It relates to a composition for health functional food.

The Nandamban may be obtained by mixing heated and dehydrated gallbladder powder and egg white of eggs in a weight ratio of 1:0.2 to 1:1.

Another embodiment of the present invention relates to a method for preparing a composition for a health functional food having the above-described atopic dermatitis treatment ability and antioxidant ability.

The method for preparing a composition for a health functional food having atopic dermatitis treatment and antioxidant activity includes preparing Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract, respectively; And preparing a complex mixture by mixing the respective Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract; Includes.

In the preparing step, in the preparation of the Nandamban, the gallbladder is heated at 100°C to 150°C, dehydrated so that the moisture content is 0.001% to 5%, and then completely cooled at room temperature, and then powdered to prepare the damban powder. It may be obtained by mixing the egg white of the egg with the gallbladder powder in a weight ratio of 1:0.2 to 1:1.

The complex mixture is Nandamban 0.1% to 50% by weight, Jajuk salt 1% to 90% by weight, 1% to 20% by weight of garlic extract, 0.5% to 40% by weight of malt extract, from 0.5% by weight of gold and silver extract It may contain 5% by weight, 0.5% to 5% by weight of Pogongyeong extract, 0.5% to 5% by weight of Yugeunpi extract, and 0.5% to 5% by weight of Baekgangsam extract.

The present invention provides a composition for a health functional food having an anti-oxidative ability and atopic dermatitis treatment ability without causing toxicity, and the above health that can achieve the safety and effectiveness of a complex mixture containing Nandamban to an excellent degree. It provides an industrially useful manufacturing method of a composition for functional food.

1 shows the results of the antioxidant activity evaluation experiment of Experimental Example 1.
2 shows the result of measuring the inhibition of enzyme activity related to COX-2 of Experimental Example 2.
3 shows the results of COX-2-related immunocytochemical staining of Experimental Example 3.
Figure 4 shows the results of iNOS-related immunocytochemical staining of Experimental Example 4.
5 shows the results of observation of skin tissue lesions in the animal model of atopic dermatitis of Experimental Example 5.
6 shows the results of histopathological examination in the animal model of atopic dermatitis of Experimental Example 5.

The term “extract” used in the present specification is obtained by a general extraction method from each target raw material, and is not limited according to the state (pahse) of the material, and may be, for example, a liquid phase, a solid phase, or a mixed phase.

One embodiment of the present invention has a therapeutic and antioxidant activity for atopic dermatitis using a complex mixture containing Nandamban, Jajuk salt, Garlic extract, Malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract as an active ingredient. It relates to a composition for health functional food. Through this, the present invention provides a composition for a health functional food having an anti-oxidative ability and atopic dermatitis treatment ability without causing toxicity.

In addition, the composition for health functional food of the present invention inhibits the activity and expression of the inflammation-inducing enzyme COX-2 (cyclooxygenase-2), inhibits degranulation of mast cells, inhibits histamine release, and has inflammatory properties. It suppresses the secretion of cytokine and can realize excellent antioxidant activity.

In particular, the composition for health functional foods of the present invention is effective in the overall synergistic action of Nandamban, Jajuk salt, garlic extract, malt extract, gold silver flower extract, pogongyeong extract, Yugeunpi extract and Baekgangjam extract contained in the complex mixture used as an active ingredient. As a result, compared to the case where each component is used in the same amount, it is possible to achieve remarkably improved inhibition of the activity of cyclooxygenase-2 (COX-2). Through this, the composition for health functional food of the present invention not only realizes excellent anti-inflammatory effect, but also prevents the occurrence of atopic dermatitis promoted by inflammatory proteins, and implements atopic dermatitis improvement ability and atopic dermatitis treatment ability.

Among the total complex mixtures, Nandamban is a mixture of egg white and gallbladder from eggs, and the heated and dehydrated gallbladder (a natural mineral containing CuSO ₄ 5H ₂ O as a main component) is powdered, and then the egg white is mixed and reacted. To manufacture. In the Nandamban prepared in this way, the toxicity peculiar to the gallbladder is neutralized by egg white, so that the toxicity is reduced or eliminated, and the drug properties are enhanced.

The Nandamban may be obtained by mixing the egg white of the egg with respect to the gallbladder powder in a weight ratio of 1:0.2 to 1:1, specifically 1:0.2 to 1:0.9, and more specifically 1:0.3 to 1:0.7. Within the above range, the Nandamban may not cause toxicity, and may implement more excellent anti-inflammatory activity, activity inhibiting activity of COX-2, and treatment of atopic dermatitis.

The content of Nandamban in the total composite mixture may be 0.1 wt% to 50 wt%, specifically 0.5 wt% to 30 wt%, and more specifically 1 wt% to 20 wt%. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent anti-inflammatory activity, activity inhibition activity of COX-2, and atopic dermatitis treatment capacity.

Among the total complex mixtures, bamboo salt is prepared by repeatedly melting by hot treatment by adding natural sea salt or bamboo salt in a bamboo barrel, and if it is a general bamboo salt, it is not particularly limited. The so-prepared jacquard salt not only strengthens the stomach and has excellent detoxification effects, but also increases white blood cells to enhance sterilization, is excellent for blood purification, anti-inflammatory, and antipyretic, purifies the air, removes odor, and promotes appetite. Has the efficacy of. In addition, unlike sea salt, heavy metals are completely removed, and various minerals are more abundant, and it is characterized by showing alkalinity.

The bamboo salt used for the bamboo salt may be commercially available, or may be directly prepared and used. Specifically, for bamboo salt used in the manufacture of bamboo salt, the western coastal sea salt is put in king bamboo, capped with ocher, then put in a metal container, and then, after burning with pine, the remaining salt pillars are crushed. As in the first step, it is again put in a new bamboo tree, sealed with ocher, and burned with pine in a steel container 8 times.The last ninth time is manufactured by the ninth molten bamboo salt manufacturing method in which the salt is dissolved by adding rosin to increase the heat. However, it is not limited thereto.

Specifically, the jajuk salt may be 9-time jajuk salt obtained by crushing Korean sea salt into the king bamboo of Jirisan, and repeating the process of burning it with pine firewood 8 times, and the ninth, melted with pine resin over high heat of 1,400°C. These 9-time jajukitis achieve an effective improvement rate of 91.95% for gastrointestinal patients such as gastritis and gastric ulcers, and there is no toxicity or damage to the human body of the stomach or intestine. In addition, unlike conventional salts and sea salts, which are acidic, they are alkaline with a pH of 8 to 13, have an effect of neutralizing an acidified diet, and can implement an effect of suppressing the production of substances that cause inflammation.

The content of jajuk salt in the total complex mixture may be 1% by weight to 90% by weight, specifically 5% by weight to 87% by weight, and more specifically 20% by weight to 85% by weight. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, can implement an effect of reducing liver toxicity, and can implement more excellent anti-inflammatory properties and atopic dermatitis treatment ability.

The garlic extract of the total complex mixture is not particularly limited as long as it is an extract obtained by removing the skin of garlic fruit. The garlic extract contains a high content of components such as vitamin B1, vitamin B2, vitamin C, glutamic acid, calcium, iron, phosphorus, zinc, selenium, and allicin derived from garlic growth, anti-cancer, anti-inflammatory and It realizes the immunity enhancement effect and can implement a strong antioxidant effect, so it has excellent health supplementary efficacy.

In addition, the garlic extract is excellent in sterilizing, insecticidal, and vicious growth, and is effective against various diseases (herbal herb gangmok), no harm to the human body even if taken for a long time (Sanong herbal), sterilization, intestinal tract, respectively. It can improve, improve whooping cough, improve pulmonary tuberculosis, and exert effects such as tonic.

The garlic extract is not particularly limited, but may be an extract extracted by heating at 80°C to 100°C after adding 2 to 6 folds of water or 10 v/v% to 60 v/v% hydrated ethanol for garlic growth. have. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability.

The solid content of the garlic extract may be 2% to 15% by weight of the garlic extract. In this case, the garlic extract can provide an excellent flavor for application as a composition for health functional foods while providing anti-inflammatory and antioxidant active ingredients in a high content.

The content of the garlic extract in the total complex mixture may be 1% to 20% by weight, specifically 5% to 20% by weight, and more specifically 10% to 20% by weight. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability.

The malt extract of the whole complex mixture is not particularly limited as long as it is roasted sprouts of the rice family, Hordeum vulgare Linn, and the malt extract is vitamin B derived from malt, iron, folic acid, lecithin and By providing ingredients such as potassium in a high content, it can help digestion by protecting the liver and promoting bile production and provide antioxidant effects, so it has excellent health supplemental efficacy.

The malt extract is not particularly limited, but may be an extract extracted by heating at 80° C. to 100° C. after adding 2 to 10 times water or 10 v/v% to 60 v/v% hydrated ethanol. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability.

The solid content of the malt extract may be 2% by weight to 10% by weight. In this case, the malt extract may provide an anti-inflammatory and antioxidant active ingredient in a high content, and provide excellent flavor for application as a composition for health functional food.

The content of the malt extract in the total complex mixture may be 0.5% to 40% by weight, specifically 1% to 40% by weight, more specifically 4% to 30% by weight. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and while implementing more excellent antioxidant, anti-inflammatory and atopic dermatitis treatment capabilities, it is possible to further enhance the effect of reducing human toxicity.

In the total complex mixture, the extract of gold and silver is not particularly limited as long as it is an extract of dried honeysuckle (Lonicerae Japonica Thunberg). Specifically, the gold and silver flower extract may be an extract of a gold and silver flower belonging to the honeysuckle. The gold and silver flower extract contains flavonoid components such as luteolin, apigenin, and quercetin in a high content, thereby implementing anticancer, anti-inflammatory and immunity enhancing effects, and preventing food poisoning, preventing gingivitis, etc. As it can impart an effect, health supplemental efficacy is more excellent.

The gold and silver flower extract is not particularly limited, but may be an extract extracted by heating at 80° C. to 100° C. after adding 2 to 6 folds of water or 10 v/v% to 60 v/v% hydrated ethanol. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability.

The solid content of the gold and silver extract may be 1% to 8% by weight. In this case, the extract can provide an excellent flavor to be applied as a composition for health functional food while providing anti-inflammatory and antioxidant active ingredients in a high content.

In the total complex mixture, the extract may be included in an amount of 0.5% to 5% by weight. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and while implementing more excellent antioxidant, anti-inflammatory and atopic dermatitis treatment capabilities, it is possible to further enhance the effect of reducing human toxicity.

Among the total complex mixtures, pogongyoung extracts were dried outposts of similar plants such as dandelion (Taraxacum platycarpum H. Dahlstedt), hairy dandelion (Taraxacum mongolicum Handel-Mazzetti), white dandelion (Taraxacum coreanum Nakai) and western dandelion (Taraxacum officinale Weber). If it is an extract, it is not particularly limited. The pogongyoung extract contains a high content of ingredients such as taraxacin, taraxarol, asparagin, etc., and implements anticancer, anti-inflammatory and immunity enhancing effects, and antipyretic, anti-inflammatory, diuretic, and dry stomach , Antipyretic detoxification, antibacterial effect can be imparted, health supplemental efficacy is more excellent.

The pogongyeong extract is not particularly limited, but may be an extract extracted by heating at 80° C. to 100° C. after adding 2 to 6 times water or 10 v/v% to 60 v/v% hydrated ethanol. Within the above range, the composition for health functional food of the present invention does not cause toxicity, and can implement more excellent antimicrobial, antioxidant, anti-inflammatory and atopic dermatitis treatment capabilities.

The solid content of the pogongyeong extract may be 1% to 8% by weight. In this case, the pogongyeong extract can provide an excellent flavor for application as a composition for health functional foods while providing antibacterial, anti-inflammatory and antioxidant active ingredients in a high content.

Pogongyeong extract of the total complex mixture may be included in 0.5% by weight to 5% by weight. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and while implementing more excellent antioxidant, anti-inflammatory and atopic dermatitis treatment capabilities, it is possible to further enhance the effect of reducing human toxicity.

Among all the complex mixtures, the extract of Yugeunpi refers to the extract of dried bark of the root of Ulmus davidiana var. japonica. The Yugeunpi extract contains a high content of luteol and phytosterol components, and can implement effects on cell protection, anti-inflammatory, asthma and immunity enhancement, and thus has more excellent health supplemental efficacy.

The Yugeunpi extract is not particularly limited, but may be an extract extracted by heating at 80° C. to 100° C. after adding 4 to 10 times water or 10 v/v% to 60 v/v% hydrated ethanol. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent cell protection, antioxidant, anti-inflammatory and atopic dermatitis treatment.

The solid content of the Yugeunpi extract may be 2% by weight to 10% by weight. In this case, the Yugeunpi extract can provide excellent flavor for application as a composition for health functional foods, while providing a high content of cell-protective, anti-inflammatory and antioxidant active ingredients.

The Yugeunpi extract may be included in 0.5% to 5% by weight of the total complex mixture. Within the above range, the composition for health functional food of the present invention does not cause toxicity, and while implementing more excellent cell protection, antioxidant, anti-inflammatory and atopic dermatitis treatment, can further enhance the effect of reducing human toxicity. have.

Among the complex mixtures, the extract of white ganglia refers to the extract of the body of the silkworm Bombyx mori (Batrytis bassiana) killed by leukemia caused by the infection of the white ganglia (Beauveria bassiana (Bals.) Vuill.). The Baekgangjam extract is mainly used in oriental medicine for circulatory diseases such as stroke, and can be mixed with other herbal medicines to give an effect to headache, epilepsy, sore throat, pruritus, etc., and thus, has more excellent health supplemental efficacy.

The extract of Baek chinensis is not particularly limited, but may be an extract extracted by heating at 80° C. to 100° C. after adding 2 to 6 folds of water or 10 v/v% to 60 v/v% hydrated ethanol. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, reduces itching and pain triggered by atopic dermatitis, and realizes more excellent antioxidant activity, anti-inflammatory activity, and therapeutic ability for atopic dermatitis. have.

The solid content of the extract of Baekgang champa may be 0.5% to 10% by weight. In this case, Baekgangjam extract may provide an itching and pain reducing active ingredient, anti-inflammatory and antioxidant active ingredients in a high content, while providing excellent flavor for application as a composition for health functional foods.

The Baek glacius extract may be included in an amount of 0.5% to 5% by weight of the total complex mixture. Within the above range, the composition for a health functional food of the present invention does not cause toxicity, and can implement more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability.

Another embodiment of the present invention relates to a method for preparing a composition for a health functional food having the above-described atopic dermatitis treatment ability and antioxidant ability. Through this, the present invention can achieve the safety and effectiveness of the complex mixture containing Nandamban to an excellent degree, and without causing toxicity, the preparation of a composition for a health functional food having atopic dermatitis treatment and antioxidant activity It provides a standardized, industrially useful manufacturing method.

The method for preparing a composition for a health functional food having atopic dermatitis treatment and antioxidant activity includes preparing Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract, respectively; And preparing a complex mixture by mixing the respective Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract; Includes.

In the preparing step, the preparation of Nandamban is specifically by heating the damban (a natural mineral containing CuSO ₄ ·5H ₂ O) at 100°C to 150°C, dehydrating so that the moisture content becomes 0.001% to 5%, After complete cooling at room temperature, it may be powdered to prepare a gallbladder powder, and the egg white of an egg may be mixed with the damban powder in a weight ratio of 1:0.2 to 1:1. Through the method of preparing the Nandamban, the toxicity of the Nandamban is eliminated and the drug properties are enhanced, so that it has an advantageous effect to be applied as a pharmaceutical or food composition for the treatment of atopic dermatitis.

The heating and dehydration of the damban may be performed by putting it in a cauldron and baking it while maintaining a temperature range of 100°C to 150°C over a gas fire, wood fire or charcoal fire. During heating, while watching the color change at intervals of 3 to 5 hours, heat is uniformly applied by mixing the top and bottom to further enhance the efficacy and uniformity of the gallbladder. The heating time may be adjusted according to the amount of gallbladder heated at one time, but may be heated for 5 to 24 hours based on, for example, 600 g. The heating and dehydration of the gallbladder may be performed until the color of the entire amount of the gallbladder turns gray to become anhydrous or the moisture content is 0% to 5%.

The heated and dehydrated gallban may be cooled at room temperature until heat is completely removed. It is desirable to finely grind and powder the wall plate cooled at room temperature, and store the powdered wall plate in a plastic bag or airtight container to prevent moisture from being absorbed.

For egg white, the remaining egg is used after removing the yolk from the egg. As for the egg, it is preferable to use a native egg, and more preferably, an Ogol-based egg may be used.

The method of mixing the gallbladder powder and the egg white is not particularly limited, but mixing with a tool that has little reactivity such as a wooden spatula can uniformly produce the efficacy of the finally produced Nandamban. In addition, the mixing is preferably carried out using a container in which a chemical reaction does not occur, for example, an earthenware, ceramic, or elvan stone container. In the mixing process, high heat may be generated due to the heat of reaction. When mixing the gallbladder powder and the egg white, the egg white can be added in small portions according to the progress of the reaction, through which the reactivity is adjusted to further improve the effect of neutralizing the toxicity of the gallbladder powder, and the miscibility of the Nandamban by the reaction heat This can be further improved. After sufficiently mixing the mixture of gall powder and egg white, it is preferable to cool the heat until the reaction heat generated during the reaction completely disappears.

The mixture of the gallbladder powder and the egg white is reacted by mixing the egg white of the egg with respect to the gallbladder powder in a weight ratio of 1:0.2 to 1:1, specifically 1:0.2 to 1:0.9, and more specifically 1:0.3 to 1:0.7 I can make it. Within the above range, the Nandamban may not cause toxicity, and may implement more excellent anti-inflammatory activity, activity inhibiting activity of COX-2, and treatment of atopic dermatitis.

In the preparing step, the preparation of the Nandamban may further include powdering after the reaction heat is completely removed. The powdering method is not particularly limited, but may be, for example, grinding and finely powdering a solid lumpy Nandamban. By powdering the Nandamban in this way, it can be effectively used in pharmaceutical or food preparations, and the reactive area of the Nandamban can be increased, thereby maximizing the therapeutic activity of the disease.

In the above preparation step, the preparation of birch salt may be to chop Korean sea salt into the king bamboo of Jirisan, repeat the process of burning it with pine firewood 8 times, and melt the ninth with pine resin at high heat of 1,400°C or higher. Through the preparation method of such salt, the salt has no toxic or damaging effect on the human body, has an effect of neutralizing an acidified diet, and further enhances the effect of inhibiting the production of substances that cause inflammation, so that atopic dermatitis It has an advantageous effect to be applied as a pharmaceutical or food composition for treatment.

In the above preparation step, the preparation steps of each of the garlic extract, malt extract, gold silver flower extract, pogongyeong extract, yugeunpi extract and Baekgangjam extract are not particularly limited.

Specifically, it may be an extract obtained by heating at 80°C to 100°C after adding 2 to 10 times water or 10 v/v% to 60 v/v% hydrated ethanol to each material to be extracted. Through such a preparation method of each extract, each extract does not cause toxicity, reduces itching and pain triggered by atopic dermatitis, and realizes more excellent antioxidant activity, anti-inflammatory activity, and atopic dermatitis treatment ability. have.

The step of preparing the complex mixture includes mixing each of Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract prepared by the above-described method.

The complex mixture is Nandamban 0.1% to 50% by weight, Jajuk salt 1% to 90% by weight, 1% to 20% by weight of garlic extract, 0.5% to 40% by weight of malt extract, from 0.5% by weight of gold and silver extract It may contain 5% by weight, 0.5% to 5% by weight of Pogongyeong extract, 0.5% to 5% by weight of Yugeunpi extract, and 0.5% to 5% by weight of Baekgangsam extract.

Details of each component included in the complex mixture are as described above.

The method for preparing a composition for a health functional food having atopic dermatitis treatment ability and antioxidant ability of the present invention comprises the steps of adding and applying the complex mixture prepared as described above to food; It may further include.

The food is not particularly limited as long as it can be used as a composition for a health functional food and does not impair the efficacy of the complex mixture of the present invention. The food is a health functional food, for example, beverages such as extracts and drinks; Confectionery products such as confectionery, bread, and rice cakes; It may be a kind that can be used as such.

Another embodiment of the present invention relates to a composition for the treatment of atopic dermatitis comprising a complex mixture containing Nandamban, Jajuk salt, garlic extract, malt extract, gold silver flower extract, pogongyeong extract, Yugeunpi extract and Baekgangjam extract as an active ingredient. . Through this, the present invention can provide a composition for treating atopic dermatitis, which does not cause toxicity, and has atopic dermatitis improving ability and antioxidant activity.

Details of the complex mixture included as an active ingredient in the composition for treating atopic dermatitis are the same as described above.

The composition for treating atopic dermatitis may include a complex mixture and a pharmaceutically acceptable carrier. The kind of the pharmaceutically acceptable carrier is not particularly limited as long as it does not impair the efficacy of the complex mixture of the present invention.

The composition for treating atopic dermatitis may be formulated and used as pharmaceuticals such as tablets, powders, and capsules.

Example

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this is presented as a preferred example of the present invention and cannot be construed as limiting the present invention in any sense.

Contents not described in the present specification can be sufficiently technically inferred by those skilled in the art, and thus description thereof will be omitted.

Manufacturing Example 1

600g of gallbladder was heated at 140° C. for 4 hours to completely dehydrate, cooled at room temperature, and powdered with a mortar. The egg white prepared by separating the egg yolk into the thus-prepared gallbladder powder was mixed so that the weight ratio of the gallbladder powder: egg white was 1:0.9, the reaction heat was cooled, and then powdered with a mortar to prepare Nandamban powder.

Manufacturing Example 2

After putting sea salt of the west coast in king bamboo, capping it with loess, then putting it in a bamboo salt furnace (iron container), burning it at 800℃ over a pine wood fire, crushing the remaining salt pillars, and putting it in a new king bamboo tree to make loess. After sealing and burning pine trees in a bamboo salt furnace (metal container) eight times, the last ninth time, add rosin and raise the temperature to 1400℃, cool the molten salt column at room temperature for 12 hours, pulverize, and cut 9 times. Bamboo salt was prepared.

Manufacturing Example 3

The garlic extract was obtained by hot water extraction at 100° C. for 2 hours by adding 4 times of drinking water to the growth of garlic. The content of solids in the garlic extract prepared as described above was 7.8% by weight.

Manufacturing Example 4

Drinking water of 4 folds was added to the malt, followed by hot water extraction at 100° C. for 2 hours to obtain a garlic extract. The content of solids in the malt extract prepared as described above was 4.5% by weight.

Manufacturing Example 5

Drinking water of 4 times was added to the dried gold and silver coins, and hot water extraction was performed at 100° C. for 4 hours to obtain a gold and silver extract. The solid content in the gold and silver extract prepared as described above was 4.5% by weight.

Manufacturing Example 6

Pogongyoung extract was obtained by hot water extraction at 100°C for 4 hours by adding 4 times of drinking water to the dry matter. The solid content of the pogongyeong extract prepared as described above was 3.2% by weight.

Manufacturing Example 7

10 times of drinking water was added to the dried yugeun-pi, and hot water extraction was performed at 100° C. for 4 hours to obtain a yugeun-pi extract. The content of solids in the Yugeunpi extract prepared as described above was 6.7% by weight.

Manufacturing Example 8

Four folds of drinking water was added to Baekgangjam and extracted with hot water at 100°C for 4 hours to obtain a Yugeunpi extract. The content of the solid content in the extract of Baekgangjam prepared as described above was 4.8% by weight.

Example 1

Nandamban prepared in Preparation Examples 1 to 8, 5% by weight, birch salt 87% by weight, garlic extract 4 A composition for health functional foods of Example 1 was prepared by mixing wt%, 0.8 wt% malt extract, 0.8 wt% gold silver hwa extract, 0.8 wt% pogongyeong extract, 0.8 wt% Yugeunpi extract, and 0.8 wt% Baekgangjam extract.

Comparative Example 1

The Nandamban prepared in Preparation Example 1 was used alone to prepare a health functional food composition of Comparative Example 1.

Comparative Examples 2 to 8

Instead of the Nandamban prepared in Preparation Example 1, the same amount of birch salt prepared in Preparation Examples 2 to 8 (Comparative Example 2), garlic extract (Comparative Example 3), malt extract (Comparative Example 4), gold and silver extract (Comparative Example 5), Pogongyeong extract (Comparative Example 6), Yugeunpi extract (Comparative Example 7), Baekgangjam extract (Comparative Example 8) was used alone, respectively, to prepare a composition for health functional food of Comparative Examples 2 to 8.

<Experimental Example>

Efficacy was evaluated through the experiments of Experimental Examples 1 to 5 as follows with respect to the sample of the composition for health functional food prepared in Example 1 and Comparative Examples 1 to 8.

Experimental Example 1. Antioxidant ability evaluation experiment

DPPH (2,2-diphenyl-1-picrylhydrazyl), a very stable free radical, was added to a hydrogen proton-radical scavenger to analyze antioxidant activity that affects the treatment of atopic dermatitis. The electron donating ability of each sample was measured using the phenomenon of discoloration from purple to yellow 1,1-diphenyl-2-picrylhydrazine.

Experimental method: After dissolving the samples of Example 1 and Comparative Examples 2 to 8 in purified water at concentrations of 0.1, 0.05, 0.025, 0.0125, 0.00625%), 75 μl each was added to 750 μl of DPPH (0.2 mM in ethanol) solution and 675 μl of tertiary purified water. Mixed with. After reacting at room temperature for 30 minutes, 200 μl of the reaction solution was transferred to a 96 well plate, and absorbance was measured at a wavelength of 520 nm with an ELISA reader device (Molecular Devices, CA, USA).

The DPPH radical scavenging activity (%) of each sample was calculated by the following formula (1).

[Equation 1]

DPPH radical scavenging activity (%) = 1-(absorbance of the sample added group/absorbance of the no sample added group X 100

division Raw material SC ₅₀ value (mg/ml) Example 1 Mixed composition 0.11 ± 0.02 Comparative Example 1 Retreat 0.94 ± 0.07 Comparative Example 2 Bamboo salt 0.80 ± 0.01 Comparative Example 3 Garlic extract 0.24 ± 0.06 Comparative Example 4 Malt extract 0.48 ± 0.02 Comparative Example 5 Gold and silver extract 0.17 ± 0.01 Comparative Example 6 Pogongyoung extract 0.15 ± 0.02 Comparative Example 7 Yugeun blood extract 0.78 ± 0.03 Comparative Example 8 White River Extract 1.91 ± 0.21

As can be seen from Figure 1 and Table 1, the sample of Example 1 is the minimum value for scavenging DPPH radicals 50% compared to the samples of Comparative Examples 1 to 8, the SC ₅₀ value is 0.11 ± 0.02 mg / ml, the antioxidant activity is the most It was excellent.

In addition, from the above results, Example 1 of the present invention implements remarkably improved antioxidant activity compared to the degree achievable through mixing of the samples of Comparative Examples 1 to 8, and a complex synergistic antioxidant activity by the total composition It can be seen that there is an improvement effect.

Experimental Example 2. Measurement of inhibition of enzyme activity of COX-2 (cyclooxygenase-2)

To investigate the effect of inhibiting the activity of COX-2 (cyclooxygenase-2), a major enzyme that causes inflammation in atopic dermatitis, arachidonic acid, a substrate of COX-2 enzyme, was used to determine the effect of hematin. It was measured using the phenomenon of discoloration by inducing oxidation.

Experimental method: COX-2 enzyme 10μl, heme 10μl, reaction buffer 950μl were added to a 1.5ml tube, 20μl of sample was added, stabilized at 37℃ for 5 minutes, arachidonic acid 10μl was added and reacted at 37℃ for 2 minutes, and then 1M HCl 50 μl was added and the reaction was stopped. Add 100 μl of SnCl _2, mix well, stabilize at room temperature for 5 minutes, dilute the reaction solution 2000 times and 4000 times with EIA buffer, and dispense 50 μl into a 96 well plate coated with prostaglandin antibody, and 50 μl of PGs screening AChE tracer and 50 μl of Antiserum After addition, it was reacted at room temperature for 18 hours. After washing the 96 wells 5 times with a buffer solution, 200 μl of Ellman's reagent was added and color was developed for 60 minutes, and the absorbance was measured at a wavelength of 410 nm. The degree of inhibition of COX activity was expressed as an IC ₅₀ value, which is a concentration that inhibits 50% according to the manufacturer's method.

division Raw material IC ₅₀ value (μg/ml) Example 1 Mixed composition 87.29 ± 5.83 Comparative Example 1 Retreat 94.61 ± 18.45 Comparative Example 2 Bamboo salt 292.6 ± 15.62 Comparative Example 3 Garlic extract 145.2 ± 28.10 Comparative Example 4 Malt extract 185.6 ± 21.05 Comparative Example 5 Gold and silver extract 135.5 ± 11.91 Comparative Example 6 Pogongyoung extract 117.9 ± 8.84 Comparative Example 7 Yugeun blood extract 402.4 ± 52.18 Comparative Example 8 White River Extract 158.1 ± 44.40

As can be seen from Fig. 2 and Table 2, the sample of Example 1 has an IC ₅₀ value of 87.29 ± 5.83 μg/ml, which is the minimum value that inhibits COX-2 by 50% compared to the samples of Comparative Examples 1 to 8. Inducing inhibitory effect was the best.

In addition, from the above results, Example 1 of the present invention implements a remarkably improved COX-2 inhibitory effect compared to the degree achievable through mixing of the samples of Comparative Examples 1 to 8, and the composite increase by the total composition It can be seen that it has an anti-inflammatory effect.

Experimental Example 3. Intracellular expression inhibition experiment of COX-2 (cyclooxygenase-2)

COX is divided into COX-1, which is expressed in various types of cells to regulate normal physiological functions, and COX-2, which is expressed in inflammation-related cells such as macrophages and mast cells by inflammation. In atopic dermatitis, COX is mainly -2 is expressed in large quantities. COX-2 uses arachidonic acid as a substrate to produce prostaglandin or leukotriene, a mediator of lipid inflammation and pain.

Therefore, in the present invention, the effect of inhibiting the expression of the COX-2 protein according to the concentration of Example 1 in Raw 264.7 cells stimulated with LPS was confirmed using immunocytochemistry (Immunocytochemistry).

Experimental method: Put a 12mm coverslip in a 24well plate and seed RAW 264.7 cells at a concentration of 1×10 ⁴ cells/ml at 1 ml/well and incubate (at 37°C, for 24 hours), treat samples at each concentration, and after 1 hour LPS (1μg/ml) and incubated again (at 37℃, for 24 hours), the medium was washed with PBS, fixed with 4% formalin, and secondary antibody with COX-2 (Cell signaling Technology, MA, USA) The antigen was labeled using an antibody (Santa Cruz Biotechnology, CA, USA), developed with DAB, and background stained with Mayer's hematoxylin.

The Raw 264.7 cells were treated with only phosphate buffered saline (PBS) as a normal cell group, and LPS (1 μg/ml) alone was treated to prepare a control group. In addition, the positive control treated with LPS (1 μg/ml) and Ibuprofen (50 μg/ml), and LPS (1 μg/ml) and Example 1 at concentrations of 10 mg/mL, 20 mg/mL, and 40 mg/mL, respectively An experimental group treated with was prepared.

The normal cell group (PBS), control (LPS), positive control (IBU) and experimental group 1-1 (10 mg/mL), 1-2 (20 mg/mL), 1-3 (40 mg/mL) treated with Example 1 ) The results of immunocytochemical staining of COX-2 are shown in FIG. 3.

As can be seen from FIG. 3, it was confirmed that the expression of COX-2 was suppressed in a concentration-dependent manner in the experimental group treated with Example 1 of the present invention.

Experimental Example 4. Intracellular expression inhibition experiment of iNOS (inducible nitric oxide synthase)

In inflammatory reactions such as atopic dermatitis, macrophages and mast cells overexpress iNOS (inducible nitric oxide synthase) and secrete excess NO (nitric oxide) out of the cells, thereby amplifying the inflammatory reaction.

Therefore, in the present invention, the effect of inhibiting the expression of the iNOS protein according to the concentration of Example 1 in Raw 264.7 cells stimulated with LPS was confirmed by using immunocytochemistry.

Experimental method: Put a 12mm coverslip in a 24 well plate and seed RAW 264.7 cells at a concentration of 1×10 ⁴ cells/ml at 1 ml/well, then incubate (at 37℃, for 24 hours), and treat the samples at each concentration. After an hour, LPS (1 μg/ml) treatment, culture (at 37°C, for 24 hours), the medium was washed with PBS, fixed with 4% formalin, and the primary antibody, iNOS (Santa Cruz Biotechnology, CA, USA) And a secondary antibody (Santa Cruz Biotechnology, CA, USA) to label the antigen and develop color with DAB. Background staining was performed with Mayer's hematoxylin.

The Raw 264.7 cells were treated with only phosphate buffered saline (PBS) as a normal cell group, and LPS (1 μg/ml) alone was treated to prepare a control group. In addition, the positive control treated with LPS (1 μg/ml) and Ibuprofen (50 μg/ml), and LPS (1 μg/ml) and Example 1 at concentrations of 10 mg/mL, 20 mg/mL, and 40 mg/mL, respectively An experimental group treated with was prepared.

The normal cell group (PBS), control (LPS), positive control (IBU) and experimental group 2-1 (10 mg/mL), 2-2 (20 mg/mL), 2-3 (40 mg/mL) treated with Example 1 ) Is shown in Fig. 4 shows the results of immunocytochemical staining of iNOS.

As can be seen from Figure 4, it was confirmed that the expression of iNOS was suppressed in a concentration-dependent manner in the experimental group treated with Example 1 of the present invention.

Experimental Example 5. Atopic skin disease improvement efficacy test using NC/Nga mice

Atopic animal model was induced by applying DNCB (2.4-Dinitrochlorbenzene), which causes atopic dermatitis, to NC/Nga mice for 6 weeks, and histopathological examination (skin tissue lesion observation, mast cells observation, Eosinophils observation) was performed. .

Experimental method: To induce atopic dermatitis, first hair removal was performed on mice, etc. using an electric razor, and second hair removal was performed using a hair removal cream to completely remove hair. On the 6th day after acquisition in the first induction stage, 200 μl 1% DNCB was applied to the depilated skin. In the second induction stage, 200 µl 1% DNCB was applied to the depilated skin on the second day after the first sensitization. In the challenge stage, from the 4th day after the second sensitization, 150µl 0.4% DNCB was applied to the depilated skin and repeatedly applied 3 times a week (Mon/Wed/Fri) for 6 weeks to induce dermatitis.

For the administration of the test substance, the test substance and the positive control substance were forcibly administered orally using the sonde for oral administration. The number and time of administration were administered once/day, 7 days/week, 4 weeks, and before 16:00 on the day of administration. The dose amount was calculated as a dose amount of 10 ml/kg based on the most recently measured body weight. The composition of the test group is shown in Table 3 below.

group gender Number of animals
(Marie) Dosage
(Ml/kg) Example 1 dosage
(Mg/kg/day) G1 Normal control Male 10 10 0 G2 AD control Male 10 10 0 G3 AD Example 1 (low concentration) Male 10 10 200 G4 AD Example 1 (medium concentration) Male 10 10 400 G5 AD Example 1 (high concentration) Male 10 10 800 G6 AD positive control Male 10 10 0 * G1: normal control, G2: induction control, G3～G5: induction test group, G6: induction positive control
* Atopic dermatitis (AD)

After autopsy, the skin tissue is cut and histopathological examination is performed. To check the lesions of the skin tissue, hematoxylin & eosin stains are performed, and the mast cells and congo red stains are performed with toluidine-blue staining to check the degree of eosinophil infiltration. On H&E staining, it is classified into hyperplasia, dermal, inflammtion, dermal, erosion/ulcer, epidermal, hyperkeratosis, and fibrosis, dermal, respectively. Scores 0-4 (0: normal, 1: minimal, 2: mild, 3: moderate, 4: severe) was evaluated, and the results are shown in Table 4 below.

In addition, the results of the atopic dermatitis animal model of Experimental Example 5 in FIG. 5 and the histopathological test results in the atopic dermatitis animal model of Experimental Example 5 are shown in FIG.

GROUP(mg/kg) Histopathological criteria G1(0) G2(0) G3(200) G4(400) G5(800) G6(1) Hyperplasia, epidermal 0.00 2.70 2.30 2.22 1.60 1.80 Inflammation, dermal 0.00 1.70 1.40 1.11 1.00 0.60 Erosion/Ulcer, epidermal 0.00 0.30 0.10 0.33 0.20 0.20 Hyperkeratosis 0.00 1.00 1.00 1.00 0.70 1.00 Fibrosis, dermal 0.00 1.10 1.00 1.11 0.60 1.00 Total score 0.00 6.80 5.80 5.78 4.10 4.60

* G1: normal control, G2: induction control, G3～G5: induction test group, G6: induction positive control

As can be seen from Table 4 and FIGS. 5 to 6, as a result of observing the lesion of the skin tissue, the normal control group did not have any abnormal findings, so all the item scores were calculated as 0.00 points, and the triggering control group that caused only atopic dermatitis was In terms of epidermal hyperplasia and dermal inflammation, it showed the highest score compared to other test groups. In the induction test group, scores of epidermal hyperplasia and dermal inflammation tended to decrease from low concentration to high concentration. In particular, hyperkeratosis and dermal fibrosis scores tended to decrease in the 800 mg/kg/day group. In addition, the triggered control group showed the highest score in the total sum score, and the calculated score tended to decrease as the concentration increased.

Experimental Example 6. in vitro Genotoxicity test (reversion mutation test)

In order to confirm the mutagenicity of the microorganism of Example 1, the histidine-requiring strains Salmonella typhimurium TA98, TA100, TA1535, and TA1537 and the tryptophan-requiring strain Escherichia coli WP2uvrA were used to test for return mutations by direct method and metabolic activity method. Was carried out.

Ministry of Food and Drug Safety Notification No. 2015-82 (November 11, 2015)'Standards for Toxicity Tests for Pharmaceuticals', OECD Guideline for Testing of Chemicals No. The test was conducted according to 471'Bacterial Reverse Mutation Test' (21st July 1997). Preincubation method was used, and the method of applying and not applying the metabolic activity system was carried out in parallel.

Observation, measurement, inspection and analysis methods are as follows.

(1) Measurement of the number of colonies

Colonies generated on a 90 mm diameter plate (86 mm inner diameter) were counted using a colony counter (Model 570, SUNTEX, Taiwan).

(2) How to check whether growth is inhibited

All plates were observed and confirmed with the naked eye.

(3) Method of measuring bacterial concentration

The bacterial concentration was measured according to the method of measuring the number of viable cells by the step dilution method in the standard work manual of this laboratory.

(4) Sterility test

0.1 ml of S9 mix and test substance solvent were layered on the minimum glucose agar medium with top agar, and incubated for 48 hours at 37°C. As a result, it was confirmed that the bacteria did not grow. Through this, it could be seen that the test was performed aseptically.

Judgment of test result: The result of this test is the actual measured value, average value and standard deviation of the number of colonies of the return mutant of each plate. The determination of the results was determined as positive when the number of colonies returned per plate in at least one strain, regardless of the presence or absence of the metabolic activity system, was concentration dependent or showed a reproducible increase in one or more concentrations. However, it should show a clear increase of more than two times compared to the negative control group.

In this test, as a result of treating each concentration of the test substance, there was no increase in the number of colonies that could be judged as positive in the case of the direct method compared to the negative control group. In the case of the metabolic activity method, cytotoxicity was observed at the concentration of 5000 μg/plate of the TA1537 strain, and no increase in the number of return colonies that could be judged as positive was observed in all strains compared to the negative control group. Through the above results, it was determined that Example 1 did not induce a return mutation under this test condition.

Experimental Example 7. in vitro Genotoxicity test (chromosome abnormality test)

In order to evaluate the genotoxicity of chromosomal abnormalities of Example 1, metabolic activation method (+S9 mix) and direct method without applying metabolic activating enzyme system (S9) using Chinese hamster-derived ovarian infant cells (CHO-k1 cells) ( -S9 mix) chromosomal abnormality test was performed. The test substance was prepared by dispersing in sterile distilled water and then diluting.

In order to determine the treatment concentration of the test substance in the main test, a cell proliferation inhibition test was performed, and the maximum treatment concentration of the test substance for the main test was determined, and the concentration group of the three levels of azeotrope 3 was determined as follows.

Direct method (-S9 mix, 24-hour continuous treatment group): 61.73, 185.19, 555.56 ㎍/㎖

Direct method (-S9 mix, 6 hours treatment 18 hours recovery group): 61.73, 185.19, 555.56 ㎍/㎖

Metabolic activity method (+S9 mix, 6 hours treatment 18 hours recovery group): 6.86, 20.58, 61.73 ㎍/㎖

As a result of this test, in the 24-hour continuous treatment group and the 6-hour treatment 18-hour recovery group of the direct method without metabolic activation, no statistically significant increase was observed at all treatment concentrations compared to the negative control group. .

In the case of the metabolic activity method (6 hours treatment and 18 hours recovery group), no statistically significant increase was observed in the frequency of abnormal intermediate phases in all treatment groups compared to the negative control group.

In the direct method and metabolic activity method, the frequency of ploidy (PP) and endonuclearization (ER) did not show statistically significant increase compared to the negative control group (Tables 1 & 2). However, in the positive control group, the frequency of abnormal intermediate phases showed a statistically significant increase compared to the solvent control group (p<0.05).

Taking the above results together, it is judged that Example 1 of the present invention does not induce chromosomal abnormalities in CHO-k1 cells under this test condition.

Experimental Example 8. in vitro Genotoxicity test (micronucleus test)

In order to obtain the basic data for determining the presence or absence of micronucleus induction for Example 1, a micronucleus test was performed using ICR mouse bone marrow cells during the genotoxicity test.

Male mice of about 8 weeks of age were administered orally twice for 2 days with doses of 2000 mg/kg bw/day, 1000 mg/kg bw/day, and 500 mg/kg bw/day, Bone marrow cells were collected 18 to 24 hours after administration to evaluate micronucleus induction frequency and cytotoxicity.

As a result of counting the number of MNPCEs targeting about 4000 PCEs per individual within the dose range applied in this study, all test substance administration groups (2000 mg/kg bw/day, 1000 mg/kg bw/day, 500 mg/kg bw /day) showed no statistically significant difference compared to the solvent control group. On the other hand, the micronucleus incidence frequency of the positive control group was significantly increased compared to that of the solvent control group (p<0.01).

The ratio of PCE/(PCE+NCE) among 500 red blood cells, which is an index of cytotoxicity, did not show any significant inhibition of bone marrow cell proliferation compared to the solvent control group in all test substance administration groups.

As a result of the above results, it is considered that Example 1 of the present invention does not induce micronuclei in mouse bone marrow cells under this test condition.

Experimental Example 9. in vivo Single oral administration toxicity test

This test was conducted to investigate the toxicity symptoms and the approximate lethal dose when oral administration of the test substance antifoam kneading salt to Sprague-Dawley (SD) male and female rats. The test substance was set to a dose of 320 mg/kg (low dose group), 800 mg/kg (medium dose group) and 2,000 mg/kg (high dose group) and compared with the excipient control group. General symptoms, weight change and autopsy findings of surviving animals were observed at the end of the experiment.

1) No dead animals were observed during the experiment, and salivation occurred immediately after administration of the test substance in the test substance administration groups of 800 mg/kg and 2,000 mg/kg of male and female.

2) As a result of weight measurement, normal weight gain was observed in all animals, and no statistically significant change was observed between the test groups.

3) At the end of the experiment, no peculiar macroscopic findings were observed as a result of autopsy of all surviving animals.

As a result of the above results, a single oral administration of the test substance using rodent Sprague-Dawley rats, no dead animals were observed, and no specific changes related to the administration of the test substance were observed.

= National project information =

Assignment identification number (national management number): S2424212

Ministry name (contributing institution): Ministry of Small and Medium Venture Business

Research Management Professional Institution: Small and Medium Business Technology Information Promotion Agency

Research Project Name (National Research Project Name): Technology Innovation Development Project

Research Project Name (National Research Project Title): Development and commercialization of new functional materials for improving atopic immune hypersensitivity dermatitis using medicinal compositions derived from natural products

Contribution Rate: 1/1

Organizer: Insan Bamboo Salt Co., Ltd.

Research period (project start date ~ project end date): September 1, 2016-August 31, 2018

Claims (5)

Nandamban 0.1% to 50% by weight, birch salt 1% to 90% by weight, garlic extract 1% to 20% by weight, malt extract 0.5% to 40% by weight, gold and silver extract 0.5% to 5% by weight, A complex mixture containing 0.5% to 5% by weight of Pogongyeong extract, 0.5% to 5% by weight of Yugeunpi extract, and 0.5% to 5% by weight of Baekanthus extract as active ingredients
For the Nandamban, Jajuk salt is added in a midrange ratio of 1: 17.4,
The complex mixture is a health functional food composition for improving atopic dermatitis, characterized in that it inhibits the activity of the enzyme COX-2 (cyclooxygenase-2).
The method of claim 1,
The Nandamban is a health functional food composition for improving atopic dermatitis by mixing the heated and dehydrated gallbladder powder and egg white of eggs in a weight ratio of 1:0.2 to 1:1.
delete Preparing each of Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract; And
Preparing a complex mixture by mixing the respective Nandamban, Jajuk salt, garlic extract, malt extract, Geumeunhwa extract, Pogongyeong extract, Yugeunpi extract and Baekgangjam extract; Including,
In the preparing step, in the preparation of the Nandamban, the gallbladder is heated at 100°C to 150°C, dehydrated so that the moisture content is 0.001% to 5%, and then completely cooled at room temperature and then powdered to prepare the Gallban powder. The egg white of the egg is mixed with the gallbladder powder in a weight ratio of 1:0.2 to 1:1,
The complex mixture is Nandamban 0.1% to 50% by weight, Jajuk salt 1% to 90% by weight, 1% to 20% by weight of garlic extract, 0.5% to 40% by weight of malt extract, from 0.5% by weight of gold and silver extract It contains 5% by weight, 0.5% to 5% by weight of Pogongyeong extract, 0.5% to 5% by weight of Yugeunpi extract, and 0.5% to 5% by weight of Baekanthus extract,
Jajuk salt is added to the Nandamban at a midrange ratio of 1: 17.4,
The complex mixture is a method for producing a composition for a health functional food for improving atopic dermatitis, characterized in that it inhibits the activity of the enzyme COX-2 (cyclooxygenase-2).


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