KR20190000512A - Method for nonsoluble dietary fiber manufacturing puffed snack with improving proliferation of probiotics in intestine - Google Patents
Method for nonsoluble dietary fiber manufacturing puffed snack with improving proliferation of probiotics in intestine Download PDFInfo
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- KR20190000512A KR20190000512A KR1020170079606A KR20170079606A KR20190000512A KR 20190000512 A KR20190000512 A KR 20190000512A KR 1020170079606 A KR1020170079606 A KR 1020170079606A KR 20170079606 A KR20170079606 A KR 20170079606A KR 20190000512 A KR20190000512 A KR 20190000512A
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- grain
- expanded
- powder
- growth
- rice
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- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/0002—Processes of manufacture not relating to composition and compounding ingredients
- A23G3/0004—Processes specially adapted for manufacture or treatment of sweetmeats or confectionery
- A23G3/0019—Shaping of liquid, paste, powder; Manufacture of moulded articles, e.g. modelling, moulding, calendering
- A23G3/0042—Moulding or shaping of cellular or expanded articles
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/0002—Processes of manufacture not relating to composition and compounding ingredients
- A23G3/0004—Processes specially adapted for manufacture or treatment of sweetmeats or confectionery
- A23G3/0006—Manufacture or treatment of liquids, pastes, creams, granules, shred or powder
- A23G3/0017—Transformation of a liquid, paste, cream, lump, powder, granule or shred into powder, granule or shred; Manufacture or treatment of powder
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- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/161—Puffed cereals, e.g. popcorn or puffed rice
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/32—Foods, ingredients or supplements having a functional effect on health having an effect on the health of the digestive tract
- A23V2200/3202—Prebiotics, ingredients fermented in the gastrointestinal tract by beneficial microflora
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Abstract
Description
본 발명은 장내 프로바이오틱을 증진하는 팽화 곡물 과자 및 이의 제조방법을 제공하는 것이다. The present invention provides expanded wheat flour for promoting intestinal probiotics and a method for producing the same.
식이섬유(dietary fiber)는 대장 내 유익 미생물에 의해 이용되어 미생물의 생육이나 활성을 촉진함으로써 숙주 건강에 좋은 효과를 나타내게 하는 비소화성 식품성분인 프리바이오틱스(prebiotics) 물질로서, 혈중 인슐린, 글루코오스, 중성지방, 콜레스테롤 등의 농도 상승을 억제하고, 식사 섭취량을 감소시키며, 체중 증가를 억제하고, 체지방을 감소시키는 효과가 있을 뿐만 아니라, 변비 개선과 당뇨병 환자의 내당능을 개선하는 효과가 있는 것으로 알려져 있다.Dietary fiber is a prebiotics substance that is used by beneficial microorganisms in the large intestine to promote the growth or activity of microorganisms and thus exhibit a beneficial effect on the health of the host. As a prebiotics substance, blood insulin, glucose, It is known that it has an effect of suppressing an increase in concentration of triglyceride and cholesterol, decreasing the amount of food intake, inhibiting weight gain, decreasing body fat, improving constipation and improving the glucose tolerance of diabetic patients .
하지만, 서구화된 식습관의 도입과 영양의 과다섭취로 인해 식이섬유 섭취량이 감소하고 있으며, 1인당 하루 평균 식이섬유 추정 섭취량은 조사자에 따라 다소 차이가 있으나 20g 미만으로 잠정적 권장량에 미달하는 것으로 알려져 있다. 이와 같은 식이섬유 섭취량의 저하로 인해 과체중, 성인병 등의 발병률이 증가하여 국민의 건강에 심각한 위협이 되고 있다.However, the intake of dietary fiber is decreasing due to introduction of westernized diet and excessive nutrition, and the estimated daily intake of dietary fiber per person is slightly different according to the investigator, but it is less than 20g, which is less than the tentative recommended amount. Such a decrease in dietary fiber intake has increased the incidence of overweight, adult diseases and the like, posing a serious threat to public health.
한편, 보리, 율무, 콩, 조, 기장, 수수, 옥수수 등과 같은 잡곡은 쌀에 비해 열등한 작물로서 여겨져 왔으나, 쌀에 부족한 영양성분을 보충하는 역할을 할 뿐만 아니라, 성인병 예방에 필요한 식이섬유, 비타민, 미네랄 등이 쌀에 비해 약 2 내지 3배 정도 풍부하여 기능성 소재로써 가치가 높은 것으로 알려지면서, 최근 쌀소비 감소와 재고미 증가에 따른 문제점을 해결하고 농가소득을 증진시키기 위한 소득 작물로 주목받고 있다.On the other hand, grains such as barley, yulmu, soybean, japonica, millet, sorghum and corn have been regarded as inferior crops compared to rice, but they supplement nutrients that are insufficient for rice. In addition to dietary fiber, vitamins And minerals are abundant about 2 to 3 times as much as rice and are known to have high value as a functional material. Recently, attention has been paid to solve the problems caused by decrease in consumption of rice and increase in inventory, and to increase income of farm households have.
또한, 잡곡은 영양 공급적인 측면뿐만 아니라, 생체적 리듬과 신진대사 조절 또는 질병예방 등을 향상시키는 건강기능으로 초점이 전환되고 있으며, 이에 따라 기능적 측면의 식품의 소재로써 잡곡의 가치가 증가하고 있다.In addition, grains are being shifted to nutritional aspects as well as to health functions that improve the biological rhythm, metabolic control, or disease prevention, thereby increasing the value of grains as functional food ingredients .
하지만, 종래에는 대부분 잡곡을 밥에 섞어 먹는 혼반용으로 사용하고 있으며, 가공제품으로의 이용은 가공적성과 기호도 문제로 인하여 극히 제한적으로 이용되고 있는 실정으로, 식이섬유가 다량 함유된 귀리와 수수 등의 잡곡을 이용하여 빵이나 과자처럼 모든 사람들이 즐겨 먹을 수 있는 식품에 적용한다면 식이섬유가 풍부한 식품을 효율적으로 섭취할 수 있을 것으로 판단된다.However, conventionally, most of them are used for mixed rice, which is mixed with rice, and the use thereof as a processed product is extremely limited due to problems of processing suitability and preference. Thus, oats and sorghum containing a large amount of dietary fiber , It is considered that dietary fiber-rich foods can be efficiently consumed if they are applied to foods that can be enjoyed by all people, such as breads and cookies.
따라서, 식이섬유가 풍부한 잡곡섭취를 촉진하기 위해서는 소비자의 다양한 기호에 맞는 모양과 이화학적 특성을 갖춘 식품의 가공기술이 필요한 실정이다.Therefore, in order to promote the intake of dietary fiber-rich grains, it is necessary to develop a food processing technology having shape and physico-chemical characteristics suitable for various consumers' preferences.
본 발명의 발명자들은 난용성 곡물 소재가 장내 유익 미생물에 증식에 미치는 영향에 대한 상관관계에 대해 연구하던 중, 난용성 곡물을 팽화 처리한 다음 섭취하면 곡물 입자가 장내 유익 미생물이 서식하기 위한 공간 및 환경을 제공하고, 장내 식이섬유의 이용률이 증가되어 장내 유익 미생물 생육 증진효과를 발휘함을 확인하였고, 이와 더불어 식이섬유를 효과적으로 섭취할 수 있는 방법을 확립함으로써 본 발명을 완성하였다. The inventors of the present invention have been studying the correlation between the poorly soluble grain material and the effect on the proliferation of beneficial microorganisms in the intestines. When the insoluble grains are expanded and then ingested, the grain particles are separated into spaces for beneficial microorganisms The present invention has been accomplished by establishing a method for effectively feeding dietary fiber, and has found that the use of dietary fiber in the intestinal tract is enhanced to enhance the growth of beneficial microorganisms in the intestines.
따라서, 본 발명은 프로바이오틱스(probiotics) 및 프리바이틱스(prebiotics)의 유용한 효과를 동시에 나타낼 수 있는 팽화 곡물과자의 제조방법에 대한 기술 내용을 제공하고자 하는 것이다.Accordingly, the present invention is intended to provide a technical description of a method for producing expandable grain confectionery which can simultaneously exhibit useful effects of probiotics and prebiotics.
상기한 바와 같은 기술적 과제를 달성하기 위해서 본 발명은, (a) 곡물을 분쇄하여 곡물 입자를 제조하는 단계; (b) 곡물 입자를 건조하는 단계; (c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계; (d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및 (e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는 팽화 곡물과자의 제조방법을 제공한다. According to an aspect of the present invention, there is provided a method of manufacturing a grain grains comprising the steps of: (a) pulverizing grains to produce grain grains; (b) drying the grain particles; (c) expanding the dried grain particles to produce expanded grain particles; (d) pulverizing the expanded grain particles to produce a grain powder; And (e) molding the cereal powder to produce a confectionery cake.
또한, 본 발명은 상기에 기재된 방법으로 제조된 팽화 곡물과자를 제공한다.The present invention also provides expanded wheat flour made by the above-described method.
본 발명에 따른 팽화 곡물과자는 식이섬유 및 유효성분의 체내 흡수율이 현저히 증가되어 장내 유익 미생물의 생육을 효과적으로 증진시킬 수 있을 뿐만 아니라, 기호성 및 유효성분의 함량이 증진된 팽화 곡물과자의 제조방법을 확립하였으며, 본 발명의 제조방법으로 제조한 팽화 곡물과자는 비피도 박테리아 등과 같은 장내 유익 미생물인 프로바이오틱스의 생육을 증진시키는 프리바이오틱스 물질로서, 이를 섭취하게 되면 병원성균에 의한 장 감염을 예방하고, 장내 균총의 균형을 유지할 수 있을 뿐만 아니라, 발암억제, 콜레스테롤 감소, 면역증강 등의 우수한 효능을 가지므로 유용하게 이용할 수 있다.The expanded granular confection according to the present invention can remarkably increase the absorption rate of dietary fiber and active ingredient in the body to effectively enhance the growth of the beneficial microorganism in the intestines as well as to provide a method for producing the expanded granular confection having improved palatability and effective ingredient content The expanded cereal cake prepared by the production method of the present invention is a prebiotic substance which promotes the growth of probiotic, which is an intestinal beneficial microorganism such as Bifidobacterium. When ingested, prevent intestinal infection by pathogenic bacteria, It can be usefully used because it has excellent effects such as inhibition of carcinogenesis, reduction of cholesterol, and enhancement of immunity, as well as maintaining the balance of intestinal flora.
도 1은 장내 프로바이오틱 증진 난용성 곡물식이섬유 팽화 곡물과자의 제조공정을 나타낸 사진이다.
도 2는 곡물 추출물에서 배양한 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)의 활성이 증진됨을 나타낸 그래프이다.
도 3은 동결건조 곡물 열수추출물에서 배양한 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)의 활성이 증진됨을 나타낸 그래프이다.
도 4는 혼합곡물분말과 장내유익균 혼합물의 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다.
도 5는 3D 이미지로 구현한 혼합곡물과 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다.
도 6은 찰기장, 현미 및 귀리 각각의 분말 및 일정비율로 혼합된 곡물분말과 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다. Fig. 1 is a photograph showing a manufacturing process of a cooked grain confectionery fiber of the present invention.
Fig. 2 shows a comparison of four microorganisms in the intestines ( Lactobacillus acidophilus KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357).
Fig. 3 shows the results of analysis of four microorganisms in the intestines ( Lactobacillus acidophilus KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium bifidum < / RTI > KCTC 3357).
Fig. 4 is a CLSM image showing the ability of Lactobacillus acidophilus to adhere to a mixed grain powder and a mixture of beneficial bacteria in the intestinal tract.
FIG. 5 is a CLSM image showing the ability of mixed grains and Lactobacillus acidophilus to be adhered to 3D images.
FIG. 6 is a CLSM image showing the adherence of Lactobacillus acidophilus and grain powder mixed at a certain ratio in each of the barley, brown rice and oat.
이하, 본 발명을 상세히 설명하도록 한다.Hereinafter, the present invention will be described in detail.
본 발명은 (a) 곡물을 분쇄하여 곡물 입자를 제조하는 단계; (b) 곡물 입자를 건조하는 단계; (c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계; (d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및 (e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는 팽화 곡물과자의 제조방법을 제공한다(도 1). (A) grinding the grain to produce grain particles; (b) drying the grain particles; (c) expanding the dried grain particles to produce expanded grain particles; (d) pulverizing the expanded grain particles to produce a grain powder; And (e) molding the cereal powder to prepare a confectionery cake (Fig. 1).
상기 단계 (a)의 곡물은 보리, 수수, 귀리, 율무, 기장, 식용피, 좁쌀, 찹쌀, 현미, 백미로 이루어진 군으로부터 선택되는 1종 이상을 포함하는 곡물을 분쇄하여 곡물 입자를 제조하는 단계이다. Wherein the grain in step (a) is prepared by pulverizing grains containing at least one selected from the group consisting of barley, sorghum, oats, yulmu, millet, edible blood, millet, glutinous rice, to be.
상기 단계 (a)의 곡물은 전체 팽화 곡물과자 총중량 대비 50 내지 55 중량%를 첨가하는 것이 바람직하나 이에 한정하지 않는다.Preferably, the grain of step (a) is added in an amount of 50 to 55% by weight based on the total weight of the whole expanded grain cake, but is not limited thereto.
상기 곡물은 식이섬유의 포함함량이 높은 곡물을 선정하여 사용할 수 있으며, 보리, 수수, 귀리, 율무, 기장, 식용피, 좁쌀, 찹쌀, 현미 또는 백미 등과 같이 잡곡 및 쌀의 혼합물을 사용할 수 있으나, 바람직하게는, 조섬유의 포함함량이 7% 이상으로 높은 수수, 귀리, 율무, 식용피, 좁쌀, 기장, 찹쌀 또는 이들의 혼합물을 사용하여 장내 유익 미생물의 영양분이되는 프로바이오틱스 물질인 식이섬유를 다량 공급할 수 있다.The grain may be selected from cereals having a high content of dietary fiber and may be a mixture of cereals and rice such as barley, sorghum, oats, yulmu, millet, edible bloom, millet, glutinous rice, brown rice or white rice. Preferably, a large amount of dietary fiber, which is a probiotics substance which is a nutrient of the intestinal beneficial microorganism, is used by using a high content of crude fiber, oats, yulmu, edible blood, millet, millet, glutinous rice, .
상기 잡곡은 쌀밥에 부족한 영양성분 보충 역할 뿐만 아니라 성인병 예방에 필요한 식이섬유, 비타민, 미네랄 등이 쌀에 비해 대략 2 내지 3배 정도 풍부하여 기능성 소재로써 가치가 높다.The cereals are rich in dietary fiber, vitamins, and minerals, which are necessary for the prevention of adult diseases, as well as supplementing nutrients that are insufficient for rice, which is about 2 to 3 times more abundant than rice.
특히, 귀리는 7 내지 22%의 식이섬유와 3.8%의 베타-글루칸(β-glucan)이 함유되어 있어 지방 세포의 축적을 억제함으로써 체지방 형성을 감소시키고, HDL 콜레스테롤을 증가시켜 혈중 콜레스테롤의 수치를 감소시키며, 간에 콜레스테롤이 축적되는 것을 억제하며, 지방질의 소화기능을 저하하며, 지질 대사를 개선시켜 항비만 효과가 있다. 또한, 글로불린(globulin)은 다량 포함하고, 프롤라민의 함량은 낮으며, 라이신 등의 필수 아미노산하고, 팔미트산, 올레산, 리놀레익산 등의 지방산을 다량 포함하며, 비타민 B와 칼슘 등의 포함함량이 높아 우수한 곡물 소재로서, 생리활성 물질인 고분자 다당체가 다량 함유되어 있어 다양한 생리효능이 있고 식후 혈당상승억제 및 콜레스테롤 개선하는 효능을 가져 팽화 곡물과자 제조에 원료로 사용할 수 있다.In particular, oats contain 7 to 22% of dietary fiber and 3.8% of beta-glucan, thereby inhibiting accumulation of adipocytes, thereby reducing body fat formation, increasing HDL cholesterol, , Inhibits the accumulation of cholesterol in the liver, decreases the digestive function of lipids, and improves lipid metabolism. In addition, it contains a large amount of globulin, a low content of prolamin, a large amount of essential amino acids such as lysine, fatty acids such as palmitic acid, oleic acid and linoleic acid, and contains vitamin B and calcium It has excellent physicochemical properties because it contains a high amount of high molecular weight polysaccharide which is a physiologically active substance, and has an effect of suppressing postprandial increase of blood glucose and improving cholesterol. Thus, it can be used as a raw material for manufacturing expanded wheat confectionery.
상기 수수는 6 내지 8%의 식이섬유를 포함하며, 플라보노이드를 다량 포함하고, 탄닌, 페놀산, 안토시아닌, 피토스테롤, 폴리코사놀 등을 주요 성분으로 포함하여, 항암 효과, 면역기능 강화, 콜레스테롤 저하시키는 역할을 가져 팽화 곡물과자 제조에 원료로 사용할 수 있다.It contains 6 to 8% of dietary fiber and contains a large amount of flavonoids. It contains tannin, phenolic acid, anthocyanin, phytosterol, policosanol, etc. as its main components and acts as anticancer effect, immune function and cholesterol lowering It can be used as a raw material in the manufacture of expanded sweets.
상기 백미, 현미 등과 같은 쌀은 재배지역에 따라 다소 차이는 있으나, 백미의 경우, 가식부 100g당 75.5 g의 당질, 6.8 g의 단백질, 1.3 g의 지질 및 0.4 g의 조섬유를 포함하며, 회분을 대략 0.6 mg/100 g의 농도로 함유하고, 인, 칼슘, 마그네슘, 나트륨, 철분 등이 함유되어 팽화 곡물과자 제조에 이용할 수 있다. 또한, 쌀의 주된 단백질은 글루텔린(glutelin)계 오리제닌(oryzenin)을 포함하며, 소량의 글루불린과 알부민 등의 양질의 단백질을 포함한다.Rice such as white rice, brown rice and the like are slightly different depending on the cultivation area, but in case of white rice, 75.5 g of carbohydrate, 6.8 g of protein, 1.3 g of lipid and 0.4 g of crude fiber per 100 g of edible portion, 0.6 mg / 100 g, and contains phosphorus, calcium, magnesium, sodium, iron and the like and can be used for producing expanded wheat confectionery. In addition, the main protein of rice contains glutelin oryzenin, and it contains high quality proteins such as small amounts of glutibin and albumin.
상기 단계 (a)에서는 건조한 곡물 입자를 팽화시키는 단계로서, 곡물 입자는 조직이 치밀한 상태로 수축되어 있어, 섭취시 식이섬유와 유효성분의 효율적인 섭취가 곤란한 문제가 있다.The step (a) is a step of expanding the dried grain particles. The grain particles are shrunk in a dense state, and thus there is a problem in that efficient ingestion of the dietary fiber and the active ingredient is difficult at the time of ingestion.
이에 따라, 본 단계에서는 곡물 입자를 팽화시켜, 식이섬유와 유효성분의 생체 흡수율을 증가시킬 수 있을 뿐만 아니라, 곡물의 갖는 구수한 맛과 향을 더욱 증진시켜 기호성을 높이고, 각종 이취를 제거된 곡물 입자를 제조할 수 있다.Thus, in this step, the grain particles are expanded to increase the bio-absorption rate of the dietary fiber and the active ingredient. In addition, the taste and flavor of the grain can be further enhanced to enhance the palatability, Can be produced.
이를 위해, 상기 단계 (c)에서는 곡물 입자를 수증기 공급이 가능한 밀폐형 즉석 팽화장치에 공급하고, 수증기를 주입하면서 가열하여 곡물 입자의 조직 내부로 수증기가 확산되도록 하면서, 곡물 입자의 세포벽을 부분적으로 파쇄하고, 일정 비율로 조직이 부풀러 망상조직을 형성하도록 하여 곡물 입자의 팽화를 유도할 수 있다. For this, in the step (c), the grain particles are supplied to a sealed instant expanding device capable of supplying water vapor, and the water vapor is heated while injecting steam to diffuse the steam into the tissue of the grain grains, And induce the swelling of the grain particles by causing the tissue to form a bluller network at a certain rate.
상기와 같이 밀폐형 즉석 팽화장치를 이용하여 곡물 입자를 팽화시키는 팽화 공정은 220 내지 330℃의 온도에서 수행할 수 있으며, 상기 팽화공정의 온도가 230℃를 초과하는 경우, 곡물 입자에 포함된 유효 성분이 열 분해되어, 유효성분의 포함함량이 떨어지는 문제가 있고, 상기 팽화공정의 온도가 220℃ 미만인 경우, 상기 곡물 입자의 팽화효율이 떨어지고, 팽화시간이 증가하는 문제가 있으며, 압력을 조절하여 보다 원활히 곡물 입자를 팽화시킬 수 있다.The expansion process for expanding the grain particles using the closed instantaneous expansion device as described above can be performed at a temperature of 220 to 330 ° C. When the temperature of the expansion process exceeds 230 ° C., the effective ingredient There is a problem in that the content of the active ingredient is lowered. When the temperature of the expansion process is less than 220 캜, the expansion efficiency of the grain particles is lowered and the expansion time is increased. The grain particles can be smoothly expanded.
또는, 상기 곡물 입자를 스크류가 구비된 압출장치에 장입하고 압출하는 방법으로 곡물 입자를 팽화시킬 수 있다. 이와 같은 압출(extrusion) 공정은, 곡물 입자의 가공성을 개선할 수 있는 방법으로, 곡물에 압출공정을 적용하게 되면 곡물 입자에 포함된 전분, 단백질, 지질 성분들이 고온 상태에서 변형되어 점탄성을 갖게 되고, 수증기나 가스 등에 의해 부피가 증가하며 구조가 파괴되어 조직 재형성을 꾀할 수 있을 뿐만 아니라, 기계적 에너지에 의해 비교적 짧은 시간내에 팽화 곡물 입자를 제조할 수 있는 장점을 갖는다.Alternatively, the grains may be expanded by charging the grains into an extruder equipped with a screw and extruding the grains. Such an extrusion process is a method that can improve the processability of grain grains. When an extrusion process is applied to grains, the starch, protein, and lipid components contained in the grain grains are deformed at high temperature to become viscoelastic , The volume is increased due to steam or gas, the structure is destroyed and the structure can be reformed, and expanded grain particles can be produced in a relatively short time by mechanical energy.
이를 위해, 상기 압출공정으로 곡물 입자를 팽화시키기 위해서는, 건조한 곡물 입자를 특정 입자 크기를 갖도록 분쇄하여 팽화 공정을 수행할 수 있으며, 바람직하게는 30 내지 60 메쉬(mesh)의 입자크기를 갖는 곡물 분말을 제조한 후, 압출장치에 장입하여 압출공정을 수행하여 팽화 효율을 극대화시킬 수 있다.For this purpose, in order to expand the grain particles by the extrusion process, the dried grain particles may be pulverized to have a specific particle size to perform the expansion process. Preferably, the grain powder having a particle size of 30 to 60 mesh It is possible to maximize the expansion efficiency by charging it into the extrusion apparatus and performing the extrusion process.
상기와 같이 압출장치를 이용할 경우, 상기 팽화 공정은 바렐부의 온도를 90 내지 130℃로 조절하고, 투입속도를 고려하여 수분함량을 조절하는 방법으로 곡물 입자를 압출시킴에 따라, 곡물 입자에 팽화를 유도할 수 있으며, 이에 의해 식이섬유 및 유효성분의 감소가 극소화된 우수한 품질의 팽화 곡물 입자를 제조할 수 있다.In the case of using the extrusion apparatus as described above, the expansion process adjusts the water content in consideration of the feeding rate by controlling the temperature of the barrel portion at 90 to 130 ° C. and extruding the grain particles, So that it is possible to produce expanded grain particles of excellent quality with minimal reduction of dietary fiber and active ingredient.
상기와 같이 팽화가 유도된 곡물 입자는 곡물 입자의 세포벽이 부분적으로 파쇄된 상태로 곡물 입자에 포함된 식이섬유 및 유효성분의 생체 흡수율을 향상시킬 수 있을 뿐만 아니라, 팽화에 의해 곡물 자체가 갖는 구수한 맛과 향이 더욱 강해짐에 따라 기호성이 현저히 향상되게 된다.In the above-mentioned cereal grain in which expansion is induced, not only the biofeeding rate of the dietary fiber and the active ingredient contained in the cereal grain in the state where the cell wall of the grain grain is partially broken can be improved, but also the grain- As taste and flavor become stronger, palatability is remarkably improved.
특히, 상기와 같이 팽화처리한 팽화 곡물 입자는 1 내지 3의 팽화도를 가지며, 곡물 입자의 세포벽이 부분적으로 파쇄되어 수분흡착지수가 현저히 향상될 뿐만 아니라, 장내 유익균의 영양분인 식이섬유가 장내에서 다량 흡수되고, 장내 유익 미생물의 생육공간 및 환경을 조성하여 섭취자의 건강 및 면역력 향상에 기여할 수 있다.Particularly, the expandable grain particles as described above have a degree of puffing of 1 to 3, the cell walls of the grain particles are partially broken and the water absorption index is remarkably improved. In addition, the dietary fiber, which is the nutrient of the intestinal beneficial bacteria, It is absorbed in a large amount, and the growth space and environment of the beneficial microorganism in the intestines are formed, thereby contributing to improvement of health and immunity of the recipient.
상기 단계 (d)에서는 팽화시킨 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계로서, 본 단계에서는 상기 곡물 분말을 특정한 입자 크기를 갖는 분말 형태로 분쇄하여 후술할 단계에서 각종 첨가물과 혼합하여 다양한 형상 및 모양을 갖도록 성형됨으로써, 기호성이 높은 팽화 곡물과자를 제조할 수 있게 된다.In the step (d), the granulated powder is pulverized to produce a grain powder. In this step, the grain powder is pulverized into a powder having a specific particle size and then mixed with various additives in the following step, Shape, so that it is possible to produce expanded puffed cake having high palatability.
상기 단계 (e)에서는, 상기 팽화 곡물 분말을 성형하여 팽화 곡물과자를 제조하는 단계로서, 본 단계에서는 상기 팽화 곡물 분말을 포함하는 곡물 조성물을 제조하고, 제조한 곡물 조성물을 성형하여 다양한 크기 및 형상을 갖는 팽화 곡물과자를 제조할 수 있으며, 통상적인 분말을 이용한 과자의 제조방법이라면 제한받지 않고 사용할 수 있다.In the step (e), the expanded granular powder is molded to produce expanded granular confection. In this step, a grain composition containing the expanded granular powder is prepared, and the prepared grain composition is molded into various sizes and shapes Can be produced, and any method for producing confectionery using a conventional powder can be used without limitation.
상기 곡물 조성물은 팽화 곡물 분말 이외에도 다양한 맛과 향을 구현할 수 있도록, 다양한 첨가물을 첨가하여 제조할 수 있으며, 특히, 상기 첨가물로 시즈닝, 팥, 홍삼, 설탕, 물엿, 소르비톨 또는 정제염 등의 조미원료를 첨가하여 기호성이 더욱 향상된 팽화 곡물과자를 제조하도록 구성할 수 있다. 상기 시즈닝은 백설탕, 포도당, 유당, 탈지분유, 향신료, 코코아 분말, 메이플향, 천일염, 건생강분말 및 수크랄로스를 배합하여 추러스 맛을 낸것을 이용할 수 있다.The above-mentioned cereal composition can be prepared by adding various additives so as to realize various flavors and aromas in addition to the expanded grain powder. Especially, the seasoning raw materials such as seasoning, red bean, red ginseng, sugar, starch syrup, sorbitol, Can be added to prepare expanded wheat confectionery having further improved palatability. The seasoning may be a mixture of white sugar, glucose, lactose, skim milk powder, spice, cocoa powder, maple flavor, sun salt, dried ginger powder and sucralose to give a thrush flavor.
상기한 바와 같은 본 발명에 따른 팽화 곡물과자의 제조방법에 따르면, 곡물을 팽화시켜 팽화 곡물을 제조하고, 이를 곡물과자의 제조에 이용함으로써, 식이섬유 및 유효성분의 체내 흡수율이 현저히 증가되어 장내 유익 미생물의 생육을 효과적으로 증진시킬 수 있을 뿐만 아니라, 기호성 및 유효성분의 함량이 증진된 팽화 곡물과자를 제조할 수 있다.According to the method for producing expanded granular confection according to the present invention as described above, the expansion rate of the dietary fiber and the active ingredient in the body is remarkably increased by producing the expanded granules by expanding the cereals to prepare the expanded granules, It is possible not only to effectively enhance the growth of microorganisms but also to produce expanded wheat confection having increased palatability and effective ingredient content.
또한, 쌀 및 잡곡의 소비를 촉진시키기 위한 방편으로 활용되어 농가소득 증진에 기여할 수 있다.In addition, it can be used as a means to promote the consumption of rice and grains, thereby contributing to the increase of farm income.
상기 방법으로 제조한 팽화 곡물과자는 비피도 박테리아 등과 같은 장내 유익 미생물인 프로바이오틱스의 생육을 증진시키는 프리바이오틱스 물질로서, 이를 섭취하게 되면 병원성균에 의한 장 감염을 예방하고, 장내 균총의 균형을 유지할 수 있을 뿐만 아니라, 발암억제, 콜레스테롤 감소, 면역증강 등의 우수한 효능을 갖는다.The expanded cereal cake prepared by the above method is a prebiotics substance that promotes the growth of probiotic, a beneficial microorganism such as Bifidobacterium. When ingested, prevent intestinal infections caused by pathogenic bacteria and maintain the balance of intestinal microflora But also has excellent effects such as inhibition of carcinogenesis, reduction of cholesterol, and immunity enhancement.
따라서, 본 발명은 상기에 기재된 방법으로 제조된 팽화 곡물과자를 제공한다.Accordingly, the present invention provides expanded wheat flour made by the above-described method.
상기 팽화 곡물과자는 팽화 곡물을 포함하여 장내에서 곡물에 포함된 식이섬유의 흡수율을 증진시키고, 식이섬유를 장내 유익 미생물이 용이하게 섭취할 수 있어 장내 유익 미생물의 생육을 증진시키는 효과를 갖는다.The expanded cereal cake contains expanded granules to enhance the absorption rate of dietary fiber contained in the cereals in the intestines, and the dietary fiber can be easily ingested by intestinal beneficial microorganisms, thereby enhancing the growth of intestinal beneficial microorganisms.
상기 장내 유익 미생물은 유산균 등과 같은 장내 유익균이라면, 제한되지 않으나, 특히, 락토바실러스 에시도필러스(Lactobacillus acidophillus), 락토바실러스 델브룩키 스페이시즈 락티스(Lactobacillus delbrueckii subsp. lactis), 스테렙토코커스 살리바리어스 스페이시즈 써모필러스(Streptococcus salivarius subsp. thermophilus), 비피도박테리움 비피덤(Bifidobacterium bifidum) 등과 같은 장내 유익 미생물의 생육을 효과적으로 증진시키는 효과를 갖는다.The intestinal beneficial microorganism is not particularly limited if it is beneficial bacteria in the intestines such as lactic acid bacteria and the like. In particular, Lactobacillus acidophilus , Lactobacillus delbrueckii subsp. Lactis , Streptococcus salivarius ( Streptococcus salivarius) subsp . thermophilus), Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum ), and the like.
이하, 본 발명을 실시예를 들어 더욱 상세히 설명하도록 한다.Hereinafter, the present invention will be described in more detail with reference to examples.
제시된 실시예는 본 발명의 구체적인 예시일 뿐이며, 본 발명의 범위를 제한하기 위한 것은 아니다.The embodiments presented are only a concrete example of the present invention and are not intended to limit the scope of the present invention.
<< 실시예Example 1> 팽화 곡물과자의 제조 방법 1> Manufacturing Method of Expanded Grain Sweets
난곡성 곡물 유래 섬유소를 프리바이오틱스(Prebiotics) 소재로 활용한 팽화 곡물과자를 제조하였다. We have produced expanded cereal sweets using pre - fermented cereal - derived fiber as prebiotics.
구체적으로, 곡물(귀리, 현미, 백미 및 옥수수)을 하기 표 1에 기재된 배합비로 볼밀링 장치에 넣어 분쇄하여 30 내지 60 메쉬(mesh)의 평균입자 크기를 갖는 곡물 분말을 제조하였다. 후술할 압출공정에서 곡물 분말의 투입속도를 고려하여 곡물 분말의 수분함량을 30%가 되도록 조절하였고, 4℃에서 24시간 동안 평형화시킨 후 사용하였다.Specifically, cereals (oats, brown rice, white rice, and corn) were pulverized in a ball mill apparatus at the blending ratios shown in Table 1 below to prepare grain powders having an average grain size of 30 to 60 mesh. In the extrusion process described below, the moisture content of the grain powder was adjusted to 30% in consideration of the feeding rate of the grain powder, and the mixture was used after being equilibrated at 4 ° C for 24 hours.
제조한 곡물 분말을 압출하여 곡물 분말을 팽화시켰으며, 팽화는 곡물 분말을 쌍축압출성형기[인천기계공업(주)]에 장입하여 압출공정을 수행하여 곡물과자를 제조하였다. 쌍축압출성형기는 동방향 스크류가 구비되고, 내부직경이 31.2 mm인 바렐 4개가 구비되어 17.3 미들-샤프트(middle-shaft)를 유지할 수 있는 것을 사용하였고, 150 rpm으로 회전속도를 고정하고, 바렐Ⅱ의 온도는 90℃, 바렐Ⅲ의 온도는 120℃, 바렐 Ⅳ의 온도는 130℃로 설정하여 압출성형공정을 수행하였다.The grain powder was extruded to expand the grain powder. The granulated powder was charged into a biaxial extrusion extruder (Incheon Machinery Industry Co., Ltd.) and extruded to produce cereal sweets. The biaxial extrusion extruder was equipped with a screw in the same direction and equipped with four barrels having an inner diameter of 31.2 mm and capable of maintaining a middle shaft of 17.3. The rotating speed was fixed at 150 rpm, The extrusion molding process was performed by setting the temperature of the barrel III to 90 ° C, the temperature of the barrel III to 120 ° C, and the temperature of the barrel IV to 130 ° C.
<< 실시예Example 2> 최적 2> Optimal 프리바이오틱스Prebiotics (( prebioticsprebiotics ) 소재 선정) Material selection
<2-1> 곡물 <2-1> Grain 열수Heat number 추출물을 이용한 장내미생물 증진 활성 분석 Analysis of Intestinal Microbial Enhancement Activity Using Extracts
상기 실시예 1에서 제조한 팽화 곡물과자를 위한 최적 프리바이오틱스 소재를 선정하기 위해서, 난용성 유기농 소재인 잡곡과 쌀을 포함하는 곡물이 장내 유익 미생물 및 유산균의 증식에 미치는 영향을 분석하였다.In order to select the optimum prebiotic material for the expanded pastry cake prepared in Example 1, the effect of the grain containing insoluble organic material, such as grain and rice, on the proliferation of beneficial microorganisms and lactic acid bacteria in the intestines was analyzed.
구체적으로, 곡물(귀리, 백미, 현미 및 옥수수)을 난용성 유기농 소재로 선정하여 사용하였으며, 상기 곡물을 분쇄기(DA-10000G, Hanil, Seoul, Korea)를 사용하여 100 mesh size 이하로 분쇄한 분말 각각을 증류수에 첨가하여 10분간 혼합한 후, 원심분리(4℃, 13,000 rpm, 10분)하고, 0.2 ㎛ 공경 여과지를 이용해 여과하여 식이섬유 추출액을 제조하였다. 제조한 식이섬유 추출액이 장내 유익 미생물 및 유산균은 미쯔오카 방법(Mitsuoka method)에 의해 인체에서 분리한 장내 유익 미생물 및 발효식품에서 분리한 유산균에서 탐색한 균주로, 장내 유익 미생물 및 유산균을 각각 RCM 배지 및 MRS 배지에 접종한 후, 배양하고, 배양 후 50%(v/v) 글리세롤을 배양액에 첨가하여 70℃ 이하의 초저온냉장고(deep freezer)에 보관하였다. 상기 장내 유익 미생물 및 유산균을 사용 전 3회 이상 계대 배양 후 활성화하여 사용하였다.Specifically, grains (oats, white rice, brown rice, and corn) were selected as poorly soluble organic materials. The grains were pulverized to a size of 100 mesh or less using a pulverizer (DA-10000G, Hanil, Seoul, Korea) Each was added to distilled water, mixed for 10 minutes, centrifuged (4 ° C, 13,000 rpm, 10 minutes), and filtered through a 0.2 μm pore filter paper to prepare a dietary fiber extract. The beneficial microorganism and lactic acid bacterium produced in the intestinal tract were cultured in the intestinal beneficial microorganism isolated from the human body by the Mitsuoka method and in the lactic acid bacteria isolated from the fermented food. The intestinal beneficial microorganism and the lactic acid bacteria were cultured in RCM medium And MRS medium. After the culture, 50% (v / v) glycerol was added to the culture solution and stored in a deep freezer at 70 ° C or lower. The intestinal beneficial microorganism and the lactic acid bacteria were activated by subculturing three times or more before use.
상기 장내 유익 미생물 및 유산균 2.5 mL을 4.25 mL의 RCM 배지 및 MRS 배지에 각각 접종한 후, 상기 곡물에서 추출한 식이섬유 추출액 5mL씩을 가해 37℃에서 72시간 동안 진탕배양한 후, 흡광도 측정장치(spectrophotometer)로 660nm 파장의 빛에서 배양시간(0, 4h, 8h, 12h, 16h, 20h, 24h, 48h, 72h)에 따른 흡광도 및 생균수 변화를 측정하는 방법으로 곡물이 증식에 미치는 영향을 분석하였다. 이때, 대조군으로는 곡물에서 추출한 식이섬유 추출액 대신 5mL의 DMSO(30%)를 첨가하여 동일하게 측정하였으며, 흡광도 및 생균수를 측정하였다.2.5 mL of the intestinal beneficial microorganism and the lactic acid bacteria were inoculated into 4.25 mL of RCM medium and MRS medium, and 5 mL of the fiber extract extracted from the grain was added and incubated at 37 ° C for 72 hours with shaking. The effect of grain size on growth was investigated by measuring the absorbance and the number of viable cells according to incubation time (0, 4h, 8h, 12h, 16h, 20h, 24h, 48h, 72h) in 660nm wavelength light. As a control, 5 mL of DMSO (30%) was added to the same amount of fiber extracts extracted from cereals, and the absorbance and viable cell count were measured.
그 결과, 대조군에 비해 각각의 곡물 분말의 추출물을 첨가한 배지에서는 흡광도가 현저히 증가하는 것을 확인할 수 있었고, 생균수가 30% 이상 증가하는 것을 확인하였다.As a result, it was confirmed that the absorbance of the medium containing the extract of each grain powder was significantly increased and the number of viable cells was increased by 30% or more as compared with the control group.
(CFU/mL)Control group 1 )
(CFU / mL)
(CFU/mL)Grain powder 2 )
(CFU / mL)
subsp. lactis Lactobacillus delbrueckii
subsp. lactis
subsp. thermophilus Streptococcus salivarius
subsp . thermophilus
1) 대조군: 50%(w/v) MRS broth + 50% d 1) Control group: 50% (w / v) MRS broth + 50% d
2)곡물분말 : 50%(w/v) MRS broth + 50% 곡물 분말의 추출물 2) Grain powder: 50% (w / v) extract of MRS broth + 50% grain powder
3)성장률: 곡물분말의 생존 세포 수/대조군의 생존 세포 수 3) Growth rate: Number of viable cells in cereal powder / Number of viable cells in control
<2-2> 곡물 <2-2> Grain 열수Heat number 추출물을 이용한 장내미생물 증진 활성 분석 Analysis of Intestinal Microbial Enhancement Activity Using Extracts
곡물의 열수 추출물이 장내 유익 미생물의 생육에 미치는 영향을 분석하기 위해서, 곡물(귀리, 찰보리, 율무, 찰기장, 찰수수 및 현미)을 증류수에 투입하고, 55℃로 24시간 동안 가열하여 곡물 열수추출물을 제조하였으며, 제조한 열수추출물을 공경 5㎛의 여과지로 여과하였다. 한국생명공학연구원 생물자원센터에서 분양받아 활성화시킨 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)을 37℃에서 24시간 전배양 후, 3회 계대 배양하여 균주를 활성시킨 다음 계대배양 된 균주 5%(105 CFU/mL 농도)를 각각의 50% 곡물 열수 추출물에 접종하고50% MRS broth 혼합액에 분주하고, 48시간 배양시킨 후 시간 별(4h,8h,12h,18h,24h,36h,48h)로 흡광광도계를 이용하여 흡광도를 측정하여 그로스커브를 작성하여 배양시간별 흡광도 변화를 측정하였다In order to analyze the effect of hot water extract of cereals on the growth of beneficial microorganisms in the intestines, the cereals (oats, chrysanthemum, chrysanthemum, chrysanthemum, chickens and brown rice) were added to distilled water and heated for 24 hours at 55 ° C, The resulting hot-water extract was filtered through filter paper having a pore size of 5 탆. Four live microorganisms ( Lactobacillus acidophilus KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357) was cultured at 37 ° C for 24 hours, and subcultured three times to activate the strain. Subsequently, 5% (10 5 CFU / mL) of subcultured strain was added to each 50% And cultured for 48 hours. Then, the absorbance was measured using a spectrophotometer with time (4 h, 8 h, 12 h, 18 h, 24 h, 36 h, 48 h) The change in absorbance over time was measured
그 결과, 상기 곡물 추출물에서 배양한 장내 유익 미생물 4종 모두 대조군에 비해 유의적인 생육 증진 효과가 있는 것을 확인하였다(도 2).As a result, it was confirmed that all of the four beneficial microorganisms cultivated in the above-mentioned grain extract had a significant growth enhancement effect as compared with the control (Fig. 2).
<2-3> 동결건조 곡물 <2-3> Freeze-dried cereal 열수추출물을Hot water extract 이용한 장내미생물 증진 활성 분석 Analysis of intestinal microbial enhancing activity
동결건조한 곡물의 열수추출물이 장내 유익 미생물에 생육에 미치는 영향을 분석하였다.The effect of hot - water extract of freeze - dried grains on the growth of beneficial microorganisms in the intestines was analyzed.
구체적으로, 동결건조한 곡물(귀리, 찰보리, 율무, 찰기장, 찰수수 및 현미)의 열수추출물에서 생육하는 장내 유익 미생물의 성장곡선(growth curve)을 도출하였으며, 동결건조한 곡물 각각의 열수추출물을 제조하고, 제조한 열수 추출물을 감압농축기로 농축한 후, 동결건조기로 건조하여 50 mg/mL의 농도를 갖는 동결건조 곡물의 열수추출물을 제조하였다. 제조한 열수추출물을 배양 배지로 이용하여 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)을 접종하여 배양하였으며, 각각의 장내 유익 미생물이 증식하는 성장 곡선을 수득하였다.Specifically, the growth curves of beneficial microorganisms in the hot-water extracts of lyophilized grains (oat, chrysanthemum, yulmu, barley, chrysanthemum and rice) were obtained, The prepared hot water extract was concentrated with a vacuum concentrator and then dried with a freeze dryer to prepare a hot-water extract of freeze-dried cereal grains having a concentration of 50 mg / mL. Using the prepared hot water extract as a culture medium, four microorganisms ( Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357) were inoculated and cultured, and a growth curve in which the intestinal beneficial microorganisms proliferated was obtained.
그 결과, 락토바실러스 에시도필러스(Lactobacillus acidophillus KCTC 3164)는 찰보리, 율무, 귀리 등의 곡물 열수추출물에서 가장 높은 생육 증진효과를 보였으며, 현미를 제외한 다른 곡물에서 모드 유의적인 생육 증진효과를 나타내었다. 락토바실러스 델브룩키 스페이시즈 락티스(Lactobacillus delbrueckii subsp. lactis KCTC 3636)는 율무에서 가장 높은 증진효과를 갖는 것으로 확인되었고, 귀리 등 기타 곡물에서 유의적인 생육 증진효과를 나타낸다는 사실을 확인할 수 있었다. 스테렙토코커스 살리바리어스 스페이시즈 써모필러스(Streptococcus salivarius subsp. thermophilus KCTC 3658)의 경우에는 찰보리와 찰수수에서 가장 높은 생육 증진효과를 나타내었고, 귀리 등 다른 곡물에서도 생육 증진 효과를 나타내었다. 비피도박테리움 비피덤(Bifidobacterium bifidum KCTC 3357)의 경우에는 찰보리, 귀리, 찰수수에서 높은 증진효과를 나타내었고, 다른 곡물 모두 유의한 장내유익균 생육증진 효과를 보이는 것을 확인하였다(도 3).As a result, Lactobacillus acidophilus KCTC 3164 showed the highest growth promoting effect in the hydrothermal extracts of the grains such as paulchiae, yulmu, and oat, and showed a significant growth promoting effect in other grains except brown rice . Lactobacillus delbrueckii subsp. Lactis KCTC 3636) was found to have the highest enhancing effect in yulmu and showed significant growth enhancement effect in oats and other grains. Streptococcus salivarius subsp . Thermophilus < RTI ID = 0.0 & gt ; KCTC 3658) showed the highest growth promoting effect in the paddy field, and the growth promotion effect was also shown in other grains such as oats. Bifidobacterium Bifidobacterium bonus (Bifidobacterium bifidum KCTC 3357) showed a significant increase in palatability, oat, and number of wastes, and that the other grains showed significant growth promoting effect on the intestinal beneficial bacteria (FIG. 3).
상기와 같은 결과를 통해서, 상기 곡물 소재를 활용하면, 식이섬유를 고농도로 함유하면서 장내유익균의 생육을 증진시킬 수 있는 팽화식품 소재를 제조할 수 있음을 확인할 수 있었으며, 이를 통해 프리바이오틱스 소재를 특정 지을 수 있었다.As a result, it was confirmed that, by using the above-mentioned grain materials, it is possible to produce a puffed food material which can contain a high concentration of dietary fiber and enhance the growth of beneficial bacteria in the intestines. I was able to build it.
<< 실시예Example 3> 장내미생물 거동 모델 분석 3> Analysis of intestinal microbial behavior model
<3-1> <3-1> 프리바이오틱스Prebiotics 곡물분말과 Grain powder and 장내유익균간의Between the beneficial bacteria in the intestines 흡착도 분석 Adsorption analysis
곡물 분말에서 장내 유익 미생물이 나타내는 거동을 분석하기 위해서, 공초점 주사 레이져 현미경(Confocal laser Scanning Microscopy, CLSM)을 이용하여 프리바이오틱스(Prebiotics) 곡물소재의 영상 이미지를 수득하였으며, 곡물분말(귀리, 현미, 백미 및 옥수수)에 장내 유익 미생물인 락토바실러스 에시도필러스(Lactobacillus acidophillus KCTC 3164)을 접종하여 수수 분말에 대한 장내 유익 미생물의 부착능을 확인하였다.To analyze the behavior of the beneficial microorganisms in the intestinal flour, an image of Prebiotics cereal material was obtained using Confocal laser Scanning Microscopy (CLSM), and the grain powder (oats, Brown rice, white rice and corn) were inoculated with lactobacillus acidophilus ( Lactobacillus acidophilus KCTC 3164), which is an intestinal beneficial microorganism, to confirm the ability of the intestinal beneficial microorganism to adhere to the powder.
그 결과, 곡물 분말이 장내에서 장내 유익 미생물의 서식을 위한 공간 및 환경을 제공하여 장내 유익 미생물의 증식 및 생육에 도움을 줄 수 있을 것으로 판단되었다(도 4).As a result, it was judged that the grain powder could provide the space and environment for the microorganism in the intestines to help the growth and growth of beneficial microorganisms in the intestines (Fig. 4).
<3-2> 3D 이미지로 구현한 <3-2> Implemented with 3D image 프리바이오틱스Prebiotics 곡물분말과 Grain powder and 장내유익균간의Between the beneficial bacteria in the intestines 흡착도 분석 Adsorption analysis
곡물(귀리, 현미, 백미 및 옥수수)에서 장내 유익 미생물이 나타내는 거동을 분석하기 위해서, 장내 유익 미생물의 CLSM 3차원 이미지를 수득하였다. A CLSM three-dimensional image of intestinal beneficial microorganisms was obtained in order to analyze the behavior of the beneficial microorganisms in the intestines in the cereals (oats, brown rice, white rice and corn).
구체적으로, CLSM 3D 이미지 분석을 위한 최적 조건을 설정하고, 프리바이오틱스 곡물의 장내 유익 미생물 흡착도를 분석하였으며, 장내 유익 미생물을 24시간 동안 배양한 후, 4,000 rpm으로 5분간 원심분리하여, 상층액과 침전균체를 분리하였다. 분리한 균체를 PBS 완충용액(PBS buffer solution)으로 1회 세척한 후, 0.03% 형광이소티오시아네이트(fluorescein isothiocyanate, FITC)로 10분 동안 암실에서 염색하였으며, 염색한 후, PBS 완충용액으로 3회 세척한 후, CLSM으로 확인하였다.Specifically, optimal conditions for CLSM 3D image analysis were set up, and prebiotic grains were analyzed for beneficial microorganism adsorption, and the intestinal beneficial microorganisms were cultured for 24 hours, centrifuged at 4,000 rpm for 5 minutes, And the precipitated cells were separated. The cells were washed once with PBS buffer solution and stained with 0.03% fluorescein isothiocyanate (FITC) for 10 min in the dark. After staining, the cells were washed with PBS buffer solution After washing once, it was confirmed by CLSM.
그 결과, 곡물 분말이 장내에서 장내 유익 미생물의 서식을 위한 공간 및 환경을 제공하여 장내 유익 미생물의 증식 및 생육에 도움을 줄 수 있을 것으로 판단되었다(도 5).As a result, it was judged that the grain powder could provide a space and an environment for the microorganism in the intestines to support the growth and growth of beneficial microorganisms in the intestines (Fig. 5).
<3-3> <3-3> 프리바이오틱스Prebiotics 곡물분말과 장내 유익 미생물의 흡착도 분석 Adsorption analysis of grain powder and intestinal beneficial microorganisms
프리바이오틱스 혼합곡물분말(귀리, 현미, 백미 및 옥수수)과 장내 유익 미생물의 흡착도 확인하기 위해서, 곡물분말(귀리, 현미, 백미 및 옥수수), 현미, 귀리 분말 및 찰기장과 장내 유익 미생물을 실험을 통해 정해진 적정 형광염료와 농도를 이용하여 염색 후 CLSM을 이용하였으며, 곡물 분말과 균주의 혼합시 곡물의 자가형광이 넓은 파장대에서 강한 강도를 나타냄으로 균주와 구분의 명확하지 않아 DIC 필터(differential interference contrast filter)를 사용하여 상을 관찰하였고, 균주는 0.03% FITC를 사용하여 염색 후 곡물분말과 혼합하여 관찰하였다. To check the adsorption of prebiotics mixed grain powder (oats, brown rice, white rice and corn) and microorganisms beneficial for the intestines, we examined the grain powder (oat, brown rice, white rice and corn), brown rice, oat powder, CLSM was used after staining with the appropriate fluorescent dyes and concentrations determined by the DIC filter and the intensity of autofluorescence of grains was high at wide wavelength band when the grain powder and strain were mixed. contrast filter). The strain was stained with 0.03% FITC and mixed with grain powder.
그 결과, 장내 유익 미생물이 귀리와 혼합곡물분말(귀리, 현미, 백미 및 옥수수)의 주위로 부착되어 장내 유익 미생물이 곡물의 표면에 부착되는 곡물의 부착능이 찰기장 부착능보다 뛰어난 것을 확인하였으며, 혼합곡물분말이 장내에서 장내 유익 미생물의 서식을 위한 공간과 환경을 제공하여 장내미생물의 증식에 도움을 주는 것을 확인하였다(도 6).As a result, it was confirmed that the beneficial microorganisms in the intestines were adhered to oats and mixed grain powder (oats, brown rice, white rice and corn), and that the adhesion performance of the cereals having beneficial microorganisms in the intestines was superior to the sticky ability It was confirmed that the grain powder contributes to the growth of intestinal microorganisms by providing a space and environment for the microorganism in the intestines to form beneficial microorganisms (Fig. 6).
<< 실시예Example 4> 난용성 4> poor availability 프로바이오틱스Probiotics 팽화 곡물 과자의 특성 분석 Characterization of expanded wheat confection
<4-1> 압출 성형한 잡곡의 특성 분석<4-1> Characterization of Extruded Grains
압출 성형하여 팽화시킨 잡곡의 팽화도를 측정하여 팽화 효율을 분석하였다. The degree of expansion of the granules expanded by extrusion molding was measured to analyze the expansion efficiency.
하기 표 3에 나타난 바와 같이, 압출 성형하여 제조한 곡물의 팽화도는 1.70 내지 2.23의 범위에 속하였으며, 율무가 2.23으로 가장 높고, 찰보리가 1.74으로 가장 낮은 팽화도 값을 나타내었다. As shown in the following Table 3, the degree of puffing of the grains prepared by extrusion molding ranged from 1.70 to 2.23, The highest degree of bulgeing was found at 2.23 and the lowest value was 1.74.
<4-2> 압출성형 잡곡의 수분용해지수 및 수분 흡착지수 분석<4-2> Analysis of water solubility index and moisture adsorption index of extruded granules
압출성형하여 제조한 곡물의 수분용해지수 및 수분 흡착지수를 분석하였다. The water solubility index and moisture adsorption index of grains prepared by extrusion molding were analyzed.
하기 표 4에 나타낸 바와 같이, 압출성형하여 제조한 곡물의 수분용해지수 및 수분흡착지수는 팽화에 의해 현저히 증가하는 것으로 확인되었으며, 수분흡착지수는 보리가 가장 높은 것으로 나타났고, 특히, 현미는 대조군이 갖는 0.22 보다 팽화 후 3.37%로 현저히 증가하는 것으로 확인되었다. As shown in Table 4 below, it was found that the water solubility index and moisture adsorption index of cereals prepared by extrusion molding were significantly increased by puffing, and the moisture adsorption index was the highest in barley, and in particular, Was significantly increased to 3.37% after 0.22.
Experimental material
<< 실시예Example 5> 팽화 곡물과자의 관능평가 5> Sensory Evaluation of Swollen Grain Sweets
실시예 1에 따른 방법으로 제조하여 팽화 곡물과자의 관능평가는 식품을 전공하는 대학생 20명을 선정하여 수행하였다. The sensory evaluation of the swollen cereal cake prepared by the method according to Example 1 was performed by selecting 20 college students majoring in food.
관능평가는 곡물과자의 맛, 색, 씹힘성, 전반적인 기호도에 대한 특성을 5점 척도법으로 평가하여 '매우 나쁘다'를 1점, '매우 좋다'를 5점으로 1 내지 5점의 범위에서 점수를 부여하여 선호도가 높을수록 높은 점수를 주도록 설정하여 수행하였다. 또한, 시판되고 있는 A 곡물과자를 대조군으로 하여 제조한 팽화 곡물과자와 비교하여 관능평가를 수행하였고, 그 결과를 하기 표 5에 나타내었다(단, 하기 표 5에서 1은 대단히 싫어한다, 2는 싫어한다, 3은 보통이다, 4는 좋아한다, 5는 매우 좋아한다를 나타냄).The sensory evaluation was performed by evaluating the characteristics of flavor, color, chewiness and general preference of the cereal sweets by the 5-point scale method. The score was given in the range of 1 to 5 with 1 point for "very bad" and 5 points for "very good" The higher the preference, the higher the score was set. In addition, the sensory evaluation was carried out in comparison with the expanded wheat confectionery prepared by using commercially available A grain confectionery as a control group, and the results are shown in Table 5 (note that in Table 5, 1 is very displeased, Dislikes, 3 is common, 4 likes, 5 indicates very much).
그 결과, 상기 표 5에 나타낸 바와 같이 팽화 곡물과자의 관능평가를 수행한 결과, 색은 3.35±0.22으로 확인되었고, 맛은 3.21±0.62으로 확인되었으며, 조직감은 3.23±0.33으로 확인되었고, 향은 3.30±0.65으로 확인되어, 대조군인 A 곡물과자에 비해 관능평가 결과가 우수한 것으로 조사되었으며, 전체적인 기호도에서도 4.20±0.64로 대조군 곡물과자에 비해 우수한 것으로 확인되었다. As a result, as shown in Table 5, the sensory evaluation of the expanded confectionery cake showed that the color was 3.35 ± 0.22, the taste was 3.21 ± 0.62, the texture was 3.23 ± 0.33, 3.30 ± 0.65. It was found that the sensory evaluation was superior to that of control A, and the overall acceptability was 4.20 ± 0.64.
통상적으로, 제품에 있어 평점 3.5 이상이면 제품 개발에 우수함을 보이는데, 제조한 팽화 곡물과자는 4.0점을 웃도는 평점으로 소비자의 기호도에 적합하다는 사실을 확인할 수 있었다.Generally, a product with a rating of 3.5 or higher shows superiority in product development, and the manufactured expanded confectionery has a score of 4.0 or more, which is suitable for consumers' preference.
Claims (6)
(b) 곡물 입자를 건조하는 단계;
(c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계;
(d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및
(e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는 팽화 곡물과자의 제조방법.(a) milling the grain to produce grain particles;
(b) drying the grain particles;
(c) expanding the dried grain particles to produce expanded grain particles;
(d) pulverizing the expanded grain particles to produce a grain powder; And
(e) molding the cereal powder to produce cookies.
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