KR20220037662A - Composition for Improving intestine function comprising 'Noeulchal'sorghum - Google Patents

Abstract

The present invention relates to a prebiotics composition including Noeulchal sorghum as an active component, a health functional food for intestine function improvement including the composition, and an intestine function improvement method using the same. The prebiotics composition can be usefully used to promote intestine function improvement by accelerating growth of beneficial bacteria in an intestine and suppressing growth of harmful bacteria in the intestine, thereby being used in reduction, prevention and the like of a metabolic disease, an irritable bowel syndrome, and proliferative enteritis.

Description

Composition for Improving intestine function comprising 'Noeulchal'sorghum}

The present invention provides a prebiotic composition comprising noulchal sorghum as an active ingredient; Health functional food for improving intestinal function comprising the composition; And it relates to a method for improving intestinal function using the same.

The symbiotic microbiome in the human gut affects many aspects of human health. Intestinal microorganisms ferment polysaccharides (resistant starch, oligosaccharides, inulin, etc.) that cannot be digested in the stomach and small intestine by microorganisms in the large intestine to produce short chain fatty acids (SCFA), which provide nutrients and energy to the host. and competes to prevent colonization of opportunistic bacteria in the intestine, and is involved in the formation and maintenance of the immune system.

However, it has been reported that the imbalance of the intestinal flora is related to host infectious diseases, gastrointestinal diseases, diabetes and obesity. Diet can greatly influence the composition of the intestinal microbial community in the short and long term, which means that changes in the intestinal microflora through the diet can improve the health of the host. As a result, in the food market, many studies have been conducted targeting the intestinal microbes.

Probiotics are living microorganisms that can work beneficially by maintaining the balance of the human intestinal flora. The probiotics inhibit the growth of harmful bacteria through a decrease in intestinal pH by production of short chain fatty acids (SCFA) and competition for intestinal nutrients and barrier attachment sites.

It is also known to have beneficial functions in the human body, such as immune activation and alleviation of lactose intolerance. Representative microorganisms used as probiotics include bifidobacteria and bacteria belonging to lactic acid bacteria (LAB). Lactic acid bacteria are bacteria that produce lactic acid by fermenting carbohydrates, and include Lactobacillus sp. strains, Streptococcus sp. strains, Leukonostok sp. strains, and the like. On the other hand, bifidobacteria are bacteria that use various oligosaccharides as energy sources, and in the process of converting hexoses into lactic acid or acetic acid, an enzyme called fructose-6-phosphate phosphoketolase acts, and fermentation It has a unique characteristic that it does not generate gas in the process.

The main ingredient used to more effectively proliferate and maintain the probiotics in the intestine is called prebiotics. The prebiotics are not digested by humans, but may be defined as substances that are selectively fermented by intestinal microbes to control their growth and metabolic changes, thereby helping the health of the host. As a representative example of the prebiotics, fructo-oligosaccharides (FOS) are known. The fructooligosaccharide is an oligosaccharide in which fructose molecules are combined with sugar in β-1, 2, and since fermentation is possible by Lactobacillus and Bifidobacteria, ingestion of FOS increases Bifidobacterium and Lactobacillus species. It is known that it can be done. However, the FOS can have a beneficial effect on humans by increasing the intestinal probiotic strain, but there is a known problem that the adverse effect may appear by harmful bacteria and non-probiotics that can metabolize the intestinal FOS. , there is a demand for the development of a prebiotic composition that can more effectively proliferate and maintain probiotics, and research for developing this is being actively conducted. For example, Korean Patent No. 10-1973514 discloses prebiotics containing grape seed powder.

Under this background, the present inventors have made diligent efforts to develop a more effective prebiotic composition, confirmed that Noeulchal sorghum can be used as an active ingredient of the prebiotic composition, and completed the present invention.

One object of the present invention is to provide a prebiotic composition comprising Noeulchal sorghum as an active ingredient.

Another object of the present invention is to provide a health functional food for improving intestinal function comprising the composition.

Another object of the present invention is to provide a method for improving intestinal function of an individual comprising administering the composition to an individual other than humans.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention for achieving the above object provides a prebiotic composition comprising Noeulchal sorghum as an active ingredient.

As used herein, the term "Noeulchal sorghum" is one of sorghum varieties, and specifically, the soybean length is 93 cm short, the ear shape is medium, the side shape is ovate, the seed color is light brown, and the endosperm characteristics are sticky, It is known that the stem diameter is 22mm, thin, and the ear length is 9cm long, and the number of days of seeding is 66 days.

The sorghum is an annual grass of the monocotyledonous plant family, also called goryang (高梁), sorghum, furrow, and losok (蘆粟), and is used in various countries as bread, porridge, alcoholic and non-alcoholic beverages, etc. is known

Sorghum is known as a functional multi-grain because it is rich in health functional ingredients such as polyphenols and flavonoids and contains a large amount of dietary fiber.

The sunset sorghum may be used in powder form, but is not limited thereto.

In a specific embodiment of the present invention, when sorghum bread is prepared using Noeulchal sorghum rich in the above functional ingredients and taken, the effect of inhibiting the growth of harmful microorganisms and promoting the growth of useful microorganisms in the intestines and intestinal function resulting therefrom The improvement effect was confirmed.

As used herein, the term "prebiotics" or a prebiotic composition refers to an indigestible component that is mainly used by useful microorganisms in the large intestine to promote the growth or activity of microorganisms, thereby exhibiting a good effect on health. .

In the present invention, the prebiotic composition contains noelchal sorghum as an active ingredient, thereby promoting the growth of beneficial intestinal bacteria and inhibiting the growth of harmful intestinal bacteria.

As used herein, the term "intestinal beneficial bacteria" refers to beneficial bacteria that previously inhabit the host's intestine and strains that have a beneficial effect on the intestinal environment when ingested and reach the intestine. These are bacteria that survive in gastric and bile acids, reach the small intestine, multiply and settle in the intestine, exhibit useful effects in the intestine, and satisfy the non-toxic and non-pathogenic conditions.

In the present invention, the beneficial bacteria in the intestine is not particularly limited thereto, but as an example, it may be a Eubacterium sp. strain.

As used herein, the term "intestinal harmful bacteria" refers to harmful bacteria existing in the intestine of the host and strains that are harmful to the intestinal environment when ingested and reach the intestine.

In the present invention, the intestinal harmful bacteria are not particularly limited thereto, but as an example, Alistipes sp., Desulfitobacterium sp., and Lawsonia sp. It may be any one or more selected from.

In a specific embodiment of the present invention, the effect of improving intestinal function through reduction of the F/B ratio was confirmed through ingestion of Noeulchal millet bread, and through this, the possibility of application to improvement of obesity, one of metabolic diseases, was also confirmed.

In another embodiment, the reduction of the irritable growth syndrome-related Alistypes sp. strain corresponding to the harmful bacteria, the reduction of the sulfur compound-producing Desulfitobacterium sp. strain and the reduction of the Lausonia sp. strain causing proliferative enteritis are reduced, while the beneficial bacteria Yubak It was confirmed that the terium sp. strain was increased, and thus it was confirmed that it had the effect of improving usability and intestinal function as a prebiotic composition. In addition, it was confirmed that it can be used for the improvement of metabolic diseases, irritable bowel syndrome, and proliferative enteritis.

Another aspect of the present invention for achieving the above object provides a food or health functional food for improving intestinal function comprising the prebiotic composition.

The prebiotic composition of the present invention is safe by using Noulchal sorghum, which is one of the grains, and is effective in improving intestinal function by increasing beneficial intestinal bacteria and decreasing harmful bacteria when ingested. It can be useful.

In this case, the definition of the prebiotic composition and Noeulchal sorghum is the same as described above.

As used herein, the term “improvement” refers to any action of reducing a parameter related to a condition to be treated by ingestion of the food composition, for example, the severity of symptoms.

The "improvement of intestinal function" of the present invention refers to the irritable bowel syndrome-related Alistypes spp., the sulfur compound-producing Desulfitobacterium spp. and the proliferative enteritis-inducing Lausonia sp. strain, which are harmful intestinal bacteria, while inhibiting the growth of beneficial bacteria. It may mean promoting intestinal health through improvement of the intestinal flora through the promotion of the proliferation of Phosphorus Eubacterium spp., and improvement of metabolic diseases, irritable bowel syndrome, and proliferative enteritis through inhibition of proliferation of harmful bacteria and increase of beneficial bacteria , prevention, etc., but is not limited thereto.

As used herein, the term "food" refers to meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages , vitamin complexes, health functional foods, and health foods, and includes all foods in the ordinary sense.

As used herein, the term "health functional food" is the same term as food for special health use (FoSHU), and in addition to nutrient supply, medicine processed to efficiently exhibit bioregulatory functions, It means food with high medical effect.

Here, 'function (sex)' refers to obtaining useful effects for health purposes, such as regulating nutrients or physiological actions with respect to the structure and function of the human body. The health food means food having an active health maintenance or promotion effect compared to general food, and health supplement food means food for the purpose of health supplementation. In some cases, the terms health functional food, health food, and health supplement may be used interchangeably.

Specifically, the health functional food is added to food materials such as bread, dairy products, beverages, teas, spices, gum, confectionery, etc., or encapsulated, powdered, suspension, etc. It is a food manufactured with , meaning that it brings specific health effects when ingested, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material.

The food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art.

In addition, the food composition may be prepared without limitation in various types of formulations as long as the formulations are recognized as food.

In addition, the food composition may further include a physiologically acceptable carrier, the type of carrier is not particularly limited and any carrier commonly used in the art may be used.

In addition, the food composition may include additional ingredients that are commonly used in food compositions to improve odor, taste, and vision. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, pantothenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), chromium (Cr); and amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the food composition includes a preservative (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), a disinfectant (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), an antioxidant (butylhydroxyanisole (BHA), butyl hydro Loxytoluene (BHT), etc.), coloring agents (tar pigments, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclamate, saccharin, etc.) , sodium, etc.), flavorings (vanillin, lactones, etc.), swelling agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (foaming agents), film agents, gum base agents, foam inhibitors, solvents, improving agents, etc. It may contain food additives. The additive may be selected according to the type of food and used in an appropriate amount.

Another embodiment of the present invention provides a method for improving intestinal function in an individual, comprising administering the prebiotic composition to the individual.

As described above, since the prebiotic composition containing noulchal sorghum as an active ingredient can exhibit the effect of promoting the growth of beneficial intestinal bacteria and inhibiting the growth of harmful intestinal bacteria, when the prebiotic composition is administered to an individual , to enhance the intestinal function of the subject.

In the present invention, the term "individual" includes, without limitation, mammals including mice, livestock, humans, etc., which are intended to promote intestinal health.

In the method of promoting intestinal health of the present invention, the administration route of the prebiotic composition may be administered through any general route. The prebiotic composition of the present invention is not particularly limited thereto, but may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, oral administration, intranasal administration, intrapulmonary administration, or rectal administration as desired. And, preferably, it can be administered orally. In addition, the composition may be administered by any device capable of transporting the active agent to a target cell.

The prebiotic composition comprising Noeulchal sorghum as an active ingredient of the present invention can be usefully utilized to promote the growth of beneficial intestinal bacteria and to improve intestinal function by inhibiting the growth of harmful intestinal bacteria.

In addition, it will be possible to use for the improvement and prevention of metabolic diseases, irritable bowel syndrome, and proliferative enteritis by inhibiting the growth of harmful bacteria in the intestine and increasing beneficial bacteria.

1 is for the intestinal Firmicutes / Bacteroidetes (F / B) ratio according to the intake of regular bread or Noeulchal millet bread.
Figure 2 is about changes in the intestinal microbial community according to the intake of ordinary bread or Noeulchal millet bread.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1: Preparation of Noeulchal Millet Bread

For the sorghum sample, Noeulchal sorghum cultivar cultivated in 2019 by the Southern Crop Department of the National Academy of Food Science, Rural Development Administration was used.

Using the sorghum powder pulverized by the Noeulchal sorghum into 100 to 200 mesh, a wheat flour to sorghum powder content ratio was 50:50 to prepare sorghum bread. The morning bread used as a control was made with 100% wheat flour.

The ratio of wheat flour or water powder used for morning bread production was adjusted to 50% (300g), and the other ingredients were sugar 20g, yeast 6g, salt 3g, milk 220ml, butter 30g, and water 15ml. The raw materials were mixed and the first fermentation was carried out for 50 minutes, and the dough was divided into 16 pieces to make a round shape, followed by the second fermentation for 50 minutes, and baked at 190°C to 200°C for 12 minutes to complete the morning bread.

Example 2. Confirmation of long-term improvement effect of Noeulchal millet bread

2-1. Measurement of microbial community changes in mouse feces using millet bread

For the experimental mice used in this study, 7-week-old BALB/c male mice were used, and after a one-week adaptation period in a constant temperature and humidity condition, they were used for the experiment. For the sample administered to the mouse, an experiment was carried out using the dried and pulverized powder of the morning bread prepared using the sorghum and wheat flour. 10 mice in each group were randomly assigned, and the mice were orally administered twice a day at 2,500 mg/kg for 14 days. The body weight of the mice was measured every 7 days.

Feces were collected before euthanasia of mice by inhalation of carbon dioxide, and total microbial DNA in feces was isolated using the QIAamp ^® DNA Mini Kit (Qiagen) according to the manufacturer's method. The isolated total microbial DNA concentration was quantified with nanodrop (Ependorp Biospetrometer D30) and metagenome analysis was analyzed in Macrogen.

Statistical significance was verified using IBM SPSS 25 software, and statistical analysis was performed by student t-test or Mann-Whitney test according to normality. The P-value was accepted only when it was less than 0.05.

2-2. Analysis of changes in the microbial community in feces

Microorganisms in feces collected after oral administration of each sample to mice were checked for OTUs at the phylum level (Table 1). Fimicutes phylum OTUs were statistically significantly decreased in millet bread compared to normal bread as a control, and other microorganisms were not significantly changed.

Changes in microbial OTUs at the phylum level Phylum Plain bread (contrast) millet bread Bacteroidetes 11294.25 ± 2068.39 12659.50 ± 4534.40 Firmicutes 10355.88 ± 1687.99 8580.63 ± 1357.72 ^*

^* P < 0.05 vs. plain bread

Firmicutes phylum and Bacteroidetes phylum account for most of the bacteria in the large intestine, and it is known that the sclerites play an important role in increasing fat absorption.

Also, it has been reported that the change in its ratio is related to obesity, and the Firmicutes/Bacteroidetes (F/B) ratio has a significant correlation with BMI, and as the F/B ratio increases, the body mass index (BMI) also increases. It is known to increase (Koliada et al., 2017). In addition, it has been reported that obese people have a statistically significantly higher F/B ratio than the general public (Ismail et al., 2011).

Accordingly, when the Noeulchal millet bread was consumed, as can be seen in Table 1 and FIG. 1, in this experiment, the group administered with millet bread significantly decreased the F/B ratio compared to the group administered with regular bread as a control. Functional improvement was confirmed, and the possibility of using it to improve metabolic diseases such as obesity was also confirmed.

2-3. Changes in the genus level community of intestinal microbes of Noeulchal millet bread

Microorganisms in feces collected after oral administration of each sample to mice were checked for OTUs at the genus level. Among all genus, there were 66 microorganisms with a ratio of more than 0.05% to the total microorganisms, and among them, Alistipes sp., Eubacterium sp., and Desulfitobacterium sp.), there was a significant difference in Lawsonia sp.

Among the microorganisms, the Eubacterium sp. strain was significantly increased in the group administered with millet bread than the group administered with normal bread, and microorganisms of the genera Alistypes, Desulfitobacterium, and Lausonia were treated with millet bread. The group was significantly reduced compared to normal bread (Table 2 and Figure 2).

Changes in microbial OTUs at the Genus level Genus Plain bread (contrast) millet bread Aliipes 482 ± 198 173 ± 77 Eubacterium 11 ± 9 128 ± 95 Desulfitobacterium 131 ± 53 78 ± 24 Lawsonia 47 ± 17 13 ± 3

^*** P < 0.001 vs. Plain bread, ^** P < 0.01 vs. Plain bread, ^* P < 0.05 vs. plain bread

Among the microorganisms in Table 2, Alistypes sp. strain is a pathogenic bacteria and has been reported to be related to the frequency of abdominal pain of irritable bowel syndrome (Saulnier et al 2011), and it has been reported that there is a tendency to increase in mice subjected to stress (Bangsggard et al.) al 2012).

In addition, a correlation between the number of glycated hemoglobin and the genus of Alistypes in some type 2 diabetes patients by herbal medicine administration was reported (Xu et al, 2014).

As a result, it was confirmed that Noeulchal millet bread could be used to improve intestinal function by inducing a reduction in the strain of Alistypes genus, and to be usefully used for the improvement of irritable bowel syndrome and the improvement of metabolic diseases.

In addition, Eubacterium spp. strain is an intestinal microorganism that contributes to buteric acid production and has been reported to decrease in colorectal cancer patients (Zhang et al 2019). It was confirmed that it can be used effectively.

In addition, the genus Desulfitobacterium is a bacterium involved in sulfur metabolism (Lie et al. 1999), and by reducing the harmful bacteria in the group taking millet bread, it was possible to infer the effect of improving intestinal function through reduction of harmful sulfide production in the intestine. .

In addition, the Lawsonia intracellularis strain among the genus Lawsonia is an obligate intracellular enteropathogen that causes proliferative enteritis in several animals (Karuppannan et al. 2018).

It was confirmed that the Noeulchal millet bread significantly reduced the strain of Rausonia spp., a harmful bacterium that causes proliferative enteritis.

As can be seen in Table 2 and FIG. 2, Noulchal millet bread reduces the irritable growth syndrome-related Alistypes sp. strain corresponding to harmful bacteria, reduces the sulfur compound-producing Desulfitobacterium sp. strain, and induces proliferative enteritis lausonia. While the spp. strain was decreased, it was confirmed that the beneficial bacteria, Eubacterium sp. strain, increased, thereby confirming that the prebiotic composition had an effect and improved intestinal function.

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalents.

Claims (7)

Prebiotics composition comprising Noeulchal sorghum as an active ingredient.
According to claim 1,
The prebiotic composition promotes the growth of beneficial bacteria in the intestine and inhibits the growth of harmful bacteria in the intestine, the composition.
3. The method of claim 2,
The beneficial bacteria in the intestine is a strain of Eubacterium sp., the composition.
3. The method of claim 2,
The intestinal harmful bacteria are Alistipes genus ( Alistipes sp.), Desulfitobacterium genus ( Desulfitobacterium sp.), and Lawsonia genus ( Lawsonia sp.), the composition of any one or more selected from.
According to claim 1,
The Noelchal sorghum is in the form of a powder, the composition.
A health functional food for improving intestinal function comprising the prebiotic composition according to claim 1.
A method for improving intestinal function of an individual comprising administering the prebiotic composition of any one of claims 1 to 6 to an individual other than a human.

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