KR20210140973A - Cosmetics composition containing subtilisin stabilized with non-aqueous emulsion - Google Patents

Abstract

The present invention relates to a cosmetic composition for exfoliating dead skin cells comprising subtilisin. The present invention relates to the cosmetic composition for exfoliating subtilisin, which stabilizes subtilisin in a non-aqueous formulation, and the odor caused by subtilisin decomposition is significantly reduced.

Description

COSMETICS COMPOSITION CONTAINING SUBTILISIN STABILIZED WITH NON-AQUEOUS EMULSION}

The present invention relates to a cosmetic composition for exfoliation comprising subtilisin.

It is desirable that the dead or peeled cells of the epidermis caused by the body's active metabolism naturally fall off after 20 days. However, as the skin ages, wrinkles are formed due to a decrease in water content, a decrease in thickness, and loss of elasticity, and the separation of the stratum corneum is delayed than normal, and the stratum corneum becomes thick and loses vitality. In addition, thick dead skin cells produced by drying and secretion of the epidermal sebaceous glands are difficult to remove and roughen the skin, causing skin aging.

Therefore, in order to create a healthy skin texture, it is necessary to remove dead skin cells that have not fallen off. If you artificially thin the stratum corneum that has not fallen off, the activity or regeneration of living cells is increased to make the skin soft, and the sebum secretion is returned to normal to suppress the occurrence of acne and remove wrinkles. A lot of research is going on.

In order to remove these unnecessary dead skin cells, a physical technique of rubbing with external pressure or a chemical technique of decomposing keratin components are used. In particular, chemical exfoliation technology shows effects such as skin softening, blemish removal, and improvement of fine lines. As materials used to exfoliate, alpha-hydroxy acid (hereinafter, AHA) and beta-hydroxy acid (hereinafter, hereinafter, BHA) have been mainly used. AHA and BHA have an excellent exfoliating effect, but have a problem in being universally used as a cosmetic composition because of their low molecular weight, which is absorbed deeply into the skin and causes irritation.

Recently, a technique using proteolytic enzymes as an active ingredient for exfoliation has been proposed. Subtilisin is an enzyme component obtained from Bacillus subtilis , increases cell detachment and regeneration, and is a naturally derived plant biologically active (PHYTOBIOACTIVE) substance, which may cause less skin irritation. However, there is a problem in that it is easily decomposed at room temperature and is difficult to apply as a cosmetic composition.

Accordingly, the present inventors have developed a cosmetic composition having excellent exfoliating effect by stabilizing subtilisin, which is unstable at room temperature, in the formulation.

Accordingly, it is an object of the present invention to provide a cosmetic composition for exfoliating dead skin cells comprising subtilisin.

The present invention relates to a cosmetic composition for exfoliation comprising subtilisin.

Hereinafter, the present invention will be described in more detail.

An example of the present invention relates to a cosmetic composition for exfoliating dead skin cells comprising subtilisin.

In the present invention, subtilisin is 0.1 to 2.0% by weight, 0.1 to 1.9% by weight, 0.1 to 1.8% by weight, 0.1 to 1.7% by weight, 0.1 to 1.6% by weight, 0.1 to 1.5% by weight, 0.1 to 1.5% by weight relative to the total weight of the cosmetic composition 1.4 wt%, 0.1 to 1.3 wt%, 0.1 to 1.2 wt%, 0.1 to 1.1 wt%, for example, 0.1 to 1.0 wt% may be included. When included within the above range, it helps to promote cell regeneration, so that the skin surface becomes much smoother and wrinkles and other skin defects are reduced, thereby having an excellent effect on skin regeneration.

In the present invention, the cosmetic composition for exfoliation may further include at least one selected from the group consisting of a moisturizer, a thickener, an oil, an oil-absorbing powder, and an emulsifier, for example, a moisturizer, a thickener, an oil, It may further include an oil-absorbing powder and an emulsifier.

In the present invention, the moisturizing agent may be one or more selected from the group consisting of 1,3-butylene glycol, glycerin, diglycerin, glucose and propylene glycol, for example, may be glycerin, but is limited thereto no.

In the present invention, the moisturizing agent is 1.0 to 90.0% by weight, 10.0 to 90.0% by weight, 20.0 to 90.0% by weight, 30.0 to 90.0% by weight, 40.0 to 90.0% by weight, for example, 50 to 90% by weight relative to the total weight of the cosmetic composition may be included. When included within the above range, it helps to maintain skin moisture and has an excellent effect in protecting the skin through moisturizing action.

In the present invention, the thickener may be at least one selected from the group consisting of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, sodium acrylate copolymer, and polyacrylate crosspolymer-6, for example For example, it may be a sodium acrylate copolymer, but is not limited thereto.

In the present invention, the thickener is 0.1 to 5.0% by weight, 0.1 to 4.5% by weight, 0.1 to 4.0% by weight, 0.1 to 3.5% by weight, 0.1 to 3.0% by weight, 0.1 to 2.5% by weight, 0.1 to the total weight of the cosmetic composition to 2.0 wt%, 0.1 to 1.5 wt%, 0.2 to 5.0 wt%, 0.2 to 4.5 wt%, 0.2 to 4.0 wt%, 0.2 to 3.5 wt%, 0.2 to 3.0 wt%, 0.2 to 2.5 wt%, 0.2 to 2.0 wt%, 0.2-1.5 wt%, 0.3-5.0 wt%, 0.3-4.5 wt%, 0.3-4.0 wt%, 0.3-3.5 wt%, 0.3-3.0 wt%, 0.3-2.5 wt%, 0.3-2.0 wt% , for example, 0.3 to 1.5 wt% may be included. When included within the above range, the cosmetic viscosity is increased, so that pickup properties and feeling of use are improved, and there is an excellent effect in preventing loss of active substances.

In the present invention, the oil may be at least one selected from the group consisting of Queensland nut oil, sweet almond oil, squalane and argan kernel oil, but is not limited thereto.

In the present invention, the oil is 1.0 to 50.0% by weight, 1.0 to 40.0% by weight, 1.0 to 30.0% by weight, 1.0 to 20.0% by weight, 5.0 to 50.0% by weight, 5.0 to 40.0% by weight, 5.0 to 40.0% by weight relative to the total weight of the cosmetic composition 30.0 wt%, 5.0 to 20.0 wt%, 10.0 to 50.0 wt%, 10.0 to 40.0 wt%, 10.0 to 30.0 wt%, for example, 10.0 to 20.0 wt% may be included. When included within the above range, it helps to moisturize and regenerate the skin, thereby having an excellent effect on skin softening.

In the present invention, the oil-absorbing powder may be at least one selected from the group consisting of silica, polymethylsilsesquioxane, and methiconesilsesquioxane crosspolymer.

In the present invention, the oil-absorbing powder is 0.1 to 10.0% by weight, 0.2 to 10.0% by weight, 0.3 to 10.0% by weight, 0.4 to 10.0% by weight, 0.5 to 10.0% by weight, 0.1 to 8.0% by weight relative to the total weight of the cosmetic composition, 0.2 to 8.0 wt%, 0.3 to 8.0 wt%, 0.4 to 8.0 wt%, 0.5 to 8.0 wt%, 0.1 to 6.0 wt%, 0.2 to 6.0 wt%, 0.3 to 6.0 wt%, 0.4 to 6.0 wt%, 0.5 to 6.0 wt%, 0.1-4.0 wt%, 0.2-4.0 wt%, 0.3-4.0 wt%, 0.4-4.0 wt%, 0.5-4.0 wt%, 0.1-2.0 wt%, 0.2-2.0 wt%, 0.3-2.0 wt% %, 0.4 to 2.0 wt%, 0.5 to 2.0 wt%, 0.1 to 1.0 wt%, 0.2 to 1.0 wt%, 0.3 to 1.0 wt%, 0.4 to 1.0 wt%, for example, 0.5 to 1.0 wt% can When included within the above range, there is an excellent effect in absorbing excess sebum in the skin.

In the present invention, the emulsifier may be one or more selected from the group consisting of lecithin, polyglyceryl-3methylglucose distearate, glyceryl stearate and PEG-40 stearate, but is not limited thereto.

In the present invention, the emulsifier is 0.1 to 10.0% by weight, 0.1 to 9.0% by weight, 0.1 to 8.0% by weight, 0.1 to 7.0% by weight, 0.1 to 6.0% by weight, for example, 0.1 to 5.0% by weight relative to the total weight of the cosmetic composition may be included. When included within the above range, it helps to stably disperse the oil phase and the aqueous phase in the formulation.

The present invention relates to a cosmetic composition for exfoliation comprising subtilisin, wherein subtilisin is stabilized in a non-aqueous formulation, and odor due to decomposition of subtilisin is significantly reduced.

1 is a graph showing the result of measuring the keratinous area before and after use according to an embodiment of the present invention.
2 is a graph illustrating the calculation of the keratin area increase/decrease rate (%) before and after use according to an embodiment of the present invention.
3 is a view showing a keratin tape photograph before and after exfoliation according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

manufacturing example. Preparation of Examples 1 to 3 and Comparative Examples 1 to 2

After weighing each aqueous phase (except moisture) and oil phase raw materials in a beaker, uniformly stir the aqueous phase and oil phase raw materials at room temperature for 5 minutes at 1,000 rpm using a disper to prepare the aqueous phase and oil phase did. The aqueous phase contained oil-absorbing powder, and the oil phase contained oil. Then, after adding the thickener to the aqueous phase, the mixture was uniformly mixed for 3 minutes at 3,000 rpm with a homogenizer. Then, after adding the oil phase, the mixture was uniformly mixed for 5 minutes at 3,000 rpm with a homogenizer. Then, each cosmetic composition was prepared by removing air bubbles. The components and contents of the cosmetic compositions of Examples 1 to 3 and Comparative Example 1 are shown in Table 1 below, and as Comparative Example 2, a general O/W cream type functional cosmetic composition was prepared with the components and contents shown in Table 2 below.

Ingredients (wt%) purpose Example 1 Example 2 Example 3 Comparative Example 1 Awards subtilisin exfoliant 0.4 0.1 0.7 - glycerin moisturizer To 100 To 100 To 100 To 100 silica Oil Absorbent Powder 0.5 0.5 0.5 0.5 thickener thickener 0.9 0.9 0.9 0.9 antiseptic antiseptic appropriate amount appropriate amount appropriate amount appropriate amount glucose moisturizer 0.04 0.04 0.04 0.04 paid Queensland Nut Oil oil 4.0 4.0 4.0 4.0 sweet almond oil oil 12.0 12.0 12.0 12.0 argan kernel oil oil 1.0 1.0 1.0 1.0 emulsifier emulsifier 0.3 0.3 0.3 0.3

Ingredients (wt%) purpose Comparative Example 2 subtilisin exfoliant 0.4 Purified water solvent To 100 glycerin moisturizer 8.0 Queensland Nut Oil oil 4.0 glucose moisturizer 0.04 emulsifier emulsifier 2.00 thickener thickener 0.15 tromethamine corrector 0.11 antiseptic antiseptic appropriate amount

Experimental Example 1. Evaluation of skin exfoliation effect, skin tone improvement, and moisturizing effect

Table 2 shows the results after 1 week of use for each product of Examples 1 to 3 and Comparative Examples 1 to 2 for 30 professional women aged 20-40 years. "Exfoliating effect" means that the skin surface is expressed smoothly without roughness. "Improve skin tone" means that the skin surface appears brighter when applied to the skin. "Moisturizing effect" refers to a moist feeling when applied to the skin. These three criteria increase the value of use as a cosmetic, and in particular, the exfoliating effect can be an important feature of a functional cosmetic.

<Evaluation criteria>

More than 24 women judged to be excellent: ◎

18-23 women judged to be excellent: ○

10-17 women judged to be excellent: △

Fewer than 10 women judged to be excellent: X

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 exfoliating effect ◎ ◎ ◎ △ X Skin tone improvement effect ◎ ○ ◎ X X moisturizing effect ◎ ○ ◎ ○ ○ Overall satisfaction ◎ ○ ◎ △ X

As can be seen in Table 3, Examples 1 to 3 were found to be superior to Comparative Examples 1 to 2 in terms of exfoliation effect, skin tone improvement effect, moisturizing effect, and overall satisfaction. In particular, Examples 1 and 3, in which a relatively large content of subtilisin was applied, showed the best overall feeling of use. It was found that Comparative Example 1 of the polyol composition in which subtilisin was not present and Comparative Example 2 in which subtilisin was present but O/W formulation had very poor exfoliation effect and skin tone improvement effect.

Experimental Example 2: Flavor evaluation

For each product of Examples 1 to 3 and Comparative Examples 1 to 2, 30 professional women aged 20-40 years were evaluated for scent after use, and the results are shown in Table 2.

<Evaluation criteria>

More than 24 women judged to be excellent: ◎

18-23 women judged to be excellent: ○

10-17 women judged to be excellent: △

Fewer than 10 women judged to be excellent: X

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 odor evaluation ◎ ◎ ◎ ◎ X

As can be seen in Table 4, Examples 1 to 3 did not have a specific odor, and it was found to be superior to Comparative Example 2. In particular, in Comparative Example 2, it was confirmed that the evaluation was very low due to the occurrence of an unpleasant peculiar odor due to the abnormal decomposition of subtilisin.

Experimental Example 3. Skin irritation test

A skin patch test was performed to investigate the skin irritation test. The subjects were selected as 19-55 years old, no skin disease at the test site, and a total of 20 subjects were selected. Specifically, after wiping and drying the upper arm, which is the patch site, with 70% ethanol, 20 μl of the sample was added and applied to the upper arm of the human body for 24 hours, and skin reaction was tested within 1 hour after the patch was removed. Then, after 24 hours and after 48 hours, the test was performed again in the same manner. The skin reaction was determined according to the following test standards, and the results are shown in Table 5.

<Evaluation criteria>

0: no response

±: mild positive reaction (erythema)

+: positive reaction (erythema)

++: Strong positive reaction (erythema, edema)

+++: Severe positive reaction (erythema, edema, blisters)

number of testers
(number of people) 0 ± + ++ +++ Example 1 20 18 2 0 0 0 Example 2 20 18 One One 0 0 Example 3 20 13 6 2 0 0 Comparative Example 1 20 17 One 0 0 0 Comparative Example 2 20 16 2 0 0 0

As can be seen in Table 5, Examples 1 to 3 and Comparative Examples 1 to 2 prepared by the manufacturing method of the present invention had low skin irritation and were confirmed to have low levels of irritation that can be used in the product.

Experimental Example 4. Stability evaluation

In order to measure the stability of the separation degree, each cosmetic composition is stored in a constant temperature bath maintained at 45 ° C and 25 ° C for 6 weeks in a constant temperature bath at 25 ° C or 45 ° C, and then checked weekly to check the phase stability. The results are shown in Table 5 below. (○: excellent emulsion stability, X: poor emulsion stability)

item Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Formulation ○ ○ ○ ○ ○ stability ○ ○ ○ ○ Ⅹ stability ○ ○ ○ ○ Ⅹ

As can be seen in Table 6, it was confirmed that Examples 1 to 3 and Comparative Example 1 were stable formulations with less separation.

Experimental Example 5. Confirmation of exfoliating effect

An experiment was conducted to evaluate the keratin removal and improvement efficacy of the cosmetic compositions for exfoliating the skin of Example 1 and Comparative Example 1. Specifically, 10 25-35 year old panelists were asked to wait in a constant temperature and humidity room for 10 minutes after washing the test site (face) with water. The keratin of the upper and lower cheeks of the test subject was collected using a keratin tape. Each product of Example 1 and Comparative Example 1 was spread on the same site twice a day, and keratin was collected again after 1 day of use. After analyzing the area of dead skin cells removed from the keratin tape using Image Pro Analyzer (Media Cybernetics, Inc., USA), the keratin improvement effect was checked before and after using the product, and the keratin area before and after use (%) The increase/decrease rate (%) of the hyperkeratinous area was calculated and shown in FIGS. 1 and 2 . In addition, the keratin tape before and after using the product was taken through C-cube (PIXIENCE, France), and the results of evaluation of keratin improvement before and after use are shown in FIG. 3 .

Example 1 Comparative Example 1 Keratin area before use (pixel) 1155000 1135000 After 1 day of use, the keratin area (pixel) 1140000 1290000

Example 1 Comparative Example 1 keratin area (pixel) increase/decrease rate (%) -1.3 13.7

As can be seen in FIG. 3 and Tables 7 to 8, it was confirmed that Example 1 exhibited a very excellent exfoliating effect within one day.

Claims (13)

A cosmetic composition for exfoliating dead skin cells comprising subtilisin. The cosmetic composition for exfoliation according to claim 1, wherein the subtilisin is contained in an amount of 0.1 to 2.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition of claim 1, wherein the cosmetic composition further comprises at least one selected from the group consisting of a moisturizer, a thickener, an oil, an oil-absorbing powder, and an emulsifier. The cosmetic composition for exfoliation according to claim 3, wherein the moisturizing agent is at least one selected from the group consisting of 1,3-butylene glycol, glycerin, diglycerin, glucose and propylene glycol. The cosmetic composition for exfoliating the dead skin according to claim 3, wherein the moisturizing agent is contained in an amount of 1.0 to 90.0% by weight based on the total weight of the cosmetic composition. The method according to claim 3, wherein the thickener is at least one selected from the group consisting of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, sodium acrylate copolymer, and polyacrylate crosspolymer-6. , a cosmetic composition for exfoliating dead skin cells. The cosmetic composition for exfoliation according to claim 3, wherein the thickener is included in an amount of 0.1 to 5.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for exfoliation of claim 3, wherein the oil is at least one selected from the group consisting of Queensland nut oil, sweet almond oil, squalane and argan kernel oil. The cosmetic composition for exfoliation according to claim 3, wherein the oil is included in an amount of 1.0 to 50.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for exfoliation according to claim 3, wherein the oil-absorbing powder is at least one selected from the group consisting of silica, polymethylsilsesquioxane, and methiconesilsesquioxane crosspolymer. The cosmetic composition for exfoliation according to claim 3, wherein the oil-absorbing powder is contained in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for exfoliation according to claim 3, wherein the emulsifier is at least one selected from the group consisting of lecithin, polyglyceryl-3methylglucose distearate, glyceryl stearate, and PEG-40 stearate. The cosmetic composition for exfoliation according to claim 3, wherein the emulsifier is included in an amount of 0.1 to 10.0% by weight based on the total weight of the cosmetic composition.

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