KR20210084972A - reagent spraying apparatus and method for blood collection tube - Google Patents

Abstract

The present invention relates to a reagent dispensing device for blood collection and a reagent dispensing method using the same. By supplying the reagent directly from the micro-syringe to the injection nozzle without going through the distributor, the conventional method of dispensing the reagent using the distributor is improved, so that a very small amount of 10ul can be injected. In addition, the present invention relates to a reagent dispensing device for collecting blood vessels capable of dispensing quantitatively into 50 collection tubes at one time of injection, and a reagent dispensing method using the same. The reagent dispensing device for blood collection of the present invention comprises: a reagent tank in which a reagent is stored; a micro-syringe that sucks a reagent from reagent tank and discharges a certain amount; a spray nozzle connected to a micro-syringe to inject reagents into the blood collection vessel; and a reagent control valve that regulates supply and discharge of reagents.

Description

Reagent spraying apparatus and method for blood collection tube using the same

The present invention relates to a reagent dispensing device for blood collection and a reagent dispensing method using the same, and by directly supplying reagents from a microsyringe to a dispensing nozzle without going through a dispenser, improving the conventional method of dispensing reagents using a dispenser, It relates to a reagent injection device for blood collection vessels capable of injecting a very small amount and capable of injecting a fixed amount into 50 collection tubes at one injection, and a reagent injection method using the same.

With the rapid advancement of clinical laboratory technology, a wide range of blood tests, including serum biochemical tests, serum immunoassays, and blood counts, are contributing greatly to disease prevention and early diagnosis.

Blood tests take a certain amount of blood and place it in a container during the process of storage, transportation, and processing. Among the containers used at this time, the most common is a blood collection tube, and the inside of some blood collection tubes is decompressed so that blood can be easily sucked into the container by decompression.

There are several types of blood collection tubes depending on the use and size, but the tube collection tube has a structure in which the inlet of a cylindrical tube with an open top is sealed with a rubber packing and the outside of the rubber packing is covered with a cap. And on the outer peripheral surface of the tube, a label made of paper that can record product and blood collection-related information is attached, and EDTA-based or Heparin-based, sodium citrate, blood coagulation accelerator ( Clot Activator), etc., can be sprayed with a mist spray (spray spray) method.

In the blood collection tube or vacuum collection tube market, the products of BD Company of the United States and Greiner-Bio One (GBO) of Austria dominate the domestic and overseas market by more than 70%, and even the above two multinational companies have no knowledge of the reagent injection method. none.

Korean Patent Registration No. 10-1375120 discloses a reagent dispensing and dispensing device for collecting blood vessels.

The reagent dispensing and dispensing device includes a belt conveyor for carrying cartridges that are erected so that the upper part of which the plurality of tubes are opened faces upward, and a tube for aligning the cartridge moving along the belt conveyor at a position where the reagent is injected or dispensed. A holding unit, a nozzle unit including a plurality of nozzles to spray or dispense a reagent into the tube, and a nozzle driving unit for moving the nozzle unit to spray or dispense a reagent to all tubes coupled to the cartridge do.

And the nozzle unit includes a reagent tank in which the reagent is stored, a pump for pumping the reagent in the reagent tank, a plurality of nozzles for dispensing the reagent into the tube, and a dispenser for dispensing the reagent to the plurality of nozzles. include

In the case of the above-described reagent dispensing and dispensing device, dispensing is not a major problem, but when dispensing reagents, there was a problem that a very small amount of less than 10 μl was not injected. In addition, in the case of a high concentration reagent such as EDTA K2, the operating time (MTTR) In less than 10 minutes, the process of spraying for 5 minutes and washing with water for 5 minutes resulted in a decrease in production and the stress on the work of workers and inspectors was high, so the need for improvement was raised.

The role of the dispenser used in the existing reagent dispensing process is to distribute the reagent evenly into 25 to 50 units, but there are cases in which the dispensing may not be possible if distilled water (DW) or special reagent is injected in a small amount (10uL). This is a disadvantage that the inspector has to perform a complete inspection.

Republic of Korea Patent No. 10-1375120: Reagent dispensing and dispensing device for collecting blood vessels

The present invention was created to improve the above problems, there is no dispenser (manifold), injection is possible even at a minimum volume of 10uL, and a reagent injection device for blood collection vessels capable of stable reagent injection even after continuous injection, and a reagent injection method using the same Its purpose is to provide

A reagent injection device for blood collection vessels of the present invention for achieving the above object comprises: a reagent tank in which a reagent is stored; a micro-syringe for sucking a reagent from the reagent tank and discharging a predetermined amount; an injection nozzle connected to the micro-syringe to inject a reagent into the blood collection vessel; and a reagent control valve for controlling supply and discharge of the reagent.

And to achieve the above object, the reagent injection method for blood collection vessels of the present invention comprises the steps of supplying a reagent from a reagent tank in which the reagent is stored to a micro-syringe; introducing the reagent discharged from the micro-syringe into the injection nozzle; and injecting the reagent introduced into the injection nozzle into the inside of the collecting vessel.

As described above, in the present invention, a very small amount of 10ul can be injected compared to the conventional method using a distributor by directly supplying a reagent from a micro-syringe to the injection nozzle without going through a distributor, and also 50 pieces per injection. It can be injected in a quantitative amount into the vascular tube.

The conventional reagent injection device has a structure that cannot spray a very small amount of 10uL, but the present invention can spray even a very small amount of 10uL, which makes it possible to develop a new vacuum blood collection tube for a specific blood test. In the case of injecting 10uL from the existing reagent injection device, the inspector has to perform a complete inspection, which results in very low production efficiency. However, since the reagent injection device according to the present invention can spray even 10uL, the inspector does not have to do a full inspection. There is no need, thereby greatly improving the production efficiency.

In addition, in the prior art, when a high-concentration reagent such as EDTA K2 is sprayed, the reagent is crystallized between the inner nozzle (reagent nozzle) and the outer nozzle (compressed air nozzle or pneumatic nozzle) of the injection nozzle, resulting in nozzle clogging. The production efficiency is lowered because the washing operation has to be performed, but in the present invention, the nozzle clogging phenomenon does not occur.

Therefore, since the present invention can be continuously produced, productivity can be increased, stabilized mass production of blood collection vessels is possible, and new products with high added value can be developed.

1 is a reagent injection process diagram using a conventional reagent injection device,
Figure 2 is a schematic diagram showing the appearance of the reagent in the injection nozzle of the conventional reagent injection device,
3 is a reagent injection process diagram using a reagent injection device according to an embodiment of the present invention;
4 is a schematic diagram showing the state of the reagent in the injection nozzle of the reagent injection device according to an embodiment of the present invention.

Hereinafter, a reagent dispensing apparatus for blood collection and a reagent dispensing method using the same according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

The reagent injection device for a blood collection tube of the present invention includes a reagent tank in which a reagent is stored, a micro-syringe for sucking a reagent from the reagent tank and discharging a predetermined amount, a spray nozzle connected to the micro-syringe to inject the reagent into the blood collection tube, and a reagent; A reagent control valve that regulates the supply and discharge of

And the reagent injection method for blood collection tube of the present invention comprises the steps of supplying a reagent from a reagent tank in which the reagent is stored to a micro-syringe, introducing the reagent discharged from the micro-syringe into the injection nozzle, and collecting the reagent introduced into the injection nozzle. and injecting into the interior of the blood vessel.

1 is a schematic diagram of a reagent injection process for a vacuum collection tube according to a conventional method, where various reagents must be dispensed or sprayed according to the capacity according to the blood (whole blood, plasma, serum) test in the vacuum collection tube, where dispensing is a major problem However, the spraying (Micro Spray, Mist) is for micro-coating the inner wall of the PET tube, but when the reagent volume is less than 10uL and in the case of a 1ml vacuum collection tube (10uL spray), there is a problem that it is difficult to spray. Even if it does, it should be thoroughly tested. That is, the 10uL volume injection does not play a role in the distributor (manifold).

And, in the case of reagent injection by the conventional method, the shape of the reagent in the inner nozzle of the injection nozzle is not uniform as shown in FIG. 2 .

2 is an injection process diagram of the blood collection reagent injection device of the present invention, and the reagent injection device is capable of injection even in a very small amount of 10uL, for this reason, except for the dispenser, one reagent injection nozzle and one micro-syringe are directly connected. This is because it is possible to spray in a very small amount because it is sprayed with

In the case of the reagent injection according to the present invention, the shape of the reagent in the inner nozzle of the injection nozzle is uniform as shown in FIG. 4 .

And in the conventional case, 50 tubes can be sprayed at the same time during one injection. In the conventional reagent injection process, 25 distributors can be distributed and sprayed from one distributor, and 50 tubes can be simultaneously sprayed through two distributors. It can be sprayed, but the reagent spraying device of the present invention can spray 50 pieces at the same time.

As such, in the present invention, a very small amount of 10ul can be injected compared to the conventional method using a distributor by directly supplying a reagent from a micro-syringe to the injection nozzle without going through a distributor, and also 50 blood collection tubes at a time of injection It can be sprayed in a fixed quantity.

The conventional reagent injection device has a structure that cannot spray a very small amount of 10uL, but the present invention can spray even a very small amount of 10uL, which makes it possible to develop a new vacuum blood collection tube for a specific blood test. In the case of injecting 10uL from the existing reagent injection device, the inspector has to perform a complete inspection, which results in a very low production efficiency. However, since the reagent injection device according to the present invention can spray even 10uL, the inspector does not have to do a full inspection. There is no need, thereby greatly improving the production efficiency.

In addition, in the prior art, when a high-concentration reagent such as EDTA K2 is sprayed, the reagent is crystallized (solid) between the inner nozzle (reagent nozzle) and the outer nozzle (compressed air nozzle or pneumatic nozzle) of the injection nozzle, causing nozzle clogging, which causes periodic The production efficiency is lowered because the washing operation has to be performed, but in the present invention, the nozzle clogging phenomenon does not occur.

As mentioned above, although the present invention has been described with reference to one embodiment, it will be understood that this is only exemplary, and that various modifications and equivalent embodiments are possible therefrom by those of ordinary skill in the art. Accordingly, the true protection scope of the present invention should be defined only by the appended claims.

Claims (2)

a reagent tank storing reagents;
a micro-syringe for sucking a reagent from the reagent tank and discharging a predetermined amount;
an injection nozzle connected to the micro-syringe to inject a reagent into the blood collection vessel;
and a reagent control valve for controlling supply and discharge of the reagent. supplying a reagent from a reagent tank in which the reagent is stored to a micro-syringe;
introducing the reagent discharged from the micro-syringe into the injection nozzle;
and injecting the reagent introduced into the injection nozzle into the blood collection vessel.

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