KR20210046519A - Animal feed using probiotics and its manufacturing method - Google Patents

Animal feed using probiotics and its manufacturing method Download PDF

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KR20210046519A
KR20210046519A KR1020200028854A KR20200028854A KR20210046519A KR 20210046519 A KR20210046519 A KR 20210046519A KR 1020200028854 A KR1020200028854 A KR 1020200028854A KR 20200028854 A KR20200028854 A KR 20200028854A KR 20210046519 A KR20210046519 A KR 20210046519A
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probiotics
animal feed
mixing
spore
culturing
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KR102393430B1 (en
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정형학
주종원
박찬의
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(주)에이티바이오
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The present invention relates to an animal feed using probiotics and a method for manufacturing the same, comprising: a culturing step (S1a), an S.C. culturing step (S1b) and a base mixing step (S1c); a mixing step (S2); a production step (S3); a cutting step (S4); a confirmation step (St); and a packaging step (S5). By minimizing the number of live bacteria that are killed by heat during animal feed production, when an animal consumes feed, many probiotics enter the body and multiply, making the intestinal environment acidic and inhibiting the reproduction of harmful bacteria, thereby maintaining a healthy intestinal environment. In addition, the present invention relates to an animal feed using probiotics for improving the immune system and a method for manufacturing the same.

Description

프로바이오틱스를 활용한 사료 및 그 제조방법{Animal feed using probiotics and its manufacturing method}Feed using probiotics and its manufacturing method {Animal feed using probiotics and its manufacturing method}

본 발명은 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 있어서, The present invention in the animal feed using probiotics and a method for manufacturing the same,

동물이 포자 형성균인 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 1 및 Saccharomyces Cerevisiae와 같은 프로바이오틱스를 포함한 사료를 섭취하였을 때, 장에 도달하여 장 점막에 생육하며 장내 환경을 산성으로 만들어, 장내 유입된 유해균의 생육을 억제할 수 있어, 장내 환경을 건강하게 유지할 수 있는 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 관한 것이다. When animals consume feed containing spore-forming bacteria Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis or saccharomyces boulardii, and probiotics such as Saccharomyces Cerevisiae, they reach the intestine and grow into the intestinal mucosa and grow into the intestinal mucosa. , Animal feed using probiotics that can suppress the growth of harmful bacteria introduced into the intestine, and maintain a healthy intestinal environment, and a method of manufacturing the same.

프로바이오틱스 유익한 유산균의 증식과 이로 인한 장내 환경을 산성으로 만들어 유입된 유해균의 생육을 억제하고 또한 이로 인하여, 원활한 배변활동을 도모한다. 일반적으로 유산균이 프로바이오틱스로 많이 사용되고 있으나 열이나 산성에 취약하여 인체에 유산균이 유입되었을 때는 대부분의 프로바이오틱스는 사멸한다는 문제점이 있었다. 사멸된 프로바이오틱스 또한, 체내에서 유효물질을 분비할 수 있으나 사균보다는 생균이 장내에서 보다 원활하게 장내환경을 숙주에 유익하게 변화시키거나 유지할 수 있게 한다. 이와 관련하여 출원번호 제10-2015-0098886호 알로에추출물을 이용한 돼지사료 첨가제 제조장치에서는 선별된 알로에를 세척하는 세척기; 상기 세척기에 의해 세척된 알로에를 파쇄하는 파쇄기; 파쇄된 알로에를 감압추출하는 감압추출장치;상기 감압추출장치로부터 얻어진 알로에 추출물에 정제수와 프로바이오틱스균를 혼합하여 교반하는 교반기; 상기 교반기를 거친 교반물을 배양하여 돼지사료 첨가제를 완성하는 배양기;를 포함하여 구성되는 것을 특징으로 하는 알로에추출물을 이용한 돼지사료 첨가제 제조장치를 제안하였으나 프로바이오틱스를 혼합하고 있을 뿐, 상기 프로바이오틱스가 사료에 혼합되어 제조될 때, 사료의 제조공정 시 열에 의하여 유산균과 같은 프로바이오틱스는 사균이 된다는 문제점이 있었다. Probiotics Proliferation of beneficial lactic acid bacteria and the resulting intestinal environment are made acidic to inhibit the growth of introduced harmful bacteria, and thereby promote smooth bowel movements. In general, lactic acid bacteria are widely used as probiotics, but there is a problem that most probiotics die when lactic acid bacteria are introduced into the human body because they are vulnerable to heat or acid. Killed probiotics can also secrete effective substances in the body, but live bacteria rather than dead bacteria can change or maintain the intestinal environment beneficially to the host more smoothly in the intestine. In this regard, in the pig feed additive manufacturing apparatus using Application No. 10-2015-0098886 Aloe extract, a washing machine for washing the selected aloe; A crusher for crushing the aloe washed by the washing machine; A vacuum extraction device for extracting the crushed aloe under reduced pressure; A stirrer for mixing and stirring purified water and probiotic bacteria in the aloe extract obtained from the vacuum extraction device; A pig feed additive manufacturing apparatus using aloe extract, characterized in that it comprises: an incubator for culturing the agitated material through the stirrer to complete the pig feed additive, but only mixing probiotics, the probiotics are added to the feed. When prepared by mixing, there is a problem that probiotics such as lactic acid bacteria become dead by heat during the manufacturing process of feed.

상기 문제점을 개선하기 위하여, 본 발명은 포자 형성균인 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 1 및 Saccharomyces Cerevisiae를 이용하여 동물이 사료 섭취 시 장내 유입되는 생존 프로바이오틱스의 개체수를 확보하기 위한 프로바이오틱스를 활용한 동물 사료 및 그 제조방법을 제공하고자 한다. In order to improve the above problem, the present invention uses one of the spore-forming bacteria Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis or saccharomyces boulardii, and Saccharomyces Cerevisiae to secure the number of viable probiotics introduced into the intestine when animals feed intake. It is intended to provide an animal feed using probiotics and a method of manufacturing the same.

본 발명은 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 관한 것으로써, 사료 섭취시 장내 유입되는 생존 프로바이오틱스의 개체수를 확보하기 위한 프로바이오틱스를 활용한 동물 사료 및 그 제조방법을 제공하고자 한다. The present invention relates to an animal feed using probiotics and a method for manufacturing the same, and an object of the present invention is to provide an animal feed using probiotics and a method for manufacturing the same for securing the number of surviving probiotics introduced into the intestine when feed is consumed.

상기 과제를 해결하기 위하여, In order to solve the above problem,

본 발명은 배양단계(S1a), S.C.배양단계(S1b) 및 베이스혼합단계(S1c)와; 혼합단계(S2)와; 생산단계(S3)와; 절단단계(S4)와; 확인단계(St)와; 포장단계(S5)로; 구성되어 있어, 상기 배양단계에서는 포자 형성균인 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 1을 배양하고, S.C. 배양단계에서는 Saccharomyces cerevisiae를 배양한 후 베이스혼합단계(S1c)에서는 전분, 셀룰로오스, 생선살, 동물성원료가수분해물, 보존제, 연어유, 글리세린, 정제 포도당 및 정제수를 혼합한 후, 상기 배양단계(S1a) 및 S.C.배양단계(S1b)에서 생산된 포자형성균 및 Saccharomyces cerevisiae을 혼합단계(S2)에서 혼합하여 최종혼합물을 제조하고, 생산단계(S3)에서는 상기 최종혼합물을 60~130˚C에서 압출장치를 이용하여 압출혼합물을 생산하고 절단단계(S4)에서는 일정크기로 절단하고 확인단계(St)에서는 일정크기로 절단된 사료에서 생균 개체수를 확인한 후 포장하는 포장단계(S5)로; 구성된 프로바이오틱스를 활용한 동물 사료 및 그 제조방법을 제공함으로써 해결된다.The present invention includes a culture step (S1a), a S.C. culture step (S1b) and a base mixing step (S1c); Mixing step (S2) and; A production step (S3); Cutting step (S4) and; A confirmation step (St); To the packaging step (S5); In the culturing step, one of the spore-forming bacteria Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis, or saccharomyces boulardii is cultured, and S.C. In the culturing step, after culturing Saccharomyces cerevisiae, in the base mixing step (S1c), starch, cellulose, fish meat, hydrolyzate of animal raw materials, preservatives, salmon oil, glycerin, purified glucose and purified water are mixed, and the culturing step (S1a) And the spore-forming bacteria and Saccharomyces cerevisiae produced in the SC culture step (S1b) are mixed in the mixing step (S2) to prepare a final mixture, and in the production step (S3), the final mixture is extruded at 60-130°C. In the packaging step (S5) of producing an extruded mixture using, cutting into a certain size in the cutting step (S4), and checking the number of viable bacteria in the feed cut into a certain size in the confirmation step (St) and packing; It is solved by providing an animal feed using the configured probiotics and a method of manufacturing the same.

본 발명은 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 관한 것으로써, 사료 제조시 열에 의하여 사멸되는 생균의 개체수를 최소화하여, 동물이 사료를 섭취할 때, 최대한 많은 개체수의 프로바이오틱스가 체내로 유입되어, 증식하면서 장내 환경을 산성으로 만들고, 유해균의 번식을 억제할 수 있어, 장내 환경을 건강하게 유지할 수 있으며, 또한, 면역시스템을 향상시킬 수 있다는 효과가 있다. The present invention relates to an animal feed using probiotics and a method for manufacturing the same.By minimizing the number of live bacteria killed by heat during feed manufacturing, when the animal ingests the feed, as many probiotics as possible are introduced into the body. , It has the effect of making the intestinal environment acidic while proliferating, inhibiting the reproduction of harmful bacteria, keeping the intestinal environment healthy, and improving the immune system.

도 1은 프로바이오틱스를 활용한 동물 사료 제조방법에 대한 흐름도이다.1 is a flow chart of a method for producing animal feed using probiotics.

이하, 첨부된 도면을 참고하여 보다 상세하게 설명하고자 한다.Hereinafter, it will be described in more detail with reference to the accompanying drawings.

이에 앞서, 본 명세서 및 청구범위에 사용되는 용어나 단어는 통상적이거나 사전적인 의미로 한정해서 해석되어서는 아니 되며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위하여 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다.Prior to this, terms or words used in the present specification and claims should not be construed as being limited to their usual or dictionary meanings, and the inventors appropriately explain the concept of terms in order to explain their own invention in the best way. Based on the principle that it can be defined, it should be interpreted as a meaning and concept consistent with the technical idea of the present invention.

따라서, 본 명세서에 기재된 실시 예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시 예에 불과할 뿐이고, 본 발명의 기술적 사상을 모두 대변하는 것은 아니므로, 본 출원 시점에 있어서 이들은 대체할 수 있는 다양한 균등물과 변형 예들이 있을 수 있음을 이해하여야 한다.Therefore, the embodiments described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all the technical ideas of the present invention. It should be understood that there may be various equivalents and variations.

본 발명은 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 관한 것으로써,The present invention relates to an animal feed using probiotics and a method for manufacturing the same,

크게, 배양단계(S1a), S.C.배양단계(S1b) 및 베이스혼합단계(S1c)와; 혼합단계(S2)와; 생산단계(S3)와; 절단단계(S4)와; 확인단계(St)와; 포장단계(S5)로; 구성되어 있는 프로바이오틱스를 활용한 동물 사료 및 그 제조방법에 관한 것이다. Largely, a culture step (S1a), a S.C. culture step (S1b) and a base mixing step (S1c); Mixing step (S2) and; A production step (S3); Cutting step (S4) and; A confirmation step (St); To the packaging step (S5); It relates to animal feed and a method of manufacturing the same using the constituted probiotics.

보다 구체적으로는, More specifically,

상기 배양단계(S1a)에서는 pH 5.0~7.5 및 30~55℃의 환경에서 포자형성균을 배양하는 단계이며, 포자형성균의 특성상 130℃의 고온에 환경 속에서도 생존이 가능하여, 사료 제조시에도 쉽게 사멸되지 아니하고, 대사 과정 중에 유효물질이 생성되어 장내에서 유익한 작용할 수 있다. The culturing step (S1a) is a step of culturing spore-forming bacteria in an environment of pH 5.0-7.5 and 30-55°C, and due to the nature of spore-forming bacteria, it is possible to survive in the environment at a high temperature of 130°C. It is not killed, and effective substances are produced during metabolic processes, which can have a beneficial effect in the intestine.

상기 포자형성균에는 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 1이 될 수 있다.The spore-forming bacteria may be one of Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis, or saccharomyces boulardii.

상기 S.C.배양단계(S1b)에서는 25~50℃의 환경에서 Saccharomyces cerevisiae를 배양하는 단계이며, Saccharomyces cerevisiae는 산성 환경과 90℃의 고온의 환경 속에서도 생존이 가능하여, 사료 제조 시에도 쉽게 사멸되지 아니하고, 대사 과정 중에 유효물질이 생성되어 장내에서 유익한 작용할 수 있으며,The SC culture step (S1b) is a step of culturing Saccharomyces cerevisiae in an environment of 25 to 50°C, and Saccharomyces cerevisiae can survive in an acidic environment and a high temperature environment of 90°C, so it is not easily killed even during feed production, Effective substances are produced during the metabolic process and can have a beneficial effect in the intestine,

상기 베이스혼합단계(S1c)에서는 전분, 셀룰로오스, 생선살, 동물성원료가수분해물, 보존제, 연어유, 글리세린, 정제 포도당 및 정제수를 혼합하는데, 전분 40~45g 기준, 셀룰로오스 15~19g, 생선살 15~19g, 동물성원료가수분해물 5~7g, 보존제 1~3g, 연어유 1~2g, 글리세린 5~7g, 정제 포도당 2~3.5g 및 정제수 2~4g을 혼합하여 베이스혼합물을 제조한다. In the base mixing step (S1c), starch, cellulose, fish meat, hydrolyzate of animal raw materials, preservatives, salmon oil, glycerin, refined glucose and purified water are mixed, based on starch 40-45g, cellulose 15-19g, fish meat 15- 19g, animal raw material hydrolyzate 5-7g, preservative 1~3g, salmon oil 1~2g, glycerin 5~7g, refined glucose 2~3.5g, and purified water 2~4g are mixed to prepare a base mixture.

상기 혼합단계(S2)는 베이스혼합단계(S1c)를 통하여 생성된 베이스혼합물의 전분 40~45g기준 배양단계(S1a)에서 배양된 포자형성균인 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 어느 하나의 0.8~1.0g과 S.C.배양단계(S1b)에서 배양된 Saccharomyces cerevisiae의 0.8~1.0g를 혼합하여 최종혼합물을 제조하는 단계이며, The mixing step (S2) is Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis or saccharomyces boulardii, which are spore-forming bacteria cultured in the culturing step (S1a) based on 40-45 g of starch of the base mixture produced through the base mixing step (S1c). It is a step of preparing a final mixture by mixing 0.8 to 1.0 g of any one and 0.8 to 1.0 g of Saccharomyces cerevisiae cultured in the SC culture step (S1b),

상기 생산단계(S3)에서는 상기 최종혼합물을 섭씨 60~130도에서 압출장치를 이용하여 압출혼합물을 생산하는 단계이며, 배양단계(S1a)에서 배양된 포자형성균과 S.C.배양단계(S1b)에서 배양된 Saccharomyces cerevisiae의 생존율을 높이기 위하여 섭씨 60~130도에서 압출혼합물을 생산하게 된다.In the production step (S3), the final mixture is produced using an extrusion device at 60 to 130 degrees Celsius, and the spore-forming bacteria cultivated in the culturing step (S1a) and the SC culture step (S1b) are cultivated. In order to increase the survival rate of Saccharomyces cerevisiae, the extruded mixture is produced at 60-130 degrees Celsius.

상기 절단단계(S4) 는 상기 생산단계에서 생산된 압출혼합물을 8~12CM로 절단하여 절단사료를 생산하는 단계이며, The cutting step (S4) is a step of cutting the extruded mixture produced in the production step into 8 to 12 CM to produce cut feed,

상기 확인단계(St)는 절단단계에서 생산된 절단사료에 포함된 포자형성균 및 Saccharomyces cerevisiae의 생산단계에서 생존한 개체 수를 확인하는 단계이며, The confirmation step (St) is a step of confirming the number of spore-forming bacteria contained in the cut feed produced in the cutting step and the number of individuals surviving in the production step of Saccharomyces cerevisiae,

상기 포장단계(S5)는 절단사료를 포장하는 단계이다. The packaging step (S5) is a step of packaging the cut feed.

실시 예로는 Examples are

배양단계(S1a)에서 배양된 포자형성균인 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 어느 하나와 S.C.배양단계(S1b)에서 배양된 Saccharomyces cerevisiae를 베이스혼합단계(S1c)에 혼합된 베이스혼합물을 혼합하여, 혼합단계(S2)에서 최종혼합물을 제조한 후, 생산단계(S3)에서는 압출장치를 이용하여 압출혼합물을 제조하고 절단단계(S4)에 압출혼합물을 일정크기로 절단하여 절단사료를 제조한 후 확인단계(St)에서 상기 절단사료 기준 포자형성균 및 Saccharomyces cerevisiae 생균 개체수를 1x10^6 CFU/g 이상인지 확인하여 기준치 이상이면 포장단계(S5)를 거쳐 포장하게 된다. Mixing the base mixing step (S1c) with any one of the spore-forming bacteria cultured in the cultivation step (S1a), Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis, or saccharomyces boulardii, and Saccharomyces cerevisiae cultivated in the SC cultivation step (S1b). After mixing the base mixture to prepare the final mixture in the mixing step (S2), in the production step (S3), the extruded mixture was prepared using an extrusion device, and the extruded mixture was cut into a certain size in the cutting step (S4) and cut. After preparing the feed, in the confirmation step (St), the number of spore-forming bacteria and Saccharomyces cerevisiae live bacteria based on the cut feed is checked to be 1x10^6 CFU/g or more, and if it is more than the standard value, packaging step (S5) is performed.

이상과 같이 본 발명에서는 구체적인 구성 요소 등과 같은 특정 사항들과 한정된 실시 예 및 도면에 의해 설명되었으나 이는 본 발명의 보다 전반적인 이해를 돕기 위해서 제공된 것일 뿐 본 발명은 상기의 실시 예에 한정되는 것은 아니며, 본 발명이 속하는 분야에서 통상적인 지식을 가진 자라면 이러한 기재로부터 다양한 수정 및 변형이 가능하다.As described above, in the present invention, specific matters such as specific components, etc., and limited embodiments and drawings have been described, but this is provided to help a more general understanding of the present invention, and the present invention is not limited to the above embodiments, Anyone of ordinary skill in the field to which the present invention belongs can make various modifications and variations from these descriptions.

따라서, 본 발명의 사상은 설명된 실시 예에 한정되어서는 아니 되며, 후술하는 특허청구범위뿐만 아니라 이 특허청구범위와 균등하거나 등가적 변형이 있는 모든 것들은 본 발명의 범주에 속한다고 할 것이다.Accordingly, the spirit of the present invention is not limited to the described embodiments, and all things equivalent or equivalent to the claims as well as the claims to be described later belong to the scope of the present invention.

Claims (7)

프로바이오틱스를 활용한 동물 사료 제조방법에 있어서,
pH 5.0~7.0 및 30~55℃의 환경에서 포자 형성균을 배양하는 배양단계(S1a)와;
30~55℃의 환경에서 Saccharomyces cerevisiae를 배양하는 S.C.배양단계(S1b)와;
전분, 셀룰로오스, 생선살, 동물성원료가수분해물, 보존제, 연어유, 글리세린, 정제 포도당 및 정제수를 혼합하여 베이스혼합물을 제조하는 베이스혼합단계(S1c)와;
베이스혼합단계(S1c)를 통하여 생성된 베이스혼합물에 배양단계(S1a)에서 배양된 포자형성균과 S.C.배양단계(S1b)에서 배양된 Saccharomyces cerevisiae를 혼합하여 최종혼합물을 제조하는 혼합단계(S2)와;
상기 최종혼합물을 섭씨60~130도에서 압출장치를 이용하여 압출혼합물을 생산하는 생산단계(S3)로; 구성되어 있는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료 제조방법.
In the animal feed manufacturing method using probiotics,
a culture step (S1a) of culturing spore-forming bacteria in an environment of pH 5.0 to 7.0 and 30 to 55°C;
SC culture step (S1b) of culturing Saccharomyces cerevisiae in an environment of 30 ~ 55 ℃;
A base mixing step (S1c) of preparing a base mixture by mixing starch, cellulose, fish meat, hydrolyzate of animal raw materials, preservatives, salmon oil, glycerin, refined glucose and purified water;
The mixing step (S2) of preparing a final mixture by mixing the spore-forming bacteria cultured in the culturing step (S1a) and the Saccharomyces cerevisiae cultivated in the SC culturing step (S1b) in the base mixture produced through the base mixing step (S1c). ;
The final mixture to a production step (S3) of producing an extruded mixture using an extrusion device at 60 to 130 degrees Celsius; Animal feed manufacturing method using probiotics, characterized in that consisting of.
 제 1항에 있어서,
상기 생산단계(S3)에서 생산된 압출혼합물을 8~12CM로 절단하여 절단사료를 생산하는 절단단계(S4)를; 더 포함하는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료 제조방법.
The method of claim 1,
A cutting step (S4) of cutting the extruded mixture produced in the production step (S3) into 8 to 12CM to produce cut feed; Animal feed manufacturing method using probiotics, characterized in that it further comprises.
 제 2항에 있어서,
절단단계(S4)에서 생산된 절단사료에 포함된 포자형성균 및 Saccharomyces cerevisiae가 생산단계에서 생존한 개체 수를 확인하는 확인단계(St)를; 더 포함하는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료 제조방법.
The method of claim 2,
A confirmation step (St) of confirming the number of spore-forming bacteria and Saccharomyces cerevisiae surviving in the production step contained in the cut feed produced in the cutting step (S4); Animal feed manufacturing method using probiotics, characterized in that it further comprises.
 제 1항에 있어서,
포자 형성균은 Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis 또는 saccharomyces boulardii 중 어느 하나가 될 수 있는 것을 특징으로 하는 프로바이오틱스를 활용한 동물사료 제조방법.
The method of claim 1,
The spore-forming bacteria can be any one of Bacillus coagulans, Bacillus subtills, Bacillus cereus, Bacillus licheniformis, or saccharomyces boulardii.
 제 1항에 있어서,
상기 베이스혼합단계(S1c)에서는 전분 40~45g 기준, 셀룰로오스 15~19g, 생선살 15~19g, 동물성원료가수분해물 5~7g, 보존제 1~3g, 연어유 1~2g, 글리세린 5~7g, 정제 포도당 2~3.5g 및 정제수 2~4g을 혼합하여 베이스혼합물을 생성하는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료 제조방법.
The method of claim 1,
In the base mixing step (S1c), based on 40 to 45 g of starch, 15 to 19 g of cellulose, 15 to 19 g of fish meat, 5 to 7 g of hydrolyzed animal raw materials, 1 to 3 g of preservative, 1 to 2 g of salmon oil, 5 to 7 g of glycerin, tablets Animal feed manufacturing method using probiotics, characterized in that the base mixture is produced by mixing 2 to 3.5 g of glucose and 2 to 4 g of purified water.
 제 1항에 있어서,
혼합단계(S2)는 상기 전분 40~45g 기준 포자형성균과 Saccharomyces cerevisiae는 각각 0.8~1.0g이 혼합하여 최종혼합물을 제조하는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료 제조방법.
The method of claim 1,
The mixing step (S2) is a method for producing animal feed using probiotics, characterized in that 0.8 to 1.0 g of spore-forming bacteria and Saccharomyces cerevisiae are mixed with each of the starch based on 40 to 45 g to prepare a final mixture.
 제 1항 내지 6항 중 어느 한 항의 방법을 이용하여 제조되는 것을 특징으로 하는 프로바이오틱스를 활용한 동물 사료.
Animal feed utilizing probiotics, characterized in that it is produced using the method of any one of claims 1 to 6.
KR1020200028854A 2019-10-18 2020-03-09 Animal feed using probiotics and its manufacturing method KR102393430B1 (en)

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JP2012502920A (en) * 2008-09-17 2012-02-02 アグラクエスト インコーポレイテッド How to use Bacillus subtilis strains to improve animal health
KR20100080266A (en) * 2008-12-29 2010-07-08 주식회사 대호 Probiotics for feed additives
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