KR20210068841A - Composition for anti-oxidant, anti-wrinkle and immune enhancement comprising bass extract - Google Patents

Abstract

The present invention relates to an antioxidant, anti-wrinkle and immune enhancement composition comprising a Micropterus salmoides extract. The Micropterus salmoides extract exhibits an excellent antioxidant effect through DPPH radical scavenging activity and ABTS scavenging activity, and exhibits an immune activation effect by increasing the production of nitric oxide (NO) that activates the immune response, so the composition of the present invention including the Micropterus salmoides extract can be usefully used as an antioxidant and anti-wrinkle cosmetic composition, a health functional food, and a composition for enhancing immune activity.

Description

Composition for anti-oxidant, anti-wrinkle and immune activity enhancement comprising a bath extract {Composition for anti-oxidant, anti-wrinkle and immune enhancement comprising bass extract}

The present invention relates to a composition for antioxidant, anti-wrinkle and immune activity enhancement comprising a bath extract, and specifically, a cosmetic composition for antioxidant and anti-wrinkle improvement comprising a bath extract, and a health functional food composition and immunity comprising a bath extract It relates to a composition for enhancing activity.

Bass ( Micropterus salmoides ) belongs to the family Centrarchidae of the order Perciformes, and its origin is in the southeastern part of the United States, and it was introduced to the world for the purpose of aquaculture and fishing. In Korea, bass was first introduced in 1973 by the Korea Fisheries Agency for the purpose of creating resources, and through a test study, its predation and its effect on the ecosystem during release were discussed.

Bass is a freshwater fish with a flat head and body and a long spindle-shaped body. In addition, the head is large, the eyes are relatively small, the snout is long and pointed, and the mouth is large, with the mandible protruding slightly ahead of the maxilla. There are two dorsal fins, and the center of the trailing edge of the caudal fin is concave inward. The back is dark blue, the belly is yellow, and there is a long blue-brown stripe in the center of the side of the body.

On the other hand, the bass contains 1.5 to 4 times more minerals such as calcium, phosphorus, and iron compared to other freshwater fish, so it is rich in nutrition and has only 10 to 30% fat, so it has a light taste. In addition, it contains a large amount of taurine, an amino acid that prevents aging, so it is treated as a high-quality fish in Japan, China, the United States, and Australia.

Bass is evaluated as having high nutritional value, but as the species diversity of fish decreases in the ecosystem to which the bass is introduced, and as a result, benthic organisms and algae become abundant, the structure of the food chain, the energy flow of the ecosystem, and habitat change are directly affected. · It has been reported to cause ecosystem disturbance through indirect effects. Due to these negative perceptions, studies using baths are low compared to their high nutritional value.

In addition, there is a disadvantage in that it is difficult to use the bass as food or food because the characteristic fishy smell is stronger than that of other fish species.

The present inventors screened the activity of the bath extract to increase the utilization of the bass classified as an ecosystem-disrupting species, and completed the present invention by identifying that the bath extract has an excellent antioxidant effect and immune activity enhancing effect.

Accordingly, an object of the present invention is to provide a cosmetic composition for antioxidants comprising a bath extract.

Another object of the present invention is to provide a cosmetic composition for improving wrinkles comprising a bath extract.

Another object of the present invention is to provide a health functional food composition for antioxidants comprising a bath extract.

Another object of the present invention is to provide a functional food composition for wrinkle improvement comprising a bath extract.

Another object of the present invention is to provide a composition for enhancing immune activity comprising a bath extract.

According to one aspect of the present invention, the present invention relates to a cosmetic composition for antioxidants comprising a bath extract.

According to another aspect of the present invention, the present invention relates to a cosmetic composition for improving wrinkles comprising a bath extract.

The bath used in the present invention is a freshwater fish that is evenly distributed throughout the country and is rich in minerals, proteins and lipids compared to other fish species. In particular, the bath is known to be rich in taurine compared to other freshwater or sea fish species, but it has a disadvantage in that it is difficult to use as food or food because of the characteristic fishy smell of the bath, which makes it difficult to use it as food or food.

In the present invention, the characteristic fishy smell of the bath is effectively removed through the extraction process, so that when it is included in a cosmetic composition or a health functional food composition, it is possible not to feel unpleasant in smell or flavor.

The bath extract of the present invention consists of a bath water, HCl solution, NaOH aqueous solution, straight or branched alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, hexane and diethyl ether. It may be a crude extract obtained by extraction with one or more solvents selected from the group.

According to one embodiment of the present invention, the bath extract of the present invention may be a crude extract obtained by extracting the bath with water, HCl solution, or aqueous NaOH solution as a solvent.

The water used to obtain the bath extract may be water having a temperature of room temperature (18 to 25° C.) or higher, for example, hot water at 50° C. or higher.

The bath extract according to the present invention may be a solvent fraction obtained by fractionating the crude solvent extract with an additional solvent.

The 'extract' includes a crude solvent extract, a specific solvent soluble extract (solvent fraction), and a solvent fraction of a crude solvent extract, and the bath extract may be in a solution, concentrate and/or powder form.

Specifically, the term 'extract' as used herein has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract.

That is, the bath extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc., additionally carried out The fractions obtained through various purification methods are also included in the bath extract of the present invention.

The bath extract used in the present invention may be prepared in the form of a concentrate and/or powder by additional processes such as vacuum distillation and freeze drying or spray drying.

The upper limit of the quantity of the bath extract contained in the composition of the present invention may be selected by those skilled in the art within an appropriate range.

The composition of the present invention exhibits an antioxidant effect by scavenging DPPH radicals and ABTS radicals, and thus can be used as an antioxidant composition.

As we age, aging is accelerated by various factors, including reactive oxygen species, which appear as wrinkles, a representative phenotype of aging. As a method to prevent aging, that is, wrinkles, reducing UV exposure, which accounts for 80% of the causes of aging, and sufficient supply of antioxidants are important prevention and treatment methods.

Antioxidants prevent aging by reducing oxidative stress in cells by removing reactive oxygen species, and each antioxidant affects aging mechanisms or mechanisms related to aging, such as collagen synthesis, with different functions, resulting in aging and its phenotype. It is known that it suppresses wrinkles and prevents skin aging by increasing repair activity by inducing the activation of repair proteins by an antioxidant effect on redox signaling in cells.

Therefore, the composition of the present invention exhibiting excellent antioxidant activity can be used for wrinkle improvement.

The composition for antioxidant and wrinkle improvement of the present invention may be prepared as a cosmetic composition.

When the composition of the present invention is prepared as a cosmetic composition, skin, skin softener, skin toner, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, eye cream, moisture cream, hand cream, essence, nutrition It may have any one formulation selected from essence, pack, cleansing foam, cleansing water, cleansing cream, body lotion, body cleanser, soap and powder, but is not limited thereto. The cosmetic composition consisting of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene. fatty acid esters of glycol, 1,3-butylglycol oil, glycerol fatty esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, etc. may be used.

The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobicity-inducing substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like. .

According to another aspect of the present invention, the present invention relates to a health functional food composition for antioxidants comprising a bath extract.

According to another aspect of the present invention, the present invention relates to a health functional food composition for improving wrinkles comprising a bath extract.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The amount of the active ingredient may be appropriately used depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of the health functional food. Examples of foods to which the health functional food composition can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, alcoholic beverages, vitamin complexes, and the like, and includes all health foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when it is prepared as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.) or sugar alcohols (eg, , xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent and the like used in beverages. The ratio of these added ingredients is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

According to another aspect of the present invention, the present invention relates to a composition for enhancing immune activity comprising a bath extract.

The bath extract of the present invention exhibits an immunoactive effect by increasing the production of nitric oxide (NO).

NO has various physiological functions such as platelet aggregation and neutrophil aggregation in the vascular system and metabolic regulation in skeletal muscle. synthesized by cells. In the inflammatory process, NO is involved in infection control, regulation of signaling and transcription factors, regulation of vascular responses, leukocyte migration, cytokine production, and apoptosis.

The bath extract appears to act as an immune activating factor that stimulates and activates NO, which plays an important role in immune enhancement, and thus can be used as a material helpful in improving immunity.

The composition for enhancing immune activity of the present invention may be prepared as a feed composition.

When prepared as a feed composition, it may include various feed ingredients known in the art in addition to the bath extract as an active ingredient.

The feed composition may be a feed composition for animals.

The animal is not particularly limited, but may preferably be a dog or a cat. In particular, in the case of dogs, the nutrients contained in the bath help the dog's skeletal development and growth development, and in the case of cats, the bath contains a large amount of taurine, so it is helpful to ingest the essential amino acid taurine.

The present invention relates to a composition for antioxidant, anti-wrinkle and immune activity enhancement comprising a bath extract. The bath extract exhibits an excellent antioxidant effect through DPPH radical scavenging activity and ABTS scavenging activity, and exhibits an immune activation effect by increasing the generation of NO (nitric oxide) that activates the immune response. It can be usefully used as a cosmetic composition for antioxidant and anti-wrinkle improvement, a health functional food, and a composition for enhancing immune activity.

Figure 1a is a result of measuring the cytotoxicity of the bath hot water extract (50 ℃).
Figure 1b is the result of measuring the cytotoxicity of the bath hot water extract (100 ℃).
Figure 1c is a result of measuring the cytotoxicity of the bath HCl extract.
Figure 1d is a result of measuring the cytotoxicity of the bath NaOH extract.
Figure 2 is the result of analyzing the active oxygen removal ability of the bath extract through the DPPH assay.
3 is a result of analyzing the antioxidant ability of the bath extract through the ABTS assay.
Figure 4 is a result of measuring whether the immune activation to the bath extract.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

manufacturing example. Preparation of Bath Extract

The flesh, skin, intestines, and head of the bath are crushed into 200-300 mesh to make a powder, and then distilled water (50℃, 100℃), 1% (v/v) HCl or 1% ( v/v) NaOH for 30 minutes and centrifugation for 3,000 g X 20 minutes to obtain a supernatant.

Example 1. Evaluation of cell safety of bath extracts

Human skin cells, HaCat cells, were seeded in 96-well plates at 5×10 ³ cells. After culturing at 37°C for 24 hours, dilute 4 types of bath extracts in DMEM cell culture medium at a ratio of 0, 1, 2.5, 5, 10, 25, 50, 100% (v/v), and then exchange the culture medium on the plate. did. After further culturing at 37°C for 24 hours, EZ-Cytox was added at a ratio of 10% (v/v) of the medium, and absorbance was measured at 450 nm after 30 minutes.

Except for the NaOH extract, the three extracts (50°C hot water extract, 100°C hot water extract and HCl extract) showed cytotoxicity at 50% (v/v) or more ( FIGS. 2a-2d ). When 100% (v/v) extract was treated, cytotoxicity was observed in all experimental groups. When the bath extract is used as a general feed, there is no possibility that the cells will be treated with more than 20% (v/v), so cell safety is considered to be very high. The 50℃ hot water extract and NaOH extract showed a strong cell proliferation effect up to 25% (v/v) treatment group.

Example 2. Evaluation of antioxidant activity of bath extract

2-1. Active oxygen removal activity

0.25 mM DPPH was dissolved in ethanol to make a final concentration of 0.0019 g/20 mL, and filtered with a 0.22 μm filter syringe. Four types of extracts were diluted with tertiary distilled water and prepared by concentration (0, 1, 10, 50, 100%). Distilled water was used as a negative control and ascorbic acid was used as a positive control. 350 μl of DPPH reagent was dispensed into the tube, and the same amount of control and extract samples were dispensed. In order to remove the retained color of the sample, 350 μl of distilled water was dispensed, and then the extract sample was dispensed in the same amount. After vortexing, the reaction was performed at room temperature for 20 minutes, and 200 μl each was dispensed in 3 repetitions in a 96-well plate. Absorbance was measured at 517 nm using an ELISA reader.

As a result, the 50 ℃ hot water extract showed high active oxygen removal activity from 50% (v/v) concentration, and the 100 ℃ hot water extract showed that the active oxygen removal effect decreased as the concentration gradually increased (FIG. 3). The HCl extract and NaOH extract started to show the active oxygen removal effect from 10% (v/v) concentration, and showed more than 50% activity of ascorbic acid (100 μM) at the highest concentration of 100% (v/v). In the 100% (v/v) extract, which is the highest concentration, the active oxygen scavenging activity was significantly higher in the HCl extract and the NaOH extract than in the hot water extract.

In conclusion, when the bath extract is used as a feed ingredient, it is thought that it will exhibit a high ability to remove active oxygen when it is decomposed by acid and basic fluid in the body of an animal and then transferred to cells.

2-2. Antioxidant activity evaluation (ABTS assay)

7 mM ABTS and 2.4 mM potassium persulfate were dissolved in tertiary distilled water, mixed, and reacted at room temperature for 12-16 hours while blocking light. The absorbance was measured at 414 nm in an ELISA reader and diluted with tertiary distilled water until a value of 1.5 ± 0.03. The extract was prepared by diluting with tertiary distilled water for each sample concentration.

Four types of extracts were diluted with tertiary distilled water and prepared by concentration (0, 1, 10, 50, 100%). Distilled water was used as a negative control and ascorbic acid was used as a positive control. 600 μl of DPPH reagent was dispensed into the tube, and 200 μl of the extract sample was dispensed. In order to remove the retained color of the sample, 600 μl of distilled water was dispensed, and then 200 μl of the extract sample was dispensed. After vortexing, the reaction was performed at room temperature for 10 minutes, and 200 μl each was dispensed in 3 repetitions in a 96-well plate. Absorbance was measured at 414 nm using an ELISA reader.

Unlike the DPPH analysis, it was confirmed that the antioxidant effect increased as the concentration increased in all samples (FIG. 3). The four extracts showed higher activity than ascorbic acid up to 50% concentration, and showed similar effects to ascorbic acid activity at the highest concentration of 100%. In conclusion, it is thought that the bath extract can have excellent effects on immunity and anti-aging because it exhibits excellent antioxidant effect.

Example 3. Evaluation of immune activation ability of bath extract

THP-1 cells were seeded in a 24-well plate, treated with a diluted extract sample and LPS (1 μg/ml) in the medium at a concentration of 10% or 20%, and cultured for 24 hours. After collecting the supernatant, it was mixed with Griess reagent 1:1, incubated at room temperature for 15 minutes, and absorbance was measured at 540 nm.

The immune activation effect of the feed containing the bath was confirmed by quantifying the amount of Nitric Oxide (NO) produced and secreted by immune activation using THP-1 cells, a human-derived monocyte cell line. Four types of extracts for the bath were diluted to a concentration without cytotoxicity, and incubated with LPS (1 μg/ml) for 24 hours. Regardless of the extraction method, it was confirmed that the NO value significantly increased compared to the control group. Therefore, it is determined that the bath extract induces immune activation (FIG. 4).

Claims (6)

A cosmetic composition for antioxidants comprising a bath extract.
The method of claim 1, wherein the extract is water, HCl solution, NaOH aqueous solution, a linear or branched alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, 1,3-butylene glycol, hexane and diethyl ether. An extract obtained by extraction with one or more solvents selected from the group consisting of, an antioxidant cosmetic composition.
A cosmetic composition for wrinkle improvement comprising a bath extract.
A health functional food composition for antioxidants comprising a bath extract.
A health functional food composition for wrinkle improvement comprising a bath extract.
A composition for enhancing immune activity comprising a bath extract.

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