KR20220049671A - Composition for enhancing skin cell regeneration or wound healing containing absolute of Miscanthus sinensis var. purpurascens flower - Google Patents

Abstract

The present invention relates to a composition for enhancing skin regeneration or wound healing containing Miscanthus sinensis flower essential oil, and more specifically, to a cosmetic composition for skin regeneration or wound healing of essential oil extracted from flowers of Miscanthus sinensis by solvent extraction, and a composition for external application to the skin. The present invention identifies components of essential oil extracted from flowers of Miscanthus sinensis, induces proliferation and migration of keratinocytes, and induces type I and type IV collagen synthesis and secretion. Therefore, the present invention has an excellent effect on wound healing or skin regeneration.

Description

Composition for enhancing skin cell regeneration or wound healing containing absolute of Miscanthus sinensis var. purpurascens flower}

The present invention relates to a composition for skin regeneration or wound healing containing essential oil of pampas grass flower, and more particularly, to skin regeneration or wound healing of essential oil extracted from Miscanthus sinensis var. purpurascens flowers by solvent extraction. It relates to a cosmetic composition for use or a composition for external application to the skin.

The skin is the largest organ in the human body and forms a physical, chemical, and immunological barrier to protect the human body from infections and foreign substances and is composed of the epidermis, dermis, and subcutaneous fat layer. In addition, the skin is essential for maintaining homeostasis and performs various functions such as temperature regulation, suppression of water loss, containing sensory receptors, synthesizing various biochemicals, and excreting small amounts of waste products. The epidermis, which exists at the outermost part of the structure of the skin, is composed of the basal layer, the stratum corneum, the granular layer, and the stratum corneum, and about 80% is composed of keratinocytes. Merkel cells are responsible for The proliferation of keratinocytes plays an important role in enhancing skin elasticity and forming the basement membrane by inducing wound healing, skin regeneration, and collagen (collagen type I, collagen type IV) synthesis. The dermis layer exists between the epidermis layer and the fat layer and occupies more than 90% of the skin, and is an elastic tissue with viscoelasticity. The dermal layer is composed of fibrous proteins such as collagen fiber, elastin fiber, and ground substance, and plays a role in protecting other tissues and epidermal layers by supplying nutrients. In human skin, collagen I, III, IV, V, VI, and VII 6 types are distributed, of which type I accounts for about 85%. It is known that the synthesis of collagen is involved in wound healing, various fibrosis and inflammatory processes, gives elasticity and elasticity to the skin, and improves the formation of the basement membrane, thereby regulating the adhesion and proliferation of skin cells. For this reason, the migration and proliferation of epidermal keratinocytes and the induction of collagen synthesis help to promote skin elasticity and maintain healthy skin as well as skin regeneration and wound healing.

Silver grass ( Miscanthus sinensis var. purpurascens ) is a perennial plant of the Grapeaceae that grows in East Asia such as Korea (all regions), China, and Japan. The height is 1-2 m, and the rhizomes are gathered, thick, and cylindrical. It has a leaf shape with a width of 1~2cm and a length of 40~70cm, and it is sharp at the tip and has several veins, but the veins in the middle are white and thick. The base is made of a leaf sheath and has long hairs, and the flowers form a fan-shaped or corymbal inflorescence toward the tip of the stem in autumn, with ears running. Silver grass has been used to improve blood circulation through detoxification, vasodilation, diuresis, and antipyretic functions since ancient times, and contains physiologically active compounds such as miscanthosides and 4-hydroxybenzaldehyde, which are a kind of polyphenol.

Essential oils derived from natural products are secondary metabolites stored in the space of plants, such as flowers, leaves, fruits, skins and roots. It is composed of organic compounds. The essential oil has a unique fragrance depending on the plant species or part, and the therapeutic ingredients of the oil show effects such as tension relief, calming, stress relief, fatigue recovery, and muscle relaxation through the skin, circulatory system and olfactory system, as well as antibacterial and immune enhancement. Since it has anti-allergic action, it has been used for the treatment and prevention of diseases since a long time ago, and it is also effective in beauty such as preventing aging. Therefore, as problems such as high toxicity to the human body and hormonal disturbance of various chemicals used in cosmetics and quasi-drugs have recently emerged, the market for natural fragrances is expanding and the market for natural fragrances is expanding in various industries. being applied in the field. As such, natural materials are essential, and research related to the development of functional materials derived from natural products is required.

Accordingly, the present inventors completed the present invention by confirming that the essential oil extracted from Pampas grass flowers by an organic solvent extraction method induces proliferation of keratinocytes and induces type I and type IV collagen synthesis and secretion.

Korean Patent Publication No. 10-2013-0067263

An object of the present invention is to provide a cosmetic composition for skin regeneration or wrinkle improvement.

Another object of the present invention is to provide a composition for external application for skin for promoting wound healing or skin regeneration.

Another object of the present invention is to provide a method for producing a pampas grass flower essential oil.

In order to achieve the above object,

The present invention provides a cosmetic composition for skin regeneration or wrinkle improvement comprising an essential oil extracted from Miscanthus sinensis var. purpurascens flowers as an active ingredient.

In addition, the present invention provides a composition for external application for skin for promoting wound healing or skin regeneration comprising an essential oil extracted from Miscanthus sinensis var. purpurascens flowers as an active ingredient.

Furthermore, the present invention includes the steps of obtaining solid concrete by concentrating the hexane (n-hexane) extract of Miscanthus sinensis var. purpurascens flowers (step 1); and

Dissolving the solid concrete of step 2 in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of pampas grass flower absolute (step 2); do.

The present invention identifies the components of essential oil extracted from Miscanthus sinensis var. purpurascens flowers, induces proliferation and migration of keratinocytes, induces type I and type IV collagen synthesis and secretion, and is useful for wound healing or skin regeneration. It has an excellent effect.

1 is a flowchart illustrating a process of extracting essential oil from Pampas grass flower according to an embodiment of the present invention.
Figure 2 is a graph showing the cell viability of the skin keratinocytes of essential oil of pampas grass flower according to an embodiment of the present invention.
Figure 3A is a graph showing the effect of essential oil of pampas grass flower on the proliferation of epidermal keratinocytes according to an embodiment of the present invention.
Figure 3B is a graph showing the effect of the essential oil of pampas grass flower on the migration of epidermal keratinocytes according to an embodiment of the present invention.
4A is a graph showing the effect of Pampas grass flower essential oil on collagen type I synthesis according to an embodiment of the present invention.
Figure 4B is a graph showing the effect of Pampas grass flower essential oil on collagen type IV synthesis according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

Cosmetic composition for skin regeneration or wrinkle improvement

The present invention relates to a cosmetic composition for skin regeneration or wrinkle improvement comprising an essential oil extracted from Miscanthus sinensis var. purpurascens flowers as an active ingredient.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower is

It can be prepared by extracting pampas grass flowers by solvent extraction to obtain concrete, dissolving it in an organic solvent and filtering it to remove insoluble wax to obtain absolute essential oil.

More specifically, the step of concentrating the hexane (n-hexane) extract of the pampas grass ( Miscanthus sinensis var. purpurascens ) to obtain a solid concrete; and

Dissolving the solid concrete in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of pampas grass flower absolute (absolute); can be prepared including.

The organic solvent extraction method and the method of separating the essential oil in the absolute form from the extract may include all methods that can be modified by a person of ordinary skill in the art.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower is 1-dotriacontanol, (1-Dotriacontanol), nonadecane (Nonadecane), tridecyl aldehyde (tridecanal; Tridecyl Aldehyde), octyldodecanol , (1-Dodecanol, 2-octyl-; Octyldodecanol), 1,2-epoxyoctadecane (Oxirane, hexadecyl-; 1,2-Epoxyoctadecane) or nonyl aldehyde (Nonanal; Nonyl Aldehyde) can

In one embodiment of the present invention, the pampas grass flower may be a flower that is not dried.

As used in the present invention, the term "essential oil" is a term that collectively refers to aromatic oils (volatile substances) extracted and separated from plants (leaf, stem, fruit, flower, root, etc. of plants), extract, extraction method, use and purpose are classified in various ways according to Essential oils derived from natural products are classified into essential oils, absolutes, concrete, and the like according to extraction methods.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower can be used in the range of 10 to 250 ㎍ / ㎖, preferably used in the range of 100 to 250 ㎍ / ㎖, more preferably 180 to 250 It can be used in the range of μg/ml.

The cosmetic composition of the present invention can be prepared by including a cosmetically effective amount of the essential oil extracted from the pampas grass flower of the present invention as described above and a cosmetically acceptable carrier.

The appearance of the cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. It is in any formulation suitable for topical application, for example solutions, gels, solids, kneaded dry products, emulsions obtained by dispersing the oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream , nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the essential oil extracted from the pampas grass flower. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extract.

In the cosmetic composition of the present invention, a component contained in a general cosmetic composition may be further blended with the functional additive as needed. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

Composition for external application for wound healing or promoting skin regeneration

The present invention relates to a composition for external application for skin for promoting wound healing or skin regeneration comprising, as an active ingredient, essential oil extracted from Miscanthus sinensis var. purpurascens flowers.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower is

It can be prepared by extracting pampas grass flowers by solvent extraction to obtain concrete, dissolving it in an organic solvent and filtering it to remove insoluble wax to obtain absolute essential oil.

More specifically, the step of concentrating the hexane (n-hexane) extract of the pampas grass ( Miscanthus sinensis var. purpurascens ) to obtain a solid concrete; and

Dissolving the solid concrete in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of pampas grass flower absolute (absolute); can be prepared including.

The organic solvent extraction method and the method of separating the essential oil in the absolute form from the extract may include all methods that can be modified by a person of ordinary skill in the art.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower is 1-dotriacontanol, (1-Dotriacontanol), nonadecane (Nonadecane), tridecyl aldehyde (tridecanal; Tridecyl Aldehyde), octyldodecanol , (1-Dodecanol, 2-octyl-; Octyldodecanol), 1,2-epoxyoctadecane (Oxirane, hexadecyl-; 1,2-Epoxyoctadecane) or nonyl aldehyde (Nonanal; Nonyl Aldehyde) can

In one embodiment of the present invention, the pampas grass flower may be a flower that is not dried.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower can be used in the range of 10 to 250 ㎍ / ㎖, preferably used in the range of 100 to 250 ㎍ / ㎖, more preferably 180 to 250 It can be used in the range of μg/ml.

In one embodiment of the present invention, the essential oil extracted from the pampas grass flower promotes the proliferation or migration of epidermal keratinocytes, and promotes the synthesis and secretion of type I and type IV collagen of epidermal keratinocytes, thereby promoting skin regeneration and wounding. It was confirmed that there is a healing effect (see Experimental Examples 2 to 4 and FIGS. 3 and 4).

In addition, the present invention includes the steps of obtaining solid concrete (concrete) by concentrating the hexane (n-hexane) extract of Miscanthus sinensis var. purpurascens flowers (step 1); and

Dissolving the solid concrete of step 2 in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of pampas grass flower absolute (step 2); do.

The present invention will be described in more detail through the following examples. However, the following examples are only intended to embody the contents of the present invention, and the present invention is not limited thereto.

<Example 1> Preparation of essential oil of pampas grass flower

According to the process shown in FIG. 1, essential oil was prepared from pampas grass flowers. In this example, pampas grass flowers were collected and used in Asan, Chungcheongnam-do.

That is, silver grass flowers were precipitated in hexane (n-hexane), and solid concrete was obtained by concentrating the extract of the precipitate thus obtained. Then, after dissolving the concrete by adding ethyl alcohol, the insoluble wax was removed through filtration, and again separated/refined to prepare a pampas grass flower essential oil. And the prepared pampas grass flower essential oil was stored at a low temperature of -80 ℃.

<Example 2> Analysis of components of pampas grass flower essential oil

Analysis of the components of Pampas grass flower essential oil was commissioned by KBSI (Korea Basic Science Institute, Seoul Center). A gas chromatography/mass spectrometer (7890BGC/ 7010QQQ, Agilent, USA) was used, and a DB5-MS (30 m×0.25 μm, 0.25 μm) was used for the GC/MS column, and a carrier gas was used. ) was analyzed by using helium to flow at 1 mL/min. The temperature of the oven was maintained at 40°C-2°C/min-230°C-5°C/min-300°C (15 minutes), and the injection temperature was analyzed at 300°C. In addition, the temperature of the interface was analyzed at 300℃, the temperature of the ion source at 300℃, and the temperature of the analyzer at 230℃, and the mass range was analyzed at 50~800 m/z.

To confirm the components of pampas grass essential oil, the retention indices (RI) of the volatile component and the retention time of the standard material were matched, or the material was confirmed by comparing the mass spectrum of the volatile material and the mass spectrum of the Wiley 7Nist 05 library.

RI was determined under the same conditions as the sample using C ₆ ~ C ₂₄ (n-alkane). As a result, it was confirmed that it was composed of a total of six components, and 1-Dotriacontanol showed a high content in the order of 48.72% and Nonadecane 21.84% (Table 1). reference).

No component name retention time Area (%) Cas No. One nonyl aldehyde
(Nonal; Nonyl Aldehyde) 23.298 3.22 124-19-6 2 Tridecyl Aldehyde (tridecanal; Tridecyl Aldehyde) 94.133 10.69 10486-19-8 3 Nonadecane 97.092 21.84 629-92-5 4 1,2-Epoxyoctadecane (Oxirane, hexadecyl-; 1,2-Epoxyoctadecane) 101.435 5.12 7390-81-0 5 Octyldodecanol (1-Dodecanol, 2-octyl-; Octyldodecanol) 102.548 10.40 2000526-48-2 6 1-Dotriacontanol
(1-Dotriacontanol) 109.85 48.72 6624-79-9

<Experimental Example 1> Investigation of cell viability effect of pampas grass flower essential oil on keratinocytes

The cell viability of the pampas grass flower essential oil obtained in Example 1 on the epidermal keratinocytes was investigated.

Keratinocytes (HaCat) were cultured using DMEM medium containing 10% fetal bovine serum and used in the experimental example of the present invention. Keratinocytes (HaCaT) were placed in a 96 well-plate so as to be 5 X 10 ³ cells/100 μL/well, and then cultured at 37° C., 5% CO ₂ in an incubator for 12 hours. After 12 hours, 100 μL in each well was treated at a concentration of 1 ~ 300 μg/mL of pampas grass flower essential oil, and cultured for 24 hours. ) was used to measure the absorbance at 450 nm, and the results are shown in FIG. 2 .

As a result, as shown in FIG. 2, cytotoxicity was not observed at a concentration of 1-300 μg/mL of pampas grass essential oil, and cell viability was increased at a concentration of 100-300 μg/mL. Therefore, the concentration of pampas grass essential oil according to the present invention is 300 μg/ml The following concentrations are preferred.

<Experimental Example 2> Effect of pampas grass flower essential oil on the proliferation of keratinocytes

In order to confirm the effect of the pampas grass flower essential oil obtained in Example 1 on the proliferation of epidermal keratinocytes, it was investigated as follows.

Human skin-derived keratinocytes (HaCat) were cultured using DMEM medium containing 10% fetal bovine serum and used in the experimental examples of the present invention. Keratinocytes (HaCaT) were placed in a 96 well-plate so as to become 3 X 10 ³ cells/100 μL/well, and then cultured at 37° C., 5% CO ₂ in an incubator for 12 hours. After 12 hours, 100 μL of each well was treated at a concentration of 10 ~ 250 μg/mL of pampas grass flower essential oil, and cultured for 48 hours. USA) was used to measure the absorbance at 450 nm.

Here, the negative control group did not use pampas grass flower essential oil, and the positive control group used epidermal growth factor (EGF 50 ng/mL) conventionally used for wound healing and/or skin regeneration.

As a result, as shown in FIG. 3(A), it was found that the Pampas grass flower essential oil according to the present invention induces the proliferation of epidermal keratinocytes in a concentration-dependent manner. That is, the proliferation of epidermal keratinocytes by epidermal growth factor (50 ng/mL) was induced by 381.68±6.62%, Compared with the positive control group, the growth induction rate of 190.73±2.76% at the concentration of 150 μg/mL Pampasea flower essential oil was shown, and the maximum proliferation induction rate of 327.15±12.67% at the concentration of 250 μg/mL, resulting in proliferation of keratinocytes. has been shown to effectively induce

<Experimental Example 3> Effect of pampas grass flower essential oil on keratinocyte migration

In order to confirm the effect of the pampas grass flower essential oil obtained in Example 1 on the migration of epidermal keratinocytes, an experiment was conducted as follows.

Keratinocytes (HaCat) were cultured using DMEM medium containing 10% fetal bovine serum and used in the experimental example of the present invention. The migration rate of epidermal keratinocytes was investigated using a 48-well Boyden microchemotaxis chamber. First, the lower chamber was treated with 10 ~ 250 μg/mL of pampas grass flower essential oil, respectively, and 8 μm pore polycarbonate coated with type I collagen. membrane was raised. Thereafter, the upper chamber was treated with 1×10 ⁶ cells/50 μL of keratinocytes per well. After incubating the chamber in an incubator at 37° C. for 3 hours and 30 minutes, the membrane was stained with Diff-Quick solution and fixed.

The number of cells that migrated through the membrane was counted and converted into a percentage to obtain the results for the migration of keratinocytes in the skin of pampas grass flower essential oil. The negative control group did not use pampas grass flower essential oil, and the positive control group used epidermal growth factor (EGF 1 ng/mL) which is conventionally used for wound healing and/or skin regeneration.

As a result, as shown in FIG. 3(B) , the Pampas grass flower essential oil according to Example 1 of the present invention induced the migration of keratinocytes in a concentration-dependent manner, and in particular, at a concentration of 150 μg/mL At a concentration of 220.63±17.79% and 200 μg/mL, it showed a migration rate of 290.48±25.22%, indicating that pampas grass flower essential oil effectively induces the migration of keratinocytes.

<Experimental Example 4> Effect of pampas grass flower essential oil on the synthesis and secretion of type I or IV collagen in keratinocytes

In order to measure the effect of the pampas grass flower essential oil obtained in Example 1 on the collagen synthesis and secretion of keratinocytes, the concentration of the pampas grass flower essential oil presented in Experimental Example 1 was investigated as follows.

A 96-well microtiter plate was coated with monoclonal anti-collagen I or IV Ab (2 μg/well/PBS) at room temperature for 12 hours after injection. After blocking with blocking buffer for 1 hour, each sample was treated with 100 μL per well and reacted for 90 minutes. The sample was treated with a culture medium (CM) of epidermal keratinocytes that were not treated with Pampasea flower essential oil (CM) and a culture medium (CM) of epidermal keratinocytes treated with 100 μg/mL and 200 μg/mL of Pampasa flower essential oil. After that, 100 μL of polyclonal anti-collagen I or IV Ab was treated in each well and reacted for 90 minutes, and then avidin-HRP was treated in each well and reacted for 60 minutes at room temperature. After injecting 100 μL of ELISA pico chemiluminescent substrate into each well and reacting for 10 minutes, the degree of luminescence was measured using an ELISA plate reader. The results were obtained.

As a result, as shown in FIG. 4(A), Pampas grass essential oil significantly promoted the synthesis and secretion of type I collagen in epidermal keratinocytes. In particular, it was shown that the synthesis and secretion of collagen type I were induced by 183.25±7.93% at a concentration of 200 μg/mL in the culture medium (CM) of epidermal keratinocytes.

In addition, as shown in FIG. 4(B), Pampasea essential oil significantly promoted the synthesis and secretion of type IV collagen in epidermal keratinocytes. In particular, it was shown that the synthesis and secretion of collagen type IV was induced by 149.63±4.56% at a concentration of 200 μg/mL in the epidermal keratinocyte culture medium (CM).

So far, the present invention has been looked at with respect to preferred embodiments thereof.

Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (12)

Miscanthus sinensis var. purpurascens A cosmetic composition for skin regeneration or wrinkle improvement comprising essential oil extracted from flowers as an active ingredient. According to claim 1,
The essential oil extracted from the pampas grass flower is
Pampas grass ( Miscanthus sinensis var. purpurascens ) Concentrating the hexane (n-hexane) extract of the flower to obtain a solid concrete; and
Dissolving the solid concrete in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of pampas grass flower absolute (absolute). According to claim 1,
The essential oil extracted from the pampas grass flower is 1-dotriacontanol, (1-Dotriacontanol), nonadecane (Nonadecane), tridecyl aldehyde (tridecanal; Tridecyl Aldehyde), octyldodecanol, (1-Dodecanol, 2-octyl -; Octyldodecanol), 1,2-epoxyoctadecane (Oxirane, hexadecyl-; 1,2-Epoxyoctadecane) and nonyl aldehyde (Nonanal; Nonyl Aldehyde) skin regeneration characterized in that it contains one or more components selected from the group consisting of Or a cosmetic composition for improving wrinkles. According to claim 1,
The essential oil extracted from the pampas grass flower is a cosmetic composition for skin regeneration or wrinkle improvement, characterized in that it is contained in the range of 10 to 250 ㎍ / ㎖. 5. The method of claim 4,
The cosmetic composition for skin regeneration or wrinkle improvement, characterized in that the essential oil extracted from the pampas grass flower is included in the range of 100 to 250 ㎍ / ㎖. A composition for external application for skin for promoting wound healing or skin regeneration, comprising, as an active ingredient, essential oil extracted from Miscanthus sinensis var. purpurascens flowers. 7. The method of claim 6,
The essential oil extracted from the pampas grass flower is
Pampas grass ( Miscanthus sinensis var. purpurascens ) Concentrating the hexane (n-hexane) extract of the flower to obtain a solid concrete; and
Dissolving the solid concrete in ethyl alcohol to remove the insoluble wax and concentrating to obtain an essential oil in the form of pampas grass flower absolute (absolute) composition. 7. The method of claim 6,
The composition for external application for skin application for promoting wound healing or skin regeneration, characterized in that the essential oil extracted from the pampas grass flower is included in the range of 10 to 250 μg/ml. 9. The method of claim 8,
The composition for external application for skin for promoting wound healing or skin regeneration, characterized in that the essential oil extracted from the pampas grass flower is included in the range of 100 to 250 μg/ml. 7. The method of claim 6,
The composition for external application for skin for promoting wound healing or skin regeneration, characterized in that the essential oil extracted from the pampas grass flower promotes the proliferation or migration of epidermal keratinocytes. 7. The method of claim 6,
The composition for external application for skin application for promoting wound healing or skin regeneration, characterized in that the essential oil extracted from the pampas grass flower promotes the synthesis and secretion of type I and type IV collagen in epidermal keratinocytes. Pampas grass ( Miscanthus sinensis var. purpurascens ) Concentrating the hexane (n-hexane) extract of flowers to obtain solid concrete (concrete) (step 1); and
Dissolving the solid concrete of step 2 in ethyl alcohol to remove the insoluble wax, and concentrating to obtain an essential oil in the form of a pampas grass flower absolute (step 2);

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