KR20210005459A - Antimicrobial composition comprising the extract of flower bud of panax ginseng c. a. meyer - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising an extract of a flower bud of panax ginseng C.A. meyer as an effective ingredient, and more specifically, to a wide antimicrobial composition comprising an ethanol extract of a flower bud of panax ginseng C.A. meyer as an effective ingredient. As proven through the examples in the specification, the extract of a flower bud of panax ginseng C.A. meyer, which is an effective ingredient of the wide antimicrobial composition of the present invention, has excellent effects for antibacterial pharmaceutical composition, health functional food, cosmetic composition, animal feed composition, food, cosmetics or additives for animal feed, various detergents and various cleaning agents. In addition, the ginseng bud extract of the present invention has excellent thermal stability and there is no loss of activity even under acidic conditions of pH 2 and plasma, so that it can be easily processed into various forms such as liquid, cream, powder, pills, tablets, etc. Thus, it can be very usefully used in the pharmaceutical industry, food industry, livestock industry, health industry and cosmetic industry.

Description

An antibacterial composition containing ginseng bud extract as an active ingredient {ANTIMICROBIAL COMPOSITION COMPRISING THE EXTRACT OF FLOWER BUD OF PANAX GINSENG C. A. MEYER}

The present invention relates to an antibacterial composition containing an extract of ginseng bud (flower bud of Panax ginseng CA Meyer) as an active ingredient, and more particularly, to a broad-spectrum antibacterial composition containing an ethanol extract of ginseng bud as an active ingredient. will be.

Humans have used various antibacterial agents and preservatives to prevent invasion of pathogenic microorganisms and control food spoilage microorganisms. However, in the case of chemical antimicrobial agents and preservatives, they exhibit excellent antibacterial activity against various bacteria and fungi, but when used for a long time, many side effects such as redness due to allergy, vascular edema, bone marrow suppression, anemia, gastritis, and degenerative brain disease appear. do. In fact, the use of antibiotics with severe side effects such as chloramphenicol has been banned in the United States. Therefore, various studies on the development of natural antibacterial agents using natural products with long-term use experience while securing safety are in progress.

Meanwhile, according to the U.S. Center for Disease Control and Prevention, bloodstream infections in hospitals are one of the leading causes of death in the United States. In addition, infection of pathogenic bacteria and fungi through skin and soft tissues is recognized as a universal medical disease, and is understood to be mainly caused by trauma or surgery. Many pathogenic bacteria and fungi infections are treated differently depending on the severity of the infection, but successful treatment is possible with oral antimicrobial agents and topical cleansing. However, more severe or complex infections commonly occurring in patients with infections caused by underlying risk factors (e.g., vascular blood flow, diabetes) and/or difficult to treat or multi-resistant bacteria and fungi are venous Measures such as antimicrobial treatment and invasive surgical tissue removal may be necessary. For example, vancomycin, a glycopeptide-based antibiotic, has been successfully used against severe nosocomial infections caused by multi-drug-resistant Gram-positive bacteria, especially MRSA, coagulase-negative Staphylococcus and Enterococcus. However, it has been reported that the isolation of strains resistant to vancomycin is increasing recently, and it is true that treatment for severe infections caused by some of the pathogens is very limited with current antibiotics. Therefore, there is an increasing need to develop new antibiotics that are more effective against the increasing resistance bacteria.

On the other hand, Panax ginseng CA Meyer is a perennial herbaceous plant belonging to the genus Panax of Araliaceae. It has 2 to 5 side roots in the shape of a long, cylindrical or spindle shape, and the outer surface is pale yellowish brown. There are longitudinal wrinkles and fine root marks. At the root head, there is an outcrop to which the stem residues are attached. In oriental medicine, the root of ginseng is mainly used as a medicinal material, and it is known to boost vitality, physical weakness, boredom, fatigue, loss of appetite, vomiting, and diarrhea. Actually known pharmacological actions include cerebral cortex excitation and inhibition, equilibrium, anti-fatigue, anti-aging, immunity enhancement, heart contraction, gonadotropin, hyperglycemia suppression, protein synthesis promotion, homeostasis, anti-cancer, detoxification, etc. It has a scent, sweet taste, a little bitter, and a little warm in nature. Ginseng can be used not only for roots, but also for leaves, stems, fruits, and seeds. In particular, ginseng leaves are used for ginseng leaves, outcrops are used for ginseng, seeds are used for ginseng. It is also called as Ja (人蔘子), and it is all used as a valuable medicine in oriental medicine.

Ginseng has been intensively studied underground, but research on ginseng buds is insignificant. In Korea, ginseng produces one peduncle in summer, and at the end of it, 4 to 40 light green small flowers hang in an umbel, and flowers usually start to bloom from 3 to 4 years and bloom in mid-May. Ginseng flowers are disposed of without separate use, and are more economical than ginseng roots, stable in terms of supply, and economically advantageous. In addition, it is known that the saponin content is higher in ginseng leaves and ginseng flowers than in ginseng root. To date, studies related to ginseng flowers and buds (flowers that have not yet bloomed due to the development of a significant amount of flower buds just before the ginseng flower blooms and are close to flowering) are analysis of the fragrance components of ginseng flowers (Changsik Woo, 2009. Ajou University). Master's thesis), Skin improvement effect of ginseng extracts by site (Doh Hee-jin, 2013, Master's thesis at Seokyeong University), Effect of oral administration of fermented ginseng flower extract on the production of mouse cytokines IL-6 and TNF-α (Sooji Jeong et al. , 2014. Korean Journal of Food and Nutrition 27: 43-49) and antioxidant activity of ginseng flowers by fermenting microorganisms (Kyunghee Kim et al., 2013. Korean Journal of Food Science and Nutrition 42: 663-669), etc. It is a report of activity.

Patents related to ginseng buds include Korean Patent Registration No. 10-1707522 [A composition for external use for skin containing ginseng flower extract], and No. 10-1784992 [Ginseng flower powder and its manufacturing method, and a cosmetic composition containing the same. ], No. 10-1656710 [Method for preparing ginseng flower complex extract with enhanced active substance, and functional cosmetic composition prepared therefrom], No. 10-1944039 [composition for animal feed using ginseng flower], No. 10 -0017300 [Shampoo and conditioner composition added by stabilizing ground sub-extracts of ginseng], No. 10-1601022 [External composition for skin containing ginseng flower essential oil], No. 10-1438368 [Scent of ginseng flower] Most of the registered patents related to cosmetics, such as [Ginsenoside Rg3 enhancement through fermentation of ginseng flower black yeast and cosmetic composition containing the same as an active ingredient] in No. 10-1663946], No. 10-1944039 No. [Composition for animal feed using ginseng flower], No. 10-0894029 [Pharmaceutical composition for the prevention and treatment of diabetes using ginseng flower], No. 10-1016996 [Ginsenoside] Containing a composition for preventing and treating cardiovascular diseases], No. 10-0004149 discloses a method for producing ginseng bud tea. In addition, Korean Patent Application Laid-Open No. 10-2018-0088226 [composition containing ginseng flower extract with enhanced ginsenoside content as an active ingredient] and 10-2013-0050568 [Ginseng flower with irritation relief and whitening ability] Methods of manufacturing fermented products] and the like are disclosed. However, until now, there is no known ginseng bud extract that exhibits antibacterial activity against various Gram-positive bacteria and Gram-negative bacteria related to allergic food poisoning such as Proteus vulgaris .

KR 10-2018-0088226 A

Heejin Do, 2013, Master's thesis at Seokyeong University, Skin improvement effect of ginseng extracts

The present invention has been devised to solve the problems of the prior art as described above, and an object to be solved in the present invention is to provide a broad-spectrum antibacterial composition containing a ginseng bud extract as an active ingredient.

In order to solve the above problems, the present invention provides an antibacterial composition containing an extract of ginseng bud (Flower bud of Panax ginseng CA Meyer) as an active ingredient.

The ginseng bud extract is preferably an ethanol extract of ginseng bud.

It is preferable that the antibacterial composition has antibacterial activity against Gram-positive bacteria.

The antibacterial composition preferably has antibacterial activity against Proteus vulgaris .

The antimicrobial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, animal feed additives, detergents and cleaners.

Ginseng bud extract as an active ingredient of the broad-spectrum antibacterial composition of the present invention, as demonstrated through the examples of the present specification, antibacterial pharmaceutical composition, health functional food, cosmetic composition, animal feed composition, food, cosmetic or animal feed It has an excellent effect that can be used for additives, various cleaning agents, and various cleaning agents. In addition, the ginseng bud extract of the present invention has excellent thermal stability and does not show any loss of activity even in the acidic condition of pH 2 and plasma, so it can be easily processed into various forms such as liquid, cream, powder, pills, and tablets. It can be very useful in pharmaceutical industry, food industry, livestock industry, health industry and cosmetics industry.

1 shows the ginseng root, ginseng leaf, ginseng flower peak and ginseng seed used in the embodiment of the present invention: 1: ginseng root, 2: ginseng leaf, 3: ginseng flower peak, 4: ginseng seed

Hereinafter, the present invention will be described in detail.

The inventors of the present invention prepared a ginseng bud extract by a certain method in order to test the antibacterial effect on ginseng buds, and recovered it as an antibacterial active ingredient, and the extract has excellent thermal stability and acid stability. By confirming the ginseng bud extract was to be utilized as an antibacterial composition.

In particular, the active extract showed a strong antibacterial activity against Proteus vulgaris among Gram-positive bacteria and Gram-negative bacteria, and it was confirmed that it can be developed as a broad-spectrum antibacterial agent against various bacterial infectious diseases, and the present invention was completed.

Specifically, in order to develop an antibacterial composition using ginseng buds known to have anticancer, anti-inflammatory, hepatoprotective, and antihypertensive effects, the present inventors prepared ethanol extracts for ginseng buds, and various bacteria and fungi thereof the evaluation of the antibacterial activity on, exhibits a strong antibacterial activity against the ginseng and a strong antibacterial activity against the bud extract of gram-positive bacteria, Gram-negative bacteria of the known food poisoning bacteria Proteus vulgaris (Proteus vulgaris), wherein the said extract broad It was confirmed that it can be used as a bacterial agent.

Accordingly, the present invention provides an antibacterial composition containing a ginseng bud extract as an active ingredient.

The ginseng bud extract is preferably extracted with a solvent selected from the group consisting of water, alcohol, and anhydrous or hydrous alcohol having 1 to 4 carbon atoms, and ethanol extraction is most preferred.

The ginseng bud extract may be sequentially or fractionated with an organic solvent of hexene, ethyl acetate and butanol to additionally obtain a hexene fraction, an ethyl acetate fraction, a butanol fraction, and a water residue.

In addition, the antimicrobial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, animal feed additives, detergents and cleaning agents.

Hereinafter, a method for producing a ginseng bud extract of the present invention and an efficacy experiment will be described in more detail.

The inventors of the present invention to achieve the object of the present invention, preparing a ginseng bud extract; Evaluating the antibacterial activity of the extract; And the stability investigation step of the ginseng bud extract was performed.

The "ginseng bud extract" included in the composition of the present invention includes the steps of extracting the buds of 3-4 year old ginseng with an organic solvent and filtering the extract using a filter net of 0.06 mm or less, and concentrating it under reduced pressure. Can be obtained by

The solvent used in the present invention includes water (cold water, hot water), alcohol, anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, alcohol, propanol, butanol, etc.), and a mixed solvent of the lower alcohol and water. Hot water, or 95% ethanol extraction is most preferred.

As a preferred embodiment, the ginseng buds of the present invention may be extracted with hot water or ethanol. In addition, the hot water or ethanol extract may be sequentially or fractionated with an organic solvent of hexene, ethyl acetate and butanol to additionally obtain a hexene fraction, an ethyl acetate fraction, a butanol fraction and a water residue.

The ginseng bud extract of the present invention may be prepared as a powder through a conventional powdering process such as vacuum drying, freeze drying, or spray drying. The ginseng bud extract is not decomposed by various degrading enzymes in plasma, and maintains antimicrobial activity even at 100℃ heat treatment and pH 2 in the stomach.

The ginseng bud extract of the present invention exhibits strong antibacterial activity, and is used in pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, foods, cosmetics, additives for animal feed, and various cleaning agents related to the prevention or treatment of infection of pathogenic bacteria. Can be used as a material.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition is formulated in various forms such as oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and injections of sterile injectable solutions according to the usual method for each purpose of use. It may be used, and may be administered orally, or administered through various routes including intravenous, intraperitoneal, subcutaneous, rectal, and topical administration. Such pharmaceutical compositions may further include a carrier, excipient, or diluent, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil And the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-aggregating agent, a lubricant, a wetting agent, a fragrance, an emulsifying agent, a preservative, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" refers to an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, Sensitivity to drugs, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and this can be easily determined by a person skilled in the art.

The effective amount of the component having antimicrobial activity in the pharmaceutical composition of the present invention may vary depending on the age, sex, and body weight of the patient, and generally 1 to 5,000 mg, preferably 100 to 3,000 mg per body weight is administered daily or every other day. Alternatively, it can be administered in 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, the severity of the disease, sex, weight, age, etc., the dosage amount is not limited by any method. The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration can be expected and can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dura mater or intra-cerebroventricular injection. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the route of administration of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to target cells. In the present invention, the "object" is not particularly limited, but includes, for example, human, monkey, cow, horse, sheep, pig, chicken, turkey, quail, cat, dog, mouse, mouse, rabbit, or guinea pig And, preferably, a mammal, more preferably a human.

As a preferred embodiment, the antimicrobial composition of the present invention can be applied as a health functional food.

The health functional food of the present invention can be used in various ways such as effective foods and beverages that can lower the likelihood of infection from a subject having a high risk of infection by pathogenic microorganisms. Foods containing an active ingredient having excellent antibacterial activity of the present invention include, for example, various foods, beverages, gums, teas, vitamin complexes, health supplement foods, etc., in the form of powders, granules, tablets, capsules or beverages Can be used as In general, the active ingredient of the present invention may be added in an amount of 0.01 to 15% by weight of the total food weight, and the health beverage composition may be added in an amount of 0.02 to 10g, preferably 0.3 to 1g, based on 100 ml. The health functional food of the present invention may contain the compound as an essential component in the indicated ratio as an additional component, as well as food supplementary additives acceptable for food, such as natural carbohydrates and various flavoring agents. Examples of the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, taumatin, rebaudioside A, glycyrrhizin, saccharin, aspartame, and the like may be used. The proportion of the flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes a variety of nutrients, vitamins, minerals, synthetic flavors, and flavoring agents such as natural flavors, colorants and thickeners, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids. It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain pulp for the production of natural fruit juices, fruit juice drinks, and vegetable drinks. These components may be used independently or in combination. The proportion of these additives is generally selected from 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

As a preferred embodiment, the antimicrobial composition of the present invention can be applied as a use of a cosmetic composition.

The cosmetic composition of the present invention may be prepared in the form of ingredients having excellent antibacterial activity of the present invention, for example, essence, nutritional cream, body lotion, conditioner, shampoo, body cleanser, lotion, skin, mask pack, etc. It is not limited to this. Manufacturing in this form may be easily performed according to various manufacturing processes known to those skilled in the art.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an animal feed composition.

The animal is a group of organisms corresponding to plants, mainly ingesting organic matter as nutrients, and refers to a differentiated digestion, embryonic and respiratory organs, specifically echinoderm, crustacean, molluscs, fish, amphibians, reptiles, birds, It may be a mammal, and preferably, echinoderm animals such as sea urchins or sea cucumbers, arthropods including crustaceans such as crabs, shrimp, and shrimp, cephalopods, gastropods or mollusks such as bivalve, red snapper, bream, cod, flounder , Fish such as halibut, birds including poultry such as pheasants or chickens, or mammals such as pigs, cows, and goats.

The composition for animal feed may further include grain, vegetable protein feed, animal protein feed, sugar or dairy product to the antibacterial composition containing the antibacterial component of the present invention. The grain may be specifically crushed or crushed wheat, oats, barley, corn, and rice, and the vegetable protein feed may specifically include rapeseed, soybean and sunflower as main components, and the animal protein feed may be specifically May be blood meal, meat meal, bone meal and fish meal, and the sugar or dairy product may specifically be a dry component composed of various milk powder and whey powder.

The composition for animal feed may further include ingredients such as nutritional supplements, digestion and absorption enhancers, sexual ulcer stimulators or disease prevention agents.

The antimicrobial composition contained in the composition for animal feed of the present invention may vary depending on the purpose of use and conditions of use of the feed, for example, 0.1g to 100g of the antimicrobial composition may be included based on 1 kg of the final produced feed.

In addition, the feed composition may be prepared as a hard-viscous granulated or granular material depending on the degree of pulverization of the ingredients, and the composition may be supplied as a mesh or may be formed into a desired separated shape for further processing and packaging, and storage For ease of storage, it may be subjected to a process of pelletizing, swelling or extrusion, and preferably excess water may be removed by drying.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an additive for food, cosmetics or animal feed.

The additive is added to impart preservability to food, cosmetics, or animal feed to be used, and the antibacterial composition is a natural component and is not harmful to the human body, so it can be safely applied to food, cosmetics or animal feed.

The additive may further include additional additives appropriately selected according to the purpose and use of the additive, and may be used in combination with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The additive may be prepared by powdering, granulating, tableting, or liquefying according to the purpose and use thereof, and a conventional method may be used for packaging for commercialization.

In addition, the amount of the antimicrobial composition among the additives may be appropriately used depending on the purpose of use, application form, application site, and application object, and may include, for example, 0.01 parts by weight to 50 parts by weight based on the total composition. The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts if it can achieve the purpose of application due to the characteristics of the product.

As a preferred embodiment, the antimicrobial composition of the present invention can be applied as a cleaning agent.

The detergent may be preferably a detergent for clothes, a dishwasher, a food detergent, or a home appliance detergent. Since the antibacterial composition is a natural component and is not harmful to the human body, more preferably, it can be used for the purpose of washing various household items including kitchen utensils or food washing or for imparting antibacterial properties.

The detergent may additionally contain selected additives according to its purpose and use, and may be used by mixing with other active ingredients such as commonly used detergents or additives such as pigments, surfactants, and preservatives. The detergent may be prepared by powdering, granulating, tableting, or liquefying depending on the purpose and use thereof, and a conventional method may be used for packaging for commercialization.

In addition, the amount of the antimicrobial composition among the detergents may be appropriately used depending on the purpose of use, application type, application site, and the product to be applied, and may include, for example, 0.01 parts by weight to 50 parts by weight based on the total composition, but the antimicrobial properties The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as it can achieve the purpose of application due to the nature of the product.

As a preferred embodiment, the antimicrobial composition of the present invention can be applied as a cleaning agent.

The cleaning agent may be used as a hand, oral, or feminine cleaning agent in a simple manner in order to prevent infection in the body of pathogenic microorganisms that are often susceptible to infection in daily life. Since the antibacterial composition is a natural component and is not harmful to the human body, it can be safely applied even when used as a cleanser for hands, mouth, and women.

The cleaning agent may further contain additional additives appropriately selected according to the purpose and use of the cleaning agent, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The cleaning agent may be prepared by powdering, granulating, tableting, or liquefying depending on the purpose and use of the cleaning agent, but in the case of a liquid type, convenient use will be possible.

In addition, the amount of the antimicrobial composition among the cleaning agents may be appropriately used depending on the purpose of use, application form, and application site, and may include, for example, 0.01 parts by weight to 50 parts by weight based on the total composition, but the content of the antibacterial composition is It is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts if it can achieve the purpose of application due to the characteristics of the product.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only one preferred specific example of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example 1: Preparation of extracts for each part of ginseng and analysis of their useful components

In 2018, the roots, leaves, and buds of 4-year-old ginseng were collected in Yeongju, Gyeongbuk, and used after hot air drying (60℃) in a conventional method. Ginseng fruit was harvested in July, and the collected seeds were put in a mesh bag, soaked in running water for about a day, then rubbed with hands to completely remove the flesh, and then used in the shade for 1 day (Fig. 1). Each ginseng site was coarsely pulverized and extracted with ethanol (95%), and ethanol (95%) was added to the sample weight, extracted three times at room temperature, collected and filtered, concentrated under reduced pressure to prepare a powder, and ethanol extract Was prepared. On the other hand, the ethanol extract of ginseng seed was divided into an oil part in an upper layer and a sediment part in a lower layer when concentrated under reduced pressure, and these were respectively recovered. In addition, the ginseng seed oil was separately recovered by machine milking.

[Table 1] Recovery rate of extracts and milking oil obtained from each part of ginseng

As shown in Table 1, the recovery rate of the ethanol extract for each site of ginseng was in the order of seeds (8.4%)> leaves (4.3%)> flower peaks (1.3%)> roots (0.3%), ginseng In the case of machine milking of seeds, the recovery rate was about 5%. The machine milking oil of ginseng seeds was a dark brown oil, but the oil produced in the upper layer after the ethanol extract of ginseng seeds was concentrated under reduced pressure showed a bright yellow color. The ginseng bud extract was able to recover about 1.3 g for 100 g of ginseng bud, and the spicy taste peculiar to ginseng flower hardly appeared.

Example 2: Analysis of useful components of extracts and oils obtained from ginseng parts

The samples obtained in Example 1 were measured for total polyphenol, total flavonoid, total sugar and reducing sugar content. For the total polyphenol content, 50 μl of Folin-ciocalteau and 100 μl of Na ₂ CO ₃ saturated solution were added to 400 μl of the extraction sample, and the absorbance was measured at 725 nm after standing at room temperature for 1 hour. Tannic acid was used as a standard reagent. For the total flavonoid content, each sample was extracted with methanol for 18 hours, and 4 ml of 90% diethylene glycol was added to 400 μl of the filtered extraction sample solution, and 40 μl of 1 N NaOH was added again, and the absorbance was measured at 420 nm after 1 hour reaction at 37°C. . Rutin was used as a standard reagent. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method.

[Table 2] Analysis of useful components of extracts and oils by ginseng part

As shown in Table 2, as a result of analysis of useful components of extracts and oils by various parts of ginseng, the total polyphenol content was the highest in ginseng leaf extract, showing the highest content of 22.5mg/g, leaf extract> bud extract> root Extracts ≒ sediment of seed extract> upper oil of seed extract> seed milking oil. The total flavonoid content measurement result also showed the highest 13.4mg/g in ginseng leaf extract, followed by leaf extract> bud extract> root extract> sediment of seed extract> top oil of seed extract ≒ seed milking oil. In the case of total sugar and reducing sugar content, leaf extract> flower bud extract> sediment of seed extract> root extract> upper layer oil of seed extract ≒ seed milking oil. These results show that it is possible to develop functional materials using ginseng leaves and flower buds that are discarded without special use.

Example 3: Evaluation of antibacterial activity of extracts and oils for each ginseng site

The antibacterial activity of the ginseng extract and oil prepared in Example 1 was evaluated, and the results are shown in Table 3. As the strain used in the antibacterial activity evaluation, a Gram-negative bacteria Escherichia coli KCTC 1682, Proteus vulgaris KCTC 2433, Pseudomonas aeruginosa KACC 10186, was used Salmonella typhimurium KCTC 1926, Gram-positive bacteria include Staphylococcus aureus KCTC 1916, Staphylococcus epidermidis ATCC 12228, Listeria monocytogenes KACC 10550, Bacillus subtilis KCTC 1924 were used. Meanwhile, Saccharomyces cerevisiae IF0 0233 and Candida albicans KCTC 1940, the causative agent of candidiasis fungal infection, were used to evaluate antifungal activity.

First, in the case of antibacterial activity evaluation, each bacteria was inoculated into Nutrient broth (Difco Co., USA) and cultured at 37° C. for 24 hours, and then each strain was adjusted to OD ₆₀₀ 0.1 to obtain Nutrient agar (Difco Co., USA). , USA) A sterile petri dish (90mm, Green Cross, Korea) containing the medium was plated at 100μl each, and 5μl of each sample was added to a sterile disc-paper (diameter 6.5mm, Whatsman No. 2), and 37°C for 24 hours. In the case of fungi, in the case of fungi, after incubation at 30° C. for 24 hours in the same manner using Sabouraud dextrose (Difco Co. USA), the size of the growth inhibition ring was measured to evaluate the antibacterial activity. As a control, the antibacterial agent ampicillin and the antifungal agent myconazole (miconazole, Sigma Co., USA) were used at a concentration of 1 μg/disc, respectively, and the size of the growth inhibition ring was the diameter of the part where growth did not appear with the naked eye. It was measured in mm and expressed as a representative result after three or more evaluations.

[Table 3] Antibacterial activity of extracts and oils by ginseng site

As a result, as shown in Table 3, ampicillin, which is used as an antibacterial agent in clinical practice, exhibited broad antimicrobial activity even at 1 μg/disc, and showed relatively weak growth inhibition rings (9 to 15 mm) for Pseudomonas aeruginosa and Salmonella typhimurium . Done. Miconazole, used clinically as an antifungal agent, strongly inhibited the growth of Saccharomyces cerevisiae and Candida albicans even at 1 μg/disc. On the other hand, ginseng bud extract showed excellent growth inhibition against Gram-positive bacteria Listeria monocytogenes , Bacillus subtilis , Staphylococcus epidermidis , Staphylococcus aureus, and Gram-negative bacteria Proteus vulgaris at a concentration of 500 μg/disc.

Example 4: Plasma, acid and thermal stability evaluation of ginseng bud extract

Plasma stability, thermal stability, and acid stability against antimicrobial activity were confirmed for the ethanol extract of ginseng bud obtained in Example 1 above. As a result, the active extract exhibited high stability without significant loss of antimicrobial activity even after 1 hour heat treatment at 100°C, 1 hour treatment at pH 2 (0.01M HCl), and 1 hour treatment in plasma. Therefore, the ethanol extract of ginseng buds is expected to maintain excellent antibacterial activity even under various food processing processes.

Claims (5)

Ginseng bud (Flower bud of Panax ginseng CA Meyer) antibacterial composition containing the extract as an active ingredient. The antibacterial composition of claim 1, wherein the ginseng bud extract is an ethanol extract of ginseng bud. The antibacterial composition of claim 2, wherein the antibacterial composition has antibacterial activity against Gram-positive bacteria. The antimicrobial composition of claim 2, wherein the antimicrobial composition has antibacterial activity against Proteus vulgaris . The method according to any one of claims 1 to 4, wherein the antibacterial composition is selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, animal feed additives, detergents and cleaners. Antibacterial composition, characterized in that the.

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