KR20220046031A - Composition comprising fraxinus rhynchophylla extract for antidepressant and antistress - Google Patents

Abstract

The present invention relates to an antidepressant and antistress composition containing a Fraxinus lanuginose leaf extract. More specifically, the present invention relates to a natural product-based antidepressant and antistress composition capable of exhibiting a stress reducing effect and a depression prevention and alleviation effect. The present invention can be taken or used for a long time while being able to exhibit effects such as antidepressant and antistress without the problem of side effects based on natural products.

Description

A composition for antidepressant and antistress that has an excellent antidepressant improving effect, including an ash leaf extract, and exhibits an antistress effect

The present invention relates to an antidepressant and antistress composition comprising an ash leaf extract. More specifically, it is a composition excellent in reducing depression and relieving stress by including an ash leaf extract as an active ingredient.

Stress is an adaptive response of the body in response to internal/external stressors or triggers, and medically, it is defined as a harmful stimulus that promotes the secretion of adrenocorticotropic hormone. The term 'stress' began to be used in 1936 when Canadian physiologist Dr. H. Selye published his "stress theory" in Nature.

Stimuli that cause stress are classified into physical, psychological, and physiological stimuli. Physical stimulation refers to temperature and ultraviolet rays that exist in nature, psychological stimulation refers to mental pain, anger, anxiety, tension, etc., and physiological stimulation refers to bacteria, viruses, and allergens.

In particular, when symptoms such as depression, anxiety, insomnia, and loss of appetite are repeated due to psychological stimulation, diseases such as depression, anxiety, and sleep disorder are caused.

Depression is a disease that causes a decrease in daily functioning by causing various cognitive, mental, or physical symptoms with a decrease in motivation and depression as the main symptoms. , prevalence, and suicide rate are also highly correlated.

Anxiety is a psychological disease that shows irritability, tension, and anxiety that are persistent and not recoverable over a period of usually 6 months or more.

Sleep disorders experience psychotic symptoms such as ego disintegration, hallucinations, and delusions, and as a result of a sleep deprivation experiment on rats, general weakness, skin disease, weight loss, increased energy consumption, and a drop in body temperature were observed. It has been reported to lead to death in severe cases (Dugovic et al., 1999 High corticosterone levels in prenatally stressed rats predict persistent paradoxical sleep alterations J. Neurosci. 19(19); 8656-8664).

When subjected to excessive mental stress, hormone secretion into the blood occurs through the circulation system leading to the hypothalamus-pituitary-adrenal axis (HPA axis).

Corticotropin releasing factor (CRF) is produced in the hypothalamus of the brain, which binds to the CRF receptor expressed in the pituitary gland and releases adrenocorticotropic hormone (ACTH). ACTH arrives at the adrenal gland through blood and lymph nodes and promotes the secretion of glucocorticoids such as cortisol, and the glucocorticoids secreted into the blood are delivered to each organ of the body.

Cortisol is a stress hormone among steroid hormones that prepares the human body to respond to mental stress by making muscles tense and sensitive to sensory organs.

On the other hand, the continuous secretion of cortisol hormone due to repetitive mental stress raises its blood level, keeps the body in a tense state, and interferes with deep sleep, leading to sleep disturbance.

This again causes mental stress, and the vicious cycle of depressing symptoms continues. When the cortisol secretion control function is lost due to extreme mental stress, the cortisol secretion is excessively secreted, causing brain atrophy and damage throughout the nervous system, leading to serious depression and suicide possibility.

In the case of a mouse overexpressing the CRF gene, the behavior of anxiety and depression is increased and the search behavior is decreased. It is reported that it may be implicated in emotional disorders and cause Cushing's syndrome.

It also shows physical changes such as excessive fat accumulation, muscle atrophy, thin skin and hair loss, and increases plasma levels of adrenocorticotropic hormone (ACTH) and cortisol. It is reported that these symptoms are alleviated by administration of a CRF antagonist (Stenzel-poore et al., 1994).

On the other hand, with regard to the development of anti-stress drugs, the market for dietary supplements for initial stress recovery is St. John's wort (Chilli pepper: improving depression and anxiety disorders) and Valerian (Valer: sedative, sleep improvement) have led, but the recent rapid expansion of the anti-stress market requires the development of new anti-stress substances.

However, only a small number of corresponding products, such as GABA, theanine, and phosphatidyl serine, have been proposed, and newly launched products are also being developed by mixing one or more of the above five types of anti-stress substances.

Depression drugs are drugs that induce a therapeutic effect through a temporary mood-altering effect by inhibiting the reuptake of serotonin secreted from nerve cell terminals into presynaptic cells, thereby increasing blood serotonin concentration and activity. There are approved and marketed prozac and celexa.

However, in the case of these serotonin reuptake inhibitors, only about half of the patients show symptom improvement, and a period of at least 4 months or longer is required, and depending on the patient, there is a problem in that the drug must be continuously taken for 2 to 3 years.

In addition, in the case of the serotonin reuptake inhibitor, when the drug is stopped, most of the symptoms recur within 6 to 12 months, and the side effects are also known to be serious.

As antianxiety drugs, benzodiazepines, such as diazepam, lorazepam, clonazepam, and alprazolam, are mainly used. Drugs from the imidazopyridine class are also used to treat short-term insomnia caused by anxiety.

However, drugs used for anxiety disorders and psychiatric disorders act directly on GABA (gammaaminobutyric acid) receptors in the central nervous system to suppress the central nervous system, resulting in excessive sedation and strengthening of central inhibitory effects such as depression, muscle relaxation, and hypnosis. may appear

In addition, drugs used for anxiety disorders and mental disorders contain risks such as fear of drug abuse by making them psychologically and physically dependent.

Accordingly, there is a need to develop a product that can solve the side effects of drugs used for the treatment of conventional anxiety disorders and mental disorders, and is made of natural substances that are harmful to the human body even when taken for a long time.

KR 10-2018-0067685 A1

An object of the present invention relates to an antidepressant and antistress composition comprising an ash leaf extract.

Another object of the present invention is to provide a composition for antidepressant and antistress based on natural products that can exhibit a stress reduction effect and depression prevention and improvement effect while including an ash leaf extract.

It is an object of the present invention to provide a composition that can be taken or used for a long time while being able to exhibit effects such as antidepressant and antistress without any side effects based on natural products.

In order to achieve the above object, a composition for antioxidant, antidepressant and antistress according to an embodiment of the present invention includes an ash leaf extract.

The ash leaf extract may be extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols, and mixtures thereof.

The composition may further include a red pepper leaf extract.

The composition may further comprise an extract of Panaxillarica.

The composition may further comprise a tea extract.

The functional food composition according to another embodiment of the present invention may include the antidepressant and antistress composition.

The pharmaceutical composition according to another embodiment of the present invention may include the antidepressant and antistress composition.

Hereinafter, the present invention will be described in more detail.

Throughout this specification, 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.

Throughout this specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Throughout this specification, the term "combination of these" included in the expression of the Markush form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Markush form, and the components It is meant to include one or more selected from the group consisting of.

Throughout this specification, the term "prevention" refers to any action that suppresses or delays the onset of arthritis by administration of the composition, and "treatment" or "improvement" means that the symptoms are improved or beneficial by administration of the composition Any action that changes

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

"Antioxidant" as used in the present invention refers to exhibiting the effect of reducing active oxygen and inhibiting oxidation or aging of cells.

Depression as used in the present invention, also called depressive disorder, refers to a disease that causes a decrease in daily functioning by causing various cognitive, mental, or physical symptoms with a decrease in motivation and depression as the main symptoms. On the other hand, "anti-depression" as used in the present invention refers to the effect of being able to prevent and improve gastric depression or depressive disorder, and to improve motivation and depression.

The definition of stress in the present invention is slightly different for each scholar, but stress refers to a detrimental response or change to an organism caused by an external stimulus. In general, when the stimulus on the living body exceeds a certain level, it acts invasively. At this time, the organism responds to a certain threat regardless of the type of stimulus, such as tension headache, migraine, high blood pressure, indigestion, fatigue, pain, It causes hair growth and roughness of the skin, and if it continues chronically, it results in a nonspecific general adaptive syndrome, such as various neurosis and gastric ulcer, which is defined as stress (Selye, 1958, Longmans Green). and Co.). In particular, various diseases such as mental disorders such as depression, digestive disorders such as neurosis and duodenal ulcer, endocrine disorders such as stress hyperthyroidism, skin disorders such as stress spots or acne, and cardiovascular disorders such as arrhythmias or angina pectoris It has been reported that the disease is caused by stress.

"Anti-stress" as used in the present invention refers to the effect of preventing or ameliorating the aforementioned stress or diseases caused by stress.

The composition for antioxidant, antidepressant and antistress according to an embodiment of the present invention comprises an ash leaf extract.

The ash tree leaf is the most important element of the present invention, and refers to the leaf of a plant commonly called ash tree.

Ash trees are usually bush-shaped and are commonly grown in mountainous areas. The leaves are opposite, and it is a right-sided bi-lobed with 1 rhizome. Small leaves are 5-7, ovate or lanceolate, with saw teeth on the edge and hairs on the veins on the back side. Flowers bloom in May and are bivalent, but there are also bisexual flowers, and they hang on the panicle grown from a new branch. Male flowers have 2 stamens and calyx pieces, and female flowers have 2-4 calyx pieces, stamens, and pistils. The fruit is a citrus fruit and is 2 to 4 centimeters long.

When the ash leaf extract is included, along with antioxidant activity, it can exhibit the effect of preventing and alleviating stress and preventing and improving depression. In addition, through the prevention and alleviation of stress, it is possible to prevent and improve various diseases that may be caused by stress.

In the antidepressant and antistress composition, the ash leaf extract may be extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols, and mixtures thereof.

Preferably, the solvent may be any one selected from the group consisting of water, ethanol, and mixtures thereof. In the case of the solvent, the yield for the active ingredient exhibiting antioxidant, anti-stress and anti-depressant effects can be maintained at a high level, and thus a more excellent effect can be exhibited compared to a relatively small content.

The antidepressant and antistress composition may further include a red pepper leaf extract.

The red pepper leaf refers to a leaf of hot pepper.

The anti-depressant and anti-stress composition may further include an extract of Beomtail grass.

The pan-tailed grass grows in sunny places in mountain valleys. 30 to 80 cm in height. The rhizome is short and thick, and there are many fine roots. The leaf attached to the root is alternate phyllotaxis, the petiole is long, broad egg-shaped, gradually narrows and has a sharp tip, and the bottom is in the shape of a heart. The edge of the leaf is flat and the back side is white. The leaves are 5 to 10 cm long and 3 to 7 cm wide. The leaves on the stem are similar to this, but the petiole is short and the leaves are small. The sheath is membranous (膜質: translucent like thin paper). Flowers bloom in June or July in light pink or white, and hang on the inflorescence. There are no petals and the sepals are divided into 5 pieces. There are 8 stamens, longer than the calyx, the anthers are light reddish purple, and there is a small gland [腺] at the bottom of the stamens. Fruits are aqueous and ripen in September or October, are wrapped in calyx, and have 3 ridges. The young leaves and stems are edible, and the rhizomes are used to relieve heat, control competition, and eliminate inflammation of boils. It is distributed in Korea, northeastern China, Heilong River, and Ussuri River.

The antidepressant and antistress composition may further include a chazugi extract.

The chazugi (Perilla frutescens var. acuta) has a straight stem, a height of 20 to 80 cm, a square cross section, a purple color, and a fragrance. The leaves are opposite, broad egg-shaped, pointed at the tip, rounded at the bottom, and serrated at the edge. There are hairs on both sides of the leaves, long hairs on the veins on the back side, and long petioles. Flowers bloom in August or September in light purple and run in raceme at the ends of stems and branches. The calyx has hairs and is split into two, and the upper part of the split pieces is split into three again, and the lower piece is split into two again. The corolla is short, cylindrical, and the tip is lip-shaped, and the lower lip is slightly longer than the upper lip. There are 4 stamens, and 2 of them are long. The fruit is a branch (separate: each fruit divided from the fissionaceae), is round, has a diameter of 1.5mm, and is contained in a calyx.

When the red pepper leaf, pantail grass and chazugi extract are additionally included, it may exhibit synergistic effects on antioxidants, antidepressants and antistress.

Preferably, the antidepressant and antistress composition comprises an ash leaf extract, and 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the ash leaf extract; It may be one comprising 5 to 50 parts by weight of sagebrush and 0.1 to 20 parts by weight of chazugi extract.

According to the above range, the antioxidant, antidepressant and anti-stress effects may be significantly increased due to the synergistic effect by the interaction of each extract. Therefore, it can produce a high effect with a smaller content, and there is no problem of cytotoxicity and other side effects even when taken for a long time.

More preferably, the antidepressant and antistress composition comprises an ash leaf extract, and 0.1 to 20 parts by weight of a red pepper leaf extract based on 100 parts by weight of the ash leaf extract; 5 to 50 parts by weight of sagebrush; 0.1 to 20 parts by weight of chazugi extract; 0.1 to 10 parts by weight of Lamiaceae extract; It may include 0.1 to 10 parts by weight of red snow tea extract and 0.1 to 10 parts by weight of Cheonmundong extract.

The Lamiaceae is a perennial herb commonly grown in mountains and fields across the country, and is also distributed in China and Japan. There is a rhizome. Stems are reddish, hairy, and 20-60cm tall. The leaves are opposite, ovate or ovate oval, with a flat edge or some serrations. Flowers are densely hung on the inflorescence at the tip of the stem in May to July, and are purple, pink, white, and lip-shaped. The calyx is lip-shaped, divided into 5 parts. The lower lip of the corolla is divided into three parts. 4, 2 stamens are long. The fruit is a soybean, split into 4, and ripens to yellowish brown.

The snow tea (Snow Tea, 紅雪茶) refers to a dried moss plant that grows wild in an old juniper tree in an alpine region.

The Cheonmundong grows on the seashore, and its rhizomes are short and many cylindrical roots with pointed ends spread in all directions. The lower part of the stem has egg-shaped scales. Stems are green, reaching 1 to 2m in length, tendrils, and leaf-like branches run in groups of 1 to 3 and bend like a bow. Flowers bloom in May or June, hang 1 to 3 in leaf axils, light yellow, and small flower stalks have joints in the center and are similar to the length of petals. Inflorescences are yellowish-white in color and have a long oval shape. There are 6 petals and 6 stamens, and 3 styles. The fruit is a berry, round, about 6mm in diameter, matured in white, and contains 1 black seed.

When the Lamiaceae extract, red snow tea extract and Cheonmundong extract are additionally included within the above range, the interaction of each active ingredient not only exhibits an additional synergistic effect on antioxidant, antidepressant and antistress, but also significantly increases flavor and palatability. can pay Accordingly, it is possible to widely distribute food and fragrance compositions with excellent palatability.

The functional food composition according to another embodiment of the present invention may include the antidepressant and antistress composition.

At this time, the amount of the extract in the food or beverage may be added in an amount of about 0.01 to 15% by weight of the total food weight, and the health drink composition may be added in a ratio of about 0.01 to 10g based on 100 ml, but is not limited thereto. .

When the food composition of the present invention is a beverage composition, there is no particular limitation on the other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and it may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages. can Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) may be used, but are not limited thereto. The proportion of the natural carbohydrate may be generally in the range of about 1 to 20 g per 100 ml of the composition of the present invention, but may not be limited thereto.

In addition to the above, the composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts. salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc., but may not be limited thereto.

In addition, the composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages, and these components may be used independently or in combination. The proportion of these additives is not critical, but may be selected from about 0.1 to about 20 parts by weight per 100 parts by weight of the composition of the present invention, but is not limited thereto.

Functional food as referred to in the present invention is a food manufactured and processed using a specific ingredient as a raw material for the purpose of health supplement or by extracting, concentrating, refining, or mixing a specific ingredient contained in a food raw material, including health supplement food, In addition, it is a food that is designed and processed to sufficiently exert its biological control functions, such as biological defense, regulation of biological rhythm, and prevention and recovery of diseases, and has functions related to disease prevention and recovery from diseases. to include everything.

The perfume composition according to another embodiment of the present invention may include the antidepressant and antistress composition.

When exemplifying the embodiment of the invention for the perfume, the composition of the present invention may be implemented in the form of a perfume, a diffuser, a scented candle, a massage oil, a body oil, a fragrance lamp, an aroma stick, a spray, a soap (eg, beauty soap) and a bath agent. can

In one embodiment, the composition of the present invention may be embodied as a perfume, a bath agent or a perfume formulation, in which case the perfume composition of the present invention may further include components commonly added to perfume manufacturing in addition to the mixed essential oil. These ingredients include fragrances, preservatives, stabilizers, colorants, antibacterial agents, deodorants, and disinfectants.

The pharmaceutical composition according to another embodiment of the present invention may include the antidepressant and antistress composition.

The present invention provides a natural product-based antidepressant and anti-stress composition that can exhibit a stress reduction effect and depression prevention and improvement effect while including an ash leaf extract.

The present invention provides a composition that can be taken or used for a long time while being able to exhibit effects such as antidepressant and antistress without the problem of side effects based on natural products.

1 is an experimental result on the antioxidant effect of an ash leaf extract according to an embodiment of the present invention.
2 is an experimental result on the antioxidant effect of ash leaf extract according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of plant extract]

1. Preparation of Ash Leaf Extract

에서 증발하였다. 그리고 남은 시료는 동결건조하였다. 본 실험에 사용한 물푸레나무 잎 추출물(BLE)은 물푸레나무 잎 원재료에서의 수득률은 10.5%로 추출하였다.3 g of freeze-dried ash leaves were put into 1 mg in a 15 ml tube, and 10 ml of 70% ethanol was added again. The sample was put in a rounded mixed machine for 18 hours, mixed and extracted. After extraction, only the supernatant was collected after centrifugation at 3000 rpm and 10 minutes. Ethanol contained in the supernatant collected in this way was 40

evaporated from And the remaining sample was freeze-dried. The ash leaf extract (BLE) used in this experiment was extracted with a yield of 10.5% from the raw ash leaf extract.

2. Preparation of other plant extracts

Using the same method as for the preparation of the ash leaf extract, red pepper leaf extract (AE), sagebrush extract (BE), chazugi extract (CE), Lamiaceae extract (DE), red snow tea extract (EE) and cheonmundong extract ( FE) was prepared.

3. Preparation of Complex Extracts

In order to confirm the synergistic effect of mixing each extract, each extract was mixed according to the composition shown in Table 1 below to prepare a complex extract.

BX1 BX2 BX3 BX4 BX5 BX6 BX7 BX8 BX9 BLE 100 100 100 100 100 100 100 100 100 AE 0.01 0.1 10 20 30 10 10 10 10 BE One 5 30 50 100 30 30 30 30 CE 0.01 0.1 10 20 30 10 10 10 10 DE - - - - - 0.01 0.1 10 20 EE - - - - - 0.01 0.1 10 20 FE - - - - - 0.01 0.1 10 20

(Unit: parts by weight)

[Experimental example: antioxidant, antidepressant, anti-stress test]

1. Animal Test Methods and Animal Groups

(1) Animal testing method

, 습도는 55-60 %를 유지하여 사육하였다. 주간-야간 주기는 12시간으로 조절하였다. 일반배합사료(PMI Nutrition LLC. Manufactured in the USA. 5L79) 및 음수는 제한을 두지 않았고 일주일간 사육실 환경에 적응기를 가진 후 실험을 실시하였다. 실험은 모두 주간에 실시하였다.Four-week-old Spraque-Dawley rats with a body weight of 100 to 120 g (Korea Central Animal, Gyeonggi-do, Korea) were purchased from 30 females and the room temperature was 20±2.

, the humidity was maintained at 55-60% for breeding. The day-night cycle was adjusted to 12 hours. There were no restrictions on general formula feed (PMI Nutrition LLC. Manufactured in the USA. 5L79) and drinking water, and the experiment was conducted after a period of adaptation to the breeding room environment for a week. All experiments were conducted during the day.

(2) group of animals

5 animals per group were divided into 2 groups. In group 1, the control group was administered with water orally, and group 2 was orally administered with ash leaf extract (BLE) 15mg/150g (rat weigh).

2. Antioxidant test

(1) Measurement of DPPH radical scavenging activity

DPPH (2,2-diphenyl-1-picrylhydrazyl)

The hydrazyl of DPPH (Diphenylpicryl hydrazyl) used in this experiment has a property of easily accepting hydrogen atoms because nitrogen atoms are in an unstable state.

에서 30분 동안 incubation시켰다. 이후 spectrophotometer를 이용하여 517 nm에서 흡광도를 측정하였다.The ash leaf extract was prepared by adding 1.9 ml of 0.2 mM DPPH solution (99.5% ethanol) to 0.1 ml (control: 99.5% ethanol) of 5 concentrations of 0.05, 0.1, 0.2, 0.5, and 1 mg/ml. added. After shaking for 10 seconds with a vortex mixer, 37

incubated for 30 min. Then, the absorbance was measured at 517 nm using a spectrophotometer.

As a positive control drug, L-ascorbic acid was prepared at 5 concentrations of 0.05, 0.1, 0.5, and 1 mg/ml (99.5% ethanol) and measured. The antioxidant activity of each sample was expressed as % Antioxidant activity (electron donating ability) against DPPH.

The experimental results are shown in FIG. 1 .

1, the ash leaf extract was 11.6% at 0.05 mg/ml, 22.8% at 0.1 mg/ml, 37.7% at 0.2 mg/ml, 52.8% at 0.5 mg/ml, and 60.8% at 1 mg/ml, L-ascorbic acid showed antioxidant results of 38.3% at 0.05 mg/ml, 46.5% at 0.1 mg/ml, 58.2% at 0.2 mg/ml, 83.5% at 0.5 mg/ml and 89.5% at 1 mg/ml.

As a result, compared to the positive control, which is known to have antioxidant effects, the ash leaf extract showed about twice the antioxidant results.

(2) Xanthine oxidase inhibitory activity measurement

에서 5분간 반응시킨 후 1 N HCl 1 ml를 가하여 반응을 종료시킨 다음, 반응액 중에 생성된 uric acid를 292 nm에서 흡광도를 측정하였다. Xanthin oxidase저해 활성은 시료용액의 첨가군과 무첨가군의 흡광도 감소율로 나타내었다.To 0.1 ml of sample solution and 0.6 ml of 0.1 M potassium phosphate buffer of pH 7.5, 0.2 ml of substrate solution in which 2 mM xanthine was dissolved was added, and 0.2 unit/ml xanthine oxidase 0.1ml was added to 37

After reacting for 5 minutes, 1 ml of 1 N HCl was added to terminate the reaction, and the absorbance of uric acid generated in the reaction solution was measured at 292 nm. Xanthin oxidase inhibitory activity was expressed as a decrease in absorbance in the group with and without the sample solution.

Xanthine oxidase is an enzyme that acts as a rate-limiting enzyme in the terminal oxidation of all purines and as a source of oxidizing agents such as superoxide radicals and hydrogenperoxide. The inhibition of superoxide anion by xanthine/xanthine oxidase enzymes is shown by the scavenging of superoxide anion and inhibition of xanthine oxidase enzymes, and has biological significance through inhibition of free radical production. The superoxide radical scavenging effect of 50 μM allopurinol as a positive control was compared.

The result is shown in FIG. 2 .

Referring to FIG. 2, it will show a scavenging effect that is about 50% higher at 1 mg/ml than 50 μM of allopurinol 50 μM, which is a positive control, indicating that there is an excellent antioxidant effect.

3. Antidepressant behavior test (Forced Swimming Test, Forced Swimming Test, FST)

After arriving at the kennel, the experimental animals were acclimatized to the kennel environment for 14 days, and both groups were acclimatized by conducting a forced swimming test for 15 minutes to acclimatise to the water. was taken After missing 5 minutes of the forced swimming test, it appears that they are actively trying to escape at the beginning, but they soon fall into a sleep fear and maintain an immobility according to the degree of depression, so immobility time was measured. The experiment was repeated 3 times.

Meanwhile, the results are shown in Table 2 below.

1st 2nd 3rd Control 168.5 175.6 188.9 BLE 171.5 113.3 120.8

As a result of analyzing the results of the antidepressant behavior experiment through three repeated experiments, it was confirmed that a significant reduction effect compared to the control group was exhibited. That is, it was confirmed that the immobility time was shortened in the group to which the ash leaf extract was administered.

4. Stress improvement effect test

Ten men and women who consented to this experiment were randomly classified into a control group taking a placebo and an experimental group taking ash leaf extract (BLE), each of which was arbitrarily divided into 5 people each, and consumed 400 mg each in the form of a tea drink for 4 weeks. .

In order to examine the psychological health improvement effect, PWI (Psychosocial Well-being Index), a social and psychological health measurement test, was conducted before and after intake. For objective comparison, the value of the control group was fixed at 10, the index was converted on a 10-point scale, and the index was evaluated as an index of 1 to 10, and the results are shown in Table 3 below.

In the following index, the lower the number, the better the stress improvement effect.

placebo BLE 10 8.8

(Unit: Index)

Referring to Table 3, it can be seen that the continuous use of the ash leaf extract according to the present invention can help relieve stress. In particular, it can be seen that self-confidence and mental and physical stability can be increased according to the PWI index. Therefore, when the ash leaf extract is continuously used, it is possible to relieve stress, and it is possible to exhibit the prevention and improvement effect on diseases caused by stress.

4. Antioxidant, antidepressant, anti-stress test for complex extracts

Antioxidant (AO), antidepressant (AP) and antistress (AS) effects on the complex extracts BX1 to BX9 were evaluated in the same manner as above. In addition, in order to check whether a synergistic effect appears compared to the ash leaf extract (BLE), BLE was set as a fixed value, and the activity against BX1 to BX9 was compared.

Therefore, for objective comparison, the following evaluation indices fixed the BLE result to 1 in the case of AO and AP, and converted the values of BX1 to BX9 into indices of 1 to 10 compared to BLE.

AO and AP in the above index, the higher the number, the better the effect. On the other hand, indices calculated as 1 and less than 1 in AO and AP are also indicated as 1. Therefore, the index indicated by 1 in AO and AP does not have a synergistic effect, or the effect is inferior to that of using BLE alone.

In addition, in the case of AS, the result of BLE was fixed to 10, and the values of BX1 to BX9 were compared with BLE and converted into an exponent of 1 to 10. As for AS in the above index, the lower the number, the better the effect. On the other hand, the index calculated to exceed 10 by 1 in AS is also denoted as 10. Therefore, the index represented by 10 in AS does not have a synergistic effect, or its effect is inferior to that of using BLE alone.

The results are shown in Table 4 below.

BLE BX1 BX2 BX3 BX4 BX5 BX6 BX7 BX8 BX9 AO One One 3.1 4.2 4.2 1.4 3.3 4.6 5.4 2.3 AP One One 3.4 3.1 3.9 1.1 2.8 4.7 5.2 1.1 AS 10 10 8.7 8.5 8.8 9.8 8.8 6.1 6.7 8.3

(Unit: Index)

Referring to Table 3, in the case of BX1, there was no synergistic effect on the complex extract, and in the case of BX5, there was no significant synergistic effect while a relative increase of other extracts other than BLE, and it was confirmed that some indexes were lower than BLE. .

However, as in BX2 to BX4, according to the embodiment in which the red pepper leaf extract, the oleander extract and the chazugi extract are included in a specific content range, the ash leaf extract is used alone as a mutual combination of the active ingredients included in each extract. It can be seen that there is a synergistic effect compared to the case. In addition, in the case of B3, it was found that antioxidant activity, antidepressant activity and antistress activity were significantly improved.

On the other hand, in the case of BX and BX8, it can be confirmed that an additional synergistic effect can be exhibited. Therefore, it can be confirmed that, in the case of BX7 and BX8, very excellent antioxidant activity, antidepressant activity and anti-stress activity can be exhibited with a smaller content, and continuous administration or use for a long period of time is possible.

5. palatability test

The ash leaf extract (BLE) and BX1 to BX9 were processed into tea form, and each taster was asked to evaluate the preference based on flavor and taste. The tasters were asked to evaluate the palatability of BX1 to BX9 on an index of 1 to 10 by comparing the taste to the ash leaf extract by 10 people who evaluated the gastric stress relieving effect. The evaluated result values are taken as the average, and are shown in Table 4 below.

The following evaluation values were rounded off with a decimal point, and the higher the number, the better the palatability.

BLE BX1 BX2 BX3 BX4 BX5 BX6 BX7 BX8 BX9 flavor 5 5 4 5 4 3 5 8 9 6 taste 5 4 4 5 4 2 5 8 8 6 Comprehensive symbol map 5 4 4 5 4 3 5 8 9 6

Referring to Table 5, it can be seen that in the case of BX1 to BX5, a similar or lower degree of palatability compared to BLE appears. On the other hand, in the case of BX7 to BX9, it can be seen that the effect of improving flavor and taste appears, and in particular, the degree of improvement in flavor and taste of BX7 and BX8 is excellent.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (7)

Contains Ash Leaf Extract
Compositions for antidepressant and antistress. The method of claim 1,
The ash leaf extract is extracted using an extraction solvent selected from the group consisting of water, C1 to C6 lower alcohols and mixtures thereof.
A composition for antidepressant and antistress. According to claim 1,
The composition further comprises a red pepper leaf extract
Compositions for antidepressant and antistress. According to claim 1,
The composition further comprises an extract
Compositions for antidepressant and antistress. According to claim 1,
That the composition further comprises a chazugi extract
Compositions for antidepressant and antistress. A functional food composition comprising the antidepressant and antistress composition according to any one of claims 1 to 5. A pharmaceutical composition comprising the composition for antidepressant and antistress according to any one of claims 1 to 5.

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