KR20210060121A - composition for improving skin inflammation - Google Patents

Abstract

The present invention relates to a cosmetic composition having an anti-inflammatory effect and, more specifically, to a cosmetic composition having itching alleviation or anti-inflammatory effects by comprising herbal medicine complex extracts of Centella asiatica, Arctium minus, roots of Anemarrhena asphodeloides, seeds of Nelumbo nucifera, fruits of Forsythia suspensa and flowers of Lonicera japonica as active ingredients. The cosmetic composition having an anti-inflammatory effect according to the present invention is safe for the skin and has excellent anti-inflammatory effect, which is a skin trouble alleviation effect.

Description

Composition for improving skin inflammation}

The present invention relates to a cosmetic composition having an anti-inflammatory effect, and more particularly, to a cosmetic composition that can be safely used without side effects on the skin and has an anti-inflammatory effect that is a skin trouble relief effect.

Cosmetics are products used to protect the skin and to beautify and clean the skin, but its composition inevitably contains ingredients different from those for skin protection purposes. These ingredients include surfactants, preservatives, fragrances, sunscreens, pigments, as well as various other ingredients to impart efficacy and effects. These ingredients are generally known to cause various problems such as inflammation, rashes, and swelling on the skin. (Maibach. HI, Contact Dermatitis, 6. 369-404, 1980) In addition, sebum and sweat discharged from the body, cosmetic ingredients It is a well-known fact that heavy fatty acids, higher alcohols, proteins, etc., are decomposed into highly toxic substances by dermatophytes that exist on the skin, which may cause skin inflammation, and that even UV rays can cause skin inflammation. .

The inflammatory response is represented by five phenomena such as redness, tingling, burning, swelling, and tissue change. This is a physiological reaction to protect the living body from the invasion of foreign substances such as bacteria and mechanical damage. This inflammatory phenomenon causes a large increase in various types of polykaryotic leukocytes (PMNs) and immune substances, and these increased cells secrete various types of proteolytic enzymes and cytokines, which are inflammatory cell products, to provide treatment and defense. Allows you to However, this action can also cause detrimental damage to adjacent tissue cells and non-cellular components. As EK, under appropriate conditions, after the initial state of inflammation has passed, normal function is restored, but if the irritant that stimulates the inflammation does not disappear or is made continuously, as a result, chronic inflammation occurs, leading to more serious tissue damage.

Representative skin problems related to inflammation include atopy and acne, and atopy is described as follows.

Atopy is characterized by severe itching.If the skin is irritated, the itching becomes more severe, and if you scratch more and more, the wound will be injured, resulting in bacterial infection (yellow staphylococcus, streptococcus, etc.), resulting in inflammation. One of the important factors in the problem of atopy is the secondary infection and superantigen toxin that occurs at this time. 80-90% of patients with Etopy have infections of Staphilococcus aureus and Streptococcus pyogenes on the skin, where anti-staphilococcus IgE is produced, thereby stimulating the T-cell response. It is also triggered by toxins and exacerbated. Staphylococcus aureus superantigen toxin selectively stimulates T lymphocytes through antigen-presenting cells in Langerhans cells to produce interleukin-1 and TNF-alpha, and various intracellular cytokines in T-cells stimulated by them It secretes a toxin called, causing inflammation. That is, the superantigen toxin promotes the secretion of interleukin-4 and interroutin-5, inhibits the secretion of IFN-gamma, and increases the number of CLA, thereby promoting the production of IgE.

Second, patients infected with Staphylococcus aureus secrete a substance called histamine from basophils by IgE antibodies against staphylococcal toxins, which worsens atopic dermatitis.

Therefore, the use of moisturizers is often recommended to prevent skin dryness, and secondary infection and the control of superantigenic factors resulting therefrom are very important. In other words, atopy sees a genetic defect as a basic etiology, but it is promoted by various external acquired factors.

Atopy-induced worsening factors include genetic factors, sensitive and dry skin, metabolic factors, excessive production of IgE, dysregulation of the autonomic nervous system, psychological factors, and stimulating factors.

As such, atopy is a disease whose mechanism is by no means simple. From an immunological point of view, it has a problem of various patterns, not one immunological problem. For example, the problem of gamma interferon, which suppresses excessive production of IgE, occurs because its production is less than the average value, but it is also caused by less than interleukin-4, which acts in the opposite direction, and the production process is problematic, resulting in the production of complete interferon. It may occur, and even if it is normal in terms of quantity or quality, sensitivity to interferon stimulation may decrease.

The key substance in these various inflammatory processes is arachidonic acid, which is converted from cell membrane lipids by an enzyme called phospholipase A2 (PLA2) activated by stimulation, and arachidonic acid is again cyclooxygenase. , Hereinafter referred to as'COX') or lipoxygenase, which is metabolized to prostaglandins and leulotrienes, which are inflammatory mediators, through two pathways, mediating various inflammatory reactions (Thomas, R. , Rev. Phsiol. Bioche. Pharmacol., 100, 1984)

Recently, research on a method to reduce the production of skin irritation and inflammation is actively progressing, and the results of the studies so far have shown that tissues or plasma are stimulated by nerve peptides such as substance P (substance P) secreted from nerve cells. Various cytokines, prostaglandin E2 (prostaglandin E2, hereinafter referred to as'PGE2'), etc., formed by kinins, such as bradykinin activated in, are involved in edema formation and vasodilation, causing inflammation and pain. It is known to be. Accordingly, as anti-inflammatory agents, neuropeptide antagonists, bradykinin antagonists, COX inhibitors, etc., including substance P, have been suggested, but anti-inflammatory agents known so far have most problems in terms of safety for the skin and stability when included in products. So its use is limited.

In order to solve the above problems, the present invention is to provide a cosmetic composition that can be safely used without side effects on the skin and has an anti-inflammatory effect, which is an effect of relieving skin troubles.

In order to solve the above technical problem, the present invention improves itching or anti-inflammatory, characterized in that it contains an extract of a complex herbal medicine consisting of centella asiatica, oleander, zymophila root, lotus seed, dandelion berry and honeysuckle flower as an active ingredient. It provides a cosmetic composition having an effect.

The cosmetic composition of the present invention is safe for the skin and has excellent anti-inflammatory effect, which is a skin trouble relief effect.

Hereinafter, the present invention will be described in more detail.

The cosmetic composition having an anti-inflammatory effect of the present invention is characterized in that it comprises as an active ingredient a herbal compound extract obtained by extracting a mixture of centella asiatica, oleifera, limbo root, lotus seed, dandelion berry and honeysuckle flower with hot spring water.

The centella asiatica is an outpost, the ally is the seed of the burdock (Arctium lappa), the root of the celiac is the rootstock of the Anemarrhena asphodeloides, and the lotus seed is the seed of the lotus flower (Nelumbo nucifera), The fruit is the fruit of the Forsythia suspensa, and the honeysuckle flower is the flower of the honeysuckle (Lonicera japonica).

The medicinal material may be prepared as an extract according to a conventional method, and for example, it may be prepared by a conventional method of extracting with an organic solvent such as hot spring water, distilled water, anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms, and then filtering.

The herbal compound extract obtained by extracting the mixture of centella, wubangja, jimo root, lotus seed, sugar lily fruit and honeysuckle flower with hot spring water is preferably contained in the cosmetic composition of the present invention in an amount of 0.0001 to 20% by weight, and more preferably Is contained in 0.01 to 10% by weight. If the content is less than 0.0001% by weight, a distinct effect cannot be expected.

The formulation of a cosmetic composition having an anti-inflammatory effect containing as an active ingredient the herbal compound extract obtained by extracting the mixture of centella asiatica, rhizome, chinensis root, lotus seed, sugar forsythia fruit and honeysuckle flower of the present invention as an active ingredient is not particularly limited. No, it can be appropriately selected according to the purpose. Specifically, the cosmetic composition may be prepared in a formulation such as softening lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, gel, lotion, ointment, patch or spray.

Hereinafter, preferred examples are presented to aid in the understanding of the present invention, but the following examples are only illustrative of the present invention, and the scope of the present invention is not limited to the following examples.

[Example]

Examples 1 to 2

After mixing equal amounts of dried Centella asiatica, Rhizoma, Jimo root, lotus seed, Dangali berry, and honeysuckle flower, they were finely chopped. After adding distilled water and 500 ml of hot spring water per 100 g of the minced medicinal mixture, it was aged at room temperature for 7 days. Then, the extracts were sequentially filtered through a filter of 0.4 µm, 02 µm, and 0.1 µm to prepare a distilled water extract and a hot spring water extract of the medicinal mixture.

[Experimental Example]

Experimental Example 1. NO (nitric oxide) production inhibitory effect

In order to confirm the skin trouble alleviation function of the anti-inflammatory effect of the herbal mixture extract prepared in Examples 1 to 2, a NO generation inhibitory effect test was performed.

Macrophage RAW 264.7 cells were dispensed into a 24-well plate at 2 X 10 ⁵ cells/ml using DMEM medium supplemented with 10% FBS, treated with LPS (500 ng/ml), and an extract sample was prepared after 1 hour. Treated by concentration and cultured for 24 hours. The generated NO was measured in the form of _{NO 2} ^- dissolved in the cell culture solution using the Griess reagent. 100ul of cell culture solution and 100ul of Griess reagent were mixed and reacted in a 96-well plate for 20 minutes, and the absorbance was measured and quantified at 540nm to determine the NO production inhibitory effect of the sample, and the results are shown in Table 1 below.

sample Inhibition rate (%) Control - Example 1
(Distilled water extract) 100ppm 23 200ppm 32 1000ppm 57 2000ppm 75 Example 2
(Hot spring water extract) 100ppm 31 200ppm 37 1000ppm 69 2000ppm 87

As shown in Table 1, Examples 1 to 2 of the present invention generate very strong NO It showed an inhibitory effect, and it was found that the efficacy of the extract using hot spring water was more excellent than that of the extract using distilled water.

[Production Example]

Preparation Example 1 and Comparative Example 1

Using Example 2, an ointment for external use of the skin was prepared according to the formulation shown in Table 2 below.

Composition weight% Manufacturing Example 1 Comparative Example 1 Extract of Example 2
Diethyl sebacate
Abiding
Polyoxyethylene oleyl ether phosphate
Sodium benzoate
vaseline 2
8
5
6
Appropriate amount
To 100 -
8
5
6
Appropriate amount
To 100

Preparation Example 2 and Comparative Example 2

Using Example 2, a cream was prepared according to the formulation shown in Table 3 below.

Composition weight% Manufacturing Example 2 Comparative Example 2 Extract of Example 2
Stearic acid
Cetanol
Potassium hydroxide
glycerin
Propylene glycol
antiseptic
incense
Purified water 2
15
One
0.7
5
3
Appropriate amount
Appropriate amount
To 100 -
15
One
0.7
5
3
Appropriate amount
Appropriate amount
To 100

Preparation Example 3 and Comparative Example 3

Using Example 2, a flexible lotion was prepared according to the formulation shown in Table 4 below.

Composition weight% Manufacturing Example 3 Comparative Example 3 Extract of Example 2
ethanol
Polylaurate polyoxyethylene sorbitan
Methyl paraoxybenzoate
glycerin
13-butylene glycol
incense
Pigment
Purified water 2
10
One
0.2
5
6
Appropriate amount
Appropriate amount
To 100 -
10
One
0.2
5
6
Appropriate amount
Appropriate amount
To 100

Preparation Example 4 and Comparative Example 4

Using Example 2, nutrient cosmetic water was prepared according to the formulation shown in Table 5 below.

Composition weight% Manufacturing Example 4 Comparative Example 4 Extract of Example 2
vaseline
Sorbitan sesquioleate
Polyoxyethyleneoleylethyl
Methyl paraoxybenzoate
Propylene glycol
ethanol
Carboxyvinyl polymer
Potassium hydroxide
Pigment
incense
Purified water 2
2
One
One
Appropriate amount
5
3
18
0.1
Appropriate amount
Appropriate amount
To 100 -
2
One
One
Appropriate amount
5
3
18
0.1
Appropriate amount
Appropriate amount
To 100

[Experimental Example]

Experimental Example 2. Itching and atopic dermatitis improvement effect experiment

Among the patients diagnosed with atopic dermatitis, about 20 patients complaining of severe itching were selected, and the cosmetics prepared according to Preparation Example 2 and Comparative Example 2 were applied twice a day (4 weeks) to the affected areas of both arms. The degree of itching and the degree of atopic dermatitis in Preparation Example and Comparative Example were judged by examination and visual inspection, and the degree of improvement in itching and atopic dermatitis in Preparation Example compared to Comparative Example was evaluated in three stages of distinct effect, effect, and no difference, and the result It is shown in Table 10 below.

Test Items Distinct effect (persons) Effective (persons) No difference (persons) Itching improvement 9 11 0 Atopic dermatitis improvement 4 15 2

As can be seen from the results of Table 6, the cosmetic prepared according to Preparation Example 2 was very excellent in improving itchiness and atopic dermatitis among 20 test subjects, and did not show any side effects in the skin, and was safe and very effective. It can be seen that it is a cosmetic for improving itchiness and atopic dermatitis.

Claims (5)

A cosmetic composition having an itching improvement or anti-inflammatory effect, characterized in that it contains as an active ingredient an extract of a complex herbal medicine consisting of Centella asiatica, oleifera, chinensis root, lotus seed, sugar lily fruit and honeysuckle The method of claim 1,
Centella asiatica: zymophila: roots: lotus seed: sugar lily fruit: honeysuckle flower, the weight ratio of 1: 0.1 ∼ 10: 0.1 ∼ 10: 0.1 ∼ 10 The method according to claim 1 or 2,
Cosmetic composition, characterized in that the itching is scalp itching or atopic dermatitis The method according to claim 1 or 2,
The extract is a cosmetic composition, characterized in that it is extracted with a solvent selected from the group consisting of purified water, hot spring water, C1 to C4 lower alcohol, or a mixture thereof The cosmetic composition of claim 1, wherein the content of the active ingredient is 0.00001 to 20% by weight based on the total weight of the composition.