KR20210143037A - Fermented black ginseng composition prepared using yeast fermentation broth containing medicinal herbs and minerals and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a fermented black ginseng composition manufactured by using a yeast fermentation broth containing medicinal herbs and minerals and a manufacturing method thereof. According to the present invention, since minerals are added to a mixture of various medicinal herbs and fermenting a herbal mixture by using yeast, the herbal mixture can be fermented more effectively than a case of fermentation by inoculating yeast alone and active ingredients of physiologically active substances contained in the medicinal herbs can be effectively used as food due to the fermentation. In addition, since black ginseng is immersed in a fermentation broth of medicinal herbs and minerals to be fermented and steamed, a large amount of various minerals and active ingredients of the medicinal herbs can be contained in the black ginseng, and an effect of increasing the content of specific useful ginsenoside components in the black ginseng is acquired, thereby being used in various health functional foods. The method comprises a medicinal herb mixture preparation step, a mineral yeast fermentation broth preparation step, a fermentation step, a steaming step, a drying step, and a fermented black ginseng manufacturing step.

Description

Fermented black ginseng composition prepared using yeast fermentation broth containing medicinal herbs and minerals and manufacturing method thereof

The present invention relates to a fermented black ginseng composition prepared by using a herbal mineral yeast fermentation broth and a method for producing the same.

Ginseng is a representative nourishing and tonic agent that has been used for a long time, and many pharmacological studies have been conducted on its ingredients. Ginseng exhibits various effects such as improvement of hyperlipidemia, enhancement of liver function, anti-stress effect, immune enhancement, improvement of brain function, and anti-cancer effect, and plays a role in maintaining homeostasis of the human body. In particular, ginseng saponin has a unique chemical structure that is different from saponin found in other plants, and has a unique pharmacological effect.

These ginseng saponins are generally known to exist in fresh ginseng, but some specific ginseng saponins are known to exist only in processed ginseng. Recently, many studies have been conducted to further increase the content of ginseng saponins, which are only present in these processed ginseng.

Ginsenosides contained in ginseng can be largely divided into panaxadiol (PD) and excitatory panaxatriol (PT), and the effects of major ginsenosides are summarized in Table 1 below.

In particular, black ginseng refers to ginseng in which ginseng has turned black after several times of steaming and drying. In addition, ginseng saponins that are basically present are not lost through several times of proliferation and drying, and ginseng that exists only in processed ginseng. It is known that the content of saponin is increased, so research on a method for producing such black ginseng more effectively is being actively conducted.

That is, the content of the active ingredient (saponin) of black ginseng varies greatly depending on the conditions under which steaming and drying of black ginseng is repeated. is being done

Poria cocas is a white or grayish-white mycelium parasitic in the soil of a pine tree stump. When taken, the qi and blood are common, giving the hair a shine, alleviating or healing hair loss, and has excellent nourishing and tonic properties. It is known to have the effect of lightening and brightening the skin.

Glycyrrhiza uralensis (Glycyrrhiza uralensis) has been used as a basic useful medicine indispensable in oriental medicine. Licorice not only makes the stomach of the human body healthy, but it also treats cold sores in the intestines and intestines, strengthens muscles and bones, as well as treats various boils and bruises, detoxifies the poison of all kinds of medicinal herbs, helps the lungs and helps with cooling and fever. It is a medicinal herb with great detoxification effect. It relieves acute symptoms such as cramps or pain, eliminates heat poisoning, and relieves inflammation, so it is effective when used in sore throats or boils. In addition, glycyrrhizin, one of the main components of licorice, is a component that imparts a unique sweetness to licorice.

Rehmanniae radix preparata is warm in nature, sweet in taste, slightly bitter, and non-toxic. It is used for irregular menstruation, weak constitution, stunted growth in children, dementia, premature ejaculation, and erectile dysfunction. It improves blood circulation and improves peripheral microcirculation in various chronic diseases. Inhibition of the immune system, the effect of increasing immunity, anticancer action, increase in fibrinolytic system activity, reduction of polybrene-induced red blood cell aggregation, and increase in red blood cell deformability and ATP content have been reported.

Paeoniae radix alba is bitter in taste and slightly cold in nature. It enriches blood, stops pain and sweat, stops hemostasis, eliminates liver fire, and makes urine come out well. One of the active ingredients, pheniflorin, has sedative, analgesic, antispasmodic, antipyretic, anti-inflammatory, anti-ulcer, blood pressure lowering, and coronary vasodilation effects. It exhibits analgesic action, antispasmodic action, anti-inflammatory action, and hemostatic action. Peony also exhibits antibacterial action. Hemorrhagic disease, abdominal pain due to blood clots, abdominal pain due to gastrointestinal spasm, cramping pain in arms and legs, neuralgia, rheumatoid arthritis, menstrual pain, chest pain, headache and dizziness due to liver cirrhosis, nervous breakdown, frequent menstruation, irregular uterine bleeding , For spontaneous sweating, it is used for cold sweat, dysentery, dysentery, and pimples.

Cinnamomi cortex is the trunk and branch bark of Cinnamomum loureirii or Cinnamomum cassia , and the main components are cinnamicaldehyde, camphene, cineol, linalool (Cineol), Linalool) and Eugenol are known to have soothing and antibacterial effects.

Baekchul ( Atractylodis rhizoma ) is known as attractylone (Atractylone), attractylenolide (Atractylenolide) I, Ⅱ, Ⅲ, beta-eudesmol (ß-Eudesmol), hynesol (Hynesol), etc. as a major component. It is a root plant of the Asteraceae family with a bitter and sweet taste. It swells the spleen and has effects such as gastric, diuretic, nourishing, and sedative.

Cnidium officinale contains a large amount of aromatic essential oil, so it smells good. The main ingredients include cnidilide, neocnidilide, ligustilide, and the like. The young shoots are eaten as a vegetable, and the rhizomes are dried and used as medicine. It has a mild medicinal property and a sour taste, and when it enters the body, it has an analgesic effect that stimulates blood circulation and relieves pain. Therefore, it is often applied to headaches. For women, it eliminates menstrual irregularities, menstrual pain, postpartum abdominal pain and dizziness. In addition, it activates the function of the liver, and when it is taken for anemia, it is effective for hematopoiesis. It is also improving symptoms of pain in the heart area due to insufficiency of contractility of the heart muscle. Those with weak back and leg bones and frequent muscle cramps can take this symptom and improve it.

Angelica gigas is a plant root that has a bitter, sweet or spicy taste, and its main ingredients are coumarin derivatives such as decursin, decursinol, and nodakenin, alpha-pinene. (α-Pinene), limonene (Limonene), beta-eudesmol (β-Eudesmol), elemol (Elemol), imperatorin (Imperatorin) and the like. It is effective for various female diseases, menstrual irregularity, menstrual pain, anemia, underdevelopment of the uterus, lack of blood after childbirth, and menopausal disorders.

Astragalus membranaceus is mainly used for diuresis, tonicity, blood pressure lowering, blood sugar lowering, immunity enhancement, antitumor, antiviral, etc. Substances such as formononetin), triterpenoide glycoside, and beta-sitosterol, as well as saponins, astragaloside and sugar are known as main components.

For example, in the prior art related to the enhancement of the efficacy of ginseng, red ginseng or black ginseng, Korean Patent Publication No. 10-0425022 discloses one selected from omija, hawthorn, cornflower, quince, plum, citron, tangja, apple, pomegranate and orange. Or, a ginsenoside (Rg3+Rg5)/(Rb1+Rb2+Rc+Rd) ratio is enhanced by steaming two or more kinds of herbal medicines with ginseng, but the patent discloses a processed ginseng extract with enhanced ginsenoside Rg3 and An increase in the content of Rg5 was confirmed, and there is only a description that the vasodilatory action was enhanced.

In addition, Korean Patent Publication No. 10-1343819 discloses a red ginseng concentrate composition in combination with vitamins and minerals while using the efficacy of various types of ginsenosides contained in red ginseng, but the patent is a fermentation using yeast bacteria. It is different from the manufacturing method of the present invention in that vitamins and minerals are simply added to the red ginseng concentrate powder without a process.

In addition, Korean Patent Registration No. 10-1667284 discloses a cosmetic composition containing a fermented extract of black ginseng and mixed herbal medicine as an active ingredient. However, the above patent is different from the manufacturing method of the present invention, which undergoes a multi-step yeast fermentation process, since extracts of black ginseng, goji berry, angelica, sorghum and bokbunja are fermented with mushroom mycelium without any additional additives.

After several years of research for the purpose of reinforcing minerals and effective physiologically active ingredients not found in black ginseng, and enhancing the specific ginsenoside content in black ginseng, the present inventors selected a mixed herbal medicine of baekbokryeong, licorice, sukjihwang, white peony and broiler, and mixed these After fermenting the herbal medicine with yeast bacteria containing minerals, black ginseng was added thereto and subjected to additional fermentation and steaming processes to complete the object of the present invention.

Korean Patent No. 0425022, processed ginseng extract and pharmaceutical composition containing the same, registered on March 17, 2004. Korean Patent No. 1343819, red ginseng concentrate powder containing 11 kinds of ginsenosides as active ingredients, anti-stress, anti-hyperlipidemia and anti-obesity composition and health functional food composed of vitamins and minerals, December 16, 2013 Enrollment. Korean Patent No. 1667284, cosmetic composition containing fermented extract of black ginseng and mixed herbal medicine, registered on October 12, 2016.

It is an object of the present invention to provide a method for producing a fermented black ginseng composition prepared by using a herbal mineral yeast fermentation broth.

In addition, another object of the present invention is to provide a fermented black ginseng composition prepared using a herbal mineral yeast fermentation broth and various uses thereof.

The method for producing the fermented black ginseng composition of the present invention,

pulverizing and sterilizing the washed herbal materials to prepare a herbal mixture (step 1);

Adding 0.01 to 2 wt% of minerals and 0.01 to 10 wt% yeast to the total weight of the herbal mixture, and fermenting at 25 to 40 °C for 24 to 48 hours to prepare a herbal mineral yeast fermentation broth (step 2);

immersing 5-100 parts by weight of black ginseng in 100 parts by weight of the herbal mineral yeast fermentation broth of the second step and fermenting it at 25-40 ℃ for 24-48 hours (step 3);

Steaming the fermentation mixture of the third step at 75-80° C. for 6-8 hours (step 4);

Putting the fermentation mixture of the fourth step in a drying room with elvan stone and loess and drying it for 24 to 72 hours by applying hot air at 25 to 40 ° C (step 5);

Putting a mixture of the dried herbal medicine and black ginseng of the fifth step into the tank, and adding an aqueous solution of alcohol 5 to 20 times the weight of the herbal medicine and black ginseng (step 6); and

Heating the tank with a heater so that the internal temperature is maintained at 70-95° C., applying ultrasonic vibration and fine bubbles to the aqueous solution of black ginseng and alcohol to extract it, and then separating and concentrating the extract to prepare a fermented black ginseng composition (Step 7) ; may be included.

The herbal medicine of the first step is included by mixing baekbokryeong, licorice, sukjihwang, ear pea and broiler in a weight ratio of 1-3: 1-3: 1-3: 1-3: 1-3.

In addition, the effect of the present invention is further increased by selectively adding and mixing one or more of ginseng, baekchul, cheongung, angelica and hwanggi in a weight ratio of 1 to 3 to the herbal medicine mixture of baekbokryeong, licorice, sukjihwang, peony and broiler in the first step. can do it

The herbal material of the first step may be washed with a water bath immersion method and a rotary spray washing method, and then dehydrated or dried. In addition, the washed herbal material can be coarsely crushed or crushed, respectively, to a size of 120 to 180 mesh using a grinder. In addition, the crushed herbal material can be transferred to a sterilization tank, steam sterilized at 90-120° C. for 30 minutes to 2 hours, cooled to room temperature, and then transferred to an incubator.

The mineral of the second step may consist of one or more sources from the group consisting of calcium, phosphorus, potassium, iron, sulfur, chlorine, sodium, copper, zinc, manganese, magnesium, selenium, iodine, molybdenum, cobalt, and chromium, Preferably, it may consist of a source of calcium, iron, copper, zinc, manganese, magnesium and selenium.

Furthermore, water-soluble seaweed calcium, iron lactate, copper sulfate, zinc sulfate, manganese sulfate, magnesium sulfate, and sodium selenite are used as sources of calcium, iron, copper, zinc, manganese, magnesium and selenium, which are preferred minerals in the second step. It consists of creating

The yeast of the second step may be at least one selected from the group including Saccharomyces cerevisiae , Saccharomyces ellipsoidus, and fermentation using conventional yeast fermented under conditions.

The aqueous solution of alcohol in the sixth step is preferably 50 to 95% by weight of alcohol, and more preferably 60 to 80% by weight of alcohol.

The herbal medicine and black ginseng mixture of the seventh step was put in a fabric bag and put into water to facilitate the separation of the residue after the extraction operation.

By the method described above, the present invention provides a fermented black ginseng composition and various foods such as health functional food, nutritional supplement food, functional food, health food, and tea food containing the fermented black ginseng composition.

According to the manufacturing method of the present invention, by adding minerals to a mixture of various herbal medicines and fermenting them using yeast, herbal medicines and black ginseng can be fermented more effectively than when fermented by inoculating yeast alone. The active ingredients of the physiologically active substances contained in it can be effectively used as food. In addition, by immersing and fermenting black ginseng in the herbal mineral yeast fermentation broth, a large amount of various mineral components can be contained, and the active ingredients of the herbal medicine are contained in the black ginseng.

In addition, during the dry fermentation process, the dried surface of black ginseng acts as a shielding film, thereby preventing the volatilization of useful volatile components to a large extent and increasing the content of specific ginsenoside components with strong physiological activity in black ginseng. can be obtained In particular, the contents of ginsenosides Rg2, Rd, F4, Rg3, Rk1, and Rg5 were significantly increased compared to that of black ginseng subjected to general fermentation.

The main pharmacological actions of ginsenosides increased in fermented black ginseng prepared by the method of the present invention are shown in Table 2 below.

In addition, the fermented black ginseng composition prepared using the herbal mineral yeast fermented liquid of the present invention can be consumed without objection by consumers by adding the unique taste and flavor of herbal medicines instead of weakening the strong flavor of black ginseng.

The fermented black ginseng composition prepared in the present invention has no particular limitation on intake, and does not exhibit toxicity, like general black ginseng.

Therefore, the present invention may provide the fermented black ginseng composition in the form of a food composition, and the food composition may be in the form of a nutritional supplement, health food, or the like. In addition, by adding the fermented black ginseng composition of the present invention to food, it is possible to easily ingest the active ingredient of black ginseng, enrich the taste and flavor of food, and facilitate digestion and absorption.

1 is a flow chart sequentially showing the manufacturing process of a fermented black ginseng composition prepared by using the herbal mineral yeast fermentation broth of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.

<Example 1. Preparation of fermented black ginseng composition using herbal mineral yeast fermentation broth>

After mixing the washed baekbokryeong, licorice, succulent yellow, white peony, and broiler in a weight ratio of 1: 1: 1: 1: 1, use a grinder to coarsely pulverize or crush each into a size of 120 to 180 mesh to make herbal medicines Prepare the mixture. Then, it is transferred to a sterilization tank, steam sterilized at 90~120°C for 30 minutes to 2 hours, cooled to room temperature, and then transferred to an incubator.

10 g of minerals (calcium, iron, copper, zinc, manganese, magnesium and selenium as a source of water-soluble seaweed calcium (containing 12.1% of calcium), iron lactate, copper sulfate, zinc sulfate, manganese sulfate, magnesium sulfate, Sodium selenite) was added in the same equivalence ratio based on the mineral. In addition, 50 g of Saccharomyces cerevisiae yeast is added and mixed homogeneously, and then fermented for 36 hours at 37° C. to prepare a fermented herbal mineral yeast.

200 g of black ginseng (commercially purchased) is immersed in the prepared herbal medicinal mineral yeast fermentation broth, fermented for 36 hours at 37° C., and then steamed at 75° C. for 7 hours.

The fermented and steamed herbal black ginseng fermentation mixture is placed in a drying room with elvan stone and loess, and is dried for 48 hours by applying hot air at 40°C.

A mixture of dried herbal medicine and black ginseng is put into the tank, and 10L of 70 wt% alcohol is added.

The tank was heated with a heater so that the internal temperature was maintained at 80-85° C., and ultrasonic vibration and microbubbles were applied to the mixture of herbal medicines and black ginseng and the aqueous alcohol solution, extracted and concentrated for 8 hours to prepare about 150 g of a fermented black ginseng composition. .

<Comparative Example 1. Preparation of fermented black ginseng composition using fermented herbal medicinal mineral yeast>

The same procedure as in Example 1, but without mixing minerals (calcium, iron, copper, zinc, manganese, magnesium and selenium source) 50 g of Saccharomyces cerevisiae using yeast fermented herbal medicine yeast A processed herbal medicine black ginseng composition was prepared.

<Example 2. Analysis of ginsenoside and mineral content of fermented black ginseng composition of the present invention>

Analysis of the content of ginsenosides was confirmed under the following analysis conditions by HPLC analysis methods commonly used in the art.

The mineral content was analyzed using an inductively coupled plasma mass spectrometer (ICP/MS: Varian820.Australia). In order to optimize the interference effect of the interfering material in the sample, the CRI (collision reaction interface) system was applied and analyzed, and the analysis conditions were confirmed as follows.

The contents of ginsenosides and minerals according to yeast fermentation or mineral yeast fermentation of the fermented black ginseng composition prepared according to the present invention were analyzed, and the results are shown in Tables 3 and 4 below.

As shown in the ginsenoside content analysis results in Table 3, the fermented black ginseng composition using the mineral yeast fermentation of Example 1 of the present invention has Rg2, Rd, F4 5 times more than the fermented black ginseng composition using the yeast fermentation without the mineral of Comparative Example 1. An excellent fermented black ginseng composition was obtained in which the physiological activities of Rg2, Rd, and F4 ginsenosides (platelet aggregation inhibitory action, plasmin activation action, whitening effect, immunomodulatory aid) were further enhanced due to the increased ginsenoside content.

From the mineral content analysis results in Table 4, the fermented black ginseng composition using mineral yeast fermentation of Example 1 of the present invention obtained an excellent fermented black ginseng composition with enhanced minerals than the fermented black ginseng composition using only yeast fermentation without the minerals of Comparative Example 1.

As can be seen from Tables 3 and 4 above, by adding minerals to the mixture of various herbal medicines of the present invention and fermentation using yeast, the specific ginseno contained in herbal medicines and black ginseng was compared to the case of fermentation by inoculating yeast alone. It can be effectively used as a food fortified with active ingredients of side bioactive substances. In addition, by immersing and fermenting black ginseng in the herbal mineral yeast fermentation broth, a large amount of various mineral components may be contained, and the active ingredients of the herbal medicine may be contained in the black ginseng.

In addition, through an additional special drying process, the dried surface of black ginseng acts as a shielding film to prevent volatilization of useful volatile components to a large extent, and the content of specific ginsenoside components with strong physiological activity in black ginseng increases. effect can be obtained. In particular, the contents of ginsenosides Rg2, Rd, and F4 were significantly increased compared to that of black ginseng subjected to general fermentation, and copper, zinc and manganese components among the 7 minerals were significantly increased.

Through this, the nutritional component of the composition changes depending on whether or not minerals are added in the manufacturing process of the present invention. .

<Experimental Example 1. Platelet aggregation inhibitory activity of fermented black ginseng composition>

Platelets were human concentrated platelets, which were washed once with washing buffer (138 mM NaCl, 2.7 mM KCl, 12 mM NaHCO ₃ , 0.36 _{mM NaH 2} PO ₄ , 5.5 mM Glucose, 1 mM EDTA, pH 6.5). Then, resuspended in suspending buffer (138mM NaCl, 2.7mM KCl, 12mM NaHCO ₃ , 0.36mM NaH ₂ PO ₄ , 5.5mM Glucose, 0.49mM MgCl ₂ , 0.25% gelatin, pH 7.4), and centrifuged at 3,000 rpm for 10 minutes. After separation, it was resuspended in suspending buffer, and the platelet count was adjusted to be ^{4x10 9 /ml.} Then, 2.5 μl collagen was added to 1 ml suspension and reacted for 5 minutes, and platelet aggregation was measured at 37° C. using a whole-blood aggregometer (Chrono-log, USA).

The fermented black ginseng composition of Example 1 and the human platelet aggregation inhibitory activity of Comparative Example 1 were evaluated, and the results are shown in Table 5.

As shown in Table 5, first, aspirin showed strong inhibition of platelet aggregation in a concentration-dependent manner of 37.6% at a concentration of 0.5 mg/ml, thereby confirming the evidence for its use as an antithrombotic agent in clinical practice. On the other hand, the fermented black ginseng composition of Example 1 showed 41.2% human platelet aggregation ability at a concentration of 5 mg/ml, which was significantly superior to platelet aggregation inhibitory activity at a concentration of 5 mg/ml of Comparative Example 1 of 60.7% can be seen to have Therefore, it was confirmed that the fermented black ginseng composition of the present invention exhibits excellent platelet aggregation inhibitory activity.

<Experimental Example 2. Immunity-increasing activity of herbal fermented mineral fermented black ginseng composition>

2-1. Experiments on lymphocyte proliferative activity

A spleen was removed from a 5-week-old Balb/c mouse, the number of splenocytes ^{was adjusted to a density of 2×10 5} /100 μl, and the composition according to the present invention was added to each well at 10 mg/ml. , 1 mg/ml, and 0.1 mg/ml were added.

As a positive control, the final concentrations of Concavanalin-A (hereinafter abbreviated as Con-A) and lipopolysaccharide (hereinafter abbreviated as LPS), which are mitotic substances of T-cells and B-cells, were respectively It was adjusted to be 0.5 μg/ml and 5 μg/ml and treated in each well, and the experimental group and the control group were _{cultured for 3 days at 37° C. and 5% CO 2} conditions, and the proliferation activity of lymphocytes by the sample was investigated.

The proliferative activity of lymphocytes was performed by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] method, and the MTT method was performed with the MTT reagent adjusted to a concentration of 5 mg/ml. was added to each well, incubated for 6 hours, and the amount of insoluble dark blue formazan produced by reacting with MTT reagent and mitochondria dehydrogenase produced by living cells was measured at 570 nm. By measuring the absorbance, the proliferative activity of lymphocytes was measured.

As shown in Table 6, the results of this Experimental Example confirmed that the composition according to the present invention exhibited a higher proliferative activity of splenocytes compared to the sample untreated control group, and had an activity of non-specifically stimulating splenocytes directly.

2-2. Experiments on cytokine induction

The spleen was removed from a 5-week-old Balb/c mouse, the number of splenocytes ^{was adjusted to a density of 2×10 6} /500 μl, and put into each well, and the composition of the present invention was added to each well at 10 mg/ml. , 1 mg/ml, and 0.1 mg/ml were added and co-cultured for 24 hours.

In addition, as a positive control, the final concentration of Con-A was adjusted to 0.5 μg/ml, treated in wells, and cultured for 24 hours. IL-2, IL-4 and IL-10) were measured using each cytokine kit.

As shown in Table 7, the results of this test confirmed that there was an immunostimulatory effect inducing the immune-related cytokines of IL-2, IL-4 and IL10 by directly activating the pancreatic cells of the mouse.

In the present invention, the degree of cytokine induction was different depending on the type of cytokine and the concentration of the composition solution, but it was confirmed that there was activity as a cytokine inducer in a concentration-dependent manner.

The fermented black ginseng composition prepared in the present invention has no particular limitation on intake, and does not exhibit toxicity, like general black ginseng.

In addition, the fermented black ginseng composition using the herbal mineral yeast fermented liquid of the present invention can be consumed without objection by consumers by adding the unique taste and flavor of herbal medicines instead of weakening the strong aroma of black ginseng.

Therefore, the present invention may provide the fermented black ginseng composition in the form of a food composition, and the food composition may be in the form of a nutritional supplement, health food, or the like. By adding the fermented black ginseng composition of the present invention to food, it is possible to easily ingest the active ingredients of black ginseng, enrich the taste and flavor of food, and facilitate digestion and absorption.

<Formulation Example 1. Preparation of health food containing fermented black ginseng composition>

1-1. Manufacturing of health food

The fermented black ginseng composition prepared in Example 1 41 kg, brewer's yeast 20 kg, soy protein isolate 18.4 kg, polydextrose 10 kg, indigestible dextrin 8 kg, vitamin B1 hydrochloride 0.2 kg, vitamin B2 0.1 kg, vitamin B6 hydrochloride 0.1 kg, vitamin C 0.5 kg , 0.5 kg of vitamin E, 1 kg of seaweed calcium, and 0.2 kg of zinc oxide were mixed and processed to prepare a health food.

1-2. Manufacturing of functional foods

Functional food was prepared by mixing and processing 81 kg of the fermented black ginseng composition prepared in Example 1, 1 kg of coenzyme Q10, 2 kg of grape seed extract powder, 2 kg of vitamin C, 2 kg of vitamin E, 10 kg of erythritol, and 2 kg of malic acid.

1-3. Manufacture of nutritional supplements

70 kg of fermented black ginseng composition prepared in Example 1, 26.49 kg of soy protein isolate, 1 kg of seaweed calcium, 0.75 kg of magnesium oxide, 0.02 kg of zinc oxide, 0.2 kg of vitamin B1 nitrate, 0.12 kg of pyridoxine hydrochloride, 0.12 kg of nicotinic acid amide, 0.8 kg of vitamin C kg and 0.5 kg of vitamin E were mixed and processed to prepare a nutritional supplement.

1-4. Manufacture of beverages

A tea food was prepared by mixing and processing 14 kg of the fermented black ginseng composition prepared in Example 1, 12 kg of mate extract, 10 kg of green tea extract powder, 10 kg of prune juice powder, 10 kg of inulin, 20 kg of erythritol, and 24 kg of lactose.

Claims (10)

Pulverizing and sterilizing herbal medicines composed of baekbokryeong, licorice, sukjihwang, ear pea and broiler chicken to prepare a herbal mixture (step 1);
preparing a fermented herbal mineral yeast broth by adding and fermenting 0.01 to 2 wt% of minerals and 0.01 to 10 wt% yeast based on the total weight of the herbal mixture (step 2);
immersing and fermenting 5 to 100 parts by weight of black ginseng based on 100 parts by weight of the herbal mineral yeast fermentation broth of the second step (step 3);
steaming the fermentation mixture of the third step (step 4);
Putting the fermentation mixture of the fourth step in a drying chamber and drying it by applying hot air (step 5);
Putting the mixture of the dried herbal medicine and black ginseng of step 5 into the tank, and adding an aqueous alcohol solution (step 6); and
Preparing a fermented black ginseng composition by extracting the mixture while heating the sixth step, and then separating and concentrating the extract (step 7);
A method for producing a fermented black ginseng composition, characterized in that it consists of. According to claim 1,
The herbal medicine of the first step is fermented black ginseng composition, characterized in that the fermented black ginseng composition is mixed in a weight ratio of 1-3: 1-3: 1-3: 1-3: 1-3, respectively. Way. 3. The method of claim 2,
The herbal medicine of step 1 is obtained by selectively adding at least one of ginseng, baekchul, cheongung, angelica and astragalus in a weight ratio of 1-3: 1-3: 1-3: 1-3: 1-3, respectively. Method for producing a fermented black ginseng composition, characterized in that mixing. According to claim 1,
The mineral of the second step is calcium, phosphorus, potassium, iron, sulfur, chlorine, sodium, copper, zinc, manganese, magnesium, selenium, iodine, molybdenum, cobalt, fermentation, characterized in that at least one mineral selected from chromium A method for producing a black ginseng composition. According to claim 1,
The yeast of the second step is Saccharomyces cerevisiae ( Saccharomyces cerevisiae ), Saccharomyces ellipsoidus ( Saccharomyces ellipsoidus ) Method of producing a fermented black ginseng composition, characterized in that at least one yeast selected from the group consisting of. A fermented black ginseng composition prepared by the method of any one of claims 1 to 5. 7. The method of claim 6,
The composition is a fermented black ginseng composition, characterized in that the ginsenoside Rg2, Rd and F4 content is increased 5 times or more. A health functional food comprising the fermented black ginseng composition of claim 6. A nutritional supplement comprising the fermented black ginseng composition of claim 6. A tea food comprising the fermented black ginseng composition of claim 6.

Images