KR20210111449A - manufacturing method of powder green tea - Google Patents
manufacturing method of powder green tea Download PDFInfo
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- KR20210111449A KR20210111449A KR1020200026390A KR20200026390A KR20210111449A KR 20210111449 A KR20210111449 A KR 20210111449A KR 1020200026390 A KR1020200026390 A KR 1020200026390A KR 20200026390 A KR20200026390 A KR 20200026390A KR 20210111449 A KR20210111449 A KR 20210111449A
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- South Korea
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- malt
- weight
- supernatant
- parts
- saccharification
- Prior art date
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- 238000004519 manufacturing process Methods 0.000 title abstract description 19
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- 235000009569 green tea Nutrition 0.000 title abstract description 11
- BPJYAXCTOHRFDQ-UHFFFAOYSA-L tetracopper;2,4,6-trioxido-1,3,5,2,4,6-trioxatriarsinane;diacetate Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].CC([O-])=O.CC([O-])=O.[O-][As]1O[As]([O-])O[As]([O-])O1.[O-][As]1O[As]([O-])O[As]([O-])O1 BPJYAXCTOHRFDQ-UHFFFAOYSA-L 0.000 title description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/20—Malt products
- A23L7/25—Fermentation of cereal malt or of cereal by malting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/214—Tea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/24—Heat, thermal treatment
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
본 발명은 말차 식혜의 제조방법에 관한 것으로서, 더욱 상세하게는 고두밥 없이 엿기름만으로 당화시킨 후 말차를 첨가하여 건강에 유용하면서 기호도가 우수한 말차 식혜의 제조방법에 관한 것이다. The present invention relates to a method for producing matcha sikhye, and more particularly, to a method for producing matcha sikhye useful for health and excellent in taste by adding matcha after saccharifying it with malt alone without godubap.
식혜는 쌀을 쪄서 엿기름 추출액을 붓고 삭힌 다음 밥알은 냉수에 헹구어 건져 놓고, 그 물에 설탕과 생강을 넣고 끓여 식힌 다음 상기 밥알을 띄워 만든 한국 고유의 전통식품이다. Sikhye is a traditional Korean traditional food made by steaming rice, pouring malt extract, fermenting it, rinsing the grains of rice in cold water, adding sugar and ginger to the water, boiling it, and then floating the grains of rice.
종래의 식혜 제조방법은 지역과 가정에 따라서 약간의 차이는 있으나, 일정량의 엿기름을 물에 침지시켜 잘 주무른 후에, 그 상등액을 취하여 50분 정도 불려서 찐 쌀에 붓고 따뜻하게 보온하여 효소에 의한 당화가 일어나게 하였다가 쌀알이 5∼10개 정도 떠오르면 이를 당화의 종점으로 하여 설탕 등을 가하고 끓여서 제조하는 것이 일반적이다. The conventional sikhye manufacturing method differs slightly depending on the region and household, but after immersing a certain amount of malt in water and kneading it well, take the supernatant, soak it for 50 minutes, pour it into steamed rice, and keep it warm to cause saccharification by enzymes. However, when 5 to 10 grains of rice float up, it is common to use this as the end point of saccharification, add sugar, etc., and boil it.
밥을 재료로 만든 음료인 식혜는 영양도 좋고 풍미가 있어서 각 지방의 전통 제조방법에 의해 맛과 풍미가 다양한 식혜로 만들어져 애용되었다. 특히 최근에는 식혜의 제조과정에서 다양한 물질을 첨가하여 기능성을 강화시키고자 하는 식혜들이 개발되고 있다. Sikhye, a beverage made from rice, is nutritious and flavorful, so it was made into sikhye with a variety of tastes and flavors using traditional manufacturing methods in each region. In particular, recently, sikhye is being developed to enhance the functionality by adding various substances in the manufacturing process of sikhye.
대한민국 공개특허 제10-2019-0090097호에는 안동참마식혜 및 이의 제조방법이 개시되어 있고, 대한민국 공개특허 제10-2014-0134991호에는 토봉령을 이용한 식혜 및 그 제조방법이 개시되어 있다. Korean Patent Laid-Open No. 10-2019-0090097 discloses Andong yam sikhye and a manufacturing method thereof, and Korean Patent Laid-Open No. 10-2014-0134991 discloses sikhye using Tobongryeong and a manufacturing method thereof.
하지만, 상기 식혜들은 참마나 토봉령과 같이 첨가된 물질이 식혜와 잘 어우러지지 못해 기호도를 향상시키지 못하는 문제점이 있다. 또한, 고두밥을 첨가하여 당화시키는 종래의 방법은 식혜가 쉽게 변질이 되어 그 맛과 향이 떨어지는 문제점이 있었다.However, the sikhye has a problem in that the added substances such as yam or Tobongryeong do not harmonize well with the sikhye, so that the preference cannot be improved. In addition, the conventional method of adding godubap to saccharify sikhye has a problem in that the taste and flavor are deteriorated because the sikhye is easily deteriorated.
본 발명은 상기의 문제점을 개선하고자 창출된 것으로서, 고두밥 없이 엿기름만으로 당화시킨 후 말차를 첨가하여 건강에 유용하면서 기호도가 우수한 말차 식혜의 제조방법을 제공하는 데 그 목적이 있다.The present invention was created to improve the above problems, and it is an object of the present invention to provide a method for producing matcha sikhye which is useful for health and excellent in taste by adding matcha after saccharification of malt alone without godubap.
상기의 목적을 달성하기 위한 본 발명의 말차 식혜의 제조방법은 20 내지 30℃의 물에 엿기름을 침지시켜 1 내지 2시간 동안 불리는 엿기름침지단계와; 상기 엿기름침지단계 후 상기 엿기름이 침지된 물을 체에 밭쳐 찌꺼기를 제거하여 엿기름추출액을 수득하는 거름단계와; 상기 엿기름추출액을 30 내지 90분 동안 정치시켜 고액분리하는 고액분리단계와; 상기 고액분리단계에서 분리된 상등액을 5 내지 10시간 동안 60 내지 70℃로 유지하여 상기 상등액을 당화시키는 당화단계와; 상기 당화단계에서 수득한 당화액을 10 내지 30분 동안 끓이는 가열단계와; 상기 가열단계 후 끓인 당화액을 식힌 다음 말차를 첨가하는 첨가단계;를 포함한다.In order to achieve the above object, the method for preparing matcha sikhye of the present invention comprises the steps of immersing malt in water at 20 to 30° C. and immersing it in malt for 1 to 2 hours; a filtering step of obtaining a malt extract by sieving the malt-immersed water through a sieve after the malt immersion step; a solid-liquid separation step of solid-liquid separation by allowing the malt extract to stand still for 30 to 90 minutes; a saccharification step of saccharifying the supernatant by maintaining the supernatant separated in the solid-liquid separation step at 60 to 70° C. for 5 to 10 hours; a heating step of boiling the saccharification solution obtained in the saccharification step for 10 to 30 minutes; and an addition step of cooling the boiled saccharification solution after the heating step and then adding matcha tea.
상기 가열단계는 상기 당화액에 표고버섯 우린물을 첨가한 후 끓인다.In the heating step, shiitake mushroom broth is added to the saccharification solution and then boiled.
상기 당화단계는 상기 상등액 100중량부에 대하여 혼합발효추출액 10 내지 30중량부를 첨가하여 당화시키며, 상기 혼합발효추출액은 a)돼지감자(Helianthus tuberosus)의 덩이줄기와 섬개별꽃(Pseudostellaria heterophylla)의 잎을 혼합한 혼합물 100중량부에 대하여 설탕 10 내지 30중량부를 혼합한 후 유용미생물군 배양액 2 내지 10중량부를 첨가하여 20 내지 40℃에서 30 내지 90일 동안 발효시켜 발효물을 수득하는 단계와, b)상기 발효물을 믹서기로 갈아 발효분쇄물을 수득하는 단계와, c)상기 발효분쇄물에 추출용매를 중량비로 4 내지 10배를 가한 후 80 내지 120℃에서 2 내지 6시간 동안 추출하여 수득한다. The saccharification step is saccharification by adding 10 to 30 parts by weight of the mixed fermentation extract with respect to 100 parts by weight of the supernatant, and the mixed fermentation extract is a) a tuber of a pig potato ( Helianthus tuberosus ) and leaves of Pseudostellaria heterophylla ) After mixing 10 to 30 parts by weight of sugar with respect to 100 parts by weight of the mixed mixture, adding 2 to 10 parts by weight of a useful microbial group culture medium and fermenting at 20 to 40° C. for 30 to 90 days to obtain a fermented product, b; ) Grinding the fermented product with a mixer to obtain a fermented product; c) adding an extractant 4 to 10 times by weight to the fermented product and then extracting at 80 to 120° C. for 2 to 6 hours to obtain .
상술한 바와 같이 본 발명은 고두밥을 넣지 않고 엿기름 추출액만으로 당화시킨다. 이는 고두밥을 넣어 당화시키는 통상적인 식혜의 제조방법과 차이가 있다. 이와 같이 고두밥을 넣지 않고 엿기름 추출액만으로 당화시켜 식혜를 제조하므로 고두밥 첨가로 인한 문제점, 즉 색깔이 탁해지고 쉽게 변질이되어 맛과 향이 저하되는 것을 방지할 수 있다. As described above, the present invention saccharifies only the malt extract without adding godubap. This is different from the conventional manufacturing method of sikhye in which godubap is added and saccharified. In this way, since sikhye is manufactured by saccharifying only malt extract without adding godubap, it is possible to prevent problems caused by the addition of godubap, that is, the color becomes cloudy and easily deteriorates, thereby reducing the taste and flavor.
또한, 본 발명은 식혜의 제조공정에서 말차를 첨가하여 건강에 유용함과 동시에 기호도가 우수한 말차 식혜를 제공할 수 있다. In addition, the present invention can provide matcha sikhye useful for health and excellent in taste by adding matcha tea in the manufacturing process of sikhye.
또한, 본 발명은 말차 외에 혼합발효추출액을 더 첨가함으로써 식혜의 향과 맛을 오랫동안 유지시킴과 동시에 식혜의 기능성을 높일 수 있다. In addition, the present invention can enhance the functionality of sikhye while maintaining the flavor and taste of sikhye for a long time by adding a mixed fermented extract in addition to matcha tea.
이하, 본 발명의 바람직한 실시 예에 따른 말차 식혜의 제조방법에 대하여 설명한다.Hereinafter, a method for manufacturing matcha sikhye according to a preferred embodiment of the present invention will be described.
본 발명의 일 예에 따른 말차 식혜의 제조방법은 20 내지 30℃의 물에 엿기름을 침지시켜 1 내지 2시간 동안 불리는 엿기름침지단계와, 엿기름침지단계 후 상기 엿기름이 침지된 물을 체에 밭쳐 찌꺼기를 제거하여 엿기름추출액을 수득하는 거름단계와, 엿기름추출액을 30 내지 90분 동안 정치시켜 고액분리하는 고액분리단계와, 고액분리단계에서 분리된 상등액을 5 내지 10시간 동안 60 내지 70℃로 유지하여 상등액을 당화시키는 당화단계와, 당화단계에서 수득한 당화액을 10 내지 30분 동안 끓이는 가열단계와, 가열단계 후 끓인 당화액을 식힌 다음 말차를 첨가하는 첨가단계를 포함한다. 각 단계별로 구체적으로 살펴본다.The manufacturing method of matcha sikhye according to an embodiment of the present invention comprises a malt immersion step called for 1 to 2 hours by immersing malt in water at 20 to 30 ° C. A filtering step to obtain a malt extract by removing It includes a saccharification step of saccharifying the supernatant, a heating step of boiling the saccharified solution obtained in the saccharification step for 10 to 30 minutes, and an addition step of cooling the boiled saccharified solution after the heating step and then adding matcha. Let's look at each step in detail.
1. 엿기름침지단계1. Malt immersion step
먼저, 물에 엿기름을 침지시켜 물에 불린다. First, the malt is immersed in water and soaked in water.
엿기름은 싹을 튀운 보리를 말린 것으로서, 전분을 당화시키는 당화효소를 함유한다. Malt is dried sprouted barley and contains saccharifying enzymes that saccharify starch.
물의 온도는 20 내지 30℃가 적절한다. 물에 엿기름을 침지시켜 1 내지 2시간 동안 불린다. 그리고 물에 충분히 불린 다음 손으로 물에 잠긴 엿기름을 5분 정도 주물러 준다. 이는 엿기름의 성분이 물에 잘 우러나도록 하기 위함이다. The temperature of the water is preferably 20 to 30°C. Soak malt in water for 1 to 2 hours. After soaking in enough water, rub the soaked malt with your hands for about 5 minutes. This is to make the malt ingredients well excreted in water.
2. 거름단계2. Fertilization stage
다음으로, 엿기름이 침지된 물을 걸러서 찌꺼기와 엿기름추출액을 분리한다. Next, the malt extract is separated from the residue by filtering the water in which the malt is immersed.
통 위에 체를 얹은 다음 체에 엿기름이 침지된 물을 부으면 엿기름 찌꺼기는 체에 걸러지고, 액체 상태의 엿기름추출액은 체를 통과하여 통에 모아진다. When a sieve is placed on the sieve and the water immersed in malt is poured into the sieve, the malt residue is filtered through a sieve, and the malt extract in liquid state passes through the sieve and is collected in the tub.
3. 고액분리단계3. Solid-liquid separation step
다음으로, 엿기름추출액을 고액분리하여 상등액을 수득한다.Next, the malt extract is subjected to solid-liquid separation to obtain a supernatant.
통에 들어 있는 엿기름추출액을 30 내지 90분 동안 그대로 두어 정치시킨다. 30 내지 90분 동안 정치시키면 비중의 차이에 의해 비교적 무거운 고형분은 하층으로 침강되어 맑은 상등액과 분리된다. Let the malt extract in the barrel stand for 30 to 90 minutes. When left still for 30 to 90 minutes, the relatively heavy solids settle to the lower layer due to the difference in specific gravity and are separated from the clear supernatant.
4. 당화단계4. Saccharification step
다음으로, 고액분리단계에서 분리된 상등액을 당화시켜 당화액을 얻는다. Next, the supernatant separated in the solid-liquid separation step is saccharified to obtain a saccharified solution.
통상적인 식혜는 고두밥을 넣어 당화시키고 있으나, 본 발명은 고두밥을 넣지 않고 엿기름 추출액으로부터 분리된 상등액만으로 당화시킨다. 따라서 본 발명은 고두밥 첨가로 인한 문제점, 즉 색깔이 탁해지고 쉽게 변질이되어 맛과 향이 저하되는 것을 방지할 수 있다. Conventional Sikhye is saccharified by putting godubap, but in the present invention, godubap is not added and only the supernatant separated from the malt extract is saccharified. Therefore, the present invention can prevent the problem caused by the addition of godubap, that is, the color becomes cloudy and the quality is easily changed, and thus the taste and flavor are reduced.
상등액은 5 내지 10시간 동안 60 내지 70℃로 유지하여 당화시킬 수 있다. 상등액은 엿기름에 함유된 전분과 효소에 의해 당화된다. The supernatant can be saccharified by maintaining it at 60 to 70° C. for 5 to 10 hours. The supernatant is saccharified by the starch and enzymes contained in the malt.
그리고 단맛을 높이기 위해 상등액에 설탕을 첨가하여 당화시킬 수 있다. 이 경우 상등액 100중량부에 대하여 설탕 10 내지 20중량부를 첨가할 수 있다. In addition, sugar may be added to the supernatant to saccharify it to increase sweetness. In this case, 10 to 20 parts by weight of sugar may be added based on 100 parts by weight of the supernatant.
5. 가열단계5. Heating step
다음으로, 당화액을 10 내지 30분 동안 끓인다. 이는 효소를 실활시킴과 동시에 엿기름 특유의 냄새를 제거하기 위함이다. Next, the saccharification solution is boiled for 10 to 30 minutes. This is to inactivate the enzyme and at the same time remove the peculiar smell of malt.
당화액을 끓이는 동안 발생된 거품은 걷어서 제거한다. Remove the foam generated while boiling the saccharification solution by rolling it.
한편, 가열단계는 당화액에 표고버섯 우린물을 첨가한 후 끓일 수 있다. 가령, 당화액 100중량부에 대하여 표고버섯 우린물 5 내지 10중량부를 첨가할 수 있다. 표고버섯 우린물을 첨가할 경우 식혜의 감칠맛을 더할 수 있다. Meanwhile, in the heating step, shiitake mushroom broth may be added to the saccharification solution and then boiled. For example, 5 to 10 parts by weight of shiitake mushroom broth may be added based on 100 parts by weight of the saccharification solution. If you add shiitake mushroom broth, you can add the umami taste of sikhye.
표고버섯 우린물을 얻기 위해, 물 100중량부에 대하여 말린 표고버섯 10 내지 20중량부를 혼합한 다음 실온(20~30℃)에서 1 내지 2시간 동안 놔두어 우려낸다. 우린 후 체로 걸러내어 표고버섯 우린물을 얻는다. To obtain shiitake broth, 10 to 20 parts by weight of dried shiitake mushrooms are mixed with respect to 100 parts by weight of water, and then left to brew at room temperature (20 to 30° C.) for 1 to 2 hours. We then filter through a sieve to obtain shiitake mushroom broth.
6. 첨가단계6. Addition step
다음으로, 끓인 당화액을 식힌 다음 말차 가루를 첨가한다. Next, cool the boiled saccharification solution and then add matcha powder.
말차(powder green tea)는 녹차 잎을 말린 후 갈아서 만든 가루차의 일종이다. 차광재배한 녹차로부터 수확하여 줄기를 제거한 잎을 건조시킨 후 선별과정을 거쳐 분쇄기를 이용하여 분쇄하여 말차를 얻는다. 말차는 통상적인 녹차보다 카페인은 적으면서 풍부한 항산화 물질을 함유하고 있으며, 다이어트 식품에 주로 쓰는 L-테아닌, 유해산소 예방에 효과가 있는 베타카로틴 등 건강에 이로운 성분들이 많이 함유된 것으로 알려져 있다. 또한, 말차에 함유된 떫은 맛 성분인 카테킨은 강력한 항바이러스 및 항균 효과를 가지므로 바이러스 및 유해세균에 대한 활성 억제에 효과적인 것으로 알려져 있다. Matcha (powder green tea) is a type of powdered tea made by drying green tea leaves and then grinding them. The leaves are harvested from green tea grown under shading, dried, and then subjected to a screening process and then pulverized using a grinder to obtain matcha tea. Matcha tea contains less caffeine than green tea and contains abundant antioxidants, and is known to contain many beneficial ingredients such as L-theanine, which is mainly used in diet foods, and beta-carotene, which is effective in preventing free radicals. In addition, catechin, an astringent component contained in matcha, has strong antiviral and antibacterial effects and is known to be effective in inhibiting activity against viruses and harmful bacteria.
말차에 제조에 사용되는 녹차는 전라남도 보성 지역에서 재배한 것을 이용할 수 있다. Green tea used in the manufacture of matcha can be grown in Boseong, Jeollanam-do.
당화액 100중량부에 대하여 말차 2 내지 10중량부를 첨가할 수 있다. 말차를 첨가한 후 잘 섞으면 본 발명의 말차 식혜가 제조된다. 2 to 10 parts by weight of matcha may be added based on 100 parts by weight of the saccharification solution. Matcha sikhye of the present invention is prepared by mixing well after adding matcha.
말차는 미세한 가루 형태이므로 말차를 당화액에 그대로 첨가할 경우 말차가 당화액 중에 균일하게 분산되기 어렵다. 따라서 말차를 미리 액체에 개어둔 상태로 당화액에 첨가하는 것이 바람직하다. Since matcha is in the form of a fine powder, it is difficult to uniformly disperse the matcha in the saccharified liquid when the matcha is added to the saccharified liquid as it is. Therefore, it is preferable to add matcha to the saccharification liquid in a state in which the matcha has been previously opened in the liquid.
일 예로, 끓여서 식힌 당화액에서 일정량을 덜어 그릇에 담는다. 그리고 그릇에 말차를 투입한 다음 당화액에 골고루 섞어 이긴다. 이때 말차와 당화액의 비율은 중량비로 1:3이 적절하다. 이렇게 별도의 당화액에 이겨서 개어둔 말차를 남아있는 당화액에 첨가하여 잘 저어주면 말차가 당화액과 골고루 혼합된다. For example, take a certain amount from the boiled and cooled saccharification solution and put it in a bowl. Then, put matcha in a bowl and mix evenly with the saccharification solution. In this case, the ratio of matcha to saccharified solution is preferably 1:3 by weight. The matcha that has been beaten in a separate saccharified solution is added to the remaining saccharified solution and stirred well to evenly mix the matcha with the saccharified solution.
상술한 바와 같이 말차를 첨가하여 제조한 식혜는 건강에 유용함과 동시에 말차 특유의 색상을 띠어 기호도가 우수하다. 또한, 말차에 함유된 유용성분들에 의해 식혜의 기능성을 높일 수 있다. As described above, sikhye prepared by adding matcha tea is useful for health, and at the same time has a unique color of matcha and has excellent taste. In addition, the functionality of sikhye can be enhanced by the useful ingredients contained in matcha.
한편, 본 발명은 상술한 당화단계에서 혼합발효액을 첨가하여 당화시킬 수 있다. 가령, 상등액 100중량부에 대하여 혼합발효추출액 10 내지 30중량부를 첨가하여 60 내지 70℃에서 5 내지 10시간 동안 당화시켜 당화액을 얻을 수 있다. On the other hand, the present invention can be saccharified by adding a mixed fermentation solution in the above-described saccharification step. For example, by adding 10 to 30 parts by weight of the mixed fermentation extract with respect to 100 parts by weight of the supernatant, saccharification can be obtained by saccharification at 60 to 70° C. for 5 to 10 hours.
혼합발효추출액은 돼지감자와 섬개별꽃의 혼합물을 발효시킨 후 추출한 것이다. 본 발명에서 돼지감자는 덩이줄기를 이용하며, 섬개별꽃은 잎을 이용한다. The mixed fermented extract is extracted after fermenting a mixture of pork potato and samosaurus. In the present invention, the pork potato uses tubers, and the individual island flowers use leaves.
돼지감자(Helianthus tuberosus)는 뚱딴지라고도 불리며 노란색의 꽃을 피우는 국화과 식물로, 높이 15~3m로 자라며, 땅속줄기의 끝이 굵어져 덩이줄기가 발달하며 잎과 더불어 털이 있다. Potato pig ( Helianthus tuberosus ), also called tung calf, is a yellow-flowered asteraceae plant that grows to 15~3m in height.
돼지감자는 최근 그 추출물에 다량의 페놀성 화합물이 함유되어 있어 항산화 효과를 지니고 있는 것으로 규명된 바 있다. 또한, 돼지감자는 독성이 없는 것으로 알려져 있으며, 혈당을 안정화시키는 항당뇨 활성을 갖고 있어 현대인들의 성인병 질환인 당뇨병 예방과 치료에 매우 좋은 기능성 건강식품으로 이용가치가 높다. Pork potato has recently been found to have antioxidant effects because the extract contains a large amount of phenolic compounds. In addition, pork potatoes are known to be non-toxic and have anti-diabetic activity that stabilizes blood sugar, so they have a high use value as a functional health food that is very good for preventing and treating diabetes, an adult disease of modern people.
돼지감자는 80%의 수분과 15~20%의 탄수화물, 1~2%의 단백질, 그리고 무기질과 비타민 등으로 구성되어 있다. 보통의 식물이 탄수화물을 녹말의 형태로 저장하는 것과 달리 돼지감자는 탄수화물의 대부분을 이눌린(inulin)이라는 과당중합체 물질로 저장하는 특징이 있다. 이눌린은 돼지감자 원 중량의 14~19%를 차지할 정도로 그 비율이 높다. 이눌린은 돼지감자에 콜로이드상으로 존재하는 저장 다당류의 일종이다. 이눌린은 우리 몸의 소화효소에 의해 소화되지 않는 난소화성 식이섬유이다. 이러한 난소화성 특징 때문에 당뇨병 환자들에게 이눌린의 섭취가 권장되고 있다. 또한, 이눌린은 과당의 중합체이기 때문에 천연 감미원의 일종이다. Pork potato consists of 80% water, 15-20% carbohydrates, 1-2% protein, and minerals and vitamins. Unlike normal plants, which store carbohydrates in the form of starch, pork potatoes have the characteristic of storing most of the carbohydrates as fructose polymers called inulin. The proportion of inulin is high enough to account for 14-19% of the raw weight of pork potatoes. Inulin is a type of storage polysaccharide present in colloidal form in pork potatoes. Inulin is an indigestible dietary fiber that cannot be digested by digestive enzymes in our body. Because of these indigestible characteristics, inulin intake is recommended for diabetic patients. In addition, since inulin is a polymer of fructose, it is a kind of natural sweetener.
이와 같이 이눌린이 다량 함유된 돼지감자의 덩이줄기는 전분과 당분을 제공하여 식혜의 향과 맛을 크게 향상시킬 수 있다. As described above, the tuber of pork potatoes containing a large amount of inulin can significantly improve the flavor and taste of sikhye by providing starch and sugar.
섬개별꽃(Pseudostellaria heterophylla)은 숲속에서 자라는 석죽과의 여러해살이 풀로서, 줄기 끝 부분의 잎은 2쌍이 돌려난 것처럼 보이며, 넓은 난형으로 길이 3~6cm 및 폭 1~2cm의 연한 녹색이다. 섬개별꽃은 항산화 및 항균활성이 우수하여 식혜의 변질과 산패를 억제한다. 특히, 섬개별꽃과 돼지감자를 함께 이용할 경우 시너지 효과에 의해 항산화 및 항균활성을 크게 향상시킬 수 있다. Pseudostellaria heterophylla is a perennial grass of the family Pseudomonas that grows in the forest. The leaves at the end of the stem look like two pairs of leaves, and they are broad ovate, 3-6 cm long and 1-2 cm wide, light green. Isolate flower has excellent antioxidant and antibacterial activity, so it suppresses deterioration and rancidity of sikhye. In particular, when the isolate flower and pork potato are used together, the antioxidant and antibacterial activity can be greatly improved by the synergistic effect.
혼합발효추출액을 얻기 위해 먼저, 돼지감지의 덩이줄기와 섬개별꽃의 잎을 1:0.1~0.5의 중량비로 혼합한다. 그리고 돼지감지의 덩이줄기와 섬개별꽃의 잎을 혼합한 혼합물 100중량부에 대하여 설탕 10 내지 30중량부를 혼합한다. 그리고 혼합물 100중량부를 기준으로 유용미생물군 배양액을 더 첨가한다. To obtain the mixed fermented extract, first, the tuber of the pig persimmon and the leaf of the isola flower are mixed in a weight ratio of 1:0.1 to 0.5. And 10 to 30 parts by weight of sugar is mixed with respect to 100 parts by weight of a mixture of the tuber of the pig persimmon and the leaves of the isola flower. And the useful microbial group culture solution is further added based on 100 parts by weight of the mixture.
유용미생물군 배양액은 광합성 세균, 효모, 유산균, 방선균, 사상균 중 1종 이상의 유용미생물군(Effective Microoganims)을 배양한 것이다. 유용미생물군을 배양하기 위한 배지의 일 예로 쌀뜨물 18ℓ에 설탕이나 당밀 20g을 넣어 조성할 수 있다. 배지에 유용 미생물군을 접종한 후 30 내지 40℃에서 2 내지 8일 동안 배양하여 유용미생물군 배양액을 얻을 수 있다. 또한, 유용미생물군 배양액으로 상업화된 유용미생물군 제품을 시중에서 구입하여 이용할 수 있음은 물론이다The useful microbial group culture medium is a culture of one or more effective microbial groups among photosynthetic bacteria, yeast, lactic acid bacteria, actinomycetes, and filamentous fungi. As an example of a medium for culturing useful microorganisms, it can be prepared by adding 20 g of sugar or molasses to 18 liters of rice water. After inoculating the useful microbial group in the medium, it can be cultured at 30 to 40° C. for 2 to 8 days to obtain a useful microbial group culture solution. In addition, it goes without saying that commercialized useful microbial group products can be purchased and used in the market as a useful microbial group culture solution.
혼합물에 설탕과 유용미생물군 배양액을 첨가하여 혼합한 후 20 내지 40℃에서 30 내지 90일 동안 발효시킨다. 그리고 발효시킨 발효물을 믹서기로 갈아 발효분쇄물을 수득한다. 그리고 발효분쇄물에 추출용매를 중량비로 4 내지 10배를 가한 후 80 내지 120℃에서 2 내지 6시간 동안 추출한 후 여과하여 혼합발효추출액을 얻을 수 있다. After adding sugar and a culture medium of useful microorganisms to the mixture, the mixture is fermented at 20 to 40° C. for 30 to 90 days. Then, the fermented product is ground with a mixer to obtain a fermented product. And after adding 4 to 10 times the extraction solvent to the fermented crushed product by weight, extraction is performed at 80 to 120° C. for 2 to 6 hours, followed by filtration to obtain a mixed fermentation extract.
추출용매로 물, 탄소수 1 내지 4의 저급 알코올, 다가 알코올 또는 이들의 혼합물로부터 선택된 적어도 어느 하나를 이용할 수 있다. 탄소수 1 내지 4의 저급 알코올로 메탄올, 에탄올 등을 이용할 수 있고, 다가 알코올로 부틸렌글리콜 및 프로필렌글리콜, 펜틸렌글리콜 등을 이용할 수 있다. 그리고 혼합물로는 물 및 저급 알코올의 혼합물, 물 및 다가 알코올의 혼합물, 저급 알코올 및 다가 알코올의 혼합물, 또는 물 및 저급알코올 및 다가알코올의 혼합물을 이용할 수 있다.At least one selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol, or a mixture thereof may be used as the extraction solvent. Methanol, ethanol, etc. can be used as a C1-C4 lower alcohol, and butylene glycol, propylene glycol, pentylene glycol, etc. can be used as a polyhydric alcohol. And as the mixture, a mixture of water and a lower alcohol, a mixture of water and a polyhydric alcohol, a mixture of a lower alcohol and a polyhydric alcohol, or a mixture of water and a lower alcohol and a polyhydric alcohol may be used.
이하, 본 발명을 하기 실시예를 통하여 구체적으로 설명한다. 이는 본 발명을 보다 상세하게 설명하기 위한 것으로, 본 발명의 권리 범위를 하기의 실시 예로 한정하는 것은 아니다.Hereinafter, the present invention will be described in detail through the following examples. This is for explaining the present invention in more detail, and the scope of the present invention is not limited to the following examples.
(실시예1)(Example 1)
물에 엿기름을 침지시켜 90분 동안 불린 후 손으로 5분 주물은 다음 체로 걸러 엿기름추출액을 준비하였다. 엿기름추출액을 60분 동안 정치시켜 상등액을 분리한 다음 상등액 100중량부에 대하여 설탕 15중량부를 첨가하고 65℃에서 7시간 동안 당화시켰다. 그리고 당화액을 20분 동안 끓이면서 거품을 제거한 후 상온에서 식혔다. 그리고 식힌 당화액 100중량부에 대하여 말차(보성임병문말차, 임병문다원)6중량부를 첨가한 후 잘 섞어 말차 식혜를 제조하였다. Malt extract was prepared by immersing malt in water and soaking for 90 minutes, then casting for 5 minutes by hand, then sieving it through a sieve. After allowing the malt extract to stand for 60 minutes to separate the supernatant, 15 parts by weight of sugar was added to 100 parts by weight of the supernatant and saccharified at 65° C. for 7 hours. Then, the saccharification solution was boiled for 20 minutes to remove the foam, and then cooled at room temperature. Then, 6 parts by weight of matcha (Boseong Imbyeongmun Matcha, Imbyeongmun Teawon) was added to 100 parts by weight of the cooled saccharified solution, and then mixed well to prepare matcha sikhye.
(실시예2)(Example 2)
상기 실시예 1과 동일한 방법으로 준비한 상등액 100중량부에 대하여 혼합발효추출액 20중량부를 첨가하여 65℃에서 7시간 동안 당화시켰다. 그리고 당화액을 20분 동안 끓이면서 거품을 제거한 후 상온에서 식혔다. 그리고 식힌 당화액 100중량부에 대하여 말차 6중량부를 첨가한 후 잘 섞어 말차 식혜를 제조하였다. 20 parts by weight of the mixed fermentation extract was added to 100 parts by weight of the supernatant prepared in the same manner as in Example 1, and saccharification was carried out at 65° C. for 7 hours. Then, the saccharification solution was boiled for 20 minutes to remove the foam, and then cooled at room temperature. Then, 6 parts by weight of matcha was added to 100 parts by weight of the cooled saccharified solution, and then mixed well to prepare matcha sikhye.
혼합발효추출액은 아래와 같은 방법으로 준비하였다. The mixed fermentation extract was prepared in the following way.
돼지감지의 덩이줄기와 섬개별꽃의 잎을 1:0.3의 중량비로 혼합한 혼합물 100중량부에 대하여 설탕 20중량부와 유용미생물군 배양액(EM-1, (주)에버미라클) 2중량부를 첨가한 후 30℃에서 60일 동안 발효시켰다. 그리고 발효시킨 발효물을 믹서기로 갈아 발효분쇄물을 수득한 다음 물을 중량비로 6배를 가한 후 90℃에서 5시간 동안 추출한 후 여과하여 혼합발효추출액을 수득하였다. 20 parts by weight of sugar and 2 parts by weight of a useful microbial group culture solution (EM-1, Ever Miracle Co., Ltd.) are added to 100 parts by weight of a mixture of the tuber of the pig persimmon and the leaves of the isola flower in a weight ratio of 1:0.3. After that, it was fermented at 30°C for 60 days. Then, the fermented fermented product was ground with a mixer to obtain a fermented pulverized product, and then water was added 6 times by weight, extracted at 90° C. for 5 hours, and filtered to obtain a mixed fermentation extract.
(비교예)(Comparative example)
시중에서 상업화된 제품(팔도, 비락식혜)을 구입하여 이용하였다. Commercially commercialized products (Paldo, Birak Sikhye) were purchased and used.
위와 같이 제조된 실시예 1 및 2, 비교예의 새싹삼 음료을 대상으로 관능검사와 항산화활성 실험을 하였다. Sensory tests and antioxidant activity tests were performed on the ginseng drinks of Examples 1 and 2 and Comparative Examples prepared as above.
<관능검사><sensory test>
훈련된 패널을 대상으로 실시예 1 및 2, 비교예의 식혜에 대한 외관, 향, 맛, 종합기호도를 7점 척도법(매우 나쁘다 1점; 나쁘다 2점; 조금 나쁘다 3점; 보통이다 4점; 조금 좋다 5점; 좋다 6점; 매우 좋다 7점)으로 관능평가하였다. 관능검사의 결과는 하기 표 1에 나타내었다. Appearance, aroma, taste, and overall preference for Sikhye in Examples 1 and 2 and Comparative Examples were evaluated on a 7-point scale using a trained panel (1 point very bad; 2 points bad; 3 points slightly bad; 4 points moderate; a little Good 5 points; Good 6 points; Very good 7 points). The results of the sensory test are shown in Table 1 below.
상기 관능검사의 결과를 살펴보면, 실시예 1 및 2는 비교예에 비해 외관, 향, 맛, 종합기호도의 모든 면에서 점수가 훨씬 높게 나타났다. 따라서 본 발명은 말차를 첨가함으로써 기호도를 높일 수 있다. Looking at the results of the sensory test, Examples 1 and 2 showed much higher scores in all aspects of appearance, flavor, taste, and overall preference compared to Comparative Examples. Therefore, in the present invention, the preference can be increased by adding matcha.
그리고 실시예 1과 실시예 2를 비교하면, 외관에서는 유의적인 차이는 없는 것으로 나타났으나, 향과 맛, 종합기호도에서 실시예 2가 실시예 1보다 더 높은 것으로 나타났다. 이는 혼합발효추출액을 당화과정에서 첨가함으로써 기호도를 높인 결과로 보인다. And comparing Example 1 and Example 2, it was found that there was no significant difference in appearance, but Example 2 was found to be higher than Example 1 in aroma, taste, and overall preference. This seems to be the result of increasing the preference by adding the mixed fermentation extract during the saccharification process.
<항균실험><Antibacterial test>
혼합발효추출액의 항균효과를 살펴보기 위해 실시예 1 및 2의 식혜를 실온에서 5일간 방치하여 시료를 채취한 후 일반세균의 수를 측정하였다. 세균수 측정을 위해 채취한 시료를 멸균 균질기로 옮겨서 45mL의 0.5% 펩톤(peptone) 용액에 혼합하였다. 이 용액을 다시 순차적으로 0.5% 펩톤(peptone) 용액으로 희석하고 희석된 용액 0.1mL를 표준평판한천배지(plate count agar)에 도말하고 35℃에서 배양한 후 그 결과를 하기 표 2에 나타내었다. In order to examine the antibacterial effect of the mixed fermentation extract, the sikhye of Examples 1 and 2 were left at room temperature for 5 days to collect a sample, and then the number of general bacteria was measured. The sample collected for the measurement of the number of bacteria was transferred to a sterile homogenizer and mixed with 45 mL of 0.5% peptone solution. This solution was again sequentially diluted with 0.5% peptone solution, and 0.1 mL of the diluted solution was smeared on a standard plate count agar and cultured at 35 ° C. The results are shown in Table 2 below.
상기 표 2의 결과를 살펴보면, 혼합발효추출액이 첨가된 실시예 2가 실시예 1에 비해 미생물의 증식을 효과적으로 억제하는 것으로 확인되었다. 이에 따라 혼합발효추출액을 첨가하여 식혜를 제조할 경우 미생물에 의한 변질을 억제하여 장기 보존성을 높일 수 있을 것으로 기대된다. Looking at the results of Table 2, it was confirmed that Example 2 to which the mixed fermentation extract was added effectively inhibited the proliferation of microorganisms compared to Example 1. Accordingly, when sikhye is prepared by adding the mixed fermentation extract, it is expected that long-term preservation can be improved by suppressing deterioration caused by microorganisms.
<항산화 실험><Antioxidant experiment>
실시예 1 및 실시예 2, 비교예의 식혜를 시료로 사용하여 항산화 활성을 실험하였다. 항산화 활성은 1,1-diphenyl-2- picrylhydrazyl(DPPH) 라디칼에 대한 소거능으로 측정하였다. 시료 10g과 증류수 200mL을 혼합한 뒤 100℃에서 1시간씩 3반복 추출하였다. 각 추출물을 감압농축 후 -80℃에서 동결건조하여 최종 추출물로 제조하였다. 추출물을 증류수에 용해시킨 1mg/mL 농도의 추출액 600μL와 0.2mM DPPH 용액 300μL를 30분간 반응시켜 분광광도계로 517nm에서 흡광도를 측정하여 DPPH 라디칼 소거능(%)을 계산하였다. 라디칼 소거능 측정결과를 하기 표 3에 나타내었다. Antioxidant activity was tested using Sikhye of Examples 1 and 2 and Comparative Examples as samples. Antioxidant activity was measured by scavenging ability for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. After mixing 10 g of sample and 200 mL of distilled water, extraction was repeated three times at 100 °C for 1 hour each. Each extract was concentrated under reduced pressure and then freeze-dried at -80°C to prepare a final extract. 600 μL of an extract having a concentration of 1 mg/mL dissolved in distilled water and 300 μL of a 0.2 mM DPPH solution were reacted for 30 minutes, and absorbance was measured at 517 nm with a spectrophotometer to calculate DPPH radical scavenging ability (%). The measurement results of the radical scavenging ability are shown in Table 3 below.
상기 표 3의 결과를 참조하면, 비교예에 비해 실시예 1 및 실시예 2의 라디칼소거능이 더 높게 나타났다. 특히, 실시예 2는 비교예에 비해 2배 이상 더 높은 것으로 나타났다. 이에 따라 혼합발효추출액이 첨가된 식혜는 산패 억제 능력이 우수할 것으로 기대된다. Referring to the results of Table 3, the radical scavenging ability of Examples 1 and 2 was higher than that of Comparative Examples. In particular, Example 2 was found to be at least two times higher than that of Comparative Example. Accordingly, sikhye with mixed fermented extract is expected to have an excellent ability to inhibit rancidity.
이상, 본 발명은 실시 예를 참고로 설명하였으나 이는 예시적인 것에 불과하며, 당해 기술분야에서 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 실시 예가 가능하다는 점을 이해할 것이다. 따라서 본 발명의 진정한 보호 범위는 첨부된 청구범위에 의해서만 정해져야 할 것이다.As mentioned above, although the present invention has been described with reference to the embodiments, these are merely exemplary, and those of ordinary skill in the art will understand that various modifications and equivalent embodiments are possible therefrom. Accordingly, the true protection scope of the present invention should be defined only by the appended claims.
Claims (3)
상기 엿기름침지단계 후 상기 엿기름이 침지된 물을 체에 밭쳐 찌꺼기를 제거하여 엿기름추출액을 수득하는 거름단계와;
상기 엿기름추출액을 30 내지 90분 동안 정치시켜 고액분리하는 고액분리단계와;
상기 고액분리단계에서 분리된 상등액을 5 내지 10시간 동안 60 내지 70℃로 유지하여 상기 상등액을 당화시키는 당화단계와;
상기 당화단계에서 수득한 당화액을 10 내지 30분 동안 끓이는 가열단계와;
상기 가열단계 후 끓인 당화액을 식힌 다음 말차를 첨가하는 첨가단계;를 포함하는 것을 특징으로 하는 말차 식혜의 제조방법. Malt immersion step called for 1 to 2 hours by immersing the malt in water at 20 to 30 ℃;
a filtering step of obtaining a malt extract by sieving the malt immersed water through a sieve after the malt immersion step;
a solid-liquid separation step of solid-liquid separation by allowing the malt extract to stand still for 30 to 90 minutes;
a saccharification step of saccharifying the supernatant by maintaining the supernatant separated in the solid-liquid separation step at 60 to 70° C. for 5 to 10 hours;
a heating step of boiling the saccharification solution obtained in the saccharification step for 10 to 30 minutes;
and an addition step of cooling the boiled saccharified liquid after the heating step and then adding matcha tea.
상기 혼합발효추출액은 a)돼지감자(Helianthus tuberosus)의 덩이줄기와 섬개별꽃(Pseudostellaria heterophylla)의 잎을 혼합한 혼합물 100중량부에 대하여 설탕 10 내지 30중량부를 혼합한 후 유용미생물군 배양액 2 내지 10중량부를 첨가하여 20 내지 40℃에서 30 내지 90일 동안 발효시켜 발효물을 수득하는 단계와, b)상기 발효물을 믹서기로 갈아 발효분쇄물을 수득하는 단계와, c)상기 발효분쇄물에 추출용매를 중량비로 4 내지 10배를 가한 후 80 내지 120℃에서 2 내지 6시간 동안 추출하여 수득한 것을 특징으로 하는 말차 식혜의 제조방법.According to claim 1, wherein the saccharification step is saccharification by adding 10 to 30 parts by weight of the mixed fermentation extract with respect to 100 parts by weight of the supernatant,
The mixed fermentation extract is a) a mixture of tubers of a pig potato ( Helianthus tuberosus ) and leaves of Pseudostellaria heterophylla ( Pseudostellaria heterophylla ) After mixing 10 to 30 parts by weight of sugar with respect to 100 parts by weight of a mixture of useful microorganisms culture medium 2 to A step of fermenting for 30 to 90 days at 20 to 40 ℃ by adding 10 parts by weight to obtain a fermented product, b) grinding the fermented product with a mixer to obtain a fermented product, c) to the fermented product A method of producing matcha sikhye, characterized in that it is obtained by adding an extraction solvent 4 to 10 times by weight and then extracting at 80 to 120° C. for 2 to 6 hours.
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KR20010086175A (en) * | 2001-04-04 | 2001-09-10 | -- | The processing method of malt tea bag for Green tea Shik-hye making |
KR20090005690A (en) * | 2007-07-10 | 2009-01-14 | 박양순 | The method preparing for a sweet drink made fromfermented rice |
KR20110004229A (en) * | 2009-07-07 | 2011-01-13 | 김남호 | Sikhye using tangerine, green tea or white lotus and its manufacturing method |
KR20140134991A (en) | 2013-05-15 | 2014-11-25 | 김형한 | The making method of the rice nectar use smilax china |
KR20190090097A (en) | 2018-01-22 | 2019-08-01 | 안동대학교 산학협력단 | Andong charm-ma sikhye and manufacturing method thereof |
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KR20010086175A (en) * | 2001-04-04 | 2001-09-10 | -- | The processing method of malt tea bag for Green tea Shik-hye making |
KR20090005690A (en) * | 2007-07-10 | 2009-01-14 | 박양순 | The method preparing for a sweet drink made fromfermented rice |
KR20110004229A (en) * | 2009-07-07 | 2011-01-13 | 김남호 | Sikhye using tangerine, green tea or white lotus and its manufacturing method |
KR20140134991A (en) | 2013-05-15 | 2014-11-25 | 김형한 | The making method of the rice nectar use smilax china |
KR20190090097A (en) | 2018-01-22 | 2019-08-01 | 안동대학교 산학협력단 | Andong charm-ma sikhye and manufacturing method thereof |
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