KR102433052B1 - Method for nonsoluble dietary fiber manufacturing puffed snack with improving proliferation of probiotics in intestine - Google Patents
Method for nonsoluble dietary fiber manufacturing puffed snack with improving proliferation of probiotics in intestine Download PDFInfo
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- KR102433052B1 KR102433052B1 KR1020170079606A KR20170079606A KR102433052B1 KR 102433052 B1 KR102433052 B1 KR 102433052B1 KR 1020170079606 A KR1020170079606 A KR 1020170079606A KR 20170079606 A KR20170079606 A KR 20170079606A KR 102433052 B1 KR102433052 B1 KR 102433052B1
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- grain
- intestinal
- growth
- confectionery
- beneficial
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/3262—Foods, ingredients or supplements having a functional effect on health having an effect on blood cholesterol
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
Abstract
본 발명은 장내 프로바이오틱을 증진하는 팽화 곡물 과자 및 이의 제조방법을 제공하는 것으로, 본 발명에 따른 팽화 곡물과자는 식이섬유 및 유효성분의 체내 흡수율이 현저히 증가되어 장내 유익 미생물의 생육을 효과적으로 증진시킬 수 있을 뿐만 아니라, 기호성 및 유효성분의 함량이 증진된 팽화 곡물과자의 제조방법을 확립하였으며, 본 발명의 제조방법으로 제조한 팽화 곡물과자는 비피도 박테리아 등과 같은 장내 유익 미생물인 프로바이오틱스의 생육을 증진시키는 프리바이오틱스 물질로서, 이를 섭취하게 되면 병원성균에 의한 장 감염을 예방하고, 장내 균총의 균형을 유지할 수 있을 뿐만 아니라, 발암억제, 콜레스테롤 감소, 면역증강 등의 우수한 효능을 가지므로 유용하게 이용할 수 있다.The present invention provides a puffed grain confectionery that promotes intestinal probiotics and a manufacturing method thereof, wherein the puffed grain confectionery according to the present invention significantly increases the absorption rate of dietary fiber and active ingredients in the body, thereby effectively promoting the growth of beneficial microorganisms in the intestine In addition, a method for producing puffed grain cookies with improved palatability and content of active ingredients has been established, and the puffed grain cookies prepared by the manufacturing method of the present invention promotes the growth of probiotics, which are beneficial intestinal microorganisms such as Bifidobacteria. As a prebiotic substance that promotes enhancement, when consumed, it can prevent intestinal infections caused by pathogenic bacteria and maintain the balance of intestinal flora, and is useful because it has excellent effects such as cancer suppression, cholesterol reduction, and immunity enhancement. Available.
Description
본 발명은 장내 프로바이오틱을 증진하는 팽화 곡물 과자 및 이의 제조방법을 제공하는 것이다. The present invention provides a puffed grain confectionery that promotes intestinal probiotics and a method for preparing the same.
식이섬유(dietary fiber)는 대장 내 유익 미생물에 의해 이용되어 미생물의 생육이나 활성을 촉진함으로써 숙주 건강에 좋은 효과를 나타내게 하는 비소화성 식품성분인 프리바이오틱스(prebiotics) 물질로서, 혈중 인슐린, 글루코오스, 중성지방, 콜레스테롤 등의 농도 상승을 억제하고, 식사 섭취량을 감소시키며, 체중 증가를 억제하고, 체지방을 감소시키는 효과가 있을 뿐만 아니라, 변비 개선과 당뇨병 환자의 내당능을 개선하는 효과가 있는 것으로 알려져 있다.Dietary fiber is a non-digestible food ingredient that is used by beneficial microorganisms in the large intestine to promote the growth or activity of microorganisms, thereby exhibiting a good effect on host health. It is known to have the effect of suppressing the increase in the concentration of triglycerides, cholesterol, etc., reducing the meal intake, suppressing weight gain, and reducing body fat, as well as improving constipation and glucose tolerance in diabetic patients. .
하지만, 서구화된 식습관의 도입과 영양의 과다섭취로 인해 식이섬유 섭취량이 감소하고 있으며, 1인당 하루 평균 식이섬유 추정 섭취량은 조사자에 따라 다소 차이가 있으나 20g 미만으로 잠정적 권장량에 미달하는 것으로 알려져 있다. 이와 같은 식이섬유 섭취량의 저하로 인해 과체중, 성인병 등의 발병률이 증가하여 국민의 건강에 심각한 위협이 되고 있다.However, the intake of dietary fiber is decreasing due to the introduction of westernized eating habits and excessive intake of nutrients, and the estimated daily average dietary fiber intake per person varies somewhat depending on the investigator, but it is known that it is less than 20 g, which is less than the tentative recommendation. Due to such a decrease in dietary fiber intake, the incidence of overweight and adult diseases increases, posing a serious threat to public health.
한편, 보리, 율무, 콩, 조, 기장, 수수, 옥수수 등과 같은 잡곡은 쌀에 비해 열등한 작물로서 여겨져 왔으나, 쌀에 부족한 영양성분을 보충하는 역할을 할 뿐만 아니라, 성인병 예방에 필요한 식이섬유, 비타민, 미네랄 등이 쌀에 비해 약 2 내지 3배 정도 풍부하여 기능성 소재로써 가치가 높은 것으로 알려지면서, 최근 쌀소비 감소와 재고미 증가에 따른 문제점을 해결하고 농가소득을 증진시키기 위한 소득 작물로 주목받고 있다.On the other hand, mixed grains such as barley, barley, soybean, millet, millet, sorghum, and corn have been regarded as inferior crops compared to rice, but they not only supplement the nutrients lacking in rice, but also provide dietary fiber and vitamins necessary for preventing adult diseases. , minerals, etc. are known to be high as functional materials because they are about 2 to 3 times richer than rice. have.
또한, 잡곡은 영양 공급적인 측면뿐만 아니라, 생체적 리듬과 신진대사 조절 또는 질병예방 등을 향상시키는 건강기능으로 초점이 전환되고 있으며, 이에 따라 기능적 측면의 식품의 소재로써 잡곡의 가치가 증가하고 있다.In addition, the focus of mixed grains is shifting not only to the nutritional supply, but also to the health functions that improve biological rhythm and metabolism control or disease prevention. .
하지만, 종래에는 대부분 잡곡을 밥에 섞어 먹는 혼반용으로 사용하고 있으며, 가공제품으로의 이용은 가공적성과 기호도 문제로 인하여 극히 제한적으로 이용되고 있는 실정으로, 식이섬유가 다량 함유된 귀리와 수수 등의 잡곡을 이용하여 빵이나 과자처럼 모든 사람들이 즐겨 먹을 수 있는 식품에 적용한다면 식이섬유가 풍부한 식품을 효율적으로 섭취할 수 있을 것으로 판단된다.However, conventionally, most of the grains are mixed with rice, and their use as processed products is extremely limited due to processing aptitude and preference problems. Oats and sorghum containing a large amount of dietary fiber It is judged that food rich in dietary fiber can be consumed efficiently if it is applied to foods that everyone can enjoy, such as bread and sweets, using the grains of
따라서, 식이섬유가 풍부한 잡곡섭취를 촉진하기 위해서는 소비자의 다양한 기호에 맞는 모양과 이화학적 특성을 갖춘 식품의 가공기술이 필요한 실정이다.Therefore, in order to promote the intake of multi-grains rich in dietary fiber, there is a need for a food processing technology having a shape and physicochemical characteristics suitable for various tastes of consumers.
본 발명의 발명자들은 난용성 곡물 소재가 장내 유익 미생물에 증식에 미치는 영향에 대한 상관관계에 대해 연구하던 중, 난용성 곡물을 팽화 처리한 다음 섭취하면 곡물 입자가 장내 유익 미생물이 서식하기 위한 공간 및 환경을 제공하고, 장내 식이섬유의 이용률이 증가되어 장내 유익 미생물 생육 증진효과를 발휘함을 확인하였고, 이와 더불어 식이섬유를 효과적으로 섭취할 수 있는 방법을 확립함으로써 본 발명을 완성하였다. While the inventors of the present invention were studying the correlation of the effect of poorly soluble grain material on the growth of beneficial microorganisms in the intestine, when the poorly soluble grain is expanded and then ingested, the grain particles form a space for beneficial intestinal microorganisms to inhabit and The present invention was completed by providing an environment, and by establishing a method for effectively ingesting dietary fiber, confirming that it exerts an effect of promoting beneficial intestinal microbial growth by increasing the utilization rate of dietary fiber in the intestine.
따라서, 본 발명은 프로바이오틱스(probiotics) 및 프리바이틱스(prebiotics)의 유용한 효과를 동시에 나타낼 수 있는 팽화 곡물과자의 제조방법에 대한 기술 내용을 제공하고자 하는 것이다.Accordingly, an object of the present invention is to provide technical details on a method for manufacturing puffed grain confectionery that can simultaneously exhibit the useful effects of probiotics and prebiotics.
상기한 바와 같은 기술적 과제를 달성하기 위해서 본 발명은, (a) 곡물을 분쇄하여 곡물 입자를 제조하는 단계; (b) 곡물 입자를 건조하는 단계; (c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계; (d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및 (e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는 팽화 곡물과자의 제조방법을 제공한다. In order to achieve the technical problem as described above, the present invention comprises the steps of: (a) pulverizing grains to produce grain particles; (b) drying the grain particles; (c) swelling the dry grain particles to produce expanded grain particles; (d) pulverizing the expanded grain particles to prepare a grain powder; And (e) provides a method for manufacturing puffed grain confectionery comprising the step of manufacturing a confectionery by molding the grain powder.
또한, 본 발명은 상기에 기재된 방법으로 제조된 팽화 곡물과자를 제공한다.In addition, the present invention provides a puffed grain snack prepared by the method described above.
본 발명에 따른 팽화 곡물과자는 식이섬유 및 유효성분의 체내 흡수율이 현저히 증가되어 장내 유익 미생물의 생육을 효과적으로 증진시킬 수 있을 뿐만 아니라, 기호성 및 유효성분의 함량이 증진된 팽화 곡물과자의 제조방법을 확립하였으며, 본 발명의 제조방법으로 제조한 팽화 곡물과자는 비피도 박테리아 등과 같은 장내 유익 미생물인 프로바이오틱스의 생육을 증진시키는 프리바이오틱스 물질로서, 이를 섭취하게 되면 병원성균에 의한 장 감염을 예방하고, 장내 균총의 균형을 유지할 수 있을 뿐만 아니라, 발암억제, 콜레스테롤 감소, 면역증강 등의 우수한 효능을 가지므로 유용하게 이용할 수 있다.The puffed grain snack according to the present invention can effectively promote the growth of beneficial intestinal microorganisms by significantly increasing the absorption rate of dietary fiber and active ingredients in the body, as well as improving palatability and content of active ingredients. It has been established, and the puffed grain snack produced by the manufacturing method of the present invention is a prebiotic material that promotes the growth of probiotics, which are beneficial intestinal microorganisms such as bifidobacteria, and prevents intestinal infection caused by pathogenic bacteria when ingested, Not only can it maintain the balance of the intestinal flora, but it can also be usefully used because it has excellent effects such as cancer suppression, cholesterol reduction, and immunity enhancement.
도 1은 장내 프로바이오틱 증진 난용성 곡물식이섬유 팽화 곡물과자의 제조공정을 나타낸 사진이다.
도 2는 곡물 추출물에서 배양한 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)의 활성이 증진됨을 나타낸 그래프이다.
도 3은 동결건조 곡물 열수추출물에서 배양한 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)의 활성이 증진됨을 나타낸 그래프이다.
도 4는 혼합곡물분말과 장내유익균 혼합물의 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다.
도 5는 3D 이미지로 구현한 혼합곡물과 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다.
도 6은 찰기장, 현미 및 귀리 각각의 분말 및 일정비율로 혼합된 곡물분말과 Lactobacillus acidophilus의 부착능을 나타낸 CLSM 이미지이다. 1 is a photograph showing the manufacturing process of the intestinal probiotic-promoting poorly soluble grain dietary fiber expanded grain confectionery.
Figure 2 shows four beneficial intestinal microorganisms ( Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius ) cultured in grain extracts. subsp . thermophilus It is a graph showing that the activity of KCTC 3658, Bifidobacterium bifidum KCTC 3357) is enhanced.
Figure 3 shows four beneficial intestinal microorganisms ( Lactobacillus acidophillus ) cultured in freeze-dried grain hot water extract. KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium It is a graph showing that the activity of bifidum KCTC 3357) is enhanced.
4 is a CLSM image showing the adhesion ability of Lactobacillus acidophilus of a mixture of mixed grain powder and beneficial intestinal bacteria.
5 is a CLSM image showing the adhesion ability of the mixed grain and Lactobacillus acidophilus realized as a 3D image.
6 is a CLSM image showing the adhesion ability of Lactobacillus acidophilus with each powder of sticky rice, brown rice and oats, and grain powder mixed at a certain ratio.
이하, 본 발명을 상세히 설명하도록 한다.Hereinafter, the present invention will be described in detail.
본 발명은 (a) 곡물을 분쇄하여 곡물 입자를 제조하는 단계; (b) 곡물 입자를 건조하는 단계; (c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계; (d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및 (e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는 팽화 곡물과자의 제조방법을 제공한다(도 1). The present invention comprises the steps of: (a) grinding grain to produce grain particles; (b) drying the grain particles; (c) swelling the dry grain particles to produce expanded grain particles; (d) pulverizing the expanded grain particles to prepare a grain powder; And (e) provides a method for manufacturing puffed grain confectionery comprising the step of manufacturing a confectionery by molding the grain powder (FIG. 1).
상기 단계 (a)의 곡물은 보리, 수수, 귀리, 율무, 기장, 식용피, 좁쌀, 찹쌀, 현미, 백미로 이루어진 군으로부터 선택되는 1종 이상을 포함하는 곡물을 분쇄하여 곡물 입자를 제조하는 단계이다. The grain of step (a) is barley, sorghum, oats, barley radish, millet, edible skin, millet, glutinous rice, brown rice, milling grains comprising at least one selected from the group consisting of white rice to prepare grain particles to be.
상기 단계 (a)의 곡물은 전체 팽화 곡물과자 총중량 대비 50 내지 55 중량%를 첨가하는 것이 바람직하나 이에 한정하지 않는다.The grain of step (a) is preferably added in an amount of 50 to 55% by weight based on the total weight of the total puffed grain confectionery, but is not limited thereto.
상기 곡물은 식이섬유의 포함함량이 높은 곡물을 선정하여 사용할 수 있으며, 보리, 수수, 귀리, 율무, 기장, 식용피, 좁쌀, 찹쌀, 현미 또는 백미 등과 같이 잡곡 및 쌀의 혼합물을 사용할 수 있으나, 바람직하게는, 조섬유의 포함함량이 7% 이상으로 높은 수수, 귀리, 율무, 식용피, 좁쌀, 기장, 찹쌀 또는 이들의 혼합물을 사용하여 장내 유익 미생물의 영양분이되는 프로바이오틱스 물질인 식이섬유를 다량 공급할 수 있다.The grains can be used by selecting grains with a high content of dietary fiber, and a mixture of grains and rice such as barley, sorghum, oats, barley, millet, edible skin, millet, glutinous rice, brown rice or white rice can be used, Preferably, a large amount of dietary fiber, which is a probiotic substance that serves as a nutrient for beneficial intestinal microorganisms, can be supplied by using sorghum, oats, barley radish, edible skin, millet, millet, glutinous rice, or a mixture thereof with a crude fiber content of 7% or more. can
상기 잡곡은 쌀밥에 부족한 영양성분 보충 역할 뿐만 아니라 성인병 예방에 필요한 식이섬유, 비타민, 미네랄 등이 쌀에 비해 대략 2 내지 3배 정도 풍부하여 기능성 소재로써 가치가 높다.The multi-grain has high value as a functional material because it not only supplements the nutritional components lacking in rice, but also contains about 2 to 3 times as much dietary fiber, vitamins, and minerals necessary for preventing adult diseases as compared to rice.
특히, 귀리는 7 내지 22%의 식이섬유와 3.8%의 베타-글루칸(β-glucan)이 함유되어 있어 지방 세포의 축적을 억제함으로써 체지방 형성을 감소시키고, HDL 콜레스테롤을 증가시켜 혈중 콜레스테롤의 수치를 감소시키며, 간에 콜레스테롤이 축적되는 것을 억제하며, 지방질의 소화기능을 저하하며, 지질 대사를 개선시켜 항비만 효과가 있다. 또한, 글로불린(globulin)은 다량 포함하고, 프롤라민의 함량은 낮으며, 라이신 등의 필수 아미노산하고, 팔미트산, 올레산, 리놀레익산 등의 지방산을 다량 포함하며, 비타민 B와 칼슘 등의 포함함량이 높아 우수한 곡물 소재로서, 생리활성 물질인 고분자 다당체가 다량 함유되어 있어 다양한 생리효능이 있고 식후 혈당상승억제 및 콜레스테롤 개선하는 효능을 가져 팽화 곡물과자 제조에 원료로 사용할 수 있다.In particular, oats contain 7 to 22% of dietary fiber and 3.8% of beta-glucan, which inhibits the accumulation of fat cells, thereby reducing body fat formation and increasing HDL cholesterol to lower blood cholesterol levels. It has an anti-obesity effect by reducing cholesterol accumulation in the liver, lowering the digestive function of lipids, and improving lipid metabolism. In addition, it contains a large amount of globulin, the content of prolamin is low, essential amino acids such as lysine, contains a large amount of fatty acids such as palmitic acid, oleic acid, linoleic acid, and contains vitamin B and calcium As an excellent grain material due to its high content, it contains a large amount of high molecular weight polysaccharides, which are physiologically active substances, and has various physiological effects.
상기 수수는 6 내지 8%의 식이섬유를 포함하며, 플라보노이드를 다량 포함하고, 탄닌, 페놀산, 안토시아닌, 피토스테롤, 폴리코사놀 등을 주요 성분으로 포함하여, 항암 효과, 면역기능 강화, 콜레스테롤 저하시키는 역할을 가져 팽화 곡물과자 제조에 원료로 사용할 수 있다.The sorghum contains 6 to 8% of dietary fiber, contains a large amount of flavonoids, and contains tannins, phenolic acids, anthocyanins, phytosterols, policosanol, etc. It can be used as a raw material for the manufacture of puffed grain confectionery.
상기 백미, 현미 등과 같은 쌀은 재배지역에 따라 다소 차이는 있으나, 백미의 경우, 가식부 100g당 75.5 g의 당질, 6.8 g의 단백질, 1.3 g의 지질 및 0.4 g의 조섬유를 포함하며, 회분을 대략 0.6 mg/100 g의 농도로 함유하고, 인, 칼슘, 마그네슘, 나트륨, 철분 등이 함유되어 팽화 곡물과자 제조에 이용할 수 있다. 또한, 쌀의 주된 단백질은 글루텔린(glutelin)계 오리제닌(oryzenin)을 포함하며, 소량의 글루불린과 알부민 등의 양질의 단백질을 포함한다.The rice, such as white rice and brown rice, is slightly different depending on the cultivation area, but in the case of white rice, it contains 75.5 g of sugar, 6.8 g of protein, 1.3 g of lipid, and 0.4 g of crude fiber per 100 g of edible part, and the ash content is approximately It is contained in a concentration of 0.6 mg/100 g, and phosphorus, calcium, magnesium, sodium, iron, etc. are contained, so it can be used for manufacturing puffed grain confectionery. In addition, the main protein of rice includes glutelin-based oryzenin, and a small amount of high-quality proteins such as globulin and albumin.
상기 단계 (a)에서는 건조한 곡물 입자를 팽화시키는 단계로서, 곡물 입자는 조직이 치밀한 상태로 수축되어 있어, 섭취시 식이섬유와 유효성분의 효율적인 섭취가 곤란한 문제가 있다.In the step (a), as the step of swelling the dried grain particles, the grain particles are contracted in a dense state, so that it is difficult to efficiently ingest dietary fiber and active ingredients during ingestion.
이에 따라, 본 단계에서는 곡물 입자를 팽화시켜, 식이섬유와 유효성분의 생체 흡수율을 증가시킬 수 있을 뿐만 아니라, 곡물의 갖는 구수한 맛과 향을 더욱 증진시켜 기호성을 높이고, 각종 이취를 제거된 곡물 입자를 제조할 수 있다.Accordingly, in this step, by swelling the grain particles, it is possible to increase the bioabsorption rate of dietary fiber and active ingredients, as well as improve the palatability by further enhancing the savory taste and flavor of the grains, and grain particles from which various odors are removed. can be manufactured.
이를 위해, 상기 단계 (c)에서는 곡물 입자를 수증기 공급이 가능한 밀폐형 즉석 팽화장치에 공급하고, 수증기를 주입하면서 가열하여 곡물 입자의 조직 내부로 수증기가 확산되도록 하면서, 곡물 입자의 세포벽을 부분적으로 파쇄하고, 일정 비율로 조직이 부풀러 망상조직을 형성하도록 하여 곡물 입자의 팽화를 유도할 수 있다. To this end, in step (c), the grain particles are supplied to a closed type instantaneous expansion device capable of supplying water vapor, and heated while injecting water vapor so that the water vapor is diffused into the tissue of the grain particles, and the cell walls of the grain particles are partially crushed. And, it is possible to induce swelling of grain particles by inflating the tissue at a certain ratio to form a network.
상기와 같이 밀폐형 즉석 팽화장치를 이용하여 곡물 입자를 팽화시키는 팽화 공정은 220 내지 330℃의 온도에서 수행할 수 있으며, 상기 팽화공정의 온도가 230℃를 초과하는 경우, 곡물 입자에 포함된 유효 성분이 열 분해되어, 유효성분의 포함함량이 떨어지는 문제가 있고, 상기 팽화공정의 온도가 220℃ 미만인 경우, 상기 곡물 입자의 팽화효율이 떨어지고, 팽화시간이 증가하는 문제가 있으며, 압력을 조절하여 보다 원활히 곡물 입자를 팽화시킬 수 있다.As described above, the swelling process of expanding the grain particles using the sealed instant swelling device may be performed at a temperature of 220 to 330°C, and when the temperature of the swelling process exceeds 230°C, the active ingredient contained in the grain particles This thermal decomposition has a problem in that the content of the active ingredient is lowered, and when the temperature of the swelling process is less than 220 ° C, the swelling efficiency of the grain particles is lowered, there is a problem that the swelling time increases, and the pressure is more Grain particles can be expanded smoothly.
또는, 상기 곡물 입자를 스크류가 구비된 압출장치에 장입하고 압출하는 방법으로 곡물 입자를 팽화시킬 수 있다. 이와 같은 압출(extrusion) 공정은, 곡물 입자의 가공성을 개선할 수 있는 방법으로, 곡물에 압출공정을 적용하게 되면 곡물 입자에 포함된 전분, 단백질, 지질 성분들이 고온 상태에서 변형되어 점탄성을 갖게 되고, 수증기나 가스 등에 의해 부피가 증가하며 구조가 파괴되어 조직 재형성을 꾀할 수 있을 뿐만 아니라, 기계적 에너지에 의해 비교적 짧은 시간내에 팽화 곡물 입자를 제조할 수 있는 장점을 갖는다.Alternatively, the grain particles may be expanded by charging and extruding the grain particles into an extrusion device equipped with a screw. This extrusion process is a method that can improve the processability of grain particles. When the extrusion process is applied to grains, starch, protein, and lipid components contained in the grain particles are deformed at a high temperature to have viscoelasticity. , water vapor or gas increases the volume and the structure is destroyed, so that tissue reformation can be attempted, and the expanded grain particles can be manufactured in a relatively short time by mechanical energy.
이를 위해, 상기 압출공정으로 곡물 입자를 팽화시키기 위해서는, 건조한 곡물 입자를 특정 입자 크기를 갖도록 분쇄하여 팽화 공정을 수행할 수 있으며, 바람직하게는 30 내지 60 메쉬(mesh)의 입자크기를 갖는 곡물 분말을 제조한 후, 압출장치에 장입하여 압출공정을 수행하여 팽화 효율을 극대화시킬 수 있다.To this end, in order to expand the grain particles by the extrusion process, the dry grain particles may be pulverized to have a specific particle size to perform a swelling process, and preferably, a grain powder having a particle size of 30 to 60 mesh (mesh). After manufacturing, it is charged in an extrusion device to perform the extrusion process to maximize the swelling efficiency.
상기와 같이 압출장치를 이용할 경우, 상기 팽화 공정은 바렐부의 온도를 90 내지 130℃로 조절하고, 투입속도를 고려하여 수분함량을 조절하는 방법으로 곡물 입자를 압출시킴에 따라, 곡물 입자에 팽화를 유도할 수 있으며, 이에 의해 식이섬유 및 유효성분의 감소가 극소화된 우수한 품질의 팽화 곡물 입자를 제조할 수 있다.In the case of using the extrusion apparatus as described above, in the expansion process, the temperature of the barrel part is adjusted to 90 to 130° C., and the grain particles are extruded by controlling the moisture content in consideration of the input speed, thereby increasing the swelling of the grain particles. It can be induced, and thereby, it is possible to manufacture expanded grain particles of excellent quality with minimal reduction in dietary fiber and active ingredients.
상기와 같이 팽화가 유도된 곡물 입자는 곡물 입자의 세포벽이 부분적으로 파쇄된 상태로 곡물 입자에 포함된 식이섬유 및 유효성분의 생체 흡수율을 향상시킬 수 있을 뿐만 아니라, 팽화에 의해 곡물 자체가 갖는 구수한 맛과 향이 더욱 강해짐에 따라 기호성이 현저히 향상되게 된다.The grain particles induced by swelling as described above can improve the bioabsorption rate of dietary fiber and active ingredients contained in grain grains with the cell walls of grain grains partially crushed, as well as improve the taste of grain itself by swelling. As the taste and aroma become stronger, the palatability is significantly improved.
특히, 상기와 같이 팽화처리한 팽화 곡물 입자는 1 내지 3의 팽화도를 가지며, 곡물 입자의 세포벽이 부분적으로 파쇄되어 수분흡착지수가 현저히 향상될 뿐만 아니라, 장내 유익균의 영양분인 식이섬유가 장내에서 다량 흡수되고, 장내 유익 미생물의 생육공간 및 환경을 조성하여 섭취자의 건강 및 면역력 향상에 기여할 수 있다.In particular, the expanded grain particles treated as described above have a swelling degree of 1 to 3, and the cell wall of the grain particles is partially crushed to significantly improve the water absorption index, and dietary fiber, which is a nutrient for beneficial intestinal bacteria, is stored in the intestine. It is absorbed in a large amount and can contribute to the health and immunity improvement of the ingester by creating a growth space and environment for beneficial microorganisms in the intestine.
상기 단계 (d)에서는 팽화시킨 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계로서, 본 단계에서는 상기 곡물 분말을 특정한 입자 크기를 갖는 분말 형태로 분쇄하여 후술할 단계에서 각종 첨가물과 혼합하여 다양한 형상 및 모양을 갖도록 성형됨으로써, 기호성이 높은 팽화 곡물과자를 제조할 수 있게 된다.In step (d), the expanded grain particles are pulverized to prepare grain powder. In this step, the grain powder is pulverized into a powder having a specific particle size and mixed with various additives in a step to be described later to obtain various shapes and By molding to have a shape, it is possible to manufacture puffed grain confectionery with high palatability.
상기 단계 (e)에서는, 상기 팽화 곡물 분말을 성형하여 팽화 곡물과자를 제조하는 단계로서, 본 단계에서는 상기 팽화 곡물 분말을 포함하는 곡물 조성물을 제조하고, 제조한 곡물 조성물을 성형하여 다양한 크기 및 형상을 갖는 팽화 곡물과자를 제조할 수 있으며, 통상적인 분말을 이용한 과자의 제조방법이라면 제한받지 않고 사용할 수 있다.In the step (e), as a step of manufacturing the expanded grain confectionery by molding the expanded grain powder, in this step, a grain composition comprising the expanded grain powder is prepared, and the prepared grain composition is molded to form various sizes and shapes. It is possible to manufacture puffed grain confectionery having
상기 곡물 조성물은 팽화 곡물 분말 이외에도 다양한 맛과 향을 구현할 수 있도록, 다양한 첨가물을 첨가하여 제조할 수 있으며, 특히, 상기 첨가물로 시즈닝, 팥, 홍삼, 설탕, 물엿, 소르비톨 또는 정제염 등의 조미원료를 첨가하여 기호성이 더욱 향상된 팽화 곡물과자를 제조하도록 구성할 수 있다. 상기 시즈닝은 백설탕, 포도당, 유당, 탈지분유, 향신료, 코코아 분말, 메이플향, 천일염, 건생강분말 및 수크랄로스를 배합하여 추러스 맛을 낸것을 이용할 수 있다.The grain composition can be prepared by adding various additives to implement various tastes and flavors in addition to the puffed grain powder. By adding it, it can be configured to produce puffed grain cookies with improved palatability. For the seasoning, white sugar, glucose, lactose, powdered skim milk, spices, cocoa powder, maple flavor, sea salt, dry ginger powder and sucralose may be mixed to give a churros flavor.
상기한 바와 같은 본 발명에 따른 팽화 곡물과자의 제조방법에 따르면, 곡물을 팽화시켜 팽화 곡물을 제조하고, 이를 곡물과자의 제조에 이용함으로써, 식이섬유 및 유효성분의 체내 흡수율이 현저히 증가되어 장내 유익 미생물의 생육을 효과적으로 증진시킬 수 있을 뿐만 아니라, 기호성 및 유효성분의 함량이 증진된 팽화 곡물과자를 제조할 수 있다.According to the method for manufacturing puffed grain confectionery according to the present invention as described above, by expanding the grain to produce puffed grain, and using it for the production of grain confectionery, the absorption rate of dietary fiber and active ingredient in the body is remarkably increased to benefit the intestines It is possible not only to effectively promote the growth of microorganisms, but also to prepare puffed grain confectionery having improved palatability and content of active ingredients.
또한, 쌀 및 잡곡의 소비를 촉진시키기 위한 방편으로 활용되어 농가소득 증진에 기여할 수 있다.In addition, it can be used as a way to promote the consumption of rice and coarse grains, contributing to the increase of farm household income.
상기 방법으로 제조한 팽화 곡물과자는 비피도 박테리아 등과 같은 장내 유익 미생물인 프로바이오틱스의 생육을 증진시키는 프리바이오틱스 물질로서, 이를 섭취하게 되면 병원성균에 의한 장 감염을 예방하고, 장내 균총의 균형을 유지할 수 있을 뿐만 아니라, 발암억제, 콜레스테롤 감소, 면역증강 등의 우수한 효능을 갖는다.The puffed grain snack prepared by the above method is a prebiotic material that promotes the growth of probiotics, which are beneficial intestinal microorganisms such as bifidobacteria, and when ingested, prevents intestinal infections caused by pathogenic bacteria and maintains the balance of intestinal flora In addition to being able to, it has excellent effects such as cancer suppression, cholesterol reduction, and immune enhancement.
따라서, 본 발명은 상기에 기재된 방법으로 제조된 팽화 곡물과자를 제공한다.Accordingly, the present invention provides a puffed grain snack prepared by the method described above.
상기 팽화 곡물과자는 팽화 곡물을 포함하여 장내에서 곡물에 포함된 식이섬유의 흡수율을 증진시키고, 식이섬유를 장내 유익 미생물이 용이하게 섭취할 수 있어 장내 유익 미생물의 생육을 증진시키는 효과를 갖는다.The puffed grain confectionery has the effect of enhancing the absorption rate of dietary fiber contained in grains in the intestine, including puffed grains, and promoting the growth of beneficial intestinal microbes because dietary fiber can be easily ingested by beneficial intestinal microbes.
상기 장내 유익 미생물은 유산균 등과 같은 장내 유익균이라면, 제한되지 않으나, 특히, 락토바실러스 에시도필러스(Lactobacillus acidophillus), 락토바실러스 델브룩키 스페이시즈 락티스(Lactobacillus delbrueckii subsp. lactis), 스테렙토코커스 살리바리어스 스페이시즈 써모필러스(Streptococcus salivarius subsp. thermophilus), 비피도박테리움 비피덤(Bifidobacterium bifidum) 등과 같은 장내 유익 미생물의 생육을 효과적으로 증진시키는 효과를 갖는다.The beneficial microorganisms in the intestine are not limited as long as they are beneficial bacteria such as lactic acid bacteria, but in particular, Lactobacillus acidophillus , Lactobacillus delbrueckii subsp. lactis ), Stereptococcus sali Streptococcus salivarius ( Streptococcus salivarius ) subsp . thermophilus ), Bifidobacterium bifidum ) has the effect of effectively promoting the growth of beneficial microorganisms in the intestine, such as.
이하, 본 발명을 실시예를 들어 더욱 상세히 설명하도록 한다.Hereinafter, the present invention will be described in more detail by way of examples.
제시된 실시예는 본 발명의 구체적인 예시일 뿐이며, 본 발명의 범위를 제한하기 위한 것은 아니다.The presented examples are only specific examples of the present invention, and are not intended to limit the scope of the present invention.
<< 실시예Example 1> 팽화 곡물과자의 제조 방법 1> Manufacturing method of puffed grain cookies
난곡성 곡물 유래 섬유소를 프리바이오틱스(Prebiotics) 소재로 활용한 팽화 곡물과자를 제조하였다. A puffed grain snack was prepared using non-grain-derived fiber as a prebiotics material.
구체적으로, 곡물(귀리, 현미, 백미 및 옥수수)을 하기 표 1에 기재된 배합비로 볼밀링 장치에 넣어 분쇄하여 30 내지 60 메쉬(mesh)의 평균입자 크기를 갖는 곡물 분말을 제조하였다. 후술할 압출공정에서 곡물 분말의 투입속도를 고려하여 곡물 분말의 수분함량을 30%가 되도록 조절하였고, 4℃에서 24시간 동안 평형화시킨 후 사용하였다.Specifically, grains (oats, brown rice, white rice and corn) were put into a ball milling apparatus at the mixing ratios shown in Table 1 below and pulverized to prepare a grain powder having an average particle size of 30 to 60 mesh. In the extrusion process to be described later, the moisture content of the grain powder was adjusted to 30% in consideration of the input rate of the grain powder, and it was used after equilibration at 4°C for 24 hours.
제조한 곡물 분말을 압출하여 곡물 분말을 팽화시켰으며, 팽화는 곡물 분말을 쌍축압출성형기[인천기계공업(주)]에 장입하여 압출공정을 수행하여 곡물과자를 제조하였다. 쌍축압출성형기는 동방향 스크류가 구비되고, 내부직경이 31.2 mm인 바렐 4개가 구비되어 17.3 미들-샤프트(middle-shaft)를 유지할 수 있는 것을 사용하였고, 150 rpm으로 회전속도를 고정하고, 바렐Ⅱ의 온도는 90℃, 바렐Ⅲ의 온도는 120℃, 바렐 Ⅳ의 온도는 130℃로 설정하여 압출성형공정을 수행하였다.The prepared grain powder was extruded to expand the grain powder, and the swelling was performed by charging the grain powder into a twin-screw extrusion molding machine [Incheon Machinery Industry Co., Ltd.] and performing an extrusion process to manufacture grain cookies. The twin-screw extrusion molding machine was equipped with a co-direction screw and 4 barrels with an inner diameter of 31.2 mm were used to maintain 17.3 middle-shaft, the rotation speed was fixed at 150 rpm, and the barrel II The extrusion molding process was performed by setting the temperature of 90 °C, the temperature of Barrel III to 120 °C, and the temperature of Barrel IV to 130 °C.
<< 실시예Example 2> 최적 2> Optimal 프리바이오틱스prebiotics (( prebioticsprebiotics ) 소재 선정) material selection
<2-1> 곡물 <2-1> Grain 열수hot water 추출물을 이용한 장내미생물 증진 활성 분석 Intestinal microorganism promotion activity analysis using extracts
상기 실시예 1에서 제조한 팽화 곡물과자를 위한 최적 프리바이오틱스 소재를 선정하기 위해서, 난용성 유기농 소재인 잡곡과 쌀을 포함하는 곡물이 장내 유익 미생물 및 유산균의 증식에 미치는 영향을 분석하였다.In order to select the optimal prebiotic material for the puffed grain confectionery prepared in Example 1, the effect of grains including mixed grains and rice, which are poorly soluble organic materials, on the growth of beneficial intestinal microorganisms and lactic acid bacteria was analyzed.
구체적으로, 곡물(귀리, 백미, 현미 및 옥수수)을 난용성 유기농 소재로 선정하여 사용하였으며, 상기 곡물을 분쇄기(DA-10000G, Hanil, Seoul, Korea)를 사용하여 100 mesh size 이하로 분쇄한 분말 각각을 증류수에 첨가하여 10분간 혼합한 후, 원심분리(4℃, 13,000 rpm, 10분)하고, 0.2 ㎛ 공경 여과지를 이용해 여과하여 식이섬유 추출액을 제조하였다. 제조한 식이섬유 추출액이 장내 유익 미생물 및 유산균은 미쯔오카 방법(Mitsuoka method)에 의해 인체에서 분리한 장내 유익 미생물 및 발효식품에서 분리한 유산균에서 탐색한 균주로, 장내 유익 미생물 및 유산균을 각각 RCM 배지 및 MRS 배지에 접종한 후, 배양하고, 배양 후 50%(v/v) 글리세롤을 배양액에 첨가하여 70℃ 이하의 초저온냉장고(deep freezer)에 보관하였다. 상기 장내 유익 미생물 및 유산균을 사용 전 3회 이상 계대 배양 후 활성화하여 사용하였다.Specifically, grains (oats, white rice, brown rice and corn) were selected and used as poorly soluble organic materials, and the grains were pulverized to 100 mesh size or less using a grinder (DA-10000G, Hanil, Seoul, Korea). Each was added to distilled water and mixed for 10 minutes, centrifuged (4 ℃, 13,000 rpm, 10 minutes), and filtered using 0.2 ㎛ pore size filter paper to prepare a dietary fiber extract. Intestinal beneficial microorganisms and lactic acid bacteria in the prepared dietary fiber extract are strains searched from beneficial intestinal microorganisms and lactic acid bacteria isolated from fermented foods isolated from the human body by the Mitsuoka method. And after inoculation in MRS medium, cultured, 50% (v/v) glycerol was added to the culture solution after culture, and stored in a deep freezer at 70° C. or less. The beneficial intestinal microorganisms and lactic acid bacteria were used after being subcultured at least 3 times before use and activated.
상기 장내 유익 미생물 및 유산균 2.5 mL을 4.25 mL의 RCM 배지 및 MRS 배지에 각각 접종한 후, 상기 곡물에서 추출한 식이섬유 추출액 5mL씩을 가해 37℃에서 72시간 동안 진탕배양한 후, 흡광도 측정장치(spectrophotometer)로 660nm 파장의 빛에서 배양시간(0, 4h, 8h, 12h, 16h, 20h, 24h, 48h, 72h)에 따른 흡광도 및 생균수 변화를 측정하는 방법으로 곡물이 증식에 미치는 영향을 분석하였다. 이때, 대조군으로는 곡물에서 추출한 식이섬유 추출액 대신 5mL의 DMSO(30%)를 첨가하여 동일하게 측정하였으며, 흡광도 및 생균수를 측정하였다.After inoculating 2.5 mL of the beneficial intestinal microorganisms and lactic acid bacteria into 4.25 mL of RCM medium and MRS medium, respectively, 5 mL of the dietary fiber extract extracted from the grain was added and cultured with shaking at 37° C. for 72 hours, then absorbance measuring device (spectrophotometer) The effect on the growth of grains was analyzed by measuring the change in absorbance and the number of viable cells according to incubation time (0, 4h, 8h, 12h, 16h, 20h, 24h, 48h, 72h) in light of 660 nm wavelength. At this time, as a control, 5 mL of DMSO (30%) was added instead of the dietary fiber extract extracted from grains and the same was measured, and the absorbance and the number of viable cells were measured.
그 결과, 대조군에 비해 각각의 곡물 분말의 추출물을 첨가한 배지에서는 흡광도가 현저히 증가하는 것을 확인할 수 있었고, 생균수가 30% 이상 증가하는 것을 확인하였다.As a result, it was confirmed that the absorbance was significantly increased in the medium to which the extract of each grain powder was added, compared to the control group, and the number of viable cells increased by 30% or more.
(CFU/mL)Control 1 )
(CFU/mL)
(CFU/mL)Grain powder 2 )
(CFU/mL)
subsp. lactis Lactobacillus delbrueckii
subsp. lactis
subsp. thermophilus Streptococcus salivarius
subsp . thermophilus
1) 대조군: 50%(w/v) MRS broth + 50% d 1) Control: 50% (w/v) MRS broth + 50% d
2)곡물분말 : 50%(w/v) MRS broth + 50% 곡물 분말의 추출물 2) Grain powder: 50% (w/v) MRS broth + 50% grain powder extract
3)성장률: 곡물분말의 생존 세포 수/대조군의 생존 세포 수 3) Growth rate: number of viable cells in grain powder/number of viable cells in control group
<2-2> 곡물 <2-2> Grain 열수hot water 추출물을 이용한 장내미생물 증진 활성 분석 Intestinal microorganism promotion activity analysis using extracts
곡물의 열수 추출물이 장내 유익 미생물의 생육에 미치는 영향을 분석하기 위해서, 곡물(귀리, 찰보리, 율무, 찰기장, 찰수수 및 현미)을 증류수에 투입하고, 55℃로 24시간 동안 가열하여 곡물 열수추출물을 제조하였으며, 제조한 열수추출물을 공경 5㎛의 여과지로 여과하였다. 한국생명공학연구원 생물자원센터에서 분양받아 활성화시킨 장내 유익 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)을 37℃에서 24시간 전배양 후, 3회 계대 배양하여 균주를 활성시킨 다음 계대배양 된 균주 5%(105 CFU/mL 농도)를 각각의 50% 곡물 열수 추출물에 접종하고50% MRS broth 혼합액에 분주하고, 48시간 배양시킨 후 시간 별(4h,8h,12h,18h,24h,36h,48h)로 흡광광도계를 이용하여 흡광도를 측정하여 그로스커브를 작성하여 배양시간별 흡광도 변화를 측정하였다In order to analyze the effect of the hot water extract of grains on the growth of beneficial microorganisms in the intestine, grains (oats, barley, barley, sticky rice, millet, and brown rice) were added to distilled water and heated at 55 ° C for 24 hours to prepare the grain hot water extracts. prepared, and the prepared hot water extract was filtered with a filter paper having a pore diameter of 5 μm. 4 types of beneficial intestinal microorganisms ( Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp . thermophilus After pre-culturing KCTC 3658, Bifidobacterium bifidum KCTC 3357) at 37°C for 24 hours, the strain was activated by subculture three times, and then 5% of the subcultured strain (10 5 CFU/mL concentration) was added to each 50% grain hot water extract. After inoculation with 50% MRS broth mixed solution, incubated for 48 hours, absorbance was measured using an absorbance meter for each hour (4h, 8h, 12h, 18h, 24h, 36h, 48h), and a growth curve was prepared and cultured. The change in absorbance over time was measured.
그 결과, 상기 곡물 추출물에서 배양한 장내 유익 미생물 4종 모두 대조군에 비해 유의적인 생육 증진 효과가 있는 것을 확인하였다(도 2).As a result, it was confirmed that all four types of beneficial intestinal microorganisms cultured in the grain extract had a significant growth promoting effect compared to the control group (FIG. 2).
<2-3> 동결건조 곡물 <2-3> Freeze-dried grains 열수추출물을hot water extract 이용한 장내미생물 증진 활성 분석 Intestinal microorganism promotion activity analysis using
동결건조한 곡물의 열수추출물이 장내 유익 미생물에 생육에 미치는 영향을 분석하였다.The effect of the hot water extract of freeze-dried grains on the growth of beneficial intestinal microorganisms was analyzed.
구체적으로, 동결건조한 곡물(귀리, 찰보리, 율무, 찰기장, 찰수수 및 현미)의 열수추출물에서 생육하는 장내 유익 미생물의 성장곡선(growth curve)을 도출하였으며, 동결건조한 곡물 각각의 열수추출물을 제조하고, 제조한 열수 추출물을 감압농축기로 농축한 후, 동결건조기로 건조하여 50 mg/mL의 농도를 갖는 동결건조 곡물의 열수추출물을 제조하였다. 제조한 열수추출물을 배양 배지로 이용하여 미생물 4종(Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp. thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357)을 접종하여 배양하였으며, 각각의 장내 유익 미생물이 증식하는 성장 곡선을 수득하였다.Specifically, the growth curve of beneficial intestinal microorganisms growing in the hot water extracts of freeze-dried grains (oats, barley, barley radish, sticky rice, millet and brown rice) was derived, and the hot water extracts of each of the freeze-dried grains were prepared, The prepared hot water extract was concentrated with a vacuum concentrator, and then dried with a freeze dryer to prepare a hot water extract of freeze-dried grains having a concentration of 50 mg/mL. Using the prepared hot water extract as a culture medium, 4 types of microorganisms ( Lactobacillus acidophillus KCTC 3164, Streptococcus salivarius subsp . thermophilus KCTC 3658, Bifidobacterium bifidum KCTC 3357) was inoculated and cultured, and a growth curve was obtained in which beneficial intestinal microorganisms proliferate.
그 결과, 락토바실러스 에시도필러스(Lactobacillus acidophillus KCTC 3164)는 찰보리, 율무, 귀리 등의 곡물 열수추출물에서 가장 높은 생육 증진효과를 보였으며, 현미를 제외한 다른 곡물에서 모드 유의적인 생육 증진효과를 나타내었다. 락토바실러스 델브룩키 스페이시즈 락티스(Lactobacillus delbrueckii subsp. lactis KCTC 3636)는 율무에서 가장 높은 증진효과를 갖는 것으로 확인되었고, 귀리 등 기타 곡물에서 유의적인 생육 증진효과를 나타낸다는 사실을 확인할 수 있었다. 스테렙토코커스 살리바리어스 스페이시즈 써모필러스(Streptococcus salivarius subsp. thermophilus KCTC 3658)의 경우에는 찰보리와 찰수수에서 가장 높은 생육 증진효과를 나타내었고, 귀리 등 다른 곡물에서도 생육 증진 효과를 나타내었다. 비피도박테리움 비피덤(Bifidobacterium bifidum KCTC 3357)의 경우에는 찰보리, 귀리, 찰수수에서 높은 증진효과를 나타내었고, 다른 곡물 모두 유의한 장내유익균 생육증진 효과를 보이는 것을 확인하였다(도 3).As a result, Lactobacillus acidophillus KCTC 3164 showed the highest growth promoting effect in the hot-water extracts of grains such as barley, barley, and oats, and showed a significant growth promoting effect in all grains except brown rice. It was. Lactobacillus delbrueckii spaces lactis ( Lactobacillus delbrueckii subsp. lactis KCTC 3636) was confirmed to have the highest enhancing effect in barley radish, and it was confirmed that it showed a significant growth promoting effect in other grains such as oats. Streptococcus salivarius subsp . thermophilus In the case of KCTC 3658), brown barley and sorghum showed the highest growth-promoting effect, and other grains such as oats also exhibited a growth-promoting effect. Bifidobacterium Bifidobacterium bifidum KCTC 3357) showed a high enhancing effect in brown barley, oats, and sorghum, and it was confirmed that all other grains showed significant beneficial intestinal growth promoting effects (FIG. 3).
상기와 같은 결과를 통해서, 상기 곡물 소재를 활용하면, 식이섬유를 고농도로 함유하면서 장내유익균의 생육을 증진시킬 수 있는 팽화식품 소재를 제조할 수 있음을 확인할 수 있었으며, 이를 통해 프리바이오틱스 소재를 특정 지을 수 있었다.Through the above results, it was confirmed that if the grain material was used, it was possible to manufacture a puffed food material capable of enhancing the growth of beneficial intestinal bacteria while containing dietary fiber at a high concentration, and through this, the prebiotic material could be specific.
<< 실시예Example 3> 장내미생물 거동 모델 분석 3> Intestinal microbial behavior model analysis
<3-1> <3-1> 프리바이오틱스prebiotics 곡물분말과 grain powder and 장내유익균간의between beneficial intestinal bacteria 흡착도 분석 Adsorption analysis
곡물 분말에서 장내 유익 미생물이 나타내는 거동을 분석하기 위해서, 공초점 주사 레이져 현미경(Confocal laser Scanning Microscopy, CLSM)을 이용하여 프리바이오틱스(Prebiotics) 곡물소재의 영상 이미지를 수득하였으며, 곡물분말(귀리, 현미, 백미 및 옥수수)에 장내 유익 미생물인 락토바실러스 에시도필러스(Lactobacillus acidophillus KCTC 3164)을 접종하여 수수 분말에 대한 장내 유익 미생물의 부착능을 확인하였다.To analyze the behavior of beneficial intestinal microbes in grain powder, image images of prebiotics grain material were obtained using Confocal Laser Scanning Microscopy (CLSM), and grain powder (oat, Brown rice, white rice, and corn) were inoculated with Lactobacillus acidophillus KCTC 3164, a beneficial intestinal microorganism, to confirm the ability of the intestinal beneficial microorganism to adhere to the sorghum powder.
그 결과, 곡물 분말이 장내에서 장내 유익 미생물의 서식을 위한 공간 및 환경을 제공하여 장내 유익 미생물의 증식 및 생육에 도움을 줄 수 있을 것으로 판단되었다(도 4).As a result, it was determined that the grain powder could help the proliferation and growth of beneficial intestinal microorganisms by providing a space and environment for the inhabitation of beneficial intestinal microorganisms in the intestine (FIG. 4).
<3-2> 3D 이미지로 구현한 <3-2> Realized as 3D image 프리바이오틱스prebiotics 곡물분말과 grain powder and 장내유익균간의between beneficial intestinal bacteria 흡착도 분석 Adsorption analysis
곡물(귀리, 현미, 백미 및 옥수수)에서 장내 유익 미생물이 나타내는 거동을 분석하기 위해서, 장내 유익 미생물의 CLSM 3차원 이미지를 수득하였다. In order to analyze the behavior of beneficial gut microbes in grains (oats, brown rice, white rice and corn), CLSM three-dimensional images of gut beneficial microbes were obtained.
구체적으로, CLSM 3D 이미지 분석을 위한 최적 조건을 설정하고, 프리바이오틱스 곡물의 장내 유익 미생물 흡착도를 분석하였으며, 장내 유익 미생물을 24시간 동안 배양한 후, 4,000 rpm으로 5분간 원심분리하여, 상층액과 침전균체를 분리하였다. 분리한 균체를 PBS 완충용액(PBS buffer solution)으로 1회 세척한 후, 0.03% 형광이소티오시아네이트(fluorescein isothiocyanate, FITC)로 10분 동안 암실에서 염색하였으며, 염색한 후, PBS 완충용액으로 3회 세척한 후, CLSM으로 확인하였다.Specifically, the optimal conditions for CLSM 3D image analysis were set, and the intestinal beneficial microorganisms adsorption of prebiotic grains were analyzed, and the beneficial intestinal microorganisms were cultured for 24 hours, then centrifuged at 4,000 rpm for 5 minutes, and the upper layer was Liquid and precipitated cells were separated. The isolated cells were washed once with PBS buffer solution, and then stained with 0.03% fluorescein isothiocyanate (FITC) in the dark for 10 minutes. After washing twice, it was confirmed by CLSM.
그 결과, 곡물 분말이 장내에서 장내 유익 미생물의 서식을 위한 공간 및 환경을 제공하여 장내 유익 미생물의 증식 및 생육에 도움을 줄 수 있을 것으로 판단되었다(도 5).As a result, it was determined that the grain powder could help the proliferation and growth of beneficial intestinal microorganisms by providing a space and environment for the inhabitation of beneficial intestinal microorganisms in the intestine (FIG. 5).
<3-3> <3-3> 프리바이오틱스prebiotics 곡물분말과 장내 유익 미생물의 흡착도 분석 Analysis of adsorption of grain powder and beneficial intestinal microbes
프리바이오틱스 혼합곡물분말(귀리, 현미, 백미 및 옥수수)과 장내 유익 미생물의 흡착도 확인하기 위해서, 곡물분말(귀리, 현미, 백미 및 옥수수), 현미, 귀리 분말 및 찰기장과 장내 유익 미생물을 실험을 통해 정해진 적정 형광염료와 농도를 이용하여 염색 후 CLSM을 이용하였으며, 곡물 분말과 균주의 혼합시 곡물의 자가형광이 넓은 파장대에서 강한 강도를 나타냄으로 균주와 구분의 명확하지 않아 DIC 필터(differential interference contrast filter)를 사용하여 상을 관찰하였고, 균주는 0.03% FITC를 사용하여 염색 후 곡물분말과 혼합하여 관찰하였다. In order to confirm the adsorption of prebiotic mixed grain powder (oats, brown rice, white rice and corn) and beneficial intestinal microbes, we tested grain powder (oats, brown rice, white rice and corn), brown rice, oat powder, sticky rice and beneficial intestinal microbes. CLSM was used after dyeing using an appropriate fluorescent dye and concentration determined through the DIC filter (differential interference contrast filter) was used to observe the phase, and strains were observed by mixing with grain powder after staining using 0.03% FITC.
그 결과, 장내 유익 미생물이 귀리와 혼합곡물분말(귀리, 현미, 백미 및 옥수수)의 주위로 부착되어 장내 유익 미생물이 곡물의 표면에 부착되는 곡물의 부착능이 찰기장 부착능보다 뛰어난 것을 확인하였으며, 혼합곡물분말이 장내에서 장내 유익 미생물의 서식을 위한 공간과 환경을 제공하여 장내미생물의 증식에 도움을 주는 것을 확인하였다(도 6).As a result, it was confirmed that beneficial intestinal microorganisms were attached around oats and mixed grain powders (oats, brown rice, white rice, and corn), and the adhesion ability of the grains where beneficial intestinal microorganisms were attached to the surface of the grain was superior to that of sticky rice. It was confirmed that the grain powder helps the growth of intestinal microorganisms by providing a space and environment for the inhabitation of beneficial intestinal microorganisms in the intestine (FIG. 6).
<< 실시예Example 4> 난용성 4> poorly soluble 프로바이오틱스probiotics 팽화 곡물 과자의 특성 분석 Characterization of puffed grain confectionery
<4-1> 압출 성형한 잡곡의 특성 분석<4-1> Characteristics analysis of extruded grains
압출 성형하여 팽화시킨 잡곡의 팽화도를 측정하여 팽화 효율을 분석하였다. The swelling efficiency was analyzed by measuring the swelling degree of the coarse grains expanded by extrusion molding.
하기 표 3에 나타난 바와 같이, 압출 성형하여 제조한 곡물의 팽화도는 1.70 내지 2.23의 범위에 속하였으며, 율무가 2.23으로 가장 높고, 찰보리가 1.74으로 가장 낮은 팽화도 값을 나타내었다. As shown in Table 3 below, the swelling degree of the grain prepared by extrusion was in the range of 1.70 to 2.23, Adulmu had the highest value of 2.23, and barley had the lowest swelling value of 1.74.
<4-2> 압출성형 잡곡의 수분용해지수 및 수분 흡착지수 분석<4-2> Analysis of water dissolution index and water adsorption index of extruded grains
압출성형하여 제조한 곡물의 수분용해지수 및 수분 흡착지수를 분석하였다. The water dissolution index and water adsorption index of grains prepared by extrusion molding were analyzed.
하기 표 4에 나타낸 바와 같이, 압출성형하여 제조한 곡물의 수분용해지수 및 수분흡착지수는 팽화에 의해 현저히 증가하는 것으로 확인되었으며, 수분흡착지수는 보리가 가장 높은 것으로 나타났고, 특히, 현미는 대조군이 갖는 0.22 보다 팽화 후 3.37%로 현저히 증가하는 것으로 확인되었다. As shown in Table 4 below, it was confirmed that the water dissolution index and water adsorption index of the grains prepared by extrusion molding were significantly increased by swelling, and the water adsorption index was found to be the highest in barley, in particular, brown rice as a control group It was confirmed that it significantly increased to 3.37% after swelling rather than 0.22 having this.
experimental material
<< 실시예Example 5> 팽화 곡물과자의 관능평가 5> Sensory evaluation of puffed grain cookies
실시예 1에 따른 방법으로 제조하여 팽화 곡물과자의 관능평가는 식품을 전공하는 대학생 20명을 선정하여 수행하였다. Sensory evaluation of puffed grain cookies prepared by the method according to Example 1 was performed by selecting 20 college students majoring in food.
관능평가는 곡물과자의 맛, 색, 씹힘성, 전반적인 기호도에 대한 특성을 5점 척도법으로 평가하여 '매우 나쁘다'를 1점, '매우 좋다'를 5점으로 1 내지 5점의 범위에서 점수를 부여하여 선호도가 높을수록 높은 점수를 주도록 설정하여 수행하였다. 또한, 시판되고 있는 A 곡물과자를 대조군으로 하여 제조한 팽화 곡물과자와 비교하여 관능평가를 수행하였고, 그 결과를 하기 표 5에 나타내었다(단, 하기 표 5에서 1은 대단히 싫어한다, 2는 싫어한다, 3은 보통이다, 4는 좋아한다, 5는 매우 좋아한다를 나타냄).In the sensory evaluation, the characteristics of taste, color, chewiness, and overall preference of grain confectionery are evaluated on a 5-point scale, with 1 point for 'very bad' and 5 points for 'very good', giving a score in the range of 1 to 5. Therefore, the higher the preference, the higher the score was set and performed. In addition, the sensory evaluation was performed in comparison with the puffed grain confectionery prepared by using commercially available grain confectionery A as a control, and the results are shown in Table 5 below (however, in Table 5, 1 is very dislike, 2 is Dislike, 3 indicates moderate, 4 indicates like, 5 indicates very like).
그 결과, 상기 표 5에 나타낸 바와 같이 팽화 곡물과자의 관능평가를 수행한 결과, 색은 3.35±0.22으로 확인되었고, 맛은 3.21±0.62으로 확인되었으며, 조직감은 3.23±0.33으로 확인되었고, 향은 3.30±0.65으로 확인되어, 대조군인 A 곡물과자에 비해 관능평가 결과가 우수한 것으로 조사되었으며, 전체적인 기호도에서도 4.20±0.64로 대조군 곡물과자에 비해 우수한 것으로 확인되었다. As a result, as shown in Table 5 above, as a result of performing the sensory evaluation of the puffed grain cookies, the color was confirmed to be 3.35 ± 0.22, the taste was confirmed to be 3.21 ± 0.62, the texture was confirmed to be 3.23 ± 0.33, and the flavor was It was confirmed as 3.30±0.65, and it was investigated that the sensory evaluation result was superior to that of the control group, grain confectionery A, and the overall preference was also confirmed to be 4.20±0.64, which was superior to that of the control grain snack.
통상적으로, 제품에 있어 평점 3.5 이상이면 제품 개발에 우수함을 보이는데, 제조한 팽화 곡물과자는 4.0점을 웃도는 평점으로 소비자의 기호도에 적합하다는 사실을 확인할 수 있었다.In general, a product with a score of 3.5 or higher indicates excellent product development.
Claims (6)
(b) 곡물 입자를 수분함량이 30%가 되도록 건조하는 단계;
(c) 건조한 곡물 입자를 팽화시켜 팽화 곡물 입자를 제조하는 단계;
(d) 상기 팽화 곡물 입자를 분쇄하여 곡물 분말을 제조하는 단계; 및
(e) 상기 곡물 분말을 성형하여 과자를 제조하는 단계를 포함하는,
장내 유익 미생물인 락토바실러스 에시도필러스(Lactobacillus acidophillus), 락토바실러스 델브룩키 스페이시즈 락티스(Lactobacillus delbrueckii subsp. lactis), 스테렙토코커스 살리바리어스 스페이시즈 써모필러스(Streptococcus salivarius subsp. thermophilus) 및 비피도박테리움 비피덤(Bifidobacterium bifidum)의 생육을 증진시키는 프리바이오틱스용 팽화 곡물과자의 제조방법.(a) 2% by weight of oats, 20% by weight of brown rice, 23% by weight of white rice, and 55% by weight of corn are blended based on 100% by weight of the blending weight ratio, and pulverized grains having an average particle size of 30 to 60 mesh (mesh) preparing particles;
(b) drying the grain particles to a moisture content of 30%;
(c) swelling the dry grain particles to produce expanded grain particles;
(d) pulverizing the expanded grain particles to prepare a grain powder; and
(e) comprising the step of manufacturing the confectionery by molding the grain powder,
Intestinal beneficial microorganisms Lactobacillus acidophilus ( Lactobacillus acidophillus ), Lactobacillus delbrueckii spaces lactis ( Lactobacillus delbrueckii subsp. lactis ), Streptococcus salivarius spaces thermophilus ( Streptococcus salivarius salivarius ) And Bifidobacterium bifidum ( Bifidobacterium bifidum ) A method of manufacturing puffed grain confectionery for prebiotics that promotes the growth.
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