KR20210152411A - Food Composition Comprising the Extract of Chaenomelis langenariae radix for Improving Skin - Google Patents

Abstract

The present invention relates to a food composition containing a chaenomelis langenariae radix extract as an active ingredient and used for skin improvement, skin aging prevention, skin moisturizing, skin wrinkle improvement, ultraviolet skin damage suppression, or skin protection from UV rays, a health functional food containing the food composition, a pharmacological composition containing a chaenomelis langenariae radix extract as an active ingredient and used for skin wrinkle or dry skin prevention or treatment or ultraviolet skin damage prevention or suppression, and a cosmetic composition and a quasi-drug composition for skin improvement containing a chaenomelis langenariae radix extract as an active ingredient. The chaenomelis langenariae radix extract of the present invention is capable of cell protection from ultraviolet rays, inhibition of MMP-1 expression increased by UV irradiation, post-decrease procollagen content increase, and moisture retention increase. Accordingly, the composition containing the chaenomelis langenariae radix extract is useful for skin improvement, skin aging prevention, skin moisturizing, skin wrinkle improvement or prevention, ultraviolet skin damage suppression, or skin protection from UV rays.

Description

Food Composition Comprising the Extract of Chaenomelis langenariae radix for Improving Skin

The present invention relates to a food composition for skin improvement, skin moisturizing, skin wrinkle improvement, skin damage suppression due to ultraviolet rays or skin protection from ultraviolet rays comprising an extract of the corresponding root extract as an active ingredient; Health functional food containing the food composition; A pharmaceutical composition for preventing or treating skin wrinkles or dry skin, or preventing or inhibiting skin damage caused by ultraviolet rays, comprising an extract of corresponding root, as an active ingredient; And it relates to a cosmetic composition for skin improvement and a quasi-drug composition comprising the extract of the corresponding root as an active ingredient.

Collagen, a major component of the extracellular matrix, is a major matrix protein produced by fibroblasts of the skin. In addition, it is an important protein accounting for about 30% of the total weight of biological proteins and has a strong heavy helix structure. Collagen forms most of the organic substances of skin, tendons, bones and teeth, and the content thereof is particularly high in bones and skin (dermis). It exists as a fibrous inclusion body in most other body structures.

Collagen is a relatively weak immunogen, partly due to the masking of latent epitope by its helix, which also renders the collagen resistant to proteolysis. As the main functions of collagen, it is known that the mechanical firmness of the skin, the resistance of the connective tissue and the binding force of the tissue, the support of cell adhesion, and the induction of cell division and differentiation (in the case of growth of an organism or wound healing) are known (Van der Rest et al. ., Ann NY Acad Sci, 1990).

Such collagen decreases with age and photoaging by UV irradiation, which is known to be closely related to the formation of wrinkles in the skin (Arthur K. Balin et al., Aging and the skin, 1989). In addition, collagen plays an important role in wound healing, and promotes the synthesis of collagen in the damaged epithelium so that the wound can be restored quickly and without scarring.

Conventionally, in order to take advantage of the skin moisturizing and wound healing effects of collagen, products containing collagen in a composition for external application for skin, such as cosmetics or ointments, have been released. It cannot be said that it exhibits essential skin function improvement and wound healing functions because it cannot be expected to have moisturizing or wound healing effects due to its difficulty in transdermal absorption.

On the other hand, human skin is affected in various ways by exposure to UV rays. UV rays irradiated to the skin are either directly reflected at the boundary of the skin layer (ie, epidermal reflection, dermal reflection) or after striking cells, fibers or particles within the skin tissue. Scattered, absorbed, or transmitted through one of four pathways. The double absorbed ultraviolet rays cause a photochemical reaction in the skin, thereby causing skin lesions. In particular, UVA (UVA) having a long wavelength and low energy penetrates to the dermis of the skin and promotes skin cancer, wrinkles, and melanin formation, etc. It is known to cause skin aging and skin irritation. UVB) is known to be the main cause of damage to the skin such as erythema, freckles and edema by penetrating into the epidermis of the skin because of its high energy intensity. In addition, chronic exposure to the above 　 UV rays 　 B results in skin aging, immune suppression, skin cancer and cell death.

Recently, in order to suppress such damage to the skin and the skin caused by UV rays, a UV blocker that blocks the penetration of UV rays to protect cells and a cosmetic composition using ingredients with an antioxidant function that inhibits the generation of intracellular reactive oxygen species by UV rays Development is actively taking place. However, most of the developed skin protection agents against UV rays have synthetic compounds as active ingredients, so they have disadvantages in that they cause damage to the skin or cause side effects such as itching and spots. Therefore, there is a need to develop a new natural material that has no side effects on the skin and has excellent activity in inhibiting skin damage and protecting the skin caused by UV rays.

Under this background, the present inventors studied intensively to develop a natural material that can exhibit the effects of skin improvement, skin aging prevention, skin moisturizing, skin wrinkle improvement or prevention, suppression of skin damage by ultraviolet rays, or skin protection from ultraviolet rays without side effects As a result of efforts, the present invention was completed by confirming that the Korean root extract exhibits skin improvement, skin aging prevention, skin moisturizing or wrinkle improvement effects, as well as skin damage inhibition and skin protection effects from UV rays.

Republic of Korea Patent Publication No. 10-2019-0028996

It is an object of the present invention to provide a food composition for skin improvement comprising an extract of the corresponding root of the present invention.

Another object of the present invention is to provide a health functional food comprising the food composition.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating skin wrinkles or dry skin, or preventing or inhibiting skin damage caused by ultraviolet rays, comprising an extract of the corresponding root of the present invention as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for improving skin comprising an extract of the corresponding root of the present invention.

Another object of the present invention is to provide a quasi-drug composition for skin improvement comprising an extract of the corresponding root of the present invention as an active ingredient.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present application may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in the present application fall within the scope of the present application. In addition, it cannot be seen that the scope of the present application is limited by the detailed description described below.

As an aspect for achieving the above object, the present invention is a food composition for improving skin comprising an extract of the corresponding root as an active ingredient; And it provides a health functional food comprising the food composition.

As used herein, the term "carrot root (Chaenomelis langenariae radix)" is the root part of the flower, and it is a deciduous broad-leaved shrub growing at the shore or at the foot of a mountain. The root extract is known as a herbal extract that is safe for the human body as it not only has the effect of lowering hypertriglyceridemia and hepatocellular protection, but also has very weak toxicity.

The term "extract" of the present invention refers to an extract obtained by the extraction treatment of the corresponding root, a diluted solution or a pesticide of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc., It includes extracts of all formulations that can be formed using the extract itself and the extract.

The method of extracting the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction method, cold extraction method, solvent extraction method, steam distillation method, elution method, compression method, ultrasonic extraction method, filtration method, reflux extraction method, etc. can be performed.

The extract of the present invention is extracted from the corresponding root using an appropriate solvent, and may include, for example, a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract. The type of extraction solvent used to prepare the extract is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; lower alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propanol, isopropanol, butanol, propyl alcohol, and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used. In addition, it is possible to prepare a solvent extract by extracting one or more times using the solvent.

The extract of the present invention can be used as it is after filtering the particles using filtration, for example, using nylon, or using a freeze filtration method to remove floating solid particles, or freeze-drying, hot air drying, spray drying, etc. It can be used after drying. In addition, the extract may be further subjected to a conventional fractionation process, and may be purified using a conventional purification method.

As used herein, the term “active ingredient” refers to a component capable of exhibiting the desired activity alone, or in combination with a carrier that has no activity by itself.

As used herein, the term “improvement” refers to any action that improves or benefits the skin by using the glycolytic root extract in the present invention.

The improvement of the skin condition of the present invention is to prevent skin aging; moisturizing the skin; improving or preventing skin wrinkles; inhibiting, improving or preventing skin damage caused by UV rays; Or it may include, but is not limited to, skin protection from UV rays.

The term "skin aging prevention" of the present invention prevents chronological aging (endogenous aging), which is a natural aging that occurs due to physiological changes in the body over time, and photoaging (photogenic aging) that occurs in areas exposed to sunlight Or all that delay.

As used herein, the term "skin moisturizing" means to increase the feeling of moisture in the skin and to maintain a moist state. If the skin 　 moisturizing effect is increased, 　 may have a beneficial effect on the improvement of wrinkles and increase of elasticity of the skin.

As used herein, the term "improving or preventing skin wrinkles" means inhibiting or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles already formed.

The term "ultraviolet radiation" in the present invention refers to light with a wavelength range of 10 to 400 nm (energy range 3 eV to 124 eV), and is a generic term for electromagnetic waves whose wavelength is shorter than visible light and longer than X-rays. (320-400 nm), 　UV-B (280-320 nm), 　UV-C (100-280 nm).

In the present invention, ultraviolet rays may be at least one selected from the group consisting of UVA, UVB and UVC, and specifically, may be a mixture of UVA and UVB, but is not limited thereto.

As used herein, the term "skin damage due to ultraviolet rays" refers to all symptoms that occur as the skin is exposed to ultraviolet rays, and is used in a broad sense including skin photoaging.

If the skin damage caused by the ultraviolet rays of the present invention is damage or symptoms that occur as the skin is exposed to ultraviolet rays, the specific type or severity of the symptoms is not particularly limited, for example, skin damage caused by ultraviolet rays is not particularly limited. It may be wrinkle generation, reduced skin elasticity, photoaging or skin keratinization, but is not limited thereto.

As used herein, the term “skin protection” refers to preventing skin damage, inflammation, dryness, etc. caused by external stimuli or aging, and improving the skin.

inhibiting, ameliorating or preventing skin damage in the present invention; Alternatively, skin protection is achieved by protecting skin cells from UV rays, suppressing the expression of collagen degrading enzymes such as MMP-1, promoting collagen synthesis in the skin, enhancing skin moisture, improving skin wrinkles, improving skin photodamage, or inhibiting skin photoaging, However, the present invention is not limited thereto.

Specifically, in the experimental example of the present invention, the glycolytic root extract has a skin moisturizing effect, a skin cell protection effect from UV rays, an MMP-1 (Matrix Metalloproteinase-1) expression inhibitory effect, and a pro-collagen expression promoting effect. It was confirmed that it can be usefully used for skin improvement, skin aging prevention, skin moisturizing, skin wrinkle improvement or prevention, suppression of skin damage caused by UV rays, or skin protection from UV rays.

The food composition of the present invention may contain the corresponding root extract in various amounts as long as it has the effect of improving skin, preventing skin aging, moisturizing the skin, improving or preventing skin wrinkles, inhibiting skin damage caused by ultraviolet rays or protecting the skin from ultraviolet rays. have.

The food composition of the present invention may further include any compound or natural extract known to have corresponding activity and safety has already been verified in the art in order to enhance the convenience of taking or ingestion, in addition to the glycolytic root extract. .

Such compounds or extracts include compounds or extracts and pharmaceuticals listed in compendial documents such as pharmacopeias of each country ('Korean Pharmacopoeia' in Korea) and health functional food regulations of each country ('health functional food standards and specifications', announced by the Ministry of Food and Drug Safety in Korea). Each country's laws governing the manufacture and sale of compounds, extracts, and health functional foods that have been approved for items in accordance with the laws of each country that regulate manufacturing and sales ('Pharmaceutical Law' in Korea) ('Health Functional Food Act' in Korea) Depending on the functionalities recognized compounds or extracts may be included.

As used herein, the term "food" refers to meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, There are vitamin complexes, nutritional supplements, functional foods, food additives, health functional foods, health foods, and health foods, and includes all foods in a normal sense.

The health food means a food having an active health maintenance or promotion effect compared to general food, and the health supplement food means a food for the purpose of health supplementation. In some cases, the terms health functional 　 food, health food, and health supplement food are preferred.

As used herein, the term "health functional food" is the same term as food for special health use (FoSHU), and it is a food with high medical and medical effects processed to efficiently exhibit bioregulatory functions in addition to nutrition supply. it means. Here, "function (sex)" means to obtain a useful effect for health purposes such as regulating nutrients or physiological action with respect to the structure and function of the human body. The food of the present invention can be manufactured by a method commonly used in the art, and during the preparation, raw materials and components commonly added in the art can be added. In addition, if the formulation of the above 　 food is also a formulation that is recognized as a food, it can be manufactured without limitation. The 　food 　 composition of the present invention can be prepared in various types of dosage forms, and unlike general drugs, 　 food as a raw material has the advantage of not having side effects that may occur during long-term administration of the drug, and has excellent portability, Food can be ingested as a supplement for improving skin, preventing skin aging, moisturizing the skin, improving or preventing skin wrinkles, suppressing skin damage caused by UV rays, or protecting the skin from UV rays.

Specifically, the health function 　 food is a food prepared by adding the corresponding root extract to food materials such as beverages, teas, spices, gum, and confectionery, or manufactured in pills, tablets, capsules, powders, suspensions, etc. It means to bring a specific effect, but unlike general drugs, it has the advantage that there are no side effects that may occur when taking the drug for a long period of time by using food as a raw material.

Because the food composition of the present invention can be consumed on a daily basis, it has the effect of improving skin, preventing skin aging, moisturizing the skin, improving or preventing skin wrinkles, inhibiting, improving or preventing skin damage caused by ultraviolet rays, or protecting the skin from ultraviolet rays. It can be expected that it is very useful.

The food composition of the present invention may be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, and ionic beverages, processed oils such as milk and yogurt, gums, rice cakes, Korean sweets, bread, It can be prepared into foods such as confectionery and noodles, tablets, capsules, pills, granules, liquids, powders, pieces, pastes, syrups, gels, jellies, and preparations such as bars. In addition, the food composition of the present invention may have any product classification in terms of legal and functional classification as long as it conforms to the enforcement laws at the time of manufacturing and distribution. For example, it is a health functional food according to the 'Health Functional Food Act' of Korea, or confectionery, tea, beverage, and special food according to each food type according to the Food Ordinance of the Korea 'Food Sanitation Act' (the 'Food Standards and Specifications' notified by the Ministry of Food and Drug Safety). It may be used food and the like.

In addition, the food composition of the present invention may contain food additives in addition to the active ingredient. Food additives can be generally understood as substances that are added and mixed or infiltrated into food in manufacturing, processing, or preserving food. Food additives with guaranteed safety are limited in terms of ingredients or functions in the Food Additives Ordinance in accordance with the laws of each country that regulate the manufacture and distribution of food ('Food Sanitation Act' in Korea). In the Korean Food Additives Code, 'Food Additive Standards and Specifications' announced by the Ministry of Food and Drug Safety, food additives are classified into chemically synthetic products, natural additives, and mixed preparations in terms of ingredients. It is divided into agents, preservatives, emulsifiers, acidulants, thickeners, etc.

The sweetener is used to impart a suitable sweetness to food, and natural or synthetic ones may be used. Preferably, a natural sweetener is used. Examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

The flavoring agent may be used to improve taste or flavor, and both natural and synthetic ones may be used. Preferably, it is a case where a natural thing is used. In the case of using a natural product, the purpose of nutritional enhancement in addition to flavor may be concurrently used. The natural flavoring agent may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, or the like, or obtained from green tea leaves, horseradish leaves, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo can be used. The natural flavoring agent may be a liquid concentrate or a solid extract. In some cases, a synthetic flavoring agent may be used, and the synthetic flavoring agent may include esters, alcohols, aldehydes, terpenes, and the like.

Calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like can be used as the preservative, and as the emulsifier, acacia gum, carboxymethyl cellulose, xanthan gum, Pectin may be used, and acidulant, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like may be used. The acidulant may be added so that the food composition has an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste.

As the thickener, a suspending agent, a settling agent, a gel-forming agent, a bulking agent, and the like may be used.

The food composition of the present invention may contain, in addition to the food additives described above, physiologically active substances or minerals known in the art for the purpose of supplementing and reinforcing functionality and nutrition and guaranteed stability as food additives. Examples of the physiologically active substances include catechins included in green tea, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, and the like, and minerals include calcium preparations such as calcium citrate, stearic acid Magnesium preparations, such as magnesium, iron preparations, such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, etc. are mentioned.

The food composition of the present invention may contain the above-mentioned food additives in an appropriate amount to achieve its purpose depending on the type of product, and with respect to other food additives that may be included in the food composition of the present invention, You can refer to the Food Additives Ordinance.

In the case of using the glycolytic root extract as a food additive, it may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of its use.

As another aspect for achieving the above object, the present invention provides a pharmaceutical composition for preventing or treating skin wrinkles or dry skin, or preventing or inhibiting skin damage caused by ultraviolet rays, comprising an extract of the corresponding root extract as an active ingredient.

The terms "carrot", "extract", "active ingredient" and "ultraviolet light" in the present invention are the same as described above.

As used herein, the term "skin damage due to ultraviolet rays" refers to all symptoms that occur as the skin is exposed to ultraviolet rays, and is used in a broad sense including skin photoaging. Therefore, if the skin is damaged or symptomatic as a result of exposure to ultraviolet rays, the specific type or severity of the symptoms is not particularly limited, and for example, wrinkles caused by ultraviolet rays, reduced skin elasticity, photoaging, sunburn, It may be erythema, skin inflammation, photoallergy, phototoxicity, photosensitization, and skin keratinization, but is not limited thereto.

As used herein, the term "dry skin" refers to all symptoms caused by dry skin due to insufficient moisture in the skin.

As long as the dry skin of the present invention is a symptom caused by dry skin due to insufficient moisture in the skin, the specific type or severity of the symptom is not particularly limited.

As used herein, the term “prevention” refers to any act of inhibiting or delaying skin damage caused by UV rays by administering the pharmaceutical composition of the present invention to an individual.

As used herein, the term “treatment” refers to any action that improves, alleviates, or benefits skin wrinkles, dry skin, or skin damage caused by UV rays by administering or applying the pharmaceutical composition of the present invention to an individual.

The pharmaceutical composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent according to a conventional method. Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or formulation, and specifically, reference may be made to the pharmacopoeia of each country including the 'Korean Pharmacopoeia'. Carriers, excipients and diluents that may be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition, carriers, excipients and diluents that may be included in the composition of the present invention may be non-natural carriers, but are not limited thereto.

The pharmaceutical composition of the present invention may be formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, or sterile injection solutions according to conventional methods. . Specifically, it can be prepared using a diluent or excipient such as a filler, extender, binder, wetting agent, disintegrant, surfactant, etc. usually used when formulating. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient, for example, starch, calcium carbonate, sucrose, lactose, gelatin, etc. to the compound. It can be prepared by mixing. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid formulations for oral administration include suspensions, internal solutions, emulsions, and syrups. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives are included. can Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, Witepsol, Macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, etc. can be used. Specific formulations of pharmaceutical compositions are known in the art, and reference may be made to, for example, Remington's Pharmaceutical Sciences (19th ed., 1995). This document is considered a part of this specification.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. The pharmaceutically effective amount means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects, and the effective dose level is determined by the patient's health condition, disease type, severity, The activity of the drug, the sensitivity to the drug, the administration method, administration time, administration route and excretion rate, treatment period, factors including drugs used in combination or concurrently, and other factors well known in the medical field may be determined according to factors. Dosage and frequency do not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention may be administered to mammals such as rats, dogs, cats, cattle, horses, pigs, and humans through various routes, preferably humans. Any mode of administration may be contemplated and may be administered, for example, by oral, intravenous, intramuscular or subcutaneous injection, but is not limited thereto.

As another aspect for achieving the above object, the present invention provides a cosmetic composition for improving skin comprising an extract of the corresponding root extract as an active ingredient.

The terms "carrot", "extract" and "active ingredient" of the present invention are the same as described above.

As used herein, the term “improvement” refers to any action that improves or benefits the skin by using the glycolytic root extract in the present invention.

The improvement of the skin condition of the present invention is to prevent skin aging; moisturizing the skin; improving or preventing skin wrinkles; inhibiting, improving or preventing skin damage caused by UV rays; Or it may include, but is not limited to, skin protection from UV rays.

The terms "skin aging prevention", "skin moisturizing", "skin wrinkle improvement or prevention", "ultraviolet rays", "skin damage caused by ultraviolet rays" and "skin protection" in the present invention are the same as described above.

The cosmetic composition of the present invention can improve the skin, prevent skin aging, moisturize the skin, improve or prevent skin wrinkles, inhibit, improve or prevent skin damage caused by ultraviolet rays, or as long as it has the effect of protecting the skin from ultraviolet rays, content may be included.

The cosmetic composition according to the present invention includes various components generally used in cosmetic compositions, for example, a water-soluble component, a powder component, an oil component, a surfactant, It may be constituted by mixing a moisturizer, a viscosity modifier, a preservative, an antioxidant, a fragrance, a color, and the like.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nourishing lotion, flexible lotion, pack, soft water, emulsion, makeup base, essence, soap, liquid detergent, bath agent, sunscreen cream, sun oil, suspension, emulsion Liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray can be prepared in a formulation selected from the group consisting of, but limited thereto no.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers to be formulated in general skin cosmetics, and conventional ingredients include, for example, oil, water, surfactant, humectant, lower alcohol, thickener, A chelating agent, a colorant, a preservative, a fragrance, etc. may be appropriately mixed, but the present invention is not limited thereto. The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

As another aspect for achieving the above object, the present invention provides a quasi-drug composition comprising an extract of the corresponding root extract as an active ingredient.

The terms "carrot", "extract", "active ingredient" and "improvement" in the present invention are the same as described above.

The term "quasi-drug" as used in the present invention refers to articles with a milder action than pharmaceuticals among articles used for the purpose of diagnosing, treating, improving, alleviating, treating, or preventing diseases of humans or animals. For example, according to the Pharmaceutical Affairs Act of Korea Quasi-drugs exclude products used for pharmaceutical purposes, and include products used for the treatment or prevention of diseases in humans and animals, and products with minor or no direct action on the human body.

The improvement of the skin condition of the present invention is to prevent skin aging; moisturizing the skin; improving or preventing skin wrinkles; inhibiting, improving or preventing skin damage caused by UV rays; Or it may include, but is not limited to, skin protection from UV rays.

The terms "skin aging prevention", "skin moisturizing", "skin wrinkle improvement or prevention", "ultraviolet rays", "skin damage caused by ultraviolet rays" and "skin protection" in the present invention are the same as described above.

The quasi-drug composition of the present invention can improve the skin, prevent skin aging, moisturize the skin, improve or prevent skin wrinkles, inhibit, improve or prevent skin damage caused by ultraviolet rays, or as long as it has the effect of protecting the skin from ultraviolet rays, content may be included.

The quasi-drug composition of the present invention may be prepared in the form of an ointment, lotion, spray, patch, cream, powder, suspension, gel, or filter filler, and specifically, disinfectant, wet tissue, solid soap, liquid soap, hand It may be a detergent or hand sanitizer, but is not limited thereto.

When the composition for skin improvement according to the present invention is used as a quasi-drug 　 additive, the composition can be added as it is or used together with other quasi-drugs or quasi-drugs ingredients, and can be used appropriately according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use.

According to the present invention, the root extract has a cytoprotective effect from UV rays, and has an effect of inhibiting MMP-1 expression increased by UV irradiation and increasing procollagen content. In addition, through animal experiments, the glycolytic root extract increases skin moisture, reduces skin thickness, reduces wrinkle area and length, regulates the expression of skin wrinkle-related proteins (TMP1 increase; MMP1, MMP3 and MMP9 decrease; Pro-COL1A1 increase) and skin Moisturizing-related protein expression regulation (increased expression of hyaluronic acid; decreased HYAL1 and HYAL2; increased HAS1, HAS2 and HAS3, increased filaggrin) was confirmed to have an effect, the composition containing the glycosporidium extract is for skin improvement; for skin aging; for moisturizing the skin; for improving or preventing skin wrinkles; For inhibiting, improving or preventing skin damage caused by UV rays; Alternatively, it may be usefully used for skin protection from ultraviolet rays.

1 is a result of confirming the cell protective effect of the root extract according to the present invention in an ultraviolet irradiation environment.
Figure 2 is a result confirming the effect of the extract of the corresponding root according to the present invention on the expression of MMP-1 according to ultraviolet irradiation.
3 is a result confirming the effect of the extract of the corresponding root according to the present invention on the expression of procollagen according to ultraviolet irradiation.
Figure 4 is the result of confirming the effect of the corresponding root extract according to the present invention on the weight change of the mouse irradiated with UV light.
Figure 5 is a result confirming the effect of the extract of the corresponding root according to the present invention on the serum AST activity (Fig. 5a) and serum ALT activity (Fig. 5b) of mice irradiated with UV light.
6 is a result confirming the effect of the corresponding root extract according to the present invention on the moisture content of the skin of the mouse irradiated with ultraviolet rays.
7a to 7c are results of confirming the effect of the corresponding root extract according to the present invention on changes in skin thickness, skin wrinkle area, and skin wrinkle length of mice irradiated with UV rays, respectively.
8A and 8B are the results of histologically confirming the effect of the extract of the glycolytic root according to the present invention on the skin of a mouse irradiated with ultraviolet rays.
9 is a result of confirming the expression level of a protein related to skin wrinkle generation, FIG. 9a is a result of confirming the expression level of MMP1, MMP3, MMP9 and Pro-COL1A1, FIG. 9b is a result of confirming the expression level of TIMP1.
10 is a result of confirming the expression level of skin moisturizing-related protein, FIG. 10a is HAS1, HAS2, HAS3 and filaggrin, FIG. 10b is HYAL1 (Hyaluronidase 1), FIG. 10c is HYAL2 (Hyaluronidase 2) and FIG. 10d is the result of confirming the expression level of hyaluronic acid (hyaluronan).

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1: Preparation of an extract of Glycolyte root

The corresponding root used for this experiment was purchased from the market and chopped and pulverized. After adding 70% fermented alcohol 10 times the weight of the corresponding root to the extraction machine along with the crushed Korean root, reflux cooling extraction was performed by repeating twice for 3 hours at a temperature of 50°C. After extraction, Whatman No. 1 After filtration with filter paper (Whatman co., Ltd., Kent, UK), it was concentrated using a vacuum concentrator (R-114 Rotavapor system, Buchi Labortechnik AG, Flawil, Switzerland), and the concentrate was recovered with distilled water and lyophilized ( LP 20, Ilshinbiobase, Yangju, Korea). After drying, the sample was powdered and stored at -20°C for use in the experiment.

Example 2: Human Skin Fibroblast Culture

Human dermal fibroblasts (HDFs) cells, which are human skin fibroblasts, were provided by the Korea Food Research Institute and used. For HDF cells, Dulbecco's modified Eagle Medium (DMEM, Gibco, USA) medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin solution (Gibco, USA) was used. and incubated at 37° C., 5% CO _{2 conditions.} HDF cells were used for a number of generations between 5-10, and subcultured at intervals of 2-3 days was used for the experiment.

Experimental Example 1: Confirmation of cytoprotective efficacy of the root extract in UV irradiation environment

In order to confirm whether the glycolytic root extract according to the present invention has an effect of protecting cells from UV rays, the cell viability was measured by irradiating UV rays (sUV) after the HDF cells were treated with the glycolytic root extract. As a control group, a group that was not treated with the glycolytic root extract of the present invention was used. The sUV source used in this experiment was purchased from Q-Lab Corporation (Cleveland, OH, USA), and as a result of measurement through a UV meter, the ratio of UVA and UVB generated through the UVA-340 lamp was 94.5% and 5.5%, respectively. It was confirmed that

Specifically, HDF cells were seeded at a ^{concentration of 1Х10 4} cells/well in a 96 well plate and cultured for 24 hours. After culturing, the medium was replaced with serum-free DMEM, and the extract was treated at a concentration of 12.5 μg/mL and cultured for 1 hour. After that, after exposure to sUV of 23 kJ/cm ² intensity for 20 minutes, incubated for 24 hours, the medium supernatant was recovered for analysis. In order to measure the cell viability, the cells were treated with the MTT solution diluted in the recovered medium and reacted for 2 hours. After dissolving the formed formazan in 100 μL of DMSO, the absorbance was measured at 570 nm using a microplate reader.

As a result, as shown in Figure 1, the cell viability of the control group treated only with sUV was reduced to 80% level compared to the group not treated with sUV, whereas in the experimental group treated with the glycolytic root extract, the cell viability increased compared to the control group. appeared, confirming the cytoprotective efficacy of the root extract from ultraviolet rays.

Experimental Example 2: Confirmation of MMP-1 expression inhibition efficacy of the root extract in UV irradiation environment

In order to confirm the effect of the glycolytic root extract according to the present invention on MMP-1 expression under ultraviolet irradiation environment, HDF cells were treated with the glycolytic root extract and irradiated with ultraviolet (sUV), followed by Human MMP-1 ELISA Kit (ab100603, Abcam Inc., UK) was used to measure the MMP-1 expression level. As a control group, a group that was not treated with the glycolytic root extract of the present invention was used.

Specifically, the HDF cells were treated with an extract of the corresponding root ^{and irradiated with sUV of 23 kJ/cm 2} and cultured for 24 hours. Then, 100 μL of standard and medium supernatant were dispensed into each well and reacted at room temperature for 2 hours and 30 minutes, and then washed 4 times with 300 μL of 1X wash solution. After completely removing the solution remaining in each well, 100 μL of 1X biotinylated-MMP1 detection antibody was dispensed and reacted for 1 hour. After the reaction, the wells were washed in the same manner, 100 μL of 1X HRP-streptavidin solution was dispensed, and incubated for 45 minutes at room temperature. After washing each well in the same way as before, 100 μL of TMB One-Step substrate reagent was dispensed, light was blocked, and reacted for 30 minutes. After dispensing 50 μL of stop solution into each well, absorbance was measured at 450 nm using a microplate reader.

As a result, as shown in FIG. 2 , the expression of MMP-1 in the control group treated only with sUV was increased by about 1.7 times compared to the group not treated with sUV, whereas in the experimental group treated with the glycolytic root extract, MMP-1 was also treated in the sUV environment. was inhibited, and MMP-1 expression was significantly decreased compared to the control group, confirming that the extract of the glycolytic root had the effect of inhibiting the UV-induced expression of MMP-1.

Experimental Example 3: Confirmation of procollagen expression protection efficacy of the root extract in UV irradiation environment

In order to confirm the effect of the glycolytic root extract according to the present invention on the expression of pro-collagen under an ultraviolet irradiation environment, HDF cells were treated with the glycolytic root extract and irradiated with ultraviolet (sUV), then Human Pro-Collagen I The amount of procollagen expression was measured using the alpha 1 CatchPoint® SimpleStep ELISA® Kit (ab229389, Abcam Inc., UK). As a control group, a group that was not treated with the glycolytic root extract of the present invention was used.

Specifically, the HDF cells were treated with an extract of the corresponding root ^{and irradiated with sUV of 23 kJ/cm 2} and cultured for 24 hours. Then, 50 μL of standards and medium supernatant were dispensed into each well, and 50 μL of antibody cocktail was dispensed, shaken at 400 rpm, and reacted for 1 hour at room temperature. After the reaction, it was washed 3 times with 350 μL of 1X wash buffer, and 100 μL of CatchPoint HRP solution was dispensed and incubated for 10 minutes while blocking light. Immediately after completion of the reaction, absorbance was measured under the conditions of 530/570/590 (Ex/Cutoff/Em) using a microplate reader.

As a result, as shown in FIG. 3 , the expression of procollagen in the control group treated only with sUV was reduced to 63.6% compared to the group not treated with sUV, whereas in the experimental group treated with the glycolytic root extract, the expression of procollagen even in the sUV treatment environment. This concentration was shown to significantly increase, and it was confirmed that the extract of the corresponding root extract inhibited the decrease in procollagen expression caused by ultraviolet rays and had a procollagen-generating effect.

Experimental Example 4: Confirmation of the skin improvement effect of the glycolytic root extract through animal testing

Statistical processing

The following experimental results were expressed as mean ± standard error of the mean, and statistical processing was performed using GraphPad Prism 7 (GraphPad Software, Inc., SanDiego, CA). Statistical processing was performed using one-way analysis of variance (ANOVA), and the derived values were tested for significance at the level of P <0.05 by Dunnett's Multiple Comparison test (# p <0.05, ## p <0.01 and ### p <0.001 Vs. Normal control, *p <0.05, **p <0.01 and ***p <0.001 Vs. UVB control).

Experimental Example 4-1: Breeding of experimental animals and UV irradiation

The experimental animal used in this example was a hairless mouse (HR-1 hairless mice, male, 6 weeks old), received from Central Laboratory Animal Co., Ltd. (Seoul, Korea) and acclimatized to the breeding room environment for a week, and then randomly assigned to a group So the experiment was carried out.

The experimental group was a normal group (Normal control group; UVB non-treated group), control group (UVB only treated group), glycosylated root extract 100 mg/kg/day administered group (RR-r100), and glycolytic root extract 200 mg/kg/ Day administration group (RR-r 200) and positive control group (Phosphatidyl Serin 50 mg/kg/day administration group (PS 50 group), which helps to maintain skin health from UV-induced skin damage). All materials were administered 5 times a week for 9 weeks, and 0.5% carboxymethyl cellulose (CMC) solution was used as an excipient.

As for the feed, general solid feed (Purina Lab Rodent Chow #38057, Purina Co., Seoul Korea) was fed to all groups so that they were freely ingested with drinking water. The breeding environment was temperature 23±1℃, humidity 50±5%, noise 60 Phone or less, 12-hour light/dark cycle, illuminance 150~300 Lux, and ventilation 10~12 times per hour were maintained.

In addition, for ultraviolet irradiation (Ultraviolet; UVB), put the hairless mouse of this example in a cage for ultraviolet irradiation, and use UV Crosslinker CL-1000S (UVP Inc., CA, USA) to evenly irradiate the dorsal part of the mouse with UVB. and 3 times a week. The light source of the irradiated ultraviolet was using an ultraviolet sunlamp emitting UVB of 254 nm wavelength, 60 mJ/cm ^{2 in the} 1st week, 120 mJ/cm ² in the 2nd week, 180 mJ/cm ^{2 in the} 3rd week, From the 4th week, ultraviolet rays were irradiated with a light quantity of ^{240 mJ/cm 2 .} After inhalation anesthesia of the mice on the day of breeding, blood and skin tissues were collected and used in an experiment for evaluating the efficacy of the material.

Experimental Example 4-2: Weight change and hepatotoxicity evaluation of UV-irradiated mice according to administration of the glycolytic root extract

In the mice prepared by the method of Experimental Example 4-1, the change in body weight according to the administration of the glycolytic root extract was measured once a week at a constant time during the breeding period. In addition, after separating serum from blood for hepatotoxicity evaluation following administration of the glycolytic root extract, AST activity assay kit (MAK055, Sigma aldrich, USA) and ALT activity assay kit (MAK052, Sigma aldrich, USA) were used to AST ( Aspartate Aminotransferase) and ALT (Alanine Aminotransferase) activities were measured.

Specifically, after dispensing 50 μL of serum or standard in a 96-well plate, 100 μL of the reaction mix solution is dispensed into each well and shaken with a horizontal shaker at 37° C. for a certain period of time. reacted while Absorbance was measured at a wavelength of 450 nm (AST) or 570 nm (ALT) using a spectrophotometer (SpectraMax M5, Molecular Devices, CA, USA) at 5 minute intervals in a state in which light was blocked during the reaction. The reaction was terminated when the value was measured to be higher than the measured value of the highest concentration of the standard. After the start of the reaction, the AST and ALT activities were analyzed through the change between the initial measured value (T initial) and the last measured value (T final).

As a result, as shown in FIG. 4 , the normal group of hairless mice, the UVB alone irradiated group, the glycolytic root extract concentration-treated group, and the positive control group did not show any significant change in body weight. In addition, in the serum AST of FIG. 5(a) and the ALT result of FIG. 5(b), there was no statistically significant change according to the administration of the glycolytic root extract.

Experimental Example 4-3: Evaluation of skin moisturizing rate of UV-irradiated mice according to administration of the glycolytic root extract

In the mouse prepared by the method of Experimental Example 4-1, the moisture content was measured using a skin analysis device (WillCam, KL Global, Seoul, Korea) to evaluate the skin moisture content (%) according to the administration of the glycolytic root extract. Measurements were made on the local skin.

As a result of evaluating the skin moisture content of hairless mice, as shown in FIG. 6 , the skin moisture content showed a significant decrease by 24.4% in the UVB-only irradiation group compared to the normal group (100%), whereas 100 mg of the glycolytic root extract Both the /kg and 200 mg/kg administration groups showed a significant increase in the moisturizing rate to a level of 98.9%, confirming the skin moisturizing effect of the K. root extract ( P <0.01).

Experimental Example 4-4: Evaluation of skin thickness and wrinkle generation in UV-irradiated mice according to administration of the glycolytic root extract

In order to evaluate the skin thickness according to the administration of the glycolytic root extract in the mouse prepared by the method of Experimental Example 4-1, hold the part 1cm in width from the center of the back of the hairless mouse to form a double layer, and then use a digital caliper (CD-15APX, Mitutoyo, Japan) was used.

In addition, in order to analyze the skin wrinkle change, a replica kit (Epigem Inc., Seoul, Korea) was used to apply silicone polymer to the skin on the back of the hairless mouse, and then left for a certain period of time to harden, and then a replica was obtained. . Wrinkle area and wrinkle length were measured in the manufactured skin replica using a wrinkle analyzer (Visioline VL650, CK Electronics GmbH, Cologne, Germany).

As a result of evaluating the skin thickness of hairless mice irradiated with UVB according to the administration of the glycolytic root extract, as shown in FIG. 7a , a significant 1.78-fold increase in skin thickness was observed in the UVB-only irradiated group compared to the normal group, whereas the glycolytic root extract was administered In one group, the concentration-significantly decreased skin thickness was observed. In particular, in the group administered with the glycolytic root extract 200 mg/kg, it was confirmed that there was a significant decrease by 22% compared to the group irradiated with UVB alone ( P <0.01). It was confirmed that the increase in thickness was improved.

In addition, as a result of measuring the wrinkle area and wrinkle length of hairless mice irradiated with UVB, as shown in FIGS. 7b and 7c , the wrinkle area and wrinkle length were significantly increased in the UVB-only irradiated group compared to the normal group, and the corresponding muscle In the group administered with the extract, the wrinkle area and wrinkle length were significantly decreased in concentration, and it was confirmed that the skin wrinkles caused by UVB irradiation were improved according to the administration of the glycolytic root extract.

Experimental Example 4-5: Histological evaluation of the skin of UV-irradiated mice according to the administration of the glycolytic root extract

In mice prepared by the method of Experimental Example 4-1, changes in skin epidermal thickness according to the administration of the glycolytic root extract were evaluated through histological analysis of the skin. After fixing the skin tissue cut at the time of autopsy of the mouse with 10% formalin, it was embedded in paraffin to prepare a paraffin block, and sectioned to a thickness of 4 μm to obtain a tissue section. After that, hematoxylin and eosin staining (H&E staining) was performed, and images were obtained with a digital slide scanner (Motic Easyscan one, Motic, Hong Kong). Epidermis thickness of the skin was analyzed using image analysis software (Motic DS Assistant, Hong Kong).

As a result of analyzing the epidermis thickness by performing H&E staining, as shown in FIG. 8a, the skin epidermis thickness increased in the UVB-only group compared to the normal group, and compared to the UVB-only group, the group administered by the corresponding muscle concentration and In the positive control group, the epidermal thickness of the skin was decreased.

As can be seen in the graph of FIG. 8b, in the UVB-only irradiation group, a significant increase in epidermal thickness of about 3.9 times compared to the epidermal thickness of the normal group was observed, and when the glycolytic root extract was administered, it was concentration-dependently compared to the UVB-only irradiation group. It was found that the skin epidermal thickness decreased. A statistically significant result (P <0.01) was confirmed in that the skin epidermal thickness decreased by about 23.3% in the 100 mg/kg group and about 37.2% in the 200 mg/kg group compared to the UVB alone irradiation group.

Experimental Example 4-6: Evaluation of protein expression related to the generation of skin wrinkles in UV-irradiated mice according to the administration of the glycolytic root extract

Protein expression levels of MMP1, MMP3, MMP9, and Pro-COL1A1 through western blot to analyze the protein expression changes related to skin wrinkle generation according to the administration of the glycolytic root extract in mice prepared by the method of Experimental Example 4-1 was confirmed. A certain amount of skin tissue was cut from the hairless mouse back and homogenized by adding a lysis buffer containing a protease inhibitor (sigma aldrich, USA). The tissue lysate obtained by homogenization was quantified with DC protein assay kit (Bio-rad, CA, USA), the same amount of protein was taken from each, and Laemmli sample buffer (Bio-rad, CA, USA) was mixed with SDS- PAGE was performed. When the separation of the protein loaded on the gel was completed, it was transferred to a nitrocellulose membrane, and then the membrane was immersed in a blocking buffer (5% skim milk in TBST) and blocked at room temperature for 1 hour. After the reaction membrane was washed three times with TBST, it was reacted with primary antibodies of factors related to wrinkle formation at 4°C for at least 16 hours. After the reaction, the membrane was washed three times with TBST and reacted with a secondary antibody suitable for each primary antibody at room temperature for 1 hour. After the reaction was completed, the membrane was washed three times with TBST and treated with a chemiluminescence reagent (DoGenBio, Seoul, Korea) for a certain period of time. The expression level was evaluated by developing the band.

In addition, the change was confirmed using the TIMP1 ELISA kit (ab196265, Abcam, UK) to evaluate the expression level of TIMP1. After dispensing 50 μL of tissue homogenate or standard in a 96-well plate, 50 μL of an antibody cocktail was dispensed into each well and reacted for 1 hour at room temperature by shaking using a shaker. After the reaction, each well was washed 3 times with washing buffer, and 100 μL of TMB substrate was dispensed and reacted for a certain period of time. After dispensing 100 μL of stop solution to terminate the reaction, the reaction was shaken for 1 minute in a shaker, and absorbance was measured at a wavelength of 450 nm with a spectrophotometer.

As a result, as shown in FIG. 9A , the expression levels of each of the MMP1, MMP3 and MMP9 proteins were increased in the UVB-only irradiation group compared to the normal group, and the expression level of the positive control group was reduced to a level similar to that of the normal group. In the case of MMP1, the expression level was significantly decreased in the 200 mg/kg group of the glycolytic root extract. In the case of MMP3 and MMP9, the expression levels of the glycolytic root extract decreased in a concentration-dependent manner, and it was confirmed that the expression levels were regulated according to the administration of the glycolytic root extract. In the case of Pro-COL1A1, the expression level was decreased in the UVB alone irradiation group compared to the normal group, the expression level in the positive control group was increased, and the expression level in the group administered with the glycolytic root extract 200 mg/kg was also increased.

In addition, in the TIMP1 expression result of FIG. 9b , the TIMP1 expression level of the UVB-only irradiation group was significantly decreased by about 42% compared to the normal group, and the TIMP1 expression level of the glycolytic root extract-administered group was at both the administration concentration of 100 and 200 mg/kg. It was confirmed that the expression level of TIMP1 was recovered to a level similar to that of the normal group. Therefore, it was confirmed that the glycolytic root extract had the effect of relieving the generation of wrinkles by inhibiting the action of MMPs by restoring the decrease in TIMP1 expression caused by UVB irradiation.

Experimental Example 4-7: Protein expression evaluation related to skin moisturizing of UV-irradiated mice according to the administration of the glycolytic root extract

In order to analyze the protein expression change related to skin moisturizing control according to the administration of the glycolytic root extract in mice prepared by the method of Experimental Example 4-1, HAS1 was performed by western blot in the same manner as in Experimental Example 4-6. , the expression levels of HAS2, HAS3 and Filaggrin proteins were confirmed.

In addition, to measure the expression levels of HYAL1 (Hyaluronidase 1), HYAL2 (Hyaluronidase 2) and hyaluronic acid, Hyaluronidase-1 ELISA kit (MBS9395469, MYBioSource. USA), Hyaluronidase-2 ELISA kit (MBS2883665, MYBioSource. USA) and An ELISA kit (DHYAL0, R&D system, USA) was used. For evaluation, analysis was performed from the skin tissue of hairless mice according to the experimental method provided in each kit.

As a result, as shown in FIG. 10a , the expression level of HAS1 protein was insignificant in the UVB alone irradiation group compared to the normal group, but the expression level of HAS1 increased in the positive control group, and the expression level of the HAS1 protein was significantly increased in both the glycolytic root extract administration group. appear. In the case of HAS2, the expression level was significantly decreased in the UVB alone irradiation group compared to the normal group, and the expression level was increased in a concentration-dependent manner in the glycolytic root extract group. In the case of HAS3, the expression level of HAS3 was increased in the group administered with 200 mg/kg of the corresponding root compared to the group irradiated with UVB alone. In addition, in the case of filaggrin, the difference between the normal group and the UVB alone irradiation group was not significant, but the expression levels in both the glycolytic root extract administration group and the positive control group were significantly increased.

In addition, as shown in the HYAL1 expression change in FIG. 10b , the HYAL1 expression level in the UVB-only group irradiated was significantly increased 1.5-fold compared to the normal group, and the expression of HYAL1 was significant in all the glycolytic root extract-administered groups compared to the UVB-only irradiated group. was reduced to ( p <0.05).

In the HYAL2 analysis result of FIG. 10c, the UVB-only irradiation group showed a tendency to slightly increase the HYAL2 expression level compared to the normal group, but there was no significant difference, and in the case of the glycolytic root extract administered group, 100 and 200 mg/ At the kg concentration, significant reductions were observed by 19% and 25%, respectively ( P <0.05).

In the results of hyaluronic acid (hyaluronan) in FIG. 10d , the expression level of hyaluronic acid in the UVB-only irradiation group was significantly reduced by about 34% compared to the normal group. In the case of the positive control group, the expression level of hyaluronic acid was significantly increased by about 3.7 times compared to the UVB alone irradiation group ( P <0.001). The expression level of hyaluronic acid showed a tendency to increase compared to the group irradiated with UVB alone in both groups administered 100 mg/kg and 200 mg/kg of the Glycolyte root extract, and increased by 1.4 times ( P <0.05) and 2 times ( P <0.001), respectively. .

Therefore, when the glycolytic root extract was administered to mice irradiated with UVB, HAS, Flaggrin, and HYAL protein expression was regulated, and the expression level of hyaluronic acid increased. It was confirmed that there is an effect of improving the

Claims (9)

A food composition for skin improvement comprising an extract of the Korean radish root as an active ingredient. 2. The method of claim 1
The skin improvement may include preventing skin aging; moisturizing the skin; improving or preventing skin wrinkles; inhibiting, improving or preventing skin damage caused by UV rays; or skin protection from UV rays, a food composition. 3. The method of claim 2,
The UV-induced skin damage is any one selected from the group consisting of UV-induced wrinkle generation, reduced skin elasticity, photoaging, and skin keratinization, a food composition. According to claim 1,
The food composition is formulated in the form of pills, beverages, tea bag tea, instant tea, drinks, granules, tablets or capsules, the food composition. A health functional food comprising the food composition of any one of claims 1 to 4. A pharmaceutical composition for preventing or treating skin wrinkles or dry skin, or preventing or inhibiting skin damage caused by ultraviolet rays, comprising an extract of the corresponding root extract as an active ingredient. 7. The method of claim 6,
The UV-induced skin damage is any one selected from the group consisting of UV-induced wrinkle generation, skin elasticity reduction, photoaging, sunburn, erythema, skin inflammation, photoallergy, phototoxicity, photosensitization, and skin keratinization, a pharmaceutical composition . A cosmetic composition for improving skin comprising an extract of the Korean rhizomes as an active ingredient. A quasi-drug composition for skin improvement comprising an extract of the corresponding root extract as an active ingredient.

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