KR20220039354A - Cooling cosmetic composition for preventing of skin heat aging and improving of pores - Google Patents

Abstract

The present invention relates to a cooling cosmetic composition which comprises a mushroom fermented product and sugar alcohols and to a preparation method thereof. The cooling cosmetic composition imparts a continuous cooling effect through an endothermic reaction of absorbing heat on a skin surface by mixing the mushroom fermented product and the sugar alcohols at a certain mixing ratio, and has the advantages of skin cooling persistence, pore size reduction, the alleviation and prevention of skin wrinkle due to heat, low irritation to the skin, the alleviation of skin density, and skin soothing, and thus solves the problems of lack of persistence, undesirable irritation, peculiar smell, and bitter taste that an existing cooling agent has.

Description

Cooling cosmetic composition for preventing of skin heat aging and improving of pores

The present invention relates to a cosmetic composition for preventing, preventing, and reducing skin pore area, and a method for manufacturing the same, comprising a mushroom fermented product and sugar alcohols.

An agent that gives the human skin, mouth, nose and throat an effect such as a refreshing feeling (cool and refreshing feeling) and a cool feeling (chilly and refreshing feeling) is called a cooling agent. do. The cooling agent that gives such a cooling effect has been widely used in toothpaste, chewing gum or confectionery such as candy, tobacco, cataplasma, and cosmetics.

Major fragrance substances that give a cooling sensation or cooling sensation include l-menthol (L-menthol), l-menthyl glucoside, and esters of 3-hydroxymethyl-p-mentane. Menthol derivatives such as 3-substituted-p-menthane exist as compounds proposed in . Among them, l-menthol is most widely used as a fragrance material.

In addition, as compounds having a cooling effect, various compounds such as peppermint oil, methyldiisopropylpropionamide, ethylmentane carboxamide and peppermint flower/leaf/stem oil have been proposed and used in practice. However, the above fragrance substance or oil component lacks the durability of the cooling effect, and if the amount used to increase the cooling effect is increased, the cooling effect is enhanced, but it has a disadvantage that is accompanied by a bad taste, stinging eyes, or skin irritation.

In addition, although the cooling agent has a cooling effect to some extent, the cooling effect is insufficient, the feeling of satisfaction in terms of durability is low, and the actual effect on the skin is insignificant, so improvement is urgently needed.

Accordingly, the present inventors have intensively researched and endeavored to develop a cooling cosmetic composition that does not cause undesirable irritation, specific odor or bitter taste to the skin. As a result, it was confirmed that the cooling cosmetic composition containing the mushroom fermented product and sugar alcohol was significantly superior in the prevention and prevention of skin heat aging, the reduction of skin pore area, and the durability of a feeling of refreshment or cooling.

Accordingly, an object of the present invention is to provide a cosmetic composition for preventing and preventing skin heat aging and reducing pore area, comprising a mushroom fermented product and sugar alcohols.

Another object of the present invention is to provide a method for preparing a cooling cosmetic composition comprising a manufacturing step of preparing a mushroom fermented product and a mixing step of mixing the mushroom fermented product and sugar alcohols.

The present inventors have made intensive research efforts to develop a cooling cosmetic composition that does not cause undesirable irritation, specific odor, or bitter taste to the skin. As a result, it was found that the cooling cosmetic composition containing the mushroom fermented product and sugar alcohols was significantly superior in preventing and preventing skin heat aging, reducing skin pore area, and maintaining a feeling of refreshment or cooling sensation.

Hereinafter, the present invention will be described in more detail.

An example of the present invention relates to a cooling cosmetic composition comprising a mushroom fermented product and sugar alcohols.

In the present invention, the weight ratio of the mushroom fermented product and the sugar alcohols may be 1:40 to 60, 1:40 to 55, 1:40 to 50, 1:40 to 45, for example, 1:40, The present invention is not limited thereto.

In the present invention, the mushroom is a multi-faceted mushroom ( Albatrellus confluens (Alb. & Schwein.) Kotl. & Pouz.), a woolly shield mushroom ( Albatrellus ovinus (Schaeff.) Kotl. & Pouz.), Albatrellus dispansus ( Albatrellus dispansus ( Lloyd) Canf. & Gilb.), green shield mushroom ( Albatrellus caeruleoporus (Peck) Pouz.), gray-blue shield mushroom ( Albatrellus yasudae (Lloyd) Pouzar), long-legged shield mushroom ( Albatrellus pes- caprae ), purple cotton mushroom ( Albatrellus cristatus ) and coarse hairy shield mushroom ( Albatrellus ellisii ) may include one or more selected from the group consisting of, for example, it may include a multifaceted mushroom, but is not limited thereto.

In the present invention, the mushroom fermented product may be an extract or a fraction thereof fermented by an enzyme or yeast, for example, an extract fermented by Saccharomyces cerevisiae or a fraction thereof. , but is not limited thereto.

In the present invention, the mushroom fermented product is 0.0500 to 2.0000 wt%, 0.0500 to 1.8000 wt%, 0.0500 to 1.5000 wt%, 0.0500 to 1.3000 wt%, 0.0500 to 1.0000 wt%, 0.0500 to 0.8000 wt%, 0.0500 to the total weight of the cosmetic composition to 0.5000 wt%, 0.0500 to 0.3000%, 0.0500 to 0.1000 wt%, for example, 0.0500 wt% may be included, but is not limited thereto.

In the present invention, the sugar alcohols may be one or more selected from the group consisting of erythritol, xylitol, trehalose, sorbitol and maltitol, and may be, for example, erythritol, xylitol or trehalose. For example, it may be erythritol, but is not limited thereto.

In the present invention, the sugar alcohol is 2.0000 to 10.5000% by weight, 2.0000 to 8.0000% by weight, 2.0000 to 5.0000% by weight, 2.0000 to 3.0000% by weight, for example, 2.0000% by weight, based on the total weight of the cosmetic composition. The present invention is not limited thereto.

In the present invention, the cooling cosmetic composition may further include at least one selected from the group consisting of a moisturizing agent, a viscosity increasing agent, a skin soothing agent, an emulsifier, a conditioning agent, a sequestering agent, and an antioxidant, but is not limited thereto. .

In the present invention, the moisturizing agent is propanediol, methylpropanediol, glycerin, dipropylene glycol, butylene glycol, PEG-8, propylene glycol, diglycerin, polyglycerin-3 and glycerose-26 (Glycereth-26) may be one or more selected from the group consisting of, but is not limited thereto.

In the present invention, the viscosity increasing agent is xanthan gum, carob gum, guar gum, acrylate/C10-30 alkyl acrylate crosspolymer, ammonium acryloyldimethyltaurate/Vpicopolymer, polyacrylate crosspolymer-6, It may be at least one selected from the group consisting of hydroxyethyl cellulose, hydroxypropylmethyl cellulose and ethyl cellulose, but is not limited thereto.

In the present invention, the skin soothing agent may be one or more selected from the group consisting of allantoin, panthenol, dipotassium glycyrrhizate and Centella asiatica extract, but is not limited thereto.

In the present invention, the emulsifier is PEG-40 hydrogenated castor oil, polyglyceryl-10 laurate, polyglyceryl-10 myristate, polyglyceryl-10 diisostearate, polyglyceryl-10 oleate, poly It may be at least one selected from the group consisting of glyceryl-2 caprate and polyglyceryl-4 caprate, but is not limited thereto.

In the present invention, the conditioning agent may be at least one selected from the group consisting of sodium hyaluronate, biosaccharide gum-1 caffeine, acetylglucosamine, fructose and fructooligosaccharide, but is not limited thereto. .

In the present invention, the sequestering agent may be at least one selected from the group consisting of disodium EDTA and sodium phytate, but is not limited thereto.

In the present invention, the antioxidant may be one or more selected from the group consisting of tocopherol, tocopheryl acetate, ascorbic acid, tocopheryl linoleate/oleate and sodium ascorbyl phosphate, but is not limited thereto.

Another embodiment of the present invention relates to a method for preparing a cooling cosmetic composition comprising the steps of:

Mushroom fermented product manufacturing step of preparing the mushroom fermented product; and

A sugar alcohol mixing step of mixing the mushroom fermented product and sugar alcohols.

In the present invention, the step of preparing a fermented mushroom may include the following steps:

A powder solution preparation step of mixing and stirring by adding a solvent to the mushroom powder;

An extraction step of extracting the powder liquid into a powder extract or powder; and

A step of preparing a powdered extract or a fermentation extract in which the powder is fermented with an enzyme or yeast.

In the present invention, the powder liquid preparation step may be to add one or more solvents selected from the group consisting of distilled water and ethanol, but is not limited thereto.

When distilled water is used, distilled water is added by weight 10 to 30 times, 10 to 25 times, 10 to 20 times, 15 to 30, 15 to 25, 15 to 20, for example, 20 times the weight of distilled water powder liquid It may be to manufacture, but is not limited thereto.

When using ethanol, the ethanol is 5 to 15 times, 5 to 13 times, 5 to 11 times, 7 to 15 times, 7 to 13 times, 7 to 11 times, for example, 10 times the weight of mushroom powder by adding It may be to prepare an ethanol powder solution, but is not limited thereto.

In the present invention, the extraction step may be hot water extraction of the distilled water powder liquid into the powder extract liquid, but is not limited thereto.

The hot water extraction is 110 to 130 ° C, 110 to 125 ° C, 115 to 130 ° C, 115 to 125 ° C, 120 to 130 ° C, 120 to 125 ° C, for example, hot water extraction of the distilled water powder liquid at 121 ° C. However, the present invention is not limited thereto.

The hot water extraction may be hot water extraction of the distilled water powder solution for 10 to 20 minutes, 10 to 18 minutes, 10 to 16 minutes, 13 to 20 minutes, 13 to 18 minutes, 13 to 16 minutes, for example, 15 minutes. However, the present invention is not limited thereto.

In the present invention, the extraction step may be powder extraction of the ethanol powder solution, but is not limited thereto.

The powder extraction is 30 to 55 ° C, 30 to 50 ° C, 30 to 45 ° C, 35 to 55 ° C, 35 to 50 ° C, 35 to 45 ° C, 40 to 45 ° C, 40 to 45 ° C, for example, stirring the ethanol powder solution at 45 ° C. It may include, but is not limited to.

The powder extraction may include stirring the ethanol powder solution for 2 to 5 hours, 2 to 4 hours, for example, 3 hours, but is not limited thereto.

The powder extraction may include centrifuging the ethanol powder solution at 5000 to 7000 rpm, 5000 to 6500 rpm, for example, 6000 rpm, but is not limited thereto.

The powder extraction comprises centrifuging the ethanol powder solution for 10 to 30 minutes, 10 to 25 minutes, 10 to 20 minutes, 15 to 30 minutes, 15 to 25 minutes, 15 to 20 minutes, for example, 20 minutes. may be, but is not limited thereto.

Powder extraction in the present invention is 0.30 to 0.55 um, 0.30 to 0.50 um, 0.30 to 0.45 um, 0.35 to 0.55 um, 0.35 to 0.50 um, 0.35 to 0.45 um, 0.40 to 0.55 um, 0.40 to 0.50 um, ethanol powder solution , 0.40 to 0.45 um, for example, may include filtering with a 0.45 um filter, but is not limited thereto.

Powder extraction in the present invention may include, but is not limited to, extracting into powder by concentrating the ethanol powder solution.

In the present invention, the fermentation extract preparation step may be to mix the distilled water powder extract and ethanol powder, but is not limited thereto.

In the present invention, the fermentation extract preparation step may be an extract or a fraction thereof fermented by an enzyme or yeast, for example, an extract fermented by Saccharomyces cerevisiae or a fraction thereof. However, the present invention is not limited thereto.

The fermentation extract preparation step may be performed at 20 to 35 ℃, 20 to 30 ℃, 25 to 35 ℃, 25 to 35 ℃, for example, 30 ℃, but is not limited thereto.

The fermentation extract preparation step may be performed for 65 to 75 hours, 65 to 73 hours, 67 to 75 hours, 67 to 73 hours, 70 to 75 hours, 70 to 73 hours, for example, 72 hours. It is not limited.

In the present invention, the mushroom fermented product production step relates to a production method further comprising the following steps:

Sterilization step of sterilizing the fermentation extract; and

A obtaining step of isolating the supernatant of the sterilized fermentation extract to obtain a mushroom fermented product.

In the present invention, the sterilization step is 110 to 130 ℃ fermentation extract. 110 to 125 °C, 115 to 130 °C, 115 to 125 °C, 120 to 130 °C, 120 to 125 °C, for example, may include sterilization at 121 °C, but is not limited thereto.

The sterilization step may be sterilizing the fermentation extract for 10 to 20 minutes, 10 to 15 minutes, for example, 15 minutes, but is not limited thereto.

In the present invention, the obtaining step may be to obtain a mushroom fermented product by centrifuging the supernatant of the sterilized fermentation extract at 5000 to 7000 rpm, 5000 to 6500 rpm, for example, 6000 rpm, but is not limited thereto.

In the obtaining step, the supernatant of the sterilized fermentation extract is centrifuged for 10 to 30 minutes, 10 to 25 minutes, 10 to 20 minutes, 15 to 30 minutes, 15 to 25 minutes, 15 to 20 minutes, for example, 20 minutes. It may be to obtain a mushroom fermented product, but is not limited thereto.

In the obtaining step in the present invention, the supernatant of the sterilized fermentation extract is filtered with a filter with 0.01 to 0.5 um, 0.01 to 0.3 um, 0.1 to 0.5 um, 0.1 to 0.3 um, for example, 0.2 um to obtain a mushroom fermentation may be, but is not limited thereto.

In the present invention, the sugar alcohol mixing step is performed at 20 to 30 ° C., 20 to 28 ° C., 20 to 26 ° C., 23 to 30 ° C., 23 to 28 ° C., 23 to 26 ° C., for example 25 ° C. It may be cooling, but is not limited thereto.

In the present invention, the sugar alcohol mixing step comprises a weight ratio of the mushroom fermented product and sugar alcohols of 1:40 to 60, 1:40 to 55, 1:40 to 50, 1:40 to 45, for example, 1: 40 may be mixed, but is not limited thereto.

In the present invention, the mushroom is a multi-faceted mushroom ( Albatrellus confluens (Alb. & Schwein.) Kotl. & Pouz.), a woolly shield mushroom ( Albatrellus ovinus (Schaeff.) Kotl. & Pouz.), Albatrellus dispansus ( Albatrellus dispansus ( Lloyd) Canf. & Gilb.), green shield mushroom ( Albatrellus caeruleoporus (Peck) Pouz.), gray-blue shield mushroom ( Albatrellus yasudae (Lloyd) Pouzar), long-legged shield mushroom ( Albatrellus pes- caprae ), purple cotton mushroom ( Albatrellus cristatus ) and coarse hairy shield mushroom ( Albatrellus ellisii ) may include one or more selected from the group consisting of, for example, it may include a multifaceted mushroom, but is not limited thereto.

In the present invention, the sugar alcohols may be one or more selected from the group consisting of erythritol, xylitol, trehalose, sorbitol and maltitol, and may be, for example, erythritol, xylitol or trehalose. For example, it may be erythritol, but is not limited thereto.

In the present invention, the cooling cosmetic composition may further include at least one selected from the group consisting of a moisturizing agent, a viscosity increasing agent, a skin soothing agent, an emulsifier, a conditioning agent, a sequestering agent, and an antioxidant, but is not limited thereto. .

In the manufacturing method of the cooling cosmetic composition, content overlapping with the cooling cosmetic composition is omitted in consideration of the complexity of the present specification.

The present invention relates to a cooling cosmetic composition comprising a mushroom fermented product and sugar alcohols, and by mixing the mushroom fermented product and sugar alcohols at a predetermined mixing ratio, a continuous cooling effect is imparted through an endothermic reaction of absorbing heat to the skin surface It has the advantages of skin cooling lasting, pore size reduction, prevention of skin wrinkle improvement due to heat, low irritation to the skin, improvement of skin density and skin soothing. And it is expected to have an effect of resolving the problem of bitter taste.

1A is a photograph showing the cooling persistence according to time after application of Examples 1 and 2 according to an experimental example of the present invention.
1B is a graph showing the duration of cooling sensation according to time after application of Examples 1 and 2 according to an experimental example of the present invention.
1c is a graph showing the increase/decrease rate of the cooling duration with time after application of Examples 1 and 2 according to an experimental example of the present invention.
Figure 2a is a photograph showing the increase or decrease of the pore area before and after application of Example 2 according to an experimental example of the present invention.
Figure 2b is a graph showing the increase and decrease of the pore area before and after application of Example 2 according to an experimental example of the present invention.
2C is a graph showing the pore area increase/decrease rate before/after application of Example 2 according to an experimental example of the present invention.
3A is a photograph showing a change in skin thickness with time after application of Example 2 according to an experimental example of the present invention.
3B is a graph showing a change in skin thickness with time after application of Example 2 according to an experimental example of the present invention.
Figure 3c is a graph showing the increase/decrease rate of skin thickness according to time after application of Example 2 according to an experimental example of the present invention.
4A is a photograph showing changes in skin wrinkle improvement after application of Example 2 according to an experimental example of the present invention.
Figure 4b is a photograph showing the change of the indentation (Indentation) value showing the improvement of skin wrinkles after application of Example 2 according to an experimental example of the present invention.
Figure 4c is a graph showing the reduction rate of the indentation (Indentation) value showing improvement in skin wrinkles after application of Example 2 according to an experimental example of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Preparation Example 1. Manufacturing process of bio-fermented product

1-1. Preparation of Mushroom Fermented Products

In order to prepare the mushroom fermented product, the multi-faceted shield mushroom ( Albarellus confluens , Finnish supply and demand, powder) was used among the genus of Mushrooms. Distilled water was added in an amount corresponding to 20 times the weight of the multifaceted mushroom powder, and mixed and stirred to prepare a distilled water powder solution. The distilled water powder solution was exported with hot water at 121 °C for 15 minutes, and the distilled water powder extract was pre-fermented.

A 70% ethanol powder solution was prepared by adding 70% ethanol by weight of 10 times the weight of the polycystic mushroom ethanol extract. The 70% ethanol powder solution was stirred at 45° C. for 3 hours, centrifuged at 6000 rpm for 20 minutes, filtered at 0.45 μm, concentrated and extracted with 70% ethanol powder.

To 100 g of 5 mg/mL of the distilled water powder extract extracted with hot water, 1 g of 70% ethanol powder 10 mg/mL of saccharomyces mushroom was added, and Saccharomyces cerevisiae was cultured for 24 hours in a nutrient medium (YM broth, BD). Shia (Saccharomyces cerevisiae, KCTC7296) was inoculated with 5 g of a culture medium and fermented at 30 ° C. for 72 hours to prepare a fermentation extract.

The fermentation extract was sterilized at 121° C. for 15 minutes, centrifuged at 6000 rpm for 20 minutes, and filtered at 0.2 μm to obtain a supernatant, a fermented mushroom.

1-2. Confirmation of Gripoline Content of Mushroom Fermented Product

Grifolin was confirmed by high-performance liquid chromatography (HPLC) analysis of the mushroom ferment (Albatrellus confluens ferment extract). Specifically, it was detected at UV 232 nm with a Waters 2695 & 996 instrument. The column used was performed at 25 °C with an Agilent Eclipse (Agilent Eclipse XDB-C18, 5 μm, 4.6 x 150 mm). The mobile phase was 75% acetonitrile, and 0.01% phosphoric acid was added to 25% distilled water at a flow rate of 1.0 ml/min and an inflow rate of 20 ul. As a result of the experiment, it was confirmed that the mushroom fermented product contained grifolin.

Preparation Example 2. Preparation of Examples

Examples 1 to 11 were prepared according to each component and content shown in Table 1 below.

number composition ingredient Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 One solvent Purified water 84.9449 84.9449 84.9449 84.9449 84.9449 83.9449 86.4449 85.4449 83.9449 80.9449 76.4449 2 moisturizer propanediol 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3.0000 3 moisturizer methyl propanediol 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 4 moisturizer glycerin 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 5 moisturizer dipropylene glycol 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 6 sugar alcohols
Ryu erythritol 2.0000 - - - - 2.0000 0.5000 0.5000 1.0000 2.0000 3.5000 7 sugar alcohols xylitol - 2.0000 - - - 0.5000 - 0.5000 1.0000 2.0000 3.5000 8 sugar alcohols Trehalose - - 2.0000 - - 0.5000 - 0.5000 1.0000 2.0000 3.5000 9 sugar alcohols sorbitol - - - 2.0000 - - - - - - - 10 sugar alcohols maltitol - - - - 2.0000 - - - - - - 11 fermented product Mushroom Fermented 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 12 viscosity increasing agent Carbomer 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 0.1500 13 pH adjuster tromethamine 0.150 0.150 0.150 0.150 0.150 0.150 0.150 0.150 0.150 0.150 0.150 14 skin soothing agent allantoin 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 0.1000 15 emulsifier PEG-40 Hydrogenated Castor Oil 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 16 emulsifier Polyglyceryl-10 Laurate 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 17 emulsifier Polyglyceryl-10 Myristate 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 0.0200 18 sequestering agent Disodium EDIT 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 0.0100 19 conditioning agent Sodium Hyaluronate 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 0.0500 20 conditioning agent Biosaccharide Gum-1 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 21 antioxidant tocopherol 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 22 preservative 1,2-Hexanediol 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 2.5000 Total (wt%) 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000 100.0000

Weigh the raw materials according to the composition ratio of Table 1, and propanediol, methylpropanediol, glycerin, dipropylene glycol, sugar alcohols (erythol, xylitol, trehalose, sorbitol or maltitol), allantoin, disodium EdTA and sodium hyaluronate were put in cold purified water, and then dispersed and dissolved with a homogenizer at 2000 rpm for 10 minutes to prepare a mixed solution 1.

Thereafter, the carbomer was dispersed, mixed with the mixed solution 1, and then dispersed and dissolved with a homogenizer at 2000 rpm for 10 minutes to prepare a mixed solution 2.

Tromethamine was added to the prepared mixture 2 and dispersed for 10 minutes at 3000 rpm with a homogenizer, and again oil-soluble components tocopherol, PEG-40 hydrogenated castor oil, polyglyceryl-10 laurate and polyglyceryl-10 Mixture 3 was prepared by dispersing the myristate for 5 minutes at 2000 rpm with a homogenizer after mixing and dispersing.

Finally, the prepared mixed solution 3 was cooled to 25° C., and then fermented mushrooms, biosaccharide gum-1 and an appropriate amount of a preservative were added. Then, by cooling and defoaming to 25 ℃ Examples 1 to 11 were prepared.

Preparation Example 3. Preparation of Comparative Example

Comparative Examples 1 to 4 were prepared according to each component and content described in Table 2 below.

number composition ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 One solvent Purified water 86.9949 86.9449 84.9949 81.9949 2 moisturizer propanediol 3.0000 3.0000 3.0000 3.0000 3 moisturizer methylpropanediol 2.0000 2.0000 2.0000 2.0000 4 moisturizer glycerin 2.0000 2.0000 2.0000 2.0000 5 moisturizer dipropylene glycol 2.0000 2.0000 2.0000 2.0000 6 sugar alcohols erythritol - - - - 7 sugar alcohols xylitol - - - - 8 sugar alcohols Trehalose - - - - 9 sugar alcohols sorbitol - - - - 10 sugar alcohols maltitol - - - - 11 fermented product Mushroom Fermented - 0.0500 2.0000 5.0000 12 viscosity increasing agent Carbomer 0.1500 0.1500 0.1500 0.1500 13 pH adjuster tromethamine 0.150 0.150 0.150 0.150 14 skin soothing agent allantoin 0.1000 0.1000 0.1000 0.1000 15 emulsifier PEG-40 Hydrogenated Castor Oil 0.0500 0.0500 0.0500 0.0500 16 emulsifier Polyglyceryl-10 Laurate 0.0200 0.0200 0.0200 0.0200 17 emulsifier Polyglyceryl-10 Myristate 0.0200 0.0200 0.0200 0.0200 18 sequestering agent Disodium EDIT 0.0100 0.0100 0.100 0.0100 19 conditioning agent Sodium Hyaluronate 0.0500 0.0500 0.0500 0.0500 20 conditioning agent Biosaccharide Gum-1 1.0000 1.0000 1.0000 1.0000 21 antioxidant tocopherol 0.0001 0.0001 0.0001 0.0001 22 preservative 1,2-Hexanediol 2.5000 2.5000 2.5000 2.5000 Total (% by weight) 100.0000 100.0000 100.0000 100.0000

Weigh the raw materials according to the composition ratio in Table 2, and add propanediol, methylpropanediol, glycerin, dipropylene glycol, allantoin, disodium EDTA and sodium hyaluronate to cold purified water, and then homoge Mixture 1 was prepared by dispersing and dissolving for 10 minutes with a Niger 2000 rpm.

Thereafter, the carbomer was dispersed, mixed with the mixed solution 1, and then dispersed and dissolved with a homogenizer at 2000 rpm for 10 minutes to prepare a mixed solution 2.

Tromethamine was added to the prepared mixture 2 and dispersed for 10 minutes at 3000 rpm with a homogenizer, and again oil-soluble components tocopherol, PEG-40 hydrogenated castor oil, polyglyceryl-10 laurate and polyglyceryl-10 Mixture 3 was prepared by dispersing the myristate for 5 minutes at 2000 rpm with a homogenizer after mixing and dispersing.

Finally, the prepared mixed solution 3 was cooled to 25° C., and then fermented mushrooms, biosaccharide gum-1 and an appropriate amount of a preservative were added. Then, by cooling and defoaming to 25 °C, Comparative Examples 1 to 4 were prepared.

Experimental Example 1. Cooling Durability Test

After placing the mask sheet fabric (2 X 2 cm) on the inner side of the lower arm, 100 ul of the composition of Example 1 and Comparative Example 2 was applied on the fabric, and then the test site was inspected with an IRIS-X (Gold) infrared camera. Changes in time before and after application were observed, and the results are shown in Tables 3 and 4, and FIGS. 1A to 1C.

(Unit: ℃) before application immediately after application after 1 minute after 3 minutes 5 minutes later after 10 minutes after 15 minutes uncoated 26.40 26.28 26.16 25.94 25.94 25.85 25.8 Example 1 26.35 24.38 23.92 23.54 23.45 23.35 23.75 Comparative Example 2 26.24 24.47 24.22 24.01 23.94 24.03 24.34

(unit: %) immediately after application after 1 minute after 3 minutes 5 minutes later after 10 minutes after 15 minutes uncoated -0.40 -0.9 -1.1 -1.7 -2.1 -2.3 Example 1 -7.5 -9.2 -10.7 -11 -11.4 -10.5 Comparative Example 2 -6.8 -7.7 -8.5 -8.8 -8.4 -7.3

As shown in Table 3, Table 4, and FIGS. 1A to 1C, as a result of measuring the temperature 15 minutes after application, Example 1 was 23.67 °C, Comparative Example 2 was 24.34 °C. And Example 1 showed a temperature reduction rate of -10.5% and Comparative Example 2 -7.3%. Example 1 includes a mushroom fermented product and erythritol, a sugar alcohol as a cooling agent, but Comparative Example 2 has a difference in that it includes only a mushroom fermented product, and Example 1 has an excellent effect in reducing skin temperature than Comparative Example 2 indicated.

Through this, it was confirmed that a remarkable cooling effect was exhibited when the mushroom fermented product and sugar alcohols were included in a ratio of 1:40 to 60 rather than each containing the mushroom fermented product or sugar alcohols.

Experimental Example 2. Sensory test

Five subjects with an average age of 28.4 years were selected and attached to the lower arm area using a square patch-type sheet (3 * 3 cm), and then Examples 1 to 11 and Comparative Examples 2 to 4 were applied. After application, a sensory test was performed with respect to the feeling of coolness, stickiness, and absorption felt when applied, and the results are shown in Table 5 below.

Regarding the durability of feeling of cooling felt when applied, Comparative Example 1 without a cooling agent was used as a control, and 3 points were set as a reference point, and a score was given out of 5 points to give an average. Other stickiness and absorbency were calculated through the following evaluation criteria, and then average preference was calculated.

<Evaluation criteria>

◎: very high; ○: high; △: normal; X: Not good.

division Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Example 10 Example 11 longevity of cold 3.00 3.10 3.04 3.12 4.44 4.27 4.21 3.56 3.42 4.25 3.49 3.84 4.38 4.86 4.92 stickiness △ △ △ △ △ △ △ △ △ ○ △ △ ○ ◎ ◎ absorptivity ○ ○ ○ ○ △ △ △ △ △ △ △ △ △ X X

As shown in Table 5, the examples including the mushroom fermented product and sugar alcohols received high scores, and the feeling of cold persistence increased in proportion to the total content of sugar alcohols.

In particular, Examples 1 to 3 containing sugar alcohols in the range of 2.0000 to 10.5000 wt% received good evaluations in terms of stickiness and absorption power as well as cooling lasting power. On the other hand, Example 9 contains 3.0000 weight %, and Example 10 contains 6.0000 weight % of sugar alcohols. Both Examples 9 and 10 had excellent cooling durability, but were not suitable for use as a cosmetic composition due to high stickiness and slow absorption.

In addition, Example 11, which contained the most sugar alcohols at 10.5000 wt %, showed the highest value in terms of durability, but had very high stickiness and poor absorption. On the contrary, Example 7 containing 0.5000 weight % of sugar alcohols and Example 8 containing 1.5000 weight % had normal stickiness and absorption power, but showed lower cooling persistence than Examples 1 to 3. In addition, among the sugar alcohols, Example 4 containing sorbitol and Example 5 containing maltitol showed lower cooling persistence than Examples 1 to 3.

Therefore, when it contains more or less specific sugar alcohols, it may affect the cooling durability, stickiness, and absorption power, and the most suitable is erythritol, xylitol and trehalose in 2.0000 to 10.5000 weight % in mushroom fermented product. It was confirmed that the most optimal effect was exhibited when

Experimental Example 3. Evaluation of increase or decrease in pore area

Five subjects with an average age of 29.4 years were selected to evaluate the increase or decrease in pore area. Before and after the increase or decrease of the pore area, the pore area of the test site was analyzed and comparatively evaluated. Affected area means the total area of pores recognized by the device among the areas designated as analysis areas among the test areas. Specifically, after washing the face, wait for 10 minutes in a constant temperature and humidity room (22±2 ℃, 40 to 60 % RH), and then take a photo of the pores of the test site (before) with antenna 3D (Antera 3D, miravex, UK) and iris. -XP(GOLD) (IRIS-X P(GOLD), MEDICORE, Korea) was taken. After applying Example 1 to the face, pore photos (after) were again taken with the same device, and the results are shown in Table 6 and FIGS. 2A to 2C.

Before using the product after using the product Affected Area(mm ² ) 34.39 22.45 Pore area increase/decrease rate (%) 34.7

As shown in Table 6 and FIGS. 2a to 2c, before using Example 1, the area of pores was 34.39 mm ² , but after 10 minutes of using the product, the area of pores was 22.45 mm ² , which decreased by about - 34.7%. Through this, it was confirmed that, as in Example 1, when the mushroom fermented product and erythol, a sugar alcohol, were included in a ratio of 1:40, there was a significant cooling effect and an effect of pore reduction.

Experimental Example 4. Evaluation of skin thickness improvement

Five subjects with an average age of 36.4 years were selected to evaluate the improvement in skin thickness. Skin thickness photos were taken for each week, and the analysis value of thickness (um) and the value of week 0 were compared and evaluated, and it was judged that the skin thickness improved as the thickness increased. Specifically, after washing the face with ivory soap, wait in a constant temperature and humidity room (22±2 ℃, 40 to 60 % RH) for 10 minutes, and then measure the skin thickness (0 weeks) with antenna 3D (Antera 3D, miravex, UK) and It was taken with DUB Skinscanner (DUB Skinscanner, EOTECH, France). After using Example 2 for 2 weeks and 4 weeks, the thickness of the skin was photographed for each week in the same manner as at week 0, and the results are shown in Tables 7, 8, and 3A to 3C.

0 weeks 2 weeks 4 weeks skin thickness (um) 619 737 837

2 weeks 4 weeks Skin thickness (%, versus week 0) 19.1 35.2

As shown in Table 7, Table 8, and FIGS. 3A to 3C, the skin thickness after 2 weeks of use of Example 2 was 737 um, and after 4 weeks, the skin thickness was 836 um, which was 19.1% and 35.2%, respectively. As it appears, it was confirmed that as the period of use of Example 2 increased, the skin density increased and the skin thickness was improved.

Experimental Example 5. Skin wrinkle improvement evaluation

The improvement of skin wrinkles was evaluated by selecting 5 subjects with an average age of 36.4 years. Skin thickness photos were taken for each week, and the analysis value of thickness (um) and the value of week 0 were compared and evaluated. After designating the same area of the measurement site, the extent of wrinkle improvement was quantified by analyzing the indentation, which is a comprehensive value by measuring the area and depth of the area lowered from the skin surface. It was judged that the wrinkles were improved as the indentation value decreased, and wrinkles having a thickness of 0.5 mm or less were selected and analyzed.

Specifically, after washing the face with ivory soap and waiting in a constant temperature and humidity room (22±2 ℃, 40 to 60 % RH), skin wrinkles (0 weeks) were treated with antenna 3D (Antera 3D, miravex, UK) and DUB. After photographing with a skin scanner (DUB Skinscanner, EOTECH, France), the indentation value was measured. After using Example 2 before bedtime for 2 weeks and 4 weeks, Example 2 was used once a day, skin wrinkles (2 weeks and 4 weeks) were photographed for each week in the same manner as at week 0, and the indentation value was measured Thus, the results are shown in Table 10, Table 11, and FIGS. 4A to 4C. The indentation value means a value comprehensively expressed by measuring the area and depth of the lowered portion of the skin surface, and it is determined that wrinkles are improved as the indentation value decreases.

division 0 weeks 2 weeks 4 weeks Indentation (A.U) 5.186 4.834 4.583

division 2 weeks 4 weeks Indentation (%, versus week 0) -6.8 -11.6

As shown in Table 9, Table 10, and FIGS. 4A to 4C , the indentation value after 2 weeks of use of Example 2 was 4.834 AU , and the indentation value after 4 weeks of use was 4.583 AU, respectively -6.8% and -11.6% appeared to decrease. Therefore, it means that the longer the period of use of Example 2, the more effective the wrinkle improvement is.

Claims (12)

A cooling cosmetic composition comprising a mushroom fermented product and sugar alcohols. The cosmetic composition according to claim 1, wherein the weight ratio of the mushroom fermented product and sugar alcohol is 1:40 to 60. The method according to claim 1, wherein the mushroom is selected from: Albatrellus confluens (Alb. & Schwein.) Kotl. & Pouz.), Albatrellus ovinus (Schaeff.) Kotl. & Pouz. Albatrellus dispansus (Lloyd) Canf. & Gilb.), green shield mushroom ( Albatrellus caeruleoporus (Peck) Pouz.), gray-blue shield mushroom ( Albatrellus yasudae (Lloyd) Pouzar), long-legged shield mushroom ( Albatrellus pes-caprae ), purple Face mushroom ( Albatrellus cristatus ) and rough hairy shield mushroom ( Albatrellus ellisii ) The cosmetic composition comprising at least one selected from the group consisting of. The cosmetic composition of claim 1, wherein the mushroom fermented product is contained in an amount of 0.0500 to 2.0000 wt% based on the total weight of the cosmetic composition. The cosmetic composition according to claim 1, wherein the sugar alcohol is at least one selected from the group consisting of erythritol, xylitol, trehalose, sorbitol and maltitol. The cosmetic composition of claim 1, wherein the sugar alcohol is included in an amount of 2.0000 to 10.5000 wt% based on the total weight of the cosmetic composition. The cosmetic composition of claim 1, wherein the cooling cosmetic composition further comprises at least one selected from the group consisting of a moisturizing agent, a viscosity increasing agent, a skin soothing agent, an emulsifier, a conditioning agent, a sequestering agent, and an antioxidant. . A method for producing a cooling feeling cosmetic composition comprising the steps of:
Mushroom fermented product manufacturing step of preparing the mushroom fermented product; and
A sugar alcohol mixing step of mixing the mushroom fermented product and sugar alcohols. The method according to claim 8, wherein the step of preparing the mushroom fermented product comprises the following steps:
A powder solution preparation step of mixing and stirring by adding a solvent to the mushroom powder;
An extraction step of extracting the powder liquid into a powder extract or powder; and
A step of preparing a powdered extract or a fermentation extract in which the powder is fermented with an enzyme or yeast. The cosmetic composition according to claim 8, wherein the weight ratio of the mushroom fermented product and sugar alcohol is 1:40 to 60. The method according to claim 8, wherein the mushroom is selected from: Albatrellus confluens (Alb. & Schwein.) Kotl. & Pouz.), Albatrellus ovinus (Schaeff.) Kotl. & Pouz. Albatrellus dispansus (Lloyd) Canf. & Gilb.), green shield mushroom ( Albatrellus caeruleoporus (Peck) Pouz.), gray-blue shield mushroom ( Albatrellus yasudae (Lloyd) Pouzar), long-legged shield mushroom ( Albatrellus pes-caprae ), purple Face mushroom ( Albatrellus cristatus ) and rough hairy shield mushroom ( Albatrellus ellisii ) The cosmetic composition comprising at least one selected from the group consisting of. The cosmetic composition according to claim 8, wherein the sugar alcohol is at least one selected from the group consisting of erythritol, xylitol, trehalose, sorbitol and maltitol.

Images