KR20220012532A - Health food composition with significantly improved blood sugar lowering and diabetes prevention effect, and slug breeding method therefor - Google Patents

Abstract

The present invention relates to a method for breeding cicada larva with significantly improved effects of lowering blood sugar and preventing diabetes by improving a breeding method or breeding conditions for cicada larva, and various functional foods or pharmaceutical compositions having blood sugar lowering and diabetes prevention effects using cicada larva bred by the method. Provided is a method for breeding cicada larva with significantly improved effects of lowering blood sugar and preventing diabetes. The method is characterized by comprising: a step a) of roasting dried mulberry leaf powder, minced ginger, and Glycyrrhiza uralensis powder in a heated pan: a cicada larva feed producing step b) of injecting lactic acid bacteria to a resultant product obtained in the step a), fermenting the product at a temperature of 37℃ for seven days so as to obtain a solid fermented product, sterilizing the solid fermented product using steam at a temperature of 100℃ for 30 minutes, and then inhibiting the fermentation of the sterilized product so as to prepare cicada larva feed; and a step c) of breeding cicada larva using the prepared feed.

Description

Health food composition with significantly improved blood sugar lowering and diabetes prevention effect, and slug breeding method therefor {Health food composition with significantly improved blood sugar lowering and diabetes prevention effect, and slug breeding method therefor}

The present invention relates to a method for producing slugs with significantly improved blood sugar lowering and diabetes prevention effects by improving the breeding method or breeding conditions of slugs, and various functional foods having blood sugar lowering and diabetes prevention effects using the slugs produced by the method or to a pharmaceutical composition.

Recently, functional foods using insects or pharmaceutical compositions using insects as a raw material of natural products have been developed. These insects have also been used as traditional herbal medicines, and there are about 30 kinds of insects such as slugs, silkworms, cicadas, cordyceps, or centipedes.

Among them, the slug is a larva of Protaetia brevitarsis, which includes diseases caused by donguibogam and human diseases such as liver cancer, cirrhosis, hepatitis, and relief from accumulated fatigue, loss of vision, cataracts, malignant boils, stomatitis, and tetanus. It is a traditional herbal medicine that is said to be effective in treating adult diseases such as stroke, stroke, etc.

For the extract obtained by extracting the slug, there is a composition showing the effect of protecting the liver by containing a slug enzyme treatment product, an enzyme treatment product of a brown mealworm larva, an wormwood extract, a dandelion extract and a milk thistle extract in Korean Patent No. 10-1852840 Korean Patent Publication No. 10-0377252 discloses an anticancer food comprising a hot water leached extract from slugs (cicadas, scarabs, stag beetles, mulberry or moth larvae) and a hot water leached extract from Reishi mushroom. .

Looking at the prior art, the extract is obtained by treating slugs with an enzyme selected from Flavozyme, Alcalase, or Protease or leaching with hot water, thereby obtaining liver protection and anticancer effects. have.

Separately, it is widely known to the public that slugs have diabetes-related effects, and reports on their blood sugar-lowering effects are also being made (Study on Insect Proteoglycan Separation and Purification and Pharmaceutical Materialization, National Academy of Agricultural Sciences, 2013). In addition, in Korean Patent No. 10-1888549, a mixed extract obtained by combining a certain amount of slug as a main raw material with pumpkin extract, goji berry extract, baekbokryeong extract, psyllium extract, Angelica asiatica extract, and arrowroot extract according to the composition ratio is surfaced with chitosan. Disclosed is a diet drink with excellent effect of lowering blood cholesterol and triglycerides integrated by coating.

However, since there has not yet been published a method for producing slugs with significantly improved blood sugar lowering and diabetes prevention effects by improving the breeding method or breeding conditions of slugs, if such a technique is achieved, various functional foods or It is expected to be available as a pharmaceutical composition.

1. Republic of Korea Patent Publication No. 10-1852840 2. Republic of Korea Patent Publication No. 10-0377252 3, Republic of Korea Patent Publication No. 10-1888549

Accordingly, the technical problem to be achieved by the present invention is a method for producing slugs with significantly improved blood sugar lowering and diabetes prevention effects by improving the breeding method or breeding conditions of slugs, and a blood sugar lowering and diabetes prevention effect using the slugs produced by the method It is to provide a composition having a.

In order to achieve the above technical problem, the present invention

a) Roasting dried mulberry leaf powder and minced ginger and licorice powder in a heated pan:

b) In the result of step a), 5 parts by weight of lactic acid bacteria were added to the resultant, and the solid fermented product obtained by fermentation at 37° C. for 7 days was steam sterilized again at 100° C. for 30 minutes to suppress post-fermentation to produce a slug feed. a slug feed manufacturing step; and

c) breeding slugs using the prepared feed

It provides a method for rearing slugs with significantly improved blood sugar lowering and diabetes prevention effects, comprising:

In the slug breeding method according to the present invention as described above, the lactic acid bacteria may be Lactobacillus Casei .

In the slug breeding method according to the present invention as described above, it is preferable to further add the illite powder at the same weight when the lactic acid bacteria are added in step b).

In the slug breeding method according to the present invention as described above, step (c) may be a method of mixing the feed prepared in step (b) with sawdust feed for slugs and ingesting the slugs.

In the slug breeding method according to the present invention as described above, it is preferable to include 1/25 of the feed prepared in step b) in the sawdust feed for slugs relative to the weight.

In the slug breeding method according to the present invention as described above, the step (c) is a method of ingesting the feed prepared in the step (b) to the slugs while stopping the supply of the slug sawdust feed 7 to 14 days before the slugs are shipped. can be

In order to achieve another technical object of the present invention, there is provided a blood sugar lowering and diabetes prevention health food composition comprising slugs produced by the above-described breeding method.

In the health food composition according to the present invention as described above, the slugs may be included in the form of a dry powder or extract.

According to the present invention, by accumulating the blood sugar lowering and diabetes prevention effects of mulberry leaves, the blood sugar lowering and diabetes prevention effects of the mulberry leaves can be remarkably increased. Very useful.

In addition, the slugs bred according to the present invention have significantly improved functionality than general slugs and can be said to be advanced slugs, which can greatly help increase the income of slugs breeding farmers.

Hereinafter, specific contents for carrying out the present invention will be described in detail.

The present invention relates to a method and method for producing slugs with significantly improved antidiabetic effect by using mulberry leaves to produce a feed in a form in which slugs can easily accumulate, and then use the feed to breed slugs It relates to a composition using slugs produced by

The mulberry leaves used in the present invention are widely known to be effective in diabetes when ironed as tea. Recently, they are also effective in various adult diseases such as high blood pressure and arteriosclerosis. In addition, heavy metal discharge in the body, diuresis, aging inhibition, and anti-cancer action have.

The reason that mulberry leaves have various effects is because they contain various functional ingredients, and it has been analyzed that mulberry leaves already contain more than 50 kinds of various minerals.

Among them, as shown in the following table, mulberry leaves were found to have a very high content of calcium, potassium, and iron, and 24 amino acids, which are components of proteins, such as methionine and alanine, were abundantly contained. It was found to have the highest protein content after soybeans.

In addition, mulberry leaves contain glutamine, which forms bones and coagulates blood with calcium, and aspartic acid and arginine, which eliminate hangovers, and are obtained when protein is hydrolyzed. It is also known to contain Serine, which prevents senile dementia, and Glutathione, an antioxidant that protects cells from aging.

The composition and general chemical composition of the major functional ingredients contained in these mulberry leaves are as follows.

Nutrients of mulberry leaves (mg/dried leaves 100g)

General chemical composition of mulberry leaves

Among the nutrients contained in the mulberry leaf, calcium is 6 times that of green tea and milk, iron is 3 times that of spinach, and dietary fiber is 4.7 times that of green tea. is known

In addition, among the functional ingredients recently found to be contained in mulberry leaves, Ruthin has the effect of strengthening capillaries, and GAVA, Moracenin, and Sanggenine have a blood pressure lowering effect. Moracin, Dimoracin, and Chalcomoracin have antibacterial activity, Umbelliferone has an anti-inflammatory effect, and Morusin has an antitumor effect. Oxynojirimycin (1-deoxinojirimycin), Fagomine, iminoarabinitol (1,4-dideoxy-1,4-imino-D-arabinitol), alpha-glucosidase inhibitor (α -Glucosidase inhibitors, such as Nojirimycin and Calystegin, have also been found to be effective in diabetes.

Regarding such mulberry leaves, the Korean traditional medical book 『Donguibogam』 states, “Mulberry leaves are warm and non-toxic. It is written, "Bonchogangmok (本草綱目)" says "Mulberry leaves are sore from small thirst, wind, bruises and wind, snake and insect bites, night sweats, blue blindness" It is effective for symptoms such as hair growth, shin failure, prolapse, boils or sores, nail punctures, burns, numbness and numbness in the hands and feet.” has been

In the present invention, after drying such mulberry leaves into powder, they are roasted without oil in a heated pan together with minced ginger and licorice powder, cooled, and then 5 parts by weight of lactic acid bacteria are added to 100 parts by weight of roasted ginger and mulberry leaf powder at a temperature of 36 ° C. Fermentation treatment is performed for 7 days.

At this time, there is no need to supply moisture, and the moisture required for microbial fermentation is sufficient as the moisture absorbed by the material itself during the autoclave sterilization process, so a separate supply is not required.

The most preferred lactic acid bacteria for obtaining the fermented feed of the present invention was Lactobacillus Casei .

The solid fermented feed raw material of the present invention fermented by the fermenting strain of the present invention is sterilized by steam heat treatment at 100° C. for 30 minutes.

In addition, it is very advantageous to increase the efficiency of lactic acid bacteria fermentation and slug breeding efficiency to add together with the illite powder of the same weight as the weight of the lactic acid bacteria when adding lactic acid bacteria.

Illite used in the present invention was first discovered in Illinois in the United States in 937, and as a rare mineral with the largest reserves in Yeongdong-gun, North Chungcheong Province, the components of Illite are silicon dioxide (SiO ₂ ), alumina ( Ai ₂ O ₃ ), iron oxide (Fe ₂ O ₃ ), calcium oxide (CaO), sodium oxide (Na ₂ O), magnesium oxide (MgO), TiO ₂ , MnO and K ₂ O It has effects such as sebum removal, blood circulation promotion, collagen binding, detoxification, and moisture control.

It was confirmed that the slug feed prepared according to the present invention has no problem in breeding slugs, and that the slugs more easily accumulate the blood sugar lowering and diabetes prevention substances contained in the feed.

Next, a method for breeding slugs using the prepared feed will be described.

The method for rearing slugs in the present invention can be divided into two. The first method is to mix and supply the feed according to the present invention to the known sawdust feed used for rearing slugs throughout the slug breeding period.

At this time, it was found that the amount of feed supplied was optimal for various tests of the present inventors to put about 1/25 of the known sawdust feed and weight.

Another method is to ingest the slugs while stopping the supply of sawdust feed to the slugs before manufacturing them as health food, that is, 7 to 14 days before shipment. In this case, the slugs are transferred to a breeding barrel without sawdust and only the feed prepared according to the present invention is fed to the slugs. In this case, it is preferably fed for about 10 days. It is highly desirable that the lowering and antidiabetic substances accumulate more easily.

Hereinafter, it will be described in detail through Examples and Experimental Examples.

< Example 1>

After adding 15 parts by weight of minced ginger and licorice powder to 100 parts by weight of dry mulberry leaf powder, roasting, and cooling, 5 parts by weight of Lactobacillus Casei were added, followed by fermentation at 36° C. for 7 days. It was prepared by sterilization by steam heating at ℃ for 30 minutes.

. < Example 2>

It is the same as that of Example 1, except that illite powder was further added in the same weight ratio as lactic acid bacteria.

<bringing method>

Then, by preparing four breeding barrels, the first test group 1 ingested the feed of Example 1, the second test group 2 ingested the feed of Example 2, and the third comparison ingestion of the mulberry leaf dry powder as feed The fourth group was a control group that consumed general sawdust feed, and the number of months of slugs was the same in each breeding bin, and the weight was also selected as the same, and divided and bred in each breeding bin 10 days before shipment.

Before the manufacture of health food, the weight of the slugs did not differ significantly depending on the breeding method, and the slugs were fasted for two days and then prepared by freeze-drying.

< test example 1: In vitro enzyme activity α- glucosidase inhibitory activity>

The α-Glucosidase inhibition assay method was measured using the method of Tibbot and Skadsen (1996), etc. The enzyme was α-glucosidase (sigma, USA) obtained from yeast, and the substrate was p-nitrophenyl-α-D-glucopyranoside (Sigma). , USA) were used. α-Glucosidase was used as an enzyme solution by dissolving 0.7 units in 100 mM phosphate buffer (pH 7.0) containing 0.2% BSA and 0.02% NaN3, and p-Nitrophenyl-α-D-glucopyranoside was used as an enzyme solution in 100 mM phosphate buffer (pH 7.0). ) was dissolved to 10 mM and used as a substrate solution. After that, 50 μl of the ethanol extract sample obtained by ethanol extraction of the freeze-dried slugs was added to a microplate, 100 μl of α-glucosidase enzyme was taken, and then incubated at room temperature for 5 minutes at 25 ° C. Multi Detection Reader (Infinite 200, TECAN Group Ltd, Switzerland) Absorbance was measured at 405 nm.

Then, 50 μl of the substrate solution was added, and after 2 minutes, the absorbance was measured at 405 nm with a multi-detection reader to calculate the enzyme inhibition rate, and the results are shown in Table 1.

[Equation 1]

Inhibitory activity (%)=(control absorbance)-(sample treatment group absorbance)/(control absorbance)X100

division
(unit: %) Concentration (μg/ml) 0 10 100 1000 10000 test group 1 0 1.5 7.9 15.2 27.2 test group 2 0 1.9 9.1 18.8 29.6 control group 0 0.6 3.8 9.8 11.1 control 0 0.4 2.4 7.1 10.3

As shown in Table 1 above, the test group of the present invention showed a significantly improved effect than the comparison group and the control group, there was a difference between the comparison group and the control group, and it was difficult to find a special meaning. was confirmed to be unable to breed slugs, which are useful for lowering blood sugar and preventing diabetes.

< Experimental example 2: in in vivo Animal Testing>

test animal

ICR mice (6 weeks old, male), the test animals, were purchased from Samtaco Bio Korea (Gyeonggi, Korea) and used in the experiment after undergoing a one-week acclimatization period. For the experimental diet, general solid feed (Sam Taco, Gyeonggi, Korea) was fed, and the filtered drinking water was changed every day and the drinking water was freely consumed. During the breeding period, the temperature is 23±1℃, humidity 50±5%, noise 60 phone or less, lighting time 08:00~20:00 (12 hours a day), illumination 150~300 Lux, ventilation 10~12 times per hour environment was maintained.

After measuring the fasting blood glucose of test animals that have been fasted for more than 8 hours using a blood glucose meter (AutoCheck, Diatech Korea, Inc.) in the OGTT caudal vein, they were randomly assigned to each group using the egg mass method. Then, 100mg/kg of dry powder of each slug was orally administered. After 30 minutes, blood glucose was measured again, and according to the experimental method, glucose, which is a blood glucose-increasing factor, was administered to all groups by 2g/kg at intervals of 30 minutes. Blood glucose was measured in the caudal vein up to 120 minutes, and the results are shown in Table 2 below.

division hours (minutes) -30 0 30 60 100 120 test group 1 84.17
±8.89 122.94±10.12 354.73±38.22 222.34±38.45 149.87±11.92 115.22±12.21 control group 85.57
±7.46 135.86±14.23 392.71±53.22 247.14±35.86 187.57±16.47 132.86±16.10 control 85.34±7.66 137.32±9.22 399.27±40.12 255.17±35.28 194.85±20.17 142.38±10.33

As shown in Table 3, the test group of the present invention showed a significantly improved effect than the comparison group and the control group, there was a difference between the comparison group and the control group, and it was difficult to find a special meaning. It was confirmed that it was impossible to breed slugs useful for lowering blood sugar and preventing diabetes.

As described above, according to the present invention, the blood sugar lowering and diabetes preventive effects of mulberry leaves can be accumulated to remarkably increase the blood sugar lowering and diabetes preventive effects of the slugs basically, and using this to manufacture food or pharmaceuticals, diabetes mellitus very useful in the prevention and treatment of

In addition, the slugs bred according to the present invention have significantly improved functionality than general slugs, and can be said to be advanced slugs, which can greatly help increase the income of slugs breeding farms.

Claims (8)

a) Roasting dried mulberry leaf powder and minced ginger and licorice powder in a heated pan:
b) In the result of step a), 5 parts by weight of lactic acid bacteria were added to the result, and the solid fermented product obtained by fermentation at 37° C. for 7 days was steam sterilized again at 100° C. for 30 minutes to suppress the fermentation to produce a slug feed. a slug feed manufacturing step; and
c) breeding slugs using the prepared feed
A method for rearing slugs with significantly improved blood sugar lowering and diabetes prevention effects, comprising:
The method of claim 1,
A method for breeding slugs with significantly improved blood sugar lowering and diabetes prevention effects, characterized in that the lactic acid bacteria is Lactobacillus Casei .
3. The method according to claim 1 or 2,
A method for breeding slugs with significantly improved blood sugar lowering and diabetes prevention effects, characterized in that the illite powder is further added with the same weight when lactic acid bacteria are added in step b).
3. The method according to claim 1 or 2,
The step (c) is a method in which the feed prepared in step (b) is mixed with sawdust feed for slugs and ingested by the slugs.
5. The method of claim 4,
A method for breeding slugs with significantly improved blood sugar lowering and diabetes prevention effects, characterized in that the feed prepared in step b) is contained in the sawdust feed for slugs by 1/25 by weight.
3. The method according to claim 1 or 2,
The step (c) is characterized in that the feed prepared in the step (b) is ingested to the slugs while stopping the supply of sawdust feed to the slugs 7 to 14 days before the slugs are shipped. An improved method of rearing slugs.
A health food composition for lowering blood sugar and preventing diabetes, comprising the slug according to any one of the preceding claims.
The health food composition for lowering blood sugar and preventing diabetes according to claim 7, wherein the slugs are contained in a dry powder or extract form.