KR20220032878A - A method of vegetative propagation of selaginella tamariscina - Google Patents

Abstract

The present invention relates to a vegetative propagation method of Selaginella tamariscina. The vegetative propagation method of Selaginella tamariscina provided by the present invention can proliferate a large amount of high quality Selaginella tamariscina, and can induce multiple shoots, so that more Selaginella tamariscina individuals can be secured in the same period.

Description

A METHOD OF VEGETATIVE PROPAGATION OF SELAGINELLA TAMARISCINA}

The present specification relates to a method of vegetative propagation of rock son.

Vegetative reproduction is a kind of asexual reproduction, and it is a breeding method that separates parts of nutrient organs such as leaves, stems, roots, etc. from the parent of a plant to form new independent individuals. . Cutting, which is one of these vegetative propagation methods, is a breeding method in which a part of a plant is cut and inserted into sand or soil to create a new individual, and is most often used for breeding superior strains.

In the past, many studies have been conducted to increase the rooting rate of cuttings, and it is known that the rooting rate is closely related to the harvesting time of the cuttings, the age of the cuttings, the cutting site, and the cutting medium.

However, since the cutting of the rock hand has not yet been studied, research and development are required.

Republic of Korea Patent Publication No. 10-2015-0135757

In one aspect, the present invention is to provide a method for vegetative propagation of rock hand.

In one aspect, the present invention provides a method for vegetative propagation of a rock hand comprising the step of cutting the cuttings collected from the rock hand in top soil.

In an exemplary embodiment, the top soil may include top soil for horticultural use, and may further include one or more selected from the group consisting of vermiculite, perlite, and masato as an additional component.

In an exemplary embodiment, the horticultural top soil may include cocopeat, peat moss, vermiculite and zeolite.

In an exemplary embodiment, the horticultural top soil may be included in an amount of 50 wt% to 100 wt% based on the total weight of the top soil.

In an exemplary embodiment, the weight ratio of the horticultural top soil and the additional components may be 1: 0.1 to 2.

In an exemplary embodiment, the cuttings may be collected from one or more selected from the group consisting of leaves, stems, and roots of rhizomes.

In an exemplary embodiment, the cuttings may be cut to a size of 4 to 6 cm.

In an exemplary embodiment, the cuttings may be transversely cut with respect to the topsoil.

In an exemplary embodiment, the vegetative propagation method of the rock hand may further include the step of inducing rooting by irrigating the cut cuttings and maintaining them at a temperature of 18 to 28°C.

In one aspect, the vegetative propagation method provided by the present invention can proliferate a large amount of high-quality rockfish.

In another aspect, the method for vegetative propagation of basalts provided by the present invention can induce polycystic plants, so that more basalt individuals can be secured in the same period.

1 is a photograph showing the number of cuttings collected from a rock hand.
Figure 2 is a photograph showing the number of cuttings in rock hand cuttings in the horticultural soil.
3 is a photograph showing the number of rooted rock hand cuttings.
Figure 4 is a photograph showing the number of cuttings in the rock hands formed with new plants.
Figure 5 is a photograph showing the length growth of the formed polycystic plant by period after cutting.

Hereinafter, the present invention will be described in detail.

Selaginella tamariscina in the present specification is an evergreen Tathagata sea fern in the family Buddhason family, also called gwonbaek (卷柏), and many beard roots are entangled to form a stalk, and when dry, the leaf dries like a ball and there is moisture. spread out These rocks are distributed all over Korea, including Jeju Island, and are known to be effective for hemostasis, bruises, and abdominal pain.

In one aspect, the present invention provides a method for vegetative propagation of rock hand ( Selaginella tamariscina ) comprising the step of cutting the cuttings collected from the top soil.

In the present specification, cuttings refer to a vegetative propagation method in which a part of a plant's nutrient organs, such as branches, roots, and leaves, is separated from the parent body, inserted into soil or sand, and rooted to make an independent plant body.

In one embodiment, the top soil may include horticultural top soil. The horticultural top soil may include coco peat, peat moss, vermiculite and zeolite.

In one embodiment, the pH of the horticultural top soil may be 4 to 8, preferably 5 to 7.

In one embodiment, the electrical conductivity (EC) of the horticultural topsoil may be 1.2 dS/m or less, specifically 0.8 to 1.2 dS/m.

In one embodiment, the top soil may further include one or more selected from the group consisting of vermiculite, perlite, and masato as an additional component in addition to the horticultural top soil.

In one embodiment, the horticultural top soil may be included in an amount of 50 to 100 wt % based on the total weight of the top soil. When the horticultural top soil is included in less than 50% by weight, the rate of formation of multi-headed plants of cuttings is reduced. Specifically, the horticultural top soil is 50 wt% or more, 51 wt% or more, 52 wt% or more, 53 wt% or more, 54 wt% or more, 55 wt% or more, 56 wt% or more, 57 % or more, 58% or more, 59% or more, 60% or more, 61% or more, 62% or more, 63% or more, 64% or more, 65% or more, 66% or more, 67 % or more, 68% or more, 69% or more, or 70% or more, and 100% or less, 99% or less, 98% or less, 97% or less, 96% or less, 95% or less, 94 wt% or less, 93 wt% or less, 92 wt% or less, 91 wt% or less, 90 wt% or less, 89 wt% or less, 88 wt% or less, 87 wt% or less, 86 wt% or less, 85 wt% or less, It may be included in an amount of 84% by weight or less, 83% by weight or less, 82% by weight or less, 81% by weight or less, or 80% or less.

In one embodiment, the weight ratio of the horticultural top soil and the additional components may be 1: 0.1 to 2, preferably 1: 0.5 to 1.5, more preferably 1:1. If the weight ratio of the horticultural topsoil and the additional components is out of the above range, the rate of formation of mulberry plants may be reduced.

In one embodiment, the cuttings may be collected from the spores, specifically, the stems, leaves or roots of the sporophytes of the spores may be cut to a size of 4 to 6 cm.

In one embodiment, the cuttings can be cut transversely with respect to the top soil.

In one aspect, the method for vegetative propagation of rockfish according to an embodiment of the present invention may further include the step of inducing rooting by irrigating the cuttings and maintaining them at a temperature of 18 to 28°C.

Hereinafter, the present invention will be described in more detail through examples and the like. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

[Example 1] Vegetative propagation of rock hands

The above-ground part of the rock son (Yongpyeong-myeon, Pyeongchang-gun, Gangwon-do) was cut to a size of 4 to 6 cm to collect cuttings (FIG. 1 is a photograph showing the collected cuttings). The cuttings were planted using horticultural tops (Hanareum Sangto No. 2, Shinsung, Korea) as top soil for 32 seedling trays (GC-1000, Jeotech, Korea) (FIG. 2 shows the number of cuttings) is a photo). Rooting was induced by culturing the cuttings for 6 weeks at a temperature of 24 to 26 ° C and a humidity of 75 to 85% with a light intensity of 16 hours during the day (intensity: 30PPFD) and 8 hours at night (intensity of light: 0PPFD). .

[Example 2]

Nutrient propagation of the rock hands was carried out in the same manner as in Example 1, but 50 wt% of horticultural top (Hanareum Sangto No. 2, Shinsung Co., Korea) and 50 wt% of vermiculite (Verminuri, GC Co., Korea) were used as top soil. It was mixed and used.

[Example 3]

Nutrient propagation of the rock hands was carried out in the same manner as in Example 1, but 50 wt% of horticultural top (Hanareum Sangto No. 2, Shinsung Co., Korea) and 50 wt% of perlite (New Pearl Shine No. 1, GC Co., Korea) % was mixed and used.

[Example 4]

The vegetative propagation of the rock hands was carried out in the same manner as in Example 1, but 50 wt% of horticultural top (Hanareum Sangto No. 2, Shinsung Co., Korea) and 50 wt% of Masato (Gyeongju, Korea) were mixed and used.

[Comparative Example 1]

The vegetative propagation was carried out in the same manner as in Example 1, but vermiculite (Verminuri, GFC, Korea) was used as the top soil.

[Comparative Example 2]

The vegetative propagation was carried out in the same manner as in Example 1, but perlite (New Pearl Shine No. 1, GFC, Korea) was used as the top soil.

[Comparative Example 3]

In the same manner as in Example 1, vegetative propagation was carried out, but Masato (Gyeongju, Korea) was used as the top soil.

[Comparative Example 4]

Nutrient propagation was carried out in the same manner as in Example 1, but the cuttings were transplanted into Floral Foam (OASIS, Korea).

[Experimental Example 1] Evaluation of rooting rate and multi-stalk formation rate

Rooting rate and polyclinic formation rate of the cuttings cultured in Examples 1 to 4 and Comparative Examples 1 to 4 were measured, and the results are shown in Table 1 below. Specifically, in Examples 1 to 4 and Comparative Examples 1 to 4, the rooting rate was measured by considering that the length of the root was 1 mm or more for 10 individual cuttings of each of Comparative Examples 1 to 4 as rooting (FIG. 3 shows the rooted individual of Example 4) picture shown). The rate of formation of multiple shoots was measured by considering the formation of shoots of 1 mm or more in the initial cuttings as multi-crest formation (FIG. 4 is a photograph showing the individual of Example 2 in which shoots occurred).

Rooting rate (%) Polyclinic formation rate (%) Example 1 50 50 Example 2 10 30 Example 3 10 40 Example 4 10 30 Comparative Example 1 10 10 Comparative Example 2 10 10 Comparative Example 3 30 10 Comparative Example 4 0 0

[Experimental Example 2] Induction of polycystic plants

The cuttings of a rock hand harvested in the same manner as in Example 1 were placed horizontally with respect to the top soil, and the cuttings were carried out. As the top soil, horticultural top soil (Hanareum Sangto No. 2, Shinsung Corporation, Korea) was used. With a light intensity of 16 hours during the day (intensity: 30PPFD) and 8 hours at night (intensity of light: 0PPFD), the cuttings were cultured for 9 weeks at a temperature of 24 to 26 ° C and a humidity of 75 to 85%, and whether or not new shoots were grown It was observed and the results are shown in FIG. 5 .

As shown in FIG. 5 , a large number of shoots of about 1 mm were generated after 3 weeks from cutting and culturing, and it was confirmed that the shoots that occurred thereafter grew normally.

Claims (10)

A method of vegetative propagation of rock hand ( Selaginella tamariscina ), comprising the step of cutting the cuttings collected from the top soil. According to claim 1,
The top soil, including horticultural top soil, vegetative propagation method of rock son. 3. The method of claim 2,
The horticultural top soil is included in 50% to 100% by weight based on the total weight of the topsoil, vegetative propagation method of rock son. 3. The method of claim 2,
The horticultural topsoil includes coco peat, peat moss, vermiculite and zeolite, vegetative propagation method of rock son. 3. The method of claim 2,
The top soil further comprises one or more selected from the group consisting of vermiculite, perlite and masato as an additional component, vegetative propagation method of rock son. 6. The method of claim 5,
The weight ratio of the horticultural top soil and additional components is 1: 0.1 to 2, vegetative propagation method of rock son. According to claim 1,
The cuttings are collected from one or more selected from the group consisting of leaves, stems, and roots of a rock hand, a method of vegetative propagation of a rock hand. According to claim 1,
The cuttings are cut to a size of 4 to 6 cm, vegetative propagation method of rock hand. According to claim 1,
The vegetative propagation method of rock hand, in which the cuttings are transversely cut with respect to the topsoil. According to claim 1,
The vegetative propagation method of rock hand further comprising the step of inducing rooting by irrigating the cut cuttings and maintaining the temperature of 18 to 28 ° C.

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