KR20180088226A - Composition comprising ginseng flower extracts with enhanced ginsenoside content - Google Patents

Abstract

In this specification, a composition containing an effective ingredient of ginseng flower extract with enhanced ginsenoside content, a composition having a skin barrier function-enhancing use and a process for producing the composition are disclosed. The composition increases the expression of the genes hBD2, hBD3, and psoriasin of the antimicrobial peptide which acts as an antibacterial barrier on the skin surface, thereby enhancing the skin barrier function.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition containing ginseng flower extract having enhanced ginsenoside content as an effective ingredient. BACKGROUND ART < RTI ID = 0.0 >

In this specification, a composition containing an effective ingredient of a ginseng flower extract having enhanced ginsenoside content and having an application for enhancing skin barrier function and a method for producing the composition are disclosed.

Ginseng (Panax ginseng C.A. Meyer) is a herb that belongs to the genus Ogawa and Ginseng. It has been used in Korea, China and Japan for over 2,000 years and has been used empirically to prevent disease and prolong the life span. The efficacy and effect of ginseng which has been known so far can be determined by the action of the central nervous system, the anticarcinogenic action, the anticancer activity, the immune function regulating action, the anti-diabetic action, the hyperfunction of liver function, the improvement of cardiovascular disorder, Improvement of disorder, effect on osteoporosis, anti-stress and anti-fatigue action, antioxidant activity and anti-aging effect.

Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. Especially, ginsenoside (root), ginseng leaf and ginseng berries have not only ginsenoside content but also composition .

Recently, consumers are interested in natural cosmetics. At the same time, many cosmetics using herbal materials have been launched, and ginseng has been studied as a plant material having important skin effect of cosmetics. However, most of them are ginseng root extract or ginseng root ginsenoside Side effects and ginseng polysaccharides are used only. The effect of various components of ginseng such as ginseng fruit is insufficient. In addition, the value of the ginseng flower has not been known for a long time. Recently, the ginseng flower has a distinctive fresh fragrance and its value as a fragrant plant has been noted, and a fancy composition recreating the fragrance of an excellent ginseng flower has begun to be developed. However, the effect of skin barrier function of ginseng flower is still unknown, and research on it is insufficient.

On the other hand, the conventional method of obtaining an extract from ginseng has a problem in that it is inadequate to lower the cytotoxicity while maintaining the pharmacological component of ginseng.

KR10-2013-0039470 A1

In one aspect, an object of the present invention is to provide a novel use of ginseng flower extract having enhanced ginsenoside Re content.

In another aspect, an object of the present invention is to provide a composition containing an extract of ginseng flower as an active ingredient and having enhanced skin barrier function.

In another aspect, an object of the present invention is to provide a method for producing the composition.

In one aspect, the present invention provides a composition for enhancing skin barrier function comprising, as an active ingredient, a ginseng flower extract containing ginsenoside Re in an amount of 2.30 parts by weight or more when the dried material of ginseng flower as an extraction raw material is 100 parts by weight.

In another aspect, the present invention provides a method for producing the composition, which comprises adding water, an organic solvent or a mixed solvent of water and an organic solvent to a ginseng flower, and repeating the step of pressurization and depressurization to extract .

In one aspect, a composition comprising, as an active ingredient, a ginseng flower extract containing ginsenoside Re in an amount of 2.30 parts by weight or more when the dried material of the ginseng flower of the present invention is 100 parts by weight has an antimicrobial barrier It increases the expression of the genes hBD2, hBD3 and psoriasin of the antimicrobial peptide, thus enhancing the skin barrier function.

FIG. 1 is a graph showing the effect of the ginseng flower extract (Comparative Example 3) extracted with the general extraction process (atmospheric pressure condition) (Comparative Example 3) and the ginseng flower extract (at the pressure of 80 MPa under pressure extraction) according to one embodiment of the present invention And the component of the ginsenoside of Example 3).
FIG. 2 is a graph showing the results of a comparison between the ginseng flower extract (Comparative Example 4) extracted with hot water extraction (Comparative Example 4), the ginseng flower extract extracted with the general extraction process (atmospheric pressure condition) (HBD2, hBD3, and psoriasin) of the ginseng flower extract (Example 3) extracted with the extraction process (pressure of 80 MPa at the time of pressurization extraction) according to the method of the present invention.

Hereinafter, the present invention will be described in detail.

In one aspect, the present invention provides a composition for enhancing skin barrier function comprising, as an active ingredient, a ginseng flower extract containing ginsenoside Re in an amount of 2.30 parts by weight or more when the dried material of ginseng flower as an extraction raw material is 100 parts by weight.

The ginseng flower of the present invention ( Panax ginseng CA Meyer) belongs to the above-ground part of ginseng, and its ginsenoside content is more than roots and its kind is similar to roots. The undifferentiated flowers are removed to promote root growth of ginseng.

The ginsenoside of the present invention is a kind of saponin which is a kind of glycoside, and is derived from root, stem, leaf, skin, seed and the like of a plant. There are various kinds of ginsenosides, for example, ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1 and the like, and they exist depending on kinds of plants, cultivation conditions, processing conditions, The kind and content of ginsenoside are different. Rb1, Rb2, Rc, Rd, Re and Rg1 can be divided into PPT (Re, Rg1) and PPD (Rb1, Rb2, Rc and Rd) It is water-soluble as a polar substance which has not undergone denaturation by removing sugars through oxidation.

The ginseng flower extract of the present invention contains 2.30 parts by weight or more, 2.32 parts by weight or more, 2.34 parts by weight or more, 2.36 parts by weight or more, 2.38 parts by weight or more, 2.40 parts by weight or more, 2.40 parts by weight or more, 2.42 weight parts or more, 2.44 weight parts or more, 2.46 weight parts or more, 2.48 weight parts or more, 2.48 weight parts or more, 2.49 weight parts or more, 2.50 weight parts or more, 2.52 weight parts or more, 2.54 weight parts or more, At least 2.60 parts by weight, at least 2.62 parts by weight, at least 2.63 parts by weight, at least 2.64 parts by weight, at least 2.64 parts by weight, at least 2.66 parts by weight, at least 2.68 parts by weight, at least 2.70 parts by weight, at least 2.72 parts by weight, at least 2.74 parts by weight, At least 2.78 parts by weight, at least 2.78 parts by weight, at least 2.8 parts by weight, at least 2.82 parts by weight, at least 2.84 parts by weight, at least 2.86 parts by weight, at least 2.88 parts by weight, at least 2.90 parts by weight, at least 2.92 parts by weight, at least 2.94 parts by weight, Not less than 2.98 parts by weight, or not less than 3.0 parts by weight The can be included.

The ginseng flower extract of the present invention contains 0.25 parts by weight or more, 0.26 parts by weight or more, 0.27 parts by weight or more, 0.28 parts by weight or more, 0.29 parts by weight or more, or 0.30 parts by weight or more of ginsenosides Rb1, and may comprise at least 0.20 parts by weight, at least 0.21 parts by weight, at least 0.22 parts by weight, at least 0.23 parts by weight, at least 0.24 parts by weight, or at least 0.25 parts by weight of ginsenoside Rb2, or at least 0.20 parts by weight 0.22 weight parts or more, 0.24 weight parts or more, 0.26 weight parts or more, 0.28 weight parts or more, 0.30 weight parts or more, 0.31 weight parts or more, 0.32 weight parts or more, 0.33 weight parts or more, or 0.34 weight parts or more of ginsenoside Rc can do. The ginseng flower extract of the present invention may contain at least 0.60 part by weight, at least 0.62 part by weight, at least 0.64 part by weight, at least 0.66 part by weight, at least 0.68 part by weight, at least 0.70 part by weight At least 0.72 part by weight, at least 0.74 part by weight, at least 0.76 part by weight, at least 0.78 part by weight, at least 0.80 part by weight, at least 0.81 part by weight, at least 0.82 part by weight, at least 0.83 part by weight or at least 0.84 part by weight of ginsenoside Rd Or 0.25 weight parts or more, 0.26 weight parts or more, 0.27 weight parts or more, 0.28 weight parts or more, 0.29 weight parts or more, 0.30 weight parts or more, or 0.31 weight parts or more of ginsenoside Rg1. According to one embodiment of the present invention, the ginseng flower extract prepared in the pressure and reduced pressure extraction process under the pressure condition at the time of extracting the pressure of 80 MPa, the pressure at 80 MPa, To 3) showed higher yields than the ginseng flower extracts prepared in the general extraction process (Comparative Examples 1 to 3), and the absolute amount (g) of ginsenoside Re was increased on the basis of 100 g of ginseng flower powder. (Test Examples 1 to 3 and Fig. 1).

The skin barrier (Stratum corneum) of the present invention is composed of dead keratinocyte (intercellular lipid) and intercellular lipid. It protects the skin from external stimuli and protects the skin from evaporation of moisture. As a function of skin health.

The antimicrobial peptide of the present invention is an antimicrobial peptide related to an antibacterial barrier on the skin surface. As the expression amount of the antimicrobial peptide gene increases, the skin barrier function can be further strengthened. The antimicrobial peptide gene of the present invention may be one or more genes selected from the group consisting of human beta defensin 2, human beta defensin 3 and human beta defensin 3, hBD2, human beta defensin 3 and horseradish peroxidase have. The hBD2 and hBD3 are a type of defensin family in mammals and Beta-defensin has antimicrobial peptide function, so that it can be used in a very large number of Gram-negative or gram- -positive) shows resistance to bacteria and fungi. The frostassin, also known as S100A7, is a class of the S100 protein family, secreted and deposited in skin epithelial cells, contributing to the formation of an antibacterial barrier. According to one embodiment of the present invention, the ginseng flower extract (Example 3) prepared under the pressure condition for 24 hours, water temperature of 30 DEG C, pressure at 80 MPa pressure extraction, (HBD2, hBD3, and frostin) gene expression was found to be higher than that of the control (no treatment group), the hot water extract (Comparative Example 4), and the extract prepared by the ordinary extraction process under atmospheric pressure (Comparative Example 3) It was confirmed that the ginseng flower extract of the present invention containing the bar and the ginsenoside Re above a specific content has an effect of enhancing the skin barrier function by increasing the expression of the antimicrobial peptide (Test Example 4 and FIG. 2).

The ginseng flower extract of the present invention can be used without limitation as long as it can produce ginseng flower extract containing 2.30 parts by weight or more of ginsenoside Re when the dried ginseng flower is 100 parts by weight. Process can be used. The pressurized and reduced pressure extraction process may be a process of performing a pressurization process or a depressurization process that changes the pressure during the extraction process. Specifically, the ginseng flower extract of the present invention can be produced by a pressure circulation extraction process or the like which alternately performs a pressurization process or a depressurization process, and they can be carried out continuously. Specifically, the pressure circulation and extraction process may be performed by adding water, an organic solvent or a mixed solvent thereof to the ginseng flower, and repeating the pressurization and decompression at regular intervals. More specifically, the pressurized and reduced pressure extraction process may be repeated for at least 18 hours, at least 19 hours, at least 20 hours, at least 21 hours, at least 22 hours, at least 23 hours, or at least 24 hours, Hour, 28 hours or less, 27 hours or less, 26 hours or less, 25 hours or less, or 24 hours or less. The extraction temperature may be 25 ° C or higher, 26 ° C or higher, 27 ° C or higher, 28 ° C or higher, 29 ° C or higher or 30 ° C or higher, and 35 ° C or lower, 34 ° C or lower, Deg.] C, 31 [deg.] C or less, or 30 [deg.] C or less. The pressure during the pressure extraction process may be at least 60 MPa, at least 65 MPa, at least 70 MPa, at least 75 MPa, or at least 80 MPa and may be at most 100 MPa, at least 95 MPa, at least 90 MPa, at most 85 MPa, or at most 80 MPa, Lt; / RTI >

According to one embodiment of the present invention, the optimum conditions for the pressure and decompression extraction processes for producing the ginseng flower extract having the best yield and the absolute amount of ginsenoside Re are 24 hours of treatment time, the temperature of water at 30 ° C and the pressure of 80 MPa It was confirmed that the pressure condition at the time of extraction (Test Example 2).

The composition of the present invention may contain 0.0001 wt% to 99 wt%, specifically 0.1 wt% to 99 wt%, more specifically 1 wt% to 50 wt% of ginseng flower extract based on the total weight of the composition.

In this aspect, the composition may be a pharmaceutical composition, a cosmetic composition, or a food composition.

The pharmaceutical composition may be formulated into an oral or parenteral dosage form in the form of a solid, semi-solid or liquid form by adding an inorganic or organic carrier, which is compatible with the composition, as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, soft capsules, powders, fine granules, powders, emulsions, syrups and pellets. Examples of the parenteral administration agent include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention is excellent in the effect of strengthening the skin barrier, and thus can be useful for the treatment and prevention of skin diseases caused by damage to the skin barrier. Skin diseases caused by skin barrier damage include, but are not limited to, atopic dermatitis, xeroderma, psoriasis, ichthyosis, acne and the like.

The pharmaceutical composition may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

In addition, the dosage of the active ingredient will depend on the age, sex, body weight of the subject to be treated, the particular disease or condition to be treated, the severity of the disease or condition, the route of administration and the judgment of the prescriber. Dosage determinations based on these factors are within the level of those skilled in the art. Typical dosages are from 0.001 mg / kg / day to 2000 mg / kg / day, specifically from 0.5 mg / kg / day to 1500 mg / kg / day.

The cosmetic composition may be provided in all formulations suitable for topical application. For example, it may be provided as a liquid, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an oil phase in water, a suspension, a solid, a gel, a powder, a paste, a foam or an aerosol composition. Compositions of such formulations may be prepared according to conventional methods in the art.

The cosmetic composition may contain, in addition to the above-mentioned materials, other ingredients that do not impair the main effect, specifically, those that can give synergy to the main effect. The cosmetic composition according to the present invention may include a material selected from the group consisting of vitamins, high molecular peptides, molecular polysaccharides and sphingolipids. The cosmetic composition according to the present invention may also contain a moisturizing agent, an emollient agent, a surfactant, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, . The blending amount of the above components can be easily selected by those skilled in the art within a range not to impair the object and effect of the present invention. The blending amount thereof can be 0.001 to 5% by weight, specifically 0.01 to 3% have.

In the above aspect, the pharmaceutical composition or cosmetic composition may be a composition for external application to the skin. For example, it may be cream or ointment.

The food composition may be a health food composition, and may be a fermented food composition requiring fermentation, such as tea, dairy, kimchi, and brewed foods. The formulation of the food composition is not particularly limited, but may be formulated into tablets, pills, soft and hard capsules, granules, drinks, caramels, diet bars, tea bags and the like. The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients. The dosage of the active ingredient is within the level of those skilled in the art and may vary depending on various factors such as the age, health condition, complications, etc. of the subject to be administered.

In another aspect, the present invention provides a method for producing a composition comprising adding water or an alcohol as a primary solvent to a ginseng flower and then subjecting the ginseng flower to a primary extraction by repeating the pressure and vacuum extraction steps . The method may further comprise fractionating the extracted primary extract with a chlorine-containing organic solvent, removing the chlorine-containing organic solvent layer, and then extracting the water layer with alcohol again, wherein the primary solvent The alcohol may be dissolved in water before the fractionation with a chlorine-containing organic solvent.

The alcohol may be a lower alcohol having 1 to 5 carbon atoms, including methanol, ethanol, butanol, propanol and isopropanol, and may be an organic solvent such as a polar solvent such as ethyl acetate and a nonpolar solvent such as hexane or methylene chloride, or a mixture thereof It can be used every day. Specifically, the alcohol may be 10 to 90% (v / v) ethanol, more specifically 80% (v / v) ethanol. The primary solvent of the present invention may be water or alcohol, and the secondary solvent may be alcohol. Specifically, the primary solvent may be water, and the secondary solvent may be ethanol.

The chlorine-containing organic solvent may be at least one selected from the group consisting of methylene chloride, chloroform, methyl chloride and carbon tetrachloride, and may be specifically methylene chloride as an organic solvent containing chlorine (chloride) .

The primary solvent, the secondary solvent and the chlorine-containing organic solvent may be the same or different from each other.

When the pressurization and decompression extraction processes are performed as described above, the thermal denaturation of the ginseng flower can be minimized and the denaturing of the ginsenoside can be minimized. Therefore, the extraction yield of the ginseng flower extract can be improved by 10% or more compared to the case of extracting the ginseng flower without using the conventional simple extraction device.

Further, thereafter, the chlorophyll is removed using a chlorine-containing organic solvent such as methylene chloride, and the water layer is secondarily extracted with an organic solvent such as ethanol, thereby maximizing the content of ginsenoside.

Hereinafter, the present invention will be described with reference to the following examples and test examples. The following examples and test examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, but the scope and scope of the present invention are not limited thereto.

[Comparative Example] Production of ginseng flower extract by general extraction process

[Comparative Examples 1 and 2] Preparation of ginseng flower extract powder by a general extraction process

Ginseng flowers obtained from Sangju and Andong, Korea were washed with purified water, dried by hot air or natural drying, and then pulverized to obtain a germinated flower powder. Thereafter, 10 L of water was added to 0.5 kg of the ginseng flower powder, and the mixture was extracted at 30 캜. The extract was filtered and concentrated to a solid content of 50%. Methylene chloride (MC) fractionation was performed to remove chlorophyll from the concentrate. The water layer was then taken, ethanol was added until it became 80% ethanol, and after dipping for 12 hours to remove sugar, the solution was filtered and the precipitate discarded. Then, the filtrate was concentrated until ethanol was removed to prepare 125 g of ginseng flower extract powder (Comparative Examples 1 and 2).

[Comparative Example 3] Analysis of ginsenoside and production of ginseng flower extract according to a general extraction process for confirming the expression of an antibacterial peptide

125 g of ginseng flower extract (Comparative Example 3) was prepared in the same manner as in the above Comparative Examples 1 and 2 under the conditions of 24 hours, water temperature of 30 캜 and atmospheric pressure.

[Comparative Example 4] Production of ginseng flower extract by hot water extraction for confirming expression of an antibacterial peptide

10 L of water was added to 0.5 kg of the ginseng flower (green seedling) powder, and the mixture was extracted at 95 캜. The extract was filtered and concentrated to a solid content of 50%. Then, 125 g of ginseng flower extract (Comparative Example 4) was prepared by performing methylene chloride fractionation, ethanol addition, filtration and concentration in the same manner as in the preparation methods of Comparative Examples 1 and 2 above.

EXAMPLES Preparation of ginseng flower extract by pressurization and decompression extraction process

[Examples 1 and 2] Preparation of ginseng flower extract powder by pressurization and reduced pressure extraction process

Ginseng flowers obtained from Sangju and Andong, Korea were washed with purified water, dried by hot air or natural drying, and then pulverized to obtain a germinated flower powder. Thereafter, 10 L of water was added to 0.5 kg of the ginseng flower powder, and the mixture was extracted for 24 hours with a pressure circulation type extraction device repeatedly performed for 15 minutes each under pressure and reduced pressure. At this time, the pressure during the pressure extraction was 80 MPa, the pressure during the decompression extraction was atmospheric pressure, and the extraction temperature was set at 30 ° C. The ginseng flower extract obtained by the above process was filtered and concentrated to a solid content of 50%. Methylene chloride (MC) fractionation was performed to remove chlorophyll from the concentrate. The water layer was then taken, ethanol was added until it became 80% ethanol, and after dipping for 12 hours to remove sugar, the solution was filtered and the precipitate discarded. Then, the filtrate was concentrated until the ethanol was removed to prepare 125 g of ginseng flower extract powder (Examples 1 and 2).

[Example 3] Analysis of ginsenoside and production of ginseng flower extract by pressurization and depressurization step for confirmation of expression of antimicrobial peptide

In order to analyze the ginsenosides of the extracts obtained by the pressurization and decompression extraction processes, ginseng flowers (female seedling) were cultured for 24 hours, at a temperature of 30 ° C, at a pressure of 80 MPa under pressure, 125 g of ginseng flower extract (Example 3) according to the pressure and reduced pressure extraction steps were prepared in the same manner as in the production methods of Examples 1 and 2.

[Test Example 1] Comparison of the yield according to the extraction process and the absolute amount of ginsenoside Re

The HLPC analysis of the obtained ginseng flower extract powder (Comparative Examples 1 and 2 and Examples 1 and 2) was carried out under the following conditions.

Ginsenoside Re HPLC analysis conditions

- Column: CAPCELL PAK C18 (SHISEIDO)

- Detector (measuring wavelength): Ultraviolet absorptivity detector (203 nm)

- Injection volume, flow rate: 20 uL, 1.0 mL / min

- Mobile phase: Gradient conditions for HPLC (A: D.I WATER, B: 50% BeCN)

time Pump A Pump B 0 50 50 15 35 65 16 0 100 20 0 100 21 50 50 35 50 50 35 Stop

As a result, the yields of the ginseng flower extract powder (Comparative Examples 1 and 2 and Examples 1 and 2) and the absolute amount (g) of ginsenoside Re were as shown in Table 2 below. Absolute amount of ginsenoside Re was 100 g .

Comparison of yield of ginseng flower extract and absolute amount of ginsenoside Re according to extraction conditions Changzhou ginseng
(Comparative Example 1) Andong ginseng
(Comparative Example 2) Pressurization and Decompression
Changzhou ginseng
(Example 1) Pressurization and Decompression
Andong ginseng
(Example 2) yield(%) 18 21 29 31 Re absolute amount (g) 1.83 2.25 2.49 2.98

* Ginsenoside Re absolute amount is based on 100 g of ginseng flower powder.

As a result, as shown in Table 2, the ginseng flower extracts (Examples 1 and 2) extracted according to the pressurization and decompression extraction processes were the same as the ginseng flower extracts (Comparative Examples 1 and 2 ) And 11% and 10%, respectively, and the ginsenoside Re was found to contain 0.66 g and 0.73 g of ginsenoside Re based on 100 g of the ginseng flower powder.

[Test Example 2] Confirmation of optimal conditions of pressurization and decompression extraction process

It was confirmed in Test Example 1 that the yield and the absolute amount of ginsenoside Re in the pressurization and decompression extraction process were superior. In order to confirm the conditions of the optimum pressurization and decompression extraction process, the processing time, temperature and pressure conditions were sequentially changed And the absolute amount of ginsenoside Re (g, based on 100 g of ginseng flower powder) were compared.

Identify optimal time conditions

Under the conditions of the water temperature of 30 DEG C, the pressure at the time of 80 MPa pressure extraction and the pressure at the time of extraction under reduced pressure of the atmospheric pressure, 1 < RTI ID = 0.0 > Hours, 6 hours, 12 hours, and 24 hours, and HPLC analysis was performed under the same analytical conditions as in Test Example 1. The results are shown in Table 3.

Comparison of yield of ginseng flower extract and absolute amount of ginsenoside Re according to time time yield(%) Re absolute amount (g, based on 100 g of ginseng flower powder) 1 hours 33.11 2.48 6 hours 27.51 2.15 12 hours 33.08 2.48 24 hours 33.67 2.76

Identifying Optimum Temperature Conditions

The water temperature was measured under the pressure conditions at the time of pressure extraction at 80 MPa and the pressure at the time of extraction under reduced pressure of atmospheric pressure in the same manner as in the methods of preparing the ginseng flower extract powder of Examples 1 and 2, , 30 ° C. and 40 ° C., and the HLPC analysis was carried out under the same analytical conditions as in Test Example 1, and the results are shown in Table 4.

Comparison of the yield of ginseng flower extract and the absolute amount of ginsenoside Re according to the water temperature Temperature yield(%) Re absolute amount (g, based on 100 g of ginseng flower powder) 20 ℃ 29.10 2.15 30 ℃ 33.67 2.76 40 ℃ 35.98 2.59

Identify optimal pressure conditions

The pressure at the time of pressure extraction was measured in the same manner as in the methods for preparing the ginseng flower extract powder of Examples 1 and 2 under the pressure and decompression extraction processes under the conditions of 24 hours, 30 ° C water temperature, The ginseng flower extract powder was prepared by varying the atmospheric pressure (the same as in the general extraction process in preparation of the ginseng flower extract of Comparative Examples 1 and 2), 10 MPa, 20 MPa, 40 MPa and 80 MPa, HPLC analysis was performed, and the results are shown in Table 5.

Comparison of the yield of ginseng flower extract and the absolute amount of ginsenoside Re according to the pressure during pressure extraction pressure yield(%) Re absolute amount (g, based on 100 g of ginseng flower powder) Atmospheric pressure (control group) 21.58 2.25 10 MPa 31.76 2.26 20 MPa 33.80 2.60 40 MPa 30.49 2.16 80 MPa 33.67 2.76

As a result, as shown in Tables 3 to 5, it was confirmed that the best yield and the absolute amount of ginsenoside Re were observed under the pressure condition at the time of 24 hours, 30 ° C water temperature and 80 MPa pressure extraction.

[Test Example 3] Analysis of ginsenoside

The optimum conditions of the pressurization and decompression extraction processes in Test Example 2 were confirmed. To examine the ginsenosides of the extract according to the pressurization and decompression extraction processes, the ginseng flower extract prepared in the general extraction process (Comparative Example 3) And the ginsenoside content of the ginseng flower extract (Example 3) prepared by the reduced pressure extraction process were analyzed.

The ginseng flower extracts of Comparative Examples 3 and 3 were subjected to HPLC analysis under the same analytical conditions as in Test Example 1, and the results are shown in FIG. From these results, the absolute amount (g, based on 100 g of ginseng flower powder) of each component of ginsenoside contained in the ginseng flower extract is shown in Table 6 below.

Comparative Analysis of Absolute Amounts of Ginsenosides by General Extraction Process and Pressure and Decompression Extraction Process (Unit: g) Rb1 Rb2 Rc Rd Re Rg1 Sum General extraction
(Comparative Example 3) 0.22 0.17 0.16 0.58 1.72 0.22 3.07 Pressurization and Decompression Extraction
(Example 3) 0.30 0.26 0.34 0.84 2.63 0.31 4.68

As a result, as shown in Table 6, the ginseng flower extract (Example 3) according to the pressurization and decompression extraction process contained about 30 to 112 ginsenoside in the ginseng flower extract (Comparative Example 3) % More than the others. It was also confirmed that the total weight of ginsenosides contained in the ginseng flower extract was significantly higher than that of Comparative Example 3.

[Test Example 4] Expression of Antibacterial Peptide Gene

In order to confirm the effect of the ginseng flower extract prepared under the optimum conditions of Test Example 2 on the expression of the antimicrobial peptide gene, the following experiment was conducted. The antimicrobial peptide genes identified in the experiment are hBD2 (human beta defensin 2), hBD3 (human beta defensin 3) and psoriasin.

After keratinocytes were grown using KGM-gold (Lonza, # 00192151) medium, 25,000 keratinocytes were added to a 6-well plate and cultured for 24 hours. (Comparative Example 4), extracts obtained by a general extraction process under atmospheric pressure (Comparative Example 3), pressurized and decompressed extracts with a pressure of 80 MPa (Example 3) were diluted to 40 ppm in the medium And then treated with keratinocytes. At this time, nothing was treated in the control group (untreated group). After reacting for 24 hours, 1 mL of trisole was added to the cells to extract RNA, and homogenized at room temperature for 5 minutes. The mixture was transferred to a 1.5-mL tube, and 0.1 mL of chloroform was added thereto. The mixture was shaken for 15 seconds by hand, allowed to react at room temperature for 15 minutes, and then centrifuged at 13,000 rpm for 15 minutes at 4 ° C. 0.4 mL of the transparent supernatant was transferred to a new tube, 0.5 mL of isopropyl alcohol was added, and the mixture was shaken by hand for 15 seconds. After reacting at room temperature for 8 minutes, the mixture was centrifuged at 13,000 rpm for 8 minutes at 4 ° C. The supernatant was discarded, and 0.5 mL of 75% alcohol was added thereto, followed by centrifugation at 4 ° C and 10,000 rpm for 5 minutes. Only the pellet was left, the supernatant was discarded, and 22 μL of deionized water was added to dissolve the pure RNA. The amount of RNA was quantified by measuring the absorbance at 260 nm using a spectrophotometer, and then, to synthesize cDNA, 3.5 μL of RNA, 3.5 μL of 10 × RT buffer (Invitrogen, # 18080-051), 2 μL of 50 μM oligo dT, ₇ μL of 25 mM MgCl ₂ , ₃ μL of 10 mM dNTP, 1 μL of RNAaseOut ^{™ and} 1 μL of Superscript III were mixed. Then, the remaining amount was added to deionized water so as to be 35 μL. RT reaction was carried out at 50 ° C for 60 minutes using a PCR instrument. To 35 μL of the prepared cDNA mixture, 165 μL of deionized water was added, and the mixture was diluted with a total of 200 μL. To proceed with real-time qPCR, mix 1 μL of 5 μL of cDNA mixture, 4 μL of deionized water, 10 μL of Taqman 2X Universal PCR Master Mix (ABI, # 4304437), Taqman primer (control gene; RPLP0, experimental gene; hBD2, hBD3, psoriasin) A total of 20 μL of the solution was added to each well of a 96-well PCR tube, and the solution was loaded into a Real-time PCR instrument (ABI, Model 7500fast). The reaction setup is as follows. 1. Holding phase: 95 ° C (10 min); 2. Cycling step: 95 ° C (15 sec) and 60 ° C (60 sec) The Taqman primer used in this study was a commercial primer composed of reporter-acting fluorescent molecule FAM, NFQ-MGB, and the expression levels of hBD2, hBD3 and psoriasin genes were compared in Fig.

As a result, as shown in FIG. 2, the extracts of the ginseng flower 80 MPa pressurized and decompressed extracts (Example 3) in the untreated group (control group), hot water extract (Comparative Example 4) 3), the expression of the antimicrobial peptide genes hBD2, hBD3 and the frogasin gene was increased. Therefore, it was found that the ginseng flower extract containing a specific content of ginsenoside, under pressure and reduced pressure under optimum conditions, has an effect of enhancing the skin barrier function by increasing the expression of the antimicrobial peptide.

Formulation examples of compositions according to one embodiment of the present invention are described below, but may be applied to various other formulations, which are not intended to be limiting but merely illustrative of the present invention

[Formulation Example 1] Nutritional lotion

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 7 below.

Raw material name Content (% by weight) glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 The ginseng flower extract of Example 3 1.0 Wax 4.0 Polysorbate 60 1.5 Caprylic / capriciculiglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 2] Nutrition lotion

Nutrition lotions can be prepared in a conventional manner according to the composition shown in Table 8 below.

Raw material name Content (% by weight) Purified water Balance glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 5.0 Beta Glucan 7.0 Carbomer 0.1 The ginseng flower extract of Example 3 1.0 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.5 antiseptic Suitable amount incense Suitable amount Pigment Suitable amount Triethanolamine 0.1 system 100

[Formulation Example 3] Nourishing cream

Nutrition creams can be prepared in a conventional manner according to the composition shown in Table 9 below.

Raw material name Content (% by weight) glycerin 3.5 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 The ginseng flower extract of Example 3 1.0 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 4] Hair pack

The pack can be manufactured in a conventional manner according to the composition shown in Table 10 below.

Raw material name Content (% by weight) glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 The ginseng flower extract of Example 3 1.0 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative Suitable amount Preservative, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 5] Ointment

Ointment can be prepared by a conventional method according to the composition shown in Table 11 below.

Raw material name Content (% by weight) The ginseng flower extract of Example 3 2.0 Beta-1,3-glucan 10.0 Wax 10.0 Polysorbate 5.0 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.5 vaseline 5.0 Liquid paraffin 10.0 Squalane 5.0 Sheer butter 3.0 Caprylic / capric triglyceride 5.0 glycerin 10.0 Propylene glycol 10.2 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance system 100

[Formulation Example 6] Preparation of drug (patch) for topical administration

Pharmaceutical preparations (patches) for topical administration were prepared in a conventional manner according to the compositions shown in Table 12 below.

Raw material name Content (% by weight) The ginseng flower extract of Example 3 2.0 Beta-1,3-glucan 3.0 Diethylamine 0.7 Sodium sulphate 0.1 Polyoxyethylene lauryl ether (E.O. = 9) 1.0 Polyhydroxyethylene cetyl stearyl ether (Cetomacrogol 1000) 1.0 Viscous paraffin oil 2.5 Caprylic acid ester / capric acid ester (Cetiol LC) 2.5 Polyethylene glycol 400 3.0 Polyacrylic acid (Carbopol 934P) 1.0 Purified water Balance system 100

[Formulation Example 7] Preparation of powders

2 g of the ginseng flower extract of Example 3

Lactose 1g

The above components were mixed and filled in airtight bags to prepare powders.

[Formulation Example 8] Preparation of tablets

The ginseng flower extract of Example 3 100 mg

Corn starch 100 mg

Lactose 100mg

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

[Formulation Example 9] Preparation of capsules

The ginseng flower extract of Example 3      100 mg

Corn starch 100 mg

100mg of milk

Magnesium stearate 2 mg

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

[Formulation Example 10] Production of pills

1 g of the ginseng flower extract of Example 3

Lactose 1.5g

Glycerin 1 g

Xylitol 0.5 g

After the above components were mixed, they were prepared to be 4 g per one ring by a conventional method.

[Formulation Example 11] Preparation of granules

150 g of the ginseng flower extract of Example 3

Soybean extract 50mg

200 mg of glucose

Starch 600mg

After mixing the above components, 100 mg of 30% ethanol was added and the mixture was dried at 60 ° C to form granules, which were then filled in a capsule.

[Formulation Example 12]

After mixing 50 mg of the ginseng flower extract of Example 3, 10 g of glucose, 0.6 g of citric acid and 25 g of liquid oligosaccharide, 300 ml of purified water is added, and 200 ml of each is injected into each bottle. After filling the bottle, the beverage can be sterilized at 130 for 4 to 5 seconds.

[Formulation Example 13] Caramel formulation

Mixed with 50 mg of the ginseng flower extract of Example 3, 1.8 g of corn syrup, 0.5 g of skim milk, 0.5 g of soybean lecithin, 0.6 g of butter, 0.4 g of vegetable cured oil, 1.4 g of sugar, 0.58 g of margarine, So that caramel molding can be performed.

Claims (17)

A composition for enhancing skin barrier function comprising, as an active ingredient, an extract of ginseng flower comprising 2.30 parts by weight or more of ginsenoside Re when 100 parts by weight of dried ginseng flower material is extracted. The method according to claim 1,
The ginseng flower extract comprises 0.25 weight parts or more of ginsenoside Rb1, 0.20 weight parts or more of ginsenoside Rb2, 0.20 weight parts or more of ginsenoside Rc, 0.60 weight parts or more of ginsenoside Side Rd and at least 0.25 part by weight of ginsenoside Rg1. The method according to claim 1,
Wherein the composition increases the expression of an antimicrobial peptide gene. The method of claim 3,
Wherein the antimicrobial peptide gene is at least one selected from the group consisting of human beta defensin 2, hBD3, human beta defensin 3 and psoriasin. A composition for enhancing skin barrier function. The method according to claim 1,
Wherein the composition comprises 0.0001 to 99% by weight of the ginseng flower extract based on the total weight of the composition. The method according to claim 1,
Wherein the ginseng flower extract is a repetitive pressurized and decompressed extract. 7. The method according to any one of claims 1 to 6,
Wherein the composition is a pharmaceutical composition. 7. The method according to any one of claims 1 to 6,
Wherein the composition is a cosmetic composition. 7. The method according to any one of claims 1 to 6,
Wherein the composition is a food composition. The method for producing a composition according to any one of claims 1 to 6,
A step of adding water or alcohol as a primary solvent to the ginseng flower, and then performing a primary extraction by repeatedly performing the pressure and decompression extraction steps. 11. The method according to claim 10,
Separating the extracted primary extract with a chlorine-containing organic solvent, removing the chlorine-containing organic solvent layer, and secondly extracting the water layer with an alcohol as a secondary solvent, wherein the primary solvent is an alcohol RTI ID = 0.0 > 1, < / RTI > the first extract is dissolved in water prior to fractionation. 11. The method of claim 10,
Characterized in that the pressurized and reduced pressure extraction step is repeated for a total of 18 to 30 hours to extract. 11. The method of claim 10,
Wherein the extraction temperature in the pressurized and reduced pressure extraction step is 25 to 35 占 폚. 11. The method of claim 10,
Wherein the pressure during the pressure extraction step is 60 to 100 MPa, and the pressure during the pressure extraction step is atmospheric pressure. 11. The method of claim 10, wherein the primary solvent is water. 12. The method of claim 11, wherein the chlorine-containing organic solvent is methylene chloride and the secondary solvent is ethylene. The method according to claim 1,
Wherein the composition is prepared by any one of claims 10 to 16.

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