KR100979882B1 - Compositions for preventing and curing diabetes comprising extracts of Lonicera japonica and Panax ginseng C.A. Meyer - Google Patents

Abstract

The present invention relates to a composition for the prevention and treatment of diabetes mellitus extract and extract containing red ginseng extract, more specifically preventing the absorption of diabetes by lowering blood sugar by inhibiting the absorption of sugar by containing the extract extracted with gold silver and red ginseng as an active ingredient It relates to a composition having a therapeutic effect.

According to the present invention, it was confirmed that the gold and silver ginseng extract and red ginseng extract can be effectively used for diabetes by inhibiting sugar absorption, respectively. In addition, it was confirmed that the mixed composition using the gold and silver ginseng extract and red ginseng extract has a significant synergistic effect compared with the administration of the gold and silver ginseng extract alone, and thus can be used as an antidiabetic material. have.

Gold, silver ginseng, extract, anti-diabetic, diabetes

Description

Compositions for preventing and treating diabetes comprising gold silver flower extract and red ginseng extract {Compositions for preventing and curing diabetes comprising extracts of Lonicera japonica and Panax ginseng C.A. Meyer}

The present invention relates to a composition for the prevention and treatment of diabetes mellitus extract and extract containing red ginseng extract, more specifically preventing the absorption of diabetes by lowering blood sugar by inhibiting the absorption of sugar by containing the extract extracted with gold silver and red ginseng as an active ingredient It relates to a composition having a therapeutic effect.

Diabetes is a metabolic disease that causes abnormalities in glucose metabolism due to insulin deficiency or insulin resistance. If diabetes lasts for many years, it can cause complications such as glaucoma, blindness, autonomic neuropathy, kidney failure, angina pectoris, myocardial infarction, high blood pressure and stroke. Diabetes has insulin-dependent, type 1 diabetes, in which the beta cells of the pancreas that secrete insulin are destroyed, leading to diabetes, and beta cells have insulin-independent, type 2 diabetes, which is caused by normal or insulin receptor resistance. , Glucose tolerance, and gestational diabetes, which are inefficient insulin secretion. In modern society, obesity due to changes in eating habits has risen sharply, and type 2 diabetes patients have increased rapidly due to insulin resistance due to obesity. Therefore, in order to find an effective material for both type 1 and type 2 diabetes, we searched for a material that effectively suppresses the absorption of sugar in plant material.

Gold and silver coins used in the present invention is a flower of Indongcho ( Lonicera japonica ) has an antipyretic action and detoxification effect is used for various boils and fever diseases, etc. The plant has been used mainly as a medicine by medicinal effects, pharmacological effects. Indongcho is a vine that grows frequently in the mountains and fields, including forest edges around the country, and is a semi-hepatic vine plant of Indongaceae that survives cold winters.

On the other hand, the biggest problem of the modern man is a disease such as diabetes due to obesity, and in order to prevent or treat this disease, it is most ideal to suppress the absorption of the carbohydrates and the carbohydrates in the small intestine when eating carbohydrates. In Korea, only extracts such as bundling or red ginseng are sold as functional foods.However, by using a single herbal medicine, not only can you expect a certain function by a single herbal medicine, but also you cannot secure the disadvantages of a single herbal medicine. there was.

Accordingly, the present inventors have made intensive research to overcome the problems of the prior art, it was confirmed that the extract of gold and silver coins effectively inhibit the absorption of sugar, in particular, the synergistic effect in the mixture with the red ginseng extract, and the present invention To complete.

Therefore, the main object of the present invention is to provide a composition having the effect of preventing and treating diabetes by inhibiting the absorption of sugar by containing the extract extracted gold and silver ginseng as alcohol as an active ingredient.

According to an aspect of the present invention, the present invention provides a composition for preventing and treating diabetes through lowering blood sugar, which contains a gold and silver extract as an active ingredient.

In existing studies of anti-diabetic, it is known that gold and silver coins have anti-diabetic effects with dozens of herbal drugs (for example, Korean Patent Publication No. 10-2005-0028272; diet, anti-diabetic, hyperlipidemia and antioxidants). There is only a domestic patent (Publication Patent No. 10-2005-0005630) for a composition having a therapeutic effect on diabetes alone with a functional food using an effective complex prescription) and a sterling extract alone. Therefore, the present inventors have confirmed that the sugar absorption inhibitory effect by using the gold silver extract, red ginseng extract, and mixtures thereof, and in particular, when administering the mixture of gold silver extract and red ginseng extract alone. When the inventors first studied that the blood sugar concentration can be lowered more synergistically, the inventors confirmed the effect.

In the composition of the present invention, the mixing ratio of the sterling silver extract and the red ginseng extract is preferably 1: 2 to 2: 1 weight ratio, more preferably 1: 1 weight ratio. As can be seen from the embodiment of the present invention and Figure 1, the gold and silver extract of the present invention can lower the blood sugar concentration in a concentration-dependent manner, the mixture of red ginseng extract and 1: 1 weight ratio is more than using the gold and silver extract alone It was confirmed that blood sugar levels can be effectively lowered (see Table 2).

In the composition of the present invention, the sterling silver and red ginseng may be extracted with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably ethanol (alcohol) is extracted by diluting in water to 30-90% It is suitable to do. As used herein, the term alcohol refers to edible ethanol as used in the art.

Extraction using the spirit is generally the most commonly used solvent for the extraction of physiologically active substances in plants, as well as easy to extract a variety of components, it is convenient for separation and purification after extraction. In addition, the alcohol extract is concentrated immediately, and lyophilized to obtain an extract dried powder of the plant.

As a specific method of extracting the extracts of the present invention, first, the gold and silver extract is preferably extracted for 2-6 hours with 30-70% alcohol and more preferably for 3-5 hours with 40-60% alcohol. In addition, the red ginseng extract is preferably extracted for 6-10 hours with 30-70% alcohol and more preferably for 7-9 hours with 40-60% alcohol. If the extraction time is less than the above range, the active ingredient having a sugar absorption inhibiting effect may not be properly extracted. On the contrary, if the extraction time is more than the above range, the extraction yield of the active ingredient does not increase any more, resulting in inferior economic efficiency in the production process. .

In the present invention, the composition may be prepared by a method known in the pharmaceutical field to be used as a pharmaceutical composition, and may be mixed with itself or a pharmaceutically acceptable carrier, excipient, diluent, etc. to powder, granule, tablet, It may be prepared and used in formulations such as capsules or injections. They can also be administered orally or parenterally.

The dosage for administering the mixture of the sterling silver extract and the red ginseng extract according to the present invention as an active ingredient may be appropriately selected according to the age, sex, condition and symptoms of the disease of the patient, preferably 0.1 to 1000 mg / kg per day. Mixtures of (body weight) may be administered.

According to another aspect of the present invention, the present invention provides a dietary supplement for preventing and treating diabetes, which contains a sterling extract and a red ginseng extract as active ingredients.

In the present invention, "health functional food" refers to a food having a bioregulatory function, such as prevention and treatment of diseases, biological defense, immunity, recovery of symptoms, inhibition of aging, and should be harmless to the human body when taken in the long term.

In order to be used as a dietary supplement for the prevention and treatment of diabetes mellitus extract and red ginseng extract of the present invention, it can be prepared by a variety of methods known in the food science or pharmaceutical field, or is acceptable It can be prepared in any food form which can be ingested orally by mixing with a carrier, excipient, diluent, and the like. Preferably in the form of beverages, pills, granules, tablets or capsules.

The health functional food of the present invention may further include food supplements which are commonly added in food production and are food acceptable. For example, when the beverage is prepared, in addition to the plant extract of the present invention, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and the like may be further included.

The amount that can be included as an active ingredient of the health functional food according to the present invention may be appropriately selected according to the age, sex, weight, condition, symptoms of the disease of the person who wants to prevent and treat diabetes, preferably 0.01 days per adult It is good to include from g to 10.0g, by ingesting a health functional food having such a content can be obtained anti-diabetic effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Preparation of Gold Coin Extract

500 g of gold and silver coins (dry weight; sun dried gold and silver coins purchased from Daejeon Pharmaceutical Market) were extracted in 6L 50% alcohol for 1 hour and 2 times at 65 ° C for 4 hours, centrifuged at 5,000 rpm for 20 minutes, Concentration under reduced pressure afforded 190.3 g of extract. This extract was used as a sample of the gold and silver extract extract, wherein the water content was 32.2%.

Example 2. Preparation of Red Ginseng Extract

50% alcohol is added to raw red ginseng (jasam ginseng or Jungmi ginseng; Korea Ginseng Corporation) 5-6 times the raw red ginseng and extracted for 8 hours at about 60-70 ℃. The extract is cooled and then filtered to remove sludge. This filtrate was prepared by concentration of 62-74 ° Bx (this unit represents sugar, and the filtrate was concentrated until it reached 62-74 ° Bx) (43% of water content). It was. This extract was used as a sample of red ginseng extract.

Example 3. Preparation of Gold Silver and Red Ginseng Mixture

Using the extract samples prepared in Examples 1 and 2 were mixed in a 1: 1 (weight ratio), respectively, to prepare a mixed sample of the gold and silver extract and red ginseng extract.

Example 4 Glucose Tolerance Test of Gold Silver and Red Ginseng Extract and Mixture

The experimental animals were SD (Sprague-Dawley) rats (220-250g, 7 weeks old, KOATEC Co., Ltd.) male, the test group to the samples prepared in Examples 1, 2 and 3 in 0.8ml physiological saline Each 25 mg (100 mg / kg body weight) was dissolved, or 125 mg (500 mg / kg body weight) was dissolved in 0.8 ml of saline solution was prepared in Example 1, was used as shown in Table 1.

Table 1 Test group and sample dose for glucose tolerance test

Before the start of the experiment, fasted for 24 hours, and the samples were first orally pre-administered, and after 10 minutes, the mixture was co-administered with glucose (0.5 mg). The control group was administered with 0.8 ml of water and glucose was mixed with the sample to be 2 mg / kg. At this time, the administration volume was the same as 0.8ml. After simultaneous administration of glucose and sample, blood was collected from the tail vein and blood glucose was measured at 0, 30, 60, 90, and 120 minutes (FIGS. 1 and 2).

In Figures 1 and 2, the sterling silver extract lowered the blood glucose concentration in a concentration-dependent manner. In particular, at 500 mg / kg, blood glucose levels decreased by 22.5% and 24.5% at 30 and 60 minutes, respectively. In addition, 100 mg / kg of the sample mixed with gold and silver ginseng extract and 1: 1 (weight ratio), respectively, and when administered red ginseng and gold and silver ginseng at 60 minutes, respectively, 5.8% and 1.5% respectively. Although there was a reduction effect, the mixture showed a statistically significant synergistic effect with a decrease of 18.8%, which is much lower than the arithmetic mean of the two components (3.2%). At 90 minutes, red ginseng and gold and silver were respectively decreased by 23.4% and 26.6%, but in the mixture, 37.8%, which was significantly lower than the arithmetic mean (25.0%) of the two components, was statistically significant. Seemed.

In addition, at 120 minutes, red ginseng and sterling silver were reduced by 15.7% and 23.7%, respectively, but in the mixture, a significant decrease of 45.5%, which was significantly lower than the arithmetic mean (19.7%), was statistically significant. It showed an effect. These results are summarized in Table 2 below.

[Table 2] Blood glucose level (%) compared to the control

*: P <0.05, **: P <0.01

Preparation Example 1 Drink Containing Gold Coin and Red Ginseng Extract

1000 mg gold and silver ginseng extract 1000 mg and red ginseng extract 75 mg, vitamin C 75 mg, vitamin B2 4 mg, vitamin B6 3 mg, dietary fiber 1,000 mg, liquid fructose 20000 mg, emulsifier 100 mg, fragrance 50 mg prepared in Examples 1 and 2 50 Mix purified water to ml. The final mixed solution clarified by passing the mixed solution through a filter of 2-3 μm was pre-sterilized at 90-93 ° C. for 15-20 seconds, filled into a 50 ml bottle, and sterilized after 15-20 minutes at 80-85 ° C. Completed.

Preparation Example 2 Tablets Containing Gold Silver and Red Ginseng Extract

1000 mg of gold and silver ginseng extract prepared in Examples 1 and 2 and 1000 mg of red ginseng extract were mixed with 75 mg of vitamin C, 4 mg of vitamin B2, 3 mg of vitamin B6, and 100 mg of dietary fiber, and then 200 mg of lactose and 260 mg of lactose as excipients. 30 mg of the fragrance was mixed and kneaded to prepare granules through a granulation machine. Then, 5 mg of magnesium stearate was mixed and evenly mixed, and then compressed into a certain weight to complete the tablet product.

As described above, according to the present invention it can be seen that the gold silver extract can be effectively used for diabetes by inhibiting sugar absorption. In particular, it was confirmed that the mixed composition of the gold silver extract and red ginseng extract has a synergistic effect when compared with the administration of gold silver extract and red ginseng extract alone. Therefore, since there is no side effect and can be used for both type 1 and type 2 diabetes, the use as an antidiabetic material in the food and pharmaceutical industry is expected to increase.

1 is a glucose tolerance test results according to the gold silver extract.

Figure 2 is a glucose tolerance test results according to the gold silver extract, red ginseng extract and mixtures thereof.

Claims (6)

It contains a mixture of gold and silver ginseng extract and red ginseng extract in a 1: 1 weight ratio as the active ingredient, the gold and silver extract is extracted with 30-70% alcohol for 2-6 hours and the red ginseng extract is 6- with 30-70% alcohol A composition for preventing and treating diabetes, which is prepared by extracting for 10 hours. delete delete delete delete It contains a mixture of gold and silver ginseng extract and red ginseng extract in a 1: 1 weight ratio as the active ingredient, the gold and silver extract is extracted with 30-70% alcohol for 2-6 hours and the red ginseng extract is 6- with 30-70% alcohol Health functional foods for preventing and improving diabetes, characterized in that produced by extracting for 10 hours.

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