KR20210148122A - 차세대 시퀀싱을 위한 조성물 및 방법 - Google Patents

차세대 시퀀싱을 위한 조성물 및 방법 Download PDF

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KR20210148122A
KR20210148122A KR1020217030220A KR20217030220A KR20210148122A KR 20210148122 A KR20210148122 A KR 20210148122A KR 1020217030220 A KR1020217030220 A KR 1020217030220A KR 20217030220 A KR20217030220 A KR 20217030220A KR 20210148122 A KR20210148122 A KR 20210148122A
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polynucleotide
region
cases
adapter
nucleic acid
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리챠드 간트
시유안 첸
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트위스트 바이오사이언스 코포레이션
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KR1020217030220A 2019-02-25 2020-02-21 차세대 시퀀싱을 위한 조성물 및 방법 Ceased KR20210148122A (ko)

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Application Number Priority Date Filing Date Title
US201962810321P 2019-02-25 2019-02-25
US62/810,321 2019-02-25
US201962914904P 2019-10-14 2019-10-14
US62/914,904 2019-10-14
US201962926336P 2019-10-25 2019-10-25
US62/926,336 2019-10-25
PCT/US2020/019371 WO2020176362A1 (en) 2019-02-25 2020-02-21 Compositions and methods for next generation sequencing

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KR20210148122A true KR20210148122A (ko) 2021-12-07

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US (2) US20210002710A1 (https=)
EP (1) EP3938505A4 (https=)
JP (1) JP2022521766A (https=)
KR (1) KR20210148122A (https=)
CN (1) CN113728100A (https=)
AU (1) AU2020227672A1 (https=)
CA (1) CA3131514A1 (https=)
WO (1) WO2020176362A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE112022005242T5 (de) 2021-11-01 2024-08-29 Hanon Systems Spiralverdichter und Verfahren zum Herstellen einer für einen Spiralverdichter benötigten Exzenterbuchse

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
MX2022013499A (es) 2020-04-27 2023-01-16 Twist Bioscience Corp Bibliotecas de ácidos nucleicos variantes para coronavirus.
EP4204460A4 (en) 2020-08-26 2024-09-25 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS
CN117043171A (zh) 2020-08-28 2023-11-10 特韦斯特生物科学公司 用于合成的装置和方法
CA3194398A1 (en) * 2020-10-05 2022-04-14 Twist Bioscience Corporation Hybridization methods and reagents
EP4229210A4 (en) 2020-10-19 2025-01-08 Twist Bioscience Corporation METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES
US20220135965A1 (en) * 2020-10-26 2022-05-05 Twist Bioscience Corporation Libraries for next generation sequencing
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
US20220282398A1 (en) * 2021-03-05 2022-09-08 Bioo Scientific Corporation Universal blocking oligonucleotides for reduced off-target hybridization in hybridization capture methods
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
MX2023011660A (es) * 2021-03-31 2023-12-11 Illumina Cambridge Ltd Tecnicas de secuenciacion de genoteca de acido nucleico con deteccion de dimero adaptador.
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
WO2023023285A2 (en) 2021-08-19 2023-02-23 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
US12134656B2 (en) 2021-11-18 2024-11-05 Twist Bioscience Corporation Dickkopf-1 variant antibodies and methods of use
US20230323449A1 (en) * 2021-12-17 2023-10-12 Twist Bioscience Corporation Compositions and methods for detection of variants
EP4460516A2 (en) 2022-01-03 2024-11-13 Twist Bioscience Corporation Bispecific sars-cov-2 antibodies and methods of use
KR20240150780A (ko) * 2022-02-18 2024-10-16 애질런트 테크놀로지스, 인크. 표적 핵산 포획 및 바코딩을 위한 시스템 및 방법
EP4594531A1 (en) * 2022-09-29 2025-08-06 Twist Bioscience Corporation Methods and compositions for genomic analysis
US20250122495A1 (en) * 2022-11-02 2025-04-17 Boe Technology Group Co., Ltd. Blockers, Kits and Methods of Use Thereof
EP4627111A2 (en) * 2022-11-07 2025-10-08 Icahn School of Medicine at Mount Sinai Cost-effective long read metagenomic sequencing
JP2026506068A (ja) * 2023-02-17 2026-02-20 ツイスト バイオサイエンス コーポレーション 正規化のための試薬および方法
CN116627972B (zh) * 2023-05-25 2024-03-01 成都融见软件科技有限公司 一种覆盖指标的结构化数据离散存储系统

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2104160T3 (es) * 1992-07-31 1997-10-01 Behringwerke Ag Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos.
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
AU2003233243A1 (en) * 2002-05-06 2003-11-17 Noxxon Pharma Ag Method for amplifying nucleic acids
PT2828218T (pt) * 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
ES2769796T3 (es) * 2012-07-03 2020-06-29 Integrated Dna Tech Inc Oligonucleótidos de bloqueo aumentados en Tm y señuelos para un enriquecimiento de diana mejorado y una selección fuera de diana reducida
US10648103B2 (en) * 2014-10-10 2020-05-12 Invitae Corporation Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions
CA2975855C (en) * 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US10844428B2 (en) * 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
CN108138230B (zh) * 2015-07-21 2023-03-10 夸登特健康公司 用于捕获融合基因的锁核酸
CA3006792A1 (en) * 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
IL269431B2 (en) * 2017-03-23 2025-05-01 Univ Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
US11274344B2 (en) * 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
AU2018260627B2 (en) * 2017-04-23 2024-08-22 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN110770353B (zh) * 2017-04-23 2024-11-26 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
EP3622089B1 (en) * 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
EP3649236A4 (en) * 2017-07-05 2021-04-07 The Regents of The University of California Multiplexed receptor-ligand interaction screens

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE112022005242T5 (de) 2021-11-01 2024-08-29 Hanon Systems Spiralverdichter und Verfahren zum Herstellen einer für einen Spiralverdichter benötigten Exzenterbuchse

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EP3938505A4 (en) 2022-11-30
US20210207197A1 (en) 2021-07-08
CN113728100A (zh) 2021-11-30
AU2020227672A1 (en) 2021-10-07
WO2020176362A1 (en) 2020-09-03
US20210002710A1 (en) 2021-01-07
EP3938505A1 (en) 2022-01-19
CA3131514A1 (en) 2020-09-03

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