KR20200144896A - Health functional food composition containing extract of Antirrhinum majus L. as an active ingredient for lowering blood glucose - Google Patents

Abstract

An extract of Antirrhinum majus of the present invention has an effect of reducing blood glucose concentration, and has an effect of alleviating glucose tolerance or insulin resistance, thereby being able to be usefully used for preventing, alleviating or treating diabetes, diabetic complications, hyperglycemia, impaired glucose tolerance, hyperinsulinemia, or insulin resistance syndrome.

Description

{Health functional food composition containing extract of Antirrhinum majus L. as an active ingredient for lowering blood glucose}

The present invention relates to a health functional food composition for lowering blood sugar containing a snapdragon extract as an active ingredient.

Diabetes is a type of metabolic disease caused by problems such as insufficient secretion of insulin from pancreatic cells or inability to function normally. Diabetes causes abnormal metabolic regulation in the body, so if proper treatment and management are not performed, the circulatory system It can cause back complications. Insulin is a peptide hormone with a molecular weight of about 5800, which is produced by the β cells of Langerhans islet in the pancreas through the precursor proinsulin. It is involved in sugar metabolism, amino acid metabolism, and lipid metabolism, and its typical physiological action is lowering blood sugar. Measurement of blood insulin concentration reflecting the insulin secretion function of β cells is a useful index for diagnosing diabetes, grasping the condition of disease, and discriminating the cause of abnormal glucose tolerance.

In diabetes, there is no problem with type 1 diabetes and insulin secretion, where insulin is not secreted properly due to impaired function of the pancreas beta cells, but there is no problem with insulin secretion due to poor insulin sensitivity. I have type 2 diabetes. In the case of type 1 diabetes, insulin treatment is required, in the case of type 2 diabetes, lifestyle correction is the basis, and drugs can be taken as an additional treatment.

In particular, type 2 diabetes, which accounts for 90% of all diabetic patients, is a disease caused by an increase in insulin receptor resistance or a decrease in insulin sensitivity, and is known to be caused by a combination of many environmental factors such as obesity, an increase in age, and lack of physical activity. Type 2 diabetes occurs frequently, especially in abdominal obesity. In addition, a person with type 2 diabetes does not absorb normal glucose, so the ability of glucose tolerance decreases, resulting in a state of hyperglycemia due to the continuous presence of sugar in the blood. The state of hyperglycemia is sorbitol in the tissues. ) Accumulates and protein glycation causes diabetic complications, and as a result, peripheral nerve disorders occur due to nerve damage and may progress to ulcers (eg, foot ulcers). Therefore, patients with diabetes have increased terminal vascular disease, impaired wound healing, and decreased defense ability against infection, making it difficult to adequately treat patients.

Diabetes mellitus increases the risk of cardiovascular complications by causing abnormalities in lipid metabolism as well as impaired glucose metabolism in the body. Since diabetes inducing causes are diverse, many studies are being conducted to develop new drugs based on each cause, and drugs taking complications into account are also being studied. However, drugs developed so far have been reported to have side effects such as hypoglycemia, loss of insulin secretion, gastrointestinal disorders, heart failure, and liver failure, and some drugs have been banned due to serious side effects. In order to solve the side effects of such drugs, the discovery of natural food-derived materials that can be used for a long time with few side effects has recently been attracting attention. Through this, treatments that can be treated alone or in combination with drugs and health for improvement and prevention The development of functional foods is also receiving a lot of attention.

Snapdragon ( Antirrhinum majus L.) is a perennial plant belonging to the Scrophulariaceae family. It is a perennial plant native to Africa and southern Europe, and is named because the flower shape looks like the mouth of a goldfish. In addition, snapdragon flowers are registered as a food ingredient managed by the Ministry of Food and Drug Safety and are edible, and are known to have anti-inflammatory, antioxidant, anti-inflammatory, and analgesic effects in the private sector.

As a study on snapdragon, Korean Patent Application Publication No. 10-2019-0054852 discloses the anti-inflammatory use of a pharmaceutical composition containing a snapdragon extract as an active ingredient, but directly discloses information on lowering blood sugar and preventing and improving diabetes. Not doing. Accordingly, in the present invention, the present invention was completed by confirming that the snapdragon extract has an effect of reducing blood glucose concentration and insulin resistance.

An object of the present invention is to provide a health functional food composition for lowering blood sugar.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating diabetes.

To achieve the above object,

One aspect of the present invention is snapdragon ( Antirrhinum It provides a health functional food composition for lowering blood sugar containing majus L.) extract as an active ingredient.

Another aspect of the present invention is Snapdragon ( Antirrhinum It provides a pharmaceutical composition for the prevention or treatment of diabetes containing majus L.) extract as an active ingredient.

Snapdragon extract of the present invention has the effect of reducing blood glucose (glucose) concentration, and has the effect of improving glucose tolerance or insulin resistance, so that diabetes, diabetic complications, hyperglycemia, impaired glucose tolerance, hyperinsulinemia or insulin resistance syndrome (insulin resistance syndrome) can be usefully used to prevent, improve, or treat.

Figure 1 is in the experimental group in which the snapdragon extract was administered to obesity-induced mice (HFAME, high fat diet and administered with Antirrhinum majus L. extract), the mouse experimental group (HFD, high fat diet) in which the snapdragon extract was not administered and obesity was induced. ) Than the mouse blood glucose (glucose) is a graph showing a further decrease.
FIG. 2 shows that in the experimental group in which the snapdragon extract was administered to obesity-induced mice (HFAME), the blood insulin concentration of the mice was further reduced in a concentration-dependent manner than the mouse experimental group (HFD) in which the snapdragon extract was not administered and obesity was induced. It is a graph showing that.
FIG. 3 shows HOMA-IR (Homeostatic model assessment-insulin resistance), which is an indicator of insulin resistance compared to the experimental group (HFD) in which the snapdragon extract was administered to obesity-induced mice (HFAME), ) This is a graph showing that the level decreases further depending on the concentration.
FIG. 4 shows that in the experimental group in which the snapdragon extract was administered to obesity-induced mice (HFAME), the blood glucose of the mice was further reduced than that of the mouse experimental group (HFD) in which obesity was induced without administration of the snapdragon extract, and glucose tolerance test (Glucose tolerance) test, GTT) is a graph showing that glucose tolerance was further improved when performing.
FIG. 5 shows that in the experimental group in which the snapdragon extract was administered to obesity-induced mice (HFAME), the blood glucose of the mice after insulin administration was further reduced after insulin administration than in the mouse experimental group (HFD) in which obesity was induced without administration of the snapdragon extract. It is a graph showing that insulin resistance was further improved when performing an insulin tolerance test (ITT).

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a health functional food composition for lowering blood sugar containing a snapdragon extract as an active ingredient.

The snapdragon extract can be prepared by a manufacturing method comprising the following steps:

1) preparing an extract by adding an extraction solvent to snapdragon;

2) filtering the extract of step 1); And

3) After concentrating the filtered extract of step 2) under reduced pressure, lyophilizing it to powder.

The snapdragon extract may be prepared by extracting with water, alcohol, or a mixture thereof.

The alcohol may be a C _1-3 lower alcohol, preferably methanol or ethanol.

The alcohol may be 30% to 99% ethanol, 40% to 90% ethanol, 50% to 80% ethanol, and 60% to 75% ethanol.

The extraction solvent may be added in an amount of 1 to 50 ml per 1 g of snapdragon used for extraction, and 5 to 40 ml per 1 g of snapdragon used for extraction. It may be added in an amount of 10 to 30 ml per 1 g by weight, and in an amount of 15 to 25 ml per 1 g by weight of snapdragon used for extraction.

The extraction method may be immersion, shaking extraction, Soxhlet extraction, or reflux extraction. At this time, the extraction time is 10 to 96 hours; 15 to 72 hours; 20 to 48 hours; Or 23 to 25 hours. The extraction may be repeated 1 to 5 times.

Meanwhile, the vacuum concentration in step 3) may be performed using a rotary vacuum concentrator, a vacuum vacuum concentrator, or a vacuum rotary evaporator. In addition, the drying may be vacuum drying, vacuum drying, boiling drying, spray drying or freeze drying, and specifically, freeze drying.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract obtained through another conventional extraction method, or an extract that has undergone purification and fermentation processes. Decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction by ultrasonic wave, separation by using an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatography, or natural or various microorganisms Active fractions obtained through various purification and extraction methods, such as an extract from the fermentation product used, are also included in the extract of the present invention.

The snapdragon may be any one or more selected from the group consisting of seeds, leaves, flowers, stems, and roots, and in one embodiment of the present invention, an extract was prepared using snapdragon flowers, but this is only an example and is not limited thereto. .

The snapdragon extract may reduce blood glucose or insulin concentration.

The snapdragon extract may reduce glucose tolerance or insulin resistance.

The snapdragon extract may be used for preventing, improving or treating diabetes.

The diabetes may be type 1 diabetes or type 2 diabetes.

Type 2 diabetes is characterized by insulin resistance. Insulin resistance is that the function of insulin to lower blood sugar is poor, so that cells cannot effectively burn glucose. When insulin resistance is high, blood sugar concentration increases, and sugar is excreted into the urine.

When the snapdragon extract was administered to obesity-induced mice, the blood glucose concentration of the mice was significantly lower. (See Fig. 1)

In addition, type 2 diabetes is often found in obese patients, that is, obesity due to a high fat diet accompanies insulin resistance, and the insulin resistance causes impairment of the influx of blood sugar into the cells (glucose tolerance), which is type 2 It causes high blood pressure, hyperlipidemia, and arteriosclerosis, which are found in diabetes.

Therefore, when performing a glucose tolerance test (GTT) indicating the degree of glucose tolerance, the experimental group in which the snapdragon extract was administered to the obesity-inducing mice than the mouse experimental group (HFD) in which obesity was induced without administration of the snapdragon extract ( HFAME) showed a significantly lower AUC (area under the glucose curve) value, and in particular, it was confirmed that the lowest AUC value was shown in the 600 mg/kg administration group. (See Fig. 4)

Insulin is secreted by the β cells of the pancreas and is a hormone that maintains a constant blood glucose level, which is the level of glucose in the blood. When blood sugar increases due to glucose after a meal, insulin is secreted from the β cells of the pancreas, and the secreted insulin promotes the absorption of glucose into the muscles. In addition, insulin suppresses the production of glucose in the liver and promotes glucose absorption into peripheral tissues, thereby lowering the blood glucose concentration. However, due to obesity, there is a problem in the insulin signaling system, and insulin cannot function properly, indicating insulin resistance, which prevents blood glucose control, and when insulin resistance increases, insulin secretion increases.

Therefore, in the experimental group (HFAME) in which the snapdragon extract was administered to obesity-inducing mice, the HOMA-IR (Homeostatic model assessment-insulin resistance), an indicator of insulin resistance, than the experimental group (HFD) of the mice inducing obesity without administration of the snapdragon extract. It was confirmed that the level was further decreased in a concentration-dependent manner (see FIG. 3), and in this Experimental Example 5-2, when performing an insulin tolerance test (ITT), an obesity was induced without administration of snapdragon extract. It was confirmed that in the experimental group (HFAME) in which the snapdragon extract was administered to obesity-inducing mice than the experimental group (HFD), in particular, the 600 mg/kg dose of snapdragon extract showed significantly lower AUC (area under the glucose curve) values, (See Fig. 5) In this Experimental Example 4, in the experimental group (HFAME) in which the snapdragon extract was administered to obesity-induced mice, the concentration of insulin in the blood of the mice was higher than that of the mouse experimental group (HFD) in which the snapdragon extract was not administered and obesity was induced. It was confirmed that it further decreased depending on the degree. (See Fig. 2)

Snapdragon extract according to the present invention has a hypoglycemic effect (see Experimental Example 2 and FIG. 1), an effect of improving glucose tolerance (see Experimental Examples 3 and 4), an effect of reducing blood insulin (see Experimental Examples 4 and 2), or reducing insulin resistance. It was confirmed through the following experimental examples that there was an effect (see Experimental Example 5, FIGS. 3 and 5), and the snapdragon extract was used for diabetes, diabetes complications, hyperglycemia, impaired glucose tolerance, hyperinsulinemia, or insulin resistance syndrome. It can be usefully used as a health functional food composition for preventing or improving, or lowering blood sugar.

The health functional food composition for lowering blood sugar containing the snapdragon extract of the present invention as an active ingredient may further contain one or more active ingredients exhibiting the same or similar function. For administration, it may additionally be prepared by including one or more carriers acceptable as food.

The health functional food composition for lowering blood sugar containing the snapdragon extract of the present invention as an active ingredient is preferably in any one formulation selected from beverages, pills, tablets, capsules, and powders, but is not limited thereto.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating diabetes containing a snapdragon extract as an active ingredient.

The pharmaceutical composition for preventing or treating diabetes containing the snapdragon extract of the present invention as an active ingredient contains 0.1 to 99.9% by weight of the extract of the present invention relative to the total weight of the composition as an active ingredient, and is pharmaceutically acceptable. Possible carriers, excipients or diluents may be included. The pharmaceutically acceptable carrier is not particularly limited as long as it is suitable for in vivo delivery of the composition, and is, for example, selected from the group consisting of saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol and ethanol. Any one of these components or one or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added as needed. In addition, a diluent, a dispersant, a surfactant, a binder, and a lubricant may be additionally added to form an injectable formulation such as an aqueous solution, suspension, emulsion, etc., a powder, tablet, capsule, pill, granule, or injection solution. Furthermore, it can be preferably formulated according to each disease or component using an appropriate method in the art.

In addition, the pharmaceutical composition for preventing or treating diabetes containing the snapdragon extract of the present invention as an active ingredient can be administered orally or parenterally, and when administered parenterally, subcutaneous injection, intravenous injection, intramuscular injection or intraperitoneal injection injection It is desirable to choose a method. In order to formulate a formulation for parenteral administration, the composition of the present invention is mixed with the composition in water to prepare a suspension, and it is formulated in an ampoule or vial unit dosage form.

The pharmaceutical composition for preventing or treating diabetes containing the snapdragon extract of the present invention as an active ingredient is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of patient's disease, severity, drug activity, Sensitivity to drugs, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field can be determined. The composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and this can be easily determined by a person skilled in the art.

Hereinafter, the present invention will be described in detail through examples and experimental examples.

However, the examples and experimental examples to be described later are only to specifically illustrate the present invention in one aspect, and the present invention is not limited thereto.

< Example 1> Snapdragon extract

Snapdragon, Antirrhinum used in the present invention majus L.) was purchased from Herb Five Me (Seoul, Korea). Snapdragon flowers are extracted with ethanol, specifically, 20L of 70% ethanol per 1 kg of snapdragon flowers are added and pulverized with a grinder (T25 Digital Ultra-Turrax Immersion Blender, 13326309, IKA, Staufen, Germany), and then at room temperature for 24 hours. During extraction. After the extract was filtered through a vacuum filtration device, the extraction solvent was removed and concentrated using a rotary vacuum concentrator (N-4000S, Eyela, Koishikawa, Japan). The concentrated extract was dried with a freeze dryer and powdered.

< Experimental example 1> Preparation of experimental animal model

Mice used in the following experimental examples were prepared as follows.

(1-1) Experimental animals and diet

As for the experimental animals, 40 6-week-old C57BL/6 male mice were purchased from Central Lab-Animal Inc., Seoul, Korea. The mice were adapted to a normal diet in which 10% of total calories were fat for one week, and then a normal diet group (ND, normal diet) according to the randomized complete block design; High fat diet group (HFD); And the powdered snapdragon extract prepared in Example 1 (AME, Antirrhinum majus L. extract) is administered in a suspension in sterile saline, and the final dose of the active ingredient of snapdragon extract is 100mg/kg (weight), 300mg/kg, and 600mg/kg, respectively, in a high fat diet and administered. with Antirrhinum majus L. extract); was classified into 5 experimental groups.

All experimental groups except the normal diet group fed a high-fat diet with 45% of total calories fat for 8 weeks to induce obesity, and the normal diet group supplied a regular diet with 10% of total calories fat. The experimental environment was kept at a constant temperature (22±3℃) and humidity (50±5℃), and was bred in an environment of a 12-hour light and dark cycle. The whole process of the experiment was carried out with the approval of the Animal Experimental Ethics Committee of the National Academy of Agricultural Sciences (NIAS201604).

(1-2) Experimental group selection and extract administration plan

The 40 mice were divided into a total of 5 experimental groups consisting of 8 mice per each experimental group, a normal diet group, a high fat diet group, and 3 kinds of snapdragon extract administration groups, and reared for 8 weeks. The final dose of the active ingredient of snapdragon extract is divided into 100mg/kg (body weight), 300mg/kg, and 600mg/kg respectively, and the powdered snapdragon extract prepared in Example 1 is sterile physiological saline. The suspension suspended by using was administered once a day for each group using sonde for oral administration for 8 weeks, and the standard diet group and the high fat diet group were orally administered the same amount of distilled water.

< Experimental example 2> Measurement of blood glucose (glucose) concentration

In order to confirm whether the snapdragon extract according to the present invention exhibits the effect of reducing blood glucose, the blood glucose concentration of the mouse prepared in Experimental Example 1 was measured using V-glucose (AM201, Asan Pharm. Co.), and the result Is shown in Figure 1.

In the experimental group administered with snapdragon extract to obesity-inducing mice (HFAME, high fat diet and administered with Antirrhinum majus L. extract), mice were compared with the experimental group (HFD, high fat diet) in mice in which obesity was induced without administration of snapdragon extract. Blood glucose concentration was significantly lower. (See Fig. 1)

The above results show that the high-fat diet group (HFAME) administered with the snapdragon extract decreased blood glucose concentration compared to the high-fat diet group (HFD) without administration of the snapdragon extract, the snapdragon extract has a blood sugar-lowering effect, and thus the snapdragon extract It can be seen that it can be used to prevent or improve diabetes, or to lower blood sugar.

< Experimental example 3> Glucose tolerance For analysis Glucose load test ( GTT , glucose tolerance test)

In order to check whether the snapdragon extract according to the present invention exhibits the effect of improving glucose tolerance, the mouse prepared in Experimental Example 1 was fasted for 12 hours before blood collection, and then a blood glucose meter (Acuu-check, Roche Diagnostics Korea Co., Ltd., Seoul) , Korea) was used to observe the change in each experimental group, and a glucose tolerance test (GTT) was performed at week 6, and a glucose solution was intraperitoneally injected into fasted mice at 1 g per 1 kg body weight, and then 0, 15 After 30, 60, 90 and 120 minutes, blood was collected from the tail vein of the mouse and blood glucose was measured. The area under the glucose curve (AUC) of the glucose tolerance test (GTT) was calculated in the range of 0 minutes to 120 minutes. The results are shown in FIG. 4.

When performing a glucose tolerance test (GTT) indicating the degree of glucose tolerance, the experimental group in which the snapdragon extract was administered to the obese-induced mice (HFAME), rather than the mouse experimental group (HFD) in which obesity was induced without administration of the snapdragon extract. Showed a significantly lower AUC (area under the glucose curve) value, especially the lowest AUC value in the 600 mg/kg administration group. (See Fig. 4)

The above results show that the high fat diet group (HFAME) administered with snapdragon extract compared to the high fat diet group (HFD) without administration of snapdragon extract improved glucose tolerance, which is the ability to metabolize glucose in vivo, resulting in a low AUC value in the glucose tolerance test. It can be seen that the snapdragon extract has an effect of improving glucose tolerance, and thus it can be seen that the snapdragon extract can be usefully used in preventing or improving diabetes, or lowering blood sugar.

< Experimental example 4> Measurement of insulin concentration in blood

In order to confirm whether the snapdragon extract according to the present invention exhibits a blood insulin reduction effect, the blood insulin concentration of the mouse prepared in Experimental Example 1 was immunized using an insulin ELISA kit (80-INSMS-E01, ALPCO, Seoul, Korea). It was measured by the immunoassay, and the results are shown in FIG. 2.

In the experimental group (HFAME) in which the snapdragon extract was administered to obesity-inducing mice, the concentration-dependent decrease in blood insulin concentration in the mice was more concentration-dependent than the experimental group (HFD) of the mice in which the snapdragon extract was not administered and obesity was induced. (See Fig. 2)

The above results show that the high-fat diet group (HFAME) administered with the snapdragon extract decreased the blood insulin concentration than the high-fat diet group (HFD) without administration of the snapdragon extract, indicating that the snapdragon extract has the effect of reducing the blood insulin concentration. Therefore, it can be seen that the snapdragon extract can be usefully used for preventing or improving diabetes, or lowering blood sugar.

< Experimental example 5> Analysis of insulin tolerance

In order to confirm whether the snapdragon extract according to the present invention exhibits the effect of reducing insulin resistance in which the function of insulin to lower blood sugar is lowered and cells cannot effectively burn glucose, the following experiment was performed on the mouse prepared in Experimental Example 1 Was performed, and the results are shown in FIGS. 3 and 5, respectively.

<Experimental Example 5-1> Measurement of HOMA-IR, an index of insulin resistance

The HOMA-IR method, which can be calculated by measuring fasting blood sugar and fasting insulin concentration, was used, which uses the principle that insulin secretion increases when insulin resistance increases, and insulin secretion decreases when insulin resistance decreases.

In the experimental group (HFAME) in which snapdragon extract was administered to obesity-induced mice, the level of HOMA-IR (Homeostatic model assessment-insulin resistance), an index of insulin resistance, was higher than that of the mouse experimental group (HFD) in which obesity was induced without administration of snapdragon extract. It decreased further depending on the concentration. (See Fig. 3)

The results show that the high-fat diet group (HFAME) administered with the snapdragon extract reduced the insulin resistance index HOMA-IR than the high-fat diet group (HFD) without the snapdragon extract administered, and the snapdragon extract reduced insulin resistance. In addition, it can be seen that there is a blood glucose reduction effect of Experimental Example 2 or blood insulin reduction effect of Experimental Example 4, and therefore, snapdragon extract can be usefully used for preventing or improving diabetes or lowering blood sugar. It can be seen that it proves.

<Experimental Example 5-2> Insulin tolerance test performed

After fasting the mice prepared in Experimental Example 1 for 12 hours before blood collection, changes in each experimental group were observed using a blood glucose meter (Acuu-check, Roche Diagnostics Korea Co., Ltd., Seoul, Korea). Insulin tolerance test (ITT) was performed at the 6th week, and the mice were intraperitoneally injected with an insulin solution of 1 unit per 1 kg of body weight, followed by blood from the tail vein of the mouse after 0, 15, 30, 60 and 90 minutes. Blood sugar was measured by collecting. The area under the glucose curve (AUC) of the insulin tolerance test (ITT) was calculated in the range of 0 minutes to 90 minutes.

When performing the insulin tolerance test (ITT), in the experimental group (HFAME) in which the snapdragon extract was administered to the obesity-induced mice, rather than the mouse experimental group (HFD) in which the snapdragon extract was not administered and the obesity was induced. The 600 mg/kg extract group showed significantly lower AUC (area under the glucose curve) values. (See Fig. 5)

The above results show that the high-fat diet group (HFAME) administered with snapdragon extract improved insulin resistance than the high-fat diet group (HFD) without administration of snapdragon extract, and thus showed a low AUC value in ITT. It can be seen that there is an effect of reducing, and furthermore, the effect of reducing blood glucose in Experimental Example 2 or the effect of reducing blood insulin in Experimental Example 4, and therefore, the snapdragon extract is useful for preventing or improving diabetes, or lowering blood sugar. It can be seen that it proves that it can be used.

<Statistics processing>

All experimental values in the above experimental examples were expressed as mean±standard error (mean±SEM), and statistical comparative analysis was performed using GraphPad Prism 5 Software (San Diego, CA, USA). For the difference between each group, Student's t-test analysis was used, and at this time, it was judged that there was a significant difference when the P value was less than 0.05.

In FIGS. 1 to 5, the data were provided as mean ± standard error of the mean (SEM), and ^*** P<0.001 compared to the normal diet group, ^# P<0.05, ^## P<0.01, ^{# ##} P<0.001 compared to the high fat diet.

Meanwhile, the pharmaceutical composition according to the present invention can be formulated in various forms depending on the purpose. The following illustrates some formulation methods containing the pharmaceutical composition according to the present invention as an active ingredient, but the present invention is not limited thereto.

<Formulation Example 1> Preparation of pharmaceutical formulation

1-1. Preparation of powder

500 mg of snapdragon extract of the present invention

100 mg lactose

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

1-2. Manufacture of tablets

500 mg of snapdragon extract of the present invention

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet preparation method.

1-3. Preparation of capsules

500 mg of snapdragon extract of the present invention

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare a capsule.

1-4. Preparation of injections

500 mg of snapdragon extract of the present invention

Suitable amount of sterile distilled water for injection

proper amount of pH adjuster

It is prepared with the above ingredients per ample (2 ml) according to a conventional injection preparation method.

1-5. Preparation of liquid

100 mg of snapdragon extract of the present invention

10 g of isomerized sugar

5 g of mannitol

Purified water appropriate amount

According to the usual preparation method of liquid preparation, add and dissolve each component in purified water, add lemon scent, mix the above ingredients, add purified water, add purified water, adjust the total to 100 ml, and fill in a brown bottle. The liquid is prepared by sterilization.

As described above, the present invention has been described in detail through preferred embodiments and experimental examples, but the scope of the present invention is not limited to specific examples, and should be interpreted by the appended claims. In addition, those who have acquired ordinary knowledge in this technical field should understand that many modifications and variations can be made without departing from the scope of the present invention.

Claims (7)

Snapdragon ( Antirrhinum A health functional food composition for lowering blood sugar containing majus L.) extract as an active ingredient.
The method of claim 1,
The health functional food composition, characterized in that the lowering of blood sugar decreases glucose tolerance or insulin resistance.
The method of claim 1,
The lowering of blood sugar is a health functional food composition for preventing or improving diabetes, diabetes complications, hyperglycemia, impaired glucose tolerance, hyperinsulinemia or insulin resistance syndrome.
The method of claim 1,
The snapdragon extract is a health functional food composition, characterized in that produced by extracting with water, alcohol or a mixture thereof.
The method of claim 1,
The snapdragon is a health functional food composition, characterized in that at least one selected from the group consisting of seeds, leaves, flowers, stems and roots.
Snapdragon ( Antirrhinum majus L.) A pharmaceutical composition for preventing or treating diabetes containing an extract as an active ingredient.
The method of claim 6,
The pharmaceutical composition, characterized in that the diabetes is type 2 diabetes.

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