AU2020227672A1 - Compositions and methods for next generation sequencing - Google Patents
Compositions and methods for next generation sequencing Download PDFInfo
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- AU2020227672A1 AU2020227672A1 AU2020227672A AU2020227672A AU2020227672A1 AU 2020227672 A1 AU2020227672 A1 AU 2020227672A1 AU 2020227672 A AU2020227672 A AU 2020227672A AU 2020227672 A AU2020227672 A AU 2020227672A AU 2020227672 A1 AU2020227672 A1 AU 2020227672A1
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Families Citing this family (44)
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| SG11201600853UA (en) | 2013-08-05 | 2016-03-30 | Twist Bioscience Corp | De novo synthesized gene libraries |
| US10669304B2 (en) | 2015-02-04 | 2020-06-02 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| EP3350314A4 (en) | 2015-09-18 | 2019-02-06 | Twist Bioscience Corporation | OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF |
| KR102794025B1 (ko) | 2015-09-22 | 2025-04-09 | 트위스트 바이오사이언스 코포레이션 | 핵산 합성을 위한 가요성 기판 |
| EP3500672A4 (en) | 2016-08-22 | 2020-05-20 | Twist Bioscience Corporation | DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES |
| US10417457B2 (en) | 2016-09-21 | 2019-09-17 | Twist Bioscience Corporation | Nucleic acid based data storage |
| EP4556433A3 (en) | 2017-02-22 | 2025-08-06 | Twist Bioscience Corporation | Nucleic acid based data storage |
| WO2018170169A1 (en) | 2017-03-15 | 2018-09-20 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| WO2018231872A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| US11407837B2 (en) | 2017-09-11 | 2022-08-09 | Twist Bioscience Corporation | GPCR binding proteins and synthesis thereof |
| CN111565834B (zh) | 2017-10-20 | 2022-08-26 | 特韦斯特生物科学公司 | 用于多核苷酸合成的加热的纳米孔 |
| KR102804057B1 (ko) | 2018-01-04 | 2025-05-07 | 트위스트 바이오사이언스 코포레이션 | Dna 기반 디지털 정보 저장 |
| CA3100739A1 (en) | 2018-05-18 | 2019-11-21 | Twist Bioscience Corporation | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| WO2020139871A1 (en) | 2018-12-26 | 2020-07-02 | Twist Bioscience Corporation | Highly accurate de novo polynucleotide synthesis |
| CN113766930B (zh) | 2019-02-26 | 2025-07-22 | 特韦斯特生物科学公司 | Glp1受体的变异核酸文库 |
| JP2022522668A (ja) | 2019-02-26 | 2022-04-20 | ツイスト バイオサイエンス コーポレーション | 抗体を最適化するための変異体核酸ライブラリ |
| US11332738B2 (en) | 2019-06-21 | 2022-05-17 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
| EP4034566A4 (en) | 2019-09-23 | 2024-01-24 | Twist Bioscience Corporation | VARIANT NUCLEIC ACID BANKS FOR CRTH2 |
| JP2022548783A (ja) | 2019-09-23 | 2022-11-21 | ツイスト バイオサイエンス コーポレーション | 単一ドメイン抗体のバリアント核酸ライブラリー |
| BR112022011235A2 (pt) | 2019-12-09 | 2022-12-13 | Twist Bioscience Corp | Bibliotecas de variantes de ácido nucleico para receptores de adenosina |
| MX2022013499A (es) | 2020-04-27 | 2023-01-16 | Twist Bioscience Corp | Bibliotecas de ácidos nucleicos variantes para coronavirus. |
| EP4204460A4 (en) | 2020-08-26 | 2024-09-25 | Twist Bioscience Corporation | METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS |
| CN117043171A (zh) | 2020-08-28 | 2023-11-10 | 特韦斯特生物科学公司 | 用于合成的装置和方法 |
| CA3194398A1 (en) * | 2020-10-05 | 2022-04-14 | Twist Bioscience Corporation | Hybridization methods and reagents |
| EP4229210A4 (en) | 2020-10-19 | 2025-01-08 | Twist Bioscience Corporation | METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES |
| US20220135965A1 (en) * | 2020-10-26 | 2022-05-05 | Twist Bioscience Corporation | Libraries for next generation sequencing |
| KR20230147617A (ko) | 2021-01-21 | 2023-10-23 | 트위스트 바이오사이언스 코포레이션 | 아데노신 수용체에 관한 방법 및 조성물 |
| US20220282398A1 (en) * | 2021-03-05 | 2022-09-08 | Bioo Scientific Corporation | Universal blocking oligonucleotides for reduced off-target hybridization in hybridization capture methods |
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| MX2023011660A (es) * | 2021-03-31 | 2023-12-11 | Illumina Cambridge Ltd | Tecnicas de secuenciacion de genoteca de acido nucleico con deteccion de dimero adaptador. |
| WO2022235584A1 (en) | 2021-05-03 | 2022-11-10 | Twist Bioscience Corporation | Variant nucleic acid libraries for glycans |
| US12201857B2 (en) | 2021-06-22 | 2025-01-21 | Twist Bioscience Corporation | Methods and compositions relating to covid antibody epitopes |
| WO2023023285A2 (en) | 2021-08-19 | 2023-02-23 | Twist Bioscience Corporation | Methods and compositions relating to covalently closed nucleic acids |
| KR102871315B1 (ko) | 2021-11-01 | 2025-10-15 | 한온시스템 주식회사 | 스크롤 압축기 및 이에 필요한 편심 부시 제조 방법 |
| US12134656B2 (en) | 2021-11-18 | 2024-11-05 | Twist Bioscience Corporation | Dickkopf-1 variant antibodies and methods of use |
| US20230323449A1 (en) * | 2021-12-17 | 2023-10-12 | Twist Bioscience Corporation | Compositions and methods for detection of variants |
| EP4460516A2 (en) | 2022-01-03 | 2024-11-13 | Twist Bioscience Corporation | Bispecific sars-cov-2 antibodies and methods of use |
| EP4594531A1 (en) * | 2022-09-29 | 2025-08-06 | Twist Bioscience Corporation | Methods and compositions for genomic analysis |
| US20250122495A1 (en) * | 2022-11-02 | 2025-04-17 | Boe Technology Group Co., Ltd. | Blockers, Kits and Methods of Use Thereof |
| EP4627111A2 (en) * | 2022-11-07 | 2025-10-08 | Icahn School of Medicine at Mount Sinai | Cost-effective long read metagenomic sequencing |
| JP2026506068A (ja) * | 2023-02-17 | 2026-02-20 | ツイスト バイオサイエンス コーポレーション | 正規化のための試薬および方法 |
| CN116627972B (zh) * | 2023-05-25 | 2024-03-01 | 成都融见软件科技有限公司 | 一种覆盖指标的结构化数据离散存储系统 |
Family Cites Families (16)
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| AU2003233243A1 (en) * | 2002-05-06 | 2003-11-17 | Noxxon Pharma Ag | Method for amplifying nucleic acids |
| PT2828218T (pt) * | 2012-03-20 | 2020-11-11 | Univ Washington Through Its Center For Commercialization | Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus |
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| US10648103B2 (en) * | 2014-10-10 | 2020-05-12 | Invitae Corporation | Universal blocking oligo system and improved hybridization capture methods for multiplexed capture reactions |
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| US10844428B2 (en) * | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
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| US11274344B2 (en) * | 2017-03-30 | 2022-03-15 | Grail, Inc. | Enhanced ligation in sequencing library preparation |
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- 2020-02-21 US US16/798,275 patent/US20210002710A1/en not_active Abandoned
- 2020-02-21 JP JP2021549708A patent/JP2022521766A/ja active Pending
- 2020-02-21 EP EP20763594.7A patent/EP3938505A4/en not_active Withdrawn
- 2020-02-21 KR KR1020217030220A patent/KR20210148122A/ko not_active Ceased
- 2020-02-21 AU AU2020227672A patent/AU2020227672A1/en not_active Abandoned
- 2020-02-21 CN CN202080031218.7A patent/CN113728100A/zh active Pending
-
2021
- 2021-01-21 US US17/154,906 patent/US20210207197A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4479557A4 (en) * | 2022-02-18 | 2026-02-25 | Agilent Technologies Inc | SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID CAPTURE AND BARCODED |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022521766A (ja) | 2022-04-12 |
| EP3938505A4 (en) | 2022-11-30 |
| US20210207197A1 (en) | 2021-07-08 |
| CN113728100A (zh) | 2021-11-30 |
| WO2020176362A1 (en) | 2020-09-03 |
| US20210002710A1 (en) | 2021-01-07 |
| EP3938505A1 (en) | 2022-01-19 |
| KR20210148122A (ko) | 2021-12-07 |
| CA3131514A1 (en) | 2020-09-03 |
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