KR20210042612A - Method for improving germination rate and quality of Upright Ladybell seedling using artificial culture medium - Google Patents

Abstract

The present invention relates to a method for improving the germination rate and seedling quality of small seedlings using an artificial medium. According to the present invention, by selecting an appropriate artificial medium for growth improvement in the production of small seedlings in a closed plant production system, it is possible to not only produce fine seedlings but also to reduce labor and increase productivity through uniform growth of seedlings.

Description

Method for improving germination rate and quality of Upright Ladybell seedling using artificial culture medium}

The present invention relates to a method of improving the germination rate and quality of seedlings of seedlings using artificial medium.

In recent years, as a stable supply of vegetable seedlings is required for year-round production of facility vegetables, process seedling technology capable of mass-producing seedlings has become generalized, making it possible to produce uniform and standardized seedlings. In recent years, unlike bedrock for seedling, the use of artificial medium of constant shape such as rock wool allows fruit and vegetable seedlings to be easily transplanted and planted for a short period of time, and thus the availability of artificial medium in the seedling industry has been confirmed, and is being developed at home and abroad. .

Adenophora triphylla var. japonica Hara is a perennial herbaceous native plant belonging to the Campanulaceae family, and young shoots in early spring are known as ‘Tokju’ as a representative wild vegetable used for food. It is used as a herbal medicine and food. The root is known to have a similar medicinal effect to ginseng because it is called'sasam', and it is used as medicinal materials such as geodam, jinhae, geonwi, and tonic in oriental medicine.

Zandae grows wild at the foot of sunny mountains in the mountains of Korea. There are many kinds of similar species, such as round basin, wide basin, dangjandae, and island basin. The stalk is propagated as a seed, and there are direct cultivation, transplant cultivation, and flower bed cultivation to see flowers. Since the stalks do not like transplantation, it is preferable to mainly cultivate them directly, but there are cases where seedlings are cultivated for high yield. Some physiological studies, such as germination characteristics, have been conducted on the stalks, but research on cultivation techniques is insufficient.

On the other hand, Korean Patent No. 1447118 discloses'a solid medium composition for cultivating artificial seed potatoes and a one-step artificial seed potato cultivation method using the same', and Korean Patent No. 1792149 discloses a'method for producing artificial medium for crop cultivation'. Although disclosed, there is no description of a method for improving the germination rate and seedling quality of stalk seedlings using the artificial medium of the present invention.

The present invention was derived from the above requirements, and the inventors of the present invention in order to find out a method for producing high-quality seedlings through the selection of an appropriate artificial medium for seedlings in a closed plant production system, urethane sponge, LC (LC grow foam), RW (Rockwool) and TP (Terra Plug) medium, respectively, and seedling for 72 days, the germination rate was found to be the highest in TP medium among 4 types of artificial medium. The present invention was completed by confirming that the growth indices of stalk seedlings such as middle and maximum root diameter, total root length, root surface area, root volume, and root tip number are also the best in the TP medium.

In order to solve the above problems, the present invention is to, Adenophora triphylla seedlings germination and growth, comprising the step of raising seedling with planting the Adenophora triphylla (Adenophora triphylla var. Japonica Hara) in artificial media TB plug (terra plug) within the closed plant production system Provides a way to improve.

In addition, the present invention includes the step of sowing seeds of Adenophora triphylla var. japonica Hara in a terra plug artificial medium and seeding them in a hydroponic method in a closed plant production system, with improved germination rate and growth. It provides a method of cultivating a seedling of a seedling, and a seedling of a seedling having improved germination rate and growth cultivated by the above-described cultivation method.

In addition, the present invention provides a medium composition for improving the germination rate and growth of seedlings in a closed type plant production system containing terra plug artificial medium as an active ingredient.

In the present invention, by selecting an appropriate artificial medium for improving the growth during the production of seedlings in the closed plant production system, it is possible to not only produce excellent seedlings but also reduce labor and increase productivity through uniform growth of the seedlings.

1 is a view of four types of artificial media used in the experiment.
2 is a graph showing the spectral distribution in the closed plant production system used in the experiment.
Figure 3 shows the germination rate of twigs grown under various artificial mediums.
Figure 4 shows the number of leaves (A) and the leaf area (B) 72 days after sowing of the twigs grown under various artificial media.
5 shows the maximum root length (A), the maximum root diameter (B), the total root length (C), the root surface area (D), the root volume (E) and the number of root tips ( F) is shown.
6 is a view of growth on the 72nd day after sowing of stalks grown under various artificial mediums.
Fig. 7 shows the growth of roots at 72 days after sowing of twigs grown under various artificial media.

According to an aspect of the invention there is provided, Adenophora triphylla seedlings germination and which comprises raising seedling with planting the Adenophora triphylla (Adenophora triphylla var. Japonica Hara) to TB plug (terra plug) artificial media in a closed plant production system Provides a way to improve growth.

The'terra plug artificial medium' is a commercialized artificial medium for hydroponic cultivation, and unlike the top soil, it is an artificial medium having a constant shape. Conventional plant factories centered on the hydroponics cultivation of leafy vegetables such as lettuce, but recently, plant factories are also attempting to cultivate hydroponics for root vegetables. There is a trend of increasing use.

Since the physical properties (container capacity, weather rate, total void, and temporary weight) are different depending on the type of artificial medium, it is important to select and use an appropriate artificial medium suitable for the characteristics of the crop. The amount of water used for the container represents the moisture content of the medium, and if the medium can contain a large amount of nutrient moisture, the moisture content of the root zone does not decrease rapidly, thereby increasing the efficiency of using nutrient water and reducing the number of irrigation. Gravity water is discharged after the medium is trapped, creating a space for important oxygen to enter the root region, and the ratio of this space in the medium is called the meteorological rate. It is believed that if the weather rate is high, sufficient oxygen can be supplied to the root zone.

In the method for improving the germination rate and growth of seedlings according to an embodiment of the present invention, the light quality in the closed plant production system may be adjusted to a ratio of red light and blue light to 6 to 8: 2 to 4, preferably May be a light quality in which the ratio of red light and blue light is 7:3, but is not limited thereto. In a closed plant production system, a method of using a light emitting diode (LED) that emits a light wavelength in a specific region is used to control light quality. The plant cultivation method using LED consumes less power than incandescent lamps or fluorescent lamps, and is an efficient method for plant cultivation because it can selectively irradiate the wavelength of a specific desired region excluding unnecessary wavelength regions.

The present invention also includes the step of sowing seeds of Adenophora triphylla var. japonica Hara in a terra plug artificial medium in a closed plant production system and seeding in a hydroponic cultivation method for 70 to 74 days, germination rate And it provides a method of cultivating a seedling of seedlings with improved growth, and a seedling of seedlings with improved germination and growth rate cultivated by the cultivation method.

The cultivation method of seedlings of the present invention is specifically,

Seeding the seeds of the seedlings in the plug tray filled with the terra plug artificial medium;

In a sealed plant production system that maintains a temperature of 27 to 31°C and a relative humidity of 50 to 70% in the plug tray in which the seedlings are sown, the specificity and the memorization time are the same, and 130 to 170 μmol·m ^-2 · germinating for 13 to 17 days by irradiating red light with a luminosity in the range of s ^-1; And

^{Using a nutrient solution having an electrical conductivity of 0.7 to 1.3 dS·m -1} in a recirculating submerged hydroponic cultivation system for the germinated seedlings, a temperature of 23 to 27°C and a relative humidity of 50 to 70% are maintained. A step of seeding for 55 to 59 days by irradiating an LED light source whose memorization time is the same and the ratio of red light and blue light is adjusted to 6 to 8: 2 to 4 with a brightness in the range of ^{130 to 170 μmol·m -2} ·s ^-1 It may include, but is not limited thereto.

Seedlings grown by the cultivation method according to the present invention are plant length, leaf length, leaf width, leaf tree, crown diameter, above-ground and underground portions compared to those using urethane sponge, LC (LC grow foam) and RW (Rockwool) artificial medium. The fresh weight and dry weight of, the maximum root diameter, the total root length, the root surface area, the root volume, and the number of root tips may be increased, but are not limited thereto.

In addition, the present invention provides a medium composition for improving the germination rate and growth of seedlings in a closed type plant production system containing terra plug artificial medium as an active ingredient. Terra plug artificial medium and growth improvement are as described above.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Materials and methods

1. Plant data and growing conditions

Seeds of Adenophora triphylla var.japonica Hara were prepared in 5 artificial media (urethane sponges (US, Gafatec Co. Ltd., Hwaseong, Korea), RW (Rockwool, Item #: 11174197; Grodan Co.) on August 7, 2018. Ltd., The Netherlands) medium, LC (LC grow foam, code #: K-5810; Smithers-Oasis Korea) medium, TP (Terra Plug, code #: K-87-61128; Smithers-Oasis Korea) medium] It was sown in 128 trays each filled with and germinated for 15 days in a closed plant production system (C1200H3, FC Poibe Co. Ltd., Seoul, Republic of Korea). Growth conditions during germination are temperature 29±1℃, relative humidity 60±10%, photoperiod 12/12 (light/dark), and luminous intensity 150 μmol·m ^-2 ·s ^-1 PPFD (photosynthetic photon flux) under a red LED light source. density). Distilled water was supplied to the bottom irrigation during the germination of the seedlings.

^{The germinated seedlings were cultivated for 57 days using Hoagland (pH 5.8, EC 1.0 dS m -1} ) nutrient solution using a recirculation immersion method (DFT, Deep Flow Technique) hydroponic cultivation method. Growing environment is an LED (red:blue = 7:3) in which red light and blue light are mixed in 7: 3 respectively, temperature 25±1±℃, relative humidity 60±10%, photoperiod 12/12 (light/dark) under the light source , And luminosity of 180 μmol·m ^-2 ·s ^-1 PPFD.

2. Experimental design and growth survey items

From the 8th to the 15th day after sowing, the germination rate of the seedlings of each artificial medium was analyzed every day, and on the 72nd day after sowing, the height, leaf length, leaf width, crown diameter, number of leaves, leaf area, and live weight of each artificial medium were analyzed. And dry weight, maximum root length, maximum root diameter, total root length, root surface area, root volume, and root tip number were measured. In addition, WinRhizo Pro 2007a was used for root image analysis.

For statistical analysis, ANOVA was performed using a SAS program (SAS 9.4, SAS Institute Inc., Cary, NC, USA), and the comparison between means was performed using Duncan's multiple range test. The significance between each treatment was verified at the% significance level. The graph was shown using the SigmaPlot program (Sigma Plot 12.0, Systat Software Inc., San Jose, CA, USA).

Example 1. Germination rate and growth characteristics of larvae according to the type of artificial medium

The germination rate of twigs was highest in TP medium, followed by high in LC medium, and low in US and RW medium (FIG. 3). In addition, on the 72nd day after sowing, the plant height, leaf length, and leaf width of the seedlings were the longest in the TP medium and the shortest in the US medium. The diameter of the crown, which is the area where the stem and the root contact, was also confirmed to be the thickest in the TP medium (Table 1).

Plant length, leaf length, leaf width, and crown diameter of seedlings by artificial medium type Badge type Length (cm) Leaf length (cm) Leaf width (cm) Crown diameter (mm) US 3.97 c ^z 1.42 b 1.97 c 1.39 b LC 8.20 ab 2.36 a 4.30 b 2.54 b RW 7.48 b 2.13 ab 3.34 bc 1.87 b TP 10.51 a 2.74 a 6.07 a 4.97 a ^z Mean separation within columns by Tukey's multiple range test at P ≤ 0.05.

The live weight and dry weight of the above-ground and underground parts were found to be the heaviest in the TP medium with improved growth of stalk seedlings (Table 2).

Live weight and building weight of the above-ground and underground parts of the cultivated seedlings by type of artificial medium Badge type Live weight (g/plant) Building weight (g/plant) Shoot Root Shoot Root US 0.12 b ^z 0.13 b 0.018 b 0.014 b LC 0.62 b 0.72 ab 0.107 b 0.070 b RW 0.38 b 0.46 b 0.057 b 0.045 b TP 2.34 a 1.75 a 0.370 a 0.209 a ^z Mean separation within columns by Tukey's multiple range test at P ≤ 0.05.

The number of leaves of the stalk seedling was the most in the TP medium, followed by the large number in the LC medium, and the smallest in the US and RW medium (Fig. 4A). In addition, the leaf area was also the widest in the TP medium with many leaves (Fig. 4B). The maximum root length was the shortest in the US medium, and the maximum root diameter, total root length, and root surface area were highest in the TP medium (FIGS. 5A to 5D). The root volume was highest in the TP medium, and no significant difference was observed in the remaining medium treatment (FIG. 5E). The number of root tips was the highest in TP medium and significantly lowest in US medium (FIG. 5F). The above results were inferred that the TP medium, which is an organic medium, was advantageous for the growth of the underground part of the seedlings and promoted the development of the underground part, and accordingly, the growth of the above-ground part also increased. As a result, TP medium, an artificial medium, had an effect on the growth and development of Adenophora triphylla var. It was found that it can be used as a useful and practical technique to produce.

Claims (7)

TB plug in the closed plant production systems (terra plug) Adenophora triphylla on artificial media (Adenophora triphylla var. Japonica Hara) the method of planting and including the step of raising seedling, Adenophora triphylla improve the germination and growth of seedlings. The method of claim 1, wherein the light quality in the closed-type plant production system has a ratio of red light and blue light adjusted to 6-8:2-4. A method of cultivating seedlings with improved germination and growth, comprising the step of sowing seeds of Adenophora triphylla var.japonica Hara in a terra plug artificial medium and seeding them in a hydroponic method in a closed plant production system. The method of claim 3, wherein the light quality in the closed-type plant production system has a ratio of red light and blue light adjusted to 6-8:2-4. The method of claim 3, wherein the root length, leaf length, leaf width, leaf tree, crown diameter, fresh weight and dry weight of the above and underground parts, maximum root diameter, total root length, root surface area, root volume, and root tip number are increased. A method of cultivation of seedlings with improved growth. Seedlings with improved germination rate and growth cultivated by the cultivation method of any one of claims 3 to 5. Terra plug (terra plug) Medium composition for improving the germination rate and growth of seedlings in a closed type plant production system containing an artificial medium as an active ingredient.

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