KR20200143294A - 다양한 남세균 유래 프로토포르피리노겐 ix 옥시다아제 또는 이의 변이체를 이용하는 제초제 내성 부여 및/또는 증진을 위한 조성물 및 방법 - Google Patents
다양한 남세균 유래 프로토포르피리노겐 ix 옥시다아제 또는 이의 변이체를 이용하는 제초제 내성 부여 및/또는 증진을 위한 조성물 및 방법 Download PDFInfo
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Abstract
다양한 생물에서 유래한 프로토포르피리노겐 IX 옥시다아제(Protoporphyrinogen IX oxidase) 또는 이의 아미노산 변이체, 및 이들의 식물 및/또는 조류(algae)의 제초제에 대한 내성을 부여하거나 및/또는 내성을 보다 증진시키기 위한 용도가 제공된다.
Description
다양한 생물에서 유래한 프로토포르피리노겐 IX 옥시다아제 (Protoporphyrinogen IX oxidase) 또는 이의 변이체, 및 이들의 식물 및/또는 조류 (Algae)의 제초제에 대한 내성을 부여 및/또는 증진시키기 위한 용도가 제공된다.
포르피린 합성 경로는 식물의 대사 과정에서 중요한 엽록소와 헴 (Heme)을 합성하는 과정으로 엽록체에서 이루어진다. 이 경로에서, 프로토포르피리노겐 IX 옥시다아제 (Protoporphyrinogen IX oxidase, 이하 PPO; EC:1.3.3.4)는 프로토포르피리노겐 IX (Protoporphyrinogen IX)로부터 프로토포르피린 IX (Protoporphyrin IX)로의 산화과정을 촉매한다. 프로토포르피리노겐 IX이 프로토포르피린 IX로 산화된 후 Mg-chelatase에 의해 마그네슘과 결합하면 엽록소가 합성 되고, Fe-chelatase에 의해 철과 결합하면 헴 (Heme)이 합성된다.
따라서, PPO의 활성이 저해되면 엽록소와 헴의 합성이 억제되고, 기질인 프로토포르피리노겐 IX이 정상적인 포르피린 합성 경로에서 이탈하여 엽록체에서 세포질로 이동하여 빠르게 프로토포르피린 IX으로 산화되면, 광과 산소 분자의 존재 하에서 활성이 높은 단일항산소 (singlet oxygen, 1O2)를 발생시켜 식물의 세포막을 파괴함으로써 급속한 식물 세포 사멸을 일으킨다. 이러한 원리를 이용하여, PPO 활성을 억제하는 제초제가 개발되었으며, 현재까지 화학 구조에 따라 피리미딘디온계 (pyrimidinediones), 디페닐에테르계 (diphenyl-ethers), 페닐피라졸계 (phenylpyrazoles), 페닐프탈이미드계 (N-phenylphthalimides), 티아디아졸계 (thiadiazoles), 옥사디아졸계 (oxadiazoles), 트리아지논계 (triazinones), 트리아졸리논계 (triazolinones), 옥사졸리딘디온계 (oxazolidinediones) 및 기타의 10개 계통의 제초제가 사용되고 있다.
또한, 상기 제초제들을 사용하면서도 재배 대상 작물의 생장에는 영향을 미치지 않게 하기 위해서는 재배 대상 작물에 상기 제초제들에 대한 내성을 부여할 필요성이 대두되어 왔다.
한편, 조류 (Algae)는 광합성 생물로서, 빛에너지를 다양한 유용 화합물을 합성할 수 있는 에너지로 전환할 수 있다. 예를 들어, 조류는 광합성에 의하여 탄소를 고정시키며, 이산화탄소를 당, 전분, 지질, 지방 또는 다른 생체분자들로 전환함으로써, 대기 중의 온실가스를 제거할 수 있다. 또한, 조류의 대규모 배양을 통하여 산업적 효소, 치료 화합물 및 단백질, 영양 물질, 상업적 물질, 연료 물질과 같은 다양한 물질들을 생산할 수 있다.
그러나, 생물반응기 (bioreactor) 또는 공개된 또는 폐쇄된 연못에서 조류를 대규모로 배양하는 경우, 원하지 않는 경쟁 생물들, 예를 들어 원치 않는 조류, 곰팡이, 윤충류 (rotifer) 또는 동물성 플랑크톤들에 의하여 오염이 발생할 수 있다.
따라서, 원하는 식물 및/또는 조류에 제초제 내성을 부여한 후, 제초제 내성이 없는 경쟁 생물들의 성장을 저해할 수 있는 농도로 제초제를 처리하여, 원하는 식물 및/또는 조류를 대규모로 수확할 수 있는 기술이 필요하다.
본 명세서에서, 원핵생물로부터 hemY 타입의 PPO 유전자를 분리하여 이 유전자 및 이의 아미노산 변이체가 프로토포르피리노겐 IX 옥시다아제 (PPO) 활성 저해 제초제들에 대하여 광범위한 제초제 내성을 나타냄이 입증되어, 이를 식물 및/또는 조류 (Algae)에 발현시키면 제초제 내성을 부여하거나 및/또는 내성을 증진시킬 수 있음이 제안된다.
일 예는 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열을 포함하는 폴리펩타이드를 제공한다.
다른 예는 PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 1의 아미노산들 (예컨대, 서열번호 1의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 해당 위치의 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 1의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체를 제공한다. 상기 서열번호 1의 폴리펩타이드의 PPO 활성 저해 제초제와의 상호작용에 관여하는 아미노산들로 이루어진 군에서 선택된 하나 이상은 서열번호 1의 아미노산 서열 중 N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상일 수 있다.
다른 예는 PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 2의 아미노산들 (예컨대, 서열번호 2의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 2의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체를 제공한다. 상기 서열번호 2의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들로 이루어진 군에서 선택된 하나 이상은 서열번호 2의 아미노산 서열 중 N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상일 수 있다.
다른 예는 PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 3의 아미노산들 (예컨대, 서열번호 3의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 3의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체를 제공한다. 상기 서열번호 3의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들로 이루어진 군에서 선택된 하나 이상은 서열번호 3의 아미노산 서열 중 N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상일 수 있다.
다른 예는 상기 폴리펩타이드 또는 폴리펩타이드 변이체를 암호화하는 폴리뉴클레오타이드를 제공한다.
다른 예는 상기 폴리뉴클레오타이드를 포함하는 재조합 벡터를 제공한다. 상기 재조합 벡터는 상기 폴리뉴클레오타이드를 적절한 숙주세포에서 발현시키기 위한 발현 벡터로 사용될 수 있다.
다른 예는 상기 재조합 벡터를 포함하는 재조합 세포를 제공한다.
다른 예는
(1) 서열번호 1의 폴리펩타이드, 서열번호 1의 폴리펩타이드의 변이체, 서열번호 2의 폴리펩타이드, 서열번호 2의 폴리펩타이드의 변이체, 서열번호 3의 폴리펩타이드, 서열번호 3의 폴리펩타이드의 변이체, 및 이들과 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드 또는 폴리펩타이드 변이체;
(2) 상기 (1)의 폴리펩타이드 또는 폴리펩타이드 변이체를 암호화하는 폴리뉴클레오타이드;
(3) 상기 (2)의 폴리뉴클레오타이드를 포함하는 재조합 벡터; 및
(4) 상기 (3)의 재조합 벡터를 포함하는 재조합 세포
로 이루어진 군에서 선택된 하나 이상을 포함하는, 식물 및/또는 조류의 제초제에 대한 내성 부여 및/또는 증진용 조성물을 제공한다. 일 예에서 상기 서열번호 1의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 80의 핵산 서열을 포함하는 것일 수 있고, 서열번호 2의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 81의 핵산 서열을 포함하는 것일 수 있으며, 서열번호 3의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 82의 핵산 서열을 포함하는 것일 수 있으나, 이에 제한되는 것은 아니고, codon degeneracy에 따라 상기 아미노산 서열을 암호화하는 다양한 핵산 서열을 포함할 수 있다.
상기 제초제는 프로토포르피리노겐 IX 옥시다아제 활성을 억제하는 제초제일 수 있다.
구체 예로, 상기 제초제는 피리미딘디온계 (pyrimidinediones), 디페닐에테르계 (diphenyl-ethers), 페닐피라졸계 (phenylpyrazoles), 페닐프탈이미드계 (N-phenylphthalimides), 페닐에스테르계 (phenylesters), 티아디아졸계 (thiadiazoles), 옥사디아졸계 (oxadiazoles), 트리아지논계 (triazinones), 트리아졸리논계 (triazolinones), 옥사졸리딘디온계 (oxazolidinediones) 및 기타 제초제로 이루어진 군에서 선택되는 1종 이상일 수 있으나, 이에 제한되는 것은 아니다.
구체 예로, 상기 제초제는 티아페나실 (tiafenacil), 부타페나실 (butafenacil), 사플루페나실 (saflufenacil), 벤즈펜디존 (benzfendizone), 포메사펜 (fomesafen), 옥시플루오르펜 (oxyfluorfen), 아클로니펜 (aclonifen), 아시플루오르펜 (acifluorfen), 비페녹스 (bifenox), 에톡시펜 (ethoxyfen), 락토펜 (lactofen), 클로메톡시펜 (chlomethoxyfen), 클로르니트로펜 (chlornitrofen), 플루오로글리코펜-에틸 (fluoroglycofen-ethyl), 할로사펜 (halosafen), 피라플루펜-에틸 (pyraflufen-ethyl), 플루아졸레이트 (fluazolate), 플루미옥사진 (flumioxazin), 시니돈-에틸 (cinidon-ethyl), 플루미클로락-펜틸 (flumiclorac-pentyl), 플루티아셋 (fluthiacet), 티디아지민 (thidiazimin), 옥사디아길 (oxadiargyl), 옥사디아존 (oxadiazon), 카펜트라존 (carfentrazone), 설펜트라존 (sulfentrazone), 트리플루디목사진 (trifludimoxazin), 아자페니딘 (azafenidin), 펜톡사존 (pentoxazone), 피라클로닐 (pyraclonil), 플루펜피르-에틸 (flufenpyr-ethyl), 프로플루아졸 (profluazol), 페노필레이트 (phenopylate; 2,4-dichlorophenyl 1-pyrrolidinecarboxylate), 페노필레이트의 카바메이트 유사체 (예컨대, O-phenylpyrrolidino- and piperidinocarbamate analoges (“Ujjana B. Nandihalli, Mary V. Duke, Stephen O. Duke, Relationships between molecular properties and biological activities of O-phenyl pyrrolidino- and piperidinocarbamate herbicides., J. Agric. Food Chem., 40(10) 1993-2000, 1992” 참조)), 이들의 농약적으로 허용가능한 염들, 및 이들의 조합으로 이루어진 군에서 선택되는 1종 이상일 수 있으나, 이에 제한되는 것은 아니다.
상기 식물은 광합성을 하는 다세포 진핵 생물을 의미하는 것으로, 단자엽 식물 또는 쌍자엽 식물이거나, 초본 식물 또는 목본 식물일 수 있다. 상기 조류 (Algae)는 광합성을 하는 생물로 진핵 (Eukaryotic) 조류이다.
일 예에서, 상기 식물 또는 조류는 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하도록 유전적으로 조작되며 상기 제2의 제초제에 대한 더 넓은 범위의 제초제 내성이 부여되거나 및/또는 증진된 것일 수 있다. 상기 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하도록 유전적으로 조작된 식물 또는 조류는 상기한 제초제에 대한 내성 부여 및/또는 증진용 조성물에 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함시킨 것을 사용하여 제조된 것일 수 있다. 따라서, 제초제에 대한 내성 부여 및/또는 증진용 조성물은 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하는 것일 수 있다.
구체 예로, 상기 제2의 제초제는 세포분열 저해성 제초제, 광합성 저해성 제초제, 아미노산 합성 저해성 제초제, 색소체 저해성 제초제 및 세포막 저해성 제초제를 포함하나, 이에 제한되는 것은 아니다.
구체 예로, 상기 제2의 제초제는 글리포세이트 (glyphosate), 글루포시네이트 (glufosinate), 디캄바 (dicamba), 2,4-D (2,4-Dichlorophenoxyacetic acid), 이속사플루톨 (isoxaflutole), ALS (acetolactate synthase) 저해성 제초제, 제2광계 (photosystem II) 저해성 제초제, 페닐우레아 (phenylurea)계 제초제 또는 브로목시닐 (bromoxynil)계 제초제 또는 이들의 조합을 예시할 수 있으나, 이에 제한되는 것은 아니다.
구체 예로, 제2의 제초제 내성 폴리펩타이드는 글리포세이트 제초제 내성 EPSPS (glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase), GOX (glyphosate oxidase), GAT (glyphosate-N-acetyltransferase) 또는 글리포세이트 디카복실레이즈 (glyphosate decarboxylase); 글루포시네이트 제초제 내성 PAT (phosphinothricin-N-acetyltransferase); 디캄바 제초제 내성 DMO (dicamba monooxygenase); 2,4-D 제초제 내성 2,4-D 모노옥시게나아제 또는 AAD (aryloxyalkanoate dioxygenase); ALS 저해성 설포닐우레아 (sulfonylurea)계 제초제 내성 ALS (acetolactate synthase), AHAS (acetohydroxyacid synthase), 또는 AtAHASL (Arabidopsis acetohydroxyacid synthase large subunit); 제2광계 저해성 제초제 내성 광계 II (photosystem II) 단백질 D1; 페닐우레아계 제초제 내성 시토크롬 P450 (cytochrome P450); 색소체 저해성 제초제 내성 HPPD (hydroxyphenylpyruvate dioxygenase); 브로목시닐 제초제 내성 니트릴레이즈 (nitrilase); 및 이들의 조합으로 이루어진 군에서 선택되는 1종 이상을 예시할 수 있으나, 이에 제한되는 것은 아니다.
또한, 상기 제2의 제초제 내성 폴리펩타이드를 암호화하는 유전자는 글리포세이트 제초제 내성 cp4 epsps, epsps (AG), mepsps, 2mepsps, goxv247, gat4601 또는 gat4621 유전자; 글루포시네이트 제초제 내성 bar, pat 또는 pat (SYN) 유전자; 디캄바 제초제 내성 dmo 유전자; 2,4-D 제초제 내성 AAD-1, AAD-12 유전자; ALS 저해성 설포닐우레아계 제초제 내성 ALS, GM-HRA, S4-HRA, ZM-HRA, Csr1, Csr1-1, Csr1-2, SurA 또는 SurB 유전자; 제2광계 (photosystem II) 저해성 제초제 내성 psbA 유전자; 페닐우레아 제초제 내성 CYP76B1 유전자; 이속사플루톨 제초제 내성 HPPDPF W336 유전자 및 브로목시닐 제초제 내성 bxn 유전자; 및 이들의 조합으로 이루어진 군에서 선택되는 1종 이상을 예시할 수 있으나, 이에 제한되는 것은 아니다.
다른 예는 상기 폴리뉴클레오타이드로 형질전환된, 제초제 내성 식물 및/또는 조류의 제초제에 대한 내성이 부여 및/또는 증진된 형질전환체, 이의 클론, 또는 자손을 제공한다.
다른 예는 상기 폴리뉴클레오타이드를 식물 및/또는 조류에 형질전환하는 단계를 포함하는, 제초제에 대한 내성이 부여 및/또는 증진된 식물 또는 조류를 제조하는 방법을 제공한다.
다른 예는 상기 폴리뉴클레오타이드를 식물 및/또는 조류에 형질전환하는 단계를 포함하는, 식물 및/또는 조류의 제초제에 대한 내성을 부여 및/또는 증진시키는 방법을 제공한다.
상기 형질 전환은 조류, 및/또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초, 또는 전체 (whole body)에 대하여 수행되는 것일 수 있다.
상기 형질전환체는 조류, 또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초, 또는 전체 (whole body) 일 수 있다. 상기 형질전환체는 최초 형질전환체(0대)로부터 얻어진 자손 (예컨대, 1~8대 자손)을 포함할 수 있다.
다른 예는, 상기 폴리펩타이드, 폴리펩타이드 변이체, 이를 암호화하는 폴리뉴클레오타이드, 상기 폴리뉴클레오타이드를 포함하는 재조합 벡터, 및 상기 재조합 벡터를 포함하는 재조합 세포로 이루어진 군에서 선택된 하나 이상을 포함하는 군에서 선택되는 1종 이상을 포함하는 식물을 재배지에 제공하는 단계, 및 상기 재배지(또는 상기 식물)에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계를 포함하는, 재배지에서 잡초를 방제하는 방법을 제공한다.
구체 예로, 상기 재배지(또는 상기 식물)에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계는, 2종 이상의 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 순차적으로 또는 동시에 적용하여 수행되는 것일 수 있다.
다른 예로, 상기 식물은 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하도록 유전적으로 조작된 것이고, 상기 재배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제 및 제2의 제초제의 유효량을 순차적으로 또는 동시에 적용하는 것일 수 있다.
다른 예는, 상기 폴리펩타이드, 폴리펩타이드 변이체, 이를 암호화하는 폴리뉴클레오타이드, 상기 폴리뉴클레오타이드를 포함하는 재조합 벡터, 및 상기 재조합 벡터를 포함하는 재조합 세포로 이루어진 군에서 선택된 하나 이상을 포함하는 군에서 선택되는 1종 이상을 포함하는 조류를 배양 배지에 제공하는 단계, 및 상기 배양 배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계를 포함하는, 재배지에서 원하지 않는 생물들을 제거하는 방법을 제공한다.
식물 또는 조류에 제초제에 대한 내성을 부여 및/또는 증진하는 기술이 제공된다.
본 명세서에서, '식물 또는 조류의 제초제에 대한 내성 부여 및/또는 증진' 또는 '식물 또는 조류의 제초제에 대한 내성 증진' 이란 제초제에 내성이 없는 식물 또는 조류에 대하여 내성을 부여하는 것, 또는 제초제에 내성을 지닌 식물 또는 조류에 대하여 그 내성을 보다 향상시키는 것, 또는 이 모두를 포괄하는 광범위한 의미로 해석된다.
본 명세서에서, '소정의 서열로 이루어진', '소정의 서열을 필수적으로 하여 이루어진' 또는 '소정의 서열을 포함하는' 이라 함은 기재된 서열을 포함하거나, 상기 서열을 필수적으로 포함하는 경우를 모두 의미하기 위하여 사용되며, 기재된 서열 이외의 서열을 포함하는 것을 배제하고자 하는 의도로 해석되지 않는다.
본 명세서에서, '서열번호로 기재된 아미노산 서열을 포함하거나 상기 서열로 이루어진 단백질 또는 폴리펩타이드' 및 '서열번호로 기재된 핵산 서열을 포함하거나 상기 서열로 이루어진 유전자 또는 폴리뉴클레오타이드'는 해당 서열번호의 서열을 필수적으로 포함하거나, 상기 단백질 (또는 폴리펩타이드) 또는 유전자 (또는 폴리뉴클레오타이드)의 본래의 활성 및/또는 기능을 유지하면서 해당 서열번호의 서열과 90% 이상, 91% 이상, 92% 이상, 93% 이상, 94% 이상, 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 서열을 포함하는 단백질 (또는 폴리펩타이드) 또는 유전자 (또는 폴리뉴클레오타이드)를 의미하는 것으로 해석될 수 있다.
일 예는 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열을 포함하는 폴리펩타이드를 제공한다.
다른 예에서,
PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 1의 아미노산들 (예컨대, 서열번호 1의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 1의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체;
PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 2의 아미노산들 (예컨대, 서열번호 2의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 2의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체로 이루어진 군에서 선택되는 1종 이상의 폴리펩타이드 변이체; 및
서열번호 3의 폴리펩타이드와 PPO 활성 저해 제초제와의 상호작용에 관여하는 서열번호 3의 아미노산들 (예컨대, 서열번호 3의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 원래의 아미노산과 다른 아미노산으로 치환된, 변이된 서열번호 3의 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드 변이체
로 이루어진 군에서 선택되는 1종 이상의 폴리펩타이드 변이체가 제공된다.
다른 예에서, 서열번호 1, 2, 또는 3의 폴리펩타이드 또는 상기 폴리펩타이드 변이체를 암호화하는 폴리뉴클레오타이드, 상기 폴리뉴클레오타이드를 포함하는 재조합 벡터, 및 상기 재조합 벡터를 포함하는 재조합 세포가 제공된다. 상기 폴리뉴클레오타이드는 각 아미노산을 암호화하는 코돈들 중에서 형질도입 될 세포에 최적화된 (optimized) 코돈을 포함하도록 설계된 것일 수 있다. 상기 최적화 코돈은 이 발명이 속하는 기술 분야의 통상의 지식을 가진 자라면 용이하게 알 수 있다 (예컨대, "http://sg.idtdna.com/CodonOpt" 등 참조).
다른 예에서,
(1) 서열번호 1의 폴리펩타이드, 서열번호 1의 폴리펩타이드의 변이체, 서열번호 2의 폴리펩타이드, 서열번호 2의 폴리펩타이드의 변이체, 서열번호 3의 폴리펩타이드, 서열번호 3의 폴리펩타이드의 변이체, 및 상기 폴리펩타이드 또는 폴리펩타이드 변이체와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드 또는 폴리펩타이드 변이체;
(2) 상기 (1)의 폴리펩타이드 또는 폴리펩타이드 변이체를 암호화하는 폴리뉴클레오타이드;
(3) 상기 (2)의 폴리뉴클레오타이드를 포함하는 재조합 벡터; 및
(4) 상기 (3)의 재조합 벡터를 포함하는 재조합 세포
로 이루어진 군에서 선택된 하나 이상을 포함하는, 식물 및/또는 조류의 제초제에 대한 내성 부여 및/또는 증진용 조성물을 제공한다.
일 예에서 상기 서열번호 1의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 80의 핵산 서열을 포함하는 것일 수 있고, 서열번호 2의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 81의 핵산 서열을 포함하는 것일 수 있으며, 서열번호 3의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드는 서열번호 82의 핵산 서열을 포함하는 것일 수 있으나, 이에 제한되는 것은 아니고, codon degeneracy에 따라 상기 아미노산 서열을 암호화하는 다양한 핵산 서열을 포함할 수 있다.
다른 예에서, 상기 폴리펩타이드 또는 폴리펩타이드의 변이체를 암호화하는 폴리뉴클레오타이드로 형질전환된, 제초제에 대한 내성을 가진 형질전환체가 제공된다. 상기 폴리뉴클레오타이드는 각 아미노산을 암호화하는 코돈들 중에서 형질도입될 세포에 최적화된 (optimized) 코돈을 포함하도록 설계된 것일 수 있다. 상기 최적화 코돈은 이 발명이 속하는 기술 분야의 통상의 지식을 가진 자라면 용이하게 알 수 있다 (예컨대, "http://sg.idtdna.com/CodonOpt" 등 참조).
다른 예에서, 상기 폴리뉴클레오타이드를 조류 또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초, 또는 식물전체에 형질전환하는 단계를 포함하는, 제초제에 대한 내성을 가지는 식물 또는 조류의 제조 방법이 제공된다.
다른 예에서, 상기 폴리뉴클레오타이드를 조류 또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초 또는 식물 전체에 형질전환하는 단계를 포함하는, 식물 또는 조류의 제초제에 대한 내성을 부여 및/또는 증진시키는 방법을 제공한다.
이하, 본 발명을 보다 상세하게 설명한다.
본 명세서에서 제공되는 서열번호 1, 2, 및 3의 아미노산 서열을 포함하는 폴리펩타이드는 PPO 저해 제초제에 대하여 내성을 갖는 남세균 유래 PPO 단백질이다.
구체적으로, 써모시네코코커스 에롱가투스 (Thermosynechococcus elongatus) PKUAC-SCTE542에서 유래한 PPO 단백질 (GenBank Accession No. CP032152.1를 포함)이 제공되며, 이를 CyPPO19로 명명하고, 이의 아미노산 서열을 서열번호 1로, 이를 암호화하는 유전자의 염기서열을 서열번호 80으로 각각 나타낸다.
또한, 시아노박테리아 박테리움 (Cyanobacteria bacterium) J003 균주에서 유래한 PPO 단백질 (GenBank Accession No. RMH63851.1)이 제공되며, 이를 CyPPO20으로 명명하고, 이의 아미노산 서열을 서열번호 2로, 이를 암호화하는 유전자의 염기서열을 서열번호 81로 각각 나타낸다.
또한, 써모시네코코커스 불카누스 (Thermosynechococcus vulcanus) NIES-2134에서 유래한 PPO 단백질 (GenBank Accession No. BAY51976.1)이 제공되며, 이를 CyPPO18로 명명하고, 이의 아미노산 서열을 서열번호 3으로, 이를 암호화하는 유전자의 염기서열을 서열번호 82로 각각 나타낸다.
본 명세서에서, 앞서 설명한 폴리펩타이드 및 폴리펩타이드 변이체는 각각 PPO 계열 제초제에 대하여 내성을 갖는 제초제 내성 PPO 단백질 또는 제초제 내성 PPO 단백질 변이체로도 표현될 수 있다. 또한, 본 명세서에 사용된 바로서, "제초제 내성 PPO 단백질 또는 이의 변이체"는 상기한 제초제 내성 PPO 단백질 또는 제초제 내성 PPO 단백질 변이체, 제초제 내성 PPO 단백질 암호화 유전자 또는 제초제 내성 PPO 단백질 변이체 암호화 유전자, 또는 이들의 조합을 의미하기 위하여 사용될 수 있다.
남세균 유래 PPO 단백질은 그 자체로 식물 PPO 보다 효소 활성이 우수한 특성을 가지며 PPO 활성 저해 제초제에 대하여 내성을 부여할 수 있을 뿐 아니라, 이들 PPO 단백질은 고유의 효소 활성을 전체적으로 유지시키는 범위 내에서 아미노산 변이를 포함함으로써 야생형 PPO 단백질보다 제초제 내성을 강화시킬 수 있다. 이러한 아미노산 변이는 PPO 단백질과 제초제 간 상호작용 부위의 아미노산 잔기 중에서 선택된 하나 이상의 아미노산의 치환, 결실, 부가 및/또는 삽입을 포함하는 것일 수 있다.
상기 PPO 단백질의 변이체를 보다 구체적으로 설명하면 다음과 같다.
일 예는 서열번호 1의 폴리펩타이드 (CyPPO19)와 PPO 활성 저해 제초제와의 상호작용과 관여하는 서열번호 1의 아미노산들 (서열번호 1의 폴리펩타이드 (CyPPO19)의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 및/또는 해당 위치의 원래의 아미노산 (즉, 야생형의 해당 위치의 아미노산)과 다른 아미노산으로 치환된 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하거나 상기 아미노산 서열로 이루어진 폴리펩타이드 변이체를 제공한다.
다른 예는 서열번호 2의 폴리펩타이드 (CyPPO20)와 PPO 활성 저해 제초제와의 상호작용과 관여하는 서열번호 2의 아미노산들 (서열번호 2의 폴리펩타이드 (CyPPO20)의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 또는 해당 위치의 원래의 아미노산 (즉, 야생형의 해당 위치의 아미노산)과 다른 아미노산으로 치환된 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하거나 상기 아미노산 서열로 이루어진 폴리펩타이드 변이체를 제공한다.
다른 예는 서열번호 3의 폴리펩타이드 (CyPPO18)와 PPO 활성 저해 제초제와의 상호작용과 관여하는 서열번호 3의 아미노산들 (서열번호 3의 폴리펩타이드 (CyPPO18)의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들)로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 또는 해당 위치의 원래의 아미노산 (즉, 야생형의 해당 위치의 아미노산)과 다른 아미노산으로 치환된 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하거나 상기 아미노산 서열로 이루어진 폴리펩타이드 변이체를 제공한다.
상기 서열번호 1, 서열번호 2, 또는 서열번호 3의 폴리펩타이드의 결실 또는 해당 위치의 원래의 아미노산과 다른 아미노산으로 치환되는 아미노산 잔기 (예컨대, 서열번호 1, 서열번호 2, 또는 서열번호 3의 폴리펩타이드의 PPO 활성 저해 제초제와의 결합 부위에 위치하는 아미노산들로 이루어진 군에서 선택된 하나 이상)는 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열 중의 N59 ("59번째 위치의 N(Asn)"을 의미; 이하의 아미노산 잔기의 표현은 이와 동일한 방식으로 해석됨), S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상, 예컨대, R89, V165, A167, V305, L327, F360, 및 I408로 이루어진 군에서 선택된 하나 이상, 2 이상, 3 이상, 4 이상, 5 이상, 6 이상, 또는 이들 모두일 수 있다.
일 구체예에서, 상기 폴리펩타이드의 변이체는 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열 중의 N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상 (예컨대, R89, V165, A167, V305, L327, F360, 및 I408으로 이루어진 군에서 선택된 하나 이상, 2 이상, 3 이상, 4 이상, 5 이상, 6 이상, 또는 이들 모두)이, 각각 독립적으로, 결실 또는 M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), F(Phe), P(Pro), W(Trp), N(Asn), Q(Gln), G(Gly), Y(Tyr), D(Asp), E(Glu), R(Arg), H(His), K(Lys) 등으로 이루어진 군에서 선택되고 해당 위치의 원래 아미노산과 상이한 아미노산으로 치환 (예컨대, M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), R(Arg), W(Trp) 등으로 이루어진 군에서 선택되고 해당 위치의 원래 아미노산과 상이한 아미노산으로 치환)된 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 것일 수 있다.
예컨대, 상기 폴리펩타이드의 변이체는,
(a) 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열에 있어서,
(i) F360M (360번째 위치의 아미노산 잔기가 F(Phe)에서 M(Met)으로 치환됨을 의미함; 이하의 아미노산 변이의 표현은 이와 동일한 방식으로 해석됨), F360V, F360I, F360T, 또는 F360L,
(ii) A167C, A167L, 또는 A167I,
(iii) V305M 또는 V305L,
(iv) R89A,
(v) V165S 또는 V165C,
(vi) L327T, 및
(vii) I408R 또는 I408W
로 이루어진 군에서 선택된 하나 이상, 2 이상, 3 이상, 4 이상, 5 이상, 6 이상, 또는 모든 아미노산 변이를 포함하는 아미노산 서열, 또는
(b) 상기 (a)의 아미노산 서열과 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열
을 포함하는 것일 수 있다.
보다 구체적으로, 상기 폴리펩타이드의 변이체는 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열에 있어서, 다음의 아미노산 변이를 포함하는 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 것일 수 있다: R89A, V165C, V165S, A167C, A167I, A167L, V305L, V305M, L327T, F360M, F360I, F360L, F360V, F360T, I408R, I408W, R89A+V165C(89번째 잔기의 R에서 A로의 치환 및 165번째 잔기의 V에서 C로의 치환을 모두 포함; 이하의 2가지 이상의 아미노산 변이의 표현은 이와 동일한 방식으로 해석됨), R89A+V165S, R89A+A167C, R89A+A167I, R89A+A167L, R89A+V305L, R89A+V305M, R89A+L327T, R89A+F360M, R89A+F360I, R89A+F360L, R89A+F360V, R89A+F360T, R89A+I408R, V165C+A167C, V165C+A167I, V165C+A167L, V165C+V305L, V165C+V305M, V165C+L327T, V165C+F360M, V165C+F360I, V165C+F360L, V165C+F360V, V165C+F360T, V165C+I408R, V165S+A167C, V165S+A167I, V165S+A167L, V165S+V305L, V165S+V305M, V165S+L327T, V165S+F360M, V165S+F360I, V165S+F360L, V165S+F360V, V165S+F360T, V165S+I408W, A167C+V305L, A167C+V305M, A167C+L327T, A167C+F360M, A167C+F360I, A167C+F360L, A167C+F360V, A167C+F360T, A167C+I408R, A167I+V305L, A167I+V305M, A167I+L327T, A167I+F360M, A167I+F360I, A167I+F360L, A167I+F360V, A167I+F360T, A167I+I408W, A167L+V305L, A167L+V305M, A167L+L327T, A167L+F360M, A167L+F360I, A167L+F360L, A167L+F360V, A167L+F360T, A167L+I408R, V305L+L327T, V305L+F360M, V305L+F360I, V305L+F360L, V305L+F360V, V305L+F360T, V305L+I408W, V305M+L327T, V305M+F360M, V305M+F360I, V305M+F360L, V305M+F360V, V305M+F360T, V305M+I408R, L327T+F360M, L327T+F360I, L327T+F360L, L327T+F360V, L327T+F360T, L327T+I408R, F360M+I408R, F360I+I408R, F360L+I408W, F360V+I408W, F360T+I408W, R89A+V165C+A167C, R89A+V165C+A167I, R89A+V165C+A167L, R89A+V165C+V305L, R89A+V165C+V305M, R89A+V165C+L327T, R89A+V165C+F360M, R89A+V165C+F360I, R89A+V165C+F360L, R89A+V165C+F360V, R89A+V165C+F360T, R89A+V165C+I408R, R89A+V165S+A167C, R89A+V165S+A167I, R89A+V165S+A167L, R89A+V165S+V305L, R89A+V165S+V305M, R89A+V165S+L327T, R89A+V165S+F360M, R89A+V165S+F360I, R89A+V165S+F360L, R89A+V165S+F360V, R89A+V165S+F360T, R89A+V165S+I408W, R89A+A167C+V305L, R89A+A167C+V305M, R89A+A167C+L327T, R89A+A167C+F360M, R89A+A167C+F360I, R89A+A167C+F360L, R89A+A167C+F360V, R89A+A167C+F360T, R89A+A167C+I408R, R89A+A167I+V305L, R89A+A167I+V305M, R89A+A167I+L327T, R89A+A167I+F360M, R89A+A167I+F360I, R89A+A167I+F360L, R89A+A167I+F360V, R89A+A167I+F360T, R89A+A167I+I408W, R89A+A167L+V305L, R89A+A167L+V305M, R89A+A167L+L327T, R89A+A167L+F360M, R89A+A167L+F360I, R89A+A167L+F360L, R89A+A167L+F360V, R89A+A167L+F360T, R89A+A167L+I408R, R89A+V305L+L327T, R89A+V305L+F360M, R89A+V305L+F360I, R89A+V305L+F360L, R89A+V305L+F360V, R89A+V305L+F360T, R89A+V305L+I408W, R89A+V305M+L327T, R89A+V305M+F360M, R89A+V305M+F360I, R89A+V305M+F360L, R89A+V305M+F360V, R89A+V305M+F360T, R89A+V305M+I408R, R89A+L327T+F360M, R89A+L327T+F360I, R89A+L327T+F360L, R89A+L327T+F360V, R89A+L327T+F360T, R89A+L327T+I408R, R89A+F360M+I408R, R89A+F360I+I408R, R89A+F360L+I408W, R89A+F360V+I408W, R89A+F360T+I408W, V165C+A167C+V305L, V165C+A167C+V305M, V165C+A167C+L327T, V165C+A167C+F360M, V165C+A167C+F360I, V165C+A167C+F360L, V165C+A167C+F360V, V165C+A167C+F360T, V165C+A167C+I408R, V165C+A167I+V305L, V165C+A167I+V305M, V165C+A167I+L327T, V165C+A167I+F360M, V165C+A167I+F360I, V165C+A167I+F360L, V165C+A167I+F360V, V165C+A167I+F360T, V165C+A167I+I408W, V165C+A167L+V305L, V165C+A167L+V305M, V165C+A167L+L327T, V165C+A167L+F360M, V165C+A167L+F360I, V165C+A167L+F360L, V165C+A167L+F360V, V165C+A167L+F360T, V165C+A167L+I408R, V165C+V305L+L327T, V165C+V305L+F360M, V165C+V305L+F360I, V165C+V305L+F360L, V165C+V305L+F360V, V165C+V305L+F360T, V165C+V305L+F360T, V165C+V305L+I408W, V165C+V305M+L327T, V165C+V305M+F360M, V165C+V305M+F360I, V165C+V305M+F360L, V165C+V305M+F360V, V165C+V305M+F360T, V165C+V305M+I408R, V165C+L327T+F360M, V165C+L327T+F360I, V165C+L327T+F360L, V165C+L327T+F360V, V165C+L327T+F360T, V165C+L327T+I408R, V165C+F360M+I408R, V165C+F360I+I408R, V165C+F360L+I408W, V165C+F360V+I408W, V165C+F360T+I408W, V165S+A167C+V305L, V165S+A167C+V305M, V165S+A167C+L327T, V165S+A167C+F360M, V165S+A167C+F360I, V165S+A167C+F360L, V165S+A167C+F360V, V165S+A167C+F360T, V165S+A167C+I408R, V165S+A167I+V305L, V165S+A167I+V305M, V165S+A167I+L327T, V165S+A167I+F360M, V165S+A167I+F360I, V165S+A167I+F360L, V165S+A167I+F360V, V165S+A167I+F360T, V165S+A167I+I408W, V165S+A167L+V305L, V165S+A167L+V305M, V165S+A167L+L327T, V165S+A167L+F360M, V165S+A167L+F360I, V165S+A167L+F360L, V165S+A167L+F360V, V165S+A167L+F360T, V165S+A167L+I408R, V165S+V305L+L327T, V165S+V305L+F360M, V165S+V305L+F360I, V165S+V305L+F360L, V165S+V305L+F360V, V165S+V305L+F360T, V165S+V305L+I408W, V165S+V305M+L327T, V165S+V305M+F360M, V165S+V305M+F360I, V165S+V305M+F360L, V165S+V305M+F360V, V165S+V305M+F360T, V165S+V305M+I408R, V165S+L327T+F360M, V165S+L327T+F360I, V165S+L327T+F360L, V165S+L327T+F360V, V165S+L327T+F360T, V165S+L327T+I408R, V165S+F360M+I408R, V165S+F360I+I408R, V165S+F360L+I408W, V165S+F360V+I408W, V165S+F360T+I408W, A167C+V305L+L327T, A167C+V305L+F360M, A167C+V305L+F360I, A167C+V305L+F360L, A167C+V305L+F360V, A167C+V305L+F360T, A167C+V305L+I408W, A167C+V305M+L327T, A167C+V305M+F360M, A167C+V305M+F360I, A167C+V305M+F360L, A167C+V305M+F360V, A167C+V305M+F360T, A167C+V305M+I408R, A167C+L327T+F360M, A167C+L327T+F360I, A167C+L327T+F360L, A167C+L327T+F360V, A167C+L327T+F360T, A167C+L327T+I408R, A167C+F360M+I408R, A167C+F360I+I408R, A167C+F360L+I408W, A167C+F360V+I408W, A167C+F360T+I408W, A167I+V305L+L327T, A167I+V305L+F360M, A167I+V305L+F360I, A167I+V305L+F360L, A167I+V305L+F360V, A167I+V305L+F360T, A167I+V305L+I408W, A167I+V305M+L327T, A167I+V305M+F360M, A167I+V305M+F360I, A167I+V305M+F360L, A167I+V305M+F360V, A167I+V305M+F360T, A167I+V305M+I408R, A167I+L327T+F360M, A167I+L327T+F360I, A167I+L327T+F360L, A167I+L327T+F360V, A167I+L327T+F360T, A167I+L327T+I408R, A167I+F360M+I408R, A167I+F360I+I408R, A167I+F360L+I408W, A167I+F360V+I408W, A167I+F360T+I408W, A167L+V305L+L327T, A167L+V305L+F360M, A167L+V305L+F360I, A167L+V305L+F360L, A167L+V305L+F360V, A167L+V305L+F360T, A167L+V305L+I408W, A167L+V305M+L327T, A167L+V305M+F360M, A167L+V305M+F360I, A167L+V305M+F360L, A167L+V305M+F360V, A167L+V305M+F360T, A167L+V305M+I408R, A167L+L327T+F360M, A167L+L327T+F360I, A167L+L327T+F360L, A167L+L327T+F360V, A167L+L327T+F360T, A167L+L327T+I408R, A167L+F360M+I408R, A167L+F360I+I408R, A167L+F360L+I408W, A167L+F360V+I408W, A167L+F360T+I408W, V305L+L327T+F360M, V305L+L327T+F360I, V305L+L327T+F360L, V305L+L327T+F360V, V305L+L327T+F360T, V305L+L327T+I408R, V305L+F360M+I408R, V305L+F360I+I408R, V305L+F360L+I408W, V305L+F360V+I408W, V305L+F360T+I408W, V305M+L327T+F360M, V305M+L327T+F360I, V305M+L327T+F360L, V305M+L327T+F360V, V305M+L327T+F360T, V305M+L327T+I408R, V305M+F360M+I408R, V305M+F360I+I408R, V305M+F360L+I408W, V305M+F360V+I408W, V305M+F360T+I408W, L327T+F360M+I408R, L327T+F360I+I408R, L327T+F360T+I408R, L327T+F360L+I408W, L327T+F360V+I408W, L327T+F360T+I408W, R89A+V165C+A167C+V305L, R89A+V165C+A167C+V305M, R89A+V165C+A167C+L327T, R89A+V165C+A167C+F360M, R89A+V165C+A167C+F360I, R89A+V165C+A167C+F360L, R89A+V165C+A167C+F360V, R89A+V165C+A167C+F360T, R89A+V165C+A167C+I408R, R89A+V165C+A167I+V305L, R89A+V165C+A167I+V305M, R89A+V165C+A167I+L327T, R89A+V165C+A167I+F360M, R89A+V165C+A167I+F360I, R89A+V165C+A167I+F360L, R89A+V165C+A167I+F360V, R89A+V165C+A167I+I408W, R89A+V165C+A167L+V305L, R89A+V165C+A167L+V305M, R89A+V165C+A167L+L327T, R89A+V165C+A167L+F360M, R89A+V165C+A167L+F360I, R89A+V165C+A167L+F360L, R89A+V165C+A167L+F360V, R89A+V165C+A167L+I408R, R89A+V165C+V305L+L327T, R89A+V165C+V305L+F360M, R89A+V165C+V305L+F360I, R89A+V165C+V305L+F360L, R89A+V165C+V305L+F360V, R89A+V165C+V305L+I408W, R89A+V165C+V305M+L327T, R89A+V165C+V305M+F360M, R89A+V165C+V305M+F360I, R89A+V165C+V305M+F360L, R89A+V165C+V305M+F360V, R89A+V165C+V305M+I408R, R89A+V165C+L327T+F360M, R89A+V165C+L327T+F360I, R89A+V165C+L327T+F360L, R89A+V165C+L327T+F360V, R89A+V165C+L327T+I408R, R89A+V165C+F360M+I408R, R89A+V165C+F360I+I408R, R89A+V165C+F360L+I408W, R89A+V165C+F360V+I408W, R89A+V165S+A167C+V305L, R89A+V165S+A167C+V305M, R89A+V165S+A167C+L327T, R89A+V165S+A167C+F360M, R89A+V165S+A167C+F360I, R89A+V165S+A167C+F360L, R89A+V165S+A167C+F360V, R89A+V165S+A167C+I408R, R89A+V165S+A167I+V305L, R89A+V165S+A167I+V305M, R89A+V165S+A167I+L327T, R89A+V165S+A167I+F360M, R89A+V165S+A167I+F360I, R89A+V165S+A167I+F360L, R89A+V165S+A167I+F360V, R89A+V165S+A167I+I408W, R89A+V165S+A167L+V305L, R89A+V165S+A167L+V305M, R89A+V165S+A167L+L327T, R89A+V165S+A167L+F360M, R89A+V165S+A167L+F360I, R89A+V165S+A167L+F360L, R89A+V165S+A167L+F360V, R89A+V165S+A167L+I408R, R89A+V165S+V305L+L327T, R89A+V165S+V305L+F360M, R89A+V165S+V305L+F360I, R89A+V165S+V305L+F360L, R89A+V165S+V305L+F360V, R89A+V165S+V305L+I408W, R89A+V165S+V305M+L327T, R89A+V165S+V305M+F360M, R89A+V165S+V305M+F360I, R89A+V165S+V305M+F360L, R89A+V165S+V305M+F360V, R89A+V165S+V305M+I408R, R89A+V165S+L327T+F360M, R89A+V165S+L327T+F360I, R89A+V165S+L327T+F360L, 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R89A+V165C+A167I+V305L+F360L+I408W, R89A+V165C+A167I+V305L+F360V+I408W, R89A+V165C+A167I+V305M+L327T+F360M, R89A+V165C+A167I+V305M+L327T+F360I, R89A+V165C+A167I+V305M+L327T+F360L, R89A+V165C+A167I+V305M+L327T+F360V, R89A+V165C+A167I+V305M+L327T+I408R, R89A+V165C+A167I+V305M+F360M+I408R, R89A+V165C+A167I+V305M+F360I+I408R, R89A+V165C+A167I+V305M+F360L+I408W, R89A+V165C+A167I+V305M+F360V+I408W, R89A+V165C+A167I+L327T+F360M+I408R, R89A+V165C+A167I+L327T+F360I+I408R, R89A+V165C+A167I+L327T+F360L+I408W, R89A+V165C+A167I+L327T+F360V+I408W, R89A+V165C+A167L+V305L+L327T+F360M, R89A+V165C+A167L+V305L+L327T+F360I, R89A+V165C+A167L+V305L+L327T+F360L, R89A+V165C+A167L+V305L+L327T+F360V, R89A+V165C+A167L+V305L+L327T+I408R, R89A+V165C+A167L+V305L+F360M+I408R, R89A+V165C+A167L+V305L+F360I+I408R, R89A+V165C+A167L+V305L+F360L+I408W, R89A+V165C+A167L+V305L+F360V+I408W, R89A+V165C+A167L+V305M+L327T+F360M, R89A+V165C+A167L+V305M+L327T+F360I, R89A+V165C+A167L+V305M+L327T+F360L, R89A+V165C+A167L+V305M+L327T+F360V, R89A+V165C+A167L+V305M+L327T+I408R, R89A+V165C+A167L+V305M+F360M+I408R, R89A+V165C+A167L+V305M+F360I+I408R, R89A+V165C+A167L+V305M+F360L+I408W, R89A+V165C+A167L+V305M+F360V+I408W, R89A+V165C+A167L+L327T+F360M+I408R, R89A+V165C+A167L+L327T+F360I+I408R, R89A+V165C+A167L+L327T+F360L+I408W, R89A+V165C+A167L+L327T+F360V+I408W, R89A+V165C+V305L+L327T+F360M+I408R, R89A+V165C+V305L+L327T+F360I+I408R, R89A+V165C+V305L+L327T+F360L+I408W, R89A+V165C+V305L+L327T+F360V+I408W, R89A+V165C+V305M+L327T+F360M+I408R, R89A+V165C+V305M+L327T+F360I+I408R, R89A+V165C+V305M+L327T+F360L+I408W, R89A+V165C+V305M+L327T+F360V+I408W, R89A+V165S+A167C+V305L+L327T+F360M, R89A+V165S+A167C+V305L+L327T+F360I, R89A+V165S+A167C+V305L+L327T+F360L, R89A+V165S+A167C+V305L+L327T+F360V, R89A+V165S+A167C+V305L+L327T+I408R, R89A+V165S+A167C+V305L+F360M+I408R, R89A+V165S+A167C+V305L+F360I+I408R, R89A+V165S+A167C+V305L+F360L+I408W, R89A+V165S+A167C+V305L+F360V+I408W, R89A+V165S+A167C+V305M+L327T+F360M, R89A+V165S+A167C+V305M+L327T+F360I, R89A+V165S+A167C+V305M+L327T+F360L, R89A+V165S+A167C+V305M+L327T+F360V, R89A+V165S+A167C+V305M+L327T+I408R, R89A+V165S+A167C+V305M+F360M+I408R, R89A+V165S+A167C+V305M+F360I+I408R, R89A+V165S+A167C+V305M+F360L+I408W, R89A+V165S+A167C+V305M+F360V+I408W, R89A+V165S+A167C+L327T+F360M+I408R, R89A+V165S+A167C+L327T+F360I+I408R, R89A+V165S+A167C+L327T+F360L+I408W, R89A+V165S+A167C+L327T+F360V+I408W, R89A+V165S+A167I+V305L+L327T+F360M, R89A+V165S+A167I+V305L+L327T+F360I, R89A+V165S+A167I+V305L+L327T+F360L, R89A+V165S+A167I+V305L+L327T+F360V, R89A+V165S+A167I+V305L+L327T+I408R, R89A+V165S+A167I+V305L+F360M+I408R, R89A+V165S+A167I+V305L+F360I+I408R, R89A+V165S+A167I+V305L+F360L+I408W, R89A+V165S+A167I+V305L+F360V+I408W, R89A+V165S+A167I+V305M+L327T+F360M, R89A+V165S+A167I+V305M+L327T+F360I, R89A+V165S+A167I+V305M+L327T+F360L, R89A+V165S+A167I+V305M+L327T+F360V, R89A+V165S+A167I+V305M+L327T+I408R, R89A+V165S+A167I+V305M+F360M+I408R, R89A+V165S+A167I+V305M+F360I+I408R, R89A+V165S+A167I+V305M+F360L+I408W, R89A+V165S+A167I+V305M+F360V+I408W, R89A+V165S+A167I+L327T+F360M+I408R, R89A+V165S+A167I+L327T+F360I+I408R, R89A+V165S+A167I+L327T+F360L+I408W, R89A+V165S+A167I+L327T+F360V+I408W, R89A+V165S+A167L+V305L+L327T+F360M, R89A+V165S+A167L+V305L+L327T+F360I, R89A+V165S+A167L+V305L+L327T+F360L, R89A+V165S+A167L+V305L+L327T+F360V, R89A+V165S+A167L+V305L+L327T+I408R, R89A+V165S+A167L+V305L+F360M+I408R, R89A+V165S+A167L+V305L+F360I+I408R, R89A+V165S+A167L+V305L+F360L+I408W, R89A+V165S+A167L+V305L+F360V+I408W, R89A+V165S+A167L+V305M+L327T+F360M, R89A+V165S+A167L+V305M+L327T+F360I, R89A+V165S+A167L+V305M+L327T+F360L, R89A+V165S+A167L+V305M+L327T+F360V, R89A+V165S+A167L+V305M+L327T+I408R, R89A+V165S+A167L+V305M+F360M+I408R, R89A+V165S+A167L+V305M+F360I+I408R, R89A+V165S+A167L+V305M+F360L+I408W, R89A+V165S+A167L+V305M+F360V+I408W, R89A+V165S+A167L+L327T+F360M+I408R, R89A+V165S+A167L+L327T+F360I+I408R, R89A+V165S+A167L+L327T+F360L+I408W, R89A+V165S+A167L+L327T+F360V+I408W, R89A+V165S+V305L+L327T+F360M+I408R, R89A+V165S+V305L+L327T+F360I+I408R, R89A+V165S+V305L+L327T+F360L+I408W, R89A+V165S+V305L+L327T+F360V+I408W, R89A+V165S+V305M+L327T+F360M+I408R, R89A+V165S+V305M+L327T+F360I+I408R, R89A+V165S+V305M+L327T+F360L+I408W, R89A+V165S+V305M+L327T+F360V+I408W, R89A+A167C+V305L+L327T+F360M+I408R, R89A+A167C+V305L+L327T+F360I+I408R, R89A+A167C+V305L+L327T+F360L+I408W, R89A+A167C+V305L+L327T+F360V+I408W, R89A+A167C+V305M+L327T+F360M+I408R, R89A+A167C+V305M+L327T+F360I+I408R, R89A+A167C+V305M+L327T+F360L+I408W, R89A+A167C+V305M+L327T+F360V+I408W, R89A+A167I+V305L+L327T+F360M+I408R, R89A+A167I+V305L+L327T+F360I+I408R, R89A+A167I+V305L+L327T+F360L+I408W, R89A+A167I+V305L+L327T+F360V+I408W, R89A+A167I+V305M+L327T+F360M+I408R, R89A+A167I+V305M+L327T+F360I+I408R, R89A+A167I+V305M+L327T+F360L+I408W, R89A+A167I+V305M+L327T+F360V+I408W, R89A+A167L+V305L+L327T+F360M+I408R, R89A+A167L+V305L+L327T+F360I+I408R, R89A+A167L+V305L+L327T+F360L+I408W, R89A+A167L+V305L+L327T+F360V+I408W, R89A+A167L+V305M+L327T+F360M+I408R, R89A+A167L+V305M+L327T+F360I+I408R, R89A+A167L+V305M+L327T+F360L+I408W, R89A+A167L+V305M+L327T+F360V+I408W, V165C+A167C+V305L+L327T+F360M+I408R, V165C+A167C+V305L+L327T+F360I+I408R, V165C+A167C+V305L+L327T+F360L+I408W, V165C+A167C+V305L+L327T+F360V+I408W, V165C+A167C+V305M+L327T+F360M+I408R, V165C+A167C+V305M+L327T+F360I+I408R, V165C+A167C+V305M+L327T+F360L+I408W, V165C+A167C+V305M+L327T+F360V+I408W, V165C+A167I+V305L+L327T+F360M+I408R, V165C+A167I+V305L+L327T+F360I+I408R, V165C+A167I+V305L+L327T+F360L+I408W, V165C+A167I+V305L+L327T+F360V+I408W, V165C+A167I+V305M+L327T+F360M+I408R, V165C+A167I+V305M+L327T+F360I+I408R, V165C+A167I+V305M+L327T+F360L+I408W, V165C+A167I+V305M+L327T+F360V+I408W, V165C+A167L+V305L+L327T+F360M+I408R, V165C+A167L+V305L+L327T+F360I+I408R, V165C+A167L+V305L+L327T+F360L+I408W, V165C+A167L+V305L+L327T+F360V+I408W, V165C+A167L+V305M+L327T+F360M+I408R, V165C+A167L+V305M+L327T+F360I+I408R, V165C+A167L+V305M+L327T+F360L+I408W, V165C+A167L+V305M+L327T+F360V+I408W, V165S+A167C+V305L+L327T+F360M+I408R, V165S+A167C+V305L+L327T+F360I+I408R, V165S+A167C+V305L+L327T+F360L+I408W, V165S+A167C+V305L+L327T+F360V+I408W, V165S+A167C+V305M+L327T+F360M+I408R, V165S+A167C+V305M+L327T+F360I+I408R, V165S+A167C+V305M+L327T+F360L+I408W, V165S+A167C+V305M+L327T+F360V+I408W, V165S+A167I+V305L+L327T+F360M+I408R, V165S+A167I+V305L+L327T+F360I+I408R, V165S+A167I+V305L+L327T+F360L+I408W, V165S+A167I+V305L+L327T+F360V+I408W, V165S+A167I+V305M+L327T+F360M+I408R, V165S+A167I+V305M+L327T+F360I+I408R, V165S+A167I+V305M+L327T+F360L+I408W, V165S+A167I+V305M+L327T+F360V+I408W, V165S+A167L+V305L+L327T+F360M+I408R, V165S+A167L+V305L+L327T+F360I+I408R, V165S+A167L+V305L+L327T+F360L+I408W, V165S+A167L+V305L+L327T+F360V+I408W, V165S+A167L+V305M+L327T+F360M+I408R, V165S+A167L+V305M+L327T+F360I+I408R, V165S+A167L+V305M+L327T+F360L+I408W, V165S+A167L+V305M+L327T+F360V+I408W, R89A+V165C+A167C+V305L+L327T+F360T, R89A+V165C+A167C+V305L+F360T+I408W, R89A+V165C+A167C+V305M+L327T+F360T, R89A+V165C+A167C+V305M+F360T+I408R, R89A+V165C+A167C+L327T+F360T+I408W, R89A+V165C+A167I+V305L+L327T+F360T, R89A+V165C+A167I+V305L+F360T+I408R, R89A+V165C+A167I+V305M+L327T+F360T, R89A+V165C+A167I+V305M+F360T+I408W, R89A+V165C+A167I+L327T+F360T+I408R, R89A+V165C+A167L+V305L+L327T+F360T, R89A+V165C+A167L+V305L+F360T+I408W, R89A+V165C+A167L+V305M+L327T+F360T, R89A+V165C+A167L+V305M+F360T+I408R, R89A+V165C+A167L+L327T+F360T+I408W, R89A+V165C+V305L+L327T+F360T+I408R, R89A+V165C+V305M+L327T+F360T+I408W, R89A+V165S+A167C+V305L+L327T+F360T, R89A+V165S+A167C+V305L+F360T+I408R, R89A+V165S+A167C+V305M+L327T+F360T, R89A+V165S+A167C+V305M+F360T+I408W, R89A+V165S+A167C+L327T+F360T+I408R, R89A+V165S+A167I+V305L+L327T+F360T, R89A+V165S+A167I+V305L+F360T+I408W, R89A+V165S+A167I+V305M+L327T+F360T, R89A+V165S+A167I+V305M+F360T+I408R, R89A+V165S+A167I+L327T+F360T+I408W, R89A+V165S+A167L+V305L+L327T+F360T, R89A+V165S+A167L+V305L+F360T+I408R, R89A+V165S+A167L+V305M+L327T+F360T, R89A+V165S+A167L+V305M+F360T+I408W, R89A+V165S+A167L+L327T+F360T+I408R, R89A+V165S+V305L+L327T+F360T+I408W, R89A+V165S+V305M+L327T+F360T+I408R, R89A+A167C+V305L+L327T+F360T+I408W, R89A+A167C+V305M+L327T+F360T+I408R, R89A+A167I+V305L+L327T+F360T+I408W, R89A+A167I+V305M+L327T+F360T+I408R, R89A+A167L+V305L+L327T+F360T+I408W, R89A+A167L+V305M+L327T+F360T+I408R, V165C+A167C+V305L+L327T+F360T+I408W, V165C+A167C+V305M+L327T+F360T+I408R, V165C+A167I+V305L+L327T+F360T+I408W, V165C+A167I+V305M+L327T+F360T+I408R, V165C+A167L+V305L+L327T+F360T+I408W, V165C+A167L+V305M+L327T+F360T+I408R, V165S+A167C+V305L+L327T+F360T+I408W, V165S+A167C+V305M+L327T+F360T+I408R, V165S+A167I+V305L+L327T+F360T+I408W, V165S+A167I+V305M+L327T+F360T+I408R, V165S+A167L+V305L+L327T+F360T+I408W, V165S+A167L+V305M+L327T+F360T+I408R, R89A+V165C+A167C+V305L+L327T+F360M+I408R, R89A+V165C+A167C+V305L+L327T+F360I+I408R, R89A+V165C+A167C+V305L+L327T+F360L+I408W, R89A+V165C+A167C+V305L+L327T+F360V+I408W, R89A+V165C+A167C+V305M+L327T+F360M+I408R, R89A+V165C+A167C+V305M+L327T+F360I+I408R, R89A+V165C+A167C+V305M+L327T+F360L+I408W, R89A+V165C+A167C+V305M+L327T+F360V+I408W, R89A+V165C+A167I+V305L+L327T+F360M+I408R, R89A+V165C+A167I+V305L+L327T+F360I+I408R, R89A+V165C+A167I+V305L+L327T+F360L+I408W, R89A+V165C+A167I+V305L+L327T+F360V+I408W, R89A+V165C+A167I+V305M+L327T+F360M+I408R, R89A+V165C+A167I+V305M+L327T+F360I+I408R, R89A+V165C+A167I+V305M+L327T+F360L+I408W, R89A+V165C+A167I+V305M+L327T+F360V+I408W, R89A+V165C+A167L+V305L+L327T+F360M+I408R, R89A+V165C+A167L+V305L+L327T+F360I+I408R, R89A+V165C+A167L+V305L+L327T+F360L+I408W, R89A+V165C+A167L+V305L+L327T+F360V+I408W, R89A+V165C+A167L+V305M+L327T+F360M+I408R, R89A+V165C+A167L+V305M+L327T+F360I+I408R, R89A+V165C+A167L+V305M+L327T+F360L+I408W, R89A+V165C+A167L+V305M+L327T+F360V+I408W, R89A+V165S+A167C+V305L+L327T+F360M+I408R, R89A+V165S+A167C+V305L+L327T+F360I+I408R, R89A+V165S+A167C+V305L+L327T+F360L+I408W, R89A+V165S+A167C+V305L+L327T+F360V+I408W, R89A+V165S+A167C+V305M+L327T+F360M+I408R, R89A+V165S+A167C+V305M+L327T+F360I+I408R, R89A+V165S+A167C+V305M+L327T+F360L+I408W, R89A+V165S+A167C+V305M+L327T+F360V+I408W, R89A+V165S+A167I+V305L+L327T+F360M+I408R, R89A+V165S+A167I+V305L+L327T+F360I+I408R, R89A+V165S+A167I+V305L+L327T+F360L+I408W, R89A+V165S+A167I+V305L+L327T+F360V+I408W, R89A+V165S+A167I+V305M+L327T+F360M+I408R, R89A+V165S+A167I+V305M+L327T+F360I+I408R, R89A+V165S+A167I+V305M+L327T+F360L+I408W, R89A+V165S+A167I+V305M+L327T+F360V+I408W, R89A+V165S+A167L+V305L+L327T+F360M+I408R, R89A+V165S+A167L+V305L+L327T+F360I+I408R, R89A+V165S+A167L+V305L+L327T+F360L+I408W, R89A+V165S+A167L+V305L+L327T+F360V+I408W, R89A+V165S+A167L+V305M+L327T+F360M+I408R, R89A+V165S+A167L+V305M+L327T+F360I+I408R, R89A+V165S+A167L+V305M+L327T+F360L+I408W, R89A+V165S+A167L+V305M+L327T+F360V+I408W, R89A+V165C+A167C+V305L+L327T+F360T+I408W, R89A+V165C+A167C+V305M+L327T+F360T+I408R, R89A+V165C+A167I+V305L+L327T+F360T+I408W, R89A+V165C+A167I+V305M+L327T+F360T+I408R, R89A+V165C+A167L+V305L+L327T+F360T+I408W, R89A+V165C+A167L+V305M+L327T+F360T+I408R, R89A+V165S+A167C+V305L+L327T+F360T+I408W, R89A+V165S+A167C+V305M+L327T+F360T+I408R, R89A+V165S+A167I+V305L+L327T+F360T+I408W, R89A+V165S+A167I+V305M+L327T+F360T+I408R, R89A+V165S+A167L+V305L+L327T+F360T+I408W, 또는 R89A+V165S+A167L+V305M+L327T+F360T+I408R.
일 구체예에서, 상기 폴리펩타이드의 변이체는 서열번호 1의 아미노산 서열에 있어서, R89A, V165C, V165S, A167C, A167I, A167L, V305M, V305L, L327T, F360M, F360I, F360L, F360V, F360T, I408R, I408W, R89A+F360M, R89A+F360V, R89A+F360I, R89A+F360L, R89A+F360T, V165C+F360I, V165C+F360M, V165C+F360V, V165C+F360L, V165S+F360V, V165S+F360L, V165S+F360T, A167C+F360I, A167L+F360M, A167I+F360L, A167L+F360T, A167C+F360M, A167C+F360L, A167C+F360V, V305M+F360I, V305L+F360M, V305M+F360M, V305M+F360V, V305M+F360L, V305L+F360L, V305M+F360T, L327T+F360I, L327T+F360M, L327T+F360V, L327T+F360L, L327T+F360T, I408W+F360I, I408R+F360M, I408W+F360V, I408R+F360L, I408W+F360T, R89A+V165C+F360I, R89A+V165S+F360M, R89A+V165C+F360V, R89A+A167I+F360V, R89A+A167C+F360L, R89A+A167L+F360T, R89A+V305M+F360I, R89A+V305M+F360V, R89A+V305M+F360T, R89A+L327T+F360I, R89A+L327T+F360M, R89A+L327T+F360T, R89A+I408R+F360M, R89A+I408W+F360V, R89A+I408R+F360L, V165S+A167I+F360M, V165S+A167C+F360L, V165C+A167L+F360T, V165C+V305M+F360I, V165C+V305M+F360V, V165S+V305L+F360L, V165C+L327T+F360I, V165C+L327T+F360V, V165S+L327T+F360T, V165C+I408W+F360V, V165C+I408W+F360T, A167I+V305M+F360L, A167C+V305L+F360V, A167L+V305M+F360T, A167C+L327T+F360I, A167L+L327T+F360M, A167I+L327T+F360V, A167C+I408W+F360I, A167I+I408W+F360V, A167L+I408R+F360L, V305M+L327T+F360I, V305L+L327T+F360V, V305M+L327T+F360T, V305M+I408W+F360I, V305M+I408W+F360V, V305L+I408R+F360L, L327T+I408R+F360M, L327T+I408W+F360V, R89A+V165C+A167C+F360I, R89A+V165S+V305M+F360M, R89A+V165C+L327T+F360V, R89A+V165S+I408R+F360L, R89A+A167L+V305L+F360T, R89A+A167L+L327T+F360I, R89A+A167C+I408R+F360M, V165C+A167I+V305M+F360V, V165S+A167C+L327T+F360M, V165C+A167C+I408W+F360T, A167I+V305L+L327T+F360V, A167L+V305M+I408R+F360M, 또는 V305L+L327T+I408W+F360V의 아미노산 변이를 포함하는 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 것일 수 있다.
다른 구체예에서, 상기 폴리펩타이드의 변이체는 서열번호 3의 아미노산 서열에 있어서, F360M, F360V, F360I, F360L, A167C, A167L, A167I, V305M, R89A, V165S, V165C, L327T, R89A+F360M, A167L+F360M, L327T+F360M, R89A+F360V, A167L+F360I, V305M+F360I, R89A+V165C+F360I, V165C+A167L+F360I, V165C+A167L+F360M, A167L+V305M+F360M, V165S+A167L+V305M+F360I, R89A+V165S+V305M+F360I, V165S+A167C+L327T+F360M, R89A+V165S+A167C+L327T+F360M, 또는 R89A+V165C+A167L+V305M+F360I의 아미노산 변이를 포함하는 아미노산 서열 또는 이와 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 것일 수 있다.
본 명세서에 기재된 서열 상동성 (예컨대, 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성)을 갖는 아미노산 서열을 포함하는 폴리펩타이드 또는 폴리펩타이드 변이체는 서열 상동성 판단의 기준이 되는 아미노산 서열을 갖는 폴리펩타이드 (예컨대, 앞서 설명한 아미노산 서열 또는 아미노산 변이를 갖는 PPO 단백질)과 동등 정도의 효소 활성, 예컨대, 식물 내 (식물체 내, 식물 세포 또는 세포 배양체 내, 식물 조직 내 등), 조류 내, 및/또는 시험관내에서 기준이 되는 아미노산 서열을 갖는 폴리펩타이드의 5% 이상, 10% 이상, 20% 이상, 30% 이상, 40% 이상, 50% 이상, 60% 이상, 70% 이상, 80% 이상, 90% 이상, 또는 95% 이상의 효소 활성을 유지하면서 제초제 저항성을 부여하는 기능을 보유하는 것일 수 있으며, 상기와 같은 서열 상동성 기재는 본 명세서에 기재된 제초제 내성 PPO 단백질 (폴리펩타이드) 또는 이의 변이체가 상기 조건 (효소 활성을 유지하면서 제초제 저항성을 부여하는 기능을 보유)을 만족하는 범위의 모든 서열 변이를 포함할 수 있음을 명확하게 하기 위하여 사용된다.
본 명세서에서 사용된 아미노산의 명명을 아래의 표에 정리하였다:
상기 제초제 내성 PPO 단백질 변이체는 야생형의 효소 활성은 유지하면서, 야생형과 비교하여 증진된 제초제 내성을 나타내는 것을 특징으로 한다.
또한, 상기 폴리펩타이드 (제초제 내성 PPO 단백질) 및 폴리펩타이드 변이체 (제초제 내성 PPO 단백질 변이체)는 앞서 설명한 바와 같은 서열번호 1, 서열번호 2, 또는 서열번호 3, 또는 앞서 설명한 바와 같은 아미노산 변이를 갖는 아미노산 서열로 이루어지는 폴리펩타이드와 생물학적 균등한 활성을 나타내는 변이를 추가로 포함할 수 있다. 예컨대, 상기 추가적 변이는 분자의 활성을 전체적으로 변경시키지 않는 아미노산 치환일 수 있고, 이러한 아미노산 치환은 당해 분야에 공지되어 있다. 일 예에서, 상기 추가적 치환은 아미노산 잔기 Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Thr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, 또는 Asp/Gly 간의 치환을 들 수 있으나, 이에 제한되는 것은 아니다. 경우에 따라서, 상기 제초제 내성 PPO 단백질 변이체는 하나 이상의 아미노산이 인산화 (phosphorylation), 황화 (sulfation), 아실화 (acylation), 당화 (glycosylation), 메틸화 (methylation), 파네실화 (farnesylation) 등으로 이루어진 군에서 선택된 하나 이상으로 수식 (modification) 될 수도 있다. 또한, 상기 제초제 내성 PPO 단백질 변이체는 아미노산 변이 및/또는 수식에 의해서 단백질의 열, pH 등에 대한 구조적 안정성이 증가하거나 단백질 활성이 증가한 단백질 변이체를 포함할 수 있다.
용어 "서열 상동성"이란 야생형 (wild type) 또는 기준이 되는 아미노산 서열 또는 염기 서열과의 유사한 정도를 나타내기 위한 것으로서, 제초제 내성 PPO 단백질 변이체의 아미노산 서열과 60% 이상, 65% 이상, 70% 이상, 75% 이상, 80% 이상, 85% 이상, 90% 이상, 95% 이상, 96% 이상, 97% 이상, 98% 이상 또는 99% 이상 동일한 아미노산 잔기를 포함하면서, 상기 제초제 내성 PPO 단백질 또는 이의 변이체와 생물학적으로 균등한 활성을 보유한다면 본 발명의 범위에 포함될 수 있다. 이러한 단백질 상동물은 목적 단백질과 동일한 활성 부위를 포함할 수 있다. 이러한 상동성의 비교는 육안으로나 구입이 용이한 비교 프로그램을 이용하여 수행할 수 있다. 온라인 분석 프로그램은 2개 이상의 서열간의 상동성을 백분율(%)로 계산할 수 있다. 비교를 위한 서열 정렬 방법은 당업계에 알려진 방법을 사용할 수 있으며, 예를 들어, GAP, BESTFIT, BLAST, 및 Clustal Omega 등을 포함한다.
상기 제초제 내성 PPO 단백질 또는 이들의 변이체는 당 분야에 널리 공지된 방법으로 천연에서 추출 및/또는 정제하여 얻을 수 있다. 또는, 유전자 재조합 기술을 이용한 재조합 단백질로 수득할 수 있다. 유전자 재조합 기술을 이용할 경우, 제초제 내성 PPO 단백질 또는 이들의 변이체를 암호화하는 핵산을 적절한 발현 벡터에 삽입하고, 벡터를 숙주세포로 형질전환하여 목적 단백질이 발현되도록 숙주 세포를 배양한 뒤, 숙주세포로부터 제초제 내성 PPO 단백질 또는 이들의 변이체를 회수하는 과정으로 수득할 수 있다. 단백질은 선택된 숙주 세포에서 발현시킨 후, 분리 및 정제를 위해 통상적인 생화학 분리 기술, 예를 들어 단백질 침전제에 의한 처리 (염석법), 원심분리, 초음파 파쇄, 한외여과, 투석법, 분자체 크로마토그래피 (겔여과), 흡착크로마토그래피, 이온교환 크로마토그래피, 친화도 크로마토그래피 등의 각종 크로마토그래피 등을 이용할 수 있으며, 순도가 높은 단백질을 분리하기 위하여 이들 중 2가지 이상을 조합하여 이용할 수 있다.
상기 제초제 내성 PPO 핵산 분자 (PPO 단백질 또는 이들의 변이체를 암호화 하는 폴리뉴클레오타이드)는 표준 분자 생물학 기술, 예를 들어 화학적 합성 방법 또는 재조합 방법을 이용하여 분리 또는 제조하거나, 시판되는 것을 사용할 수 있다.
구체적인 실시예에서, 상기 제공된 PPO 단백질/핵산분자 또는 이들의 변이체들은 PPO가 결핍된 대장균 BT3(ΔPPO)를 이용한 제초제 내성 검증 시스템을 통하여, 화학 구조에 따라 10개 계통에 속하는 대표적인 PPO 활성 저해 제초제들에 대하여 광범위한 제초제 내성을 나타낸다는 것이 검증되었으며, 엽록체 트랜지트 펩타이드 (transit peptide, TP)를 사용하면 식물의 엽록체에서 발현될 수 있다는 것이 확인되었다. 또한, 상기 PPO 단백질/핵산분자는 식물 발현용 벡터에 의하여 애기장대 (Arabidopsis thaliana ecotype Columbia-0)와 같은 쌍자엽 식물 또는 벼와 같은 단자엽 식물에서 발현이 가능하며, 이와 같이 형질전환된 식물체에 PPO 활성 저해 제초제를 처리하더라도 식물이 발아 및/또는 생장 가능한 것이 확인되었다. 아울러, 후대 유전성 시험을 통하여 위와 같은 제초제 내성 형질이 다음 세대로 성공적으로 전달되는 것이 확인되었다.
따라서, 본 명세서에서 제공하는 PPO 단백질 또는 이들의 변이체들을 식물 또는 조류에 도입함으로써 식물 또는 조류의 제초제 내성을 부여 및/또는 증진시키는 데 사용될 수 있다.
일 예는
(1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 앞서 설명한 이들의 폴리펩타이드 변이체, 및 이들과 95% 이상, 96% 이상, 97% 이상, 98% 이상, 또는 99% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드 또는 폴리펩타이드 변이체,
(2) 상기 (1)의 폴리펩타이드 또는 폴리펩타이드 변이체를 암호화하는 폴리뉴클레오타이드,
(3) 상기 (2)의 폴리뉴클레오타이드를 포함하는 재조합 벡터, 및
(4) 상기 (3)의 재조합 벡터를 포함하는 재조합 세포
로 이루어진 군에서 선택된 하나 이상을 포함하는, 식물 및/또는 조류의 제초제에 대한 내성 부여 및/또는 증진용 조성물을 제공한다.
본 명세서에서 제초제란, 식물 또는 조류를 사멸시키거나 방제하거나, 또는 식물 또는 조류의 생장을 불리하게 조정하는 활성 성분을 말한다. 또한, 본원에서 제초제 내성이란, 정상 또는 야생형 식물 또는 조류를 정상적으로 사멸시키거나 그의 생장을 정상적으로 억제시키는 제초제를 처리하더라도, 정상 또는 야생형의 식물 또는 조류에 비하여 식물 또는 조류 생장의 저해 정도가 약화되거나 제거되어, 식물 또는 조류의 생장이 지속적으로 이루어지는 것을 말한다. 상기 제초제는 식물 또는 조류의 프로토포르피리노겐 IX 옥시다아제 (PPO) 를 억제하는 제초제를 포함한다. 이러한 PPO 저해 제초제는 화학 구조에 따라 피리미딘디온계 (pyrimidinediones), 디페닐에테르계 (diphenyl-ethers), 페닐피라졸계 (phenylpyrazoles), 페닐프탈이미드계 (N-phenylphthalimides), 페닐에스테르계 (phenylesters), 티아디아졸계 (thiadiazoles), 옥사디아졸계 (oxadiazoles), 트리아지논계 (triazinones), 트리아졸리논계 (triazolinones), 옥사졸리딘디온계 (oxazolidinediones) 및 기타의 제초제를 예시할 수 있다.
구체예로, 피리미딘디온계 제초제는 부타페나실 (butafenacil), 사플루페나실 (saflufenacil), 벤즈펜디존 (benzfendizone) 및 티아페나실 (tiafenacil)을 포함하나, 이에 제한되지 않는다.
디페닐에테르계 제초제는 포메사펜 (fomesafen), 옥시플루오르펜 (oxyfluorfen), 아클로니펜 (aclonifen), 아시플루오르펜 (acifluorfen), 비페녹스 (bifenox), 에톡시펜 (ethoxyfen), 락토펜 (lactofen), 클로메톡시펜 (chlomethoxyfen), 클로르니트로펜 (chlornitrofen), 플루오로글리코펜-에틸 (fluoroglycofen-ethyl) 및 할로사펜 (halosafen) 을 포함하나, 이에 제한되지 않는다.
페닐피라졸계 제초제는 피라플루펜-에틸 (pyraflufen-ethyl) 및 플루아졸레이트 (fluazolate)를 포함하나, 이에 제한되지 않는다.
페닐프탈이미드계 제초제는 플루미옥사진 (flumioxazin), 시니돈-에틸 (cinidon-ethyl) 및 플루미클로락-펜틸 (flumiclorac-pentyl)을 포함하나, 이에 제한되지 않는다.
페닐에스테르계 (phenylesters) 제초제는 페노필레이트 (phenopylate; 2,4-dichlorophenyl 1-pyrrolidinecarboxylate) 및 페노필레이트의 카바메이트 유사체 (예컨대, O-phenylpyrrolidino- and piperidinocarbamate analoges (“Ujjana B. Nandihalli, Mary V. Duke, Stephen O. Duke, Relationships between molecular properties and biological activities of O-phenyl pyrrolidino- and piperidinocarbamate herbicides., J. Agric. Food Chem., 40(10) 1993-2000, 1992” 참조)) 등을 포함하나, 이에 제한되는 것은 아니다. 일 구체예에서, 상기 페노필레이트의 카바메이트 유사체는 피롤리딘-1카르복실산 페닐 에스테르 (pyrrolidine-1-carboxylic acid phenyl ester; CAS No. 55379-71-0), 1-피롤리딘카르복실산, 2-클로로페닐 에스테르 (1-Pyrrolidinecarboxylicacid, 2-chlorophenyl ester; CAS No. 143121-06-6), 4-클로로페닐 피롤리딘-1-카르복실레이트 (4-chlorophenyl pyrrolidine-1-carboxylate: CAS No. 1759-02-0), 카르밤산, 디에틸-, 2,4-디클로로-5-(2-프로피닐옥시)페닐 에스테르 (carbamic acid, diethyl-, 2,4-dichloro-5-(2-propynyloxy)phenyl ester (9CI); CAS No. 143121-07-7), 1-피롤리딘카르복시산, 2,4-디클로로-5-하이드로페닐 에스테르 (1-pyrrolidinecarboxylicacid, 2,4-dichloro-5-hydroxyphenyl ester; CAS No. 143121-08-8), 2,4-디클로로-5-(메톡시카르보닐)페닐 피롤리딘-1-카르복실레이트 (2,4-dichloro-5-(methoxycarbonyl)phenyl pyrrolidine-1-carboxylate; CAS No. 133636-94-9), 2,4-디클로로-5-[(프로판-2-일옥시)카르보닐]페닐 피롤리딘-1-카르복실레이트 (2,4-dichloro-5-[(propan-2-yloxy)carbonyl]phenyl pyrrolidine-1-carboxylate; CAS No. 133636-96-1), 1-피페리딘카르복실산, 2,4-디클로로-5-(2-프로피닐옥시)페닐 에스테르 (1-Piperidinecarboxylic acid, 2,4-dichloro-5-(2-propynyloxy)phenyl ester; CAS No. 87374-78-5), 2,4-디클로로-5-(프로프-2-인-1-일옥시)페닐 피롤리딘-1-카르복실레이트 (2,4-dichloro-5-(prop-2-yn-1-yloxy)phenyl pyrrolidine-1-carboxylate; CAS No. 87365-63-7), 2,4-디클로로-5-(프로프-2-인-1-일옥시)페닐 4,4-디플루오로피페리딘-1-카르복실레이트 (2,4-dichloro-5-(prop-2-yn-1-yloxy)phenyl 4,4-difluoropiperidine-1-carboxylate; CAS No. 138926-22-4), 1-피롤리딘카르복실산, 3,3-디클루오로-, 2,4-디클로로-5-(2-프로핀-1-일옥시)페닐 에스테르 (1-pyrrolidinecarboxylicacid, 3,3-difluoro-, 2,4-dichloro-5-(2-propyn-1-yloxy)phenyl ester; CAS No. 143121-10-2), 4-클로로-2-플루오로-5-[(프로판-2-일옥시)카르보닐]페닐 피롤리딘-1-카르복실레이트 (4-chloro-2-fluoro-5-[(propan-2-yloxy)carbonyl]phenyl pyrrolidine-1-carboxylate; CAS No. 133636-98-3) 등으로 이루어진 군에서 선택된 하나 이상일 수 있다.
티아디아졸계 제초제는 플루티아셋 (fluthiacet) 및 티디아지민(Thidiazimin)을 포함하나, 이에 제한되지 않는다.
옥사디아졸계 제초제는 옥사디아길 (oxadiargyl) 및 옥사디아존(Oxadiazon)을 포함하나, 이에 제한되지 않는다.
트리아지논계 제초제는 트리플루디목사진(trifludimoxazin)을 포함하나, 이에 제한되지 않는다.
트리아졸리논계 제초제는 카펜트라존 (carfentrazone), 설펜트라존 (sulfentrazone) 및 아자페니딘 (azafenidin)을 포함하나, 이에 제한되지 않는다.
옥사졸리딘디온계 제초제는 펜톡사존 (pentoxazone)을 포함하나, 이에 제한되지 않는다.
기타의 제초제는 피라클로닐 (pyraclonil), 플루펜피르-에틸 (flufenpyr-ethyl) 및 프로플루아졸 (profluazol)을 포함하나, 이에 제한되지 않는다.
본원에서 제공하는 제초제 내성 PPO 유전자 또는 이의 변이체는 당업계에 공지된 다양한 방법에 의하여 식물체 또는 조류 내에 도입될 수 있으며, 바람직하게는 식물 또는 조류 형질전환용 발현벡터가 이용될 수 있다.
식물체 형질전환의 경우, 벡터에 포함되는 적합한 프로모터로는, 식물체 내 유전자 도입을 위해 당업계에서 통상적으로 이용되는 어떠한 것도 이용될 수 있으며, 예를 들어, SP6 프로모터, T7 프로모터, T3 프로모터, PM 프로모터, 옥수수의 유비퀴틴 프로모터, 컬리플라워 모자이크 바이러스 (CaMV) 35S 프로모터, 노팔린 씬타아제 (nos) 프로모터, 피그워트 모자이크 바이러스 35S 프로모터, 수가크레인 바실리폼 바이러스 프로모터, 콤멜리나 엘로우 모틀 바이러스 프로모터, 리불로오스-1,5-비스-포스페이트 카르복실라아제 스몰 서브유니트 (ssRUBISCO)의 광유도성 프로모터, 벼 사이토졸 트리오스포스페이트 이소머라아제 (TPI) 프로모터, 아라비돕시스의 아데닌 포스포리보실트랜스퍼라아제 (APRT) 프로모터, 옥토파인 신타아제 프로모터 및 BCB (blue copper binding protein) 프로모터 등으로 이루어진 군에서 선택된 하나 이상을 사용할 수 있으나, 이에 제한되는 것은 아니다.
또한, 상기 벡터는 3'-말단의 폴리아데닐화를 야기시키는 폴리 A 시그널서열을 포함할 수 있으며, 예를 들어, 아그로박테리움 투메파시엔스의 노팔린 신타아제 유전자로부터 유래된 것 (NOS 3' end), 아그로박테리움 투메파시엔스의 옥토파인 신타아제 유전자로부터 유래된 터미네이터 (octopine synthase terminator), 토마토 또는 감자의 프로테아제 억제자 I 또는 Ⅱ 유전자의 3' 말단 부분, CaMV 35S 터미네이터, 벼 α-아밀라아제 RAmy1 A 터미네이터 및 파세올린 (phaseoline) 터미네이터를 포함할 수 있으나, 이에 제한되는 것은 아니다.
또한, 조류 형질전환의 경우, 프로모터로서 엽록체 특이적 프로모터, 핵 프로모터, 항시성 프로모터 또는 유도성 프로모터를 사용할 수 있다. 본 명세서에서 제공되는 제초제 내성 PPO 유전자 또는 이의 변이체는 5'UTR 또는 3'UTR 에 작동적으로 연결되어 조류의 핵 안에서 기능을 발휘하도록 설계될 수 있다. 또한, 상기 벡터는 조류 형질전환에 적합한 전사 조절 서열을 더욱 포함할 수 있다. 제초제 내성을 부여하는 재조합 유전자는 숙주 조류에서 핵의 게놈 또는 엽록체의 게놈에 통합될 수 있으나, 이에 제한되는 것은 아니다.
또한, 상기 벡터는 제초제 내성 PPO 유전자 또는 이의 변이체를 엽록체에 발현시키기 위하여, 엽록체로의 타겟팅에 필요한 트랜지트 펩타이드를 PPO 유전자 또는 이의 변이체의 5'-위치에 연결시킬 수 있다.
또한, 상기 벡터는 선택적으로, 리포터 분자로서 선택 표지를 암호화하는 유전자를 추가적으로 포함할 수 있으며, 선택 표지의 예로서 항생제 (예: 네오마이신, 카베니실린, 카나마이신, 스펙티노마이신, 하이그로마이신, 블레오마이신, 앰피실린, 클로람페니콜 등) 또는 제초제 (글리포세이트, 글루포시네이트, 포스피노트리신 등) 내성 유전자 등을 포함할 수 있으나, 이에 제한되는 것은 아니다.
또한, 식물 발현용 재조합 벡터는 아그로박테리움 (Agrobacterium) 바이너리 벡터, 코인테그레이션 벡터 (cointegration vector) 또는 T-DNA 부위를 포함하지는 않지만 식물에서 발현될 수 있도록 디자인된 일반 벡터가 사용될 수 있다. 이 중, 바이너리 벡터는 Ti (tumor inducible) 플라스미드에서 이동에 필요한 부분인 LB (left border)와 RB (right border)를 가지는 플라스미드와 타겟 뉴클레오타이드를 옮기는데 필요한 유전자를 가진 플라스미드를 포함하는 벡터를 말하며, 그 내부에 식물체에서 발현시키기 위한 프로모터 부위와 폴리아데닐화 신호 서열을 포함하는 벡터를 사용할 수 있다.
상기에서 바이너리 벡터 또는 코인테그레이션 벡터를 사용하는 경우, 식물체에 상기 재조합 벡터를 도입하기 위한 형질전환용 균주로는 아그로박테리움을 사용하는 것이 바람직하며 (Agrobacterium-mediated transformation), 이때 아그로박테리움 투메파시엔스 (Agrobacterium tumefaciens) 또는 아그로박테리움 라이조게네스 (Agrobacterium rhizogenes)를 사용할 수 있다. 그 밖에 T-DNA 부위를 포함하지 않는 벡터를 이용하는 경우에는, 전기천공법 (electroporation), 입자 총법 (particle bombardment), 폴리에틸렌 글리콜 침전법 (polyethylene glycol-mediated uptake) 등이 재조합 플라스미드를 식물체로 도입하는데 이용될 수 있다.
상기와 같은 방법으로 유전자가 도입된 형질전환 식물들은 당업계에 공지된 표준 기술을 사용하여 캘러스 유도, 발근 및 토양 순화와 같은 과정을 거쳐 식물체로 재분화시킬 수 있다.
본 명세서에서 형질전환의 대상이 되는 식물은, 성숙한 식물체뿐만 아니라 성숙한 식물로 발육할 있는 식물세포 (현탁배양 세포 포함), 원형질체 (protoplast), 캘러스 (callus), 배축 (hypocotyl), 종자 (seed), 자엽 (cotyledon), 신초 (shoot) 등을 모두 포함하는 의미로서 이해된다.
또한, 본 명세서의 형질전환체의 범주에는 상기 유전자가 도입된 형질전환체 뿐만 아니라 이의 클론 또는 자손 (T1 세대, T2 세대, T3 세대, T4 세대, T5 세대, T6 세대, T7 세대, T8 세대, 또는 그 이상)을 포함하며, 예를 들어, PPO 유전자 또는 이의 변이체가 형질전환된 식물의 무성 또는 유성 자손으로서 제초제 내성 형질이 유전된 식물들도 본 발명의 형질전환 식물의 범주에 포함된다. 또한, 본원의 유전자가 형질전환된 식물의 모든 교배 및 융합 생성물과 함께, 초기 형질전환된 식물의 특성을 나타내는 모든 돌연변이체 및 변이체가 본 발명의 범주에 포함된다. 아울러, 본 발명의 방법으로 미리 형질전환시킨 형질전환된 식물, 또는 이들의 자손으로부터 기원하며 형질전환된 세포의 적어도 일부로 이루어진 종자, 꽃, 줄기, 과실, 잎, 뿌리, 괴경, 및/또는 괴근과 같은 식물의 일부도 본 발명의 범주에 포함된다.
본 발명이 적용될 수 있는 식물은 특별히 제한되지 않으며, 단자엽 또는 쌍자엽 식물을 모두 포함하여 이루어진 군에서 선택된 하나 이상일 수 있다. 또한 초본 또는 목본 식물일 수 있다. 상기 단자엽 식물은 택사과 (Alismataceae), 자라풀과 (Hydrocharitaceae), 지채과 (Juncaginaceae), 장지채과 (Scheuchzeriaceae), 가래과 (Potamogetonaceae), 나자스말과 (Najadaceae), 거머리말과 (Zosteraceae), 백합과 (Liliaceae), 지모과 (Haemodoraceae), 용설란과 (Agavaceae), 수선화과 (Amaryllidaceae), 마과 (Dioscoreaceae), 물옥잠과 (Pontederiaceae), 붓꽃과 (Iridaceae), 버먼초과 (Burmanniaceae), 골풀과 (Juncaceae), 닭의장풀과 (Commelinaceae), 곡정초과 (Eriocaulaceae), 화본과 (벼과, Gramineae, Poaceae), 천남성과 (Araceae), 개구리밥과 (Lemnaceae), 흑삼릉과 (Sparganiaceae), 부들과 (Typhaceae), 사초과 (방동사니과, Cyperaceae), 파초과 (Musaceae), 생강과 (Zingiberaceae), 홍초과 (Cannaceae), 난초과 (Orchidaceae)의 식물을 포함할 수 있으나, 이에 제한되는 것은 아니다.
상기 쌍자엽 식물은 암매과 (돌매화나무과, Diapensiaceae), 매화오리나무과 (Clethraceae), 노루발과 (Pyrolaceae), 진달래과 (Ericaceae), 자금우과 (Myrsinaceae), 앵초과 (Primulaceae), 갯질경이과 (Plumbaginaceae), 감나무과 (Ebenaceae), 때죽나무과 (Styracaceae), 노린재나무과 (Symplocaceae), 회목과 (Symplocaceae), 물푸레나무과 (목서과, Oleaceae), 마전과 (Loganiaceae), 용담과 (Gentianaceae), 조름나물과 (Menyanthaceae), 협죽도과 (마삭나무과, Apocynaceae), 박주가리과 (Asclepiadaceae), 꼭두서니과 (Rubiaceae), 꽃고비과 (Polemoniaceae), 메꽃과 (Convolvulaceae), 지치과 (Boraginaceae), 마편초과 (Verbenaceae), 꿀풀과 (Labiatae), 가지과 (Solanaceae), 현삼과 (Scrophulariaceae), 능소화과 (Bignoniaceae), 쥐꼬리망초과 (Acanthaceae), 참깨과 (Pedaliaceae), 열당과 (Orobanchaceae). 제스네리아과 (Gesneriaceae), 통발과 (Lentibulariaceae), 파리풀과 (Phrymaceae), 질경이과 (Plantaginaceae), 인동과 (Caprifoliaceae), 연복초과 (Adoxaceae), 마타리과 (Valerianaceae), 산토끼꽃과 (Dipsacaceae), 초롱꽃과 (Campanulaceae), 국화과 (Compositae), 소귀나무과 (Myricaceae), 가래나무과 (Juglandaceae), 버드나무과 (Salicaceae), 자작나무과 (Betulaceae), 너도밤나무과 (참나무과, Fagaceae), 느릅나무과 (Ulmaceae), 뽕나무과 (Moraceae), 쐐기풀과 (Urticaceae), 단향과 (Santalaceae), 겨우살이과 (Loranthaceae), 마디풀과 (여뀌과, Polygonaceae), 자리공과 (상륙과, Phytolaccaceae), 분꽃과 (Nyctaginaceae), 석류풀과 (Aizoaceae), 쇠비름과 (Portulacaceae), 석죽과 (Caryophyllaceae), 명아주과 (Chenopodiaceae), 비름과 (Amaranthaceae), 선인장과 (Cactaceae), 목련과 (Magnoliaceae), 붓순나무과 (Illiciaceae), 녹나무과 (Lauraceae), 계수나무과 (Cercidiphyllaceae), 미나리아재비과 (Ranunculaceae), 매자나무과 (Berberidaceae), 으름덩굴과 (Lardizabalaceae), 새모래덩굴과 (방기과, Menispermaceae), 수련과 (Nymphaeaceae), 붕어마름과 (Ceratophyllaceae), 어항마름과 (Cabombaceae), 삼백초과 (Saururaceae), 후추과 (Piperaceae), 홀아비꽃대과 (Chloranthaceae), 쥐방울덩굴과 (Aristolochiaceae), 다래나무과 (Actinidiaceae), 차나무과 (동백나무과, Theaceae), 물레나물과 (Guttiferae), 끈끈이주걱과 (Droseraceae), 양귀비과 (Papaveraceae), 풍접초과 (Capparidaceae), 십자화과 (겨자과, Cruciferae), 플라타너스과 (버즘나무과, Platanaceae), 조록나무과 (금루매과, Hamamelidaceae), 꿩의비름과 (돌나물과, Crassulaceae), 범의귀과 (Saxifragaceae), 두충과 (Eucommiaceae), 돈나무과 (Pittosporaceae), 장미과 (Rosaceae), 콩과 (Leguminosae), 괭이밥과 (Oxalidaceae), 쥐손이풀과 (Geraniaceae), 한련과 (Tropaeolaceae), 남가새과 (Zygophyllaceae), 아마과 (Linaceae), 대극과 (Euphorbiaceae), 별이끼과 (Callitrichaceae), 운향과 (Rutaceae), 소태나무과 (Simaroubaceae), 멀구슬나무과 (Meliaceae), 원지과 (Polygalaceae), 옻나무과 (Anacardiaceae), 단풍나무과 (단풍과, Aceraceae), 무환자나무과 (Sapindaceae), 칠엽수과 (Hippocastanaceae), 나도 밤나무과 (Sabiaceae), 봉선화과 (물봉선과, Balsaminaceae), 감탕나무과 (Aquifoliaceae), 노박덩굴과 (화살나무과, Celastraceae), 고추나무과 (Staphyleaceae), 회양목과 (Buxaceae), 시로미과 (Empetraceae), 갈매나무과 (Rhamnaceae), 포도과 (Vitaceae), 담팔수과 (Elaeocarpaceae), 피나무과 (Tiliaceae), 아욱과 (Malvaceae), 벽오동과 (Sterculiaceae), 팥꽃나무과 (서향나무과, Thymelaeaceae), 보리수나무과 (Elaeagnaceae), 이나무과 (Flacourtiaceae), 제비꽃과 (Violaceae), 시계꽃과 (Passifloraceae), 위성류과 (Tamaricaceae), 물별과 (Elatinaceae), 베고니아과 (Begoniaceae), 박과 (Cucurbitaceae), 부처꽃과 (배롱나무과, Lythraceae), 석류나무과 (Punicaceae), 바늘꽃과 (Onagraceae), 개미탑과 (Haloragaceae), 박쥐나무과 (Alangiaceae), 층층나무과 (산수유나무과, Cornaceae), 두릅나무과 (오갈피나무과, Araliaceae), 산형과 (미나리과)(Umbelliferae(Apiaceae))의 식물을 포함할 수 있으나, 이에 한정되는 것은 아니다.
구체예로, 상기 식물은 벼, 밀, 보리, 옥수수, 대두, 감자, 팥, 귀리 및 수수를 포함하는 식량 작물류; 애기장대, 배추, 무, 고추, 딸기, 토마토, 수박, 오이, 양배추, 참외, 호박, 파, 양파 및 당근을 포함하는 채소 작물류; 인삼, 담배, 목화, 마초, 목초, 참깨, 사탕수수, 사탕무우, 들깨, 땅콩, 유채, 잔디 및 피마자를 포함하는 특용 작물류; 사과나무, 배나무, 대추나무, 복숭아, 양다래, 포도, 감귤, 감, 자두, 살구 및 바나나를 포함하는 과수류; 소나무, 팜오일 및 유칼립투스를 포함하는 목본류; 장미, 글라디올러스, 거베라, 카네이션, 국화, 백합 및 튤립을 포함하는 화훼류; 및 라이그라스, 레드클로버, 오차드그라스, 알팔파, 톨페스큐 및 페레니얼라이그라스를 포함하는 사료 작물류 등으로 이루어진 군에서 선택된 하나 이상일 수 있으나, 이에 제한되지 않는다. 구체 예로, 상기 식물은 애기장대, 감자, 가지, 담배, 고추, 토마토, 우엉, 쑥갓, 상추, 도라지, 시금치, 근대, 고구마, 샐러리, 당근, 미나리, 파슬리, 배추, 양배추, 갓무, 수박, 참외, 오이, 호박, 박, 딸기, 대두, 녹두, 강낭콩, 또는 완두 등의 쌍자엽 식물; 및 벼, 밀, 보리, 옥수수, 수수 등의 단자엽 식물 등으로 이루어진 군에서 선택된 하나 이상을 들 수 있으나, 이에 제한되는 것은 아니다.
본 발명이 적용될 수 있는 조류는 특별히 제한되지 않으며, 원핵 (Prokaryotic) 조류 또는 진핵 (Eukaryotic) 조류일 수 있다. 예를 들어, 조류는 시아노박테리아, 녹조류, 홍조류, 갈조류, 대형조류 (macroalgae) 또는 미세조류 (microalgae)일 수 있다.
시아노박테리아류로는, Chroococcales 문 (예, Aphanocapsa, Aphanothece, Chamaesiphon, Chondrocystis, Chroococcus, Chroogloeocystis, Crocosphaera, Cyanobacterium, Cyanobium, Cyanodictyon, Cyanosarcina, Cyanothece, Dactylococcopsis, Gloeocapsa, Gloeothece, Halothece, Johannesbaptistia, Merismopedia, Microcystis, Radiocystis, Rhabdoderma, Snowella, Synechococcus, Synechocystis, Thermosynechococcus, Woronichinia), Gloeobacteria 문, Nostocales 문 (예, Microchaetaceae, Nostocaceae, Rivulariaceae, Scytonemataceae), Oscillatoriales 문 (예, Arthronema, Arthrospira, Blennothrix, Crinalium, Geitlerinema, Halomicronema, Halospirulina, Hydrocoleum, Jaaginema, Katagnymene, Komvophoron, Leptolyngbya, Limnothrix, Lyngbya, Microcoleus,Oscillatoria, Phormidium, Planktothricoides, Planktothrix, Plectonema, Pseudanabaena, Pseudophormidium, Schizothrix, Spirulina, Starria, Symploca, Trichodesmium, Tychonema), Pleurocapsales 문 (예, Chroococcidiopsis, Dermocarpa, Dermocarpella, Myxosarcina, Pleurocapsa, Solentia, Stanieria, Xenococcus), Prochlorales 문, 또는 Stigonematales 문 (예, Capsosira, Chlorogloeopsis, Fischerella, Hapalosiphon, Mastigocladopsis, Mastigocladus, Nostochopsis, Stigonema, Symphyonema, Symphonemopsis, Umezakia, Westiellopsis) 등을 예시할 수 있다.
조류의 다른 예로, Chlorophyta, Chlamydomonas, Volvocales, Dunaliella, Scenedesmus, Chlorella, 또는 Hematococcm 를 예시할 수 있다.
조류의 다른 예로, Phaeodactylum tricornutum, Amphiprora hyaline, Amphora spp., Chaetoceros muelleri, Navicula saprophila, Nitzschia communis, Scenedesmus dimorphus, Scenedesmus obliquus, Tetraselmis suecica, Chlamydomonas reinhardtii, Chlorella vulgaris, Haematococcus pluvialis, Neochloris oleoabundans, Synechococcus elongatus, Botryococcus braunii, Gloeobacter violaceus, Synechocystis, Thermosynechococcus elongatus, Nannochloropsis oculata, Nannochloropsis salina, Nannochloropsis gaditana, Isochrysis galbana, Botryococcus sudeticus, Euglena gracilis, Neochloris oleoabundans, Nitzschia palea, Pleurochrysis carterae, Tetraselmis chuii, Pavlova spp., Aphanocapsa spp., Synechosystis spp., Nannochloris spp. 등을 예시할 수 있다. 그러나, 상기 열거한 종류들에 제한 되는 것은 아니며, 그 외 다양한 속 및 종에 속하는 조류들이 포함될 수 있다.
본 명세서에서 제공되는 제초제 내성 PPO 또는 이의 변이체가 도입된 식물 또는 조류는 2 종 이상의 PPO 저해 제초제에 대하여 내성을 나타낼 수 있다.
따라서, 본 명세서에서 제공되는 기술은 2 종 이상의 PPO 저해 제초제를 순차적으로, 또는 동시에 사용함으로써 잡초를 방제하거나 원하지 않는 수생 생물을 제거하는 데 사용될 수 있다.
일 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자를 포함하는 식물을 재배지에 제공하는 단계, 및 상기 재배지에 2종 이상의 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 순차적으로 또는 동시에 적용하는 단계를 포함하는, 재배지에서 잡초를 방제하는 방법을 제공한다.
다른 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자를 포함하는 조류를 배양 배지에 제공하는 단계, 및 상기 배양 배지에 2종 이상의 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 순차적으로 또는 동시에 적용하는 단계를 포함하는, 배양 배지에서 원하지 않는 수생 생물들을 제거하는 방법을 제공한다.
또한, 본 명세서에서 제공하는 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자는, 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자와 조합하여 사용될 수 있다.
이에 따라, 본 명세서에서 제공하는 제초제 내성 PPO 또는 이의 변이체가 도입된 식물 또는 조류는 작용기전이 상이한 2 종 이상의 제초제에 대하여 내성을 나타낼 수 있다. 따라서, 본 발명은 작용기전이 PPO 저해 제초제를 포함한 상이한 2 종 이상의 제초제를 순서에 상관없이 순차적으로, 또는 동시에 사용함으로써 잡초를 방제하거나 및/또는 원치 않는 수생 생물을 제거하는 데 사용될 수 있다. 이하, PPO 저해 제초제와 작용기전이 상이한 제초제를 "제2의 제초제"라고 지칭한다.
일 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자; 및 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 포함하는, 식물 또는 조류의 제초제에 대한 내성 부여 및/또는 증진용 조성물을 제공한다.
다른 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자; 및 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 포함하는, 식물 또는 조류의 제초제에 대한 내성을 가지는 형질전환체, 이의 클론 또는 자손을 제공한다.
다른 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자; 및 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 조류, 또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초 또는 식물체 전체에 형질전환하는 단계를 포함하는, 제초제에 대한 내성을 가지는 식물 또는 조류를 제조하는 방법을 제공한다.
다른 예는 상술한 제초제 내성 PPO 단백질, 이의 변이체, 또는 이를 암호화하는 유전자; 및 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 포함하는 식물을 재배지에 제공하는 단계, 및 상기 재배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제 및 제2의 제초제의 유효량을 동시에 또는 순서에 상관없이 순차적으로 적용하는 단계를 포함하는, 재배지에서 잡초를 방제하는 방법을 제공한다.
다른 예는 상술한 제초제 내성 PPO 단백질 또는 이를 암호화하는 유전자; 및 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 포함하는 조류를 배양 배지에 제공하는 단계, 및 상기 배양 배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제 및 제2의 제초제의 유효량을 동시에 또는 순서에 상관없이 순차적으로 적용하는 단계를 포함하는, 배양 배지에서 원하지 않는 수생 생물을 제거하는 방법을 제공한다.
예컨대, 상기 식물 또는 조류는 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하며 상기 제2의 제초제에 대한 내성이 부여 및/또는 증진된 것일 수 있다.
예컨대, 상기 제2의 제초제는 세포분열 저해성 제초제, 광합성 저해성 제초제, 아미노산합성 저해성 제초제, 색소체 저해성 제초제, 세포막 저해성 제초제 및/또는 이들의 조합을 포함하나, 이에 제한되는 것은 아니다. 구체 예로, 상기 제2의 제초제는 글리포세이트 (glyphosate), 글루포시네이트 (glufosinate), 디캄바 (dicamba), 2,4-D (2,4-Dichlorophenoxyacetic acid), ALS (acetolactate synthase) 저해성 제초제 (예를 들어, 이미다졸리디논, 설포닐우레아, 트리아졸피리미딘, 설포아닐라이드, 피리미딘 티오벤조에이트 등), 제2광계 (photosystem II) 저해성 제초제, 페닐우레아 (phenylurea)계 제초제, 색소체 저해성 제초제, 브로목시닐 (bromoxynil)계 제초제 및/또는 이들의 조합을 예시할 수 있으나, 이에 제한되는 것은 아니다.
예컨대, 제2의 제초제 내성 폴리펩타이드는 글리포세이트 제초제 내성 EPSPS (glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase), GOX (glyphosate oxidase), GAT (glyphosate-N-acetyltransferase) 또는 글리포세이트 디카복실레이즈 (glyphosate decarboxylase); 글루포시네이트 제초제 내성 PAT (phosphinothricin-N-acetyltransferase); 디캄바 제초제 내성 DMO (dicamba monooxygenase); 2,4-D 제초제 내성 2,4-D 모노옥시게나아제 또는 AAD (aryloxyalkanoate Dioxygenase); ALS 저해성 설포닐우레아 (sulfonylurea)계 제초제 내성 ALS (acetolactate synthase), AHAS (acetohydroxyacid synthase), 또는 AtAHASL (acetohydroxyacid synthase large subunit); 제2광계 저해성 제초제 내성 광계 II (photosystem II) 단백질 D1; 페닐우레아계 제초제 내성 시토크롬 P450 (cytochrome P450); 색소체 저해성 제초제 내성 HPPD (hydroxyphenylpyruvate dioxygenase); 브로목시닐 제초제 내성 니트릴레이즈 (nitrilase); 및 이들의 조합으로 이루어진 군에서 선택되는 1종 이상을 예시할 수 있으나, 이에 제한되는 것은 아니다.
또한, 상기 제2의 제초제 내성 폴리펩타이드를 암호화하는 유전자는 글리포세이트 제초제 내성 cp4 epsps, epsps (AG), mepsps, 2mepsps, goxv247, gat4601 또는 gat4621 유전자; 글루포시네이트 제초제 내성 bar, pat 또는 pat (SYN) 유전자; 디캄바 제초제 내성 dmo 유전자; 2,4-D 제초제 내성 AAD-1, AAD-12 유전자; ALS 저해성 설포닐우레아계 제초제 내성 ALS, GM-HRA, S4-HRA, ZM-HRA, Csr1, Csr1-1, Csr1-2, SurA 또는 SurB 유전자; 제2광계(photosystem II) 저해성 제초제 내성 psbA 유전자; 페닐우레아 제초제 내성 CYP76B1 유전자; 이속사플루톨 제초제 내성 HPPDPF W336 유전자; 브로목시닐 제초제 내성 bxn 유전자; 및 이들의 조합으로 이루어진 군에서 선택되는 1종 이상을 예시할 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서 제공하는 제초제 내성 PPO 단백질의 변이체 또는 이를 암호화하는 유전자를 식물 또는 조류에 적용함으로써 보다 우수한 제초제 내성 형질을 부여 및/또는 증진시키고, 제초제를 이용한 선별적 방제를 통해 경제적으로 잡초를 방제하거나 원하지 않는 수생 생물을 제거할 수 있다.
도 1은 pMAL-c2X 벡터의 개열지도이다.
도 2는 pET303-CT-His벡터의 개열지도이다.
도 3 내지 도 30은 PPO가 결핍된 BT3 대장균 (ΔPPO)에 CyPPO19의 야생형 유전자 (CyPPO19 WT로 나타냄) 또는 다양한 CyPPO19 변이 유전자를 형질전환시킨 후, 다양한 제초제를 다양한 농도로 처리한 경우의 세포 생장 정도를 보여주는 사진이다.
도 31 및 도 32는 BT3(ΔPPO)에 CyPPO18의 야생형 유전자 (CyPPO18 WT로 나타냄) 또는 다양한 CyPPO18 변이 유전자를 형질전환시킨 후, 다양한 제초제를 다양한 농도로 처리한 경우의 세포 생장 정도를 보여주는 사진이다.
도 33은 MBP (maltose binding protein)과 PPO 단백질이 융합된 단백질 제조를 위한 재조합 벡터를 모식적으로 보여주는 그림이다.
도 34 및 도 35는 CyPPO19 WT, CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 또는 1μM flumioxazin 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 36 및 도 37은 CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자로 형질전환된 애기장대 (T2)의 5μM tiafenacil 또는 5μM flumioxazin 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 38은 CyPPO18 WT 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 39는 CyPPO18 변이 (L327T+F360M) 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 2는 pET303-CT-His벡터의 개열지도이다.
도 3 내지 도 30은 PPO가 결핍된 BT3 대장균 (ΔPPO)에 CyPPO19의 야생형 유전자 (CyPPO19 WT로 나타냄) 또는 다양한 CyPPO19 변이 유전자를 형질전환시킨 후, 다양한 제초제를 다양한 농도로 처리한 경우의 세포 생장 정도를 보여주는 사진이다.
도 31 및 도 32는 BT3(ΔPPO)에 CyPPO18의 야생형 유전자 (CyPPO18 WT로 나타냄) 또는 다양한 CyPPO18 변이 유전자를 형질전환시킨 후, 다양한 제초제를 다양한 농도로 처리한 경우의 세포 생장 정도를 보여주는 사진이다.
도 33은 MBP (maltose binding protein)과 PPO 단백질이 융합된 단백질 제조를 위한 재조합 벡터를 모식적으로 보여주는 그림이다.
도 34 및 도 35는 CyPPO19 WT, CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 또는 1μM flumioxazin 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 36 및 도 37은 CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자로 형질전환된 애기장대 (T2)의 5μM tiafenacil 또는 5μM flumioxazin 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 38은 CyPPO18 WT 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
도 39는 CyPPO18 변이 (L327T+F360M) 유전자로 형질전환된 애기장대 (T2)의 1μM tiafenacil 처리 7일째의 관찰 결과를 야생형 애기장대 (Col-0)와 비교하여 보여주는 사진이다.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명이 하기 실시예에 의해 한정되는 것은 아니다.
실시예 1. 원핵생물로부터 PPO 유전자의 분리
써모시네코코커스 에롱가투스 PKUAC-SCTE542 (Thermosynechococcus elongatus PKUAC-SCTE542), 시아노박테리아 박테리움 J003 (Cyanobacteria bacterium J003), 및 써모시네코코커스 불카누스 NIES-2134 (Thermosynechococcus vulcanus NIES-2134)의 Genbank database를 이용하여 PPO 유전자정보를 획득하여 각 PPO 유전자를 합성하였다 (Integrated DNA Technologies). 이를, pMAL-c2X 벡터 (도 1 참조)에 클로닝하기 위해 표 1의 프라이머를 이용, 아래의 조건 (표 2)으로 PPO 유전자를 증폭하였다.
PCR 반응액 조건
Template (CyPPO19, CyPPO20, 및 CyPPO18의 합성 DNA) 1 ㎕
10X buffer 5 ㎕
dNTP mixture (각 10 mM each) 1 ㎕
Forward primer (10 μM, 표1 참조) 1 ㎕
Reverse primer (10 μM, 표1 참조) 1 ㎕
DDW 40 ㎕
Pfu-X (Solgent, 2.5 units/㎕) 1 ㎕
Total 50 ㎕
써모시네코코커스 에롱가투스 PKUAC-SCTE542 균주에서 분리한 PPO를 CyPPO19로, 시아노박테리아 박테리움 J003 균주에서 분리한 PPO를 CyPPO20, 써모시네코코커스 불카누스 NIES-2134 균주에서 분리한 PPO를 CyPPO18로 각각 명명하였다.
균주 | 프라이머 | 서열 | 서열번호 |
Thermosynechococcus elongatus PKUAC-SCTE542 | Cy19 BglII_F | GAAGATCTATGAGTGAGGTAGATGTCG | 159 |
Cy19 SalI_R | ACGCGTCGACCTAGGGCTGGCCTCCTGA | 160 | |
Cyanobacteria bacterium J003 | Cy20 BglII_F | GAAGATCTATGATGGAGGTAGATGTCGC | 161 |
Cy20 SalI_R | ACGCGTCGACTTAACCTCCTGAAAGGTAGGC | 162 | |
Thermosynechococcus vulcanus NIES-2134 | Cy18 BglII_F | CCAGATCTATGATTGAGGTAGATGTCG | 163 |
Cy18 SalI_R | CCGTCGACCTAGGACTGGCCTCCTGC | 164 |
94 ℃ | 4분 | 1 cycle |
94 ℃ | 30초 | 25 cycles |
56 ℃ | 30초 | |
72 ℃ | 1.5분 | |
72 ℃ | 5분 | 1 cycle |
4 ℃ | 5분 | 1 cycle |
실시예
2.
PPO
변이체의
제조
CyPPO19, CyPPO20, 및 CyPPO18의 PPO 저해 제초제 내성을 보다 높이기 위하여 각각 PPO 아미노산 서열 중 제초제와 상호작용하는 위치의 아미노산에 변이를 유발하여 CyPPO19, CyPPO20, 및 CyPPO18의 변이 유전자를 제작하였다.
구체적인 시험 과정은 다음과 같다:
표 3의 프라이머를 이용, 표 4 조건 하에서 PCR 을 수행하여 PPO 유전자를 분리 및 증폭시켰다:
PCR 반응액 조건
Template (CyPPO19, CyPPO20, 및 CyPPO18의 합성 DNA) 1 ㎕
10X buffer 5 ㎕
dNTP mixture (각 10 mM) 1 ㎕
forward primer (10 uM) 1 ㎕
reverse primer (10 uM) 1 ㎕
DDW 40μl
Pfu-X (Solgent, 2.5unit/㎕) 1 ㎕
Total 50 ㎕
Strain | Primer | Sequence | 서열번호 |
Thermosynechococcus elongatus PKUAC-SCTE542 | Cy19 XbaI_F | AGTATCTAGAATGAGTGAGGTAGATGTCG | 165 |
Cy19 XhoI_R | TTA ACTCGAGGGGCTGGCCTCCTGAAAG | 166 | |
Cyanobacteria bacterium J003 | Cy20 XbaI_F | GCTCTAGAATGATGGAGGTAGATGTCGC | 167 |
Cy20 XhoI_R | CCGCTCGAGACCTCCTGAAAGGTAGGC | 168 | |
Thermosynechococcus vulcanus NIES-2134 | Cy18 XbaI_F | CCTCTAGAATGATTGAGGTAGATGTCG | 169 |
Cy18 XhoI_R | CCCTCGAGGGACTGGCCTCCTGCAAGA | 170 |
상기 증폭한 유전자 products와 pET303-CT His vector (Invitrogen; 도 2 참조)를 XbaI, XhoI 으로 절단한 후, T4 DNA ligase(RBC, 3unit/㎕)를 이용하여 pET303-CyPPO19과 pET303-CyPPO20, pET303-CyPPO18 plasmid를 각각 제작하였다.
상기 pET303-CT His vector에 클로닝한 CyPPO19과 CyPPO20, CyPPO18을 template으로 하여, 아래 표5 내지 표7의 프라이머를 사용하여 다음의 조건 하 에서 PCR을 수행하여 CyPPO19과 CyPPO20, CyPPO18의 변이 유전자를 제작하였다.
PCR 반응액 조건
Template 1 ㎕
10X buffer 5 ㎕
dNTP mixture (각 10 mM) 1 ㎕
forward primer (10 μM) 1 ㎕
reverse primer (10 μM) 1 ㎕
DDW 40 ㎕
Pfu-X (Solgent, 2.5unit/μl) 1 ㎕
Total 50 ㎕
94 ℃ | 4분 | 1 cycle |
94 ℃ | 30초 | 17~25cycles |
56~60 ℃ | 30초 | |
72 ℃ | 3분 | |
72 ℃ | 5분 | 1 cycle |
4 ℃ | 5분 | 1 cycle |
CyPPO19의 아미노산 변이 | 서열 (5'-> 3') | 서열번호 |
Cy19 R89A F | GATCGCCACCTGCCGGCGTACATCTACTGGCGG | 171 |
Cy19 R89A R | CCGCCAGTAGATGTACGCCGGCAGGTGGCGATC | 172 |
Cy19 V165C F | GTCTCTGGGtgcTATGCGGGTGATCCACAACAA | 173 |
Cy19 V165C R | ACCCGCATAgcaCCCAGAGACAAAGGGCGCCAC | 174 |
Cy19 V165S F | CCCTTTGTCTCTGGGAGTTATGCGGGTGATCCA | 175 |
Cy19 V165S R | TGGATCACCCGCATAACTCCCAGAGACAAAGGG | 176 |
Cy19 A167C F | GCGCCCTTTGTCTCTGGGGTTTATtgcGGT | 177 |
Cy19 A167C R | AGCACTCAGTTGTTGTGGATCACCGCAATA | 178 |
Cy19 A167L F | GTCTCTGGGGTTTATCTGGGTGATCCACAACAA | 179 |
Cy19 A167L R | TTGTTGTGGATCACCCAGATAAACCCCAGAGAC | 180 |
Cy19 A167I F | GTCTCTGGGGTTTATATCGGTGATCCACAACAA | 181 |
Cy19 A167I R | TTGTTGTGGATCACCGATATAAACCCCAGAGAC | 182 |
Cy19 V305L F | CATTCCCTATCCCACCCTAGCCTGTGTGGTCTTG | 183 |
Cy19 V305L R | CAAGACCACACAGGCTAGGGTGGGATAGGGAATG | 184 |
Cy19 V305M F | TATCCCACCatgGCCTGTGTGGTCTTGGCCTAT | 185 |
Cy19 V305M R | CACACAGGCCATGGTGGGATAGGGAATGGTGGCCAA | 186 |
Cy19 L327T F | AGTGTCCGCCCCGGCTTTGGCGTAaccATT | 187 |
Cy19 L327T R | GCGGATGCCCTGACTGCGAGGAATGGTTAC | 188 |
Cy19 F360I F | CAAGTCTTTACGAGTATTATTGGCGGTGCTAC | 189 |
Cy19 F360I R | GTAGCACCGCCAATAATACTCGTAAAGACTTG | 190 |
Cy19 F360L F | GTCTTTACGAGTTTAATTGGCGGTGCTACG | 191 |
Cy19 F360L R | CGTAGCACCGCCAATTAAACTCGTAAAGAC | 192 |
Cy19 F360M F | GGCAAGTCTTTACGAGTATGATTGGCGGTGCTACGG | 193 |
Cy19 F360M R | CCGTAGCACCGCCAATCATACTCGTAAAGACTTGCC | 194 |
Cy19 F360V F | CAAGTCTTTACGAGTGTTATTGGCGGTGCTAC | 195 |
Cy19 F360V R | GTAGCACCGCCAATAACACTCGTAAAGACTTG | 196 |
Cy19 F360T F | TTTACGAGTACTATTGGCGGTGCTACGGATCCTGA | 197 |
Cy19 F360T R | CCGCCAATAGTACTCGTAAAGACTTGCCAACCGGC | 198 |
Cy19 I408R F | GTTTGGCGACGGGCGAGACCCCAATATATGGTG | 199 |
Cy19 I408R R | CACCATATATTGGGGTCTCGCCCGTCGCCAAAC | 200 |
Cy19 I408W F | AGGTTTGGCGACGGGCGTGGCCCCAATATATG | 201 |
Cy19 I408W R | CATATATTGGGGCCACGCCCGTCGCCAAACCT | 202 |
Cy19 V165C+A167C F | CCTTTGTCTCTGGGTGTTATTGCGGTGATCCACAACAACTG | 203 |
Cy19 V165C+A167C R | CAGTTGTTGTGGATCACCGCAATAACACCCAGAGACAAAGG | 204 |
Cy19 V165C+A167L F | GGCGCCCTTTGTCTCTGGGTGCTACCTGGGTGATCCACAACA | 205 |
Cy19 V165C+A167L R | TGTTGTGGATCACCCAGGTAGCACCCAGAGACAAAGGGCGCC | 206 |
Cy19 V165S+A167C F | GGCGCCCTTTGTCTCTGGGAGCTACTGCGGTGATCCACAACAAC | 207 |
Cy19 V165S+A167C R | GTTGTTGTGGATCACCGCAGTAGCTCCCAGAGACAAAGGGCGCC | 208 |
Cy19 V165C+A167I F | GGCGCCCTTTGTCTCTGGGTGCTACATCGGTGATCCACA | 209 |
Cy19 V165C+A167I R | TGTGGATCACCGATGTAGCACCCAGAGACAAAGGGCGCC | 210 |
Cy19 V165S+A167I F | GGCGCCCTTTGTCTCTGGGAGCTACATCGGTGATCCACA | 211 |
Cy19 V165S+A167I R | TGTGGATCACCGATGTAGCTCCCAGAGACAAAGGGCGCC | 212 |
Cy19 V165S+A167L F | CCTTTGTCTCTGGGTCTTATCTGGGTGATCCACAACAACTG | 213 |
Cy19 V165S+A167L R | CAGTTGTTGTGGATCACCCAGATAAGACCCAGAGACAAAGG | 214 |
CyPPO20의 아미노산 변이 | 서열 (5'-> 3') | 서열번호 |
Cy20 R89A F | GATCGCCACCTACCGGCGTACATCTACTGGCGG | 215 |
Cy20 R89A R | CCGCCAGTAGATGTACGCCGGTAGGTGGCGATC | 216 |
Cy20 V165C F | CCCTTTGTCTCTGGGTGTTACGCCGGTGATCCG | 217 |
Cy20 V165C R | CGGATCACCGGCGTAACACCCAGAGACAAAGGG | 218 |
Cy20 V165S F | CCCTTTGTCTCTGGGAGTTACGCCGGTGATCCG | 219 |
Cy20 V165S R | CGGATCACCGGCGTAACTCCCAGAGACAAAGGG | 220 |
Cy20 A167C F | GTCTCTGGGGTTTACTGTGGTGATCCGCAACAA | 221 |
Cy20 A167C R | TTGTTGCGGATCACCACAGTAAACCCCAGAGAC | 222 |
Cy20 A167L F | GTCTCTGGGGTTTACCTCGGTGATCCGCAACAA | 223 |
Cy20 A167L R | TTGTTGCGGATCACCGAGGTAAACCCCAGAGAC | 224 |
Cy20 A167I F | GTCTCTGGGGTTTACATTGGTGATCCGCAACAA | 225 |
Cy20 A167I R | TTGTTGCGGATCACCAATGTAAACCCCAGAGAC | 226 |
Cy20 V305L F | CATCCCCTATCCCACCCTAGCCTGTGTGGTCTTG | 227 |
Cy20 V305L R | CAAGACCACACAGGCTAGGGTGGGATAGGGGATG | 228 |
Cy20 V305M F | ATCCCCTATCCCACCATGGCCTGTGTGGTCTTG | 229 |
Cy20 V305M R | CAAGACCACACAGGCCATGGTGGGATAGGGGAT | 230 |
Cy20 L327T F | CCCGGATTTGGCGTAACGATTCCTCGTGGCCAG | 231 |
Cy20 L327T R | CTGGCCACGAGGAATCGTTACGCCAAATCCGGG | 232 |
Cy20 I340T F | CGTACCCTTGGCACCACATGGTCGTCCTGTCTT | 233 |
Cy20 I340T R | AAGACAGGACGACCATGTGGTGCCAAGGGTACG | 234 |
Cy20 F360I F | CAAGTCTTTACAAGTACCATTGGCGGTGCCACG | 235 |
Cy20 F360I R | CGTGGCACCGCCAATGGTACTTGTAAAGACTTG | 236 |
Cy20 F360L F | CAAGTCTTTACAAGTCTGATTGGCGGTGCCACG | 237 |
Cy20 F360L R | CGTGGCACCGCCAATCAGACTTGTAAAGACTTG | 238 |
Cy20 F360M F | CAAGTCTTTACAAGTATGATTGGCGGTGCCACG | 239 |
Cy20 F360M R | CGTGGCACCGCCAATCATACTTGTAAAGACTTG | 240 |
Cy20 F360V F | CAAGTCTTTACAAGTGTTATTGGCGGTGCCACG | 241 |
Cy20 F360V R | CGTGGCACCGCCAATAACACTTGTAAAGACTTG | 242 |
Cy20 I408R F | GTTTGGCGACGGGCGAGGCCCCAATATCTTGTG | 243 |
Cy20 I408R R | CACAAGATATTGGGGCCTCGCCCGTCGCCAAAC | 244 |
Cy20 I408W F | GTTTGGCGACGGGCGTGGCCCCAATATCTTGTG | 245 |
Cy20 I408W R | CACAAGATATTGGGGCCACGCCCGTCGCCAAAC | 246 |
Cy20 V165C+A167C F | CCCTTTGTCTCTGGGTGTTACTGTGGTGATCCGCAACAA | 247 |
Cy20 V165C+A167C R | TTGTTGCGGATCACCACAGTAACACCCAGAGACAAAGGG | 248 |
Cy20 V165C+A167I F | CCCTTTGTCTCTGGGTGTTACATTGGTGATCCGCAACAA | 249 |
Cy20 V165C+A167I R | TTGTTGCGGATCACCAATGTAACACCCAGAGACAAAGGG | 250 |
Cy20 V165C+A167L F | CCCTTTGTCTCTGGGTGTTACCTCGGTGATCCGCAACAA | 251 |
Cy20 V165C+A167L R | TTGTTGCGGATCACCGAGGTAACACCCAGAGACAAAGGG | 252 |
Cy20 V165S+A167C F | CCCTTTGTCTCTGGGAGTTACTGTGGTGATCCGCAACAA | 253 |
Cy20 V165S+A167C R | TTGTTGCGGATCACCACAGTAACTCCCAGAGACAAAGGG | 254 |
Cy20 V165S+A167I F | CCCTTTGTCTCTGGGAGTTACATTGGTGATCCGCAACAA | 255 |
Cy20 V165S+A167I R | TTGTTGCGGATCACCAATGTAACTCCCAGAGACAAAGGG | 256 |
Cy20 V165S+A167L F | CCCTTTGTCTCTGGGAGTTACCTCGGTGATCCGCAACAA | 257 |
Cy20 V165S+A167L R | TTGTTGCGGATCACCGAGGTAACTCCCAGAGACAAAGGG | 258 |
CyPPO18의 아미노산 변이 | 서열 (5'-> 3') | 서열번호 |
Cy18 R89A F | GATCGCCACCTACCGGCATACATCTACTGGCG | 259 |
Cy18 R89A R | CGCCAGTAGATGTATGCCGGTAGGTGGCGATC | 260 |
Cy18 V165C F | CCCTTTGTCTCTGGGTGTTATGCCGGTGATC | 261 |
Cy18 V165C R | GATCACCGGCATAACACCCAGAGACAAAGGG | 262 |
Cy18 V165S F | CCCTTTGTCTCTGGGAGTTATGCCGGTGATC | 263 |
Cy18 V165S R | GATCACCGGCATAACTCCCAGAGACAAAGGG | 264 |
Cy18 A167C F | GTCTCTGGGGTTTATTGCGGTGATCCGCAAC | 265 |
Cy18 A167C R | GTTGCGGATCACCGCAATAAACCCCAGAGAC | 266 |
Cy18 A167L F | GTCTCTGGGGTTTATCTCGGTGATCCGCAAC | 267 |
Cy18 A167L R | GTTGCGGATCACCGAGATAAACCCCAGAGAC | 268 |
Cy18 A167I F | GTCTCTGGGGTTTATATCGGTGATCCGCAAC | 269 |
Cy18 A167I R | GTTGCGGATCACCGATATAAACCCCAGAGAC | 270 |
Cy18 V305L F | CATCCCCTATCCCACCCTAGCCTGTGTGGTGTTG | 271 |
Cy18 V305L R | CAACACCACACAGGCTAGGGTGGGATAGGGGATG | 272 |
Cy18 V305M F | CATCCCCTATCCCACCATGGCCTGTGTGGTGTTG | 273 |
Cy18 V305M R | CAACACCACACAGGCCATGGTGGGATAGGGGATG | 274 |
Cy18 L327T F | CCGGATTTGGAGTAACGGTTCCTCGTGGTC | 275 |
Cy18 L327T R | GACCACGAGGAACCGTTACTCCAAATCCGG | 276 |
Cy18 F360I F | CAAGTCTTCACCAGTATTATTGGCGGTGCTAC | 277 |
Cy18 F360I R | GTAGCACCGCCAATAATACTGGTGAAGACTTG | 278 |
Cy18 F360L F | GTCTTCACCAGTTTGATTGGCGGTGCTAC | 279 |
Cy18 F360L R | GTAGCACCGCCAATCAAACTGGTGAAGAC | 280 |
Cy18 F360M F | GCAAGTCTTCACCAGTATGATTGGCGGTGCTACGG | 281 |
Cy18 F360M R | CCGTAGCACCGCCAATCATACTGGTGAAGACTTGC | 282 |
Cy18 F360V F | CAAGTCTTCACCAGTGTTATTGGCGGTGCTAC | 283 |
Cy18 F360V R | GTAGCACCGCCAATAACACTGGTGAAGACTTG | 284 |
Cy18 V165C+A167L F | GCCCCCTTTGTCTCTGGGTGCTACCTCGGTGATCCGCAACAA | 285 |
Cy18 V165C+A167L R | TTGTTGCGGATCACCGAGGTAGCACCCAGAGACAAAGGGGGC | 286 |
Cy18 V165S+A167C F | GCCCCCTTTGTCTCTGGGAGCTACTGCGGTGATCCGCAACAA | 287 |
Cy18 V165S+A167C R | TTGTTGCGGATCACCGCAGTAGCTCCCAGAGACAAAGGGGGC | 288 |
Cy18 V165C+A167C F | CCCCCTTTGTCTCTGGGTGCTATTGCGGTGATCCGCA | 289 |
Cy18 V165C+A167C R | TGCGGATCACCGCAATAGCACCCAGAGACAAAGGGGG | 290 |
Cy18 V165C+A167I F | CCCCCTTTGTCTCTGGGTGCTACATCGGTGATCCGCA | 291 |
Cy18 V165C+A167I R | TGCGGATCACCGATGTAGCACCCAGAGACAAAGGGGG | 292 |
Cy18 V165S+A167I F | CCCCCTTTGTCTCTGGGAGCTACATCGGTGATCCGCA | 293 |
Cy18 V165S+A167I R | TGCGGATCACCGATGTAGCTCCCAGAGACAAAGGGGG | 294 |
Cy18 V165S+A167L F | GCCCCCTTTGTCTCTGGGAGCTACCTCGGTGATCCGCAACAA | 295 |
Cy18 V165S+A167L R | TTGTTGCGGATCACCGAGGTAGCTCCCAGAGACAAAGGGGGC | 296 |
실시예
3.
PPO
및 이의
변이체의
PPO
저해 제초제 내성 검증 (대장균에서 시험)
CyPPO19 및 CyPPO18의 PPO 저해 제초제 내성을 보다 높이기 위하여 각각 PPO 아미노산 서열 중 상기 실시예 2와 같은 아미노산 변이를 유발시킬 수 있는 PPO 유전자를 설계하여 PPO가 결핍된 BT3 대장균 (BT3(ΔPPO))에 형질전환한 후, PPO 저해 제초제를 처리한 환경에서 배양하여, 형질전환 대장균의 생장 저해 여부를 확인하였다.
구체적인 시험 과정은 다음과 같다:
상기 실시예 2에서 제작한 pET303-CyPPO19(wild type)와 pET303-CyPPO18(wild type) plasmid와 각각의 변이 유전자들(실시예 2 참조)이 포함된 plasmid를 BT3 competent cell에 열충격 방법으로 형질전환하여 앰피실린이 포함된 LB agar 배지에서 배양하였다.
각 유전자가 삽입된 BT3 형질전환체의 종배양을 위하여, 앰피실린이 포함된 LB broth (LPSS, 3 ml)에 단일 콜로니를 접종하여 12시간 이상 배양하고, 이 배양액 50~100 ㎕를 새로운 LB broth에 계대하여 흡광도 (OD600)가 0.5~1이 될 때까지 배양하고 이 배양액을 LB broth로 희석하여 흡광도를 0.5로 일정하게 맞춰준 후, LB broth로 10배씩 4번 희석하여 형질전환 대장균 배양 희석액을 준비하였다.
LB 25g/L, Bacto agar 12g/L, 앰피실린 (100 ㎍/ml), 및 다양한 제초제 stock을 농도별 (0~2,000 μM)로 혼합하여 제초제를 포함하는 배지를 준비하였다. 상기 제초제 stock은 모두 DMSO를 이용하여 제초제를 용해한 것이다.
상기 준비된 형질전환 대장균 배양 희석액을 제초제 배지에 10 ㎕씩 떨어뜨리고, 37 ℃ 및 암조건 하에서 16~20시간 동안 배양하고, 각 유전자를 포함한 형질전환 대장균들의 생장 정도를 육안으로 확인하여, 형질전환 대장균의 PPO 저해 제초제 내성을 시험하였다.
시험에 사용된 제초제를 아래의 표 8에 정리하였다:
Family | 제초제 |
Pyrimidinedione계 제초제 | Tiafenacil |
Saflufenacil | |
Diphenyl ether계 제초제 | Fomesafen |
Oxyfluorfen | |
N-phenylphthalimides계 제초제 | Flumioxazin |
Triazolinones계 제초제 | SulfentrazoneCarfentrazone |
상기 얻어진 제초제 내성을 CyPPO 야생형과 비교한 상대적 수준으로 평가하여 아래의 표 9 내지 표 10 및 도 3 내지 도 32에 나타내었다:
No. | CyPPO19 variants | Tiafenacil | Saflufenacil | Flumioxazin | Fomesafen | Sulfentrazone | Oxyfluorfen | Carfentrazone |
1 | R89A+F360M | ++ | ++++ | +++ | ++ | ++ | + | + |
2 | R89A+F360V | ++++ | ++++ | ++++ | + | +++ | + | + |
3 | R89A+F360I | +++ | ++++ | +++ | + | +++ | + | + |
4 | R89A+F360L | ++ | +++ | ++ | + | ++ | + | + |
5 | R89A+F360T | +++ | +++ | +++ | ++ | +++ | + | + |
6 | V165C+F360I | ++++ | ++++ | ++++ | + | ++++ | + | + |
7 | V165C+F360M | ++++ | ++++ | ++++ | +++ | ++++ | ++ | + |
8 | V165C+F360V | ++++ | ++++ | ++++ | + | ++++ | ++ | + |
9 | V165C+F360L | ++++ | ++++ | ++++ | +++ | ++++ | ++ | + |
10 | V165S+F360V | ++++ | ++++ | ++++ | + | ++++ | ++ | + |
11 | V165S+F360L | ++++ | ++++ | ++++ | ++ | +++ | ++ | + |
12 | V165S+F360T | ++++ | +++ | +++ | + | ++ | + | + |
13 | A167C+F360I | ++++ | +++ | ++++ | + | ++ | + | + |
14 | A167L+F360M | ++++ | ++++ | ++++ | ++ | ++ | + | + |
15 | A167I+F360L | +++ | +++ | +++ | + | + | + | + |
16 | A167L+F360T | +++ | +++ | +++ | + | ++ | + | + |
17 | A167C+F360M | ++++ | ++++ | ++++ | + | + | ++ | + |
18 | A167C+F360L | +++ | +++ | +++ | + | + | + | + |
19 | A167C+F360V | ++++ | +++ | +++ | + | + | + | + |
20 | V305M+F360I | ++++ | ++++ | ++++ | + | ++ | + | + |
21 | V305L+F360M | +++ | ++++ | ++++ | +++ | + | ++ | + |
22 | V305M+F360M | ++++ | ++++ | ++++ | +++ | ++ | ++ | + |
23 | V305M+F360V | ++++ | ++++ | ++++ | + | + | ++ | + |
24 | V305M+F360L | ++++ | ++++ | ++++ | ++ | ++ | ++ | + |
25 | V305L+F360L | ++++ | ++++ | ++++ | +++ | ++ | ++ | + |
26 | V305M+F360T | ++ | ++ | ++ | ++ | + | + | + |
27 | L327T+F360I | ++++ | ++ | ++++ | + | +++ | ++ | + |
28 | L327T+F360M | +++ | + | +++ | + | + | ++ | + |
29 | L327T+F360V | ++++ | ++ | ++++ | + | ++ | ++ | + |
30 | L327T+F360L | ++++ | + | ++++ | ++ | ++ | ++ | + |
31 | L327T+F360T | ++ | + | + | + | + | + | + |
32 | I408W+F360I | ++++ | +++ | +++ | +++ | +++ | ++ | + |
33 | I408R+F360M | +++ | ++ | ++ | + | + | + | + |
34 | I408W+F360V | ++++ | +++ | ++++ | +++ | +++ | ++ | + |
35 | I408R+F360L | ++ | + | + | + | + | + | + |
36 | I408W+F360T | ++ | ++ | + | + | + | + | + |
37 | R89A+V165C+F360I | ++++ | ++++ | ++++ | ++ | +++ | ++ | + |
38 | R89A+V165S+F360M | ++++ | ++++ | ++++ | +++ | +++ | +++ | + |
39 | R89A+V165C+F360V | ++++ | ++++ | ++++ | + | +++ | + | + |
40 | R89A+A167I+F360V | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
41 | R89A+A167C+F360L | ++++ | ++++ | ++++ | ++ | ++++ | +++ | ++ |
42 | R89A+A167L+F360T | ++++ | ++++ | ++++ | ++ | +++ | ++ | ++ |
43 | R89A+V305M+F360I | +++ | +++ | +++ | + | ++ | + | + |
44 | R89A+V305M+F360V | +++ | +++ | +++ | + | ++ | + | + |
45 | R89A+V305M+F360T | +++++ | +++++ | +++++ | +++ | +++++ | +++ | ++ |
46 | R89A+L327T+F360I | ++++ | ++ | ++++ | ++ | +++ | + | + |
47 | R89A+L327T+F360M | +++ | ++ | ++++ | +++ | ++ | ++ | + |
48 | R89A+L327T+F360T | +++++ | +++++ | +++++ | +++++ | +++++ | +++ | + |
49 | R89A+I408R+F360M | +++ | ++++ | ++++ | +++ | +++ | ++ | + |
50 | R89A+I408W+F360V | +++++ | +++++ | +++++ | +++++ | +++++ | +++ | + |
51 | R89A+I408R+F360L | +++ | +++ | +++ | + | ++ | + | + |
52 | V165S+A167I+F360M | +++++ | ++++ | +++++ | ++ | +++ | ++ | + |
53 | V165S+A167C+F360L | ++++ | +++ | ++++ | + | ++ | + | + |
54 | V165C+A167L+F360T | ++++ | +++ | +++ | ++ | +++ | + | + |
55 | V165C+V305M+F360I | +++++ | +++++ | +++++ | ++ | +++ | ++ | + |
56 | V165C+V305M+F360V | +++++ | +++++ | +++++ | ++ | ++++ | +++ | + |
57 | V165S+V305L+F360L | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
58 | V165C+L327T+F360I | +++++ | ++++ | +++++ | ++ | +++++ | +++ | + |
59 | V165C+L327T+F360V | +++++ | ++++ | +++++ | + | +++++ | +++ | + |
60 | V165S+L327T+F360T | ++++ | ++++ | +++++ | +++ | +++ | ++ | + |
61 | V165C+I408W+F360V | ++++ | ++++ | ++++ | +++ | +++ | ++++ | + |
62 | V165C+I408W+F360T | ++++ | ++++ | ++++ | + | +++ | ++ | + |
63 | A167I+V305M+F360L | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
64 | A167C+V305L+F360V | ++++ | +++++ | +++++ | + | + | ++ | + |
65 | A167L+V305M+F360T | ++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
66 | A167C+L327T+F360I | ++++ | +++ | ++++ | ++ | ++++ | +++ | + |
67 | A167L+L327T+F360M | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
68 | A167I+L327T+F360V | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
69 | A167C+I408W+F360I | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
70 | A167I+I408W+F360V | ++++ | ++++ | ++++ | + | +++ | ++ | + |
71 | A167L+I408R+F360L | ++++ | ++++ | ++++ | +++ | ++++ | +++ | + |
72 | V305M+L327T+F360I | ++++ | ++ | ++++ | + | ++ | ++ | + |
73 | V305L+L327T+F360V | +++++ | +++ | +++++ | + | ++ | +++ | + |
74 | V305M+L327T+F360T | +++ | +++ | +++ | ++ | +++ | ++ | + |
75 | V305M+I408W+F360I | +++++ | +++++ | +++++ | +++ | +++ | +++ | + |
76 | V305M+I408W+F360V | +++++ | +++++ | +++++ | +++ | ++++ | +++ | + |
77 | V305L+I408R+F360L | +++++ | +++++ | ++++ | + | ++ | ++ | + |
78 | L327T+I408R+F360M | +++++ | +++ | +++++ | ++ | + | +++ | + |
79 | L327T+I408W+F360V | +++++ | +++++ | +++++ | +++++ | ++++ | +++++ | ++ |
80 | R89A+V165C+A167C+F360I | +++ | +++ | +++ | ++ | ++ | + | + |
81 | R89A+V165S+V305M+F360M | +++++ | +++++ | +++++ | +++ | +++ | ++ | + |
82 | R89A+V165C+L327T+F360V | +++ | +++ | ++++ | + | ++ | + | + |
83 | R89A+V165S+I408R+F360L | ++++ | ++++ | ++++ | ++ | +++ | ++ | + |
84 | R89A+A167L+V305L+F360T | +++ | +++ | ++ | ++ | ++ | ++ | + |
85 | R89A+A167L+L327T+F360I | ++++ | ++++ | ++++ | +++ | ++++ | +++++ | ++ |
86 | R89A+A167C+I408R+F360M | +++ | +++ | ++++ | ++ | ++ | ++ | + |
87 | V165C+A167I+V305M+F360V | ++ | ++ | ++ | + | + | + | + |
88 | V165S+A167C+L327T+F360M | ++++ | ++++ | ++++ | ++ | ++++ | ++ | + |
89 | V165C+A167C+I408W+F360T | ++++ | ++++ | ++++ | ++ | +++ | ++ | + |
90 | A167I+V305L+L327T+F360V | +++++ | ++++ | +++++ | +++ | ++++ | +++ | + |
91 | A167L+V305M+I408R+F360M | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
92 | V305L+L327T+I408W+F360V | +++++ | +++++ | +++++ | +++ | +++++ | +++ | + |
No. | CyPPO18 variants | Tiafenacil | Saflufenacil | Flumioxazin | Fomesafen | Sulfentrazone | Oxyfluorfen | Carfentrazone |
1 | R89A+F360V | ++++ | ++++ | ++++ | +++ | ++++ | + | ++ |
2 | A167L+F360I | ++++ | ++++ | ++++ | +++ | ++++ | + | ++ |
3 | V305M+F360I | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++ |
4 | L327T+F360M | ++++ | +++ | ++++ | ++++ | ++++ | ++ | ++ |
5 | R89A+V165C+F360I | +++++ | +++++ | +++++ | ++++ | +++++ | ++ | ++ |
6 | V165C+A167L+F360I | +++++ | +++++ | +++++ | ++++ | +++++ | ++ | ++ |
7 | A167L+V305M+F360M | +++++ | +++++ | +++++ | +++++ | +++++ | ++ | ++ |
8 | V165S+A167L+V305M+F360I | +++++ | +++++ | +++++ | +++++ | +++++ | ++ | ++ |
9 | R89A+V165S+V305M+F360I | +++++ | +++++ | +++++ | +++++ | +++++ | ++ | ++ |
10 | R89A+V165C+A167L+V305M+F360I | +++++ | +++++ | +++++ | +++++ | +++++ | ++ | ++ |
상기 표 9 내지 10에서, 야생형의 제초제 내성 수준과 동등 수준의 내성을 "-"로, 10배 높을수록“+”를 덧붙여 최대 “+++++”까지 등급을 정하여 평가하였다.
도 3 내지 도 30 (CyPPO19의 야생형 또는 변이형) 및 도 31 및 도 32 (CyPPO18의 야생형 또는 변이형)은 CyPPO 유전자 (야생형 또는 변이형)가 형질도입된 대장균의 배양 결과를 보여주는 사진으로, 상단에 기재된 농도는 제초제 처리 농도이며, 각 농도별 5개의 컬럼은 대장균 배양액을 10배씩 희석한 결과를 나타낸 것으로, 가장 왼쪽 컬럼이 OD600=0.5인 대장균 배양액의 실험 결과이고, 가장 오른쪽 컬럼이 OD600=0.00005인 대장균 배양액의 실험 결과이다.
상기 표 9 내지 10 및 도 3 내지 도 32에서 보여지는 바와 같이, CyPPO19 및 CyPPO18의 변이 유전자가 도입된 형질전환체는 모두 다양한 종류의 제초제에 대하여 야생형 유전자가 도입된 형질전환체보다 현저하게 상승된 수준 (적어도 10배 이상)의 제초제 내성을 나타냄을 확인할 수 있다.
실시예 4: PPO의 효소 활성 및 제초제별 IC
50
값 측정
PPO 단백질 및 특정 위치의 아미노산을 변이시킨 PPO 단백질 변이체의 효소 활성을 측정하고 PPO저해 제초제에 의한 inhibition assay를 수행하였다. PPO 단백질은 수용성이 낮지만, MBP (maltose binding protein)와 함께 fusion protein (MBP-PPO)으로 발현시킬 경우 안정적으로 수용성 단백질이 발현되는 것을 확인하여, 하기의 야생형 및 변이형 단백질을 MBP와의 융합 단백질 형태로 발현시켜서 본 시험에 사용하였다 (도 33).
CyPPO19 및 CyPPO18의 야생형 유전자 및 변이 유전자 (실시예 1 및 실시예 2 참조)를 발현시키기 위하여, 상기 유전자를 각각 pMAL-c2X 벡터 (도 1 참조)에 삽입한 후, BL21 CodonPlus (DE3) 대장균에 각각 형질전환하였다.
상기 얻어진 형질전환 대장균을 다음의 조건으로 배양하여 삽입된 PPO 유전자를 발현시켰다:
Induction: OD600=0.2, 최종농도 0.3 mM IPTG 첨가;
발현온도: 23 ℃, 200 rpm의 진탕 배양;
발현시간: 16 hrs
배양규모: 200 ml/1,000 ml flask.
상기 배양된 형질전환 대장균에 대하여 다음의 과정으로 세포 파쇄 및 단백질 추출을 수행하였다:
추출 버퍼: Column buffer (50 mM Tris-Cl, pH8.0, 200 mM NaCl) 5 ml buffer/g cell;
Sonication: SONICS&MATERIALS社 VCX130 (130 watts);
15 sec ON, 10 sec OFF for 5 min on ice;
4 ℃ 및 20분 조건 하에서 원심분리 (20,000 xg); 및
상기 원심분리로 얻어진 상층액을 column buffer를 사용하여 1:6 비율로 희석하였다.
상기 얻어진 단백질 추출물에 대하여 다음의 과정을 4 ℃ 저온실에서 수행하여 PPO 단백질의 정제를 수행하였다. 1.5x15 cm column (Bio-Rad Econo Columns 1.5 x 10 cm, glass chromatography column, max. vol)에 amylose resin (New England Biolabs)을 부어 컬럼을 packing하고, 상기 얻어진 단백질 추출물을 컬럼에 0.2 ml/min의 속도로 로딩하였다. 상기 컬럼을 컬럼 부피 기준으로 3부피배의 column buffer로 washing 후 washing액 내의 단백질 양을 확인하여 더 이상 단백질이 검출되지 않으면 washing을 종료하였다. 상기 컬럼 부피 기준으로 약 2부피배의 20 mM maltose 를 포함하는 column buffer로 MBP-PPO 단백질을 elution (1.5 ml E-tube에 각 fraction을 분취)하면서 각 eluent의 단백질 농도를 확인하였다. 더 이상 단백질이 검출되지 않으면 elution을 종료시켰다. 각 fraction을 10 ㎕의 양으로 취하여 단백질 정량 후 단백질이 검출된 fraction을 SDS-PAGE로 분석하고, 순도가 높은 fraction을 취하여 효소 활성 분석 시험에 사용하였다.
상기 정제된 CyPPO19 및 CyPPO18의 야생형 단백질 및 변이형 단백질의 효소 활성을 아래의 과정으로 측정하였다.
우선, PPO 단백질의 기질인 프로토포르피리노겐 IX (Protoporphyrinogen IX)를 합성하였다. 이 과정은 질소기체가 streaming 되는 공간에서 수행되었다. 프로토포르피린 IX (protoporphyrin IX) 6 mg을 20% (v/v) EtOH 20 ml에 용해시키고, dark condition에서 30분간 교반하였다. 상기 얻어진 프로토포르피린 IX 용액을 15 ml screw tube에 1,000 ㎕의 양으로 넣고 5분간 질소 기체를 flushing하였다. 여기에 아말감나트륨 (sodium amalgam) 1g을 넣고 2분간 vigorous shaking하였다. Tube 안의 수소 기체를 배출하기 위해 뚜껑을 열어두었다. 그 후, 뚜껑을 닫고 3분간 인큐베이션한 후, Syringe와 cellulose membrane filter를 이용해 프로토포르피리노겐 IX 용액을 filtering하였다. 상기 얻어진 프로토포르피리노겐 IX 용액 800 ㎕에 2M MOPS [3-(N-morpholino)propanesulfonic acid]를 약 1,600 ㎕의 양으로 첨가하여 pH를 7.5으로 조절하였다. PPO 단백질의 효소 활성을 측정하기 위하여 다음의 조성으로 Reaction mixture를 준비하였다 (10 ml 기준): 50 mM Tris-Cl (pH 7.5); 50 mM NaCl; 0.04% (v/v) Tween 20; 40 mM glucose (0.072g); 5 units glucose oxidase (16.6 mg); 및 10 units catalase (1 ㎕).
상기 Reaction mixture 180 ㎕를 96 well plates에 넣고 앞서 정제된 PPO 단백질 (상기 MBP-fusion 단백질을 정제한 산물) 20 ㎕를 첨가하였으며, 약 50 ㎕의 mineral oil로 표면을 커버하였다. 기질인 프로토포르피리노겐 IX 용액을 최종농도 50 μM이 되도록 첨가하고 실온에서 30분간 반응시켰다. Microplate reader (Hidex社 sense)를 이용하여 프로토포르피린 IX (Protoporphyrin IX)의 형광을 측정하였다 (excitation: 405 nm; emission: 633 nm). PPO 효소 활성값을 계산하기 위해, 상기 프로토포르피리노겐 IX 용액이 들어있는 튜브를 공기 중에 개방하여 용액을 12 시간 이상 산화시켰다. 여기에 2.7N HCl를 첨가하고 408nm에서의 흡광도(absorbance)를 측정하였다. Standard 프로토포르피린 IX을 이용해 standard curve를 작성하고 이를 이용해 프로토포르피리노겐 IX의 농도를 calibration 하여 PPO 활성을 측정하였다.
PPO 효소 활성을 50% 저해하는 농도 (IC50)를 각 제초제별로 측정하였다. 이 때, 각 제초제의 최종 농도는 아래와 같이 하였다:
- Tiafenacil, saflufenacil, flumioxazin, fomesafen, , sulfentrazone, Oxyfluorfen, 및 Carfentrazone 각 제초제의 최종농도: 0, 10, 50, 100, 250, 500, 1,000, 2,500, 5,000, 10,000 nM
IC50값은 상기한 효소 활성 측정 과정에 제초제를 상기 농도로 첨가하여 PPO 효소 활성을 제초제 첨가 전의 50%로 저해하는 제초제의 농도로 구하였다.
이와 같이 얻어진 제초제 별 IC50을 아래의 표 11 및 표 12에 나타내었다.
CyPPO19 WT 및 variants | IC50 (nM) | |||||||
Tiafenacil | Saflufenacil | Flumioxazin | Fomesafen | Sulfentrazone | Oxyfluorfen | Carfentrazone | ||
WT | 67 | 158 | 55 | 242 | 516 | 570 | 763 | |
1 | R89A | 113 | 616 | 163 | 2,364 | 1,384 | 602 | 857 |
2 | V165C | 186 | 280 | 159 | 330 | 2,545 | 3,215 | 3,094 |
3 | V165S | 269 | 277 | 225 | 348 | 1,213 | 1,667 | 10,000 |
4 | A167C | 342 | 395 | 197 | 843 | 2,240 | 649 | 3,527 |
5 | A167I | 248 | 799 | 154 | 899 | 3,521 | 1,331 | 10,000 |
6 | A167L | 597 | 10,000 | 10,000 | 1,755 | 10,000 | 5,261 | 10,000 |
7 | V305M | 392 | 435 | 437 | 257 | 693 | 732 | 910 |
8 | V305L | 1,135 | 1,132 | 367 | 466 | 806 | 793 | 1,329 |
9 | L327T | 2,614 | 761 | 2,599 | 1,035 | 10,000 | 8,612 | 10,000 |
10 | F360M | 1,328 | 328 | 1,198 | 584 | 10,000 | 4,895 | 2,507 |
11 | F360I | 4,863 | 1,109 | 10,000 | 492 | 10,000 | 928 | 10,000 |
12 | F360L | 4,385 | 10,000 | 3,865 | 851 | 10,000 | 754 | 10,000 |
13 | F360V | 4,791 | 10,000 | 10,000 | 391 | 10,000 | 638 | 10,000 |
14 | F360T | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
15 | I408R | 1,039 | 10,000 | 696 | 2,097 | 10,000 | 10,000 | 10,000 |
16 | I408W | 3,001 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
17 | R89A+F360M | 3,751 | 10,000 | 2,090 | 10,000 | 10,000 | 5,291 | 6,192 |
18 | V165C+F360M | 10,000 | 10,000 | 7,763 | 10,000 | 10,000 | 5,266 | 10,000 |
19 | V165S+F360V | 10,000 | 10,000 | 10,000 | 936 | 10,000 | 2,070 | 10,000 |
20 | A167C+F360I | 10,000 | 10,000 | 10,000 | 871 | 10,000 | 6,011 | 10,000 |
21 | A167I+F360L | 10,000 | 10,000 | 10,000 | 1,248 | 10,000 | 10,000 | 10,000 |
22 | A167L+F360M | 10,000 | 10,000 | 10,000 | 3,151 | 10,000 | 10,000 | 10,000 |
23 | V305M+F360M | 10,000 | 10,000 | 3,104 | 1,077 | 10,000 | 5,044 | 10,000 |
24 | L327T+F360M | 10,000 | 1,618 | 10,000 | 2,266 | 10,000 | 10,000 | 10,000 |
25 | V165C+A167C+F360I | 10,000 | 10,000 | 10,000 | 988 | 10,000 | 10,000 | 10,000 |
26 | V165S+A167L+F360M | 10,000 | 10,000 | 10,000 | 7,003 | 10,000 | 10,000 | 10,000 |
27 | R89A+L327T+F360M | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
28 | V165C+L327T +F360I | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
29 | A167I+L327T+F360V | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
30 | V305L+L327T+F360M | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
31 | L327T+I408W+F360V | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |
CyPPO18 WT 및 variants | IC50 (nM) | |||||||
Tiafenacil | Saflufenacil | Flumioxazin | Fomesafen | Sulfentrazone | Oxyfluorfen | Carfentrazone | ||
WT | 18 | 32 | 13 | 629 | 27 | 103 | 566 | |
1 | R89A | 98 | 1,231 | 106 | 2,853 | 1,566 | 491 | 872 |
2 | V165C | 95 | 182 | 29 | 1,739 | 104 | 502 | 1,217 |
3 | V165S | 119 | 89 | 62 | 1,305 | 185 | 1,487 | 2,229 |
4 | A167C | 94 | 435 | 65 | 1,209 | 762 | 570 | 1,882 |
5 | A167L | 813 | 1,933 | 1,288 | 2,681 | 649 | 2,812 | 4,015 |
6 | A167I | 672 | 1,326 | 231 | 1,377 | 792 | 2,004 | 2,602 |
7 | V305M | 177 | 132 | 107 | 1,251 | 254 | 355 | 1,613 |
8 | L327T | 233 | 89 | 108 | 1,789 | 256 | 3,599 | 723 |
9 | F360M | 130 | 1,618 | 99 | 1,646 | 118 | 329 | 2,181 |
10 | F360I | 822 | 4,093 | 3,287 | 3,711 | 203 | 455 | 3,928 |
11 | F360L | 195 | 5,000 | 1,188 | 2,712 | 356 | 538 | 2,512 |
12 | F360V | 336 | 5,000 | 882 | 1,934 | 175 | 299 | 4,237 |
13 | R89A+F360M | 1,002 | 5,000 | 1,288 | 5,000 | 5,000 | 5,000 | 5,000 |
14 | A167L+F360M | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 |
15 | L327T+F360M | 5,000 | 1,662 | 3,841 | 5,000 | 855 | 5,000 | 5,000 |
16 | V165C+A167L+F360M | 5,000 | 5,000 | 5,000 | 5,000 | 4,483 | 5,000 | 5,000 |
17 | V165S+A167C+L327T+F360M | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 |
18 | R89A+V165S+A167C+L327T+F360M | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 | 5,000 |
(상기 표 11 및 12에서, 'IC50 5,000' 또는 'IC50 10,000'으로 표기된 것은 각 제초제의 해당농도(5,000 nM, 또는 10,000 nM)에서도 효소 활성이 50%로 저해되지 않아, 실제 IC50 값은 5,000 또는 10,000과 같거나 더 높음을 의미함)
상기 표 11 및 표 12에서 확인되는 바와 같이, CyPPO 단백질 변이체의 경우 야생형과 비교하여 각 제초제의 IC50값이 현저하게 상승하는 것을 확인할 수 있다. 이러한 결과는 PPO 단백질의 특정 위치의 아미노산 변이에 의하여 제초제에 대한 내성이 증가됨을 입증하는 것이다. 본 시험 결과에서 CyPPO 단백질 변이체가 대체적으로 야생형에 비하여 감소된 효소활성을 갖는 것으로 나타났는데, 이는 재조합 단백질과 식물에서 정제된 단백질간의 단백질 접힘 또는 소수성이 다른 데서 기인하는 것으로 생각된다. 식물에 있는 PPO 단백질은 식물체의 엽록체 막에 존재하며, 소수성이지만, 대장균이 생산하는 재조합 PPO 단백질은 MBP 단백질과 융합단백질로 친수성이다. 따라서, 변이 PPO 단백질이 식물의 엽록체에서 정상적인 조건으로 발현되면, 변이 단백질과 야생형 단백질간의 효소 활성 차이가 크지 않을 것으로 생각된다.
실시예 5: 다양한 남세균, 조류, 또는 세균 유래 PPO 단백질 변이체
상기 실시예 3 및 4에서 효과가 입증된 CyPPO19 및 CyPPO18의 변이 위치를 기초로, 다양한 남세균, 조류, 또는 세균 유래 PPO 단백질의 3차원 구조 분석을 통하여, CyPPO19 및 CyPPO18의 변이체와 유사한 기능을 가질 것으로 예측되는 변이체들의 변이위치 (mutated sites)를 아래의 표 13에 정리하였다:
서열번호 | 변이위치 | 1 | 2 | 3 | 4 | 5 | 6 | Accession No. |
변이위치의 변이(치환) 후 아미노산 | A | C, 또는S | C, I, 또는L | M, 또는L | T | M, I, L, T, 또는V | ||
1 | Thermosynechococcus elongatus PKUAC-SCTE542 | R89 | V165 | A167 | V305 | L327 | F360 | CP032152.1 |
2 | Cyanobacteria bacterium J003 | R89 | V165 | A167 | V305 | L327 | F360 | RMH63851.1 |
3 | Thermosynechococcus vulcanus NIES-2134 | R89 | V165 | A167 | V305 | L327 | F360 | BAY51976.1 |
4 | Synechococcus lividus | R92 | V168 | A170 | V312 | L334 | F367 | WP_099798264.1 |
5 | Microcoleaceae bacterium UBA10368 | R93 | V174 | A176 | V319 | L341 | Y374 | HBK97224.1 |
6 | Microcoleaceae bacterium UBA11344 | R87 | V168 | A170 | V313 | L335 | Y368 | HAT14741.1 |
7 | Oscillatoriales cyanobacterium (TAE85894.1) | R92 | V173 | A175 | V327 | L349 | Y382 | TAE85894.1 |
8 | Cyanobacteria bacterium UBA8156 | R92 | V166 | A168 | V309 | L331 | F364 | HAN47127.1 |
9 | Rubidibacter lacunae | R86 | V160 | A162 | V309 | L331 | F364 | WP_022605844.1 |
10 | Hydrocoleum sp. CS-953 | R92 | V171 | A173 | V317 | L339 | F372 | WP_094676324.1 |
11 | Oscillatoriales cyanobacterium (TAE55813.1) | R92 | V173 | A175 | V327 | L349 | Y382 | TAE55813.1 |
12 | Crinalium epipsammum | R85 | V166 | A168 | V312 | L334 | F367 | WP_015202233.1 |
13 | Oscillatoriales cyanobacterium (TAE70643.1) | R92 | V173 | A175 | V327 | L349 | Y382 | TAE70643.1 |
14 | Oscillatoriales cyanobacterium (TAE14532.1) | R92 | V173 | A175 | V327 | L349 | Y382 | TAE14532.1 |
15 | Cyanobacteria bacterium QS_8_64_29 | R85 | V166 | A168 | V312 | L334 | F367 | PSP16006.1 |
16 | Lyngbya aestuarii | R93 | V174 | A176 | V320 | L342 | Y375 | WP_023067908.1 |
17 | Tychonema bourrellyi | R93 | V174 | A176 | V319 | L341 | F374 | WP_096831359.1 |
18 | Oscillatoriales cyanobacterium (TAG91209.1) | R93 | V174 | A176 | V319 | L341 | Y374 | TAG91209.1 |
19 | Cyanobacteria bacterium SW_9_44_58 | R85 | V160 | A162 | V308 | L330 | F363 | PSO49761.1 |
20 | Trichodesmium erythraeum | R92 | V173 | A175 | V319 | L341 | Y374 | WP_011611816.1 |
21 | Geitlerinema sp. PCC 9228 | R105 | V180 | A182 | V332 | L355 | F388 | WP_071516524.1 |
22 | Oscillatoriales cyanobacterium (TAD79992.1) | R104 | V185 | A187 | V334 | L356 | F389 | TAD79992.1 |
23 | Oscillatoriales cyanobacterium (TAD82603.1) | R93 | V174 | A176 | V319 | L341 | Y374 | TAD82603.1 |
24 | Oscillatoriales cyanobacterium (TAD95528.1) | R93 | V174 | A176 | V319 | L341 | Y374 | TAD95528.1 |
25 | Limnothrix sp. PR1529 | R88 | V169 | A171 | V318 | L340 | F373 | WP_099534595.1 |
26 | Planktothricoides sp. SR001 | R102 | V183 | A185 | V329 | L352 | F385 | WP_054468037.1 |
27 | Limnothrix sp. CACIAM 69d | R88 | V169 | A171 | V318 | L340 | F373 | RFP59749.1 |
28 | Okeania hirsuta (WP_124155207.1) | R92 | V172 | A174 | V318 | L340 | F373 | WP_124155207.1 |
29 | Okeania hirsuta (WP_124145785.1) | R92 | V172 | A174 | V318 | L340 | F373 | WP_124145785.1 |
30 | Desertifilum sp. IPPAS B-1220 | R96 | V177 | A179 | V324 | L346 | Y379 | WP_069967861.1 |
31 | Synechococcus sp. 65AY6Li | R105 | V179 | A181 | V322 | L344 | F377 | PIK93057.1 |
32 | Synechococcus sp. 65AY6A5 | R105 | V179 | A181 | V322 | L344 | F377 | PIK88626.1 |
33 | Synechococcus sp. 60AY4M2 | R105 | V179 | A181 | V322 | L344 | F377 | PIK94415.1 |
34 | Synechococcus sp. 63AY4M1 | R105 | V179 | A181 | V322 | L344 | F377 | PIK96673.1 |
35 | Synechococcus sp. 63AY4M2 | R105 | V179 | A181 | V322 | L344 | F377 | PIK85371.1 |
36 | Cyanobacteria bacterium J007 | R100 | V177 | A179 | V323 | L345 | F378 | RMH78328.1 |
37 | Spirulina major | R85 | V166 | A168 | V311 | L333 | F366 | WP_072619201.1 |
38 | Euhalothece sp. KZN 001 | R85 | V160 | A162 | V306 | L328 | F361 | PNW65677.1 |
39 | Dactylococcopsis salina | R85 | V160 | A162 | V306 | L328 | F361 | WP_015230904.1 |
40 | Synechococcus sp. PCC 7336 | R89 | V163 | A165 | V306 | L328 | Y361 | WP_017328280.1 |
41 | Arthrospira sp. O9.13F | R93 | V174 | A176 | V320 | L342 | Y375 | WP_111891435.1 |
42 | Arthrospira platensis (WP_006622155.1) | R93 | V174 | A176 | V320 | L342 | Y375 | WP_006622155.1 |
43 | Arthrospira platensis (WP_006617829.1) | R93 | V174 | A176 | V321 | L343 | Y376 | WP_006617829.1 |
44 | Pseudanabaena sp. BC1403 | R98 | V172 | A174 | V318 | L340 | F373 | WP_103669271.1 |
45 | Pseudanabaena sp. 'Roaring Creek' | R97 | V171 | A173 | V316 | L338 | F371 | WP_055076288.1 |
46 | Pseudanabaena sp. (HBC40803.1) | R98 | V172 | A174 | V319 | L341 | F374 | HBC40803.1 |
47 | Synechococcus sp. JA-2-3B'a(2-13) | R106 | V180 | A182 | V343 | L365 | F398 | WP_049749573.1 |
48 | Pseudanabaena biceps | R97 | V171 | A173 | V317 | L339 | F372 | WP_009629673.1 |
49 | Pseudanabaena sp. (PZV12410.1) | R98 | V172 | A174 | V318 | L340 | F373 | PZV12410.1 |
50 | Pseudanabaena sp. PCC 7367 | R104 | V178 | A180 | V336 | L358 | F391 | WP_015165508.1 |
51 | Pseudanabaena sp. SR411 | R98 | V172 | A174 | V319 | L341 | F374 | WP_094530677.1 |
52 | Pseudanabaena frigida | R98 | V172 | A174 | V318 | L340 | F373 | PZO41121.1 |
53 | Pseudanabaena sp. (PZU98053) | R99 | V173 | A175 | V320 | L342 | F375 | PZU98053 |
54 | Oscillatoriales cyanobacterium CG2_30_44_21 | R106 | V180 | A182 | V327 | L349 | F382 | OIP76421.1 |
55 | Chlamydomonas reinhardtii | R167 | V241 | A243 | V389 | L418 | Y451 | AF068635.1 |
56 | Volvox carteri f. nagariensis | R168 | V242 | A244 | V389 | L418 | Y451 | XM_002955148.1 |
57 | Chondrus crispus (CHC_T00000813001) | R106 | V181 | A183 | V328 | L350 | Y383 | XM_005718155.1 |
58 | Galdieria sulphuraria | R167 | V242 | A244 | V399 | L421 | Y454 | XM_005708373.1 |
59 | Pseudanabaena sp. ABRG5-3 | R98 | V172 | A174 | V319 | L341 | F374 | WP_126386150.1 |
60 | Arthrospira platensis YZ | R93 | V174 | A176 | V321 | L343 | Y376 | WP_014277531.1 |
61 | Gloeobacter kilaueensis JS1 | R94 | V167 | A169 | V301 | L323 | F356 | WP_023175091 |
62 | Gloeobacter violaceus PCC 7421 | R91 | M164 | A166 | V298 | L320 | F353 | WP_011140945 |
63 | Panicum hallii var. hallii | R145 | V219 | A221 | V366 | L394 | Y427 | PUZ57154.1 |
64 | Porphyra umbilicalis | R157 | V232 | A234 | V381 | L403 | Y436 | OSX75961.1 |
65 | Ostreococcus tauri | R124 | V198 | A200 | V349 | L380 | Y415 | XP_003079975.1 |
66 | Ectocarpus siliculosus | R144 | V220 | A222 | V374 | L396 | Y429 | CBJ31610.1 |
67 | Nannochloropsis gaditana CCMP526 | R87 | V162 | A164 | V311 | L333 | Y365 | XM_005854685.1 |
68 | Ostreococcus lucimarinus CCE9901 | R67 | V141 | A143 | V288 | L319 | Y354 | XM_001418241.1 |
69 | Guillardia theta CCMP2712 | R159 | V233 | A235 | V381 | L414 | Y447 | XM_005821253.1 |
70 | Cyanidioschyzon merolae strain 10D | R168 | V259 | A261 | V413 | L435 | Y468 | XM_005535077.1 |
71 | Bathycoccus prasinos | R152 | V227 | A229 | V378 | L409 | Y444 | XM_007513261.1 |
72 | Myxococcus Xanthus Synthetic construct | R94 | I168 | A170 | I311 | L332 | M365 | AY916795.1 |
73 | Myxococcus virescens | R94 | I168 | A170 | I310 | L331 | M364 | WP_090484749.1 |
74 | Myxococcus macrosporus DSM 14697 | R86 | I159 | A161 | I304 | L325 | M358 | ATB45699.1 |
75 | Myxococcus hansupus | R94 | I168 | A170 | I310 | L331 | M364 | WP_044889345.1 |
76 | Myxococcus fulvus | R85 | I159 | A161 | I305 | L326 | M359 | WP_046711394.1 |
77 | Myxococcus fulvus | R85 | I159 | A161 | I305 | L326 | M359 | WP_074949681.1 |
78 | Myxococcus stipitatus | R85 | I159 | A161 | I303 | L324 | M357 | WP_015346914.1 |
79 | Hyalangium minutum | A86 | I160 | A162 | I305 | L326 | M359 | WP_044193071.1 |
실시예
6.
CyPPO
야생형 및
변이체를
이용한 애기장대 형질전환체 제작 및 제초제 내성 실험
6-1. 애기장대 형질전환 벡터 제작 및 애기장대 형질전환체 제작
애기장대 형질전환은 선별 마커인 Bar 유전자 (glufosinate 내성 유전자)의 ORF와 각각의 CyPPO19 또는 CyPPO18의 아미노산 변이체 유전자의 ORF를 가진 이원벡터 (binary vector)를 제작하여 수행하였다. 또한, Bar 유전자는 PPO 저해 제초제와 작용 기전이 다른 제초제를 교차 사용하는 경우 그 효과를 확인하기 위하여 사용하였으며, 형질전환한 유전자가 후대에 안정적으로 유전되고 있는지 여부를 검증하는 데에도 사용하였다. Bar 유전자의 발현을 위하여 NOS 프로모터와 전사 종결을 위한 E9 터미네이터를 사용하였다.
CyPPO19, CyPPO19 변이체, CyPPO18, 및 CyPPO18 변이체 각각을 식물체에서 발현시키기 위하여 CaMV35S 프로모터와 NOS 터미네이터를 사용하였다.
CyPPO19, CyPPO19 변이체, CyPPO18, 및 CyPPO18 변이체 유전자는 표 14 프라이머를 이용하여 증폭한 후 XhoI, BamHI 제한효소를 이용하여 상기 binary vector에 삽입하였다.
프라이머 | 서열 (5'→ 3') | 서열번호 |
CyPPO19_XhoI_F | CTCGAGATGTCTGAGGTGGACGTTGCC | 297 |
CyPPO19_BamHI_R | GGATCCAGGTTGGCCCCCGGAAAGATA | 298 |
CyPPO18_XhoI_F | CTCGAGATGATTGAAGTGGATGTGGCT | 299 |
CyPPO18_BamHI_R | GGATCCTGATTGTCCACCAGCGAG | 300 |
또한, 발현된 단백질의 확인을 위해 헤마글루티닌 (hemagglutinin, HA) tag을 BamHI, SacI 제한효소를 이용하여 PPO 유전자의 3' 말단 부위에 삽입하였다. HA tag 뒤에 NOS terminator 가 삽입되어 PPO 유전자의 전사 종결을 유도하였다. 또한, 엽록체로 단백질의 이동과 발현을 유도하기 위해 Arabidopsis thaliana 유래의 PPO 유전자인 AtPPO1 유전자 (서열번호 301)의 transit peptide (TP) 암호화 부위 (서열번호 302)를 XbaI, XhoI 제한효소를 이용하여 PPO 유전자의 5' 앞에 삽입하였다. 상기에서 제작한 각각의 벡터를 아그로박테리움 투메파시엔스 (Agrobacterium tumefaciens) GV3101 competent cell에 freeze-thaw 방법으로 도입하여 형질전환 시켰다. GV3101 competent cell 을 제조하기 위하여, GV3101 균주를 5 ml LB media 에 30 ℃ 및 200 rpm 조건에서 12시간 배양하였다. 이 배양액을 200 ml LB media 에 접종하여 30℃ 및 200 rpm 조건에서 3~4시간 배양하고, 3000 xg, 4℃ 에서 20분간 원심분리 하였다. 멸균 증류수로 pellet을 씻어 준 뒤 20 ml LB media 로 재현탁 (resuspension)하였다. 200 ㎕씩 aliquot 하여 액체 질소에 snap freezing 한 뒤, 초저온 냉동고에 보관하였다.
각각의 형질전환 아그로박테리움을 항생제 배지 (spectinomycin을 포함한 LB agar)에서 배양 및 선별하였다. 상기 선별된 콜로니를 LB broth에서 액체 배양하였다. 이 배양액으로부터 아그로박테리움 cell을 harvest 한 후, 5% (w/v) sucrose 용액에 흡광도 (OD600) 0.8의 농도로 현탁한 후, 0.05% (v/v) Silwet L-77 (Momentive Performance Materials)를 첨가하였다. Floral dipping 방법으로 Col-0 애기장대 wild type에 형질전환하여 형질전환 애기장대를 제작하고, 1~2달 후 종자 (T1)를 수확하였다.
형질전환 개체 선발을 위해 삽입된 Bar 유전자를 이용하여 형질전환 개체를 선발하였다. 상기 얻어진 T1 종자를 50 uM glufosinate가 첨가된 1/2 MS 배지 (2.25g/l MS salt, 10g/l sucrose, 7g/l Agar)에 파종하고, 파종 7일 후 생존한 개체를 선발하여 흙으로 이식하고 성장시켜 T1 식물체를 수득하였다.
이식한 T1 식물체들의 PPO 저해 제초제 내성을 판단하기 위해 3~4주 키운 후 추대 전 tiafenacil을 처리하였다. 40 x 60 cm 면적 (0.24 m2) 에 tiafenacil 용액 100 ml (1 uM tiafenacil + 0.05%(v/v) Silwet L-77)을 골고루 분사하였다. 야생형 애기장대 (Col-0)의 경우 상기와 동일한 농도로 tiafenacil을 처리하면 사멸하는 반면, 형질전환 식물들은 PPO 저해 제초제 처리에 저항성을 보이며 생존하였다.
내성을 나타내어 살아남는 식물체는 지속적으로 키워 종자를 수확하였고 (T2 종자), 상기 T2 종자를 50 uM glufosinate를 첨가한 1/2 MS 배지 (2.25g/l MS salt, 10g/l sucrose, 7g/l Agar)에 파종하여 7일간 키운 뒤, 생존 개체를 선발하였다.
6-2. 형질전환 애기장대(T
2
)의 제초제 저항성 검증
CyPPO19 WT, CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V), CyPPO18 WT, 또는 CyPPO18 변이 (L327T+F360M)를 암호화하는 유전자가 각각 삽입된 애기장대 형질전환체 (T2)에 대하여 제초제 내성을 테스트하였다.
CyPPO19 WT, CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자가 형질전환 된 애기장대 개체 (T2) 재배면적, 40 x 60 cm (0.24 m2) 당, tiafenacil 용액 100 ml (1 μM tiafenacil + 0.05% (v/v) Silwet L-77) 또는 flumioxazin 용액 100 ml (1 μM flumioxazin + 0.05% (v/v) Silwet L-77) 을 처리하고, 7일 후에 식물의 약해 정도를 판단하였다. 야생형 애기장대 (Col-0)를 대조군으로 사용하였다.
상기 얻어진 1 μM tiafenacil 또는 1 μM flumioxazin 처리 후 7일째의 애기장대 형질전환체(T2)의 성장과 약해 정도를 도 34 및 도 35에 나타내었다.
CyPPO19 변이 (F360M, F360V, F360L, V165C+F360M, V165S+F360V) 유전자가 각각 형질전환 된 애기장대 개체 (T2) 재배면적, 40 x 60 cm (0.24 m2) 당, tiafenacil 용액 100 ml (5 μM tiafenacil + 0.05% (v/v) Silwet L-77) 또는 flumioxazin 용액 100 ml (5 μM flumioxazin + 0.05% (v/v) Silwet L-77) 을 처리하고, 7일 후에 식물의 약해 정도를 판단하였다. 야생형 애기장대 (Col-0)를 대조군으로 사용하였다.
상기 얻어진 5 μM tiafenacil 또는 5 μM flumioxazin 처리 후 7일째의 애기장대 형질전환체(T2)의 성장과 약해 정도를 도 36 및 도 37에 나타내었다.
CyPPO18 WT 유전자가 형질전환 된 애기장대 개체 (T2) 재배면적, 40 x 60 cm (0.24 m2) 당, tiafenacil 용액 100 ml (1 μM tiafenacil + 0.05% (v/v) Silwet L-77) 을 처리하고, 7일 후에 식물의 약해 정도를 판단하였다. 야생형 애기장대 (Col-0)를 대조군으로 사용하였다.
상기 얻어진 1 μM tiafenacil 처리 후 7일째의 애기장대 형질전환체(T2)의 성장과 약해 정도를 도 38에 나타내었다.
CyPPO18 변이 (L327T+F360M) 유전자가 형질전환 된 애기장대 개체 (T2) 재배면적, 40 x 60 cm (0.24 m2) 당, tiafenacil 용액 100 ml (1 μM tiafenacil + 0.05% (v/v) Silwet L-77) 을 처리하고, 7일 후에 식물의 약해 정도를 판단하였다. 야생형 애기장대 (Col-0)를 대조군으로 사용하였다.
상기 얻어진 1 μM tiafenacil 처리 후 7일째의 애기장대 형질전환체(T2)의 성장과 약해 정도를 도 39에 나타내었다.
상기 도 34 내지 도 39 결과를 기초로, 각 형질전환 식물체의 제초제 내성을 아래의 표 15에 기재된 Injury index 기준에 의하여 평가하였다:
Injury index | 증상 |
0 | 약해가 없음 |
1 | 잎 끝이 마른 상태 또는 20% 미만이 누렇게 마름 |
2 | 식물체의 20% 이상 30% 미만이 누렇게 마름 |
2.5 | 식물체의 30% 이상 50% 미만이 누렇게 마름 |
3 | 식물체의 50% 이상 70% 미만이 누렇게 마름 |
4 | 식물체의 70% 이상이 누렇게 마름 |
5 | 식물 전체가 말라서 죽음 |
상기와 같이 평가된 각 형질전환 식물체의 제초제 약해 수준 (Injury index)을 아래의 표 16 내지 표 19에 나타내었다.
Injury index | ||
1 μM tiafenacil 처리 | 1 μM flumioxazin 처리 | |
Col-0 | 5 | 5 |
CyPPO19 WT | 3 | 2 |
CyPPO19 F360M | 0-1 | 0-1 |
CyPPO19 F360V | 0-1 | 0-1 |
CyPPO19 F360L | 1 | 1 |
CyPPO19 V165C+F360M | 0-1 | 0-1 |
CyPPO19 V165S+F360V | 0-1 | 0-1 |
Injury index | ||
5 μM tiafenacil 처리 | 5 μM flumioxazin 처리 | |
Col-0 | 5 | 5 |
CyPPO19 F360M | 1 | 1 |
CyPPO19 F360V | 0-1 | 0-1 |
CyPPO19 F360L | 1 | 1 |
CyPPO19 V165C+F360M | 0-1 | 0-1 |
CyPPO19 V165S+F360V | 0-1 | 0-1 |
Injury index | |
1 μM tiafenacil 처리 | |
Col-0 | 5 |
CyPPO18 WT | 3-4 |
Injury index | |
1 μM tiafenacil 처리 | |
Col-0 | 5 |
CyPPO18 L327T+F360M | 0-1 |
상기 결과에서 보여지는 바와 같이, CyPPO19 WT 또는 CyPPO18 WT이 형질전환된 식물은 형질전환되지 않은 식물과 비교하여 제초제에 대한 저항성이 증진되며, CyPPO19 변이체 또는 CyPPO18 변이체가 형질전환된 식물은 형질전환되지 않은 식물보다 현저히 증진된 제초제 저항성을 가짐을 확인할 수 있다.
<110> FarmHannong Co., Ltd.
<120> Methods and Compositions for Conferring and/or Enhancing
Herbicide Tolerance Using Protoporphyrinogen IX Oxidase of
Various Cyanobacteria or Variant Thereof
<130> DPP20201307KR
<150> 10-2019-0071028
<151> 2019-06-14
<160> 302
<170> koPatentIn 3.0
<210> 1
<211> 467
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Thermosynechococcus elongatus PKUAC-SCTE542
(comprising GenBank No. CP032152.1)
<400> 1
Met Ser Glu Val Asp Val Ala Ile Val Gly Gly Gly Leu Ser Gly Leu
1 5 10 15
Ser Leu Ala Trp Arg Leu Gln Gln Ser Ala Pro Gln Tyr Ser Val Val
20 25 30
Leu Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr Thr Gln Thr
35 40 45
Ala Glu Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe Ala Pro Thr
50 55 60
Pro Ala Leu Leu Gln Leu Ile Ala Glu Val Gly Leu Gln Ser Glu Leu
65 70 75 80
Ile Arg Gly Asp Arg His Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Gln
85 90 95
Leu Tyr Pro Leu Gln Pro Thr Arg Pro Leu Ala Leu Ala Thr Ser Asn
100 105 110
Leu Leu Ser Pro Trp Gly Lys Val Arg Ala Ala Leu Gly Ala Leu Gly
115 120 125
Phe Val Pro Pro Tyr Leu Gly Ser Gly Asp Glu Ser Val Asn Ser Phe
130 135 140
Phe Arg Arg His Leu Gly Gln Glu Val Ala Glu Arg Leu Val Ala Pro
145 150 155 160
Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu Ser Ala Ala
165 170 175
Ala Ala Phe Arg Arg Ile Ala Gln Leu Glu Lys Leu Gly Gly Gly Leu
180 185 190
Ile Ala Gly Ala Leu His Leu Arg Arg Gln Gln Ala Pro Lys Pro Lys
195 200 205
Pro Pro Thr Ser Val Gln Met Arg Pro Gly Glu Leu Gly Ser Phe Lys
210 215 220
Glu Gly Leu Ala Ala Leu Pro Arg Ala Ile Ala Gln Gln Leu Lys Ala
225 230 235 240
Pro Ile His Leu Gln Thr Pro Val Gln Glu Ile Thr Pro Asp Pro Lys
245 250 255
Gly Gly Tyr Leu Leu Arg Ser Gly Glu Gln Thr Trp Arg Ala Arg Ser
260 265 270
Val Val Leu Ala Thr Pro Ala Tyr Gln Thr Ala Glu Leu Val Ala Pro
275 280 285
Phe Gln Pro Ala Ile Ala Arg Val Leu Ala Thr Ile Pro Tyr Pro Thr
290 295 300
Val Ala Cys Val Val Leu Ala Tyr Pro Ala Gly Leu Gly Arg Ser Val
305 310 315 320
Arg Pro Gly Phe Gly Val Leu Ile Pro Arg Ser Gln Gly Ile Arg Thr
325 330 335
Leu Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gln Arg Thr Pro Ala
340 345 350
Gly Trp Gln Val Phe Thr Ser Phe Ile Gly Gly Ala Thr Asp Pro Asp
355 360 365
Leu Ala Ser Leu Ser Glu Glu Ala Ile Val Gln Gln Val Gln Gln Asp
370 375 380
Leu Asn Arg Leu Leu Asp Leu Pro Ala Ala Lys Ala Arg Leu Leu Gly
385 390 395 400
Met Lys Val Trp Arg Arg Ala Ile Pro Gln Tyr Met Val Gly Tyr Pro
405 410 415
Glu Gln Trp Gln Gln Val Thr His Ala Leu Ser Gln Thr Pro Gly Leu
420 425 430
Phe Leu Cys Ser Asn Tyr Ala Glu Gly Val Ala Leu Gly Asp Arg Val
435 440 445
Glu His Gly Asn Arg Thr Ala Ala Ala Val Ala Ala Tyr Leu Ser Gly
450 455 460
Gly Gln Pro
465
<210> 2
<211> 465
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanobacteria bacterium J003 (GenBank: RMH63851.1)
<400> 2
Met Met Glu Val Asp Val Ala Ile Val Gly Gly Gly Leu Ser Gly Leu
1 5 10 15
Ser Val Ala Trp Arg Leu Gln Gln Ser Ala Pro Gln Tyr Ser Gly Val
20 25 30
Leu Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr Thr Gln Gly
35 40 45
Ala Glu Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe Ala Pro Thr
50 55 60
Pro Ala Leu Leu Gln Leu Ile Ala Glu Val Gly Leu His Ser Glu Leu
65 70 75 80
Ile Arg Gly Asp Arg His Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Glu
85 90 95
Leu Tyr Pro Leu Glu Pro Thr Arg Pro Leu Ala Leu Ala Thr Ser Asn
100 105 110
Leu Leu Ser Pro Trp Gly Lys Val Arg Ala Ala Leu Gly Ala Leu Gly
115 120 125
Phe Val Pro Pro Tyr Leu Gly Ser Gly Asp Glu Ser Val Asn Ser Phe
130 135 140
Phe Arg Arg His Leu Gly Gln Glu Val Ala Glu Arg Leu Val Ala Pro
145 150 155 160
Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu Ser Ala Ala
165 170 175
Ala Ala Phe Arg Arg Ile Ala Gln Leu Glu Lys Leu Gly Gly Gly Leu
180 185 190
Ile Ala Gly Ala Leu Arg Leu Arg Arg Gln Gln Pro Pro Lys Pro Arg
195 200 205
Pro Pro Ala Glu Val Gln Met Arg Pro Gly Glu Leu Gly Ser Phe Lys
210 215 220
Glu Gly Leu Ala Ala Leu Pro Arg Ala Ile Ala Gln Gln Leu Lys Ala
225 230 235 240
Pro Val His Leu Gln Thr Pro Val Glu Ala Ile Thr Pro Glu Pro Asn
245 250 255
Gly Gly Tyr Leu Leu Arg Ser Gly Glu Gln Thr Trp Gln Ala Arg Ser
260 265 270
Val Val Leu Ala Thr Pro Ala Tyr Gln Thr Ala Ala Leu Val Ala Pro
275 280 285
Phe Gln Pro Ala Ile Ala Arg Val Leu Ala Ala Ile Pro Tyr Pro Thr
290 295 300
Val Ala Cys Val Val Leu Ala Tyr Pro Ala Gly Leu Gly Arg Ser Val
305 310 315 320
Arg Pro Gly Phe Gly Val Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr
325 330 335
Leu Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gln Arg Thr Pro Ala
340 345 350
Gly Trp Gln Val Phe Thr Ser Phe Ile Gly Gly Ala Thr Asp Pro Asp
355 360 365
Leu Ala Ser Leu Arg Glu Glu Ala Ile Val Gln Gln Val Gln Gln Asp
370 375 380
Leu Thr Arg Leu Leu Asp Leu Pro Ala Ala Lys Ala Arg Leu Leu Gly
385 390 395 400
Met Lys Val Trp Arg Arg Ala Ile Pro Gln Tyr Leu Val Gly Tyr Pro
405 410 415
Gln Gln Trp Gln Gln Val Thr His Ala Leu Ser His Thr Pro Gly Leu
420 425 430
Phe Leu Cys Ser Asn Tyr Ala Glu Gly Val Ala Leu Gly Asp Arg Val
435 440 445
Glu His Gly Asn Arg Thr Ala Ala Ala Val Ala Ala Tyr Leu Ser Gly
450 455 460
Gly
465
<210> 3
<211> 467
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Thermosynechococcus vulcanus NIES-2134 (BAY51976)
<400> 3
Met Ile Glu Val Asp Val Ala Ile Val Gly Gly Gly Leu Ser Gly Leu
1 5 10 15
Ser Val Ala Trp Arg Leu Gln Arg Ser Ala Pro His Tyr Ser Gly Val
20 25 30
Leu Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr Thr Gln Ala
35 40 45
Ala Glu Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe Ala Pro Thr
50 55 60
Pro Ala Leu Leu Gln Leu Ile Ala Glu Val Gly Leu His Ser Glu Leu
65 70 75 80
Ile Arg Gly Asp Arg His Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Glu
85 90 95
Leu Tyr Pro Leu Glu Pro Thr Arg Pro Leu Ala Leu Ala Thr Ser Asn
100 105 110
Leu Leu Ser Pro Trp Gly Lys Val Arg Ala Ala Leu Gly Ala Leu Gly
115 120 125
Phe Val Pro Pro Tyr Leu Gly Ser Gly Asp Glu Ser Val Asp Ser Phe
130 135 140
Phe Arg Arg His Leu Gly Gln Glu Val Ala Glu Arg Leu Val Ala Pro
145 150 155 160
Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu Ser Ala Ala
165 170 175
Ala Ala Phe Arg Arg Ile Ala Gln Leu Glu Lys Leu Gly Gly Ser Leu
180 185 190
Ile Ala Gly Ala Leu Arg Leu Arg Arg Gln Gln Pro Pro Gln Pro Lys
195 200 205
Pro Pro Ala Gln Val Gln Met Arg Pro Gly Glu Leu Gly Ser Phe Arg
210 215 220
Glu Gly Leu Ala Ala Leu Pro Arg Ala Ile Ala Gln Gln Leu Lys Ala
225 230 235 240
Pro Leu His Leu Gln Thr Pro Val Glu Ala Ile Thr Pro Glu Pro Lys
245 250 255
Gly Gly Tyr Leu Leu Arg Ser Gly Glu Gln Thr Trp His Ala Arg Ser
260 265 270
Val Val Leu Ala Thr Pro Ala Tyr Gln Ser Ala Glu Leu Val Ala Pro
275 280 285
Phe Gln Pro Ala Ile Ala Arg Ala Leu Ala Thr Ile Pro Tyr Pro Thr
290 295 300
Val Ala Cys Val Val Leu Ala Tyr Pro Ala Gly Leu Gly Arg Ser Val
305 310 315 320
Arg Pro Gly Phe Gly Val Leu Val Pro Arg Gly Gln Gly Ile Arg Thr
325 330 335
Leu Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gln Arg Thr Pro Ala
340 345 350
Gly Trp Gln Val Phe Thr Ser Phe Ile Gly Gly Ala Thr Asp Pro Asp
355 360 365
Leu Ala Ser Leu Arg Glu Glu Ala Ile Val Glu Gln Val Gln Gln Asp
370 375 380
Leu Thr Arg Leu Leu Asp Leu Pro Ala Ala Lys Ala Arg Leu Leu Gly
385 390 395 400
Met Lys Val Trp Arg Arg Ala Ile Pro Gln Tyr Ile Val Gly Tyr Pro
405 410 415
Gln Gln Trp Gln Gln Leu Thr His Ala Leu Thr Gln Thr Pro Gly Leu
420 425 430
Phe Leu Cys Ser Asn Tyr Ala Glu Gly Val Ala Leu Gly Asp Arg Val
435 440 445
Glu His Gly Asn Arg Thr Ala Ala Ala Val Ala Ala Tyr Leu Ala Gly
450 455 460
Gly Gln Ser
465
<210> 4
<211> 476
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus lividus (WP_099798264)
<400> 4
Met Ser Glu Pro Ile Val Val Asp Val Ala Val Val Gly Ala Gly Leu
1 5 10 15
Ser Gly Leu Ser Val Ala Trp Arg Leu Gln Gln Val Ala Pro Gln Tyr
20 25 30
Lys Val Val Val Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr
35 40 45
Thr Glu Ala Arg Asp Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe
50 55 60
Ala Pro Thr Pro Ala Leu Leu His Leu Ile Ala Glu Val Gly Leu Gln
65 70 75 80
Asp Gln Leu Leu Arg Gly Asp Arg Arg Leu Pro Arg Tyr Ile Tyr Trp
85 90 95
Arg Gly Lys Leu His Pro Leu Glu Pro Thr Arg Pro Leu Ala Leu Ala
100 105 110
Thr Ser Gly Leu Leu Ser Pro Trp Gly Lys Leu Arg Ala Ala Leu Gly
115 120 125
Ala Phe Gly Phe Val Pro Pro Tyr Leu Arg Asp Ala Asp Glu Ser Val
130 135 140
Ser Ser Phe Phe Gln Arg His Leu Gly Lys Glu Val Ala Glu Arg Leu
145 150 155 160
Val Ala Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu
165 170 175
Ser Ala Ala Ala Ala Phe Arg Arg Ile Val Gln Leu Glu Gln Leu Gly
180 185 190
Gly Ala Leu Ile Pro Gly Ala Leu Arg Leu Arg Arg Gln Gln Pro Pro
195 200 205
Lys Pro Thr Pro Pro Lys Gly Leu Glu Met Arg Pro Gly Glu Leu Gly
210 215 220
Ser Phe Gln Glu Gly Leu Ser Ala Leu Pro Arg Ala Ile Ala His His
225 230 235 240
Leu Ala Ala Pro Ile His Leu Gln Thr Ala Leu His Gln Leu Thr Pro
245 250 255
Glu Leu Ser Gly Gly Tyr Thr Leu Ser Ala Thr Thr Pro Asp Gly Glu
260 265 270
Gln Ala Trp Arg Ala Arg Ser Val Val Leu Ala Thr Pro Ala Tyr Val
275 280 285
Thr Ala Asp Leu Leu Arg Pro Trp Gln Pro Thr Ile Ala Ala Gly Leu
290 295 300
Glu Ala Ile Pro Tyr Pro Ala Val Ala Cys Val Val Leu Ala Tyr Pro
305 310 315 320
Ala Ala Val Gly Val Thr Ala Arg Pro Gly Phe Gly Val Leu Ile Pro
325 330 335
Arg Thr Gln Gly Leu Arg Thr Leu Gly Thr Ile Trp Ser Ser Cys Leu
340 345 350
Phe Pro Glu Arg Thr Pro Ser Gly Trp Gln Val Phe Thr Ser Phe Ile
355 360 365
Gly Gly Ala Thr Asp Pro Glu Leu Ala Thr Leu Glu Pro Glu Ala Ile
370 375 380
Val Gln Gln Val Gln Gln Asp Leu Glu His Met Leu Ala Leu Pro Pro
385 390 395 400
Ala Lys Ala Arg Leu Leu Gly Met Lys Leu Trp Arg Arg Ala Ile Pro
405 410 415
Gln Tyr Thr Leu Gly Tyr Pro Gln Gln Trp Gln Gln Ile Thr His Ala
420 425 430
Leu Lys His Leu Pro Gly Leu Phe Leu Cys Ser Asn Tyr Ala Ala Gly
435 440 445
Val Ala Leu Gly Asp Arg Val Glu His Gly Tyr Asn Thr Ala Ala Ala
450 455 460
Val Asp Ala Tyr Leu Gly Gly Thr Tyr Gly Ser Val
465 470 475
<210> 5
<211> 484
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Microcoleaceae bacterium UBA10368 (HBK97224)
<400> 5
Met Thr Asn Val Val Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu Gln Lys Gln Ala Lys Thr Ala Pro Pro
20 25 30
Leu Lys Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Gln Thr Asp Gly Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ala Pro Thr Pro Glu Leu Met Lys Leu Ala Val Asp Val Gly Leu
65 70 75 80
Lys Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Gln Gly Lys Leu Gln Pro Val Pro Met Thr Pro Gln Ala Met Ile
100 105 110
Gln Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Val Gly
115 120 125
Ala Leu Gly Phe Val Gly Pro Ala Met Gly Ser Gln Leu Ser Gln Gln
130 135 140
Gly Gly Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Thr
145 150 155 160
Glu Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr
180 185 190
Gln Met Ala Asp Val Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser
195 200 205
Ala Arg Lys Arg Pro Lys Lys Leu Pro Val Asp Pro Asn Ile Pro Gln
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Lys Gln Gly Leu Lys Ala Leu
225 230 235 240
Pro Glu Ala Ile Ala Ala Gln Leu Gly Asp Arg Leu Lys Leu Asn Trp
245 250 255
His Leu Thr Arg Leu Gln Arg Thr Glu Arg Gln Thr Tyr Ile Ala Glu
260 265 270
Phe Ala Thr Pro Asp Gly Gln Gln Gln Val Glu Ala Arg Thr Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Ile Thr Ala Glu Leu Leu Ala Pro Leu Gln
290 295 300
Pro Glu Val Ser Ser Ala Leu Gln Ala Val Thr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Leu Ser Asp Val Lys Gly Lys Leu Val
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Thr Ser Ser Leu Phe Pro Asp Arg Ala Pro Ala Gly Trp
355 360 365
Gln Thr Leu Ser Asn Tyr Ile Gly Gly Ala Thr Asp Ser Asp Ile Ala
370 375 380
Asn Leu Asp Pro Glu Gln Ile Val Gly Glu Val His Arg Asp Leu Ser
385 390 395 400
Arg Ile Leu Leu Lys Pro Leu Ala Ala Gln Pro Lys Val Leu Ala Val
405 410 415
Asn Leu Trp Lys Arg Ala Ile Pro Gln Tyr Asn Leu Gly His Leu Pro
420 425 430
Arg Leu Gln Gln Val Glu Asn Gly Leu Lys Ser Leu Pro Gly Val Tyr
435 440 445
Leu Cys Ser Asn Tyr Val Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Trp Gly Phe Glu Arg Ala Ile Glu Val Ser Glu Tyr Leu Gln Glu Thr
465 470 475 480
Gly His Arg Ala
<210> 6
<211> 476
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Microcoleaceae bacterium UBA11344 (HAT14741.1)
<400> 6
Met Thr Asn Ile Val Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu Gln Lys Gln Ala Lys Thr Ala Pro Pro
20 25 30
Leu Lys Ile Leu Val Ala Glu Ser Arg Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Xaa Xaa Glu Glu Gly Pro Asn Ser Phe Met Pro Thr Pro Glu
50 55 60
Leu Met Lys Leu Ala Val Asp Val Gly Leu Lys Gln Glu Leu Ile Phe
65 70 75 80
Ala Asp Arg Lys Leu Pro Arg Tyr Ile Tyr Trp Gln Gly Lys Leu Gln
85 90 95
Pro Val Pro Met Thr Pro Gln Ala Met Ile Gln Ser Gln Leu Leu Ser
100 105 110
Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly Ala Leu Gly Phe Val Gly
115 120 125
Ser Ala Met Gly Ser Gln Leu Ser Gln Gln Gly Gly Glu Glu Thr Val
130 135 140
Ser Gln Phe Phe Arg Arg His Leu Gly Thr Glu Val Met Gln Arg Leu
145 150 155 160
Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu
165 170 175
Ser Ala Ala Ala Ala Phe Gly Arg Val Thr Gln Met Ala Asp Val Gly
180 185 190
Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala Arg Lys Arg Pro Lys
195 200 205
Lys Leu Pro Val Asp Pro Asn Ile Pro Gln Thr Lys Pro Gly Glu Leu
210 215 220
Gly Ser Phe Lys Gln Gly Leu Lys Ala Leu Pro Glu Ala Ile Ala Ala
225 230 235 240
Gln Leu Gly Asp Arg Leu Lys Leu Asn Trp His Leu Thr Arg Leu Gln
245 250 255
Arg Thr Glu Arg Gln Thr Tyr Ile Ala Glu Phe Ala Thr Pro Asp Gly
260 265 270
Gln Gln Gln Val Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr
275 280 285
Ile Thr Ala Glu Leu Leu Ala Pro Leu Gln Pro Glu Val Ser Ser Ala
290 295 300
Leu Gln Ala Val Thr Tyr Pro Thr Val Ala Cys Val Val Leu Ala Tyr
305 310 315 320
Pro Leu Ser Asp Val Lys Gly Lys Leu Val Gly Phe Gly Asn Leu Ile
325 330 335
Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Thr Ser Ser
340 345 350
Leu Phe Pro Asp Arg Ala Pro Ala Gly Trp Gln Thr Leu Ser Asn Tyr
355 360 365
Ile Gly Gly Ala Thr Asp Ser Asp Ile Ala Asn Leu Asp Pro Glu Gln
370 375 380
Ile Val Gly Glu Val His Arg Asp Leu Ser Arg Met Leu Leu Lys Pro
385 390 395 400
Leu Val Ala Gln Pro Lys Val Leu Ala Val Asn Leu Trp Lys Arg Ala
405 410 415
Ile Pro Gln Tyr Asn Leu Gly His Ile Gln Arg Leu Gln Gln Val Glu
420 425 430
Asn Gly Leu Lys Ser Leu Pro Gly Val Tyr Leu Cys Ser Asn Tyr Val
435 440 445
Gly Gly Ile Ala Leu Gly Asp Cys Val Arg Trp Gly Phe Glu Arg Ala
450 455 460
Ile Glu Val Ser Glu Tyr Leu Gln Glu Thr Gly Arg
465 470 475
<210> 7
<211> 491
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAE85894)
<400> 7
Met Glu Val Leu Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu
1 5 10 15
Ser Leu Ala His Ala Leu Gln Lys Glu Ala Arg Ile Ala Ser Pro Arg
20 25 30
Ala Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Ala Thr Gly Glu Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp
85 90 95
Asp Lys Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile Gln
100 105 110
Ser Gly Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly Ala
115 120 125
Leu Gly Phe Val Ala Pro Ala Met Gly Ser Gln Leu Ser Gln Gln Gly
130 135 140
Asp Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Lys Glu
145 150 155 160
Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr Lys
180 185 190
Met Ala Asp Ala Gly Gly Ser Leu Val Ala Gly Ala Leu Leu Ser Ala
195 200 205
Arg Lys Lys Lys Pro Gln Pro Pro Leu Gly Lys Gly Gly Leu Asn Ala
210 215 220
Leu Ala Asp Pro Asn Ile Pro Lys Thr Lys Arg Gly Glu Leu Gly Ser
225 230 235 240
Phe Lys Gly Gly Leu Lys Ala Leu Pro Glu Ala Ile Ala Ala Ser Leu
245 250 255
Gly Asp Arg Val Lys Leu Asn Trp His Leu Thr Arg Leu Asp Arg Thr
260 265 270
Glu Arg Glu Thr Tyr Ile Ala Val Phe Ser Thr Pro Asp Gly Gln Gln
275 280 285
Glu Ile Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr Val Thr
290 295 300
Ala Glu Leu Leu Gln Pro Leu Gln Pro Ser Val Ser Ser Ala Leu Gln
305 310 315 320
Ala Phe Thr Tyr Pro Thr Val Ala Ser Val Val Leu Ala Tyr Pro Met
325 330 335
Ser Asp Val Lys Gly Lys Leu Val Gly Phe Gly Asn Leu Ile Pro Arg
340 345 350
Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe
355 360 365
Pro Asp Arg Ala Pro Ala Gly Trp Gln Thr Leu Thr Ser Tyr Ile Gly
370 375 380
Gly Ala Thr Asp Ser Gly Ile Gly Asn Leu Asp Ala Glu Gln Ile Val
385 390 395 400
Gly Glu Val His Arg Asp Leu Ser Arg Ile Leu Leu Lys Pro Glu Ala
405 410 415
Ala Gln Pro Lys Val Leu Thr Val Lys Leu Trp Lys Arg Ala Ile Pro
420 425 430
Gln Tyr Asn Leu Gly Tyr Phe Asp Arg Leu Gln Gln Ile Asp Arg Gly
435 440 445
Leu Lys Ser Leu Pro Gly Leu Tyr Leu Cys Ser Asn Tyr Leu Gly Gly
450 455 460
Val Ala Leu Gly Asp Cys Val Arg Arg Gly Phe Glu Arg Ala Gln Glu
465 470 475 480
Val Gly Glu Tyr Leu Asn Asp Ser Phe Glu Phe
485 490
<210> 8
<211> 467
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanobacteria bacterium UBA8156 (HAN47127.1)
<400> 8
Met Asp Asp Arg Gln Gly Glu Pro Thr Glu Val Leu Val Val Gly Ala
1 5 10 15
Gly Ile Cys Gly Leu Thr Val Ala His Gly Leu Ala Gln Arg Gly Val
20 25 30
Ala Cys Arg Val Val Glu Ala Ser Asp Arg Val Gly Gly Ala Ile Val
35 40 45
Thr Arg Arg Glu His Gly Phe Gln Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Ala Leu Ala Ile Ala Met Gly Leu Arg
65 70 75 80
Asp Glu Leu Ile Leu Ala Asp Arg Arg Leu Pro Arg Tyr Val Trp Trp
85 90 95
Gln Asn Arg Leu Gln Ala Val Pro Met Ala Pro Pro Gly Leu Leu Thr
100 105 110
Thr Gly Leu Leu Ser Pro Trp Gly Lys Leu Arg Ala Ala Val Gly Ala
115 120 125
Leu Gly Phe Val Pro Pro Ser Phe Ala Ala Asp Glu Thr Val Gly Ser
130 135 140
Phe Phe Arg Arg His Leu Gly Pro Glu Val Leu Thr Arg Leu Ala Ala
145 150 155 160
Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Glu Ala Leu Ser Ile
165 170 175
Gly Ala Ala Phe Pro Arg Val Thr Ala Ile Glu Ala Met Gly Gly Gly
180 185 190
Leu Val Ala Gly Phe Ile Gln Ala Leu Arg Gln Arg Gly Ala Pro Asp
195 200 205
Pro His Leu Pro Gln Thr Lys Pro Gly Glu Leu Gly Ser Phe Arg Glu
210 215 220
Gly Ile Glu Ala Leu Pro Arg Ala Ile Ala Ala Asp Leu Ala Gln Arg
225 230 235 240
Gly Val Thr Leu Thr Leu Asn Gln Ala Leu Thr Arg Leu Glu Pro Thr
245 250 255
Gly Asp Arg Trp Arg Ala Val Leu Ala Ser Gly Glu Glu Leu Glu Ala
260 265 270
Arg Ser Val Val Leu Ala Val Pro Ala Trp Ala Ala Ala Lys Ile Leu
275 280 285
Gln Gln Thr Pro Glu Thr Ala Asp Trp Val Ala Asp Leu Glu Arg Ile
290 295 300
Pro Tyr Pro Ala Val Ala Cys Ala Val Leu Ala Tyr Pro Asp Gly Asp
305 310 315 320
Leu Arg Gln Pro Leu Arg Gly Phe Gly His Leu Val Pro Arg Gly Gln
325 330 335
Gly Ile Arg Thr Leu Gly Thr Ile Trp Ala Ser Ser Leu Phe Pro Gly
340 345 350
Arg Ala Pro Ala Gly Tyr Thr Leu Leu Leu Asn Phe Ile Gly Gly Thr
355 360 365
Thr Asp Pro Thr Leu Ala Gln Leu Asp Ala Gly Ala Ile Ala Gln Ala
370 375 380
Val His Gln Asp Leu Arg Gln Ile Leu Val Gln Pro Thr Ala Ala Pro
385 390 395 400
Pro Lys Val Leu Ala Val Asn Leu Trp Arg Arg Ala Ile Pro Gln Phe
405 410 415
Thr Val Gly His Gln Gly Arg Leu Ala Arg Leu Ala Ala Gly Leu Pro
420 425 430
Pro Gly Leu Val Leu Ala Gly Asn Tyr Arg Gly Gly Val Ala Leu Gly
435 440 445
Asp Cys Val Arg His Gly Leu Ala Val Ala Ala Gln Val Ala Ala Phe
450 455 460
Leu Glu Arg
465
<210> 9
<211> 469
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Rubidibacter lacunae (WP_022605844)
<400> 9
Met Leu Asp Thr Leu Val Val Gly Ala Gly Leu Ser Gly Leu Ser Val
1 5 10 15
Ala Arg Ser Leu Gln Met Ala Gly Arg Asn Val Leu Val Ala Glu Ala
20 25 30
Gln Glu Arg Val Gly Gly Asn Ile Leu Thr Gln Gln Ser Asp Asp Gly
35 40 45
Phe Gln Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro Asn Arg Glu Leu
50 55 60
Leu Glu Leu Ala Val Gly Val Gly Leu Arg Asp Lys Leu Ile Phe Ala
65 70 75 80
Asp Arg Arg Leu Pro Arg Phe Val Tyr Trp Arg Asn Ser Leu His Pro
85 90 95
Val Pro Met Ser Pro Pro Arg Ala Val Thr Thr Ser Leu Leu Ser Pro
100 105 110
Leu Gly Lys Leu Arg Ala Val Ala Gly Ala Ile Gly Phe Val Pro Pro
115 120 125
Ala Leu Ser Asp Glu Glu Ser Val Ala Glu Phe Phe Thr Arg His Leu
130 135 140
Gly Ser Glu Val Ala Glu Arg Leu Val Ala Pro Phe Val Ser Gly Val
145 150 155 160
Tyr Ala Gly Asp Val Glu Arg Leu Ser Val Ser Ser Ala Phe Arg Gln
165 170 175
Val Ala Lys Leu Ser Glu Val Gly Gly Gly Leu Leu Ala Gly Ala Leu
180 185 190
Leu Thr Arg Arg Gly Lys Ser Gly Pro Pro Gln Lys Leu Pro Pro Arg
195 200 205
Val Asp Pro Asn Leu Pro Arg Val Gln Arg Gly Glu Leu Gly Ser Phe
210 215 220
Val Gly Gly Leu Lys Met Leu Pro Glu Ala Ile Ala Ala Lys Leu Gly
225 230 235 240
Thr Lys Leu Lys Leu His Trp Thr Leu Glu His Leu Val Arg Cys Glu
245 250 255
Gly Gly Tyr Arg Ala Glu Phe Ala Thr Pro Glu Gly Gln Gln Gln Ile
260 265 270
Glu Val Arg Ser Leu Val Leu Ala Thr Pro Ala Tyr Arg Cys Ala Arg
275 280 285
Val Leu Gln Ser Leu Ala Pro Ser Ala Cys Gln Ala Leu Ser Glu Met
290 295 300
Pro Tyr Pro Ala Val Ala Cys Val Val Leu Ala Tyr Pro Ser Ser Ala
305 310 315 320
Phe Ser Ser Pro Leu Gln Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln
325 330 335
Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ala Leu Phe Pro Gly
340 345 350
Arg Thr Pro Pro Gly Trp Glu Ile Leu Thr Ser Phe Ile Gly Gly Ala
355 360 365
Thr Asp Pro Glu Leu Gly Thr Leu Ser Ala Gln Gln Ile Val Ala Glu
370 375 380
Val Arg Arg Asp Leu Gln Arg Val Leu Val Asp Lys Glu Pro Asn Thr
385 390 395 400
Glu Pro Arg Val Leu Ala Ala Lys Val Trp Pro Arg Ala Ile Pro Gln
405 410 415
Tyr Val Leu Gly His Ser Asp Arg Leu Asn Arg Ile Gln Ile Asp Leu
420 425 430
Asp Arg Leu Pro Gly Leu Tyr Leu Cys Ser Asn Phe Thr Asp Gly Val
435 440 445
Ala Leu Gly Asp Cys Val Arg Arg Gly Phe Glu Thr Ala Glu Ala Ile
450 455 460
Gly Gln Tyr Leu Asn
465
<210> 10
<211> 471
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Hydrocoleum sp. CS-953 (WP_094676324)
<400> 10
Met Leu Lys Leu Ala Val Asp Val Gly Leu Lys Gln Asp Leu Ile Phe
1 5 10 15
Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp Asn Gly Gln Leu Xaa
20 25 30
Leu Val Ala Glu Ser Gln Asn Arg Val Gly Gly Asn Ile Thr Thr Val
35 40 45
Ser Gln Gly Asp Phe Ile Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro
50 55 60
Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys Gln Asp
65 70 75 80
Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp Asn Gly
85 90 95
Gln Leu Leu Pro Val Pro Met Gly Pro Thr Ala Met Leu Gln Ser Lys
100 105 110
Leu Leu Ser Asp Ser Gly Lys Leu Arg Ala Leu Val Gly Ala Leu Gly
115 120 125
Phe Val Pro Pro Ala Val Gly Thr Gly Leu Ser Gln Gln Gly Gly Glu
130 135 140
Glu Thr Val Ser Gln Phe Phe Gln Arg His Leu Gly Val Glu Val Met
145 150 155 160
Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro
165 170 175
Ser Gln Leu Ser Ala Thr Ala Ala Phe Ser Arg Val Ala Arg Met Ala
180 185 190
Asp Ile Gly Gly Gly Leu Leu Ala Gly Ala Val Leu Ser Ala Lys Arg
195 200 205
Asn Pro Lys Ser Lys Val Ala Ala Asp Pro Asn Ile Pro Lys Thr Lys
210 215 220
Pro Gly Glu Leu Gly Ser Phe Arg Gly Gly Leu Glu Ala Leu Pro Lys
225 230 235 240
Ala Ile Ala Thr Tyr Leu Gly Glu Ala Val Lys Leu Asn Trp His Leu
245 250 255
Ile Gly Ile Arg Arg Thr Glu Gln Gln Thr Tyr Ile Ala Glu Phe Ser
260 265 270
Thr Pro Asn Gly Ser Glu Gln Ile Glu Thr Arg Thr Ile Ala Leu Ser
275 280 285
Thr Pro Ala Tyr Phe Cys Ser Glu Leu Phe Lys Pro Leu Leu Pro Glu
290 295 300
Ile Ala Ser Thr Phe Asp Glu Phe Tyr Tyr Pro Thr Val Ala Cys Val
305 310 315 320
Val Leu Ala Tyr Pro Val Ser Ser Ile Lys Ala Lys Ile Asp Gly Phe
325 330 335
Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile
340 345 350
Trp Ser Ser Ala Leu Phe Pro Gly Arg Thr Pro Pro Gly Trp Gln Val
355 360 365
Phe Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro Gly Ile Ser Gln Leu
370 375 380
Asp Ser Glu Ala Ile Val Ser Arg Val His Gln Asp Leu Gly Gln Thr
385 390 395 400
Leu Leu Lys Gln Asp Ala Glu Gln Pro Lys Val Leu Ala Val His Leu
405 410 415
Trp Ser Arg Ala Ile Pro Gln Tyr Asn Leu Gly His Asn Ser Arg Leu
420 425 430
Asp Gln Ile Asn His Gly Leu Lys Ser Trp Pro Gly Val Tyr Leu Cys
435 440 445
Ser Asn Tyr Ile Gly Gly Val Ala Leu Gly Asp Cys Val Xaa Tyr Ile
450 455 460
Lys Thr Leu Gly Lys Leu Tyr
465 470
<210> 11
<211> 487
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAE55813)
<400> 11
Met Glu Val Leu Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu
1 5 10 15
Ser Leu Ala His Ala Leu Gln Lys Glu Ala Thr Ser Ala Ser Pro Arg
20 25 30
Ala Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Ala Thr Gly Glu Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp
85 90 95
Glu Lys Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile Gln
100 105 110
Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly Ala
115 120 125
Leu Gly Phe Val Gly Pro Ala Met Gly Ser Gln Leu Ser Gln Gln Gly
130 135 140
Asp Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Lys Glu
145 150 155 160
Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr Lys
180 185 190
Met Ala Asp Ala Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala
195 200 205
Arg Lys Arg Pro Pro Gln Pro Pro Leu Thr Lys Gly Gly Leu Lys Thr
210 215 220
Val Ala Asp Pro Asn Ile Pro Lys Thr Lys Pro Gly Glu Leu Gly Ser
225 230 235 240
Phe Lys Gly Gly Leu Gln Ala Leu Pro Glu Ala Ile Ala Ala Asn Leu
245 250 255
Gly Asp Arg Leu Lys Leu Asn Trp His Leu Thr Arg Leu Asp Arg Thr
260 265 270
Glu Arg Asp Thr Tyr Ile Ala Val Phe Ser Thr Pro Asp Gly Gln Gln
275 280 285
Glu Val Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr Val Thr
290 295 300
Ala Glu Leu Leu Glu Pro Leu Gln Pro Ser Val Ser Ser Ala Leu Gln
305 310 315 320
Ala Phe Thr Tyr Pro Thr Val Ala Ser Val Val Leu Ala Tyr Pro Met
325 330 335
Ser Asp Leu Lys Gly Lys Leu Val Gly Phe Gly Asn Leu Ile Pro Arg
340 345 350
Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe
355 360 365
Ala Asp Arg Ala Pro Ala Gly Trp Gln Thr Leu Thr Ser Tyr Ile Gly
370 375 380
Gly Ala Thr Asp Ser Gly Ile Gly Asn Leu Asp Ala Glu Gln Ile Val
385 390 395 400
Gly Glu Val His Arg Asp Leu Ser Gln Ile Leu Leu Lys Pro Glu Ala
405 410 415
Ala Gln Pro Lys Val Leu Thr Val Lys Ile Trp Lys Arg Ala Ile Pro
420 425 430
Gln Tyr Asn Leu Gly His Phe Asp Arg Leu Gln Gln Ile Asp Arg Gly
435 440 445
Leu Lys Ser Leu Pro Gly Leu Tyr Leu Cys Thr Asn Tyr Phe Gly Gly
450 455 460
Val Ala Leu Gly Asp Cys Val Arg Arg Gly Phe Glu Arg Ala Gln Glu
465 470 475 480
Val Asp Glu Tyr Leu Asn Gly
485
<210> 12
<211> 475
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Crinalium epipsammum (WP_015202233)
<400> 12
Met Val Asp Thr Leu Ile Ile Gly Ala Gly Ile Ser Gly Leu Ser Leu
1 5 10 15
Ala Tyr Ala Leu His Gln Asp Gly Arg Lys Val Leu Leu Cys Glu Arg
20 25 30
Gln Glu Arg Val Gly Gly Asn Ile Thr Thr Gly Lys Ala Gly Gly Phe
35 40 45
Leu Trp Glu Glu Gly Pro Thr Ser Phe Thr Pro Thr Pro Ala Leu Leu
50 55 60
Lys Leu Ala Val Asp Val Gly Leu Arg Glu Glu Leu Val Leu Ala Asp
65 70 75 80
His Arg Leu Pro Arg Phe Val Tyr Trp Lys Gly Gln Leu Leu Pro Val
85 90 95
Pro Met Ser Pro Pro Ser Ala Val Thr Ser Lys Leu Leu Ser Leu Ser
100 105 110
Gly Lys Phe Arg Ala Leu Val Gly Ala Leu Gly Phe Ile Pro Pro Ala
115 120 125
Ile Gly Asn His Leu Ser Gln Gln Gly Gly Glu Glu Thr Val Ala Gln
130 135 140
Phe Phe Lys Arg His Leu Gly Thr Glu Val Ala Glu Arg Leu Val Ala
145 150 155 160
Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Val His Gln Leu Ser Ala
165 170 175
Arg Ser Ala Phe Arg Arg Ile Ala Gln Leu Glu Asn Val Gly Gly Gly
180 185 190
Leu Val Ser Gly Ala Ile Leu Ser Arg Lys Gln Arg Gln Gln Gln Lys
195 200 205
Pro Pro Thr Asp Pro Ser Leu Pro Thr Val Arg Arg Gly Glu Leu Gly
210 215 220
Ser Phe Lys Glu Gly Leu Gln Ser Leu Pro Lys Ala Ile Ala Ser His
225 230 235 240
Leu Gly Glu Asn Ile Lys Leu Asn Trp Thr Leu Thr Glu Leu Arg Gln
245 250 255
Thr Ala Asn Gln Thr Tyr Ile Ala Glu Phe Ser Thr Pro Glu Gly Ser
260 265 270
Gln Gln Val Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr Val
275 280 285
Thr Ala Glu Leu Leu His Asn Leu Ala Pro Asn Ala Ser Ile Ala Leu
290 295 300
Lys Glu Ile Pro Tyr Pro Ser Val Ala Cys Val Val Leu Ala Tyr Pro
305 310 315 320
Asp Asp Ala Leu Lys Phe Pro Leu Lys Gly Phe Gly Asn Leu Ile Pro
325 330 335
Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu
340 345 350
Phe Pro Gly Arg Ala Pro Gln Gly Trp Gln Met Leu Thr Asn Phe Ile
355 360 365
Gly Gly Ala Thr Asp Pro Glu Val Gly Asn Leu Asp Asn Glu Gln Leu
370 375 380
Val Gln Ala Val His Lys Asp Leu Gln Arg Val Leu Leu Lys Lys Asp
385 390 395 400
Val Pro Pro Lys Ala Ile Ala Val His Leu Trp Lys Arg Ala Ile Pro
405 410 415
Gln Tyr Thr Leu Gly His His Leu Arg Leu Ala Gln Ile Asn Gln Asp
420 425 430
Leu Ala Gln Leu Pro Gly Leu Tyr Leu Cys Ser Asn Tyr Thr Asp Gly
435 440 445
Val Ser Leu Gly Asp Cys Val Gln Arg Ala Tyr Asp Gln Leu Pro Ile
450 455 460
Ile Asn Lys Gln Leu Ser Ile Ile Asn Asp Asn
465 470 475
<210> 13
<211> 487
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAE70643)
<400> 13
Met Glu Val Leu Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu
1 5 10 15
Ser Leu Ala His Ala Leu Gln Lys Glu Ala Thr Ser Ala Ser Pro Arg
20 25 30
Ala Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Ala Thr Gly Glu Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Phe Val Tyr Trp
85 90 95
Glu Lys Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile Gln
100 105 110
Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly Ala
115 120 125
Leu Gly Phe Val Gly Pro Ala Met Gly Ser Gln Leu Ser Gln Gln Gly
130 135 140
Asp Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Lys Glu
145 150 155 160
Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr Lys
180 185 190
Met Ala Asp Ala Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala
195 200 205
Arg Lys Arg Pro Pro Gln Pro Pro Leu Thr Lys Gly Gly Leu Lys Thr
210 215 220
Val Ala Asp Pro Asn Ile Pro Lys Thr Lys Pro Gly Glu Leu Gly Ser
225 230 235 240
Phe Lys Gly Gly Leu Gln Ala Leu Pro Glu Ala Ile Ala Ala Asn Leu
245 250 255
Gly Asp Arg Leu Lys Leu Asn Trp His Leu Thr Arg Leu Asp Arg Thr
260 265 270
Glu Arg Asp Thr Tyr Ile Ala Val Phe Ser Thr Pro Asp Gly Gln Gln
275 280 285
Glu Val Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr Val Thr
290 295 300
Ala Glu Leu Leu Glu Pro Leu Gln Pro Ser Val Ser Ser Ala Leu Gln
305 310 315 320
Ala Phe Thr Tyr Pro Thr Val Ala Ser Val Val Leu Ala Tyr Pro Met
325 330 335
Ser Asp Leu Lys Gly Lys Leu Val Gly Phe Gly Asn Leu Ile Pro Arg
340 345 350
Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe
355 360 365
Ala Asp Arg Ala Pro Ala Gly Trp Gln Thr Leu Thr Ser Tyr Ile Gly
370 375 380
Gly Ala Thr Asp Ser Gly Ile Gly Asn Leu Asp Ala Glu Gln Ile Val
385 390 395 400
Gly Glu Val His Arg Asp Leu Ser Gln Ile Leu Leu Lys Pro Glu Ala
405 410 415
Ala Gln Pro Lys Val Leu Thr Val Lys Ile Trp Lys Arg Ala Ile Pro
420 425 430
Gln Tyr Asn Leu Gly His Phe Asp Arg Leu Gln Gln Ile Asp Arg Gly
435 440 445
Leu Lys Ser Leu Pro Gly Leu Tyr Leu Cys Thr Asn Tyr Phe Gly Gly
450 455 460
Val Ala Leu Gly Asp Cys Val Arg Arg Gly Phe Glu Arg Ala Gln Glu
465 470 475 480
Val Asp Glu Tyr Leu Asn Gly
485
<210> 14
<211> 487
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAE14532)
<400> 14
Met Glu Val Leu Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu
1 5 10 15
Ser Leu Ala His Ala Leu Gln Lys Glu Ala Arg Ser Ala Ser Pro Arg
20 25 30
Ala Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Ala Thr Gly Glu Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Phe Val Tyr Trp
85 90 95
Glu Lys Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile Gln
100 105 110
Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly Ala
115 120 125
Leu Gly Phe Val Gly Pro Ala Met Gly Ser Gln Leu Ser Gln Gln Gly
130 135 140
Asp Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Lys Glu
145 150 155 160
Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr Lys
180 185 190
Met Ala Asp Ala Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala
195 200 205
Arg Lys Arg Pro Pro Gln Pro Pro Leu Thr Lys Gly Gly Leu Lys Thr
210 215 220
Val Ala Asp Pro Asn Ile Pro Lys Thr Lys Pro Gly Glu Leu Gly Ser
225 230 235 240
Phe Lys Gly Gly Leu Lys Ala Leu Pro Glu Ala Ile Ala Ala Asn Leu
245 250 255
Gly Asp Arg Leu Lys Leu Asn Trp His Leu Thr Arg Leu Asp Arg Thr
260 265 270
Glu Arg Asp Thr Tyr Ile Ala Val Phe Ser Thr Pro Asp Gly Gln Gln
275 280 285
Glu Val Glu Ala Arg Thr Val Val Leu Thr Thr Pro Ala Tyr Val Thr
290 295 300
Ala Glu Leu Leu Glu Pro Leu Gln Pro Ser Val Ser Ser Ala Leu Gln
305 310 315 320
Ala Phe Thr Tyr Pro Thr Val Ala Ser Val Val Leu Ala Tyr Pro Met
325 330 335
Ser Asp Leu Lys Gly Lys Leu Val Gly Phe Gly Asn Leu Ile Pro Arg
340 345 350
Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe
355 360 365
Ala Asp Arg Ala Pro Ala Gly Trp Gln Thr Leu Thr Ser Tyr Ile Gly
370 375 380
Gly Ala Thr Asp Ser Gly Ile Gly Asn Leu Asp Ala Glu Gln Ile Val
385 390 395 400
Gly Glu Val His Arg Asp Leu Ser Gln Ile Leu Leu Lys Pro Glu Ala
405 410 415
Ala Gln Pro Lys Val Leu Thr Val Lys Ile Trp Lys Arg Ala Ile Pro
420 425 430
Gln Tyr Asn Leu Gly His Phe Asp Arg Leu Glu Gln Ile Asn Arg Gly
435 440 445
Leu Lys Ser Leu Pro Gly Leu Tyr Leu Cys Thr Asn Tyr Phe Gly Gly
450 455 460
Val Ala Leu Gly Asp Cys Val Arg Arg Gly Phe Glu Arg Ala Gln Glu
465 470 475 480
Val Asp Glu Tyr Leu Asn Gly
485
<210> 15
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanobacteria bacterium QS_8_64_29 (PSP16006)
<400> 15
Met Leu Asp Ala Leu Val Val Gly Ala Gly Ile Ser Gly Leu Ser Leu
1 5 10 15
Gly Arg Thr Leu Gln Gln Gln Gln His Ser Leu Leu Val Ala Glu Arg
20 25 30
Gln Pro Gln Val Gly Gly Asn Ile Thr Thr Ser Ser Thr Gly Glu Phe
35 40 45
Leu Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro Ala Arg Glu Leu Leu
50 55 60
Glu Leu Ala Val Asp Ala Gly Leu Arg Glu Glu Leu Val Leu Ala Asp
65 70 75 80
Arg Gln Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Arg Leu Gln Pro Val
85 90 95
Pro Met Arg Pro Ser Ala Ala Leu Lys Thr Gln Leu Leu Ser Pro Ser
100 105 110
Gly Lys Leu Arg Ala Leu Ala Gly Ala Leu Gly Phe Val Pro Pro Ala
115 120 125
Val Gly Glu Ala Val Ser Gln Gln Gly Gly Glu Glu Thr Val Ala Gln
130 135 140
Phe Phe Gln Arg His Leu Gly Arg Glu Val Thr Glu Arg Leu Val Ala
145 150 155 160
Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Val Arg Gln Leu Ser Ala
165 170 175
Gln Ala Ala Phe Arg Arg Val Thr Gln Leu Ala Asp Arg Gly Gly Gly
180 185 190
Leu Leu Pro Gly Ala Leu Leu Gly Arg Gln Ala Ala Pro Gln Arg Pro
195 200 205
Ala Thr Ser Gln Thr Gln Leu Pro Gln Thr Gln Ser Gly Glu Leu Gly
210 215 220
Ser Phe Arg Thr Gly Leu Gln Ala Leu Pro Asn Ala Ile Ala Glu Arg
225 230 235 240
Leu Gly Asp Ala Leu Arg Cys Asn Trp Ala Leu Ser Gln Leu Gln Arg
245 250 255
Thr Glu Arg Asp Ser Tyr Leu Ala Thr Phe Gln Thr Pro Gln Gly Pro
260 265 270
Gln Gln Val Glu Ala Arg Ala Val Val Leu Thr Thr Pro Ala Tyr Thr
275 280 285
Ser Ala Glu Leu Leu Arg Pro Leu His Ala Arg Ala Ser Gln Arg Leu
290 295 300
Ala Ala Ile Pro Tyr Pro Pro Val Ala Ser Val Val Leu Ala Tyr Pro
305 310 315 320
Gln Gln Ala Leu Gly Gly Ala Leu Arg Gly Phe Gly Asn Leu Asn Pro
325 330 335
Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ala Leu
340 345 350
Phe Ala Gly Arg Ala Pro Ala Gly Trp Glu Ile Leu Thr Ser Phe Ile
355 360 365
Gly Gly Ala Thr Asp Pro Glu Ile Ala Arg Leu Asp Glu Asp Arg Ile
370 375 380
Val Gln Ala Val His Ala Asp Leu Arg Gln Val Leu Leu Ala Arg Tyr
385 390 395 400
Val Ala Pro Lys Val Leu Ala Val Arg Leu Trp His Arg Ala Ile Pro
405 410 415
Gln Tyr Ala Ile Gly His Gln Ala Gln Met Gln Ala Leu Glu Ala Glu
420 425 430
Leu Ala Glu Leu Pro Gly Leu Phe Leu Cys Ser Asn Tyr Leu Asp Gly
435 440 445
Val Ser Leu Gly Asp Cys Ile Arg Arg Gly Phe Glu Arg Ala His Thr
450 455 460
Val Ser His Tyr Leu Gln Ala Pro Ala Ala Ala Pro Ala Ala
465 470 475
<210> 16
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Lyngbya aestuarii (WP_023067908)
<400> 16
Met Thr His Val Leu Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ser Leu Leu Arg Asn Pro Asn Pro Gln Leu Ser
20 25 30
Leu Asn Ile Leu Val Ser Glu His Gln Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Val Ser Gln Gly Glu Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Glu Val Gly Leu
65 70 75 80
Lys Ser Glu Phe Val Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Asn Ser Gln Leu Met Pro Val Pro Met Ser Pro Pro Ala Leu Leu
100 105 110
Ser Thr Lys Leu Leu Ser Pro Gly Gly Lys Leu Arg Ala Leu Thr Gly
115 120 125
Ala Leu Gly Phe Val Arg Pro Ala Met Gly Gln Ala Leu Ser Gln Gln
130 135 140
Asn Gly Glu Glu Thr Ile Ser Gln Phe Phe Glu Arg His Leu Gly Ser
145 150 155 160
Glu Val Leu Lys Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Glu Ile Ser Ser Ala Phe Ala Arg Val Ala
180 185 190
Arg Met Ala Tyr Ser Gly Gly Gly Leu Val Ala Gly Ala Val Leu Ser
195 200 205
Arg Gly Gln Asn Lys Ser Ser Arg Ser Pro Ala Asp Pro Ser Ile Pro
210 215 220
Gln Thr Lys Arg Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Gly Ala
225 230 235 240
Leu Pro Asn Ala Ile Ala Gln Gln Leu Gly Asp Gln Leu Lys Leu Asn
245 250 255
Trp Gln Leu Thr Arg Leu Glu Arg Thr Glu Asn Gln Thr Tyr Arg Ala
260 265 270
Glu Phe Ser Thr Pro Asp Gly Val Gln Gln Val Glu Thr Arg Thr Val
275 280 285
Val Leu Thr Thr Pro Ala Tyr Val Thr Ala Glu Ile Leu Lys Pro Leu
290 295 300
Gln Leu Gln Val Ser Gln Thr Leu Thr Glu Ile Pro Tyr Pro Pro Val
305 310 315 320
Ala Cys Val Val Leu Ala Tyr Pro Val Ser Ala Phe Lys Gln Lys Leu
325 330 335
Thr Gly Phe Gly Asn Leu Val Pro Arg Gly Gln Gly Ile Arg Thr Leu
340 345 350
Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro Gln Gly
355 360 365
Trp Gln Val Leu Thr Ser Tyr Ile Gly Gly Ala Thr Asp Pro Glu Ile
370 375 380
Gly Glu Leu Glu Asp Asp Gln Ile Val Glu Ala Val His Gln Asp Leu
385 390 395 400
Arg Arg Ile Leu Leu Lys Glu Asp Ile Ser Pro Lys Val Leu Ala Val
405 410 415
His Leu Trp Lys Arg Ala Ile Pro Gln Tyr Asn Leu Gly His Gln Gln
420 425 430
Arg Leu Gln Gln Val Asn Glu Gly Leu Glu Ala Met Pro Gly Leu Tyr
435 440 445
Leu Cys Ser Asn Tyr Ile Asp Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Arg Ser Ile Gly Gln Ala Asn Glu Ile Leu Ser Phe Leu Asp Gln
465 470 475
<210> 17
<211> 481
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Tychonema bourrellyi (WP_096831359)
<400> 17
Met Thr Asn Val Val Asp Ser Leu Ile Val Gly Ala Gly Ile Thr Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu His Lys Glu Ala Lys Thr Gly Thr Pro
20 25 30
Val Lys Ile Leu Val Ala Glu Ser Leu Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Cys Thr Gly Asn Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ser Pro Thr Val Glu Leu Met Lys Leu Ala Val Asp Val Gly Leu
65 70 75 80
Lys Glu Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Phe Val Tyr
85 90 95
Trp Gln Asn Lys Leu Gln Pro Val Pro Met Thr Pro Gln Ala Met Ile
100 105 110
Gln Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly
115 120 125
Ala Leu Gly Phe Val Ala Pro Ala Met Gly Ala Thr Leu Ser Gln Gln
130 135 140
Gly Gly Gln Glu Thr Ile Ser Gln Phe Phe Arg Arg His Leu Gly Thr
145 150 155 160
Glu Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Glu Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr
180 185 190
Arg Met Ala Asp Leu Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Gly
195 200 205
Ala Arg Lys Gly Pro Lys Lys Met Pro Ala Asp Pro Asn Ile Pro Lys
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Lys Gly Gly Leu Lys Ala Leu
225 230 235 240
Pro Glu Ala Ile Ala Ala Gln Leu Gly Asp Arg Leu Lys Leu Asn Trp
245 250 255
His Leu Thr Gly Leu His Arg Thr Glu Asn Lys Thr Tyr Ile Ala Glu
260 265 270
Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Thr Arg Thr Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Thr Ala Asp Trp Phe Gln Ser Leu Gln
290 295 300
Pro Glu Val Ser Thr Ala Leu Gln Ala Phe Thr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Lys Ser Asp Val Lys Glu Lys Met Val
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Ser Ser Ser Leu Phe Ala Asn Arg Ala Pro Ala Gly Trp
355 360 365
Gln Thr Leu Thr Ser Phe Ile Gly Gly Ala Thr Asp Ser Gly Ile Ala
370 375 380
Asn Leu Asp Ala Glu Gln Ile Val Ala Gln Val His Arg Asp Leu Ser
385 390 395 400
Arg Ile Leu Leu Lys Pro Asp Val Pro Gln Pro Lys Val Leu Ala Val
405 410 415
Lys Val Trp Lys Gln Ala Ile Pro Gln Tyr Asn Leu Gly His Phe Asp
420 425 430
Arg Leu Glu Gln Ile Asp Arg Gly Leu Lys Ser Leu Pro Gly Val Tyr
435 440 445
Leu Cys Ser Asn Tyr Leu Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Leu Gly Leu Glu Arg Ala Ile Glu Val Ser Lys Tyr Leu Gln Glu Thr
465 470 475 480
Ser
<210> 18
<211> 481
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAG91209)
<400> 18
Met Thr Asn Val Val Asp Thr Leu Ile Val Gly Ala Gly Ile Thr Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu Gln Lys Glu Ala Lys Thr Gly Thr Pro
20 25 30
Val Lys Ile Leu Val Ala Glu Ser Leu Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Cys Thr Gly Asn Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ser Pro Thr Ala Glu Leu Met Lys Leu Ala Val Asp Val Gly Leu
65 70 75 80
Lys Glu Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Phe Val Tyr
85 90 95
Trp Gln Asn Lys Leu Gln Pro Val Pro Met Thr Pro Gln Ala Met Ile
100 105 110
Gln Ser Gln Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly
115 120 125
Ala Leu Gly Phe Val Ala Pro Ala Met Gly Ala Thr Leu Ser Gln Gln
130 135 140
Gly Gly Gln Glu Thr Val Ser Gln Phe Phe Gly Arg His Leu Gly Thr
145 150 155 160
Glu Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr
180 185 190
Arg Met Ala Asp Leu Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Gly
195 200 205
Ala Arg Lys Ala Pro Lys Lys Met Pro Ala Asp Pro Asn Val Pro Lys
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Lys Gly Gly Leu Lys Ala Leu
225 230 235 240
Pro Glu Ala Ile Ala Ala Gln Leu Gly Asp Arg Leu Lys Leu Asn Trp
245 250 255
His Leu Thr Gly Leu His Arg Thr Glu Asn Lys Thr Tyr Ile Ala Glu
260 265 270
Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Thr Arg Thr Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Thr Ala Asp Leu Phe Gln Ser Leu Gln
290 295 300
Pro Glu Val Ser Ser Ala Leu Gln Ala Phe Thr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Lys Ser Asp Val Lys Glu Lys Met Val
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Thr Ser Ser Leu Phe Ala Asn Arg Ala Pro Ala Gly Trp
355 360 365
Gln Thr Leu Thr Ser Tyr Ile Gly Gly Ala Thr Asp Ser Gly Ile Gly
370 375 380
Asn Leu Asp Ala Glu Gln Ile Val Gly Glu Val His Arg Asp Leu Ser
385 390 395 400
Arg Ile Leu Leu Lys Pro Asn Val Pro Gln Pro Lys Val Leu Ala Val
405 410 415
Lys Val Trp Lys Gln Ala Ile Pro Gln Tyr Asn Leu Gly His Phe Asp
420 425 430
Arg Leu Glu Arg Ile Asp Arg Gly Leu Lys Ser Leu Pro Gly Val Tyr
435 440 445
Leu Cys Ser Asn Tyr Val Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Leu Gly Phe Glu Arg Ala Ile Glu Val Ser Glu Tyr Leu Gln Ala Thr
465 470 475 480
Ser
<210> 19
<211> 466
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanobacteria bacterium SW_9_44_58 (PSO49761)
<400> 19
Met Leu Asp Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu Ser Thr
1 5 10 15
Ala Tyr Arg Leu His Gln Gly Gln Arg Gln Ile Leu Val Ala Glu Gln
20 25 30
Arg Asp Arg Val Gly Gly Asn Ile Ala Thr Glu His Gln Gly Glu Phe
35 40 45
Leu Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro Thr Pro Glu Leu Leu
50 55 60
Lys Leu Ala Val Asp Ala Gly Leu Lys Asn Asp Phe Val Phe Ala Asp
65 70 75 80
Arg Asn Leu Pro Arg Tyr Val Tyr Trp Gln Gly Lys Leu Arg Pro Val
85 90 95
Pro Met Ser Pro Pro Ala Ala Val Lys Ser Gln Leu Leu Ser Pro Trp
100 105 110
Gly Lys Leu Arg Ala Leu Ala Gly Ala Leu Gly Phe Val Ser Pro Asn
115 120 125
Val Glu Gly Lys Glu Glu Thr Val Ala Asp Phe Phe Thr Arg His Leu
130 135 140
Gly Glu Glu Val Ala Gln Arg Leu Val Ala Pro Phe Val Ser Gly Val
145 150 155 160
Tyr Ala Gly Asp Val His Arg Leu Ser Ala Gln Ala Ala Phe Gly Arg
165 170 175
Val Thr Gln Leu Ala Asp Val Gly Gly Gly Leu Val Ala Gly Ala Val
180 185 190
Leu Ser Arg Gly Lys Arg Lys Gln Ser Ser Ser Thr Val Thr Glu Asn
195 200 205
Ala Asp Ile Pro Lys Thr Lys Ser Gly Glu Leu Gly Ser Phe Arg Glu
210 215 220
Gly Leu Gln Met Leu Pro Arg Ala Ile Ala Ser Lys Leu Gly Glu Ser
225 230 235 240
Val Lys Leu Asn Trp Gln Leu Asn Asn Ile Ser Pro His Pro Glu Gln
245 250 255
Gly Tyr Ile Ala Ala Phe Ser Thr Pro Glu Gly Glu Gln Ser Val Glu
260 265 270
Ala Lys Ser Ile Val Leu Thr Thr Pro Ala His Val Thr Ala Pro Ile
275 280 285
Ile Gln Thr Leu Ser Pro Leu Thr Ser Thr Ala Leu Gln Asp Ile Ser
290 295 300
Tyr Pro Pro Val Ala Cys Val Ile Leu Ala Tyr Pro Asp Glu Ala Leu
305 310 315 320
Arg Phe Ser Leu Lys Gly Phe Gly Asn Leu Val Pro Arg Asn Gln Gly
325 330 335
Leu Arg Thr Leu Gly Thr Ile Trp Ala Ser Thr Leu Phe Pro Gly Arg
340 345 350
Ala Pro Gln Gly Trp His Ile Leu Thr Asn Phe Ile Gly Gly Ala Thr
355 360 365
Asp Pro Glu Ile Ala Gln Leu Ser Glu Glu Gln Ile Ile Asp Gln Val
370 375 380
His Gln Asp Leu Gln Lys Val Leu Leu Lys Ser Asp Thr Asn Pro Lys
385 390 395 400
Pro Leu Ala Val His Leu Trp Ser Lys Ala Ile Pro Gln Tyr Thr Leu
405 410 415
Gly His Leu Asp Arg Leu Glu Thr Ile Arg Asn Ser Leu Lys Ser Cys
420 425 430
Pro Gly Leu Phe Leu Cys Ser Asn Tyr Leu Asp Gly Val Ser Leu Gly
435 440 445
Asp Cys Val Arg Arg Gly Glu Glu Thr Ala Gln Ala Val Leu Asp Tyr
450 455 460
Leu Gly
465
<210> 20
<211> 482
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Trichodesmium erythraeum (WP_011611816)
<400> 20
Met Thr Glu Ile Leu Asp Val Leu Val Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Lys Leu Thr Lys Leu Ser Asn Asn Ser Pro Leu
20 25 30
Lys Ile Leu Val Ala Glu Ser Gln Asn Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Val Ser Gln Gly Glu Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Leu Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Glu Asp Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp
85 90 95
Asn Gly Gln Leu Leu Pro Val Pro Met Ser Pro Lys Ala Met Leu Gln
100 105 110
Ser Gln Leu Leu Ser Asn Thr Gly Lys Leu Arg Ala Leu Val Gly Ala
115 120 125
Leu Gly Phe Val Pro Pro Val Val Gly Met Asp Leu Ser Gln Glu Gly
130 135 140
Gly Glu Glu Thr Val Ser Gln Phe Phe Gln Arg His Leu Gly Lys Glu
145 150 155 160
Val Met Glu Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Ser Gln Leu Ser Ala Thr Ala Ala Phe Ser Lys Val Ala Arg
180 185 190
Met Ala Asp Leu Gly Gly Gly Leu Leu Ala Gly Ala Val Leu Ser Ala
195 200 205
Gln Arg Asn Pro Lys Ser Lys Ala Ala Ala Asn Ser Asn Ile Pro Lys
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Arg Arg Gly Leu Glu Val Leu
225 230 235 240
Pro Lys Ala Ile Ala Thr Tyr Leu Gly Gln Ala Val Lys Leu Asn Trp
245 250 255
Ser Leu Val Gly Leu Arg Pro Thr Glu Lys Gln Thr Tyr Ile Ala Glu
260 265 270
Phe Ser Thr Pro Asn Gly Ser Gln Gln Ile Glu Thr Arg Thr Ile Ala
275 280 285
Leu Ser Ser Pro Ala Tyr Ala Cys Ala Lys Leu Phe Arg Pro Leu Leu
290 295 300
Pro Glu Ile Ala Gly Thr Leu Asp Glu Phe Tyr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Val Ser Ser Ile Lys Ala Lys Ile Asp
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Ser Ser Ala Leu Phe Ser Gly Arg Thr Pro Leu Gly Trp
355 360 365
Gln Ile Phe Thr Asn Tyr Ile Gly Gly Ala Thr Asp Pro Glu Ile Ser
370 375 380
His Leu Asp Ser Glu Ala Ile Val Ala Gln Val His Gln Asp Leu Cys
385 390 395 400
Gln Thr Leu Leu Asn Gln Asn Pro Glu Lys Pro Lys Val Leu Ala Val
405 410 415
His Ile Trp Ser Arg Ala Ile Pro Gln Tyr Asn Leu Gly Tyr Ser Ser
420 425 430
Arg Leu Ala Gln Ile Asn His Gly Leu Lys Ser Trp Pro Gly Val Tyr
435 440 445
Leu Cys Ser Asn Tyr Ile Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Arg Ser Ile Glu Val Ala Thr Asp Ile Tyr Ser Gly Met Gly Phe Gly
465 470 475 480
Glu Leu
<210> 21
<211> 498
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Geitlerinema sp. PCC 9228 (WP_071516524.1)
<400> 21
Met Asn Ala Ile Ala Glu Asn Gly Asn Glu Ala Asn Glu Ala Ser Pro
1 5 10 15
Val Trp Val Asp Thr Leu Ile Val Gly Gly Gly Ile Ser Gly Leu Ser
20 25 30
Val Ala His Ala Leu Val Asn Cys Asp Arg Ser Val Asp Asn Ile Leu
35 40 45
Leu Ala Glu Ser Gln Pro Arg Val Gly Gly Ser Val Thr Ser Ala Ser
50 55 60
Ser Glu Gly Phe Leu Tyr Glu Glu Gly Pro Asn Ser Phe Ser Pro Thr
65 70 75 80
Pro Glu Leu Leu Gln Leu Ala Val Asp Val Gly Leu Lys Asp Glu Leu
85 90 95
Ile Leu Ala Asp Arg Arg Leu Pro Arg Tyr Val Tyr Trp Gln Gly Arg
100 105 110
Leu Ile Ala Leu Pro Asn Ser Pro Pro Ser Ala Val Thr Ser Pro Ile
115 120 125
Leu Ser Pro Val Gly Lys Leu Arg Ala Leu Leu Gly Ala Leu Gly Phe
130 135 140
Val Pro Pro His Val Thr Ser Glu Pro Glu Ser Val Ser Asp Phe Ile
145 150 155 160
Arg Arg His Leu Gly Pro Glu Val Leu Gln Lys Leu Val Glu Pro Phe
165 170 175
Thr Ser Gly Val Tyr Ala Gly Asn Pro Asp Glu Leu Glu Ala Ala Ser
180 185 190
Ala Phe Ala Arg Ile Ala Arg Leu Glu Lys Val Gly Gly Ser Leu Val
195 200 205
Ala Gly Ala Ile Leu Ser Arg Arg Gln Ala Pro Thr Gln Lys Lys Asn
210 215 220
Arg Asp Arg Asn Leu Pro Lys Thr Gln Arg Gly Gln Leu Gly Ser Phe
225 230 235 240
Gln Arg Gly Leu Gln Ser Leu Pro Glu Ala Ile Ala Gly Lys Leu Gly
245 250 255
Ser Arg Val Arg Val Asn Trp Gln Ala Lys Ser Ile Gly Lys Thr Glu
260 265 270
Gly Gly Asn Tyr Leu Val Glu Phe Ala Thr Pro Gln Gly His Gln Gln
275 280 285
Val Glu Ala Arg Ser Leu Val Leu Ala Thr Pro Ala Tyr Val Thr Ala
290 295 300
Asn Leu Leu Lys Ser Tyr Pro His Val Asn Ser Gln Gln Gln Gln Ala
305 310 315 320
Ile Gln Ala Leu Glu Ser Ile Pro Tyr Pro Pro Val Ala Cys Val Val
325 330 335
Leu Gly Tyr Pro Ser Ser Ala Phe Lys Lys Gln Pro Leu His Gly Phe
340 345 350
Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile
355 360 365
Trp Gly Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu Gly Trp Glu Leu
370 375 380
Leu Leu Asn Phe Ile Gly Gly Thr Thr Asp Pro Glu Ile Ala Asn Leu
385 390 395 400
Asp Gln Glu Gln Ile Ala Gln Leu Val His Arg Asp Leu Cys Gln Thr
405 410 415
Leu Leu Arg Glu Asp Lys Gln Pro Lys Val Leu Gln Val His Leu Trp
420 425 430
Lys Gln Ala Ile Pro Gln Tyr Thr Ile Gly His Gly Ser Arg Leu Ala
435 440 445
Ala Ile Asp Ala Gly Val Arg Ser Leu Pro Gly Leu Phe Leu Cys Ser
450 455 460
Asn Tyr Ser Asp Gly Val Ala Met Gly Asp Cys Ala Arg Arg Gly Tyr
465 470 475 480
Glu Leu Ala Pro Arg Val Ala Glu Tyr Leu Gln Pro Gly Ser Tyr Lys
485 490 495
Phe Val
<210> 22
<211> 496
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAD79992.1)
<400> 22
Met Val Val Ala Thr Pro Gly Ala Val Glu Ser Ser Val Ala Arg Ser
1 5 10 15
Asn Pro Ile Asp Leu Leu Ile Val Gly Ala Gly Leu Thr Gly Leu Ser
20 25 30
Ala Ala Gln Arg Phe Val Arg Gln Ser Pro Gly Arg Ser Cys Leu Val
35 40 45
Val Glu Ala Gln Asp Arg Val Gly Gly Asn Ile Thr Thr Arg Ser Gly
50 55 60
Asn Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe Ala Pro Thr Pro
65 70 75 80
Glu Leu Leu Gln Leu Ala Val Glu Val Gly Leu Lys Asp Gln Leu Val
85 90 95
Phe Ala Asp Gly Lys Leu Pro Arg Phe Val Tyr Trp Asp Gly Arg Leu
100 105 110
Gln Ala Ile Pro Met Ser Pro Gly Ala Phe Trp Asn Ser Thr Leu Leu
115 120 125
Ser Asp Arg Gly Lys Ala Arg Leu Leu Leu Gly Ala Ala Gly Phe Val
130 135 140
Pro Pro Ile Leu Gly Ala Thr Val Gln Ala Arg Gly Gly Glu Glu Thr
145 150 155 160
Val Arg Glu Phe Phe Thr Arg His Leu Gly Gln Glu Thr Met Glu Arg
165 170 175
Met Val Asp Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp Pro Asp Ala
180 185 190
Leu Ser Ala Ser Ala Ala Phe Arg Lys Met Ala Ala Met Gln Ala Ala
195 200 205
Gly Gly Gly Leu Ala Ala Gly Ala Ile Arg Thr Leu Leu Ala Lys Arg
210 215 220
Arg Ala Ala Lys Thr Ala Pro Pro Ala Asp Pro Asn Leu Pro Lys Pro
225 230 235 240
Lys Ser Gly Glu Leu Gly Ser Phe Arg Glu Gly Leu Gln Met Leu Pro
245 250 255
Glu Ala Val Ala Lys Glu Leu Gly Asp Arg Val Lys Leu Gly Trp Arg
260 265 270
Val Glu Ala Ile Ala Arg Ser Ser Asn Gly Asp Tyr Thr Val Asp Leu
275 280 285
Ala Thr Pro Asp Gly Pro Arg Gln Ile Thr Thr Arg Ala Ile Val Leu
290 295 300
Ala Thr Pro Ala Pro Ala Thr Ala Asn Leu Leu Gln Pro Leu Ala Pro
305 310 315 320
Ala Ala Ser Ala Ala Leu His Ala Ile Pro Tyr Pro Ala Val Ala Cys
325 330 335
Val Ile Leu Ala Tyr Pro Glu Thr Ala Phe Ala Gln Ser Leu Arg Gly
340 345 350
Phe Gly Asn Leu Ile Pro Arg Ser Leu Gly Leu Gln Thr Leu Gly Thr
355 360 365
Ile Trp Ala Ser Ser Leu Phe Ala Gly Arg Ala Pro Gln Gly Trp Ala
370 375 380
His Leu Ile Asn Phe Ile Gly Gly Ala Gln Asn Pro Gly Leu Ile Asn
385 390 395 400
Lys Thr Glu Ala Glu Ile Ala Asp Ile Val His Gly Asp Val Arg Arg
405 410 415
Ile Leu Leu Lys Glu Asp Val Ala Pro Lys Val Leu Ser Val Lys Leu
420 425 430
Trp Arg Arg Ala Ile Pro Gln Tyr Thr Ile Gly His Ala Asp Arg Leu
435 440 445
Ala Thr Leu His Arg Glu Leu Ala Ala Trp Pro Gly Leu Phe Pro Cys
450 455 460
Ser Asn Tyr Glu Gly Gly Val Ala Leu Gly Asp Cys Val Arg His Gly
465 470 475 480
Trp Asp Gln Ala Ala Glu Val Asp Arg Tyr Leu Ala Gln Leu Pro Val
485 490 495
<210> 23
<211> 481
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAD82603.1)
<400> 23
Met Thr Asn Arg Val Asp Thr Leu Ile Val Gly Ala Gly Ile Thr Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu His Lys Glu Ala Lys Thr Gly Glu Pro
20 25 30
Leu Lys Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Ser Thr Gly His Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ser Pro Thr Pro Glu Leu Met Lys Leu Ala Val Asp Val Gly Leu
65 70 75 80
Lys Gln Glu Leu Met Phe Ala Asp Arg Gln Leu Pro Arg Phe Val Tyr
85 90 95
Trp Gln Asn Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile
100 105 110
Gln Ser Gly Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly
115 120 125
Ala Leu Gly Phe Val Ala Pro Val Met Gly Ala Thr Leu Ser Gln Gln
130 135 140
Gly Glu Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Thr
145 150 155 160
Glu Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr
180 185 190
Lys Met Ala Asp Ala Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser
195 200 205
Ala Arg Lys Arg Pro Lys Lys Met Pro Pro Asp Pro Asn Ile Pro Gln
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Lys Gly Gly Leu Met Ala Leu
225 230 235 240
Pro Glu Ala Ile Ala Ala Ser Leu Gly Asp Arg Leu Lys Leu Asn Trp
245 250 255
His Leu Thr Gly Leu His Arg Thr Glu Asn Lys Thr Tyr Ile Ala Glu
260 265 270
Phe Ser Thr Pro His Gly Pro Gln Gln Ile Glu Thr Arg Thr Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Ala Ala Asp Leu Leu Gln Ser Leu Gln
290 295 300
Pro Glu Val Ser Ser Thr Leu Gln Gly Phe Thr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Leu Ser Asp Val Lys Gly Lys Leu Val
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Thr Ser Ser Leu Phe Ala Asp Arg Ala Pro Ala Gly Trp
355 360 365
Gln Thr Leu Thr Ser Tyr Ile Gly Gly Ala Thr Asp Ser Gly Ile Gly
370 375 380
Asn Leu Asp Pro Glu Glu Ile Val Ala Glu Val His Arg Asp Leu Ser
385 390 395 400
Arg Ile Leu Leu Lys Pro Asn Val Pro Gln Pro Lys Val Leu Ala Val
405 410 415
Lys Leu Trp Lys Gln Ala Ile Pro Gln Tyr Asn Leu Gly His Leu Asp
420 425 430
Arg Leu Gln Gln Ile Asp Arg Gly Leu Lys Ser Leu Pro Gly Met Tyr
435 440 445
Leu Cys Ser Asn Tyr Val Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Leu Gly Leu Glu Lys Ala Ile Ala Val Ser Lys Tyr Leu Gln Glu Thr
465 470 475 480
Ser
<210> 24
<211> 481
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium (TAD95528.1)
<400> 24
Met Thr Asn Arg Val Asp Thr Leu Ile Val Gly Ala Gly Ile Thr Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu His Lys Glu Ala Lys Thr Gly Glu Pro
20 25 30
Leu Lys Ile Leu Val Ala Glu Ser Gln Gly Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Ser Thr Gly His Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ser Pro Thr Pro Glu Leu Met Lys Leu Ala Val Asp Val Gly Leu
65 70 75 80
Lys Gln Glu Leu Met Phe Ala Asp Arg Gln Leu Pro Arg Phe Val Tyr
85 90 95
Trp Gln Asn Lys Leu Gln Pro Val Pro Met Thr Pro Gly Ala Met Ile
100 105 110
Gln Ser Gly Leu Leu Ser Phe Pro Gly Lys Leu Arg Ala Leu Phe Gly
115 120 125
Ala Leu Gly Phe Val Ala Pro Val Met Gly Ala Thr Leu Ser Gln Gln
130 135 140
Gly Glu Glu Glu Thr Val Ser Gln Phe Phe Arg Arg His Leu Gly Thr
145 150 155 160
Glu Val Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ser Ala Ala Ala Ala Phe Gly Arg Val Thr
180 185 190
Lys Met Ala Asp Ala Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser
195 200 205
Ala Arg Lys Arg Pro Lys Lys Met Pro Pro Asp Pro Asn Ile Pro Gln
210 215 220
Thr Lys Pro Gly Glu Leu Gly Ser Phe Lys Gly Gly Leu Met Ala Leu
225 230 235 240
Pro Glu Ala Ile Ala Ala Ser Leu Gly Asp Arg Leu Lys Leu Asn Trp
245 250 255
His Leu Thr Gly Leu His Arg Thr Glu Asn Lys Thr Tyr Ile Ala Gln
260 265 270
Phe Ser Thr Pro Asp Gly Pro Gln Gln Ile Glu Thr Arg Thr Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Ala Ala Asp Leu Leu Gln Ser Leu Gln
290 295 300
Pro Glu Val Ser Ser Thr Leu Gln Gly Phe Thr Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Leu Ser Asp Val Lys Gly Lys Leu Val
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly
340 345 350
Thr Ile Trp Thr Ser Ser Leu Phe Ala Asp Arg Ala Pro Ala Gly Trp
355 360 365
Gln Thr Leu Thr Ser Tyr Ile Gly Gly Ala Thr Asp Ser Gly Ile Gly
370 375 380
Asn Leu Asp Pro Glu Glu Ile Val Ala Glu Val His Arg Asp Leu Ser
385 390 395 400
Arg Ile Leu Leu Lys Pro Asn Val Pro Gln Pro Lys Val Leu Ala Val
405 410 415
Lys Leu Trp Lys Gln Ala Ile Pro Gln Tyr Asn Leu Gly His Leu Asp
420 425 430
Arg Leu Gln Gln Ile Asp Arg Gly Leu Lys Ser Leu Pro Gly Met Tyr
435 440 445
Leu Cys Ser Asn Tyr Val Gly Gly Val Ala Leu Gly Asp Cys Val Arg
450 455 460
Leu Gly Leu Glu Lys Ala Ile Ala Val Ser Lys Tyr Leu Gln Glu Thr
465 470 475 480
Ser
<210> 25
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Limnothrix sp. PR1529 (WP_099534595.1)
<400> 25
Met Asp Val Leu Ile Val Gly Ala Gly Leu Thr Gly Leu Ser Ala Ala
1 5 10 15
His Arg Leu Val Asn His Pro Gly Asn Arg Gly Arg Ser Thr Leu Val
20 25 30
Val Glu Ala Gln Asn Arg Val Gly Gly Asn Ile Thr Thr Arg Ser Ala
35 40 45
Asp Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe Ala Pro Thr Pro
50 55 60
Glu Leu Leu Lys Leu Ala Val Glu Val Gly Leu Lys Asp Glu Leu Val
65 70 75 80
Phe Ala Asp Gly Lys Leu Pro Arg Phe Val Tyr Trp Asp Gly Arg Leu
85 90 95
Gln Ala Ile Pro Met Ser Pro Gly Ala Phe Trp Asn Ser Thr Leu Leu
100 105 110
Ser Asp Arg Gly Lys Ala Arg Leu Leu Leu Gly Ala Ala Gly Phe Val
115 120 125
Pro Pro Ile Val Gly Ala Ala Ile Ser Ala Arg Gly Gly Glu Glu Thr
130 135 140
Val Arg Glu Phe Phe Thr Arg His Leu Gly Gln Glu Ala Met Glu Arg
145 150 155 160
Leu Val Asp Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp Pro Asn Ala
165 170 175
Leu Ser Ala Ser Ala Ala Phe Arg Lys Met Ala Ala Met Gln Ala Ala
180 185 190
Gly Gly Gly Leu Gly Ala Gly Ala Ala Arg Ile Leu Trp Ala Lys Arg
195 200 205
Gln Ala Ala Lys Gln Ala Pro Pro Ala Asp Pro Arg Leu Pro Lys Pro
210 215 220
Lys Ser Gly Glu Leu Gly Ser Phe Arg Glu Gly Leu Gln Ala Leu Pro
225 230 235 240
Glu Ala Val Ala Ser Gly Leu Gly Asp Arg Val Lys Leu Gly Trp Gln
245 250 255
Val Glu Ala Ile Ala Arg Asp Pro Gln Gly Val Tyr Gln Val Ala Ile
260 265 270
Ala Ser Pro Asp Gly Pro Gln Gln Ile Thr Ala Arg Ser Ile Ile Leu
275 280 285
Ala Thr Pro Ala Pro Val Thr Ala Gln Leu Leu Glu Pro Leu Ala Pro
290 295 300
Arg Ala Ser Thr Ala Leu Asn Ala Ile Pro Tyr Pro Ala Val Ala Cys
305 310 315 320
Val Ile Leu Ala Tyr Pro Glu Thr Ala Phe Ser Gln Ser Leu Arg Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Ser Leu Gly Leu Gln Thr Leu Gly Thr
340 345 350
Ile Trp Ala Ser Ser Leu Phe Ala Gly Arg Ala Pro Gln Gly Trp Ala
355 360 365
His Leu Ile Asn Phe Ile Gly Gly Ala Gln Asn Pro Thr Leu Ile His
370 375 380
Lys Thr Glu Asp Glu Ile Ala Gln Met Val His Ala Asp Val Arg Arg
385 390 395 400
Ile Leu Leu Lys Gln Asp Val Pro Pro Lys Val Leu Ser Val Lys Leu
405 410 415
Trp Arg Arg Ala Ile Pro Gln Tyr Thr Ile Gly His Gly Ser Arg Leu
420 425 430
Ala Thr Leu His Gln Glu Leu Gln His Trp Pro Gly Leu Phe Ala Cys
435 440 445
Ser Asn Tyr Glu Gly Gly Val Ala Leu Gly Asp Cys Val Arg His Gly
450 455 460
Trp Glu Gln Ala Asp Ala Ile Glu Gln Phe Leu Gly Ser Arg Val
465 470 475
<210> 26
<211> 490
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Planktothricoides sp. SR001 (WP_054468037.1)
<400> 26
Met Asn Ser Thr Asp Leu Ala Asn Pro Ser Thr Thr Leu Asp Thr Leu
1 5 10 15
Ile Val Gly Ala Gly Ile Thr Gly Leu Ser Leu Ala Gln Ala Leu Gln
20 25 30
Gln Asp Arg Lys Arg Gly Arg Gln Ser Gly Gln Ile Leu Leu Thr Glu
35 40 45
Ser Gln Glu Arg Val Gly Gly Arg Ile Val Thr Gln Ser Gln Asp Gly
50 55 60
Phe Leu Trp Glu Glu Gly Pro Asn Ser Cys Leu Pro Thr Pro Glu Phe
65 70 75 80
Leu Lys Leu Ala Val Asp Val Gly Leu Lys Asp Glu Leu Val Leu Ala
85 90 95
Asp Arg Arg Leu Pro Arg Tyr Val Tyr Leu Gln Gly Glu Leu Ile Pro
100 105 110
Val Pro Met Ser Pro Pro Ala Phe Phe Gln Thr Lys Leu Leu Ser Asp
115 120 125
Trp Gly Lys Leu Arg Ala Ile Ala Gly Ala Leu Gly Phe Val Pro Pro
130 135 140
Ala Ile Gly Ala Thr Leu Ser Ala Gln Gly Asp Glu Glu Thr Val Ala
145 150 155 160
Gln Phe Phe Gly Arg His Leu Gly Gln Glu Val Leu Glu Arg Leu Val
165 170 175
Gln Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Asn Gln Leu Ser
180 185 190
Ala Ser Ala Ala Phe Gly Lys Val Thr Lys Met Ala Asp Ile Gly Gly
195 200 205
Gly Leu Ala Ala Gly Ala Ile Leu Ser Leu Ala Lys Asn Gly Arg Ala
210 215 220
Lys Lys Thr Val Asp Pro Ser Leu Pro Lys Val Gln Arg Gly Glu Leu
225 230 235 240
Ala Ser Phe Arg Gln Gly Leu Glu Ala Leu Pro Lys Ala Ile Ala Ala
245 250 255
Gln Leu Gly Asp Val Val Lys Leu Gly Trp His Leu Ile Gln Ile Lys
260 265 270
Pro Thr Glu His Gln Thr Tyr Leu Ala Glu Phe Ser Thr Pro Gln Gly
275 280 285
Pro Ala Arg Ile Glu Thr Arg Ser Met Val Leu Thr Thr Pro Ser Tyr
290 295 300
Val Ser Ala Asp Leu Leu Ala Asn Leu Cys Pro Val Ala Ser Gln Gly
305 310 315 320
Leu Ala Lys Ile Pro Tyr Pro Ala Val Ala Cys Val Val Leu Gly Tyr
325 330 335
Pro Glu Ser Ala Phe Lys Thr Ser Val Ser Ser Gly Phe Gly Asn Leu
340 345 350
Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser
355 360 365
Ser Leu Phe Pro Asn Arg Ala Pro Ala Gly Trp Arg Leu Leu Leu Asn
370 375 380
Phe Ile Gly Gly Thr Thr Asp Leu Ala Ile Ala Gln Leu Ser Gln Glu
385 390 395 400
Glu Ile Val Gln Ile Val His Arg Asp Leu Gln Lys Thr Leu Leu Lys
405 410 415
Gln Asp Ile Pro Pro Lys Val Leu Ala Val His Leu Trp Lys Arg Ala
420 425 430
Ile Pro Gln Tyr Pro Leu Gly His His Gln Asn Leu Ala Gln Ile His
435 440 445
His Ser Leu Gln Gln Arg Pro Gly Leu Phe Leu Cys Gly Asn Tyr Thr
450 455 460
Asp Gly Val Ala Val Gly Asp Cys Ile Arg Arg Gly Gln Glu Cys Ala
465 470 475 480
Ala Asp Val Val Lys Tyr Leu Asp Gln Gly
485 490
<210> 27
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Limnothrix sp. CACIAM 69d (RFP59749.1)
<400> 27
Met Asp Val Leu Ile Val Gly Ala Gly Leu Thr Gly Leu Ser Ala Ala
1 5 10 15
His Arg Leu Val Asn His Pro Gly Asn Arg Gly Arg Ser Thr Leu Val
20 25 30
Val Glu Ala Gln Asn Arg Val Gly Gly Asn Ile Thr Thr Arg Ser Ala
35 40 45
Asp Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe Ala Pro Thr Pro
50 55 60
Glu Leu Leu Lys Leu Ser Val Glu Val Gly Leu Gln Asp Glu Leu Val
65 70 75 80
Phe Ala Asp Gly Lys Leu Pro Arg Phe Val Tyr Trp Asp Gly Arg Leu
85 90 95
Gln Ala Ile Pro Met Ser Pro Gly Ala Phe Trp Asn Ser Thr Leu Leu
100 105 110
Ser Asp Arg Gly Lys Ala Arg Leu Leu Leu Gly Ala Ala Gly Phe Val
115 120 125
Pro Pro Ile Val Gly Ala Ala Ile Ser Ala Arg Gly Gly Glu Glu Thr
130 135 140
Val Arg Glu Phe Phe Thr Arg His Leu Gly Gln Glu Ala Met Glu Arg
145 150 155 160
Leu Val Asp Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp Pro Asn Ala
165 170 175
Leu Ser Ala Ser Ala Ala Phe Arg Lys Met Ala Ala Met Gln Ala Ala
180 185 190
Gly Gly Gly Leu Gly Ala Gly Ala Ala Arg Ile Leu Trp Ala Lys Arg
195 200 205
Gln Ala Ala Lys Gln Ala Pro Pro Ala Asp Pro Arg Leu Pro Lys Pro
210 215 220
Lys Ser Gly Glu Leu Gly Ser Phe Arg Glu Gly Leu Gln Ala Leu Pro
225 230 235 240
Glu Ala Val Ala Ser Gly Leu Gly Asp Arg Val Lys Leu Gly Trp Gln
245 250 255
Val Glu Ala Ile Ala Arg Asp Pro Gln Gly Thr Tyr Gln Val Thr Ile
260 265 270
Ala Ser Pro Asp Gly Pro Gln Arg Ile Thr Ala Arg Ser Ile Ile Leu
275 280 285
Ala Thr Pro Ala Pro Val Thr Ala Gln Leu Leu Glu Pro Leu Ala Pro
290 295 300
Arg Ala Ser Thr Ala Leu Asn Ala Ile Pro Tyr Pro Ala Val Ala Cys
305 310 315 320
Val Ile Leu Ala Tyr Pro Glu Thr Ala Phe Ala Gln Ser Leu Arg Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Ser Leu Gly Leu Gln Thr Leu Gly Thr
340 345 350
Ile Trp Ala Ser Ser Leu Phe Ala Gly Arg Ala Pro Gln Gly Trp Ala
355 360 365
His Leu Ile Asn Phe Ile Gly Gly Ala Gln Asn Pro Thr Leu Ile His
370 375 380
Lys Thr Glu Asp Glu Ile Ala Gln Met Val His Ala Asp Val Arg Gln
385 390 395 400
Ile Leu Leu Lys Gln Asp Val Pro Pro Lys Val Leu Ser Val Lys Leu
405 410 415
Trp Arg Arg Ala Ile Pro Gln Tyr Thr Ile Gly His Gly Ser Arg Leu
420 425 430
Ala Thr Leu His Gln Glu Leu Gln His Trp Pro Gly Leu Phe Ala Cys
435 440 445
Ser Asn Tyr Glu Gly Gly Val Ala Leu Gly Asp Cys Val Arg His Gly
450 455 460
Trp Glu Gln Ala Asp Ala Ile Glu Gln Phe Leu Gly Ser Arg Val
465 470 475
<210> 28
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Okeania hirsute (WP_124155207.1)
<400> 28
Met Thr Glu Val Leu Asp Val Leu Val Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu Thr Lys Leu Gly Asn Asn Ser Pro Leu
20 25 30
Lys Ile Leu Val Ala Glu Ser Gln Asn Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Val Ser Leu Gly Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Phe Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Gln Glu Leu Val Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp
85 90 95
Asn Gly Gln Leu Leu Pro Val Pro Met Ser Pro Thr Ala Met Leu Gln
100 105 110
Ser Lys Leu Leu Ser Asp Ala Gly Lys Leu Arg Ala Leu Val Gly Ala
115 120 125
Leu Gly Phe Val Ser Pro Ala Ile Gly Asn Leu Ser Gly Gln Gly Gly
130 135 140
Glu Glu Thr Val Ser Gln Phe Phe Gln Arg His Leu Gly Pro Glu Val
145 150 155 160
Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Ser Gln Leu Ser Ala Ser Ala Ala Phe Ala Lys Val Ala Arg Met
180 185 190
Ala Asp Leu Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala Lys
195 200 205
Lys Asn Arg Lys Phe Lys Val Ala Pro Asp Pro Asn Ile Pro Lys Thr
210 215 220
Lys Thr Gly Glu Leu Gly Ser Phe Arg Gly Gly Leu Glu Ala Leu Pro
225 230 235 240
Lys Ala Ile Ala Ser Tyr Leu Gly Glu Ala Val Lys Leu Asn Trp His
245 250 255
Leu Thr Asn Ile Arg Arg Thr Glu Gln Gln Thr Tyr Ile Ala Glu Phe
260 265 270
Ser Thr Pro Asn Gly Pro Glu Gln Ile Glu Thr Arg Thr Ile Ser Leu
275 280 285
Ser Thr Pro Ala Arg Val Cys Ala Glu Leu Phe Lys Thr Leu Gln Pro
290 295 300
Glu Ile Ala Ser Ile Phe Asn Glu Phe Tyr Tyr Pro Pro Val Ala Cys
305 310 315 320
Val Val Leu Ala Tyr Pro Asp Thr Ser Ile Lys Val Lys Ile Asp Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Ser
340 345 350
Ile Trp Ser Ser Thr Leu Phe Ser Gly Arg Thr Pro Pro Gly Trp Gln
355 360 365
Ile Phe Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro Glu Ile Ala Asn
370 375 380
Leu Asp Ser Glu Ala Ile Val Gln Gln Val His Gln Asp Leu Cys Gln
385 390 395 400
Thr Leu Leu Lys Gln Asn Ala Glu Gln Pro Lys Val Leu Ala Val His
405 410 415
Leu Trp Ser Arg Ala Ile Pro Gln Tyr Asn Leu Gly His Asn Ser Lys
420 425 430
Leu Glu Glu Ile Asn Asn Gly Leu Lys Ser Leu Pro Gly Leu Tyr Leu
435 440 445
Cys Ser Asn Tyr Ile Gly Gly Ile Ala Leu Gly Asp Cys Val Arg Arg
450 455 460
Gly Thr Glu Val Ala Thr Glu Ile Tyr Gln Gly Leu Gln Thr
465 470 475
<210> 29
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Okeania hirsute (WP_124145785.1)
<400> 29
Met Thr Glu Val Leu Asp Val Leu Val Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ala Leu Thr Lys Leu Gly Asn Asn Ser Pro Leu
20 25 30
Lys Ile Leu Val Ala Glu Ser Gln Asn Arg Val Gly Gly Asn Ile Thr
35 40 45
Thr Val Ser Leu Gly Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe
50 55 60
Ser Pro Thr Pro Glu Phe Leu Lys Leu Ala Val Asp Val Gly Leu Lys
65 70 75 80
Glu Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr Trp
85 90 95
Asn Gly Gln Leu Leu Pro Val Pro Met Ser Pro Lys Ala Met Leu Gln
100 105 110
Ser Lys Leu Leu Ser Asp Gly Gly Lys Leu Arg Ala Leu Val Gly Ala
115 120 125
Leu Gly Phe Val Ser Pro Ala Ile Gly Asp Leu Ser Glu Gln Gly Gly
130 135 140
Glu Glu Thr Val Ser Gln Phe Phe Gln Arg His Leu Gly Ala Glu Val
145 150 155 160
Met Gln Arg Leu Val Glu Pro Phe Val Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Gly Gln Leu Ser Ala Asn Ala Ala Phe Ala Lys Val Ala Arg Met
180 185 190
Ala Asp Leu Gly Gly Gly Leu Val Ala Gly Ala Leu Leu Ser Ala Lys
195 200 205
Lys Asn Arg Lys Phe Lys Val Ala Pro Asp Pro Asn Ile Pro Lys Thr
210 215 220
Lys Thr Gly Glu Leu Gly Ser Phe Arg Gly Gly Leu Glu Ala Leu Pro
225 230 235 240
Lys Ala Ile Ala Ser Tyr Leu Gly Glu Ala Val Lys Leu Asn Trp His
245 250 255
Leu Thr Gly Ile Arg Arg Thr Glu Gln Gln Thr Tyr Ile Ala Glu Phe
260 265 270
Ser Thr Pro Asn Gly Thr Glu Gln Ile Glu Thr Arg Thr Ile Ser Leu
275 280 285
Ser Thr Pro Ala Arg Val Cys Ser Glu Leu Phe Thr Thr Leu Gln Pro
290 295 300
Glu Ile Ala Ser Ile Phe Asn Glu Phe Tyr Tyr Pro Thr Val Ala Cys
305 310 315 320
Val Val Leu Ala Tyr Pro Asp Thr Ser Ile Lys Val Lys Ile Asp Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu Gly Ser
340 345 350
Ile Trp Ser Ser Thr Leu Phe Ser Gly Arg Thr Pro Pro Gly Trp Gln
355 360 365
Ile Phe Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro Glu Ile Ala Asn
370 375 380
Leu Asp Ser Glu Ala Ile Val Gln Gln Val His Gln Asp Leu Cys Gln
385 390 395 400
Thr Leu Leu Lys Gln Asn Ala Glu Gln Pro Lys Val Leu Ala Val His
405 410 415
Leu Trp Ser Arg Ala Ile Pro Gln Tyr Asn Leu Gly His Asn Ser Lys
420 425 430
Leu Glu Glu Ile Asn Asn Gly Leu Lys Ser Leu Pro Gly Leu Tyr Leu
435 440 445
Cys Ser Asn Tyr Ile Gly Gly Ile Ala Leu Gly Asp Cys Val Arg Ser
450 455 460
Gly Thr Glu Val Ala Thr Lys Ile Tyr Gln Gly Leu Gln Val
465 470 475
<210> 30
<211> 490
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Desertifilum sp. IPPAS B-1220 (WP_069967861.1)
<400> 30
Met Ser Phe Thr Asp Leu Ala Thr Pro Ser His Pro Leu Asp Thr Leu
1 5 10 15
Val Ile Gly Ala Gly Ile Ser Gly Leu Ser Leu Ala Phe Thr Leu Gln
20 25 30
Gln Arg Gln Thr Gln Val Leu Val Cys Glu Ser Gln Asn Arg Ile Gly
35 40 45
Gly Asn Ile Thr Thr Gly Gln Ala Asp Gly Phe Leu Trp Glu Glu Gly
50 55 60
Pro Asn Ser Phe Ala Pro Thr Pro Ala Leu Leu Arg Leu Ile Val Asp
65 70 75 80
Ala Gly Leu Glu Lys Asp Met Ile Leu Ala Asp Arg Arg Leu Pro Arg
85 90 95
Phe Val Tyr Arg Gln Gly Arg Leu Gln Ala Ile Pro Met Ser Pro Pro
100 105 110
Ala Ala Ile Gly Thr Pro Leu Leu Ser Trp Pro Gly Lys Leu Arg Ala
115 120 125
Gly Leu Gly Ala Ile Gly Phe Val Arg Pro Pro Leu Gly Glu Gln Phe
130 135 140
Ser Gln Gln Gly Ser Glu Glu Thr Val Ala Gln Phe Phe Gln Arg His
145 150 155 160
Leu Gly Glu Glu Val Met Glu Arg Leu Val Thr Pro Phe Val Ser Gly
165 170 175
Val Tyr Ala Gly Asp Val Asn Gln Leu Ser Ala Ala Ala Ala Phe Arg
180 185 190
Arg Ile Ala Gln Leu Glu Gly Val Gly Gly Gly Leu Val Ala Gly Ala
195 200 205
Ile Leu Ser Arg Met Lys Ala Lys Ser Gln Pro Gln Val Pro Val Asp
210 215 220
Pro Ser Leu Pro Lys Thr Arg Pro Gly Glu Leu Gly Ser Phe Gln Gln
225 230 235 240
Gly Leu Gln Met Leu Pro Gln Ala Leu Ala Ala Lys Leu Gly Asp Ala
245 250 255
Val Arg Leu Asn Trp Arg Leu Leu Gln Ile Thr Pro Thr Thr Arg Gln
260 265 270
Ser Tyr Ile Ala Glu Phe Gly Thr Pro Glu Gly Thr Gln Ile Val Glu
275 280 285
Ala Arg His Val Val Leu Thr Thr Pro Ala Tyr Ile Thr Ala Glu Ile
290 295 300
Val Glu Ala Ile Ala Pro Leu Ala Ser Gln Ala Leu Arg Ala Ile Glu
305 310 315 320
Tyr Pro Pro Val Ala Asn Val Val Leu Ala Tyr Pro Glu Ser Ala Leu
325 330 335
Lys Gln Pro Leu Lys Gly Phe Gly His Leu Ile Pro Arg Ser Glu Gly
340 345 350
Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu Phe Pro Gly Arg
355 360 365
Val Pro Pro Gly Trp Gln Leu Leu Thr Asn Tyr Ile Gly Gly Ala Thr
370 375 380
Asp Pro Gly Ile Leu Asp Leu Asp Arg Asp Ser Arg Ser Asn Leu Asp
385 390 395 400
Gly Asn Pro Ile Ala Gln Ala Val His Gln Asp Ile Arg Arg Ile Leu
405 410 415
Leu Gln Gln Glu Val Gln Pro Lys Val Leu Ala Val His Leu Trp Lys
420 425 430
Arg Ala Ile Pro Gln Tyr Asn Leu Gly His His Gln Arg Leu Gln Thr
435 440 445
Ile Phe Gln Ser Leu Ala Pro Phe Pro Gly Leu His Ile Cys Ser Asn
450 455 460
Tyr Thr Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Gln Glu
465 470 475 480
Leu Ala Asp Ile Leu Thr Gln Lys Thr Arg
485 490
<210> 31
<211> 489
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. 65AY6Li (PIK93057.1)
<400> 31
Met Glu Ala Ala Val Asn Pro Ala Thr Pro Glu Pro Leu Asn Ala Glu
1 5 10 15
Val Val Val Ile Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Trp Arg
20 25 30
Leu Gln Gln Gly Leu Ser Ala Arg Gly Gly Ser Pro Gln Ala Val Leu
35 40 45
Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln Ser
50 55 60
Lys Asp Gly Tyr Cys Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Thr
65 70 75 80
Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Thr Asp Gln Leu
85 90 95
Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Lys Glu
100 105 110
Leu Leu Pro Val Pro Leu Ser Pro Ala Ala Ala Leu Ser Ser Arg Leu
115 120 125
Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Gln Gly Leu Leu Gly Phe
130 135 140
Val Pro Pro Pro Pro Gly Arg Glu Glu Thr Val Arg Gln Phe Phe Arg
145 150 155 160
Arg Gln Leu Gly Ser Glu Val Ala Glu Arg Leu Val Glu Pro Phe Thr
165 170 175
Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Val Ala Ala
180 185 190
Phe Pro Arg Val Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe Ala
195 200 205
Gly Ala Leu Gln Ala Leu Arg Gln Arg Pro Gln Pro Ser Pro Ala Ala
210 215 220
Ile Gln Pro Pro Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly
225 230 235 240
Leu Gln Gln Leu Pro Glu Ala Leu Ala Gln Lys Leu Gly Asp Ser Leu
245 250 255
Arg Leu Gly Trp Arg Ala Val Gln Leu Lys Arg Glu Gly Glu Leu Tyr
260 265 270
Trp Val Gly Phe Glu Thr Pro Glu Gly Ser Arg Trp Val Ala Ala Arg
275 280 285
Gln Val Val Leu Ala Leu Pro Ala Tyr Glu Ala Ala Ala Leu Leu Gln
290 295 300
Glu Leu Asn Pro Pro Ala Ser Gln Leu Leu Ala Glu Ile Leu Tyr Pro
305 310 315 320
Pro Val Ala Val Val Ala Leu Ala Tyr Pro Gln Glu Ala Leu Pro Gln
325 330 335
Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg Ser Gln Gly Leu Arg
340 345 350
Thr Leu Gly Thr Ile Trp Ala Ser Cys Leu Phe Pro Glu Arg Ala Pro
355 360 365
Gln Gly Tyr His Ser Phe Leu Ser Phe Leu Gly Gly Ala Thr Asp Ala
370 375 380
Ala Leu Ala Arg Arg Arg Gly Ile Pro Pro Ile Pro Ala Leu Ser Pro
385 390 395 400
Glu Glu Arg Ala Gln Ile Ala His Ala Glu Leu Ser Gln Val Leu Leu
405 410 415
Thr Arg Arg Val Glu Pro Ile Tyr Leu Gly Glu Arg Leu Trp Pro Arg
420 425 430
Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln Arg Ile Ala Gln Val
435 440 445
Gln Ala His Leu Ala Ser Gln Thr Pro Gly Ile Trp Val Cys Ala Asn
450 455 460
Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Glu Ala
465 470 475 480
Leu Ala Gln Gln Leu Leu Ser Gln Val
485
<210> 32
<211> 489
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. 65AY6A5 (PIK88626.1)
<400> 32
Met Glu Ala Ala Val Asn Pro Ala Thr Pro Glu Pro Leu Asn Ala Glu
1 5 10 15
Val Val Val Ile Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Trp Arg
20 25 30
Leu Gln Gln Gly Leu Ser Ala Arg Gly Gly Ser Pro Gln Ala Val Leu
35 40 45
Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln Ser
50 55 60
Lys Asp Gly Tyr Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Thr
65 70 75 80
Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Thr Asp Gln Leu
85 90 95
Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Gly Ala
100 105 110
Leu Leu Pro Val Pro Leu Ser Pro Ala Ala Ala Leu Gly Ser Arg Leu
115 120 125
Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Gln Gly Leu Leu Gly Phe
130 135 140
Val Pro Pro Pro Pro Gly Arg Glu Glu Thr Val Arg Gln Phe Phe Arg
145 150 155 160
Arg Gln Leu Gly Ser Glu Val Ala Glu Arg Leu Val Glu Pro Phe Thr
165 170 175
Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Val Ala Ala
180 185 190
Phe Pro Arg Val Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe Ala
195 200 205
Gly Ala Leu Gln Ala Leu Arg Gln Arg Pro Gln Pro Ser Pro Ala Ala
210 215 220
Ile Gln Pro Pro Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly
225 230 235 240
Leu Gln Gln Leu Pro Glu Ala Leu Ala Gln Lys Leu Gly Asp Ser Leu
245 250 255
Arg Leu Gly Trp Arg Ala Leu Gln Leu Lys Arg Ala Gly Glu Leu Tyr
260 265 270
Trp Val Gly Phe Glu Thr Pro Glu Gly Ser Arg Trp Val Ala Ala Arg
275 280 285
Gln Val Val Leu Ala Leu Pro Ala Tyr Glu Ala Ala Ala Leu Leu Gln
290 295 300
Glu Leu Asn Pro Pro Ala Ser Gln Leu Leu Ala Glu Ile Leu Tyr Pro
305 310 315 320
Pro Val Ala Val Val Ala Leu Ala Tyr Pro Gln Glu Ala Leu Pro Gln
325 330 335
Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg Ser Gln Gly Leu Arg
340 345 350
Thr Leu Gly Thr Ile Trp Ala Ser Cys Leu Phe Pro Glu Arg Ala Pro
355 360 365
Gln Gly Tyr His Ser Phe Leu Ser Phe Leu Gly Gly Ala Thr Asp Ala
370 375 380
Ala Leu Ala Arg Gln Gln Gly Ile Pro Pro Ile Pro Ala Leu Ser Pro
385 390 395 400
Glu Glu Arg Ala Gln Ile Ala His Ala Glu Leu Ser Gln Val Leu Leu
405 410 415
Thr Arg Arg Ala Glu Pro Val Tyr Leu Gly Glu Arg Leu Trp Pro Arg
420 425 430
Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln Arg Ile Ala Gln Val
435 440 445
Gln Ala His Leu Ala Ser Gln Thr Pro Gly Ile Trp Val Cys Ala Asn
450 455 460
Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Glu Ala
465 470 475 480
Leu Ala Gln Gln Leu Leu Ser Gln Val
485
<210> 33
<211> 489
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. 60AY4M2 (PIK94415.1)
<400> 33
Met Glu Ala Ala Val Asn Pro Ala Thr Pro Glu Pro Leu Asn Ala Glu
1 5 10 15
Val Val Val Ile Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Trp Arg
20 25 30
Leu Gln Gln Gly Leu Ser Ala Arg Gly Gly Ser Pro Gln Ala Val Leu
35 40 45
Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln Ser
50 55 60
Lys Asp Gly Tyr Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Thr
65 70 75 80
Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Ala Asp Gln Leu
85 90 95
Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Gly Ala
100 105 110
Leu Leu Pro Val Pro Leu Ser Pro Ala Ala Ala Leu Gly Ser Arg Leu
115 120 125
Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Gln Gly Leu Leu Gly Phe
130 135 140
Val Pro Pro Pro Pro Gly Arg Glu Glu Thr Val Arg Gln Phe Phe Arg
145 150 155 160
Arg Gln Leu Gly Ser Glu Val Ala Glu Arg Leu Val Glu Pro Phe Thr
165 170 175
Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Val Ala Ala
180 185 190
Phe Pro Arg Val Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe Ala
195 200 205
Gly Ala Leu Gln Ala Leu Arg Gln Arg Pro Gln Pro Ser Pro Ala Ala
210 215 220
Ile Gln Pro Pro Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly
225 230 235 240
Leu Gln Gln Leu Pro Glu Ala Leu Ala Gln Lys Leu Gly Asp Ser Leu
245 250 255
Arg Leu Gly Trp Arg Ala Leu Gln Leu Lys Arg Ala Gly Glu Leu Tyr
260 265 270
Trp Val Gly Phe Glu Thr Pro Glu Gly Ser Arg Trp Val Ala Ala Arg
275 280 285
Gln Val Val Leu Ala Leu Pro Ala Tyr Glu Ala Ala Ala Leu Leu Gln
290 295 300
Glu Leu Asn Pro Pro Ala Ser Gln Leu Leu Ala Glu Ile Leu Tyr Pro
305 310 315 320
Pro Val Ala Val Val Ala Leu Ala Tyr Pro Gln Glu Ala Leu Pro Gln
325 330 335
Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg Ser Gln Gly Leu Arg
340 345 350
Thr Leu Gly Thr Ile Trp Ala Ser Cys Leu Phe Pro Glu Arg Ala Pro
355 360 365
Gln Gly Tyr His Ser Phe Leu Ser Phe Leu Gly Gly Ala Thr Asp Ala
370 375 380
Ala Leu Ala Arg Gln Gln Gly Ile Pro Pro Ile Pro Ala Leu Ser Pro
385 390 395 400
Glu Glu Arg Ala Gln Ile Ala His Ala Glu Leu Ser Gln Val Leu Leu
405 410 415
Thr Arg Arg Ala Glu Pro Val Tyr Leu Gly Glu Arg Leu Trp Pro Arg
420 425 430
Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln Arg Ile Ala Gln Val
435 440 445
Gln Ala His Leu Ala Ser Gln Thr Pro Gly Ile Trp Val Cys Ala Asn
450 455 460
Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Glu Ala
465 470 475 480
Leu Ala Gln Gln Leu Phe Ser Gln Val
485
<210> 34
<211> 489
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. 63AY4M1 (PIK96673.1)
<400> 34
Met Glu Ala Ala Val Asn Pro Ala Thr Pro Glu Pro Leu Asn Ala Glu
1 5 10 15
Val Val Val Ile Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Trp Arg
20 25 30
Leu Gln Gln Gly Leu Ser Ala Arg Gly Gly Ser Pro Gln Ala Val Leu
35 40 45
Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln Ser
50 55 60
Lys Asp Gly Tyr Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Thr
65 70 75 80
Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Ala Asp Gln Leu
85 90 95
Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Gly Ala
100 105 110
Leu Leu Pro Val Pro Leu Ser Pro Ala Ala Ala Leu Gly Ser Arg Leu
115 120 125
Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Gln Gly Leu Leu Gly Phe
130 135 140
Val Pro Pro Pro Pro Gly Arg Glu Glu Thr Val Arg Gln Phe Phe Arg
145 150 155 160
Arg Gln Leu Gly Ser Glu Val Ala Glu Arg Leu Val Glu Pro Phe Thr
165 170 175
Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Val Ala Ala
180 185 190
Phe Pro Arg Val Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe Ala
195 200 205
Gly Ala Leu Gln Ala Leu Arg Gln Arg Pro Gln Pro Ser Pro Ala Ala
210 215 220
Ile Gln Pro Pro Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly
225 230 235 240
Leu Gln Gln Leu Pro Glu Ala Leu Ala Gln Lys Leu Gly Asp Ser Leu
245 250 255
Arg Leu Gly Trp Arg Ala Leu Gln Leu Lys Arg Ala Gly Glu Leu Tyr
260 265 270
Trp Val Gly Phe Glu Thr Pro Glu Gly Ser Arg Trp Val Ala Ala Arg
275 280 285
Gln Val Val Leu Ala Leu Pro Ala Tyr Glu Ala Ala Ala Leu Leu Gln
290 295 300
Glu Leu Asn Pro Pro Ala Ser Gln Leu Leu Ala Glu Ile Leu Tyr Pro
305 310 315 320
Pro Val Ala Val Val Ala Leu Ala Tyr Pro Gln Glu Ala Leu Pro Gln
325 330 335
Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg Ser Gln Gly Leu Arg
340 345 350
Thr Leu Gly Thr Ile Trp Ala Ser Cys Leu Phe Pro Glu Arg Ala Pro
355 360 365
Gln Gly Tyr His Ser Phe Leu Ser Phe Leu Gly Gly Ala Thr Asp Ala
370 375 380
Ala Leu Ala Arg Arg Arg Gly Ile Pro Pro Ile Pro Ala Leu Ser Pro
385 390 395 400
Glu Glu Arg Ala Gln Ile Ala His Ala Glu Leu Ser Gln Val Leu Leu
405 410 415
Thr Arg Arg Ala Glu Pro Val Tyr Leu Gly Glu Arg Leu Trp Pro Arg
420 425 430
Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln Arg Ile Ala Gln Val
435 440 445
Gln Ala His Leu Ala Ser Gln Thr Pro Gly Ile Trp Val Cys Ala Asn
450 455 460
Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Glu Ala
465 470 475 480
Leu Ala Gln Gln Leu Phe Ser Gln Val
485
<210> 35
<211> 489
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. 63AY4M2 (PIK85371.1)
<400> 35
Met Glu Ala Ala Val Asn Pro Ala Thr Pro Glu Pro Leu Asn Ala Glu
1 5 10 15
Val Val Val Ile Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Trp Arg
20 25 30
Leu Gln Gln Gly Leu Ser Ala Arg Gly Gly Ser Pro Gln Ala Val Leu
35 40 45
Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln Ser
50 55 60
Lys Asp Gly Tyr Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Thr
65 70 75 80
Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Thr Asp Gln Leu
85 90 95
Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Gly Ala
100 105 110
Leu Leu Pro Val Pro Leu Ser Pro Ala Ala Ala Leu Gly Ser Arg Leu
115 120 125
Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Gln Gly Leu Leu Gly Phe
130 135 140
Val Pro Pro Pro Pro Gly Arg Glu Glu Thr Val Arg Gln Phe Phe Arg
145 150 155 160
Arg Gln Leu Gly Ser Glu Val Ala Glu Arg Leu Val Glu Pro Phe Thr
165 170 175
Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Val Ala Ala
180 185 190
Phe Pro Arg Val Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe Ala
195 200 205
Gly Ala Leu Gln Ala Leu Arg Gln Arg Pro Gln Pro Ser Pro Ala Ala
210 215 220
Ile Gln Pro Pro Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly
225 230 235 240
Leu Gln Gln Leu Pro Glu Ala Leu Ala Gln Lys Leu Gly Asp Ser Leu
245 250 255
Arg Leu Gly Trp Arg Ala Leu Gln Leu Lys Arg Ala Gly Glu Leu Tyr
260 265 270
Trp Val Gly Phe Glu Thr Pro Glu Gly Ser Arg Trp Val Ala Ala Arg
275 280 285
Gln Val Val Leu Ala Leu Pro Ala Tyr Glu Ala Ala Ala Leu Leu Gln
290 295 300
Glu Leu Asn Pro Pro Ala Ser Gln Leu Leu Ala Glu Ile Leu Tyr Pro
305 310 315 320
Pro Val Ala Val Val Ala Leu Ala Tyr Pro Gln Glu Ala Leu Pro Gln
325 330 335
Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg Ser Gln Gly Leu Arg
340 345 350
Thr Leu Gly Thr Ile Trp Ala Ser Cys Leu Phe Pro Glu Arg Ala Pro
355 360 365
Gln Gly Tyr His Ser Phe Leu Ser Phe Leu Gly Gly Ala Thr Asp Ala
370 375 380
Ala Leu Ala Arg Arg Arg Gly Ile Pro Pro Ile Pro Ala Leu Ser Pro
385 390 395 400
Glu Glu Arg Ala Gln Ile Ala His Ala Glu Leu Ser Gln Val Leu Leu
405 410 415
Thr Arg Arg Ala Glu Pro Val Tyr Leu Gly Glu Arg Leu Trp Pro Arg
420 425 430
Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln Arg Ile Ala Gln Val
435 440 445
Gln Ala His Leu Ala Ser Gln Thr Pro Gly Ile Trp Val Cys Ala Asn
450 455 460
Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val Arg Arg Ala Glu Ala
465 470 475 480
Leu Ala Gln Gln Leu Phe Ser Gln Val
485
<210> 36
<211> 485
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanobacteria bacterium J007 (RMH78328.1)
<400> 36
Met Ser Glu Pro Ala Asn Pro Ser Thr Leu Leu Asp Thr Leu Val Val
1 5 10 15
Gly Ala Gly Ile Thr Gly Leu Thr Leu Ala Phe Asp Leu Gln Gln Lys
20 25 30
Ser Ser Gly Thr Ala His Ser Ala Lys Val Leu Val Ala Glu Ser Gln
35 40 45
Ala Arg Val Gly Gly Arg Ile Val Thr Ala Ser Gly Asp Gly Phe Leu
50 55 60
Trp Glu Glu Gly Pro Asn Ser Phe Ala Pro Thr Pro Glu Leu Leu Gln
65 70 75 80
Leu Ala Val Glu Val Gly Leu Lys Asp Lys Leu Val Phe Ala Asp Gly
85 90 95
Lys Leu Pro Arg Phe Val Tyr Trp Gln Gly Glu Leu Met Pro Val Pro
100 105 110
Met Ser Pro Pro Ala Ile Ile Ser Ser Lys Leu Leu Thr Trp Arg Gly
115 120 125
Lys Leu Arg Ala Phe Phe Gly Ala Leu Gly Phe Val Pro Pro Ala Met
130 135 140
Ala Asp Val Gly Ser Glu Glu Thr Val Ala Ser Phe Phe Glu Arg His
145 150 155 160
Leu Gly Arg Glu Val Leu Gln Arg Leu Val Glu Pro Phe Val Ser Gly
165 170 175
Val Tyr Ala Gly Asp Pro Arg Gln Leu Ser Ala Arg Ala Ala Phe Gly
180 185 190
Arg Val Ala Arg Met Ala Glu Ala Gly Gly Gly Leu Val Ala Gly Ala
195 200 205
Val Arg Thr Ser Arg Gln Lys Pro Lys Thr Lys Val Thr Pro Asp Pro
210 215 220
Asn Val Pro Gln Pro Lys Arg Gly Gln Leu Gly Ser Phe Arg Gln Gly
225 230 235 240
Leu Ala Thr Leu Pro Glu Ala Ile Ala Asp Arg Leu Gly Asp Ala Val
245 250 255
Gln Leu Asn Trp His Leu Ile Arg Val Arg Pro Thr Glu Arg His Thr
260 265 270
Tyr Ile Ala Glu Phe Ser Thr Pro Asp Gly Pro Lys Arg Val Glu Ala
275 280 285
Arg Ser Val Val Leu Thr Thr Pro Thr Tyr Val Thr Ala Asp Leu Phe
290 295 300
Asp Pro Leu His Gly Glu Ile Ala Arg Ala Leu Arg Gly Phe Val Tyr
305 310 315 320
Pro Ser Val Ala Cys Val Val Leu Gly Tyr Pro Thr Ser Ala Leu Lys
325 330 335
Arg Pro Leu Asn Gly Phe Gly Asn Leu Ile Pro Arg Asn Gln Gly Ile
340 345 350
Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu Phe Ser Gly Arg Ala
355 360 365
Pro Glu Gly Trp Asn Leu Leu Ile Asn Phe Ile Gly Gly Thr Gly Asp
370 375 380
Pro Ala Ile Ala Glu Leu Glu Lys Asp Glu Ile Val Arg Val Val His
385 390 395 400
Arg Asp Leu Leu Lys Thr Leu Leu Ala Arg Asp Val Asp Pro Lys Val
405 410 415
Leu Ala Val His Leu Trp Lys Arg Ala Ile Pro Gln Tyr Cys Leu Gly
420 425 430
His His Gln Arg Trp Glu Gln Ile Asp Arg Gly Leu Gln Glu Phe Pro
435 440 445
Gly Leu Tyr Leu Cys Gly Asn Tyr Ser Asp Gly Val Ala Val Gly Asp
450 455 460
Cys Val Arg Arg Ala Arg Asp Arg Ala Ala Glu Ile Arg Gln Tyr Leu
465 470 475 480
Ala Ser Ala Thr Val
485
<210> 37
<211> 468
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Spirulina major (WP_072619201.1)
<400> 37
Met Leu Asp Ser Leu Ile Val Gly Ala Gly Leu Ser Gly Leu Thr Val
1 5 10 15
Ala His Thr Leu Gln Gln Arg Asp Arg Asn Ile Leu Val Ala Glu Ala
20 25 30
Ser Pro Asp Val Gly Gly Asn Ile Ile Ser Arg Gln Gln Gly Glu Phe
35 40 45
Leu Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro Asn Pro Thr Leu Leu
50 55 60
Lys Leu Ala Val Asp Val Gly Leu Lys Asp Asp Leu Val Phe Ala Asp
65 70 75 80
Arg Lys Leu Pro Arg Trp Val Tyr Trp Gln Gly Arg Leu Leu Ala Val
85 90 95
Pro Met Ser Pro Gly Thr Ala Val Arg Ser Pro Leu Leu Ser Val Pro
100 105 110
Gly Lys Leu Arg Ala Leu Phe Gly Ala Leu Gly Phe Val Pro Pro Leu
115 120 125
Val Gly Ser His Ile Gln Ala Gln Gly Gly Asp Glu Thr Ile Trp Gln
130 135 140
Phe Ile Asn Arg His Leu Gly Pro Glu Val Ala Glu Arg Leu Ile Ser
145 150 155 160
Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Val His Ala Leu Ser Met
165 170 175
Ala Ala Ala Phe Arg Lys Ile Tyr Arg Leu Glu Thr Leu Gly Gly Gly
180 185 190
Leu Val Ala Gly Ala Met Arg Ser Arg Arg Gln Gly Lys Ala Asp Arg
195 200 205
Pro Ala Val Asp Pro Asn Leu Pro Thr Thr Lys Pro Gly Gln Leu Gly
210 215 220
Ser Phe Arg Glu Gly Met Val Met Leu Pro Asn Ala Ile Ala Ala Arg
225 230 235 240
Leu Gly Asp Arg Leu Arg Cys Arg Trp Thr Leu Thr Gln Ile Glu Pro
245 250 255
Leu Glu Gln Gly Tyr Arg Ala His Phe Asp Thr Pro Asp Gly Gly Gln
260 265 270
Thr Ile Glu Thr Arg Thr Leu Val Leu Ala Ile Pro Ala His Arg Val
275 280 285
Ala Pro Leu Leu Ala Pro Leu Met Pro Glu Leu Ser Thr Thr Leu Gln
290 295 300
Ala Ile Pro Tyr Pro Ala Val Ala Cys Thr Val Met Ala Tyr Pro Lys
305 310 315 320
Thr Ala Leu Val Arg Ser Leu His Gly Phe Gly Asn Leu Asn Pro Arg
325 330 335
Ser Gln Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Thr Leu Phe
340 345 350
Pro Gly Arg Ala Pro Glu Gly Trp Val Met Leu Ser Ser Phe Ile Gly
355 360 365
Gly Ser Thr Asp Pro Ala Val Ala Thr Met Asp Glu Gly Ala Ile Ala
370 375 380
Gln Ala Val His Gln Asp Leu Ser Asn Ile Leu Val Lys Pro Asp Ser
385 390 395 400
Thr Pro Lys Val Leu Ala Val Lys Leu Trp Ser Lys Ala Ile Pro Gln
405 410 415
Tyr Thr Leu Gly His Cys Asp Arg Leu Ser Val Met Ala Glu His Leu
420 425 430
Lys Ala His Pro His Leu Thr Leu Cys Ser Asn Tyr Thr Asp Gly Val
435 440 445
Ala Leu Gly Asp Cys Ile Arg Arg Gly Ile Glu Ala Gly Glu Ala Ile
450 455 460
Asp Gln Gln Leu
465
<210> 38
<211> 463
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Euhalothece sp. KZN 001 (PNW65677.1)
<400> 38
Met Val Glu Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu Thr Thr
1 5 10 15
Ala Tyr Arg Leu His Glu Gln Gln His Glu Val Met Val Ala Glu Lys
20 25 30
Asn Asp Arg Ala Gly Gly Asn Ile Thr Ser Lys Ala Ser Gly Gly Phe
35 40 45
Leu Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro Thr Pro Glu Leu Leu
50 55 60
Lys Leu Ala Val Asp Val Gly Leu Lys Asp Glu Phe Val Phe Ala Asp
65 70 75 80
Pro Thr Leu Pro Arg Tyr Val Tyr Trp Gln Gly Lys Leu Leu Pro Val
85 90 95
Pro Met Ser Pro Pro Ser Ala Val Lys Ser Gln Leu Leu Ser Pro Leu
100 105 110
Gly Lys Leu Arg Ala Leu Thr Gly Ala Ile Gly Phe Val Pro Pro Lys
115 120 125
Val Ala Thr Glu Glu Glu Thr Val Ala Glu Phe Phe Thr Arg His Leu
130 135 140
Gly Ser Glu Val Ala Gln Arg Leu Val Ser Pro Phe Val Ser Gly Val
145 150 155 160
Tyr Ala Gly Asp Val Ala Asn Leu Ser Ala Ser Ala Ala Phe Gly Arg
165 170 175
Val Thr Gln Leu Ala Asp Val Gly Gly Gly Leu Val Ala Gly Ala Ile
180 185 190
Leu Ser Arg Gly Lys Lys Lys Lys Ala Thr Thr Glu Val Asp Pro Glu
195 200 205
Ile Pro Lys Thr Arg Ser Gly Glu Leu Gly Ser Phe Arg Glu Gly Leu
210 215 220
Gln Gln Leu Pro Ser Ser Ile Ala Arg Lys Leu Gly Glu Ala Val Lys
225 230 235 240
Phe Asn Trp Glu Leu Lys Gln Ile Ser Gln Thr Ser Glu Ala Gly Tyr
245 250 255
Ile Ala Thr Phe Ser Thr Pro Glu Gly Glu Gln Asn Val Glu Ala Gln
260 265 270
Gln Ile Ile Leu Thr Thr Pro Ala Tyr Val Ser Ala Pro Ile Leu Gln
275 280 285
Asp Leu Ser Pro Glu Ala Ser Gln Ala Leu Arg Glu Ile Tyr Tyr Pro
290 295 300
Pro Val Ala Cys Val Val Leu Ala Tyr Pro Asp Glu Ala Phe Ser Val
305 310 315 320
Pro Leu Asp Gly Phe Gly Asn Leu Asn Pro Arg Ser Glu Gly Val Arg
325 330 335
Thr Leu Gly Thr Ile Trp Ser Ser Thr Leu Phe Ser Gly Arg Thr Pro
340 345 350
Gln Gly Trp Gln Ile Leu Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro
355 360 365
Glu Ile Ala Gln Leu Ser Glu Glu Glu Ile Val Gln Gln Val His Gln
370 375 380
Asp Leu Gln Lys Thr Ile Val Lys Pro Asn Thr Thr Pro Lys Pro Leu
385 390 395 400
Ala Val His Leu Trp Ser Gln Ala Ile Pro Gln Tyr Thr Leu Gly His
405 410 415
Leu Asp Arg Ile Ala Arg Ile Lys Glu Ser Leu Lys Pro Phe Ser Gly
420 425 430
Leu Phe Leu Ser Ser Asn Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys
435 440 445
Val Arg Arg Gly Glu Glu Thr Ala Ala Gln Ile Leu Asn Lys Lys
450 455 460
<210> 39
<211> 461
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Dactylococcopsis salina (WP_015230904.1)
<400> 39
Met Val Glu Thr Leu Ile Val Gly Ala Gly Ile Ser Gly Leu Thr Thr
1 5 10 15
Ala Tyr Arg Leu His Glu Gln Gln His Glu Val Met Val Ala Glu Lys
20 25 30
Asn Asp Arg Ala Gly Gly Asn Ile Thr Ser Lys Ala Ser Gly Gly Phe
35 40 45
Leu Trp Glu Glu Gly Pro Asn Ser Phe Ser Pro Thr Pro Glu Leu Leu
50 55 60
Lys Leu Ala Val Asp Val Gly Leu Lys Asp Glu Phe Val Phe Ala Asp
65 70 75 80
Pro Thr Leu Pro Arg Tyr Val Tyr Trp Gln Gly Lys Leu Leu Pro Val
85 90 95
Pro Met Ser Pro Pro Ala Ala Val Lys Ser Gln Leu Leu Ser Pro Leu
100 105 110
Gly Lys Leu Arg Ala Leu Thr Gly Ala Ile Gly Phe Val Pro Pro Lys
115 120 125
Val Ala Thr Glu Glu Glu Thr Val Ala Glu Phe Phe Thr Arg His Leu
130 135 140
Gly Ser Glu Val Ala Gln Arg Leu Val Ser Pro Phe Val Ser Gly Val
145 150 155 160
Tyr Ala Gly Asp Val Ala Asn Leu Ser Ala Ser Ala Ala Phe Ala Arg
165 170 175
Val Thr Gln Leu Ala Asp Val Gly Gly Gly Leu Val Ala Gly Ala Ile
180 185 190
Leu Ser Arg Gly Lys Lys Lys Lys Ala Thr Thr Glu Val Asn Ser Glu
195 200 205
Ile Pro Lys Thr Arg Ser Gly Glu Leu Gly Ser Phe Arg Glu Gly Leu
210 215 220
Gln Gln Leu Pro Ser Ser Ile Ala Arg Lys Leu Gly Glu Ala Val Lys
225 230 235 240
Phe Asn Trp Glu Leu Lys Gln Ile Ser Gln Thr Ser Glu Ala Gly Tyr
245 250 255
Ile Ala Thr Phe Ser Thr Pro Glu Gly Glu Gln Asn Val Glu Ala Gln
260 265 270
Gln Ile Ile Leu Thr Thr Pro Ala Tyr Val Asn Ala Pro Ile Leu Gln
275 280 285
Asp Leu Ser Pro Glu Ala Ser Gln Ala Leu Arg Glu Ile Asp Tyr Pro
290 295 300
Pro Val Ala Cys Val Val Leu Ala Tyr Pro Asp Glu Ala Phe Ser Val
305 310 315 320
Pro Leu Asp Gly Phe Gly Asn Leu Asn Pro Arg Ser Glu Gly Val Arg
325 330 335
Thr Leu Gly Thr Ile Trp Ser Ser Thr Leu Phe Ser Gly Arg Thr Pro
340 345 350
Gln Gly Trp Gln Ile Leu Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro
355 360 365
Glu Ile Ala Gln Leu Ser Glu Glu Glu Ile Val Gln Gln Val His Gln
370 375 380
Asp Leu Gln Lys Thr Ile Val Lys Pro Asn Thr Thr Pro Lys Pro Leu
385 390 395 400
Ala Val His Leu Trp Ser Gln Ala Ile Pro Gln Tyr Thr Leu Gly His
405 410 415
Leu Asp Arg Ile Ala Arg Ile Lys Glu Ser Leu Lys Pro Phe Ser Gly
420 425 430
Leu Phe Leu Ser Ser Asn Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys
435 440 445
Val Arg Arg Gly Glu Glu Thr Ala Ala Gln Ile Leu Arg
450 455 460
<210> 40
<211> 467
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. PCC 7336 (WP_017328280.1)
<400> 40
Met Gly Ser His Thr Pro Asp Ile Leu Ile Leu Gly Ala Gly Ile Ser
1 5 10 15
Gly Leu Ser Ala Ala Phe Arg Leu His Gln Gln Gln Gln Asp Leu Leu
20 25 30
Val Ala Glu Arg Ala Glu Arg Val Gly Gly Val Ile Thr Thr Arg Ala
35 40 45
Gln Asp Gly Phe Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro Ser
50 55 60
Pro Ala Leu Leu Asn Leu Ile Ala Asp Ala Gly Ile Ala Asp Arg Leu
65 70 75 80
Leu Trp Ala Asp Gly Lys Leu Pro Arg Phe Val Tyr Leu Glu Gly Lys
85 90 95
Leu Thr Leu Val Pro Met Thr Pro Pro Asp Leu Ile Lys Ser Asn Leu
100 105 110
Leu Ser Phe Gly Ala Lys Leu Arg Ala Leu Leu Gly Ile Leu Gly Phe
115 120 125
Thr Ala Lys Ala Pro Asp Lys Glu Glu Thr Val Glu Glu Phe Phe Ala
130 135 140
Arg Gln Leu Gly Pro Gln Val Val Glu Arg Leu Val Gly Pro Phe Thr
145 150 155 160
Ser Gly Val Tyr Ala Gly Asp Thr Gln Gln Leu Ser Ala Thr Ala Ala
165 170 175
Phe Ser Lys Val Ala Asp Leu Glu Arg Lys Tyr Gly Ser Ile Ile Ala
180 185 190
Gly Ile Ile Arg Ser Pro Lys Ser Pro Lys Pro Pro Ile Ser Ala Lys
195 200 205
Ile Asp Pro Leu Pro Lys Arg Gly Gln Leu Gly Asn Phe Val Glu Gly
210 215 220
Leu Gln Glu Leu Pro Asp Ala Ile Ala Gln Gln Leu Gly Asp Ala Val
225 230 235 240
Lys Leu Gln Trp Glu Ala Ala Glu Ile Val Lys Glu Gly Asp Arg Tyr
245 250 255
Arg Thr Thr Phe Gln Thr Pro Ser Gly Pro Gln Thr Val Ser Ser Lys
260 265 270
Ala Ile Leu Leu Ala Val Pro Ala Tyr Arg Ala Ala Pro Leu Leu Lys
275 280 285
Ser Leu Asp Thr Ala Leu Ala Asp Glu Leu Ala Ala Ile Pro Tyr Pro
290 295 300
His Val Gly Ala Val Thr Leu Ala Tyr Pro Ala Asp Ala Leu Pro Gln
305 310 315 320
Pro Phe Ala Gly Phe Gly Gln Leu Phe Pro Arg Gly Gln Gly Ile Arg
325 330 335
Thr Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro
340 345 350
Ala Gly Tyr Gln Cys Thr Leu Ser Tyr Ile Gly Gly Ala Thr Asp Pro
355 360 365
Asp Ile Ala Gln Met Thr Asp Glu Ala Leu Ala Arg Thr Val His Gln
370 375 380
Asp Leu Ser Lys Thr Leu Leu Val Lys Glu Ala Glu Pro Arg Val Met
385 390 395 400
Gly Val Arg Arg Trp Pro Arg Ala Ile Pro Gln Tyr Thr Leu Gly His
405 410 415
Arg Gln Arg Leu Ala Arg Ile Asp Glu Leu Leu Ala Asp Tyr Ser Gly
420 425 430
Leu Val Leu Cys Thr Asn Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys
435 440 445
Val Arg Arg Gly Glu Ala Arg Ala Ala Asp Leu Val Glu Trp Leu Ala
450 455 460
Gln Ala Glu
465
<210> 41
<211> 484
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Arthrospira sp. O9.13F (WP_111891435)
<400> 41
Met Thr Asn Leu Val Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala Tyr Ser Leu Asn Arg Glu Lys Ser Val Arg Glu Pro
20 25 30
Leu Lys Val Leu Val Thr Glu Ser Gln Asn Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Gly Arg Ala Asp Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ala Pro Thr Pro Glu Leu Leu Gly Leu Ala Val Asp Leu Gly Leu
65 70 75 80
Lys Glu Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Asn Leu Met Leu His Pro Val Pro Met Asn Pro Pro Ala Leu Leu
100 105 110
Ser Ser Glu Leu Ile Ser Ala Arg Gly Lys Leu Arg Ala Ala Leu Gly
115 120 125
Ala Ile Gly Phe Val Pro Pro Pro Val Gly Ala His Leu Ser Gln Gln
130 135 140
Gly Gly Glu Glu Thr Ile Thr Gln Phe Phe Asp Arg His Leu Gly Ser
145 150 155 160
Glu Val Leu Glu Arg Leu Val Gln Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ala Val Arg Ser Ala Phe Ser Arg Ile Val
180 185 190
Ala Ala Glu Glu Ala Gly Gly Gly Leu Leu Pro Gly Phe Val Arg Ser
195 200 205
Arg Leu Asn Lys Lys Ala Pro Val Ser Thr Pro Asp Pro Asn Ile Pro
210 215 220
Lys Thr Arg Pro Gly Glu Leu Gly Ser Phe Arg Tyr Gly Leu Gln Thr
225 230 235 240
Leu Pro Glu Thr Leu Ala Ser Lys Leu Gly Asp Arg Val Lys Leu Asn
245 250 255
Trp Thr Ile Asp Arg Phe Tyr Pro Thr Asp His Gln Thr Tyr Ile Ala
260 265 270
Glu Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Ala Arg Thr Leu
275 280 285
Ala Leu Met Thr Pro Ala His Val Ser Ala Arg Leu Leu Gln Pro Leu
290 295 300
His Ser Pro Ile Ala Thr Ala Leu Ser Gln Ile Pro Tyr Pro Pro Val
305 310 315 320
Ala Cys Val Val Leu Ala Tyr Pro Lys Ser Ala Leu Lys Gln Gln Leu
325 330 335
Lys Gly Phe Gly Asn Leu Ile Pro Arg Arg Gln Gly Ile Arg Thr Leu
340 345 350
Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu Ser
355 360 365
Trp Gln Val Leu Ser Asn Tyr Ile Gly Gly Ala Thr Asp Pro Glu Ile
370 375 380
Gly Glu Met Asp Asp Asp Gln Ile Val Ala Ala Val His Gln Asp Leu
385 390 395 400
Arg Gln Ile Leu Leu Ala Glu Asp Val Pro Pro Lys Val Leu Ala Val
405 410 415
His Leu Trp Arg Arg Ala Ile Pro Gln Tyr Thr Leu Gly His Gln Asn
420 425 430
Arg Leu Asn Cys Ile Asp Ala Gly Leu Arg Ser Xaa Pro Gly Leu Tyr
435 440 445
Leu Cys Ser Asn Tyr Ile Asp Gly Val Ser Val Gly Asp Cys Val Arg
450 455 460
Arg Gly Gln Gln Trp Ala Ser Lys Ile Gln Ser His Leu His Asp Cys
465 470 475 480
Gln Thr Ala Asn
<210> 42
<211> 484
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Arthrospira platensis (WP_006622155)
<400> 42
Met Thr Asn Leu Val Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala Tyr Ser Leu Asn Arg Glu Lys Ser Val Arg Glu Pro
20 25 30
Leu Lys Val Leu Val Thr Glu Ser Gln Asn Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Gly Arg Ala Asp Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ala Pro Thr Pro Glu Leu Leu Gly Leu Ala Val Asp Leu Gly Leu
65 70 75 80
Lys Glu Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Asn Leu Met Leu His Pro Val Pro Met Asn Pro Pro Ala Leu Leu
100 105 110
Ser Ser Glu Leu Ile Ser Ala Arg Gly Lys Leu Arg Ala Ala Leu Gly
115 120 125
Ala Ile Gly Phe Val Pro Pro Pro Val Gly Ala His Leu Ser Gln Gln
130 135 140
Gly Gly Glu Glu Thr Ile Thr Gln Phe Phe Asp Arg His Leu Gly Ser
145 150 155 160
Glu Val Leu Glu Arg Leu Val Gln Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ala Val Arg Ser Ala Phe Ser Arg Ile Val
180 185 190
Ala Ala Glu Glu Ala Gly Gly Gly Leu Leu Pro Gly Phe Val Arg Ser
195 200 205
Arg Leu Asn Lys Lys Ala Pro Val Ser Thr Pro Asp Pro Asn Ile Pro
210 215 220
Lys Thr Arg Pro Gly Glu Leu Gly Ser Phe Arg Tyr Gly Leu Gln Thr
225 230 235 240
Leu Pro Glu Thr Leu Ala Ser Lys Leu Gly Asp Arg Val Lys Leu Asn
245 250 255
Trp Thr Ile Asp Arg Phe Tyr Pro Thr Asp His Gln Thr Tyr Ile Ala
260 265 270
Glu Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Ala Arg Thr Leu
275 280 285
Ala Leu Met Thr Pro Ala His Val Ser Ala Arg Leu Leu Gln Pro Leu
290 295 300
His Ser Pro Ile Ala Thr Ala Leu Ser Gln Ile Pro Tyr Pro Pro Val
305 310 315 320
Ala Cys Val Val Leu Ala Tyr Pro Lys Ser Ala Leu Lys Gln Gln Leu
325 330 335
Lys Gly Phe Gly Asn Leu Ile Pro Arg His Gln Gly Ile Arg Thr Leu
340 345 350
Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu Ser
355 360 365
Trp Gln Val Leu Ser Asn Tyr Ile Gly Gly Ala Thr Asp Pro Glu Ile
370 375 380
Gly Glu Met Asp Asp Asp Gln Ile Val Ala Ala Val His Gln Asp Leu
385 390 395 400
Arg Gln Ile Leu Leu Ala Glu Asp Val Pro Pro Lys Val Leu Ala Val
405 410 415
His Leu Trp Arg Arg Ala Ile Pro Gln Tyr Thr Leu Gly His Gln Asn
420 425 430
Arg Leu Asn Cys Ile Asp Ala Gly Leu Arg Ser Leu Pro Gly Leu Tyr
435 440 445
Leu Cys Ser Asn Tyr Ile Asp Gly Val Ser Val Gly Asp Cys Val Arg
450 455 460
Arg Gly Gln Gln Trp Ala Ser Lys Ile Gln Ser His Leu His Asp Cys
465 470 475 480
Gln Thr Ala Asn
<210> 43
<211> 483
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Arthrospira platensis (WP_006617829)
<400> 43
Met Thr Asn Leu Val Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ser Leu Asn Arg Glu Lys Asn Pro Arg Ser Pro
20 25 30
Leu Lys Val Leu Val Thr Glu Ser Gln Asn Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Gly Arg Ala Ser Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ala Pro Thr Pro Glu Leu Leu Gly Leu Ala Val Asp Leu Gly Leu
65 70 75 80
Lys Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Asn His Lys Leu His Pro Val Pro Met Thr Pro Pro Ala Leu Leu
100 105 110
Ser Ser Gln Leu Ile Ser Pro Arg Gly Lys Leu Arg Ala Ala Leu Gly
115 120 125
Ala Ile Gly Phe Val Pro Pro Pro Val Gly Ala His Leu Ser Gln Gln
130 135 140
Arg Gly Glu Glu Thr Ile Thr Gln Phe Phe His Arg His Leu Gly Ser
145 150 155 160
Glu Val Leu Glu Arg Leu Val Gln Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ala Val Arg Ser Ala Phe Ser Arg Leu Val
180 185 190
Ala Ala Glu Asp Ala Gly Gly Ala Leu Leu Pro Gly Phe Val Arg Ser
195 200 205
Arg Leu Asn Lys Lys Ala Thr Lys Asp Thr Thr Ala Asp Pro Asn Ile
210 215 220
Pro Lys Thr Arg Pro Gly Glu Leu Gly Ser Phe Arg Tyr Gly Leu Glu
225 230 235 240
Thr Leu Pro Glu Thr Leu Ala Ser Lys Leu Gly Asp Arg Val Lys Leu
245 250 255
Asn Trp Thr Leu Asp Arg Phe Tyr Pro Thr Asp His Gln Thr Tyr Ile
260 265 270
Ala Glu Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Thr Arg Thr
275 280 285
Leu Ala Leu Met Thr Pro Ala His Val Ser Ala Arg Leu Leu Gln Pro
290 295 300
Leu His Ser Gln Ile Ala Ser Ala Leu Ser Gln Ile Pro Tyr Pro Pro
305 310 315 320
Val Ala Cys Val Val Leu Ala Tyr Pro Lys Ser Ala Leu Lys Gln Gln
325 330 335
Leu Lys Gly Phe Gly Asn Leu Ile Pro Arg His Gln Gly Ile Arg Thr
340 345 350
Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu
355 360 365
Ser Trp Gln Val Leu Ser Asn Tyr Ile Gly Gly Ala Thr Asp Pro Glu
370 375 380
Ile Gly Glu Met Asp Asp Asp Gln Ile Val Ala Ala Val His Gln Asp
385 390 395 400
Leu Arg Gln Ile Leu Leu Ala Glu Asp Val Pro Pro Lys Val Leu Ala
405 410 415
Val His Leu Trp Arg Arg Ala Ile Pro Gln Tyr Thr Leu Gly His Gln
420 425 430
Asp Arg Leu Asn Ser Ile Asn Ala Gly Leu Arg Ser Leu Pro Gly Leu
435 440 445
Tyr Leu Cys Ser Asn Tyr Ile Asp Gly Val Ser Val Gly Asp Cys Val
450 455 460
Arg Arg Gly Gln Gln Trp Ala Ser Gln Ile Gln Ser His Leu His Pro
465 470 475 480
Thr Ala Asn
<210> 44
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. BC1403 (WP_103669271.1)
<400> 44
Met Thr Ile Thr Pro Pro Ser Glu Asn Asn Gln Pro Ile Asp Val Leu
1 5 10 15
Val Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Ala
20 25 30
Ile Ser Lys Lys Tyr Ser Val Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Ser Ala Lys Asn Asp Glu Gly Tyr Gln Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Gln Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Thr
100 105 110
Pro Ala Ser Ala Ile Ala Ser Lys Ile Leu Thr Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Ile Gly Phe Ala Arg Pro Ala Met Ala Gly
130 135 140
Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Leu Leu Gly Arg Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Lys Ala Ala Phe Ser Lys Ile Ala Arg Leu
180 185 190
Glu Thr Tyr Gly Gly Leu Ile Ala Gly Ala Ile Leu Ser Ser Lys Gln
195 200 205
Arg Lys Ala Glu Lys Ile Asn Asp Pro Asn Ile Pro Lys Thr Lys Ala
210 215 220
Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Lys Met Leu Pro Glu Ala
225 230 235 240
Ile Ala Thr Lys Leu Arg Glu Gln Gly Ser Ala Ile Lys Gln Gln Trp
245 250 255
Thr Leu Arg Ser Leu Glu Lys Gln Gly Glu Val Tyr Ile Ser Lys Phe
260 265 270
Asp Thr Pro Thr Gly Glu Glu Thr Val Thr Ser Arg Ser Ile Val Leu
275 280 285
Ala Thr Pro Ala Tyr Val Thr Ala Lys Leu Leu Gln Asp Tyr Leu Pro
290 295 300
Ala Ala Ser Gln Ala Leu Asn Glu Ile Phe Tyr Pro Thr Val Ala Cys
305 310 315 320
Val Val Leu Ala Tyr Pro Lys Ser Glu Phe Ala Tyr Asp Met Lys Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly Thr
340 345 350
Ile Trp Ser Ser Ser Leu Phe Thr Gly Arg Ala Pro Glu Gly Trp Gln
355 360 365
Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala Lys
370 375 380
Leu Ser Glu Pro Glu Ile Ile Ala Ala Val His Gln Asp Leu Lys Lys
385 390 395 400
Thr Ile Leu Arg Pro Asp Thr Lys Ala Glu Pro Lys Ala Ile Ala Val
405 410 415
His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu Glu
420 425 430
Arg Leu Ala Ile Val Glu Ala Glu Leu Gln Lys Ser Gln Gly Leu Tyr
435 440 445
Val Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile Lys
450 455 460
Arg Ser Leu Gln Glu Ala Asn Lys Ile Asp Ala Tyr Leu Lys
465 470 475
<210> 45
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. 'Roaring Creek' (WP_055076288)
<400> 45
Met Thr Ser Ala Gln Ile Pro Glu Val Asn Pro Leu Asp Val Leu Val
1 5 10 15
Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Ala Ile
20 25 30
Ala Lys Asn Tyr Arg Val Leu Val Ala Glu Thr Gln Asp Arg Val Gly
35 40 45
Gly Ala Ile Thr Ser Ala Lys Asn Asp Asp Gly Tyr Gln Trp Glu Glu
50 55 60
Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala Val
65 70 75 80
Glu Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu Pro
85 90 95
Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser Pro
100 105 110
Pro Thr Ala Ile Ala Ser Arg Ile Leu Thr Trp Gly Gly Lys Ile Arg
115 120 125
Leu Ala Leu Gly Ala Met Gly Phe Ala Arg Pro Ala Met Ala Gly Glu
130 135 140
Glu Ser Val Asp Arg Phe Phe Ser Arg Leu Leu Gly Arg Gln Ala Val
145 150 155 160
Asp Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp Pro
165 170 175
Lys Arg Leu Ser Ala Lys Ala Ala Phe Ala Lys Ile Ala Lys Leu Glu
180 185 190
Thr Tyr Gly Gly Leu Leu Ala Gly Ala Ile Leu Ser Ser Lys Glu Arg
195 200 205
Lys Gln Lys Leu Asn Asp Pro Arg Ile Pro Lys Thr Lys Ala Gly Glu
210 215 220
Leu Gly Ser Phe Arg Glu Gly Ile Lys Met Leu Pro Glu Ala Ile Ala
225 230 235 240
Ala Lys Leu Arg Ala Gln Gly Thr Ala Val Lys Gln Gln Trp Thr Leu
245 250 255
Arg Ser Leu Glu Lys Gln Gly Gly Ile Tyr Ile Ser Lys Phe Asp Thr
260 265 270
Pro Thr Gly Glu Glu Ile Val Thr Ser Arg Ser Val Val Leu Ser Thr
275 280 285
Pro Ala Tyr Val Thr Ala Lys Leu Leu Gln Asp Tyr Leu Pro Ala Ala
290 295 300
Ser Gln Ala Leu Asn Glu Ile Phe Tyr Pro Thr Val Ala Cys Val Val
305 310 315 320
Leu Ala Tyr Pro Lys Ser Glu Phe Leu Tyr Asp Met Lys Gly Phe Gly
325 330 335
Asn Leu Ile Pro Arg Thr Glu Gly Val Arg Thr Leu Gly Thr Ile Trp
340 345 350
Ser Ser Ser Leu Phe Ser Gly Arg Ala Pro Ala Gly Trp Gln Leu Leu
355 360 365
Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala His Leu Ser
370 375 380
Glu Ala Glu Ile Ile Ala Ala Val His Gln Asp Leu Lys Lys Thr Ile
385 390 395 400
Leu Arg Pro Asp Thr Lys Val Ser Pro Lys Ala Ile Ala Val His Val
405 410 415
Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu Glu Arg Leu
420 425 430
Ala Thr Val Glu Ala Glu Leu Gln Lys Ser Ser Gly Leu Tyr Val Ser
435 440 445
Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile Lys Arg Ser
450 455 460
Leu Gln Glu Ala His Lys Ile Ala Ala Phe Leu Glu Thr Ile
465 470 475
<210> 46
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp.( HBC40803.1)
<400> 46
Met Thr Ser Ala Gln Thr Thr Glu Val Asn Gln Pro Leu Asp Val Leu
1 5 10 15
Val Val Gly Ala Gly Ile Ser Gly Leu Ala Ile Ala His Glu Leu Ala
20 25 30
Ile Ala Lys Asn Tyr Arg Val Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Ser Asn Arg Asn Asp Asp Gly Tyr Leu Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Glu Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser
100 105 110
Pro Pro Thr Ala Ile Ala Ser Lys Ile Leu Thr Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Leu Gly Phe Ala Arg Pro Ala Met Ser Gly
130 135 140
Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Leu Leu Gly Lys Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Arg Ala Ala Phe Ser Lys Ile Phe Arg Leu
180 185 190
Glu Asn Gly Tyr Gly Gly Leu Leu Thr Gly Ala Ile Leu Thr Ala Lys
195 200 205
Asp Arg Lys Ala Gln Lys Leu Asn Asp Pro Asn Ile Pro Lys Val Lys
210 215 220
Ser Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Lys Met Leu Pro Glu
225 230 235 240
Ala Ile Ala Thr Lys Leu Arg Asp Gln Gly Thr Ala Val Lys Gln Gln
245 250 255
Trp Thr Leu Arg Ser Leu Glu Lys Gln Gly Glu Ile Tyr Val Ser Gln
260 265 270
Phe Gly Thr Pro Thr Gly Thr Glu Thr Ile Thr Ser Arg Ser Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Ser Ala Lys Leu Leu Gln Gly Tyr Leu
290 295 300
Pro Ala Ala Ser Gln Ala Leu Ser Glu Ile Phe Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Lys Ser Glu Phe Ala Tyr Asp Met Lys
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly
340 345 350
Thr Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Asp Gly Trp
355 360 365
Gln Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Asp Leu Ala
370 375 380
Asn Leu Ser Glu Ala Lys Ile Val Gln Ala Val His Gln Asp Leu Lys
385 390 395 400
Lys Thr Leu Leu Arg Pro Asp Ser Lys Val Glu Pro Lys Val Ile Ala
405 410 415
Val His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu
420 425 430
Glu Arg Leu Ala Thr Ile Glu Thr Glu Leu Gln Lys Ser Gln Gly Leu
435 440 445
Tyr Val Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile
450 455 460
Lys Arg Ser Leu Gln Glu Ala Asp Lys Ile Ala Ala Phe Leu Lys
465 470 475
<210> 47
<211> 511
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Synechococcus sp. JA-2-3B'a(2-13) (WP_049749573)
<400> 47
Met Asn Ser Ser Val Glu His Phe Ser Gly Thr Gln Ser Leu Gln Ala
1 5 10 15
Glu Val Val Val Val Gly Ala Gly Ile Ser Gly Leu Thr Leu Ala Leu
20 25 30
Arg Leu Gln Gln Gly Leu Ser Pro Lys Asp Glu Ser Thr Gln Pro Leu
35 40 45
Leu Leu Ala Glu Ala Ser Ser Arg Val Gly Gly Cys Ile Ser Thr Gln
50 55 60
Ser Lys Asp Ser Tyr Arg Trp Glu Glu Gly Pro Asn Ser Phe Thr Pro
65 70 75 80
Val Pro Ala Leu Leu Asn Leu Ile Ala Glu Val Gly Leu Ala Glu His
85 90 95
Leu Val Leu Ala Asp Ala Lys Leu Pro Arg Tyr Ile Tyr Trp Glu Lys
100 105 110
Glu Leu Leu Pro Val Pro Leu Ser Pro Ser Ala Ala Ile Gly Ser Arg
115 120 125
Leu Leu Ser Val Gly Gly Lys Leu Arg Ala Leu Arg Gly Leu Leu Gly
130 135 140
Phe Val Ala Pro Pro Pro Gly Gly Glu Glu Thr Val Arg Gln Phe Phe
145 150 155 160
Arg Arg Gln Leu Gly Ser Glu Val Val Glu Arg Leu Val Glu Pro Phe
165 170 175
Thr Ser Gly Val Tyr Ala Gly Asp Pro Asp Gln Leu Ser Ala Leu Ala
180 185 190
Ala Phe Pro Arg Ile Ala Gly Leu Glu Glu Arg Tyr Gly Ser Leu Phe
195 200 205
Ala Gly Ala Val Gln Ala Leu Arg Ser Arg Tyr Arg Tyr Ala Thr Leu
210 215 220
Pro Arg Thr Arg His Gln Asp Ser Ala Asn Ser Pro Ile Gln Pro Pro
225 230 235 240
Pro Lys Arg Gly Gln Leu Gly Asn Leu Arg Gln Gly Leu Gln Gln Leu
245 250 255
Pro Glu Ala Ile Ala Gln Lys Leu Gly Ser Ala Leu Arg Leu Gly Trp
260 265 270
Arg Ala Val His Leu Lys Arg Asp Glu Thr Gly Tyr Arg Val Gly Phe
275 280 285
Val Ile His Asp Ser Gly Ala Glu His Thr Ala Pro Glu Glu Ile His
290 295 300
Trp Val Ala Ala Gln Gln Val Val Leu Thr Leu Pro Ala Tyr Ala Ala
305 310 315 320
Ala Thr Leu Leu Gln Asp Leu Asn Pro Gln Ala Ser Arg Leu Leu Arg
325 330 335
Glu Ile Pro Tyr Pro Pro Val Ala Val Val Ala Leu Ala Tyr Pro Glu
340 345 350
Glu Ala Leu Pro Gln Pro Leu Arg Gly Phe Gly His Leu Ile Pro Arg
355 360 365
Ser Gln Gly Leu Arg Thr Leu Gly Thr Ile Trp Ala Ser Ser Leu Phe
370 375 380
Pro Glu Arg Ala Pro Gln Gly Tyr His Cys Leu Ile Ser Phe Ile Gly
385 390 395 400
Gly Ala Thr Asp Ala Ala Phe Ala Arg Gln Lys Gly Ile Pro Pro Ile
405 410 415
Thr Ala Leu Ser Pro Asp Glu Arg Ala Gln Ile Val His Ala Glu Leu
420 425 430
Ser Gln Ile Leu Leu Thr Arg Pro Val Glu Pro Ile Arg Leu Gly Glu
435 440 445
Arg Leu Trp Pro Gln Ala Ile Pro Gln Tyr Thr Leu Gly His Arg Gln
450 455 460
Arg Ile Ala Gln Leu Gln Ala Ser Leu Ala Asp Gln Thr Pro Gly Val
465 470 475 480
Trp Val Cys Ala Asn Tyr Leu Asp Gly Val Ala Leu Gly Asp Cys Val
485 490 495
Arg Arg Ala Glu Ala Leu Ala Gln Gln Ile Leu Ser Val Arg Arg
500 505 510
<210> 48
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena biceps (WP_009629673)
<400> 48
Met Thr Ser Ala Gln Ile Pro Glu Val Asn Pro Leu Asp Val Leu Val
1 5 10 15
Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Ala Ile
20 25 30
Ala Lys Asn Tyr Arg Val Leu Val Ala Glu Ala Gln Asp Arg Val Gly
35 40 45
Gly Ala Ile Thr Ser Ala Lys Asn Asp Glu Gly Tyr Gln Trp Glu Glu
50 55 60
Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala Val
65 70 75 80
Glu Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu Pro
85 90 95
Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser Pro
100 105 110
Pro Thr Ala Ile Ala Ser Gln Ile Leu Thr Trp Gly Gly Lys Ile Arg
115 120 125
Leu Ala Leu Gly Ala Met Gly Phe Ala Arg Pro Ala Met Ala Gly Glu
130 135 140
Glu Ser Val Asp Arg Phe Phe Ser Arg Leu Leu Gly Arg Gln Ala Val
145 150 155 160
Asp Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp Pro
165 170 175
Lys Arg Leu Ser Ala Lys Ala Ala Phe Ala Lys Ile Ala Lys Leu Glu
180 185 190
Thr Tyr Gly Gly Leu Leu Ala Gly Ala Ile Leu Ser Ser Lys Glu Arg
195 200 205
Lys Ala Gln Lys Leu Asn Asp Pro Arg Ile Pro Lys Thr Lys Ala Gly
210 215 220
Glu Leu Gly Ser Phe Arg Glu Gly Ile Lys Met Leu Pro Glu Ala Ile
225 230 235 240
Thr Ala Lys Leu Arg Ala Gln Gly Thr Ala Val Lys Gln Gln Trp Thr
245 250 255
Leu Gln Ser Leu Glu Lys Gln Gly Glu Ile Tyr Val Ser Lys Phe Ala
260 265 270
Thr Pro Thr Gly Glu Glu Ile Val Thr Ser Arg Ser Val Val Leu Ser
275 280 285
Thr Pro Ala Tyr Val Thr Ala Lys Leu Leu Gln Asp Tyr Leu Pro Ala
290 295 300
Ala Ser Gln Ala Leu Asn Glu Ile Phe Tyr Pro Thr Val Ala Cys Val
305 310 315 320
Val Leu Ala Tyr Pro Lys Ser Glu Phe Arg Tyr Asp Met Lys Gly Phe
325 330 335
Gly Asn Leu Ile Pro Arg Thr Glu Gly Val Arg Thr Leu Gly Thr Ile
340 345 350
Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Ala Gly Trp Gln Leu
355 360 365
Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala His Leu
370 375 380
Ser Glu Ala Glu Ile Ile Ala Ala Val His Gln Asp Leu Lys Lys Thr
385 390 395 400
Ile Leu Arg Pro Asp Thr Lys Val Ser Pro Lys Ala Ile Ala Val His
405 410 415
Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu Glu Arg
420 425 430
Leu Ala Thr Val Glu Ala Glu Leu Gln Lys Ser Ser Gly Leu Tyr Ile
435 440 445
Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile Lys Arg
450 455 460
Ser Leu Gln Glu Ala His Lys Ile Ala Ala Phe Leu Glu Thr Ile
465 470 475
<210> 49
<211> 478
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. (PZV12410)
<400> 49
Met Thr Leu Thr Pro Ser Ser Glu Glu Asn Gln Pro Leu Asp Thr Leu
1 5 10 15
Ile Ile Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Val
20 25 30
Ile Ala Lys Asn Tyr Arg Ile Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Ser Ala Lys Asn Asp Glu Gly Tyr Leu Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Gln Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser
100 105 110
Pro Pro Thr Ala Ile Thr Ser Lys Ile Leu Thr Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Ile Gly Phe Ala Arg Pro Ala Met Ala Gly
130 135 140
Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Ile Leu Gly Lys Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Lys Ala Ala Phe Ser Lys Ile Ala Lys Leu
180 185 190
Glu Thr Tyr Gly Gly Leu Leu Ser Gly Ala Ile Leu Ser Ser Lys Glu
195 200 205
Arg Lys Ala Gln Lys Leu Asn Glu Pro Asn Ile Pro Lys Thr Lys Ala
210 215 220
Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Gln Met Leu Pro Glu Ala
225 230 235 240
Ile Ala Ser Lys Leu Arg Glu Thr Gly Thr Ala Val Lys Gln Lys Trp
245 250 255
Thr Leu Arg Ser Leu Glu Lys Gln Gly Asp Ile Tyr Ile Ser Lys Phe
260 265 270
Asp Thr Pro Ser Gly Glu Glu Thr Val Thr Ser Arg Ser Val Val Leu
275 280 285
Thr Thr Pro Ala Tyr Val Thr Ala Lys Leu Leu Glu Asp Tyr Leu Pro
290 295 300
Ala Ala Ser Gln Ala Leu Asn Glu Ile Phe Tyr Pro Thr Val Ala Cys
305 310 315 320
Val Val Leu Ala Tyr Pro Lys Ser Glu Phe Ala His Asp Met Lys Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly Thr
340 345 350
Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Glu Gly Trp Gln
355 360 365
Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala Lys
370 375 380
Leu Ser Glu Ser Glu Ile Ile Ala Ala Val His Ala Asp Leu Lys Lys
385 390 395 400
Thr Ile Leu Arg Pro Asp Thr Lys Ala Glu Pro Lys Ala Ile Ala Val
405 410 415
His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu Asp
420 425 430
Arg Leu Ala Thr Val Glu Ala Glu Leu Gln Lys Ser Gln Gly Leu Tyr
435 440 445
Val Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile Lys
450 455 460
Arg Ser Leu Gln Glu Ala Asn Lys Ile Asp Ala Phe Leu Lys
465 470 475
<210> 50
<211> 502
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. PCC 7367 (WP_015165508)
<400> 50
Met Ser Asn His Asn His Ala Gly Ser Pro Ile Asp Leu Leu Val Val
1 5 10 15
Gly Ala Gly Ile Ser Gly Leu Thr Val Ala His Asp Leu Ala Ser Asn
20 25 30
Ser Val Ala Glu Ala Asn Gly Ser Asn Gln Leu Arg Ile Ile Val Thr
35 40 45
Glu Ala Gln Asn Arg Val Gly Gly Ala Ile Val Ser Gln Arg Asp Glu
50 55 60
Val Gly Phe Gln Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro
65 70 75 80
Glu Leu Leu Lys Leu Ala Ala Ala Val Gly Leu Lys Asp Glu Leu Val
85 90 95
Phe Ala Asp Gly Lys Leu Pro Arg Phe Val Phe Trp Asn Gly Lys Leu
100 105 110
Asn Ala Leu Pro Met Ser Pro Gln Leu Leu Thr Lys Phe Asn Leu Leu
115 120 125
Thr Ile Lys Gly Lys Leu Arg Ala Phe Leu Gly Ala Ile Gly Phe Val
130 135 140
Arg Pro Ala Ala Ala Gly Glu Glu Thr Val Ala Gln Phe Phe Lys Arg
145 150 155 160
His Leu Gly Gln Glu Val Val Asp Arg Leu Val Val Pro Phe Ile Ser
165 170 175
Gly Val Tyr Ala Gly Asn Thr Asp Lys Leu Ser Ala Ala Ala Ala Phe
180 185 190
Gly Lys Ile Phe Arg Leu Glu Lys Asn Tyr Asn Gly Leu Val Ala Gly
195 200 205
Ala Ile Leu Ser Arg Leu Ala Lys Arg Lys Gln Glu Lys Ala Gln Thr
210 215 220
Asn Gln Pro Ala Asn Ile Tyr Asp Arg Gln Glu Ile Pro Lys Thr Lys
225 230 235 240
Pro Gly Gln Leu Gly Ser Phe Lys Asn Gly Ile Glu Ala Leu Pro Arg
245 250 255
Ala Ile Ala Glu Asp Leu Ile Asp Lys Gly His Glu Val Arg Leu Gln
260 265 270
Trp Arg Leu Asp Lys Ile Gln Pro Asn Ser Asp Gly Thr Tyr Ser Ala
275 280 285
Thr Tyr Glu Thr Pro His Gly Ile Glu Thr Ile Thr Ala Arg Ala Leu
290 295 300
Leu Leu Thr Thr Pro Ala Tyr Val Ser Ser Met Leu Leu Gln Asp Ile
305 310 315 320
Ala Pro Asp Ala Ala Gln Ser Leu Gly Glu Ile Tyr Tyr Pro Pro Val
325 330 335
Ala Cys Val Val Leu Gly Tyr Pro Asp Ala Ala Met Lys Arg Asp Met
340 345 350
Asn Gly Phe Gly Asn Leu Ile Pro Arg Ser Gln Gly Ile Arg Thr Leu
355 360 365
Gly Thr Ile Trp Gly Ser Ser Leu Phe Ser Asp Arg Ala Pro Ala Gly
370 375 380
Tyr His Leu Leu Leu Asn Phe Ile Gly Gly Ser Leu Asp Thr Gly Ile
385 390 395 400
Ala Asp Leu Ser Glu Pro Glu Ile Ala Gln Ala Val His Ser Asp Leu
405 410 415
Lys Gln Thr Leu Leu Lys Pro Asp Thr Thr Ile Glu Pro Lys Val Leu
420 425 430
Ala Val His Leu Trp Gln Arg Ala Ile Pro Gln Tyr Glu Val Gly His
435 440 445
Leu Asp Arg Leu Ala Arg Val Glu Arg Asp Leu Ala Asn His Pro Gly
450 455 460
Leu Phe Val Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys
465 470 475 480
Val Lys Arg Ser Phe Gly Thr Ala Glu Gln Ile Lys Gly Phe Leu Asn
485 490 495
Leu Ser Thr Lys Lys Lys
500
<210> 51
<211> 479
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. SR411 (WP_094530677)
<400> 51
Met Ser Leu Thr Pro Ser Ser Ala Thr Asn Gln Pro Leu Asp Val Leu
1 5 10 15
Val Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Ala
20 25 30
Ile Ala Lys Lys Tyr Arg Val Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Ser Ala Lys Asn Asp Glu Gly Tyr Gln Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Gln Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser
100 105 110
Pro Pro Thr Ala Ile Ala Ser Lys Ile Leu Thr Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Ile Gly Phe Ala Arg Pro Ala Met Ala Gly
130 135 140
Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Leu Leu Gly Lys Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Lys Ala Ala Phe Ala Lys Ile Phe Arg Leu
180 185 190
Glu Asn Ser Tyr Asn Gly Leu Leu Ala Gly Ala Ile Leu Ser Ala Lys
195 200 205
Glu Arg Lys Ala Gln Lys Leu Ser Asp Pro Asn Ile Pro Lys Val Lys
210 215 220
Ala Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Lys Met Leu Pro Glu
225 230 235 240
Ala Ile Ala Thr Lys Leu Arg Asp Gln Gly Thr Ala Val Lys Gln Gln
245 250 255
Trp Thr Leu Arg Ser Leu Glu Lys Gln Asp Glu Ile Tyr Ile Ser Lys
260 265 270
Phe Asp Thr Pro Thr Gly Glu Glu Thr Val Arg Ser Arg Ser Val Val
275 280 285
Leu Ser Thr Pro Ala Tyr Val Thr Ala Lys Leu Leu Gln Asp Tyr Leu
290 295 300
Pro Ala Ala Ser Gln Ala Leu Asn Glu Ile Phe Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Met Ala Tyr Pro Lys Ser Glu Phe Ala Tyr Asp Met Lys
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly
340 345 350
Thr Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Glu Gly Trp
355 360 365
Gln Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala
370 375 380
Lys Leu Ser Glu Pro Glu Ile Ile Ala Ala Val His Gln Asp Leu Lys
385 390 395 400
Lys Thr Ile Leu Arg Pro Asp Thr Lys Ala Glu Pro Lys Ala Ile Ala
405 410 415
Val His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu
420 425 430
Asp Arg Leu Ala Thr Val Glu Lys Glu Leu Gln Lys Ser Gln Gly Leu
435 440 445
Tyr Ile Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile
450 455 460
Lys Arg Ser Leu Gln Glu Ala Thr Lys Ile Asp Ala Phe Leu Lys
465 470 475
<210> 52
<211> 483
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena frigida (PZO41121)
<400> 52
Met Thr Thr Ser Gln Glu Thr Lys Ala Ser Gln Pro Leu Asp Val Leu
1 5 10 15
Val Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala His Glu Leu Ala
20 25 30
Ile Ala Lys His Arg His Val Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Thr Ala Ser Asn Asn Glu Gly Tyr Leu Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Glu Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser
100 105 110
Pro Pro Thr Ala Ile Ala Ser Lys Ile Leu Ser Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Leu Gly Phe Ala Arg Pro Ala Met Ala Gly
130 135 140
Glu Glu Ser Val Asp Arg Phe Phe Ser Arg Leu Leu Gly Lys Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Lys Ala Ala Phe Ser Lys Ile Ala Lys Leu
180 185 190
Glu Thr Tyr Gly Gly Leu Leu Ala Gly Ala Ile Leu Ser Ser Lys Glu
195 200 205
Arg Lys Ala Gln Lys Leu Asn Asp Pro Asn Ile Pro Lys Thr Lys Ala
210 215 220
Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Lys Met Leu Pro Glu Ala
225 230 235 240
Ile Ala Ala Lys Leu Arg Ala Gln Gly Thr Pro Val Lys Gln Gln Trp
245 250 255
Thr Leu Arg Ser Leu Gln Lys Gln Asp Glu Ile Tyr Ile Ala Lys Phe
260 265 270
Asp Thr Pro Thr Gly Glu Glu Val Val Thr Ser Lys Ser Val Val Leu
275 280 285
Ser Thr Pro Ala Tyr Val Ser Ala Lys Leu Leu Gln Asp Tyr Leu Pro
290 295 300
Ala Ala Ser Gln Ala Leu Ser Glu Ile Phe Tyr Pro Thr Val Ala Cys
305 310 315 320
Val Val Leu Ala Tyr Pro Lys Ser Ala Phe Ala Tyr Asp Met Lys Gly
325 330 335
Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly Thr
340 345 350
Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Asp Gly Trp Gln
355 360 365
Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala Gln
370 375 380
Leu Ser Glu Ser Glu Ile Ile Gln Ala Val His Gln Asp Leu Lys Lys
385 390 395 400
Thr Ile Leu Arg Pro Asp Thr Gln Val Glu Pro Lys Thr Ile Ala Val
405 410 415
His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu Gln
420 425 430
Arg Leu Ala Thr Ile Lys Ala Glu Leu Gln Lys Ser Gln Gly Leu Tyr
435 440 445
Ile Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys Ile Lys
450 455 460
Arg Ser Leu Gln Glu Ser Ile Glu Ile Asp Glu Phe Leu Ser Arg Thr
465 470 475 480
Asn Ser Leu
<210> 53
<211> 481
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. (PZU98053)
<400> 53
Met Asn Gln Leu Ala Asp Arg Pro Ser Gly Glu Gln Thr Leu Asp Val
1 5 10 15
Leu Val Val Gly Ala Gly Ile Ser Gly Leu Thr Ile Ala Tyr Asp Leu
20 25 30
Ala Ile Asn Gln His Arg Gln Val Leu Val Ala Glu Ala Gln Asp Arg
35 40 45
Val Gly Gly Ala Ile Val Ser Lys Gln Asn Asp Glu Gly Tyr Leu Trp
50 55 60
Glu Glu Gly Pro Asn Ser Phe Gln Pro Thr Pro Glu Leu Leu Arg Leu
65 70 75 80
Ala Val Asn Val Gly Leu Glu Asp Gln Leu Val Leu Ala Asn Gly Lys
85 90 95
Leu Pro Arg Phe Val Phe Leu Lys Gly Lys Leu Asn Ala Leu Pro Met
100 105 110
Ser Pro Pro Ala Ala Ile Ala Thr Pro Leu Leu Asp Trp Gly Ser Lys
115 120 125
Ile Arg Leu Ala Leu Gly Ala Ile Gly Phe Ala Arg Pro Ala Met Ala
130 135 140
Gly Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Leu Leu Gly Lys Gln
145 150 155 160
Ala Val Ala Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly
165 170 175
Asp Pro Lys Arg Leu Ser Ala Arg Ala Ala Phe Ala Lys Ile Phe Arg
180 185 190
Leu Glu Asn Asn Tyr Gly Gly Leu Val Ala Gly Ala Ile Leu Ser Gly
195 200 205
Lys Asp Arg Gln Ala Gln Lys Ala Lys Asn Ala Asp Leu Pro Lys Val
210 215 220
Lys Ala Gly Glu Leu Gly Ser Phe Lys Gln Gly Ile Lys Met Leu Pro
225 230 235 240
Glu Ala Ile Ala Thr Lys Leu Arg Ser Gln Gly Thr Ala Val Lys Gln
245 250 255
Gln Trp Thr Leu Arg Ser Leu Asp Arg Gln Gly Asp His Tyr Ile Ala
260 265 270
Lys Phe Asp Thr Pro Thr Gly Glu Glu Thr Val Met Ser Arg Ala Val
275 280 285
Val Leu Ala Thr Pro Ala Tyr Val Thr Ala Asn Leu Leu Lys Asp Tyr
290 295 300
Leu Pro Ser Ala Ser Gln Ala Leu Arg Lys Ile Phe Tyr Pro Thr Val
305 310 315 320
Ala Cys Val Val Leu Ala Tyr Pro Lys Thr Glu Phe Ala Tyr Asp Met
325 330 335
Gln Gly Phe Gly Asn Leu Ile Pro Arg Thr Glu Gly Val Arg Thr Leu
340 345 350
Gly Thr Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Gln Gly
355 360 365
Trp Gln Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu
370 375 380
Ala Gln Leu Ser Glu Ala Glu Ile Ile Gln Ala Val His Gln Asp Leu
385 390 395 400
Lys Lys Thr Ile Leu Arg Pro Asp Thr Lys Ala Glu Pro Lys Ala Ile
405 410 415
Ala Val His Arg Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His
420 425 430
Leu Gln Leu Leu Ala Thr Val Glu Ala Glu Leu Gln Lys Ser Gln Gly
435 440 445
Leu Tyr Ile Ser Ala Asn Phe Ile Gly Gly Val Ala Leu Gly Asp Cys
450 455 460
Ile Lys Arg Ser Leu Gln Glu Ala Ile Lys Ile Glu Gln Phe Leu Ala
465 470 475 480
Lys
<210> 54
<211> 488
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Oscillatoriales cyanobacterium CG2_30_44_21 (OIP76421)
<400> 54
Met Pro Ser Pro Gln Ser Ala Thr Asn Gln Ser Asp Asp Arg Pro Ser
1 5 10 15
Gly Gly Gln Pro Ile Asp Val Leu Val Val Gly Ala Gly Val Ser Gly
20 25 30
Leu Thr Ile Ala His Asp Leu Ala Ile Asn Tyr Gln Arg Gln Val Leu
35 40 45
Val Ala Glu Ala Gln Asp Arg Val Gly Gly Ala Ile Ile Ser Arg Gln
50 55 60
Asn Asn Glu Gly Tyr Leu Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro
65 70 75 80
Ala Pro Glu Leu Leu Arg Leu Ala Val Asn Val Gly Leu Glu Asp Gln
85 90 95
Leu Val Leu Ala Asn Gly Lys Leu Pro Arg Phe Val Phe Leu Lys Gly
100 105 110
Lys Leu Asn Ala Ile Pro Met Ser Pro Pro Ala Ala Ile Ala Thr Pro
115 120 125
Leu Leu Asp Trp Gly Ser Lys Ile Arg Leu Ala Leu Gly Ala Ile Gly
130 135 140
Phe Ala Arg Pro Ala Met Ala Gly Glu Glu Ser Val Asp Gln Phe Phe
145 150 155 160
Ser Arg Leu Leu Gly Lys Gln Ala Val Ala His Leu Val Ala Pro Phe
165 170 175
Ile Ser Gly Val Tyr Ala Gly Asp Pro Lys Arg Leu Ser Ala Arg Ala
180 185 190
Ala Phe Ala Lys Ile Phe Arg Leu Glu Asn Asn Tyr Gly Gly Leu Val
195 200 205
Ala Gly Ala Ile Leu Ser Gly Lys Asp Arg Gln Ala Gln Lys Ala Lys
210 215 220
Asn Thr Asp Leu Pro Gln Val Lys Ala Gly Glu Leu Gly Ser Phe Lys
225 230 235 240
Gln Gly Ile Lys Met Leu Pro Glu Ala Ile Ala Thr Lys Leu Arg Ser
245 250 255
Gln Gly Thr Pro Val Lys Gln Gln Trp Thr Leu Arg Ser Leu Asn Arg
260 265 270
Gln Asp Asp Ile Tyr Ile Ala Lys Phe Asp Thr Pro Thr Gly Glu Glu
275 280 285
Ile Val Met Ser Arg Ala Met Val Met Ala Thr Pro Ala Tyr Val Thr
290 295 300
Ala Asp Leu Leu Lys Asp Tyr Leu Pro Ser Ala Ser Gln Ala Leu Ser
305 310 315 320
Lys Ile Phe Tyr Pro Thr Val Ala Cys Val Val Leu Ala Tyr Pro Lys
325 330 335
Thr Glu Phe Ala Tyr Asp Met Gln Gly Phe Gly Asn Leu Ile Pro Arg
340 345 350
Thr Glu Gly Val Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu Phe
355 360 365
Ala Gly Arg Ala Pro Gln Gly Trp Gln Leu Leu Leu Asn Phe Ile Gly
370 375 380
Gly Thr Leu Asp Pro Ala Leu Ala Lys Leu Ser Asp Ser Glu Ile Ile
385 390 395 400
Gln Ala Val His Gln Asp Leu Lys Lys Thr Ile Leu Arg Pro Asp Thr
405 410 415
Lys Ala Glu Pro Lys Ala Ile Ala Val His Arg Trp Asp Lys Ala Ile
420 425 430
Pro Gln Tyr Glu Ile Gly His Leu Gln Leu Leu Ala Thr Val Glu Ala
435 440 445
Glu Leu Lys Lys Ser Gln Gly Leu Tyr Ile Ser Ala Asn Phe Ile Gly
450 455 460
Gly Val Ala Leu Gly Asp Cys Ile Lys Arg Ser Leu Gln Glu Ala Ile
465 470 475 480
Lys Ile Glu Gln Phe Leu Ala Lys
485
<210> 55
<211> 563
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Chlamydomonas reinhardtii (AF068635.1)
<400> 55
Met Met Leu Thr Gln Thr Pro Gly Thr Ala Thr Ala Ser Ser Arg Arg
1 5 10 15
Ser Gln Ile Arg Ser Ala Ala His Val Ser Ala Lys Val Ala Pro Arg
20 25 30
Pro Thr Pro Phe Ser Val Ala Ser Pro Ala Thr Ala Ala Ser Pro Ala
35 40 45
Thr Ala Ala Ala Arg Arg Thr Leu His Arg Thr Ala Ala Ala Ala Thr
50 55 60
Gly Ala Pro Thr Ala Ser Gly Ala Gly Val Ala Lys Thr Leu Asp Asn
65 70 75 80
Val Tyr Asp Val Ile Val Val Gly Gly Gly Leu Ser Gly Leu Val Thr
85 90 95
Gly Gln Ala Leu Ala Ala Gln His Lys Ile Gln Asn Phe Leu Val Thr
100 105 110
Glu Ala Arg Glu Arg Val Gly Gly Asn Ile Thr Ser Met Ser Gly Asp
115 120 125
Gly Tyr Val Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro Asn Asp Ser
130 135 140
Met Leu Gln Ile Ala Val Asp Ser Gly Cys Glu Lys Asp Leu Val Phe
145 150 155 160
Gly Asp Pro Thr Ala Pro Arg Phe Val Trp Trp Glu Gly Lys Leu Arg
165 170 175
Pro Val Pro Ser Gly Leu Asp Ala Phe Thr Phe Asp Leu Met Ser Ile
180 185 190
Pro Gly Lys Ile Arg Ala Gly Leu Gly Ala Ile Gly Leu Ile Asn Gly
195 200 205
Ala Met Pro Ser Phe Glu Glu Ser Val Glu Gln Phe Ile Arg Arg Asn
210 215 220
Leu Gly Asp Glu Val Phe Phe Arg Leu Ile Glu Pro Phe Cys Ser Gly
225 230 235 240
Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Lys Ala Ala Phe Asn
245 250 255
Arg Ile Trp Ile Leu Glu Lys Asn Gly Gly Ser Leu Val Gly Gly Ala
260 265 270
Ile Lys Leu Phe Gln Glu Arg Gln Ser Asn Pro Ala Pro Pro Arg Asp
275 280 285
Pro Arg Leu Pro Pro Lys Pro Lys Gly Gln Thr Val Gly Ser Phe Arg
290 295 300
Lys Gly Leu Lys Met Leu Pro Asp Ala Ile Glu Arg Asn Ile Pro Asp
305 310 315 320
Lys Ile Arg Val Asn Trp Lys Leu Val Ser Leu Gly Arg Glu Ala Asp
325 330 335
Gly Arg Tyr Gly Leu Val Tyr Asp Thr Pro Glu Gly Arg Val Lys Val
340 345 350
Phe Ala Arg Ala Val Ala Leu Thr Ala Pro Ser Tyr Val Val Ala Asp
355 360 365
Leu Val Lys Glu Gln Ala Pro Ala Ala Ala Glu Ala Leu Gly Ser Phe
370 375 380
Asp Tyr Pro Pro Val Gly Ala Val Thr Leu Ser Tyr Pro Leu Ser Ala
385 390 395 400
Val Arg Glu Glu Arg Lys Ala Ser Asp Gly Ser Val Pro Gly Phe Gly
405 410 415
Gln Leu His Pro Arg Thr Gln Gly Ile Thr Thr Leu Gly Thr Ile Tyr
420 425 430
Ser Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu Gly His Met Leu Leu
435 440 445
Leu Asn Tyr Ile Gly Gly Thr Thr Asn Arg Gly Ile Val Asn Gln Thr
450 455 460
Thr Glu Gln Leu Val Glu Gln Val Asp Lys Asp Leu Arg Asn Met Val
465 470 475 480
Ile Lys Pro Asp Ala Pro Lys Pro Arg Val Val Gly Val Arg Val Trp
485 490 495
Pro Arg Ala Ile Pro Gln Phe Asn Leu Gly His Leu Glu Gln Leu Asp
500 505 510
Lys Ala Arg Lys Ala Leu Asp Ala Ala Gly Leu Gln Gly Val His Leu
515 520 525
Gly Gly Asn Tyr Val Ser Gly Val Ala Leu Gly Lys Val Val Glu His
530 535 540
Gly Tyr Glu Ser Ala Ala Asn Leu Ala Lys Ser Val Ser Lys Ala Ala
545 550 555 560
Val Lys Ala
<210> 56
<211> 565
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Volvox carteri f. nagariensis hypothetical protein
(XM_002955148.1)
<400> 56
Met Leu Thr Ser His Ala Thr Asn Ile Tyr Ala Asn Ser Lys Ala Arg
1 5 10 15
Leu Gln Ile Arg Glu Ile Ala Arg Pro Thr Val Thr Gly Val Ser Arg
20 25 30
Ser Gly Val Leu Gln Asp Val Ala Ser Pro Leu Pro Arg Gln Ile Pro
35 40 45
Arg Pro Ala Gln Arg Ala Ala Arg Pro Thr Ser Ala Thr Ala Thr Ser
50 55 60
Ser Gly Val Pro Thr Ala Asp Ser Ala Arg Gly Thr Lys Ile Val Asp
65 70 75 80
Asn Val Tyr Asp Val Ile Val Val Gly Gly Gly Leu Ser Gly Leu Val
85 90 95
Thr Gly Gln Ala Leu Ser Ala Gln His Gly Val Asn Asn Phe Leu Val
100 105 110
Thr Glu Ala Arg Glu Arg Val Gly Gly Asn Ile Thr Ser Met Ser Gly
115 120 125
Asp Gly Tyr Val Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro Asn Asp
130 135 140
Ser Met Leu Gln Val Ala Val Asp Ala Gly Val Glu Lys Asp Leu Val
145 150 155 160
Leu Gly Asp Pro Lys Ala Pro Arg Phe Val Tyr Trp Gln Asn Lys Leu
165 170 175
Arg Pro Val Pro Ser Gly Pro Asp Ala Leu Thr Phe Asp Leu Met Ser
180 185 190
Leu Pro Gly Lys Ile Arg Ala Gly Leu Gly Ala Ile Gly Leu Ile Asn
195 200 205
Gly Ala Met Pro Asn Phe Glu Glu Ser Val Glu Gln Phe Ile Arg Arg
210 215 220
Asn Leu Gly Asp Glu Val Phe Glu Arg Leu Ile Glu Pro Phe Cys Ser
225 230 235 240
Gly Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Lys Ala Ala Phe
245 250 255
Asn Arg Ile Trp Ile Leu Glu Lys Asp Gly Ser Ser Leu Val Gly Gly
260 265 270
Ala Leu Lys Leu Phe Gln Glu Arg Arg Lys Asn Pro Pro Pro Pro Arg
275 280 285
Asp Pro Arg Leu Pro Pro Lys Pro Lys Gly Gln Thr Val Gly Ser Phe
290 295 300
Arg Leu Gly Leu Lys Met Leu Pro Glu Ala Ile Glu Arg Arg Ile Lys
305 310 315 320
Asp Gln Val Arg Val Asn Trp Lys Leu Val Ser Leu Thr Arg Asp Gly
325 330 335
Asp Arg Tyr Ser Leu Val Tyr Asp Thr Pro Glu Gly Arg Val Gln Cys
340 345 350
Tyr Ser Arg Ala Val Ala Leu Thr Ala Pro Ser Tyr Val Val Ala Asp
355 360 365
Leu Ile Lys Ala Glu Val Pro Ala Ala Ala Glu Ala Leu Ser Ser Phe
370 375 380
Asp Tyr Pro Pro Val Gly Ala Val Thr Leu Ser Tyr Pro Leu Ser Ala
385 390 395 400
Ile Arg Asp Asp Arg Lys Asp Ala Gln Gly Asn Val Pro Gly Phe Gly
405 410 415
Gln Leu His Pro Arg Ser Gln Gly Val Thr Thr Leu Gly Thr Ile Tyr
420 425 430
Ser Ser Ser Leu Phe Pro Gly Arg Ala Pro Pro Gly His Met Leu Leu
435 440 445
Leu Asn Tyr Ile Gly Gly Ala Thr Asn Arg Gly Ile Val Asn Gln Thr
450 455 460
Gln Glu Gln Leu Val Ala Gln Val Asp Lys Asp Leu Arg Leu Met Val
465 470 475 480
Leu Lys Pro Asp Ala Pro Ala Pro Arg Val Val Gly Val Arg Val Trp
485 490 495
Pro Arg Ala Ile Pro Gln Phe Asn Ile Gly His Leu Glu Leu Leu Asp
500 505 510
Lys Ala Arg Gly Ala Leu Glu Ala Lys Gly Trp Asn Gly Val Phe Leu
515 520 525
Gly Gly Asn Tyr Val Ser Gly Val Ala Leu Gly Lys Val Val Glu Tyr
530 535 540
Gly Tyr Glu Ser Ala Ala Lys Leu Ala Lys His Leu Thr Ala Ala Gln
545 550 555 560
Lys Gln Val Ala Val
565
<210> 57
<211> 493
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Chondrus crispus (CHC_T00000813001) (XM_005718155.1)
<400> 57
Met Ala Val Ala Glu Gly Ala Thr Pro Ala Pro Ala Ala Ala Ser Ala
1 5 10 15
Thr Pro Ser Glu Val Asp Ala Leu Val Ile Gly Ser Gly Ile Ser Gly
20 25 30
Ser Ser Leu Ala Phe Thr Leu Ser Gln Ala Ser Pro Ala Thr Ser Leu
35 40 45
Leu Leu Thr Glu Ala Arg Pro Val Val Gly Gly Asn Val Ile Ser Arg
50 55 60
Asn Glu Arg Gly Tyr Thr Trp Glu Glu Gly Pro Asn Thr Phe Gln Pro
65 70 75 80
Ala Pro His Ile Ile Arg Met Ala Val Asp Leu Gly Leu Arg Asp Asp
85 90 95
Ile Val Leu Ala Asp His Thr Leu Pro Arg Phe Val Tyr Trp Asp Gln
100 105 110
Arg Leu Phe Ala Leu Pro Leu Ser Pro Asn Asp Ile Pro Thr Phe Arg
115 120 125
Leu Leu Ser Leu Pro Gly Ala Ile Arg Ala Gly Leu Gly Ala Ala Gly
130 135 140
Phe Val Met Pro Pro Pro Lys Gly Arg Glu Glu Ser Ile Lys Asp Phe
145 150 155 160
Ile Thr Arg His Leu Gly Ala Glu Val Phe Gln Lys Met Ile Asp Pro
165 170 175
Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Ser
180 185 190
Ala Ala Phe Lys Lys Ile Tyr Ala Leu Gln Glu Leu Gly Met Thr Gln
195 200 205
Gly Ile Val Glu Gly Ala Ile Ile Arg Ile Gln Gln Lys Lys Lys Glu
210 215 220
Ala Pro Pro Pro Asp Pro Glu Leu Pro Thr Trp Lys Gly Gly Ala Leu
225 230 235 240
Gly Ser Phe Arg Lys Gly Leu Gly Met Leu Pro Gln Ala Val Ala Glu
245 250 255
Arg Leu Gly Asp Arg Val Lys Leu Ser Trp Lys Leu Val Ser Leu Gly
260 265 270
Lys Glu Ser Asp Gly Arg Tyr Arg Ala Thr Tyr Glu Thr Pro Glu Gly
275 280 285
Thr Lys Thr Val Ile Ala Arg Ser Val Ala Ile Thr Ala Pro Ala Asn
290 295 300
Ala Thr Ala Gly Ile Leu Gly Asp Leu Ala Pro Gly Ile Lys Ala Ile
305 310 315 320
Glu Glu Ile Asn Tyr Pro Thr Val Trp Ser Ile Thr Leu Ala Tyr Pro
325 330 335
Lys Asn Glu Phe Arg Glu Pro Leu Ser Gly Phe Gly Asn Leu Ile Pro
340 345 350
Arg Ser Met Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Cys Leu
355 360 365
Phe Pro Gly Arg Ala Pro Glu Gly Met Glu Leu Leu Leu Ser Tyr Ile
370 375 380
Gly Gly Ala Gln Asp Ala Ala Ile Lys Glu Leu Thr Glu Asp Glu Val
385 390 395 400
Val Ala Thr Val Asp Ala Asp Ile Lys Lys Ile Leu Met Lys Asp Gly
405 410 415
Ser Glu Val Lys Pro Val Val Val Gly Ala Arg Lys Trp Asp Arg Ala
420 425 430
Ile Pro Gln Tyr Asn Ile Gly Tyr Trp Asp Ile Met Gly Lys Ala Glu
435 440 445
Glu Ala Val Lys Glu His Pro Gly Ile Phe Leu Gly Gly Asn Tyr Thr
450 455 460
Ser Gly Val Ala Phe Gly Asp Cys Val Gln Trp Gly Ile Asp Thr Ala
465 470 475 480
Pro Lys Val Ala Glu Tyr Val Ala Ala Gln Ala Ala Val
485 490
<210> 58
<211> 570
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Galdieria sulphuraria (XM_005708373.1)
<400> 58
Met Ser Ser Gly Phe Leu Cys Phe Cys Pro Asn Ala Val Ile Lys Ala
1 5 10 15
Ala Gln Tyr Val Arg Pro Lys Asn Thr Phe Ser Cys Lys His Tyr Thr
20 25 30
Cys Lys Asn Arg Asn Ser Trp Phe Tyr Gly Asn Glu Leu Tyr Ile Lys
35 40 45
Arg Arg Lys Val Val Glu Gln Gln Arg Thr Thr Ser His Val Ser Lys
50 55 60
Leu Pro Phe Arg Gln Thr Trp Lys Ala Val Ser Ala Gln His Thr Glu
65 70 75 80
Asp Gly Glu Cys Ser Val Leu Ile Ile Gly Ser Gly Val Thr Gly Ser
85 90 95
Thr Ala Ala Phe Gln Leu Ala Glu Ser Gly Ile Asp Val Leu Val Ala
100 105 110
Glu Lys Asn Glu Gln Val Gly Gly Asn Ile Ile Ser Arg Lys Glu Gln
115 120 125
Gly Phe Ile Trp Glu Glu Gly Pro Asn Thr Phe Gln Pro Thr Pro Asp
130 135 140
Ile Leu Val Met Ile Glu Lys Leu Gly Leu Val Asp Lys Leu Val Leu
145 150 155 160
Ala Asp Ala Lys Leu Pro Arg Tyr Val Phe Tyr Gln Asp Lys Leu His
165 170 175
Lys Ile Pro Ser Ser Pro Phe Glu Ala Cys Gln Phe Ser Leu Leu Ser
180 185 190
Thr Arg Gly Lys Leu Arg Ala Phe Leu Gly Ala Ile Gly Phe Ala Pro
195 200 205
Met Asn Leu Lys Lys Lys Glu Glu Thr Val Arg Asp Phe Val Thr Arg
210 215 220
His Leu Gly Glu Glu Val Tyr Glu Arg Leu Val Asp Pro Phe Ile Ser
225 230 235 240
Gly Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Lys Ala Ala Phe
245 250 255
Lys Arg Val Gln Ala Leu Glu Glu Lys Gly Val Thr Gln Ser Leu Ile
260 265 270
Glu Gly Ala Ile Ile Arg Met Phe Glu Thr Lys Asn Ser Lys Gly Lys
275 280 285
Lys Glu Thr Lys Gly Ser Leu Lys Val Pro Arg Gly Ser Leu Gly Ser
290 295 300
Phe Lys Asp Gly Leu Gln Met Tyr Pro Gln Ser Val Gln Ser Lys Leu
305 310 315 320
Ser Asn Lys Val Lys Thr Gly Trp Lys Leu Ile Arg Leu Glu Lys Ser
325 330 335
Gly Asn Gly Ser Ser His Gln Ser Cys Glu Asp Tyr Phe Ala Val Phe
340 345 350
Gln Ile Pro Asn Gly Ile Thr Lys Val Ile Arg Thr Lys Ala Leu Ile
355 360 365
Phe Thr Ser Pro Ala Tyr Ile Thr Ala Ser Leu Leu Arg Pro Phe Ile
370 375 380
Pro Asn Ala Ser Asp Leu Leu Glu Gln Ile Tyr Tyr Pro Cys Val Val
385 390 395 400
Ser Val Ser Leu Ala Tyr Pro Ser Ser Ser Phe Arg Phe Pro Leu Ser
405 410 415
Gly Phe Gly His Leu Ile Pro Arg Ser Thr Lys Ile Arg Thr Leu Gly
420 425 430
Thr Ile Trp Ser Ser Ser Leu Phe Pro Tyr Arg Val Pro Glu Gly Tyr
435 440 445
His Leu Leu Ser Ser Tyr Ile Gly Gly Ala Gln Asp Thr Asp Ile Ala
450 455 460
Ser Leu Ser Glu Asp Gln Val Val Lys Gln Val Asp Ser Asp Ile Arg
465 470 475 480
Lys Ile Leu Leu Arg Gln Asp Ala Ala Leu Pro Lys Val Leu Gly Val
485 490 495
Arg His Trp Asn Lys Ala Ile Pro Gln Tyr Glu Leu Gly His Leu Ser
500 505 510
Arg Met Glu Thr Ile Gln Ser Gln Val Ser Glu Thr Leu Pro Gly Val
515 520 525
Phe Leu Gly Gly Asn Tyr Val Ser Gly Ile Ser Phe Gly Asp Cys Val
530 535 540
Ser Phe Gly Met Gln Leu Ala Asp Gln Ala Ser Tyr Tyr Ile Lys Glu
545 550 555 560
Asn Thr Glu Leu Leu Ser Ser Thr Val Val
565 570
<210> 59
<211> 482
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Pseudanabaena sp. ABRG5-3 (WP_126386150.1)
<400> 59
Met Thr Ser Ala Gln Pro Thr Glu Val Asn Gln Pro Leu Asp Val Leu
1 5 10 15
Val Val Gly Ala Gly Ile Ser Gly Leu Ala Ile Ala His Glu Leu Ala
20 25 30
Ile Ala Lys Asn Tyr Arg Val Leu Val Ala Glu Ala Gln Asp Arg Val
35 40 45
Gly Gly Ala Ile Thr Ser Asn Arg Asn Asp Glu Gly Tyr Leu Trp Glu
50 55 60
Glu Gly Pro Asn Ser Phe Gln Pro Ala Pro Glu Leu Leu Arg Leu Ala
65 70 75 80
Val Gln Val Gly Leu Lys Asp Glu Leu Val Leu Ala Asp Gly Lys Leu
85 90 95
Pro Arg Phe Val Phe Leu Asn Gly Lys Leu Asn Ala Leu Pro Met Ser
100 105 110
Pro Pro Thr Ala Ile Ala Ser Lys Ile Leu Thr Trp Gly Gly Lys Ile
115 120 125
Arg Leu Ala Leu Gly Ala Leu Gly Phe Ala Arg Pro Ala Met Ser Gly
130 135 140
Glu Glu Ser Val Asp Gln Phe Phe Ser Arg Leu Leu Gly Lys Gln Ala
145 150 155 160
Val Glu Arg Leu Val Ala Pro Phe Ile Ser Gly Val Tyr Ala Gly Asp
165 170 175
Pro Lys Arg Leu Ser Ala Arg Ala Ala Phe Ser Lys Ile Phe Arg Leu
180 185 190
Glu Asn Gly Tyr Gly Gly Leu Leu Ala Gly Ala Leu Leu Thr Ala Lys
195 200 205
Glu Arg Lys Ala Gln Lys Leu Asn Asp Pro Asn Ile Pro Lys Val Lys
210 215 220
Ser Gly Glu Leu Gly Ser Phe Arg Gln Gly Ile Lys Met Leu Pro Glu
225 230 235 240
Ala Ile Ala Thr Lys Leu Arg Asp Gln Gly Thr Ala Val Lys Gln Gln
245 250 255
Trp Thr Leu Arg Ser Leu Glu Lys Gln Gly Asp Ile Tyr Ile Ser Gln
260 265 270
Phe Asp Thr Pro Thr Gly Ala Glu Thr Ile Thr Ser Arg Ser Val Val
275 280 285
Leu Thr Thr Pro Ala Tyr Val Ser Ala Lys Leu Leu Gln Asp Tyr Leu
290 295 300
Pro Ala Ala Ser Gln Ala Leu Ser Glu Ile Phe Tyr Pro Thr Val Ala
305 310 315 320
Cys Val Val Leu Ala Tyr Pro Lys Ser Glu Phe Ala Tyr Asp Met Lys
325 330 335
Gly Phe Gly Asn Leu Ile Pro Arg Thr Gln Gly Val Arg Thr Leu Gly
340 345 350
Thr Ile Trp Ser Ser Ser Leu Phe Ala Gly Arg Ala Pro Asp Gly Trp
355 360 365
Gln Leu Leu Leu Asn Phe Ile Gly Gly Thr Leu Asp Pro Ala Leu Ala
370 375 380
Lys Leu Ser Glu Ala Glu Ile Val Gln Ala Val His Gln Asp Leu Lys
385 390 395 400
Lys Thr Ile Leu Arg Pro Asp Thr Lys Ala Glu Pro Lys Ala Ile Ala
405 410 415
Val His Val Trp Asp Lys Ala Ile Pro Gln Tyr Glu Ile Gly His Leu
420 425 430
Glu Arg Leu Ala Thr Ile Glu Ala Glu Leu Gln Lys Ser Gln Gly Leu
435 440 445
Tyr Val Ser Ala Asn Phe Ile Gly Gly Val Ser Leu Gly Asp Cys Ile
450 455 460
Lys Arg Gly Leu Gln Glu Ala Ser Lys Ile Asp Val Phe Leu Ser Ser
465 470 475 480
Gln Gly
<210> 60
<211> 483
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Arthrospira platensis YZ (WP_014277531.1)
<400> 60
Met Thr Asn Leu Val Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly
1 5 10 15
Leu Ser Leu Ala His Ser Leu Asn Arg Glu Lys Asn Pro Arg Ser Pro
20 25 30
Leu Lys Val Leu Val Thr Glu Ser Gln Asn Arg Val Gly Gly Asn Ile
35 40 45
Thr Thr Gly Arg Ala Ser Asp Phe Leu Trp Glu Glu Gly Pro Asn Ser
50 55 60
Phe Ala Pro Thr Pro Glu Leu Leu Gly Leu Ala Val Asp Leu Gly Leu
65 70 75 80
Lys Gln Glu Leu Ile Phe Ala Asp Arg Lys Leu Pro Arg Tyr Val Tyr
85 90 95
Trp Asn His Lys Leu His Pro Val Pro Met Thr Pro Pro Ala Leu Leu
100 105 110
Ser Ser Gln Leu Ile Ser Pro Arg Gly Lys Leu Arg Ala Ala Leu Gly
115 120 125
Ala Ile Gly Phe Val Pro Pro Pro Val Gly Ala His Leu Ser Gln Gln
130 135 140
Arg Gly Glu Glu Thr Ile Thr Gln Phe Phe His Arg His Leu Gly Ser
145 150 155 160
Glu Val Leu Glu Arg Leu Val Gln Pro Phe Val Ser Gly Val Tyr Ala
165 170 175
Gly Asp Pro Gln Gln Leu Ala Val Arg Ser Ala Phe Ser Arg Leu Val
180 185 190
Ala Ala Glu Asp Ala Gly Gly Ala Leu Leu Pro Gly Phe Val Arg Ser
195 200 205
Arg Leu Asn Lys Lys Thr Thr Lys Asp Thr Thr Pro Asp Pro Asn Ile
210 215 220
Pro Lys Thr Arg Pro Gly Glu Leu Gly Ser Phe Arg Tyr Gly Leu Glu
225 230 235 240
Thr Leu Pro Glu Thr Leu Ala Ser Lys Leu Gly Asp Arg Val Lys Leu
245 250 255
Asn Trp Thr Leu Asp Arg Phe Tyr Pro Thr Pro His Gln Thr Tyr Ile
260 265 270
Ala Glu Phe Ser Thr Pro Asp Gly Pro Gln Gln Val Glu Thr Arg Thr
275 280 285
Leu Ala Leu Met Thr Pro Ala His Val Ser Ala Arg Leu Leu Gln Pro
290 295 300
Leu His Ser Gln Ile Ala Ser Ala Leu Ser Gln Ile Pro Tyr Pro Pro
305 310 315 320
Val Ala Cys Val Val Leu Ala Tyr Pro Lys Ser Ala Leu Lys Gln Gln
325 330 335
Leu Lys Gly Phe Gly Asn Leu Ile Pro Arg His Gln Gly Ile Arg Thr
340 345 350
Leu Gly Thr Ile Trp Thr Ser Ser Leu Phe Pro Gly Arg Ala Pro Glu
355 360 365
Ser Trp Gln Val Leu Ser Asn Tyr Ile Gly Gly Ala Thr Asp Pro Glu
370 375 380
Ile Gly Glu Met Asp Asp Asp Gln Ile Val Ala Ala Val His Gln Asp
385 390 395 400
Leu Arg Gln Ile Leu Leu Ala Glu Asp Val Pro Pro Lys Val Leu Ala
405 410 415
Val His Leu Trp Arg Arg Ala Ile Pro Gln Tyr Thr Leu Gly His Gln
420 425 430
Asp Arg Leu Asn Ser Ile Asn Ala Gly Leu Arg Ser Leu Pro Gly Leu
435 440 445
Tyr Leu Cys Ser Asn Tyr Ile Asp Gly Val Ser Val Gly Asp Cys Val
450 455 460
Arg Arg Gly Gln Gln Trp Ala Ser Gln Ile Gln Ser His Leu His Pro
465 470 475 480
Thr Ala Asn
<210> 61
<211> 469
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Gloeobacter kilaueensis JS1 (WP_023175091)
<400> 61
Met Gly Arg Met Ser Val Ala Ala Glu Pro Leu Asp Val Leu Val Val
1 5 10 15
Gly Ala Gly Leu Ser Gly Leu Ala Leu Ala Trp His Leu Gln Arg Ser
20 25 30
Gly Arg Arg Val Leu Val Cys Glu Ala Thr Glu Arg Val Gly Gly Ala
35 40 45
Ile Ser Ser Glu Leu Ile Asp Gly Phe Thr Cys Glu Gly Gly Pro Asn
50 55 60
Ser Phe Gln Ser Thr Arg Pro Leu Gln Glu Leu Leu Val Glu Leu Gln
65 70 75 80
Leu Glu Glu Arg Leu Val Phe Ala Glu Glu Arg Leu Ala Arg Phe Val
85 90 95
Trp Trp Glu Asn Arg Leu Arg Pro Val Pro Met Ser Pro Ala Gln Phe
100 105 110
Val Arg Ala Asp Leu Leu Ser Trp Pro Gly Lys Leu Arg Cys Leu Ser
115 120 125
Glu Phe Phe Val Pro Pro Leu Ser Glu Pro Arg Glu Glu Thr Val Ala
130 135 140
Glu Phe Val Leu Arg Arg Phe Gly Asp Glu Ala Leu Asn Arg Leu Ile
145 150 155 160
Glu Pro Phe Ile Ala Gly Val Phe Ala Gly Asp Ala Gly Gln Leu Ser
165 170 175
Ala Asp Ala Ala Leu Ala Pro Leu Val Glu Leu Glu Arg Gln Ala Gly
180 185 190
Ser Val Leu Arg Gly Leu Trp Gln Arg Arg Asn Arg Gly Val Leu Thr
195 200 205
Pro Gln Arg Leu Cys Thr Leu Arg Gly Gly Ile Glu Gln Leu Pro Arg
210 215 220
Ala Ile Ala Arg Arg Leu Gln Ser Gln Leu Arg Phe Gln Ser Arg Leu
225 230 235 240
Glu Ala Leu Glu Pro Leu Ala Gly Gly Trp Gln Ala Ile Val Leu Asp
245 250 255
Gly Arg Gly Glu Ala Gln Ala Ile Ser Ala Arg Ser Val Ala Leu Ala
260 265 270
Ala Pro Ala Trp Ala Ile Ala Pro Val Leu Ala Arg Leu Asp Pro Ser
275 280 285
Leu Gly Arg Ala Leu Glu Ser Ile Tyr Tyr Pro Pro Val Ala Ala Val
290 295 300
Ser Leu Gly Tyr Ser Lys Ser Gln Leu Pro Asn Leu Ser Glu Gly Phe
305 310 315 320
Gly His Leu Ile Pro Arg Gly Gln Ser Leu Arg Ser Leu Gly Val Ile
325 330 335
Tyr Asn Ser Cys Leu Phe Arg His Ala Ala Pro Thr Ser Trp Arg Leu
340 345 350
Phe Thr Cys Phe Leu Gly Gly Thr Thr Asp Pro Leu Ile Ala Asp Leu
355 360 365
Ser Asp Gly Glu Leu Ala Asn Leu Ala His Arg Glu Leu Gln Ala Val
370 375 380
Leu Asp Phe Gln Ser Ser Tyr Gln Leu Leu Arg Val Ala Arg Trp Pro
385 390 395 400
Arg Ala Ile Pro Gln Tyr Ala Leu Gly His Ile Thr Lys Gln Glu Arg
405 410 415
Ile Glu Arg Tyr Leu Ala Glu Leu Pro Gly Leu Phe Leu Val Gly Asn
420 425 430
Tyr Phe Gly Gly Ile Ser Val Gly Asp Cys Val Arg Gln Ala Arg Leu
435 440 445
Lys Ala Asp Ser Val Leu Gln Phe Leu Thr Arg Thr Ala Ala Asn Gly
450 455 460
Arg Leu Asn Leu Ala
465
<210> 62
<211> 466
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Gloeobacter violaceus PCC 7421 (WP_011140945)
<400> 62
Met Asn Thr Ala Glu Glu Leu Leu Asp Val Leu Val Val Gly Ala Gly
1 5 10 15
Leu Ser Gly Leu Ala Leu Ala Trp Lys Leu Lys Arg Ala Gly Cys Thr
20 25 30
Phe Leu Val Cys Gln Ala Gly Glu Arg Val Gly Gly Ala Ile Thr Thr
35 40 45
Ala Ile Ala Asp Gly Phe Val Cys Glu Gly Gly Pro Asn Ser Phe Gln
50 55 60
Glu Ser Pro Pro Leu Ile Glu Leu Leu Thr Gln Leu Gln Leu Glu Asp
65 70 75 80
Gln Ile Val Thr Ala Asp Pro Arg Leu Ala Arg Phe Val Trp Trp Glu
85 90 95
Asn Arg Leu Arg Ser Val Pro Leu Thr Pro Ala Gln Leu Val Arg Ser
100 105 110
Asp Leu Leu Ser Trp Ser Gly Lys Ala Arg Leu Leu Trp Glu Leu Phe
115 120 125
Val Pro Ala Leu Gly Glu Pro Arg Glu Glu Thr Val Ala Glu Phe Val
130 135 140
Leu Arg Arg Phe Gly Glu Glu Val Leu Ser Arg Leu Val Asp Pro Leu
145 150 155 160
Val Ser Gly Met Cys Ala Gly Asp Val Gly Gln Leu Ser Ile Glu Ala
165 170 175
Thr Phe Glu Arg Leu Val Asp Leu Glu Arg Arg His Gly Gly Val Leu
180 185 190
Arg Gly Leu Trp Arg Thr Ala Arg Thr Arg Pro Pro Phe Lys Arg Leu
195 200 205
Cys Thr Leu Arg Gly Gly Leu Glu Gln Leu Pro Gln Ala Leu Ala Gln
210 215 220
Arg Leu Gln Pro Gln Ile Leu Leu Ser His Arg Leu Glu Ala Leu Glu
225 230 235 240
His Leu Ala Gly Asp Arg Trp Arg Ala Val Val Ala Gly Pro Gln Gly
245 250 255
Glu Pro Val Ala Ile Ala Ala Arg Thr Val Ile Leu Ser Gly Ser Ala
260 265 270
His Ala Met Ala Ser Val Leu Arg Pro Leu Asp Ala Gly Leu Gly Arg
275 280 285
Ala Leu Glu Ser Ile Tyr Tyr Pro Pro Val Val Ser Ile Ser Leu Ala
290 295 300
Tyr Ser Lys Ser Gln Val Pro Asn Ala Pro Glu Gly Phe Gly His Leu
305 310 315 320
Ile Pro Arg Asn Gln Thr Leu Arg Ser Leu Gly Val Ile Trp Asn Ser
325 330 335
Ser Leu Phe Pro His Thr Ala Pro Pro Asn Trp Arg Leu Tyr Thr Cys
340 345 350
Phe Val Gly Gly Thr Thr Asp Pro Ala Thr Pro Asn Leu Ser Asp Thr
355 360 365
Glu Leu Ala Ser Leu Ala His Arg Glu Leu Gln Thr Val Leu Gly Phe
370 375 380
Gln Ala Gly Tyr Gln Leu Leu Arg Val Thr Arg Trp Pro Gln Ala Ile
385 390 395 400
Pro Gln Tyr Ala Leu Gly His Pro Ser Lys Gln Glu Arg Val Glu Arg
405 410 415
Ala Leu Leu Gly Leu Pro Gly Leu Phe Leu Val Gly Asn Tyr Phe Gly
420 425 430
Gly Ile Ser Leu Gly Asp Cys Val Arg His Ser Gly Ala Val Ala Ser
435 440 445
Arg Val Leu Gln Phe Leu Thr Thr Val Ala Ser Asn Gly Ser Leu Arg
450 455 460
Pro Ala
465
<210> 63
<211> 538
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Panicum hallii var. hallii (PUZ57154.1)
<400> 63
Met Val Ala Ala Ala Ala Ala Ala Met Ala Thr Ala Ala Ser Ala Gly
1 5 10 15
Val Pro Pro Leu Ser Gly Pro Arg Gly Pro Ala Arg Leu Arg Ser Arg
20 25 30
Gly Val Ile Val Arg Cys Ala Ala Val Ala Gly Gly Ala Ala Glu Ala
35 40 45
Pro Thr Ser Thr Gly Ala Arg Val Ser Ala Asp Cys Val Val Val Gly
50 55 60
Gly Gly Ile Ser Gly Leu Cys Thr Ala Gln Ala Leu Ala Thr Lys His
65 70 75 80
Gly Val Gly Asp Val Leu Val Thr Glu Ala Arg Ala Arg Pro Gly Gly
85 90 95
Asn Ile Thr Thr Val Glu Arg Pro Glu Glu Gly Tyr Leu Trp Glu Glu
100 105 110
Gly Pro Asn Ser Phe Gln Pro Ser Asp Pro Val Leu Thr Met Ala Val
115 120 125
Asp Ser Gly Leu Lys Asp Asp Leu Val Phe Gly Asp Pro Asn Ala Pro
130 135 140
Arg Phe Val Leu Trp Glu Gly Lys Leu Arg Pro Val Pro Ser Lys Pro
145 150 155 160
Ala Asp Leu Pro Phe Phe Asp Leu Met Ser Ile Pro Gly Lys Leu Arg
165 170 175
Ala Gly Phe Gly Ala Leu Gly Ile Arg Pro Pro Pro Pro Gly Arg Glu
180 185 190
Glu Ser Val Glu Glu Phe Val Arg Arg Asn Leu Gly Ala Glu Val Phe
195 200 205
Glu Arg Leu Ile Glu Pro Phe Cys Ser Gly Val Tyr Ala Gly Asp Pro
210 215 220
Ser Lys Leu Ser Met Lys Ala Ala Phe Gly Lys Val Trp Arg Leu Glu
225 230 235 240
Glu Ala Gly Gly Ser Ile Ile Gly Gly Thr Ile Lys Thr Ile Gln Glu
245 250 255
Arg Gly Lys Asn Pro Lys Pro Pro Arg Asp Pro Arg Leu Pro Lys Pro
260 265 270
Lys Gly Gln Thr Val Ala Ser Phe Arg Lys Gly Leu Ala Met Leu Pro
275 280 285
Asn Ala Ile Thr Ser Ser Leu Gly Ser Lys Val Lys Leu Ser Trp Lys
290 295 300
Leu Thr Ser Ile Thr Lys Ser Asp Gly Lys Gly Tyr Val Leu Val Tyr
305 310 315 320
Glu Thr Pro Glu Gly Val Val Ser Val Gln Ala Lys Ser Val Ile Met
325 330 335
Thr Ile Pro Ser Tyr Val Ala Ser Asp Ile Leu Arg Pro Leu Ser Ser
340 345 350
Asp Ala Ala Asp Ala Leu Ser Arg Phe Tyr Tyr Pro Pro Val Ala Ala
355 360 365
Val Thr Ile Ser Tyr Pro Lys Glu Ala Ile Arg Lys Glu Cys Leu Ile
370 375 380
Asp Gly Glu Leu Gln Gly Phe Gly Gln Leu His Pro Arg Ser Gln Gly
385 390 395 400
Val Glu Thr Leu Gly Thr Ile Tyr Ser Ser Ser Leu Phe Pro Asn Arg
405 410 415
Ala Pro Ala Gly Arg Val Leu Leu Leu Asn Tyr Ile Gly Gly Ala Thr
420 425 430
Asn Thr Gly Ile Val Tyr Lys Ser Glu Ser Glu Leu Val Glu Ala Val
435 440 445
Asp Arg Asp Leu Arg Lys Met Leu Ile Asn Pro Arg Ala Val Asp Pro
450 455 460
Leu Val Leu Gly Val Arg Val Trp Pro Gln Ala Ile Pro Gln Phe Leu
465 470 475 480
Val Gly His Leu Asp Leu Leu Glu Ala Ala Lys Ser Ala Leu Gly Arg
485 490 495
Gly Gly Tyr Asp Gly Leu Phe Leu Gly Gly Asn Tyr Val Ala Gly Val
500 505 510
Ala Leu Gly Arg Cys Val Glu Gly Ala Tyr Glu Ser Ala Ser Gln Ile
515 520 525
Ser Asp Phe Leu Thr Lys Tyr Ala Tyr Lys
530 535
<210> 64
<211> 563
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Porphyra umbilicalis (OSX75961.1)
<400> 64
Met Ala Ala Phe Val Pro Ala Ala Ala Ala Ala Pro Trp Thr Ala Arg
1 5 10 15
Arg Ser Ala Phe Ala Gly Ala Pro Ala Ala Ala Gly Ser Arg Arg Pro
20 25 30
Ala Met Ala Gly Ala Ala Ala Pro Leu Arg Ser Leu Val Pro Arg Ala
35 40 45
Ile Pro Pro Pro Gly Ala Ala Arg Arg Ala Ala Pro Ser Pro Arg Met
50 55 60
Ala Ala Gly Gly Ala Pro Asp Glu Val Val Asp Ala Leu Val Val Gly
65 70 75 80
Ser Gly Val Ser Gly Ser Thr Leu Ala Tyr Arg Leu His Ser Arg Gly
85 90 95
Val Ser Met Leu Leu Thr Glu Ala Arg Asp Val Val Gly Gly Asn Val
100 105 110
Ile Ser Arg Ser Ala Asn Gly Tyr Thr Trp Glu Glu Gly Pro Asn Thr
115 120 125
Phe Gln Pro Ala Pro His Ile Leu Arg Leu Ala Val Asp Val Gly Leu
130 135 140
Lys Asp Asp Leu Val Phe Ala Asp His Thr Leu Pro Arg Phe Val Tyr
145 150 155 160
Trp Asn Gly Asn Leu Phe Ala Leu Pro Met Gly Pro Ala Asp Ile Pro
165 170 175
Lys Phe Arg Leu Leu Ser Pro Leu Gly Ala Val Arg Ala Gly Leu Gly
180 185 190
Ala Ala Gly Phe Val Trp Pro Asn Trp Ser Gly Lys Glu Glu Ser Val
195 200 205
Lys Glu Phe Ile Thr Arg His Leu Gly Ala Glu Val Phe Ala Lys Met
210 215 220
Ile Asp Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Ser Ser Leu
225 230 235 240
Ala Ile Arg Gly Ala Phe Gly Lys Ile Tyr Ala Leu Gln Asn Leu Gly
245 250 255
Ile Thr Gln Gly Ile Val Glu Gly Ala Ile Ile Arg Leu Arg Gln Arg
260 265 270
Lys Ala Glu Ala Gly Glu Pro Asp Pro Glu Leu Pro Val Val Lys Gly
275 280 285
Gly Ala Leu Gly Ser Phe Arg Glu Gly Leu Gly Met Leu Pro Lys Ala
290 295 300
Val Ala Ala Lys Leu Gly Asp Ala Val Arg Leu Gly Trp Thr Leu Thr
305 310 315 320
Glu Leu Arg Lys Leu Pro Gly Ser Ala Pro Gly Tyr Glu Ala Val Tyr
325 330 335
Ser Thr Pro Asp Gly Pro Arg Thr Val His Ala Lys Thr Val Ser Leu
340 345 350
Thr Ala Pro Ala Gly Ala Ala Ser Ser Ile Leu Gly Gly Met Leu Pro
355 360 365
Ala Ala Lys Ala Leu Asp Asp Val Tyr Tyr Pro Cys Val Trp Ser Val
370 375 380
Thr Leu Ser Tyr Pro Thr Ala Ala Phe Lys Arg Pro Leu Lys Gly Phe
385 390 395 400
Gly Asn Leu Ile Pro Arg Ser Met Gly Val Arg Thr Leu Gly Thr Ile
405 410 415
Trp Ser Ser Ser Leu Phe Pro Gly Arg Ala Pro Pro Gly Arg Glu Leu
420 425 430
Leu Leu Ser Tyr Ile Gly Gly Ala Gln Asp Lys Gly Ile Ala Asp Leu
435 440 445
Ser Glu Glu Glu Val Val Ala Ala Val Asp Gly Asp Ile Lys Ala Leu
450 455 460
Leu Leu Ser Glu Gly Ala Ala Asp Glu Val Pro Val Val Val Gly Val
465 470 475 480
Arg Lys Trp Pro Arg Ala Ile Pro Gln Tyr Val Arg Gly His Leu Glu
485 490 495
Leu Val Ala Gly Val Arg Glu Ala Ala Ala Ala Glu Cys Pro Gly Leu
500 505 510
Phe Leu Gly Gly Asn Tyr Ala Ser Gly Val Ala Phe Gly Asp Cys Val
515 520 525
Ala Trp Gly Asp Lys Ala Ala Ala Glu Val Val Ala Thr Leu Gly Asp
530 535 540
Met Pro Glu Thr Gly Ala Pro Met Ala Met Pro Glu Thr Ala Thr Pro
545 550 555 560
Ala Ser Val
<210> 65
<211> 533
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Ostreococcus tauri (XP_003079975.1)
<400> 65
Met Ser Arg Ala Gln Val Ser Leu Ala Pro Ala Arg Val Ala Asp Arg
1 5 10 15
Arg Arg Val Gly Arg Thr Arg Ala Ala Arg Arg Ala Val Ala Val Arg
20 25 30
Ala Ser Thr Val Glu Arg Asp Val Val Ile Val Gly Ala Gly Val Ser
35 40 45
Gly Leu Ser Thr Ala Phe Thr Leu Ala Lys Lys Thr Met Pro Asn Ala
50 55 60
Ser Val Met Val Thr Glu Ala Arg Asp Arg Val Gly Gly Asn Ile Thr
65 70 75 80
Ser Lys Ser Asp Gly Thr Tyr Thr Trp Glu Glu Gly Pro Asn Ser Tyr
85 90 95
Gln Pro Gly Asp Ala Ile Leu Thr Leu Ala Cys Asp Ala Gly Met Arg
100 105 110
Asp Asp Ile Leu Leu Ala Asp Pro Ala Ser Asn Arg Tyr Val Leu Trp
115 120 125
Asp Gly Lys Leu Arg Ile Leu Pro His Ser Ile Glu Ser Ala Val Leu
130 135 140
Gly Asp Phe Leu Thr Trp Pro Gly Lys Ile Arg Ala Gly Leu Gly Ala
145 150 155 160
Ile Gly Ile Arg Pro Pro Ala Pro Gly Lys Glu Glu Ser Val Lys Glu
165 170 175
Phe Val Ser Arg Asn Leu Gly Thr Glu Ala Phe Glu Arg Leu Ile Glu
180 185 190
Pro Phe Cys Ser Gly Val Tyr Ala Gly Asp Pro Ala Ser Leu Ser Ser
195 200 205
Val Ala Ala Thr Gly Arg Val Gln Arg Leu Glu Pro Leu Gly Gly Ser
210 215 220
Leu Val Val Gly Ala Leu Lys Ala Gln Ala Glu Ala Ala Lys Ala Lys
225 230 235 240
Lys Glu Ser Gly Phe Lys Arg Asp Pro Arg Leu Pro Glu Val Lys Gly
245 250 255
Gln Thr Val Gly Ser Phe Arg Gly Gly Leu Lys Thr Phe Pro Glu Gly
260 265 270
Leu Ala Lys Gln Leu Gly Asp Asp Val Val Lys Cys Asn Trp Lys Leu
275 280 285
Val Asn Val Asn Lys Ala Ala Glu Gly Gly Tyr Thr Cys Glu Tyr Asp
290 295 300
Thr Pro Glu Gly Arg Arg Thr Val Ile Ala Lys Cys Leu Leu Leu Thr
305 310 315 320
Ala Pro Ala Tyr Val Thr Ala Glu Ile Val Lys Asp Met Ala Pro Ala
325 330 335
Ala Ser Thr Ala Leu Asn Lys Phe Tyr Tyr Pro Pro Val Ala Ser Val
340 345 350
Thr Val Ser Tyr Lys Lys Asp Ser Phe Arg Leu Asp Gly Thr Ser Ala
355 360 365
Leu Pro Glu Gly Gly Leu Thr Gly Phe Gly Gln Leu His Pro Arg Ser
370 375 380
Gln Gly Ile Arg Thr Leu Gly Thr Ile Tyr Ser Ser Ser Leu Phe Lys
385 390 395 400
Asp Asp Lys Arg Gln Pro Asp Asp Glu Phe Met Ile Leu Asn Tyr Ile
405 410 415
Gly Gly Ala Arg Asp Val Ala Ile Lys Asp Leu Ser Glu Asp Glu Leu
420 425 430
Val Gln Gln Val His Glu Asp Ala Leu Lys Thr Ile Leu Lys Pro Gly
435 440 445
Thr Pro Leu Pro Lys Val Val Gly Val Lys Leu Trp Glu Lys Ala Ile
450 455 460
Pro Gln Phe Asn Leu Gly His Leu Asp Val Leu Ala Glu Ala Glu Asn
465 470 475 480
Ala Leu Glu Ala Ala Ala Cys Gly Glu Lys Asp Gly Leu Phe Leu Gly
485 490 495
Gly Asn Tyr Thr Ala Gly Val Ala Leu Gly Arg Cys Val Glu Phe Gly
500 505 510
Val Glu Gln Ala Asp Glu Val Ala Thr Phe Leu Lys Ser Ala Lys Lys
515 520 525
Leu Thr Ala Ala Val
530
<210> 66
<211> 551
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Ectocarpus siliculosus (CBJ31610.1)
<400> 66
Met Leu Ala Ala Val Gly Thr Ala Ala Gly Leu Leu Ala Val Ala Gly
1 5 10 15
Ile Thr Pro Ala Tyr Gly Phe Val Thr Pro Ser Thr Ala Trp Asn Thr
20 25 30
Ala Arg Gly Ser Gly His Gly Thr Ala Ser Arg Ala Ala Thr Thr Met
35 40 45
Ala Ala Asp Ile Phe Lys Ala Gly Pro Val Glu Lys Val Asp Cys Ala
50 55 60
Val Val Gly Ser Gly Ile Ser Gly Ser Thr Leu Gly Phe Tyr Leu Asp
65 70 75 80
Lys Lys Gly Val Asp Cys Val Val Leu Glu Ala Arg Asp Gln Val Gly
85 90 95
Gly Asn Val Ile Ser Lys Lys Glu Asp Gly Phe Leu Trp Glu Glu Gly
100 105 110
Pro Asn Ser Phe Gln Pro Thr Pro Tyr Ile Met Arg Thr Thr Val Asp
115 120 125
Leu Gly Leu Lys Glu Asp Leu Val Leu Ala Asp Pro Thr Leu Pro Arg
130 135 140
Phe Val Phe Trp Glu Gly Gly Leu Phe Pro Leu Pro Ser Ser Leu Gln
145 150 155 160
Ser Ile Ile Thr Asp Phe Trp Leu Leu Ser Trp Pro Gly Lys Ile Arg
165 170 175
Ala Gly Leu Gly Ala Ile Gly Leu Val Leu Pro Pro Pro Ser Asp Tyr
180 185 190
Glu Glu Ser Val Lys Glu Phe Val Thr Arg His Leu Gly Pro Glu Ala
195 200 205
Phe Glu Arg Leu Ile Asp Pro Phe Val Ser Gly Val Tyr Ala Gly Asp
210 215 220
Pro Ser Lys Leu Ala Ile Lys Ala Ala Leu Lys Lys Val Ala Arg Leu
225 230 235 240
Glu Val Leu Gly Gly Pro Gly Leu Ile Asp Gly Ala Ile Leu Arg Leu
245 250 255
Lys Glu Arg Ala Arg Gln Glu Lys Glu Leu Pro Glu Pro Leu Gln Ser
260 265 270
Glu Asp Leu Pro Thr Tyr Gln Gly Gly Ser Leu Gly Ser Phe Arg Glu
275 280 285
Gly Leu Gln Met Leu Pro Asn Ala Ala Leu Lys Ala Met Gly Lys Asp
290 295 300
Lys Met Arg Thr Ser Trp Val Met Lys Gly Ile Lys Arg Ser Glu Asp
305 310 315 320
Gly Gly Tyr Leu Leu Ala Phe Asp Thr Pro Lys Gly Pro Lys Arg Leu
325 330 335
Gln Ala Lys Val Ala Val Cys Thr Ala Pro Ala His Arg Leu Ala Ser
340 345 350
Val Glu Gly Leu Arg Asp Ile Val Pro Glu Ala Ala Arg Leu Asp Glu
355 360 365
Val Tyr Tyr Pro Pro Val Ala Ser Val Thr Leu Ala Tyr Pro Lys Ser
370 375 380
Ser Phe Lys Val Asp Leu Thr Gly Phe Gly Asn Leu Ile Pro Arg Lys
385 390 395 400
Met Lys Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu Phe Pro
405 410 415
Gly Arg Ala Pro Glu Gly Tyr Ala Met Leu Leu Asn Tyr Ile Gly Gly
420 425 430
Ala Gln Asp Pro Ala Ile Lys Asp Leu Ser Asp Asp Glu Ile Val Ala
435 440 445
Glu Cys Asp Arg Asp Ile Arg Thr Ile Leu Leu Lys Asp Asp Ala Pro
450 455 460
Pro Pro Lys Val Leu Gly Cys Arg Leu Trp Lys Thr Ala Ile Pro Gln
465 470 475 480
Tyr Gln Arg Gly His Leu Ala Ile Leu Glu Glu Leu Gln Glu Gly Leu
485 490 495
Lys Ala Ala Pro Gly Leu Arg Met Gly Gly Asn Tyr Ile Thr Gly Val
500 505 510
Ala Phe Gly Asp Cys Val Gln Tyr Gly Tyr Glu Glu Ala Glu Arg Ile
515 520 525
Glu Glu Met Leu Lys Ser Gly Ala Leu Asp Gly Gln Gln Ser Val Ser
530 535 540
Asp Lys Glu Ala Val Ala Ala
545 550
<210> 67
<211> 473
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Nannochloropsis gaditana CCMP526 (XM_005854685.1)
<400> 67
Met Glu Glu Thr Asp Val Leu Val Val Gly Ser Gly Ile Ser Gly Ser
1 5 10 15
Thr Thr Ala Phe Tyr Leu Asn Lys Ala Gly Val Lys Cys Leu Leu Thr
20 25 30
Glu Ala Lys Pro Val Val Gly Gly Asn Val Ile Ser Lys Ser Glu Ser
35 40 45
Gly Phe Leu Trp Glu Glu Gly Pro Asn Ser Phe Gln Pro Thr Tyr Pro
50 55 60
Leu Met Gln Ala Thr Val Asp Met Gly Leu Ala Asp Glu Leu Val Leu
65 70 75 80
Ala Asp Ala Ser Leu Pro Arg Phe Val Tyr Trp Lys Glu Lys Leu Tyr
85 90 95
Ala Leu Pro Gly Gly Leu Gly Asp Ile Pro Phe Phe Asn Leu Leu Ser
100 105 110
Ile Pro Gly Arg Ile Arg Ala Gly Leu Gly Ala Leu Gly Phe Ile Arg
115 120 125
Gly Pro Pro Lys Asp Lys Glu Glu Ser Val Lys Glu Phe Val Thr Arg
130 135 140
His Leu Gly Ala Glu Thr Phe Glu Arg Ile Ile Asp Pro Phe Val Ser
145 150 155 160
Gly Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Lys Ala Ala Leu
165 170 175
Lys Lys Val Lys Arg Leu Glu Glu Leu Gly Gly Arg Gly Ile Leu Asp
180 185 190
Gly Ala Leu Leu Arg Ile Gln Glu Ile Gln Arg Thr Lys Pro Pro Val
195 200 205
Val Pro Glu His Pro Val Tyr Lys Gly Gly Gln Leu Gly Ser Phe Lys
210 215 220
Arg Gly Leu Gln Ser Met Pro Leu Ala Ala Ala Lys Ala Leu Gly Lys
225 230 235 240
Glu Lys Val Arg Leu Ser His Lys Leu Leu Ser Val Val Glu Gly Lys
245 250 255
Gly Pro Lys Gly Gly Tyr Glu Ala Val Phe Gln Thr Pro Gln Gly Arg
260 265 270
Lys Arg Ile Lys Cys Gln Ala Leu Ala Ile Thr Ala Pro Ala His Val
275 280 285
Val His Lys Leu Leu Arg Pro Leu Val Pro Glu Ala Ala Arg Leu Ala
290 295 300
Asp Val Tyr Tyr Pro Pro Val Ala Ser Val Thr Leu Ala Tyr Pro Lys
305 310 315 320
Thr Ala Phe Arg Glu Pro Leu Arg Gly Phe Gly Asn Leu Ile Pro Arg
325 330 335
Ser Met Lys Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu Phe
340 345 350
Pro Gly Gly Pro Pro Asp Tyr Asn Met Leu Leu Ser Tyr Ile Gly Gly
355 360 365
Ala Gln Asp Pro Gly Ile Ala Glu Leu Ser Ser Gln Gln Ile Val Lys
370 375 380
Glu Val Asp Arg Asp Ile Lys Lys Val Leu Leu Lys Pro Asp Ala Pro
385 390 395 400
Ala Pro Lys Ile Leu Gly Val Arg Leu Trp Pro Thr Ala Ile Pro Gln
405 410 415
Tyr Asn Lys Gly His Leu Asp Ile Leu Ala Ser Val Glu Ala Gly Val
420 425 430
Lys Lys His Pro Gly Leu Phe Leu Gly Gly Asn Tyr Arg Thr Gly Val
435 440 445
Ala Phe Gly Asp Cys Val Thr Tyr Gly Met Glu Glu Ala Gly Arg Ile
450 455 460
Gln Ser Tyr Leu Ala Ala Lys Ala Pro
465 470
<210> 68
<211> 472
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Ostreococcus lucimarinus CCE9901 (XM_001418241.1)
<400> 68
Met Lys Lys Met Arg Glu Ala Thr Val Leu Val Thr Glu Ala Arg Glu
1 5 10 15
Arg Val Gly Gly Asn Val Thr Ser Arg Ser Asp Gly Thr Tyr Thr Trp
20 25 30
Glu Glu Gly Pro Asn Ser Tyr Gln Pro Gly Asp Ala Ile Leu Thr Leu
35 40 45
Ala Cys Asp Ala Gly Met Arg Asp Asp Ile Leu Leu Ala Asp Pro Ala
50 55 60
Ser Asn Arg Tyr Val Leu Trp Asp Gly Lys Leu Arg Ile Leu Pro His
65 70 75 80
Ser Ile Glu Ser Ala Val Leu Gly Asp Phe Leu Thr Trp Pro Gly Lys
85 90 95
Ile Arg Ala Gly Leu Gly Ala Ile Gly Ile Arg Pro Pro Ala Pro Gly
100 105 110
Lys Glu Glu Ser Val Lys Glu Phe Val Ser Arg Asn Leu Gly Thr Glu
115 120 125
Ala Phe Glu Arg Leu Ile Glu Pro Phe Cys Ser Gly Val Tyr Ala Gly
130 135 140
Asp Pro Ala Ala Leu Ser Ser Val Ala Ala Thr Gly Arg Val Gln Arg
145 150 155 160
Leu Glu Pro Leu Gly Gly Ser Leu Val Ala Gly Ala Ile Met Ala Gln
165 170 175
Lys Glu Ala Ala Gln Asn Lys Lys Pro Arg Asp Pro Arg Leu Pro Glu
180 185 190
Val Lys Gly Gln Thr Val Gly Ser Phe Arg Gly Gly Leu Lys Thr Phe
195 200 205
Pro Glu Gly Leu Ala Lys Gln Leu Gly Asp Asp Val Val Lys Cys Asn
210 215 220
Trp Lys Leu Val Gly Val Ser Lys Ser Ala Glu Gly Gly Tyr Glu Cys
225 230 235 240
Ala Tyr Asp Thr Pro Glu Gly Pro Gln Thr Val Arg Thr Lys Cys Leu
245 250 255
Leu Leu Thr Ala Pro Ala Tyr Val Ala Ala Glu Met Val Lys Asp Met
260 265 270
Ala Pro Ala Ala Ala Thr Ala Leu Asn Lys Phe Tyr Tyr Pro Pro Val
275 280 285
Ala Ser Val Thr Ile Ser Tyr Lys Lys Asp Ser Phe Arg Leu Asp Gly
290 295 300
Thr Ser Ala Leu Pro Glu Gly Gly Leu Thr Gly Phe Gly Gln Leu His
305 310 315 320
Pro Arg Ser Gln Gly Ile Arg Thr Leu Gly Thr Ile Tyr Ser Ser Ser
325 330 335
Leu Phe Lys Asp Asp Lys Arg Gln Pro Asp Asp Glu Phe Met Ile Leu
340 345 350
Asn Tyr Ile Gly Gly Ala Arg Asp Val Ala Ile Lys Asp Leu Ser Glu
355 360 365
Glu Glu Leu Val Lys Gln Val His Glu Asp Ala Leu Lys Thr Ile Leu
370 375 380
Lys Pro Gly Thr Pro Leu Pro Lys Val Val Gly Val Lys Val Trp Glu
385 390 395 400
Lys Ala Ile Pro Gln Phe Asn Leu Gly His Leu Asp Val Leu Ala Glu
405 410 415
Ala Glu Asn Ala Leu Thr Ala Ala Asp Cys Gly Glu Lys Asp Gly Leu
420 425 430
Phe Leu Gly Gly Asn Tyr Thr Ala Gly Val Ala Leu Gly Arg Cys Val
435 440 445
Glu Phe Gly Ile Glu Gln Ala Asp Glu Val Val Ala Tyr Leu Asn Ala
450 455 460
Ala Ser Lys Lys Ala Val Ala Val
465 470
<210> 69
<211> 570
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Guillardia theta CCMP2712 (XM_005821253.1)
<400> 69
Met Val Thr Met Ala Ala Val Arg Thr Lys Ala Trp Val Thr Ser Pro
1 5 10 15
Ser Met Leu Ser Ser Pro Ala Leu Leu Arg Met Pro Ser Cys Ser Trp
20 25 30
Thr Gly Lys Ser Ser Val Arg Gly Ala Arg Asp Ser Ser Val Gln Phe
35 40 45
Arg Gly Arg Ser Ala Arg Phe Gly Ala Gly Val Arg Gly Met Gln Ser
50 55 60
Ala Gly Gly Trp Gly Ala Gly Ser Met Glu Lys Tyr Asp Thr Leu Val
65 70 75 80
Ile Gly Ser Gly Val Ser Gly Ser Ser Leu Gly Phe Ser Leu Phe Gln
85 90 95
Lys Gly Val Asn Val Leu Val Thr Glu Ala Arg Asp Val Val Gly Gly
100 105 110
Asn Val Ile Thr Arg Glu Gln Asp Gly Phe Leu Trp Glu Glu Gly Pro
115 120 125
Asn Thr Phe Gln Pro Thr Arg Gln Ile Met Arg Leu Ala Val Asp Leu
130 135 140
Gly Leu Lys Asp Glu Leu Val Phe Ala Asp His Thr Leu Pro Arg Cys
145 150 155 160
Val Tyr Trp Glu Lys Glu Leu Phe Pro Leu Pro Ser Lys Pro Glu Asp
165 170 175
Ala Pro Phe Phe Arg Leu Leu Ser Ile Pro Glu Lys Ile Arg Ala Gly
180 185 190
Ile Gly Ala Ile Gly Leu His Ala Pro Lys Pro Asp Tyr Glu Glu Ser
195 200 205
Val Lys Asp Phe Ile Glu Arg His Leu Gly Glu Ala Val Phe Lys Lys
210 215 220
Met Ile Asp Pro Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Thr Lys
225 230 235 240
Leu Ser Met Ala Ser Ala Phe Lys Lys Ile Tyr Ala Leu Glu Asp Leu
245 250 255
Gly Met Thr Pro Ser Leu Ile Glu Gly Gly Ile Ile Arg Gln Ala Glu
260 265 270
Arg Ala Lys Glu Ala Arg Glu Asn Tyr Asp Pro Glu Leu Pro Thr Tyr
275 280 285
Lys Gly Gly Ala Leu Gly Ser Phe Arg Lys Gly Leu Val Ser Leu Pro
290 295 300
Lys Ala Ala Gln Glu Lys Leu Gly Asp Arg Leu Arg Thr Ser Trp Lys
305 310 315 320
Val Glu Ser Ile Ser Lys Gly Glu Asp Gly Gly Tyr Val Thr Lys Phe
325 330 335
Ser Thr Pro Gln Gly Ser Lys Glu Val Trp Ser Lys Thr Val Ala Val
340 345 350
Thr Ala Pro Ala His Ala Thr Val Gly Met Leu Ser Glu Leu Val Pro
355 360 365
Glu Cys Lys Ala Leu Glu Glu Ile His Tyr Pro Cys Val Tyr Ser Val
370 375 380
Thr Leu Ala Tyr Pro Lys Glu Cys Leu Lys Asp Glu Val Arg Lys Glu
385 390 395 400
Arg Pro Gly Leu Gly Lys Arg Leu Phe Gly Phe Gly Asn Leu Ile Pro
405 410 415
Arg Ser Met Gly Ile Arg Thr Leu Gly Thr Ile Trp Ser Ser Ser Leu
420 425 430
Phe Pro Tyr Arg Ala Pro Glu Gly Tyr Glu Met Met Leu Ser Tyr Ile
435 440 445
Gly Gly Ala Gln Asp Pro Leu Arg Tyr Asn Pro Pro Ile Ala Glu Leu
450 455 460
Ser Glu Glu Glu Val Ala Lys Ile Val His Gly Asp Val Ser Lys Ile
465 470 475 480
Leu Leu Lys Glu Gly Ala Pro Glu Pro Lys Val Leu Gly Val Arg Lys
485 490 495
Trp Pro Lys Ala Ile Pro Gln Tyr Asn Lys Gly Tyr Ser Glu Ile Met
500 505 510
Gly Lys Val Asn Ser Gly Leu Ser Lys Cys Pro Gly Leu Tyr Leu Gly
515 520 525
Gly Asn Tyr Val Ser Gly Val Ala Phe Gly Asp Cys Val Gln Trp Gly
530 535 540
Val Asp Thr Ala Pro Lys Val Lys Glu Phe Leu Asp Ser Val Pro Ser
545 550 555 560
Ser Glu Lys Ala Ala Asn Ala Ala Leu Val
565 570
<210> 70
<211> 575
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Cyanidioschyzon merolae strain 10D (XM_005535077.1)
<400> 70
Met Leu Arg Trp Ser Gly Ser Gln Gln Val Thr Asp Arg Asn Arg Ala
1 5 10 15
Glu Gln His Gly Leu Val Val Ala Phe Ala Phe Pro Ala Arg Ala Pro
20 25 30
Arg His Ser Glu Ala Thr His Glu Arg Trp Thr Gly Leu Ala Gln Arg
35 40 45
Glu Arg Gly Leu Cys Val Pro Leu Lys Ala Thr Lys Trp Lys Arg Asp
50 55 60
Leu Trp His Ser Arg Lys Leu Gln Met Gln Glu Pro Ala Gln Arg Asn
65 70 75 80
Arg Glu Gln Val Gln Ala Leu Val Val Gly Ser Gly Val Thr Gly Ser
85 90 95
Ser Phe Ala Phe Leu Leu Gln His Ala Gly Ile Ser Asp Val Ile Cys
100 105 110
Thr Glu Ala Arg Gly Glu Val Gly Gly Asn Leu Ile Ser Arg Ser Lys
115 120 125
Asp Gly Tyr Leu Trp Glu Glu Gly Pro Asn Thr Phe Gln Pro Thr Pro
130 135 140
Val Ile Leu Lys Leu Ala Arg Asp Ala Gly Leu Glu Ser Glu Leu Val
145 150 155 160
Phe Ala Asp Ala Lys Leu Pro Arg Tyr Val Tyr Trp Glu Gly Val Leu
165 170 175
His Ala Leu Pro Ser Ser Pro Ala Asp Leu Ile Thr Gly Arg Phe Arg
180 185 190
Leu Leu Ser Asn Ala Gly Lys Ala Arg Ala Ala Leu Gly Ala Leu Gly
195 200 205
Phe Val Gly Ala Pro Lys Arg Val Ala Arg Gln Ala Gly Pro Glu Pro
210 215 220
Ser Glu Asp Ala Asp Leu Ala Asp Glu Ser Val Glu Glu Phe Val Thr
225 230 235 240
Arg His Leu Gly Arg Glu Val Phe Leu Lys Ile Ile Asp Pro Phe Val
245 250 255
Ser Gly Val Tyr Ala Gly Asp Pro Ser Lys Leu Ser Met Ala Ala Ala
260 265 270
Phe Lys Arg Val Tyr Ala Leu Glu Gln Leu Gly Gly Thr Arg Gly Ile
275 280 285
Leu Glu Gly Ala Leu Ile Arg Leu Gln Gln Arg Arg Arg Glu Arg Gln
290 295 300
Arg Trp Ala Ala Glu Asn Leu Pro Lys Val Lys Ala Gly Ala Leu Gly
305 310 315 320
Ser Phe Arg Glu Gly Leu Gln Gln Leu Pro Leu Thr Val Ala Thr Arg
325 330 335
Leu Gly Pro Glu Arg Met Arg Thr Arg Tyr Ala Leu Arg Gln Ile Glu
340 345 350
Tyr Asn Gly Ala Lys Arg Gly Lys Arg Arg Tyr Thr Ala Arg Phe Glu
355 360 365
Thr Pro Asp Gly Glu Arg Val Ile Glu Thr Asp Ala Leu Ile Leu Thr
370 375 380
Ile Pro Ala His Ala Ala Ser Pro Leu Leu Gln Gly Leu Gly Val Ser
385 390 395 400
Gly Ser Glu Leu Leu Gln Glu Ile Asp Phe Pro Pro Val Tyr Ser Val
405 410 415
Thr Leu Ala Tyr Pro Lys Phe Ala Ala Arg Phe Pro Leu Asn Gly Phe
420 425 430
Gly Asn Leu Ile Pro Arg Ser Ala Gly Ile Arg Thr Leu Gly Met Val
435 440 445
Trp Ser Ser Ser Leu Phe Pro Glu Arg Ala Pro Pro Asp Met Asn Met
450 455 460
Val Leu Ser Tyr Ile Gly Gly Ala Arg Asp Pro Gly Ile Arg Glu Cys
465 470 475 480
Thr Pro Asp Glu Val Ala Ala Leu Val Asp Ala Asp Met Arg Arg Val
485 490 495
Leu Leu Arg Ala Asp Ala Pro Ser Pro Gln Val Leu Gly Val Arg Leu
500 505 510
Trp Pro Arg Ala Ile Pro Gln Tyr Asn Arg Gly His Leu Arg Arg Leu
515 520 525
Glu Ala Cys Asn Gln Gly Leu Gln Gly Phe Pro Gly Leu Phe Leu Gly
530 535 540
Gly Asn Tyr Leu Ser Gly Val Ser Phe Gly Asp Cys Val Gln Trp Ala
545 550 555 560
Tyr Asp Asn Val Pro Arg Val Leu Thr Phe Leu Gln Glu Thr Ala
565 570 575
<210> 71
<211> 559
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Bathycoccus prasinos (XM_007513261.1)
<400> 71
Met Thr Met Thr Thr Gly Ile Ser Ser Ile Gly Thr Lys Lys Asn Ser
1 5 10 15
Ile Asn Arg Ala Lys Val Ser Ser Thr Arg Arg Thr Arg Arg Val Arg
20 25 30
Ala Ala Leu Ser Ser Ser Gly Ser Lys Gly Ser Asn Phe Val Val Arg
35 40 45
Ala Ser Ser Ser Ser Ser Ser Pro Ser Asp Asp Glu Asn Asn Ser Leu
50 55 60
Gly Ile Lys Asp Thr Ile Ile Val Gly Gly Gly Val Ser Gly Leu Ser
65 70 75 80
Thr Ala Phe Thr Met Lys Ser Lys Asn Ala Ser Cys Asp Ile Met Val
85 90 95
Thr Glu Ile Arg Asp Arg Val Gly Gly Asn Val Thr Ser Lys Asn Asp
100 105 110
Gly Gln Tyr Ile Trp Glu Glu Gly Pro Asn Ser Tyr Gln Pro Gly Asp
115 120 125
Ala Val Leu Lys Leu Ala Cys Asp Ala Gly Met Lys Glu Asp Ile Val
130 135 140
Leu Ala Asn Pro Asp Ser Asp Arg Tyr Val Leu Trp Asp Gly Glu Leu
145 150 155 160
Arg Ala Leu Pro Lys Asp Ile Pro Thr Ala Val Leu Gly Asp Phe Leu
165 170 175
Thr Trp Pro Gly Lys Ile Arg Ala Gly Leu Gly Ala Val Gly Ile Arg
180 185 190
Met Pro Lys Glu Glu Gly Lys Glu Glu Thr Val Lys Glu Phe Val Ser
195 200 205
Arg Asn Leu Gly Glu Glu Ala Phe Gln Arg Leu Ile Glu Pro Phe Cys
210 215 220
Ser Gly Val Tyr Ala Gly Asp Pro Ala Met Leu Ser Ala Glu Ala Ala
225 230 235 240
Thr Gly Arg Val Ser Val Leu Glu Asn Lys Gly Pro Trp Pro Gly Leu
245 250 255
Phe Pro Gly Ala Leu Lys Ala Gln Tyr Glu Gly Ala Lys Lys Lys Lys
260 265 270
Glu Asn Pro Arg Asp Pro Arg Leu Pro Val Ile Glu Gly Gln Thr Val
275 280 285
Gly Ser Phe Lys Gly Gly Leu Gln Thr Leu Pro Glu Gly Leu Ala Lys
290 295 300
Gln Leu Gly Glu Gly Ile Val Lys Leu Gln Trp Lys Leu Ile Lys Thr
305 310 315 320
Glu Lys Thr Glu Asp Gly Leu Phe Ser Leu Thr Tyr Glu Thr Pro Glu
325 330 335
Gly Glu Lys Lys Val Lys Ala Lys Ser Val Val Phe Thr Gln Pro Ala
340 345 350
Tyr Val Val Ala Asp Thr Val Arg Ser Ile Ala Pro Glu Ala Ala Lys
355 360 365
Ser Phe Glu Lys Phe Tyr Tyr Pro Pro Val Ala Ser Val Thr Val Ala
370 375 380
Tyr Lys Arg Asp Ala Phe Lys Leu Asp Gly Arg Ser Ala Leu Pro Glu
385 390 395 400
Gly Gly Leu Thr Gly Phe Gly Gln Leu His Pro Arg Thr Gln Lys Val
405 410 415
Arg Thr Leu Gly Thr Ile Tyr Ser Ser Tyr Leu Trp Ala Asp Asp Gly
420 425 430
Arg Cys Pro Lys Asp Glu Phe Met Ile Leu Asn Tyr Ile Gly Gly Ala
435 440 445
Arg Asp Val Glu Ile Gln Lys Leu Asn Glu Asp Glu Leu Val Gln Ala
450 455 460
Val His Ala Asp Ala Leu Lys Thr Ile Leu Lys Pro Asp Thr Pro Leu
465 470 475 480
Pro Lys Lys Val Gly Val Arg Met Trp Ser Lys Ala Ile Pro Gln Phe
485 490 495
Asn Leu Gly His Trp Lys Leu Leu Asp Glu Ala Lys Glu Leu Leu Lys
500 505 510
Lys Glu Lys Cys Ser Glu Glu Asp Gly Leu Phe Leu Gly Gly Asn Tyr
515 520 525
Val Ala Gly Val Ala Phe Gly Arg Cys Val Glu Tyr Gly Val Asp Gln
530 535 540
Ala Ser Asp Val Leu Asn Phe Leu Asp Lys Gln Arg Arg Thr Val
545 550 555
<210> 72
<211> 471
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus xanthus (AY916795.1)
<400> 72
Met His His Met Pro Arg Thr Thr Gly Met Asn Val Ala Val Val Gly
1 5 10 15
Gly Gly Ile Ser Gly Leu Ala Val Ala His His Leu Arg Ser Arg Gly
20 25 30
Thr Asp Ala Val Leu Leu Glu Ser Ser Ala Arg Leu Gly Gly Ala Val
35 40 45
Gly Thr His Ala Leu Ala Gly Tyr Leu Val Glu Gln Gly Pro Asn Ser
50 55 60
Phe Leu Asp Arg Glu Pro Ala Thr Arg Ala Leu Ala Ala Ala Leu Asn
65 70 75 80
Leu Glu Gly Arg Ile Arg Ala Ala Asp Pro Ala Ala Lys Arg Arg Tyr
85 90 95
Val Tyr Thr Arg Gly Arg Leu Arg Ser Val Pro Ala Ser Pro Pro Ala
100 105 110
Phe Leu Ala Ser Asp Ile Leu Pro Leu Gly Ala Arg Leu Arg Val Ala
115 120 125
Gly Glu Leu Phe Ser Arg Arg Ala Pro Glu Gly Val Asp Glu Ser Leu
130 135 140
Ala Ala Phe Gly Arg Arg His Leu Gly His Arg Ala Thr Gln Val Leu
145 150 155 160
Leu Asp Ala Val Gln Thr Gly Ile Tyr Ala Gly Asp Val Glu Gln Leu
165 170 175
Ser Val Ala Ala Thr Phe Pro Met Leu Val Lys Met Glu Arg Glu His
180 185 190
Arg Ser Leu Ile Leu Gly Ala Ile Arg Ala Gln Lys Ala Gln Arg Gln
195 200 205
Ala Ala Leu Pro Ala Gly Thr Ala Pro Lys Leu Ser Gly Ala Leu Ser
210 215 220
Thr Phe Asp Gly Gly Leu Gln Val Leu Ile Asp Ala Leu Ala Ala Ser
225 230 235 240
Leu Gly Asp Ala Ala His Val Gly Ala Arg Val Glu Gly Leu Ala Arg
245 250 255
Glu Asp Gly Gly Trp Arg Leu Ile Ile Glu Glu His Gly Arg Arg Ala
260 265 270
Glu Leu Ser Val Ala Gln Val Val Leu Ala Ala Pro Ala His Ala Thr
275 280 285
Ala Lys Leu Leu Arg Pro Leu Asp Asp Ala Leu Ala Ala Leu Val Ala
290 295 300
Gly Ile Ala Tyr Ala Pro Ile Ala Val Val His Leu Gly Phe Asp Ala
305 310 315 320
Gly Thr Leu Pro Ala Pro Asp Gly Phe Gly Phe Leu Val Pro Ala Glu
325 330 335
Glu Gln Arg Arg Met Leu Gly Ala Ile His Ala Ser Thr Thr Phe Pro
340 345 350
Phe Arg Ala Glu Gly Gly Arg Val Leu Tyr Ser Cys Met Val Gly Gly
355 360 365
Ala Arg Gln Pro Gly Leu Val Glu Gln Asp Glu Asp Ala Leu Ala Ala
370 375 380
Leu Ala Arg Glu Glu Leu Lys Ala Leu Ala Gly Val Thr Ala Arg Pro
385 390 395 400
Ser Phe Thr Arg Val Phe Arg Trp Pro Leu Gly Ile Pro Gln Tyr Asn
405 410 415
Leu Gly His Leu Glu Arg Val Ala Ala Ile Asp Ala Ala Leu Gln Arg
420 425 430
Leu Pro Gly Leu His Leu Ile Gly Asn Ala Tyr Lys Gly Val Gly Leu
435 440 445
Asn Asp Cys Ile Arg Asn Ala Ala Gln Leu Ala Asp Ala Leu Val Ala
450 455 460
Gly Asn Thr Ser His Ala Pro
465 470
<210> 73
<211> 469
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus virescens (WP_090484749.1)
<400> 73
Met His His Met Gln Arg Thr His Arg Met Asn Val Ala Val Val Gly
1 5 10 15
Gly Gly Ile Ser Gly Leu Ala Ile Ala His Gly Leu Arg Ser Arg Gly
20 25 30
Thr Ala Ala Val Leu Leu Glu Thr Ser Ala Arg Leu Gly Gly Ala Val
35 40 45
Gly Thr His Ala Arg Ala Gly Tyr Leu Val Glu Gln Gly Pro Asn Ser
50 55 60
Phe Leu Asp Arg Glu Pro Ala Thr Arg Glu Leu Ala Ala Ala Leu Asn
65 70 75 80
Leu Glu Gly Arg Ile Arg Val Ala Asp Pro Ser Ala Lys Arg Arg Tyr
85 90 95
Val Tyr Thr Arg Gly Arg Leu Arg Ser Val Pro Ala Ser Pro Pro Ala
100 105 110
Phe Leu Ala Ser Asp Ile Leu Pro Leu Gly Ala Arg Leu Arg Val Ala
115 120 125
Gly Glu Leu Phe Ser Arg Arg Ala Pro Glu Gly Thr Asp Glu Ser Leu
130 135 140
Ala Ala Phe Gly Arg Arg His Leu Gly Arg Ala Ala Thr Arg Val Leu
145 150 155 160
Leu Asp Ala Val Gln Thr Gly Ile Tyr Ala Gly Asp Val Glu Gln Leu
165 170 175
Ser Val Glu Ala Thr Phe Pro Met Leu Val Lys Leu Glu Arg Glu His
180 185 190
Arg Ser Leu Ile Leu Gly Ala Ile Arg Ala Gln Lys Ala Gln Arg Lys
195 200 205
Ala Leu Pro Ala Gly Asp Ala Pro Lys Leu Ser Gly Ala Leu Ser Thr
210 215 220
Phe Asp Gly Gly Leu Gln Val Leu Ile Asp Ala Leu Ala Ala Ser Leu
225 230 235 240
Gly Asp Thr Ala His Val Ser Ala Arg Val Glu Gly Leu Thr Arg Val
245 250 255
Asp Gly Gly Trp Lys Leu Ala Val Glu Glu His Gly Arg Arg Ala Glu
260 265 270
Leu Thr Ala Asn His Val Val Leu Ala Val Pro Ala Phe Val Ala Ala
275 280 285
Gln Leu Leu Arg Pro Leu Asp Asp Ala Leu Ala Glu Gln Val Ser Arg
290 295 300
Ile Glu Tyr Ala Pro Ile Ala Val Val His Leu Gly Phe Asp Ala Gly
305 310 315 320
Ala Leu Pro Ala Pro Asp Gly Phe Gly Phe Leu Val Pro Phe Glu Glu
325 330 335
Lys Arg Arg Leu Leu Gly Ala Ile His Ala Ser Thr Thr Phe Pro Phe
340 345 350
Arg Val Glu Gly Gly Arg Val Leu Tyr Thr Cys Met Val Gly Gly Ala
355 360 365
Arg Gln Pro Asp Leu Val Lys Arg Asp Glu Ala Ala Leu Ala Ala Leu
370 375 380
Ala Leu Glu Glu Leu Gln Ala Leu Thr Gly Val Thr Ala Arg Pro Thr
385 390 395 400
Phe Thr Glu Val Phe Arg Trp Pro Arg Gly Ile Pro Gln Tyr Asn Val
405 410 415
Gly His Leu Ala Arg Met Ala Gly Ile Asp Ala Ala Leu Gln Arg Trp
420 425 430
Pro Gly Leu His Leu Ala Gly Asn Ala Tyr Lys Gly Ile Gly Leu Asn
435 440 445
Asp Cys Ile Arg Asn Ala Ala Arg Leu Ala Thr Ala Leu Ala Asp Glu
450 455 460
Glu Ser Val Arg Lys
465
<210> 74
<211> 463
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus macrosporus DSM 14697 (ATB45699.1)
<400> 74
Met Asn Val Ala Val Val Gly Gly Gly Ile Ser Gly Leu Ala Val Ala
1 5 10 15
His Gly Leu Arg Ser Arg Gly Thr Ala Ala Val Leu Leu Glu Thr Ser
20 25 30
Ala Arg Leu Gly Gly Ala Val Gly Thr His Ala Arg Ala Gly Tyr Leu
35 40 45
Val Glu Gln Gly Pro Asn Ser Phe Leu Asp Arg Glu Pro Ala Thr Arg
50 55 60
Ala Leu Ala Ala Ala Leu Asn Leu Glu Gly Arg Ile Arg Val Ala Asp
65 70 75 80
Ala Ser Ala Lys Arg Arg Tyr Val Tyr Thr Arg Gly Arg Leu Arg Ser
85 90 95
Val Pro Ala Ser Pro Pro Ala Phe Leu Thr Ser Asp Ile Leu Pro Leu
100 105 110
Gly Ala Arg Leu Arg Val Met Gly Glu Leu Phe Ser Ser Arg Ala Pro
115 120 125
Glu Gly Thr Asp Glu Ser Leu Ala Ala Phe Gly Arg Arg His Leu Gly
130 135 140
Pro Val Ala Thr Arg Val Leu Leu Asp Ala Val Gln Thr Gly Ile Tyr
145 150 155 160
Ala Gly Asp Val Glu Arg Leu Ser Val Glu Ala Thr Phe Pro Leu Leu
165 170 175
Val Lys Leu Glu Arg Glu His Arg Ser Leu Ile Leu Gly Ala Ile His
180 185 190
Ala Gln Lys Ala Gln Ala Arg Ala Lys Ala Leu Leu Pro Pro Gly Asp
195 200 205
Ala Pro Lys Leu Thr Gly Ala Leu Ser Thr Phe Asp Gly Gly Leu Gln
210 215 220
Val Leu Ile Asp Ala Leu Ala Ser Ser Leu Gly Asp Ala Ala His Val
225 230 235 240
Gly Ala Arg Val Glu Gly Leu Thr Arg Val Asp Gly Gly Trp Arg Leu
245 250 255
Ala Val Glu Glu His Gly Gln Arg Ala Glu Leu Ser Ala Ser His Val
260 265 270
Val Leu Ala Val Pro Ala His Val Ala Ala Glu Leu Leu Gln Pro Leu
275 280 285
Asp Asp Ala Leu Ala Ala Gln Val Ser Arg Ile Glu Tyr Ala Pro Ile
290 295 300
Ala Val Val His Leu Gly Phe Asp Ala Gly Thr Leu Pro Ala Pro Asp
305 310 315 320
Gly Phe Gly Phe Leu Val Pro Phe Glu Glu Gln Arg Arg Leu Leu Gly
325 330 335
Ala Ile His Ala Ser Thr Thr Phe Pro Phe Arg Val Glu Gly Gly Arg
340 345 350
Val Leu Tyr Thr Cys Met Val Gly Gly Ala Arg Gln Pro Asp Leu Val
355 360 365
Gln Arg Asp Glu Ala Glu Leu Ala Ala Leu Ala Leu Glu Glu Leu Arg
370 375 380
Ala Leu Ala Gly Val Thr Ala Arg Pro Thr Phe Thr Gln Val Phe Arg
385 390 395 400
Trp Pro Arg Gly Ile Pro Gln Tyr Asn Val Gly His Leu Glu Arg Met
405 410 415
Ala Gly Ile Asp Ala Ala Leu Gln Arg Trp Pro Gly Leu His Leu Ala
420 425 430
Gly Asn Ala Tyr Lys Gly Ile Gly Leu Asn Asp Cys Ile Arg Asn Ala
435 440 445
Ala Arg Leu Ala Ala Ala Leu Ser Asp Glu Glu Asn Val Arg Lys
450 455 460
<210> 75
<211> 469
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus hansupus (WP_044889345)
<400> 75
Met His His Met Pro Arg Thr His Gly Met Asn Val Ala Val Val Gly
1 5 10 15
Gly Gly Ile Ser Gly Leu Ala Val Ala His Arg Leu Arg Ser Arg Gly
20 25 30
Thr Asp Ala Val Leu Leu Glu Thr Ser Gly Arg Leu Gly Gly Ala Val
35 40 45
Gly Thr His Ala Arg Asp Gly Tyr Leu Val Glu Gln Gly Pro Asn Ser
50 55 60
Phe Leu Asp Arg Glu Pro Ala Thr Arg Glu Leu Ala Ala Ala Leu Asn
65 70 75 80
Leu Glu Gly Arg Ile Arg Val Ala Asp Pro Leu Ala Lys Arg Arg Tyr
85 90 95
Val Tyr Thr Arg Gly Arg Leu Arg Ser Val Pro Ser Ser Pro Pro Ala
100 105 110
Phe Leu Ala Ser Asp Ile Leu Pro Leu Gly Ala Arg Leu Arg Val Ala
115 120 125
Gly Glu Leu Phe Ser Gly Arg Ala Pro Thr Gly Ile Asp Glu Ser Leu
130 135 140
Ala Glu Phe Gly Arg Arg His Leu Gly Arg Thr Ala Thr Gln Val Leu
145 150 155 160
Leu Asp Ala Val Gln Thr Gly Ile Tyr Ala Gly Asp Val Glu Gln Leu
165 170 175
Ser Val Ala Ala Thr Phe Pro Met Leu Val Asp Leu Glu Arg Lys His
180 185 190
Arg Ser Leu Ile Leu Gly Ala Ile Arg Ala Gln Gln Val Gln Arg Arg
195 200 205
Ala Leu Ser Ala Gly Gly Thr Pro Lys Leu Ser Gly Ala Leu Ser Thr
210 215 220
Phe Asp Gly Gly Leu Gln Val Leu Ile Asp Ala Leu Ala Thr Ser Leu
225 230 235 240
Gly Asp Ala Ala His Val Gly Ala Gln Val Glu Cys Leu Thr Arg Val
245 250 255
Asp Gly Gly Trp Lys Leu Met Val Glu Glu Arg Gly Gln Arg Ala Glu
260 265 270
Leu Ser Ala Ser Gln Val Val Leu Ala Val Pro Ala His Val Ala Ala
275 280 285
Glu Leu Leu Arg Pro Leu Asp Asp Ala Leu Ala Ala Gln Val Ala Arg
290 295 300
Ile Asp Tyr Ala Pro Ile Ala Val Val His Leu Gly Phe Asp Ala Gly
305 310 315 320
Thr Leu Pro Ala Pro Asp Gly Phe Gly Phe Leu Val Pro Ser Gly Glu
325 330 335
Lys Arg Arg Leu Leu Gly Ala Ile His Ala Ser Thr Thr Phe Pro Phe
340 345 350
Arg Val Glu Gly Gly Arg Val Leu Tyr Thr Cys Met Val Gly Gly Ala
355 360 365
Arg Gln Pro Glu Leu Val Arg Gln Asp Glu Ala Ala Leu Ala Ala Leu
370 375 380
Ala Leu Glu Glu Leu Arg Ala Leu Ala Gly Val Thr Ala Gln Pro Thr
385 390 395 400
Phe Thr Gln Val Tyr Arg Trp Gln Arg Gly Ile Pro Gln Tyr Asn Val
405 410 415
Gly His Leu Glu Arg Met Ala Gly Ile Asp Ala Ala Leu Gln Arg Trp
420 425 430
Pro Gly Leu His Leu Ala Gly Asn Ala Tyr Lys Gly Ile Gly Leu Asn
435 440 445
Asp Cys Ile Arg Asn Ala Ala Arg Leu Ala Asp Ala Leu Thr Asp Glu
450 455 460
Glu Ser Val Arg Lys
465
<210> 76
<211> 461
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus fulvus (WP_046711394.1)
<400> 76
Met Thr Val Ala Val Val Gly Gly Gly Ile Ser Gly Leu Val Val Ala
1 5 10 15
Trp Arg Leu Arg Ser Arg Gly Arg Asp Ala Val Leu Leu Glu Thr Thr
20 25 30
Ser Arg Leu Gly Gly Ala Val Gly Thr Arg Ala Gln Arg Gly Phe Leu
35 40 45
Leu Glu Thr Gly Pro Asn Ser Phe Leu Asp Arg Glu Pro Ala Met Arg
50 55 60
Glu Leu Ala Ser Ala Leu Asn Leu Glu His Arg Ile Arg Ala Ala Asp
65 70 75 80
Gly Ala Ala Lys Arg Arg Tyr Val Tyr Thr Arg Gly Lys Leu Arg Ser
85 90 95
Val Pro Ala Ser Pro Pro Ala Phe Leu Lys Ser Asp Ile Leu Pro Phe
100 105 110
Gly Ala Lys Leu Arg Val Met Gly Glu Leu Phe Ser Gly Arg Ala Ala
115 120 125
Pro Gly Val Asp Glu Ser Leu Ala Asp Phe Gly Arg Arg His Leu Gly
130 135 140
Ala Thr Ala Thr Arg Val Leu Leu Asp Ala Val Gln Thr Gly Ile Phe
145 150 155 160
Ala Gly Asp Val Glu Lys Leu Ser Val Gly Ala Thr Phe Pro Pro Leu
165 170 175
Val Lys Leu Glu Arg Glu His Arg Ser Leu Leu Leu Gly Ala Ile Gln
180 185 190
Ala Gln Lys Ala Gln Lys Ala Arg Ala Lys Ala Leu Pro Ala Gly Ser
195 200 205
Thr Ala Pro Lys Leu Ser Gly Ala Leu Ser Thr Phe Glu Gly Gly Leu
210 215 220
Gly Thr Leu Ile Asp Ala Leu Gly Thr Ala Leu Gly Asp Ala Ala Arg
225 230 235 240
Thr Gly Ala Thr Val Glu Gly Leu Thr Arg Gly Asp Asp Gly Trp Arg
245 250 255
Leu Ala Val Ser Glu Arg Gly Gln Arg Ser Glu Leu Lys Ala Ser Ser
260 265 270
Val Val Leu Ala Ala Pro Ala Tyr Val Thr Arg Gly Leu Leu Glu Pro
275 280 285
Leu Asp Ala Glu Leu Ala Ala Arg Val Gly Gly Ile Asp Tyr Ala Pro
290 295 300
Ile Ala Val Val His Leu Gly Phe Asp Ala Gly Thr Thr Pro Ala Pro
305 310 315 320
Asp Gly Phe Gly Phe Leu Val Pro Pro Met Glu Lys Arg Arg Leu Leu
325 330 335
Gly Ala Ile His Ala Ser Thr Val Phe Pro Phe Arg Val Glu Ala Gly
340 345 350
Arg Val Leu Tyr Thr Cys Met Val Gly Gly Ala Thr Arg Pro Asp Leu
355 360 365
Val Ala Leu Asp Glu Ala Glu Leu Val Ala Leu Ala Arg Glu Glu Leu
370 375 380
Lys Ala Leu Ala Gly Val Thr Ala Thr Pro Thr Leu Thr Glu Val Phe
385 390 395 400
Arg Trp Lys Arg Gly Ile Pro Gln Tyr Asn Leu Gly His Leu Glu Arg
405 410 415
Met Asp Gly Val Asp Arg Ala Leu Thr Arg Leu Pro Gly Leu His Leu
420 425 430
Ala Gly Asn Ala Tyr Lys Gly Val Gly Leu Asn Asp Cys Ile Arg Asn
435 440 445
Gly Leu Ala Leu Ala Asp Ala Leu Val Asp Ala Gly Ala
450 455 460
<210> 77
<211> 461
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus fulvus (WP_074949681.1)
<400> 77
Met Thr Val Ala Val Val Gly Gly Gly Ile Ser Gly Leu Val Val Ala
1 5 10 15
Trp Arg Leu Arg Ser Arg Gly Arg Asp Ala Val Leu Leu Glu Thr Thr
20 25 30
Ser Arg Leu Gly Gly Ala Val Gly Thr Arg Ala Gln Arg Gly Phe Leu
35 40 45
Leu Glu Thr Gly Pro Asn Ser Phe Leu Asp Arg Glu Pro Ala Met Arg
50 55 60
Glu Leu Ala Ser Ala Leu Asn Leu Glu His Arg Ile Arg Ala Ala Asp
65 70 75 80
Gly Ala Ala Lys Arg Arg Tyr Val Tyr Thr Arg Gly Lys Leu Arg Ser
85 90 95
Val Pro Ala Ser Pro Pro Ala Phe Leu Lys Ser Asp Ile Leu Pro Phe
100 105 110
Gly Ala Lys Leu Arg Val Met Gly Glu Leu Phe Ser Gly Arg Ala Ala
115 120 125
Pro Gly Val Asp Glu Ser Leu Ala Asp Phe Gly Arg Arg His Leu Gly
130 135 140
Ala Thr Ala Thr Arg Val Leu Leu Asp Ala Val Gln Thr Gly Ile Phe
145 150 155 160
Ala Gly Asp Val Glu Lys Leu Ser Val Gly Ala Thr Phe Pro Pro Leu
165 170 175
Val Lys Leu Glu Arg Glu His Arg Ser Leu Leu Leu Gly Ala Ile Gln
180 185 190
Ala Gln Lys Ala Gln Lys Ala Arg Ala Lys Ala Leu Pro Ala Gly Ser
195 200 205
Thr Gly Pro Lys Leu Ser Gly Ala Leu Ser Thr Phe Glu Gly Gly Leu
210 215 220
Gly Thr Leu Ile Asp Ala Leu Gly Thr Ala Leu Gly Asp Ala Ala Arg
225 230 235 240
Thr Gly Ala Thr Val Glu Gly Leu Thr Arg Gly Asp Asp Gly Trp Arg
245 250 255
Leu Ala Val Ser Glu Arg Gly Gln Arg Ser Glu Leu Lys Ala Ser Ser
260 265 270
Val Val Leu Ala Ala Pro Ala Tyr Val Thr Arg Gly Leu Leu Glu Pro
275 280 285
Leu Asp Ala Glu Leu Ala Ala Arg Val Gly Gly Ile Asp Tyr Ala Pro
290 295 300
Ile Ala Val Val His Leu Gly Phe Asp Ala Gly Thr Thr Pro Ala Pro
305 310 315 320
Asp Gly Phe Gly Phe Leu Val Pro Pro Met Glu Lys Arg Arg Leu Leu
325 330 335
Gly Ala Ile His Ala Ser Thr Val Phe Pro Phe Arg Val Glu Ala Gly
340 345 350
Arg Val Leu Tyr Thr Cys Met Val Gly Gly Ala Thr Arg Pro Asp Leu
355 360 365
Val Ala Leu Asp Glu Ala Glu Leu Val Ala Leu Ala Arg Glu Glu Leu
370 375 380
Lys Ala Leu Ala Gly Val Thr Ala Thr Pro Thr Leu Thr Glu Val Phe
385 390 395 400
Arg Trp Lys Arg Gly Ile Pro Gln Tyr Asn Leu Gly His Leu Glu Arg
405 410 415
Met Asp Gly Val Asp Arg Ala Leu Thr Arg Leu Pro Gly Leu His Leu
420 425 430
Ala Gly Asn Ala Tyr Lys Gly Val Gly Leu Asn Asp Cys Ile Arg Asn
435 440 445
Gly Leu Ala Leu Ala Asp Ala Leu Val Asp Ala Gly Ala
450 455 460
<210> 78
<211> 459
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Myxococcus stipitatus (WP_015346914.1)
<400> 78
Met Thr Val Ala Val Val Gly Gly Gly Ile Ser Gly Leu Val Val Ala
1 5 10 15
Trp Arg Leu Arg Ser Arg Gly Lys Ala Ala Val Val Leu Glu Thr Thr
20 25 30
Ser Arg Leu Gly Gly Ala Val Gly Thr Arg Ala Lys Gln Gly Phe Leu
35 40 45
Leu Glu Thr Gly Pro Asn Ser Phe Leu Asp Arg Glu Pro Ala Thr Arg
50 55 60
Glu Leu Ala Gly Ala Leu Asn Leu Glu His Arg Ile Arg Ala Ala Asp
65 70 75 80
Ala Ala Ala Lys Arg Arg Tyr Val Tyr Thr Arg Gly Gln Leu Arg Ser
85 90 95
Val Pro Ala Ser Pro Pro Ala Phe Leu Gly Ser Asp Ile Leu Pro Trp
100 105 110
Ser Ala Lys Leu Arg Val Met Gly Glu Leu Phe Thr Gly Arg Ala Ala
115 120 125
Pro Gly Ile Asp Glu Ser Leu Ala Ala Phe Gly Arg Arg His Leu Gly
130 135 140
Ala Thr Ala Thr Arg Val Leu Leu Asp Ala Val Gln Thr Gly Ile Phe
145 150 155 160
Ala Gly Asp Val Glu Arg Leu Ser Val Gly Ala Thr Phe Pro Pro Leu
165 170 175
Val Lys Leu Glu His Glu His Arg Ser Leu Ile Leu Gly Ala Ile Arg
180 185 190
Thr Gln Gln Ala Arg Arg Lys Ala Leu Pro Ala Gly Ala Ser Ala Ala
195 200 205
Pro Glu Leu Ser Gly Ala Leu Ser Thr Phe Glu Gly Gly Leu Gln Thr
210 215 220
Leu Ile Asp Ala Leu Ser Ala Ser Leu Gly Glu Asp Ala Arg Val Asn
225 230 235 240
Ala Thr Val Glu Gly Leu Thr Arg Val Gly Asp Gly Trp Arg Leu Ala
245 250 255
Val Ser Glu Lys Gly Gln Arg Ser Glu Leu Asp Ala Ser His Val Val
260 265 270
Leu Ala Ala Pro Ala Tyr Val Thr Gln Lys Leu Leu His Pro Leu Asp
275 280 285
Ala Glu Leu Ala Ala Arg Val Gly Gly Ile Glu Tyr Ala Pro Ile Ala
290 295 300
Val Val Gln Leu Gly Phe Asp Val Gly Thr Thr Pro Ala Pro Asp Gly
305 310 315 320
Phe Gly Phe Leu Val Pro Pro Ser Glu Gly Arg Arg Leu Leu Gly Ser
325 330 335
Ile His Ala Ser Thr Val Phe Pro Phe Arg Val Glu Pro Gly Arg Val
340 345 350
Leu Tyr Thr Cys Met Val Gly Gly Ala Lys Arg Pro Asp Leu Val Gly
355 360 365
Leu Asp Glu Pro Ala Leu Val Ala Leu Ala Arg Glu Glu Leu Lys Ala
370 375 380
Leu Ala Gly Val Thr Ala Thr Pro Ser Leu Thr Glu Val Phe Arg Trp
385 390 395 400
Pro Leu Gly Ile Pro Gln Tyr Asn Val Gly His Leu Ala Arg Met Ala
405 410 415
Ala Val Asp Gln Ala Leu Thr Arg Arg Pro Gly Leu Ser Leu Thr Gly
420 425 430
Asn Ala Tyr Lys Gly Val Gly Leu Asn Asp Cys Ile Arg Asn Gly Leu
435 440 445
Gln Leu Ala Asp Ala Leu Val Thr Ala Ala Gly
450 455
<210> 79
<211> 458
<212> PRT
<213> Artificial Sequence
<220>
<223> PPO of Hyalangium minutum (WP_044193071.1)
<400> 79
Met Thr Val Ile Ala Val Ile Gly Gly Gly Ile Ser Gly Leu Thr Leu
1 5 10 15
Thr His Cys Leu Arg Ser Arg Gly Lys Asp Ala Leu Leu Leu Glu Ala
20 25 30
Ser Ser Arg Leu Gly Gly Asn Ile Glu Thr Arg Gln Arg Asp Gly Phe
35 40 45
Leu Ile Glu Thr Gly Pro Asn Ser Phe Leu Asp Arg Glu Pro Ala Thr
50 55 60
Arg Glu Leu Ala Ala Gly Val Gly Val Glu Asp Arg Ile Arg Ser Ala
65 70 75 80
Asp Pro Ala Ala Lys Ala Arg Tyr Leu Tyr Thr Arg Gly Arg Leu Arg
85 90 95
Pro Val Pro Ser Ser Pro Pro Ala Phe Leu Lys Ser Asp Ile Leu Pro
100 105 110
Leu Gly Ala Arg Leu Arg Val Met Ala Glu Leu Phe Thr Gly Arg Ala
115 120 125
Pro Glu Gly Val Asp Glu Ser Leu Ala Ala Phe Gly Arg Arg His Leu
130 135 140
Gly Pro Ala Ala Thr Ala Val Leu Leu Asp Ala Val Gln Thr Gly Ile
145 150 155 160
Tyr Ala Gly Asn Met Glu Thr Leu Ser Val Asp Ala Thr Phe Pro Gln
165 170 175
Leu Thr Lys Leu Glu Arg Glu His Arg Ser Leu Ile Leu Gly Ala Ile
180 185 190
Arg Ser Gln Lys Ala Gln Arg Lys Ala Leu Pro Ala Gly Ala Ala Gly
195 200 205
Ser Pro Glu Lys Leu Arg Gly Thr Leu Cys Thr Phe Asp Gly Gly Leu
210 215 220
Gln Thr Leu Val Asp Gly Leu Ala Arg Glu Leu Gly Pro Ala Ala His
225 230 235 240
Thr Asn Ala Lys Val Glu Gly Leu Gln Pro Ser His Gly Gly Trp Arg
245 250 255
Val Ser Val Arg Glu Asn Gly Gly Gln Ala Glu Leu Leu Ala Ser Gln
260 265 270
Val Val Leu Ala Thr Pro Ala Phe Val Ala Ala Gly Leu Met Arg Pro
275 280 285
Leu Asp Glu Pro Leu Ala Ala Leu Val Glu Gly Ile Ala Tyr Ala Pro
290 295 300
Ile Ala Val Val His Leu Gly Phe Ala Pro Gly Ser Thr Pro Ala Pro
305 310 315 320
Asp Gly Phe Gly Phe Leu Val Pro Gly Leu Glu Lys Arg Arg Leu Leu
325 330 335
Gly Ala Ile His Ala Ser Thr Val Phe Pro Phe Arg Thr Glu Gly Gly
340 345 350
Arg Val Leu Tyr Thr Cys Met Val Gly Gly Ala Arg Gln Pro Asp Leu
355 360 365
Val Lys Leu Asp Glu Glu Ala Leu Val Ala Leu Ala Arg Glu Glu Leu
370 375 380
Lys Glu Leu Ala Gly Val Thr Ala Ser Pro Ser Phe Thr Glu Val Ile
385 390 395 400
Arg Trp Thr Arg Gly Ile Pro Gln Tyr Asn Val Gly His Leu Glu Arg
405 410 415
Val Ala Ala Ile Asp Ala Ala Leu Lys Arg Trp Pro Gly Leu His Leu
420 425 430
Thr Gly Asn Ala Tyr Lys Gly Val Gly Ile Asn Asp Cys Ile Arg Asn
435 440 445
Ala Phe Ala Leu Gly Asp Ala Leu Ala Ala
450 455
<210> 80
<211> 1404
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 1
<400> 80
atgagtgagg tagatgtcgc aattgtcggt ggtggcctca gtggcctgag tctggcttgg 60
cggttgcagc aaagcgcccc ccagtacagc gtggttcttt tagaggccag cgatcgcctt 120
gggggcaata ttacgacgca aacagcagag ggctttgtct gggaactagg tcccaatagt 180
tttgcaccga cccccgccct gttgcaactc attgctgagg tgggcttgca gtcggaactc 240
attcggggcg atcgccacct gccgcgctac atctactggc ggggacaact ctacccccta 300
caacccaccc gtcctcttgc cttagccacc tcaaacctcc tcagtccttg gggaaaagtg 360
cgggcagccc ttggtgccct tgggtttgtg cccccctatt taggcagcgg cgatgagtca 420
gtcaattcat ttttccgtcg ccatttgggg caagaggtag ctgaacgact ggtggcgccc 480
tttgtctctg gggtttatgc gggtgatcca caacaactga gtgctgctgc tgcttttcgc 540
cgtattgctc aattggaaaa acttgggggt ggcctcattg ctggtgccct gcacctacgg 600
cgtcaacaag cccccaaacc caagccgcct acaagtgtgc agatgcgacc gggggaactt 660
ggttccttta aggaaggcct agccgcgcta cctcgggcga tcgcccaaca actgaaggca 720
ccgattcacc tgcaaacccc tgttcaagaa atcaccccag accctaaggg tgggtacctg 780
ctgcgcagtg gtgagcaaac gtggcgcgcc cgcagtgtgg ttttggcaac gcctgcctat 840
caaaccgcag agttagtggc accctttcag ccagcgatcg cccgtgtttt ggccaccatt 900
ccctatccca ccgtggcctg tgtggtcttg gcctatcctg ctggacttgg gcgcagtgtc 960
cgccccggct ttggcgtact gattcctcgc agtcagggca tccgcaccct tggcaccatt 1020
tggtcgtcct gtctctttcc ccaacgcacc cccgccggtt ggcaagtctt tacgagtttt 1080
attggcggtg ctacggatcc tgatttggca agcctgagcg aagaagccat tgttcagcag 1140
gtgcagcagg atttgaaccg actccttgat ttacccgcag ccaaggcacg cttactcggt 1200
atgaaggttt ggcgacgggc gattccccaa tatatggtgg gctaccctga gcagtggcag 1260
caggtgaccc atgccctcag tcaaaccccc ggccttttcc tgtgtagtaa ttatgctgag 1320
ggtgtcgcct tgggcgatcg cgtcgaacat ggcaatcgaa ccgctgctgc tgttgctgcc 1380
tacctttcag gaggccagcc ctag 1404
<210> 81
<211> 1398
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 2
<400> 81
atgatggagg tagatgtcgc aattgttggg ggtggtctca gtggcctgag tgttgcttgg 60
cggttgcagc agagcgcccc gcagtacagt ggggttcttt tagaggctag cgatcgcctg 120
ggggggaata tcacgaccca aggggcggag ggttttgtct gggaactggg tcccaatagc 180
tttgcaccca cccctgccct cctacaactc attgctgagg tgggcttgca ctcagagcta 240
attcggggcg atcgccacct accgcgctac atctactggc ggggggaact ctaccccctc 300
gaacccaccc gtccccttgc tttggccacc tcaaatttac tcagcccttg gggaaaagtg 360
cgggcagccc ttggtgcctt ggggtttgtg cccccctatc tgggcagtgg ggatgagtcg 420
gttaattcat ttttccgtcg ccatttgggg caagaggtgg ctgaacgact ggtggccccc 480
tttgtctctg gggtttacgc cggtgatccg caacaattga gtgccgctgc tgcttttcgt 540
cgtattgccc aactggaaaa actagggggt gggctcattg ccggcgccct gcgcctgcgg 600
cgccaacaac ctcctaaacc aaggccacct gcagaggtgc aaatgcgacc aggggaactg 660
ggctccttta aggaaggctt ggctgctctg ccgcgggcga tcgcccaaca attgaaggca 720
ccggttcacc tgcaaacccc cgttgaagca atcaccccag aacccaatgg tggctatctg 780
ctgcgcagtg gtgagcaaac gtggcaggcc cgcagtgtgg tcttggcaac gcctgcctat 840
caaaccgcgg cgttggtagc gccctttcag ccagcgatcg cccgtgtttt ggctgccatc 900
ccctatccca ccgtggcctg tgtggtcttg gcctaccccg cgggactggg acgcagtgta 960
cgccccggat ttggcgtact gattcctcgt ggccagggca tccgtaccct tggcaccatt 1020
tggtcgtcct gtctttttcc ccaacgcacc cccgccggct ggcaagtctt tacaagtttt 1080
attggcggtg ccacggatcc tgatttggct agccttaggg aagaagccat tgtccagcaa 1140
gtgcagcagg atttgacccg cctccttgat ttacccgccg ccaaggcacg attactcggt 1200
atgaaagttt ggcgacgggc gattccccaa tatcttgtgg gctaccctca gcagtggcag 1260
caggtgaccc atgccctcag ccacacccct gggcttttcc tgtgtagtaa ctatgcggag 1320
ggggtggcct tgggcgatcg cgtcgaacat ggcaatcgaa ccgctgctgc tgttgctgcc 1380
tacctttcag gaggttaa 1398
<210> 82
<211> 1404
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 3
<400> 82
atgattgagg tagatgtcgc aattgttggg ggtggtctca gtggcctcag tgttgcttgg 60
cggttgcagc ggagcgcccc tcactacagt ggcgttcttt tagaggccag cgatcgcctg 120
gggggcaata tcacgaccca agcggcggag ggctttgtct gggaactggg tcccaatagc 180
tttgccccca cccctgccct cctacaactc attgccgagg tgggcttgca ctcagagctc 240
attcggggcg atcgccacct accgcgctac atctactggc ggggggaact ctaccccctc 300
gaacccaccc gtcccctggc cttggccact tcaaatttgc tcagcccttg gggaaaagta 360
cgggcagccc ttggtgcctt ggggtttgtg cctccctatc tgggcagtgg ggacgagtca 420
gttgattcat ttttccggcg ccatttgggg caagaggttg ctgaacgact agtggccccc 480
tttgtctctg gggtttatgc cggtgatccg caacaattga gtgccgctgc cgcttttcgt 540
cgtattgccc aactggaaaa actagggggt agcctcattg ccggggctct gcgcctgcgg 600
cgtcaacaac cccctcaacc aaaaccccct gcacaggtgc aaatgcgacc aggggagctt 660
ggctccttta gggaaggttt ggctgctctg ccgcgggcga tcgcccaaca attgaaggca 720
ccccttcacc tgcaaacccc tgttgaagca atcaccccag aacccaaggg tggctacctg 780
ctgcgcagtg gtgagcaaac gtggcatgcc cgcagtgtgg tcttggcaac gcctgcctat 840
caaagcgcag agttagtcgc tcccttccag ccagcgatcg cccgtgcttt ggcgaccatc 900
ccctatccca ccgtggcctg tgtggtgttg gcctaccctg ccggactggg acgcagtgta 960
cgccccggat ttggagtact ggttcctcgt ggtcagggca tccgtaccct gggcaccatt 1020
tggtcgtcct gtctttttcc ccaacgcacc cccgccggtt ggcaagtctt caccagtttt 1080
attggcggtg ctacggatcc cgatttggct agccttaggg aagaagcgat tgtcgagcaa 1140
gtgcagcagg atttgacccg cctccttgat ttacccgcag ccaaggcacg attactcggt 1200
atgaaagttt ggcgacgggc gattccccaa tatattgtgg gctaccctca gcagtggcag 1260
cagctgaccc atgccctcac ccaaacccct gggcttttcc tgtgtagtaa ctatgctgag 1320
ggtgtggcct tgggcgatcg cgtcgaacac ggcaatcgaa ccgctgctgc cgtggctgcc 1380
tatcttgcag gaggccagtc ctag 1404
<210> 83
<211> 1431
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 4
<400> 83
atgagtgaac ctattgtcgt agatgttgct gttgttgggg cggggttaag cggcctgagt 60
gttgcatggc ggttgcaaca ggtggcaccg cagtacaagg tggtggtgct ggaggccagc 120
gatcgcctag gcgggaacat cacgaccgaa gcgcgggacg gctttgtgtg ggaattgggc 180
cccaatagtt ttgcgcccac ccctgccctg ctgcacctta ttgccgaggt ggggcttcag 240
gatcaactgc tgcggggcga tcgccgcctg ccccgttaca tttattggcg cggcaagctc 300
caccctttag agcctacccg ccccctcgcc ctcgccacga gcgggttact tagcccttgg 360
ggaaaactgc gagccgcctt aggtgccttt ggctttgtcc cgccctacct tagggatgcc 420
gatgaatccg ttagctcgtt ttttcagcgc cacttaggga aagaggtagc cgagcgattg 480
gttgcgccct ttgtgtctgg ggtttatgct ggcgatccac aacaactgag tgcagcggcg 540
gcctttcggc gcattgtcca actggagcag ctcggcggtg ctctcatacc gggtgcccta 600
cggctacggc ggcagcagcc ccccaagccc acccccccaa agggactcga gatgcgtcca 660
ggggaactgg ggtcgtttca ggagggatta agtgccctcc cgcgggcgat cgcccaccac 720
ttagcagcac ccattcactt acaaacggca ctgcatcagc ttacccccga actcagcggc 780
ggctataccc taagcgccac cacccctgat ggcgagcaag catggcgtgc ccgtagtgtg 840
gtgctggcca cccccgccta tgtcaccgca gacctcctgc ggccatggca acccacaatt 900
gctgccggtc tagaggccat tccctaccca gcggtggcct gcgtggtctt ggcctatcct 960
gccgcggtgg gggtaaccgc tcgtcccggc tttggggtac tgattccccg cactcaagga 1020
ttacgcaccc ttggcacaat ttggtcatct tgcttgtttc ccgagcgcac gccgtctggc 1080
tggcaagtgt ttaccagctt tattggtggg gcaacggatc ctgaattggc gacgcttgaa 1140
cccgaggcca ttgtccagca ggtgcagcag gatttagagc acatgcttgc cttaccccca 1200
gccaaagccc gcttattggg gatgaaactg tggcggcgag ccattcccca atacaccctt 1260
ggctatccgc agcagtggca gcagatcacc cacgccctga agcacctgcc cggcctattc 1320
ctgtgtagta attacgcggc tggcgttgcc ctaggcgatc gcgtcgaaca tgggtataac 1380
actgctgctg cggtggatgc ctacttagga gggacgtatg gctctgtcta a 1431
<210> 84
<211> 1455
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 5
<400> 84
atgacgaacg tagtagatac tttgattgtg ggtgcaggta ttagcggttt gagtctggcc 60
cacgcacttc agaagcaagc taagactgcg cctcccctga agattttagt cgctgagagt 120
cagggacggg tgggcgggaa tatcacaact cagacagatg gagggtttct ctgggaggag 180
ggcccgaata gttttgcgcc gacaccggaa ttgatgaagt tggctgtgga tgtggggctg 240
aagcaggagt tgatttttgc cgatcgcaaa ttgcctcgtt atgtttattg gcaagggaag 300
ctgcaaccgg tgccgatgac tccgcaggcg atgattcagt cccagttact gagttttccg 360
gggaaactgc gggcgcttgt cggggctttg ggctttgtgg ggccggcgat gggttctcaa 420
ttgtcgcagc agggtggaga ggaaactgtt tctcaatttt tccgacgcca tcttggtacg 480
gaagtgatgc agcgattggt ggaacctttt gtttctgggg tttatgcggg cgatccgcaa 540
cagttgagtg cggcggctgc ttttggccgg gtaactcaga tggctgatgt gggtggcggc 600
ctagtggcgg gcgcgctgct ttctgctaga aaaaggccga agaaattgcc cgtagacccg 660
aatattcctc agactaagcc gggggagttg ggttcgttta aacaggggtt gaaggctctg 720
ccagaggcga tcgctgctca attgggcgat cggctcaaac tcaattggca cttgactcgc 780
ctccaacgca cggaacgcca aacttacatt gcggaatttg ctacacctga cggccagcaa 840
caagttgagg cgcgcaccgt cgttttgaca actcccgcct acatcacagc ggagttgttg 900
gcacctctcc aaccggaagt tagcagcgct ttacaagctg ttacttatcc tacggttgcc 960
tgcgttgtct tagcatatcc gctgtcggat gtcaagggta aattagtggg ttttggaaat 1020
ttaattccga ggggccaggg aattcgcacc ctggggacga tttggacatc gagcttattt 1080
cccgatcgcg cccctgctgg ttggcaaact ctcagcaatt acatcggtgg ggcaacagac 1140
tcggatatcg ccaatcttga ccccgaacaa atcgttgggg aggtacatcg agatttatct 1200
cggattttgc tgaagcctct ggcggcgcag cccaaagttt tggctgtgaa cctgtggaag 1260
cgggcgattc ctcagtacaa tttggggcat ctcccgcgcc tgcaacaggt cgagaacggc 1320
ttaaaatcct tgccgggggt gtatttgtgc agcaactacg ttggcggggt ggctttggga 1380
gattgcgtgc ggtggggttt tgagcgggcg atcgaagtca gcgagtattt gcaagaaaca 1440
gggcataggg catag 1455
<210> 85
<211> 1431
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 6
<400> 85
atgacgaaca tagtagatac tttgattgtg ggtgcaggta ttagcggttt gagtctggcc 60
cacgcactcc agaaacaagc taagactgcg cctcccctga agattttagt cgctgagagt 120
cggggacgag tgggcgggaa tatcacaact canngggagg agggcccgaa tagttttatg 180
ccaacaccgg aattgatgaa gttggctgtg gatgtggggc tgaagcagga gttgattttt 240
gccgatcgaa aattgcctcg ttatatttat tggcaaggga aactgcaacc agtgccgatg 300
actccccagg cgatgattca gtcccagttg ctgagttttc cggggaaact gcgggcgctg 360
ttcggggctt tggggtttgt ggggtcggcg atgggttctc aattgtcgca gcagggtgga 420
gaggaaactg tttctcaatt tttccgacgc catcttggta cggaagtgat gcagcgattg 480
gtggaacctt ttgtttctgg ggtttatgcg ggtgatccgc aacagttgag tgcggcggct 540
gcttttggcc gggtaactca gatggctgat gtgggtggcg gcctagtggc gggcgcgctg 600
ctttctgcta gaaaaaggcc gaagaaattg cccgtagacc cgaatattcc tcagactaag 660
ccgggggagt tgggttcgtt taaacagggg ttgaaggctc tgccagaggc gatcgctgct 720
caattaggcg atcggctcaa actcaattgg cacttaactc gcctccaacg cacggaacgc 780
caaacttaca ttgcggaatt tgctacacct gacggccagc aacaagttga ggcgcgcacc 840
gtcgttttga caactcccgc ctacatcaca gcggagttgt tggcacctct ccaaccggaa 900
gttagcagcg ctttacaagc tgttacttat cctacggttg cctgcgttgt attagcatat 960
ccgctgtcgg atgtcaaggg taaattagtg ggttttggaa atttaattcc gaggggccag 1020
ggaattcgca ccctggggac gatttggact tcgagcttat ttcccgatcg cgcccctgct 1080
ggttggcaaa ctctcagcaa ttacatcggt ggggcaacag actcggatat cgccaatctt 1140
gaccccgaac aaatcgttgg ggaggtacat cgagatttat ctcggatgtt gctgaagcct 1200
ctggtggcgc agcccaaagt tttggctgtg aacctgtgga agcgggcgat tcctcagtac 1260
aatttgggtc atattcagcg cctgcaacag gtcgagaacg gcttaaaatc cttgccgggg 1320
gtgtatttgt gcagcaacta cgttggcggt attgctttgg gtgattgcgt gcggtggggt 1380
tttgagcggg cgatcgaagt cagcgagtat ttgcaagaaa cagggcgcta g 1431
<210> 86
<211> 1476
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 7
<400> 86
atggaagttt tagatacctt gattgtgggt gcggggatta gcggtttgag tttggcacac 60
gcacttcaaa aggaagccag gatcgcatcg ccacgggcga ttttggtcgc tgagagtcag 120
ggacgtgtgg gcgggaatat tacgactgcg acaggggagg ggtttctctg ggaggagggc 180
ccgaatagtt tttcgccgac gccggagttg ctgaagttgg ctgtggatgt gggtttgaag 240
caggagttga tttttgccga tcgcaagttg ccgcgttatg tttattggga taagaagctg 300
caaccggtgc cgatgactcc tggggcgatg attcagtctg ggttgctgag ttttccgggg 360
aaactgcggg cgctgttcgg ggctttgggg tttgtggcgc ctgcaatggg ttctcaactt 420
tcgcagcagg gtgatgagga aactgtttct caatttttcc gccgtcatct ggggaaggaa 480
gtgatgcagc gcttggtgga accttttgtg tctggggtgt atgcggggga tccccaacaa 540
cttagcgcgg cggcggcttt tggccgggta acaaagatgg ctgatgcggg gggctctctg 600
gtggcggggg cgctgctttc tgctaggaaa aaaaaacccc aaccccccct tggtaaaggg 660
gggctaaatg ctcttgcaga cccgaatatt ccgaagacta agcgggggga gttgggttcg 720
tttaaagggg ggttgaaggc tctgccagag gcgatcgctg cttctttggg cgatcgagtg 780
aaactcaact ggcatttgac taggctcgat cgcactgaac gggaaactta cattgctgta 840
ttttcaacac ccgacggaca gcaggaaatt gaggcgcgca cggtggtttt gacgaccccg 900
gcttacgtga cagcggagtt gttgcagcct ctgcaaccgt ccgttagcag cgctttacaa 960
gcttttactt atcctacggt tgcatcggtt gtattagcat atccgatgtc ggatgtcaag 1020
ggcaaattag tgggatttgg aaatttaatt ccgagagggc agggaattcg cactctgggg 1080
acgatttgga catcgagttt atttcccgat cgcgcgcctg cgggctggca aactctcacc 1140
agttatattg gcggagcaac tgattcggga attggcaatc ttgacgccga acaaattgtt 1200
ggggaggtgc accgagattt gtctaggatt ttgctgaagc cggaggcggc gcagccgaaa 1260
gttttgactg tgaaactttg gaagcgggcg attcctcagt acaatttggg ttatttcgat 1320
cgcctgcaac agatcgatcg gggtttaaaa tctttgcccg ggctgtattt gtgcagcaac 1380
tacttaggcg gggtggcttt gggagattgc gtgcgcaggg gctttgagcg agcgcaagaa 1440
gtgggcgagt atttgaacga tagttttgag ttttga 1476
<210> 87
<211> 1404
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 8
<400> 87
atggacgatc gccaagggga gccaaccgag gttttggtgg tgggggccgg catttgcggc 60
ttgacggtgg cccatggctt ggcgcagcgg ggtgtggcct gtcgggtggt ggaagccagt 120
gaccgcgtgg ggggcgcgat cgtcacccgg cgggagcatg gctttcagtg ggaggagggc 180
cccaacagct tttcgcccac cccggagttg ttggctttgg cgatcgccat gggtttgcgg 240
gacgagttga ttttggctga ccggcggttg ccccggtatg tgtggtggca aaaccgtttg 300
caggcggtac ccatggcccc accgggtctc ctgacgacgg gtttgttgtc gccgtggggc 360
aaattgcggg cggcggtggg ggccttgggg tttgtgcccc ccagctttgc ggcggatgag 420
acggtgggga gttttttccg gcggcatttg gggccggagg ttttgacgcg gttggcggca 480
ccgtttgtat cgggggtgta tgccggcgat cccgaagccc tctccattgg cgcggctttt 540
ccccgggtga cggcgatcga agcgatgggg ggcggcctgg tggcggggtt tatccaggcc 600
ctgcggcagc ggggggcccc cgatccccat ttgccccaga ccaagccggg ggagttgggt 660
tccttccggg aagggatcga ggctttgccc cgggcgatcg ccgccgattt agcccaacgg 720
ggggtgaccc taaccttgaa ccaagcctta acccgattgg aacccacggg cgatcgctgg 780
cgggcggtgt tggccagcgg cgaggaattg gaagcccgga gtgtggtgtt ggcggtgccg 840
gcgtgggcgg cggcgaaaat tctccagcaa accccagaaa ccgccgattg ggtggcggat 900
ttggagagaa ttccctatcc ggcggtggcc tgtgcggtgt tggcctatcc cgacggggat 960
ttgcggcagc cgttgcgggg atttgggcac ttggtgccgc gggggcaagg cattcgcacc 1020
ctgggcacca tttgggcatc cagtttgttt ccggggcggg cccccgccgg atacaccctc 1080
ctgctgaatt tcattggcgg caccaccgat cccacactgg cgcagttgga cgcaggggcg 1140
atcgcccaag cggtccacca ggacttgcga caaattttgg ttcaaccgac ggcggcgccc 1200
cccaaagtat tggcggtgaa cctgtggcgg cgggcgatcc cgcagtttac ggtggggcac 1260
caagggcgct tggcgcggct ggcggcgggt ttaccaccgg ggttggtttt ggccgggaat 1320
tatcgggggg gggtggccct cggcgattgc gtgcggcacg ggctggcggt ggcggcccaa 1380
gttgcggctt ttcttgagag ataa 1404
<210> 88
<211> 1410
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 9
<400> 88
atgctggata cgctcgtagt tggggcaggt ttgagcggat tgagcgtcgc gcgatcgctc 60
caaatggcag ggcgcaatgt gctggttgcc gaggcgcagg agcgtgttgg cggtaacatt 120
ctcacgcagc agagcgatga tgggtttcag tgggaggagg ggccaaatag cttctcgcca 180
aaccgcgaac tgctcgaact cgcagtcggt gtggggctca gggacaagct gatttttgca 240
gatcgccgtt tgccgcgttt tgtttactgg cgcaatagtt tgcatccggt cccaatgagc 300
ccgccgcgag cagtgacgac atcgctattg agtcccttag gtaaattacg cgcggtagcc 360
ggcgcgatcg gatttgtgcc cccagcgctc tccgacgagg agtccgttgc cgagttcttc 420
acgcgccatt tggggtccga agttgccgaa cgactggttg cgcctttcgt ttcgggggtt 480
tacgcaggtg atgtcgagcg attgagtgtc agctcagcct tccggcaagt cgctaagctg 540
tccgaggtcg gtggcgggtt gcttgctgga gcactcctga cgcgccgtgg taaatccggc 600
cctcctcaaa agctccctcc aagggttgac cccaatttgc cgcgcgtcca acgcggtgag 660
ttggggtctt tcgtcggcgg gttaaaaatg ctgcccgagg cgatcgccgc taaactcggc 720
accaagctta agctgcactg gaccctagaa cacttagttc gctgcgaggg cggctatcgc 780
gccgaattcg caactccgga ggggcaacag cagatcgagg tgcgttcgct ggtattggca 840
acacctgctt acagatgcgc gcgggtattg caatcgctcg ctccttcggc ttgccaagcg 900
ctatccgaaa tgccttatcc agcagtagcg tgcgtagtgc tggcgtatcc gagctcggcg 960
ttttcctctc ctttacaagg gtttggcaat ttgattcccc gcggacaagg tattcgcacg 1020
ctgggtacaa tttggtcgtc ggcacttttc cctgggcgaa cgccgccagg ctgggagatc 1080
ttaacgagtt ttattggcgg cgcgaccgac cccgaactcg gtacgctgtc cgcacagcaa 1140
atcgtggcag aggtccgtcg cgacctgcag cgggtgttgg tcgataaaga acccaacacc 1200
gaaccgcgcg tcctggcagc taaggtttgg ccgcgcgcga tccctcagta cgtgctcggt 1260
catagcgatc gcctcaaccg catccaaatc gacctcgacc ggctgcccgg cttatatttg 1320
tgcagcaact tcaccgatgg cgtagcgctc ggcgactgcg tgcggcgggg cttcgagact 1380
gctgaggcga tcggacagta cctcaactga 1410
<210> 89
<211> 1416
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 10
<400> 89
atgctaaagt tggcggtaga tgttggcctt aaacaggatt taatttttgc agatcgtaag 60
ctacctcgct atgtttattg gaatggtcaa ttacntttag ttgctgagag ccaaaatcga 120
gtagggggta atattacaac tgtttctcaa ggagatttta tttgggaaga aggtcctaat 180
agtttttctc ctacacctga actgctaaag ttggcggtag atgttggcct taaacaggat 240
ttaatttttg cagatcgtaa gctacctcgc tatgtttatt ggaatggtca attactacca 300
gtgccaatgg gtccaacggc gatgttacag tctaagctac tgagtgattc tggaaagtta 360
cgcgctttag ttggtgcttt gggttttgtg ccacctgctg tgggtactgg tctgtctcag 420
caagggggag aagaaactgt atctcaattt ttccaaagac atctgggtgt ggaggtgatg 480
cagcgattgg tagaaccttt tgtttctggc gtttatgctg gagaccctag tcaacttagt 540
gctactgctg ctttttctag agtagcgaga atggccgata ttggtggtgg acttttggct 600
ggtgctgttt tgtctgctaa aagaaatcct aagtcaaagg ttgctgccga tcctaatatt 660
cccaagacta aacctggaga gctaggttct ttccggggtg gtttggaggc attgccaaag 720
gcgatcgcta cttatttagg agaagcggta aaactaaatt ggcatctcat tggtattcgt 780
cgtacagaac aacaaactta tatagcagaa ttttctactc ccaacggttc tgagcaaata 840
gaaactcgta ctattgctct atctactcct gcttattttt gttctgaatt atttaaacct 900
ttattgcctg aaattgcttc tactttcgat gaattttatt atcctactgt tgcttgtgta 960
gttttggcat atcctgtttc ttctattaag gcaaagatag atggctttgg taatttaatt 1020
cctagaggtc aaggtatcag gactcttggt actatttggt cttctgcttt gtttcctggt 1080
agaacacctc caggatggca agtttttact aattttattg ggggtgcaac agacccagga 1140
atttctcagt tagatagtga ggcgatcgtt tcacgagtac atcaagacct tgggcaaact 1200
ttattaaagc aagatgcgga acaaccaaaa gttctggctg tacatttatg gtctcgtgct 1260
attcctcagt ataatttagg tcataattct aggctggatc agattaatca tggtttaaaa 1320
tcttggcctg gtgtgtatct ttgtagtaat tatattggtg gtgtagcttt gggagattgt 1380
gttcngtaca tcaagacctt gggcaaactt tattaa 1416
<210> 90
<211> 1464
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 11
<400> 90
atggaagttt tagatacctt gattgtgggt gcggggatta gcggtttgag tttggcgcac 60
gcacttcaaa aagaagcaac gagtgcatcg ccacgggcga ttttggtggc tgagagtcag 120
ggacgtgtgg gcgggaatat cacgactgcg acaggggagg ggtttctctg ggaggagggc 180
ccgaatagtt tttcgccgac gccggagttg ctgaagttgg ctgtggatgt cggtttgaag 240
caggagttga tttttgccga tcgcaaattg cctcgttatg tttattggga aaagaagctg 300
caaccggtgc cgatgactcc tggggcgatg attcagtctc agttgctgag ttttccgggg 360
aaactgcggg cgctgttcgg ggctttgggg tttgtggggc cggcaatggg ttctcaactt 420
tcgcagcagg gtgatgagga aactgtttct caatttttcc gccgtcatct gggaaaggaa 480
gtgatgcagc gcttggtgga accttttgtg tctggggttt atgcggggga tccccaacaa 540
cttagcgcgg cggctgcttt tggccgggta acaaagatgg ctgatgcggg tggcgggctg 600
gtggcggggg cgctgctttc tgctaggaaa agaccccccc aaccccccct taccaagggg 660
gggctaaaga ctgttgcaga cccgaatatt ccgaagacta agccggggga gttgggttcg 720
tttaaagggg ggttgcaggc tctgccagag gcgatcgctg cgaacttggg cgatcgactg 780
aaactcaact ggcacctgac tcgactcgat cgcacggaac gcgacactta catagctgta 840
ttttctacgc ccgacggaca gcaggaagtt gaggcccgaa ccgtagtttt gacaacaccg 900
gcctatgtta cagccgagtt gttggagcct ctgcaaccgt ccgttagcag cgctttacaa 960
gcttttactt atcctacggt tgcctccgtt gtattggcat atccgatgtc ggatctcaag 1020
ggtaaattag tgggatttgg aaatttaatt ccgagggggc agggaattcg cactctgggg 1080
acgatttgga catcgagttt atttgccgat cgcgcgcctg cgggctggca aactctcacc 1140
agttatatcg gcggggctac tgattcggga attggcaatc ttgacgccga acaaattgtt 1200
ggggaggtgc accgagattt gtctcagatt ttgctgaagc cggaggcggc gcagccgaaa 1260
gtgttgactg tgaaaatttg gaagcgggcg attcctcagt acaatttggg tcatttcgat 1320
cgcctgcaac agatcgatcg gggcttaaaa tctttgccgg ggctgtattt gtgcaccaac 1380
tactttggcg gtgtggcttt gggagattgc gtgcggagag gttttgagcg ggctcaagaa 1440
gtggacgagt atttgaacgg atag 1464
<210> 91
<211> 1428
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 12
<400> 91
atggtagata ctctaatcat tggtgccgga ataagcggtc ttagcctagc ctacgcctta 60
catcaagacg ggcgaaaagt tttgctgtgc gaacgtcaag aacgagttgg cggaaatatt 120
acaacaggta aggcgggtgg gtttctttgg gaagaagggc caaccagctt tacaccaaca 180
ccagcactgc taaagctggc agtagatgtg gggttgagag aggagttggt gttagcagac 240
caccgcttac ctcgttttgt ctactggaaa ggtcagctac ttcctgtacc gatgagtcca 300
ccatcggctg tcacatccaa gttactaagc ttgtcaggca agtttagggc tttagtaggt 360
gctttagggt ttatcccacc agcaataggc aaccacttat ctcagcaagg aggcgaggaa 420
acagttgctc agttttttaa gcgccattta gggacagaag tggcagaaag attggtagcg 480
ccgtttgtct ctggtgtcta tgctggtgat gtgcatcaac tcagcgcccg ttcagctttt 540
cggcggatcg ctcaactaga aaatgtgggc gggggactgg tatctggggc aatattgtca 600
cggaagcaac gccagcaaca gaaaccgcca acagatccca gtttgcctac tgttcgccgt 660
ggtgaattag gctcgtttaa agagggttta caatctctac caaaagcgat cgcatcccat 720
ctgggtgaaa atattaaact aaattggact ttgactgagc ttcgtcaaac agccaatcaa 780
acttatattg cagaattttc taccccagaa ggtagtcagc aggtagaagc gcgtactgtt 840
gttctaacta ccccagctta tgtaaccgcc gaattgctgc ataatctagc accaaatgcg 900
agtattgcct taaaagaaat tccttatccc tcagtagctt gcgttgtctt agcttatcca 960
gacgatgcct taaaatttcc cctaaaaggc tttggaaact taattccacg aggtcaaggt 1020
attcggactc taggcacaat ttggtcttct agcttatttc caggacgtgc gccgcaaggt 1080
tggcaaatgc taactaattt tattggtggg gctacagatc cagaagtagg taatctagac 1140
aacgagcaat tagttcaagc agtccataaa gacttgcagc gcgttcttct taaaaaagat 1200
gtacctccaa aagcgatcgc agtacattta tggaaacggg caattcctca atatacttta 1260
ggtcatcatc tgcgtttagc ccaaatcaat caagatttgg cacaactacc tggtttatat 1320
ctatgtagta actacactga tggcgtttct ttaggtgatt gtgtgcaacg cgcctacgat 1380
caattaccaa ttatcaataa acaactatca attatcaatg acaattag 1428
<210> 92
<211> 1464
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 13
<400> 92
atggaagttt tagatacctt gattgtgggt gcggggatta gcggtttgag tttggcgcac 60
gcacttcaaa aagaagcaac gagtgcatcg ccacgggcga ttttggtggc tgagagtcag 120
ggacgtgtgg gcgggaatat cacgactgcg acaggggagg ggtttctctg ggaggagggc 180
ccgaatagtt tttcgccgac gccggagttg ctgaagttgg ctgtggatgt cggtttgaag 240
caggagttga tttttgccga tcgcaaattg cctcgttttg tttattggga aaagaagctg 300
caaccggtgc cgatgactcc tggggcgatg attcagtctc agttgctgag ttttccgggg 360
aaactgcggg cgctgttcgg ggctttgggg tttgtggggc cggcaatggg ttctcaactt 420
tcgcagcagg gtgatgagga aactgtttct caatttttcc gccgtcatct gggaaaggaa 480
gtgatgcagc gcttggtgga accttttgtg tctggggttt atgcggggga tccccaacaa 540
cttagcgcgg cggctgcttt tggccgggta acaaagatgg ctgatgcggg tggcgggctg 600
gtggcggggg cgctgctttc tgctaggaaa agaccccccc aaccccccct taccaagggg 660
gggctaaaga ctgttgcaga cccgaatatt ccgaagacta agccggggga gttgggttcg 720
tttaaagggg ggttgcaggc tctgccagag gcgatcgctg cgaacttggg cgatcgactg 780
aaactcaact ggcacctgac tcgactcgat cgcacggaac gcgacactta catagctgta 840
ttttctacgc ccgacggaca gcaggaagtt gaggcccgaa ccgtagtttt gacaacaccg 900
gcctatgtta cagccgagtt gttggagcct ctgcaaccgt ccgttagcag cgctttacaa 960
gcttttactt atcctacggt tgcctccgtt gtattggcat atccgatgtc ggatctcaag 1020
ggtaaattag tgggatttgg aaatttaatt ccgagggggc agggaattcg cactctgggg 1080
acgatttgga catcgagttt atttgccgat cgcgcgcctg cgggctggca aactctcacc 1140
agttatatcg gcggggctac tgattcggga attggcaatc ttgacgccga acaaattgtt 1200
ggggaggtgc accgagattt gtctcagatt ttgctgaagc cggaggcggc gcagccgaaa 1260
gtgttgactg tgaaaatttg gaagcgggcg attcctcagt acaatttggg tcatttcgat 1320
cgcctgcaac agatcgatcg gggcttaaaa tctttgccgg ggctgtattt gtgcaccaac 1380
tactttggcg gtgtggcttt gggagattgc gtgcggagag gttttgagcg ggctcaagaa 1440
gtggacgagt atttgaacgg atag 1464
<210> 93
<211> 1464
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 14
<400> 93
atggaagttt tagatacctt gattgtgggt gcggggatta gcggtttgag tttggcgcac 60
gcacttcaaa aggaagcgag gagtgcatcg cctcgggcga ttttggtggc tgagagtcag 120
ggacgtgtgg gcggaaatat cacgactgcg acaggggagg ggtttctctg ggaggagggc 180
ccgaatagtt tttcgccgac gccggagttg ctgaagttgg ctgtggatgt cggtttgaag 240
caggagttga tttttgccga tcgcaaattg cctcgttttg tttattggga aaagaagctg 300
caaccggtgc cgatgactcc tggggcgatg attcagtctc agttgctgag ttttccgggg 360
aaactgcggg cgctgttcgg ggctttgggg tttgtggggc cggcaatggg ttctcaactt 420
tcgcagcagg gtgatgagga aactgtttct caatttttcc gccgtcatct gggaaaggaa 480
gtgatgcagc gcttggtgga accttttgtg tctggggttt atgcggggga tccccaacaa 540
cttagcgcgg cggctgcttt tggccgggta acaaagatgg ctgatgcggg tggcgggctg 600
gtggcggggg cgctgctttc tgctaggaaa agaccccccc aaccccccct taccaagggg 660
gggctaaaga ctgttgcaga cccgaatatt ccgaagacta agccggggga gttgggctcg 720
tttaaagggg ggttgaaggc tctgccagag gcgatcgctg cgaacttggg cgatcgactg 780
aaactcaact ggcacctgac tcgactcgat cgcacggaac gcgacactta catagctgta 840
ttttctacgc ccgacggaca gcaggaagtt gaggcccgaa ccgtagtttt gacaacaccg 900
gcctatgtta cagcggagtt gttggagcct ctgcaacctt ccgttagcag cgctttacaa 960
gcttttactt atcctacggt tgcctccgtt gtattggcat atccgatgtc ggatctcaag 1020
ggtaaattag tgggatttgg aaatttaatt ccgagggggc agggaattcg cactctgggg 1080
acgatttgga catcgagttt atttgccgat cgcgcccctg cgggctggca aactctcacc 1140
agttatatcg gcggggctac tgattcggga attggcaatc ttgacgccga acaaattgtt 1200
ggggaggtgc accgagattt gtctcagatt ttgctgaagc cggaggcggc gcagccgaaa 1260
gtgttgactg tgaaaatttg gaagcgggcg attcctcagt acaatttggg tcatttcgat 1320
cgcctggaac agatcaatcg gggcttaaaa tctttgccgg ggctgtattt gtgcaccaac 1380
tactttggcg gtgtggcttt gggagattgc gtgcggagag gttttgagcg ggctcaagaa 1440
gtggacgagt atttgaacgg atag 1464
<210> 94
<211> 1437
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 15
<400> 94
atgctggatg cgttagtcgt gggcgctggc atcagcggtt tgagcttggg gcgcaccctg 60
cagcagcagc agcactcgct gctcgtggcc gagcgccaac cccaggttgg gggcaacatt 120
accaccagca gcactggcga gtttttgtgg gaggaagggc ccaacagctt ctcgccggcg 180
cgagagctgc ttgagcttgc cgtcgatgct ggcctgcgcg aggagctcgt gctggccgac 240
cgacagctgc cgcgctacat ctactggcgg gggcgactgc agccggtgcc catgcgccct 300
tcggccgcgc tcaaaacgca gctgctgagc cctagcggca agctgcgagc gctggccggg 360
gcgctgggat ttgtcccccc ggcggtgggc gaggcggtat cgcagcaagg cggcgaggaa 420
acggtcgcgc agtttttcca acgccatctg gggcgcgagg ttaccgagcg tttggtcgcc 480
ccgttcgtct cgggcgttta cgccggcgac gtccgccagt tgagcgccca ggccgccttc 540
cggcgcgtca cgcagctcgc cgatcgcggc ggcggcctgc tccccggagc gcttctgggg 600
cggcaggcgg ccccgcaacg cccggctacc agccagacgc agctccccca aacccaaagt 660
ggggagctgg gctcgttccg cacgggcttg caggcgcttc ccaacgccat tgccgagcgc 720
ttgggggatg ccctgcgctg caactgggcg ctatcgcagc tgcagcgcac cgagcgcgac 780
agctatttgg ccaccttcca aacgccccag gggccgcagc aggttgaggc gcgtgcggtg 840
gtgctcacca ccccagccta taccagcgcc gagctattgc gcccgctgca cgcccgcgcc 900
agccagcggc tggctgccat tccctacccg ccggtggcta gcgtggtgct ggcctacccg 960
cagcaggcgt tgggcggggc cctgcgcggc tttggcaacc tcaacccgcg cggtcaaggc 1020
attcgcaccc tgggcacgat ttggtcgtcg gcgctgtttg ccggccgcgc gccggctggt 1080
tgggaaatcc tgactagctt tattgggggc gccactgacc ctgaaatcgc tcgcctggat 1140
gaagatcgca tcgtgcaagc cgtccacgcc gatttgcgcc aggtcctgct ggcgcgctac 1200
gtggcaccca aggtgctggc cgtgcggctc tggcaccgcg ccattccgca gtacgccatc 1260
ggccaccagg cccaaatgca agccctcgaa gccgagctag ccgagctgcc ggggctgttt 1320
ctgtgcagca actacctcga tggggtctcg ctgggcgatt gcatccgccg cggcttcgag 1380
cgggcgcaca cggtttcgca ctacttgcaa gcgcctgcgg cagctccagc ggcctga 1437
<210> 95
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 16
<400> 95
atgactcacg tactcgatag tttgatcgtc ggtgctggca ttagcggcct gagtttagct 60
cattctctcc ttcggaaccc aaaccctcaa ttgtctctca acattctggt gagcgagcat 120
caaggacggg taggaggaaa cataaccaca gtatctcaag gagagtttct ctgggaagaa 180
ggccccaata gtttctctcc gacccctgag ttactgaagt tagccgtaga agttggcctc 240
aagtctgagt tcgtctttgc cgatcgcaag ttacctcggt atgtttactg gaatagtcaa 300
cttatgccag tgccgatgag tcctccggct ttgttgagta caaaactctt aagtcctgga 360
ggtaaactcc gagcattaac gggggcatta ggatttgtac ggccggcgat gggacaagcg 420
ttaagtcaac aaaatgggga agaaacgatc tcgcagtttt ttgagcgtca tttgggttca 480
gaagttctca aacgactggt tgaacccttt gtttccgggg tctatgcagg cgatccacag 540
caactcgaaa ttagctcggc ttttgcccga gtcgcacgta tggcttacag tggcggtggg 600
ttagttgccg gagcggtttt atcacgtggt cagaacaaat cttcgcgatc gcctgccgat 660
ccgtctattc cccaaactaa acggggagag ttggggtctt ttcgtcaggg aattggagcc 720
ttacccaatg cgatcgcgca gcagttaggc gatcaactca aattaaactg gcaactcact 780
cgtcttgaac ggactgaaaa ccaaacttat cgggctgaat tttcgacccc agatggcgtt 840
caacaggtcg aaactcgaac ggtagtgttg acaactccgg cttatgtcac agcagaaatt 900
ctgaaaccgt tgcaactcca agtcagtcaa acgttaaccg aaattcctta tcctccggtg 960
gcttgcgtcg ttttagccta tcccgtttca gctttcaagc agaaattaac cggatttggg 1020
aatttagttc cccgaggaca aggaattcgg acgttaggca cgatttggac ctcaagttta 1080
tttcccggtc gcgcccccca aggctggcaa gttctcacca gttatattgg cggagcgact 1140
gatccagaaa ttggagagtt agaagatgat caaattgttg aggcggttca tcaagatttg 1200
cgtcgcattt tactcaaaga agatatctct cccaaagtgc tagccgtgca tctgtggaaa 1260
cgtgctatcc ctcaatacaa tctcggacac caacaacggt tacaacaggt taatgaaggt 1320
ctagaggcaa tgccagggtt atatctgtgt agcaactata tcgacggtgt agcgttagga 1380
gattgtgtgc gtcgttctat aggacaagct aacgaaattc tcagtttttt ggatcaatag 1440
1440
<210> 96
<211> 1446
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 17
<400> 96
atgacgaatg tagtagatag cttgattgtg ggtgctggta ttactggctt gagtctggcc 60
cacgcacttc ataaggaagc aaagactgga actccggtga agattttggt tgctgagagt 120
ctgggacgtg tgggggggaa tatcacaact tgtacaggga atgggtttct gtgggaggag 180
ggcccgaata gtttttcgcc gacggtggag ttgatgaagt tggctgtgga tgtggggctg 240
aaggaggagt tgatttttgc cgatcgcaaa ttacctcgtt ttgtatattg gcaaaataag 300
ctgcaaccgg tgccgatgac tccacaggcg atgattcagt cgcaattgtt gagttttccg 360
gggaaactgc gggcgctgtt cggggctttg ggatttgtgg caccggcaat gggtgctaca 420
ctttcgcagc agggtggtca ggaaactatt tctcaatttt tccggcgaca tctgggtacg 480
gaagtgatgc agcgattggt ggaaccgttt gtttctggag tttatgccgg cgatcccgaa 540
caacttagcg cggcggcggc ttttggtcgg gtaacgagga tggctgattt gggtggtgga 600
ctggtggcgg gggcgctgct tggggcgagg aaagggccga agaaaatgcc cgcagacccg 660
aatattccca agactaagcc gggggagttg ggttcgttta aaggggggtt gaaggctcta 720
ccagaggcga tcgctgctca attaggcgat cgcctgaaac tcaattggca tctcacaggt 780
ttgcaccgta ctgaaaacaa aacttacatt gctgaattct caacgccgga tgggccgcaa 840
caggttgaga ctcgcactgt ggtgttgaca actcctgctt atgtgacagc cgattggttc 900
caatctctgc aaccggaagt gagcacggcc ttacaagctt ttacttaccc tactgttgcc 960
tgcgttgtcc tagcatatcc gaaatcggat gtcaaggaaa agatggtggg ctttggaaat 1020
ttaattccga ggggacaggg aattcggact ctggggacga tttggagttc gagtttattt 1080
gctaatcgtg cgcctgcggg ttggcaaact ttgacgagtt ttatcggcgg ggctactgat 1140
tcgggtattg cgaatcttga cgctgaacaa attgtggccc aggtgcatcg agatttgtct 1200
cggattttgc tgaaaccgga tgtcccacag ccgaaggttt tggcggtgaa ggtgtggaag 1260
caggcgattc ctcagtacaa tttggggcat ttcgatcgcc tggaacagat cgatcgaggt 1320
ttaaaatctt tgccgggggt ttatttgtgt agcaattacc ttggtggggt ggctttggga 1380
gattgtgtgc gtctgggttt ggaaagggcg atcgaggtta gtaagtattt gcaagaaacc 1440
agctag 1446
<210> 97
<211> 1446
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 18
<400> 97
atgacgaacg tagtagatac tttgattgtg ggtgctggta ttactggctt gagtctggcc 60
cacgcacttc agaaggaagc aaagactgga actccggtga agattttggt tgctgagagt 120
ctgggacgtg tgggggggaa tatcacaact tgtacaggga atgggtttct gtgggaggag 180
ggcccgaata gtttttcacc gacggcggag ttgatgaagt tggctgtgga tgtggggctg 240
aaggaggagt tgatttttgc cgatcgcaaa ttgcctcgtt ttgtatattg gcaaaataag 300
ctgcaaccgg tgccgatgac tccgcaggcg atgattcagt cgcaattgtt gagttttccg 360
gggaaactgc gggcgctgtt cggggctttg ggatttgtgg caccggcaat gggtgctaca 420
ctttcgcagc agggtggtca ggagactgtt tctcaatttt tcgggcgaca tctcggtacg 480
gaagtgatgc agcgattggt ggaaccgttt gtttctgggg tttatgcggg cgatccccaa 540
caacttagcg cggcggcggc ttttggtcgg gtgacgagga tggctgattt gggtggtgga 600
ctggtggcgg gggcgctgct tggggcgagg aaagcgccga agaaaatgcc cgcagacccg 660
aatgttccga agactaagcc gggggagttg ggttcgttta agggggggtt gaaggctctg 720
ccagaggcga tcgctgctca attaggcgat cgcctgaaac tcaattggca tctcacaggt 780
ttgcaccgta ctgaaaacaa aacttatatt gctgaatttt cgacgccgga tgggccgcaa 840
caggttgaga ctcgcactgt ggtgttgaca actcctgctt atgtgacagc ggatttgttc 900
caatctctgc aaccggaagt tagcagcgcc ttacaagctt ttacttaccc tactgttgcc 960
tgcgttgtct tagcatatcc gaaatcggat gtcaaggaaa agatggtggg atttggaaat 1020
ttaattccga gggggcaggg aattcggact ctggggacga tttggacttc gagtttattt 1080
gctaatcgcg cgcctgcggg ttggcagact ttgacgagtt atatcggtgg ggctactgat 1140
tcggggattg ggaatcttga tgctgaacaa attgttgggg aggtgcatcg agatttgtct 1200
cggattttgc tgaaacctaa tgtgcctcag ccgaaggttt tggcggtgaa ggtgtggaag 1260
caggcgattc ctcagtacaa tttggggcat ttcgatcgcc tggaacggat cgatcgaggt 1320
ttaaaatctt tgccgggggt ttatttgtgt agcaactacg ttggtggggt ggctttggga 1380
gattgtgtgc gtctgggttt tgaaagggcg atcgaggtta gtgagtattt gcaagcaacc 1440
agctag 1446
<210> 98
<211> 1401
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 19
<400> 98
atgttagata ctttaattgt aggagcagga atcagcggtt taagcacggc ctatcgcctg 60
catcaagggc aacgccagat tttagtcgca gaacagcgcg atcgcgttgg cggcaatatt 120
gcaacggaac accaggggga attcctctgg gaagaaggcc ccaatagttt ttccccaacg 180
ccggagttat taaaactggc agttgatgcg ggcttaaaaa atgactttgt tttcgctgat 240
cgcaatcttc cccgttacgt ttattggcag ggaaaactgc gtcctgttcc catgagtccc 300
ccagcagcag ttaaatcgca attgcttagc ccttggggaa aactgcgggc attagccggt 360
gccctgggct ttgtttcccc taatgttgaa gggaaggaag agacagttgc cgactttttt 420
acccgtcact tgggagaaga agtggcgcaa cgcctcgtcg ctccctttgt gtctggcgtt 480
tacgccggag atgttcaccg gctcagtgcc caagccgcct ttggccgggt tacgcaattg 540
gctgatgtgg gcggtggact ggttgcaggg gcagtattat cccgcggtaa gagaaagcag 600
tcttcatcga cggtaactga gaatgccgat attcccaaaa ccaagtccgg ggaattggga 660
tcctttcgag aaggcttgca aatgttgccg cgcgcgatcg cgtcaaaact gggcgaatca 720
gttaaattaa actggcagct taacaatatt tctcctcatc ccgaacaagg ctacattgcc 780
gcattttcca ccccggaagg ggaacaaagc gtagaagcta aaagtattgt cctcactaca 840
ccagctcatg tcactgcccc aattattcaa accttatccc cgctaaccag cacagcactc 900
caagatattt cttatccgcc agtagcctgt gttattcttg cctatccaga tgaggcgctg 960
cgcttctcct taaaaggatt tggcaacctg gttccccgca accaaggctt gcgtacgcta 1020
ggcacaattt gggcatcaac gctatttcct gggcgtgccc ctcaaggttg gcacattctg 1080
acaaacttta tcggtggggc aacagatccc gaaattgctc aacttagtga agaacaaatc 1140
atcgaccaag ttcaccagga cttacaaaag gtgttactga aatcggatac gaaccccaaa 1200
cccttagcgg ttcatctgtg gtcaaaagcc attccgcaat atactttagg acatctcgac 1260
cgtttagaaa cgataagaaa tagtctaaaa tcttgtcctg gcctcttttt atgcagcaat 1320
tatcttgatg gggtttcact gggagattgt gtccgtcgcg gcgaagaaac cgctcaagcc 1380
gtgttagatt atttgggtta a 1401
<210> 99
<211> 1449
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 20
<400> 99
atgactgaaa tattagatgt cttagtcgtg ggtgcaggta tcagtggctt gagtttagcc 60
cataaactga caaaattaag taataattca cccttgaaaa ttttagttgc tgagagccaa 120
aaccgagtcg ggggtaatat cacgacagtt tctcaaggag aatttctttg ggaagaaggt 180
cctaatagtt tttctcccac ccctgagctt ttaaagttgg ctgtagatgt tggcctcaaa 240
gaggatttga tttttgcaga ccgtaagtta cctcgttatg tttattggaa tggtcaatta 300
ctaccagtac caatgagccc aaaggcaatg ttacagtctc aactactgag taatactgga 360
aagttacgcg ctttagttgg tgctttgggt tttgttccac ctgttgttgg tatggatcta 420
tctcaggaag ggggagaaga aactgtatcc caatttttcc aaagacatct tggtaaagag 480
gtaatggaac gattagttga accttttgtt tctggtgttt acgctggaga ccctagtcaa 540
cttagtgcta ctgctgcttt ttctaaagtg gctcgaatgg ctgaccttgg tggtggactt 600
ttggctggtg ctgttttgtc tgctcaaaga aaccctaagt caaaggctgc tgctaattct 660
aatattccta aaactaaacc tggagagttg ggttctttcc gacgtggttt ggaggtattg 720
ccaaaagcga tcgctactta tttaggacaa gcagtcaagc tcaattggtc tctcgtgggt 780
cttcgtccga cagaaaaaca gacttatata gcagaatttt cgactcccaa tggttctcag 840
caaatagaaa ctcgtaccat tgccctctcc agccctgctt atgcctgtgc taaattattt 900
agacctttac ttcctgaaat tgctggtact cttgatgaat tctattatcc tactgttgcc 960
tgtgtagttt tggcatatcc tgtttcttct atcaaggcca agatagatgg ctttggtaat 1020
ttgattccta gaggtcaagg cattcggact cttggtacta tttggtcttc tgctttattt 1080
tctggtagaa cacctcttgg atggcaaatt tttactaatt atattggtgg tgcaacagac 1140
ccagaaattt ctcacttaga tagtgaagcc atcgttgcac aagttcatca agacctttgt 1200
caaactctat taaaccagaa tcctgaaaaa ccaaaagttc tggcagtaca tatatggtct 1260
cgtgctattc ctcagtataa tttaggttat agttctaggc tggctcagat taatcatggc 1320
ttaaaatctt ggcctggagt atatctatgt agtaattata ttggtggtgt ggctctggga 1380
gattgtgttc gtcgtagtat agaagtagcg actgatattt attcgggtat ggggtttggg 1440
gagctttaa 1449
<210> 100
<211> 1497
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 21
<400> 100
atgaatgcca ttgcggaaaa tggaaacgag gcaaacgaag catcgcctgt atgggtagat 60
accttaattg tgggtggtgg tataagcgga ttgagcgtgg ctcatgcttt ggtcaactgc 120
gatcgctcgg tggacaatat tttactggcg gaaagccaac ctcgggtggg aggcagcgta 180
accagtgctt cgagtgaggg atttttgtac gaagaaggtc ccaatagttt ttcgcccaca 240
cccgaactgc tgcaacttgc tgtggatgtc ggtttgaaag atgagttgat tttggctgac 300
cgccgcttgc cgcgctacgt ttactggcaa ggaagattga ttgcactgcc gaacagtccg 360
cccagtgccg ttacttcccc tattttaagt cctgtgggca aactacgggc gctgttgggg 420
gcattgggat ttgtaccgcc ccacgtgacc agcgaaccgg aaagcgtttc cgattttatc 480
cgccgccacc tgggacctga agtcttgcaa aaattggtgg aaccgtttac ttctggggta 540
tatgctggta accccgacga actggaagcg gcttctgctt ttgcccggat agcacgtttg 600
gaaaaagttg ggggcagctt ggtagcggga gctattttat cgcggcgtca ggcaccaacg 660
cagaaaaaaa accgcgatcg caatttgcca aaaacgcaac gcgggcagtt aggttccttt 720
caacggggct tgcagtcttt gccggaagcc attgccggaa aattgggttc gcgagtgcgg 780
gtgaactggc aggcaaaaag catcggtaaa acggaaggtg gcaattattt ggtggaattt 840
gctaccccgc aggggcacca acaggtagaa gcgcgatcgc tggttttggc aacaccggct 900
tacgtaacag ccaacttgct caaatcctac ccccatgtca attcccagca gcaacaagcc 960
atccaagcct tggaaagcat tccctacccg cccgtagctt gtgtggtttt aggctatccc 1020
agcagtgcgt ttaaaaaaca acccttacat ggatttggca atttgattcc tcggggtcag 1080
ggcattcgca ctttgggcac catttggggc tctagtctgt ttccggggag ggcaccggaa 1140
ggttgggaat tgctgctgaa ttttattggc ggcaccaccg atccagaaat tgcgaacttg 1200
gatcaagaac aaattgccca actggtacat cgggatttgt gccagaccct gctgcgggaa 1260
gacaaacaac caaaagtact acaggttcac ttgtggaaac aggccatccc tcagtatact 1320
atcggtcacg ggagtcggct ggcagccatt gatgcggggg tgcgatcgct accggggttg 1380
tttttgtgca gcaactacag cgatggagtc gccatgggcg attgtgcccg acgcggctac 1440
gaactggcac cacgggtggc tgagtatttg caaccgggat cctataaatt cgtttga 1497
<210> 101
<211> 1491
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 22
<400> 101
atggttgtgg caacaccggg cgcggttgaa agcagtgtgg cgcgatcaaa cccgatcgac 60
ctgttgatcg tgggggcagg cttgacgggg ttgagcgcgg cccagcggtt tgtgcgccag 120
tcgccggggc gatcgtgcct ggtggtggag gcccaagatc gggtcggcgg caacatcacc 180
acgcgatcgg gcaatggctt tttgtgggaa gaaggcccca acagctttgc ccccacgccg 240
gagttgctgc aactggcggt ggaagtgggt ctcaaggatc agttggtgtt tgctgatggc 300
aagttgcccc gctttgtcta ttgggacggg cgcttgcagg caattcccat gagtccaggg 360
gcattttgga attcgacctt gttgagcgat cggggcaagg cgcggctgct gttgggggcg 420
gccggatttg tgccgccgat cctgggggcc acggtgcagg cccggggcgg cgaagaaacc 480
gtgcgggaat tttttacccg acatctgggc caagaaacca tggagcggat ggttgatccg 540
tttatttccg gggtctatgc cggcgatccc gatgccctga gcgcttcggc ggccttccgc 600
aagatggcgg ctatgcaggc agcgggcggg ggactggcgg ccggggcgat tcggacgttg 660
ctggccaagc ggcgggcggc caaaaccgcg cccccggctg acccgaattt gcccaagcca 720
aaatccggtg aactggggtc gttccgggaa ggcttgcaaa tgctgccgga agcggtggcc 780
aaggaactgg gcgatcgcgt gaagctgggc tggcgggtgg aggcgatcgc ccgatcaagc 840
aatggcgact acaccgtgga cttggcaacc cccgatgggc cgcgccaaat cacgactcgg 900
gcgatcgtcc tggccacccc cgctccagcc acggcgaacc tgttgcaacc cctggctccg 960
gccgccagcg ccgccctgca cgcaattccc tatccggcgg tagcttgcgt gattttggcc 1020
tatcccgaaa cggcctttgc ccaatccctg cgcggctttg gcaacttgat cccccgtagc 1080
ttggggctgc aaaccttggg cacgatttgg gcttctagcc tgtttgcggg ccgtgcgccc 1140
cagggttggg cccacctgat caacttcatc ggtggcgcgc aaaatcctgg gctaatcaac 1200
aaaaccgagg cggaaattgc agatatcgtc catggggatg tgcggcgcat tctgctgaag 1260
gaagacgtag ctcccaaggt gctgtcggtg aagctgtggc ggcgggcgat tcctcagtac 1320
acgatcggcc atgccgatcg cctggccaca ctgcatcggg aattggccgc ctggccgggg 1380
ctgttccctt gcagcaacta cgaaggcggt gtggccctgg gtgactgtgt gcgccacggt 1440
tgggatcaag cggctgaggt cgatcgctac ctggcccaac tgcccgttta g 1491
<210> 102
<211> 1446
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 23
<400> 102
atgacgaacc gagtagacac cttgattgtg ggtgctggta ttaccggctt gagtctggcc 60
cacgcgctgc acaaggaagc aaagactgga gaacccctga agattttagt tgctgagagt 120
cagggacgtg tgggcgggaa tatcacaact tctacagggc atgggtttct gtgggaggag 180
ggcccgaata gtttttcgcc gacgccggaa ttgatgaagt tggctgtgga tgtggggctg 240
aagcaggagt tgatgtttgc cgatcgccaa ttgcctcgtt ttgtatattg gcaaaataag 300
ctgcaaccgg tgccgatgac tcctggggcg atgattcagt ctgggttgct gagttttccg 360
gggaaactgc gggctctgtt cggggctttg gggtttgtgg ctccggtaat gggtgctaca 420
ctttcgcagc agggtgagga ggaaactgtt tctcaatttt tccggcgaca tttgggtacg 480
gaagtgatgc agcgattggt ggagcccttt gtgtctgggg tttatgccgg cgatcctcaa 540
caacttagcg ccgcggcggc ttttgggcgg gtgacaaaga tggctgatgc tggtggcggg 600
ctggtggccg gggcgctgct ttctgccagg aaacggccca aaaaaatgcc cccagacccg 660
aatattcctc agactaagcc gggggagctg ggttcgttta aaggggggct gatggctctg 720
ccagaggcga tcgctgcttc tttgggcgat cgcctgaaac tgaattggca tctgactggt 780
ttgcaccgta ctgaaaataa aacctatatt gcggaattct caacccccca tgggccgcaa 840
caaattgaga ctcggactgt ggtgctgaca acgcctgctt atgtggcagc ggatttgttg 900
caatctctgc aaccggaagt tagcagcact ttacaaggtt ttacttaccc tactgttgcc 960
tgtgtggtct tagcatatcc gctgtcggat gtcaagggta aattggtggg atttggaaat 1020
ttaattccga gggggcaggg aattcgcact ctgggaacga tttggacttc gagtttattt 1080
gccgatcgcg cgccggcggg ttggcaaact ctgacgagtt acatcggtgg ggctacggat 1140
tcgggtattg gcaatcttga ccccgaagaa attgtggccg aggtgcatcg agatttgtct 1200
cgcatcttgc tcaaaccgaa tgtgccacag ccgaaggtgt tggcggtgaa gctctggaaa 1260
caggcgattc ctcagtataa tttggggcat ctcgatcgcc tccaacagat cgatcgaggc 1320
ttaaaatctt tgccagggat gtatttgtgt agtaattacg ttggcggggt ggctttggga 1380
gattgtgtgc ggctgggttt ggaaaaggcg atcgcagtta gcaaatattt acaagaaacc 1440
agctaa 1446
<210> 103
<211> 1446
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 24
<400> 103
atgacgaacc gagtagacac cttgattgtg ggtgctggta ttaccggctt gagtctggcc 60
cacgcgctgc acaaggaagc aaagactgga gaacccctga agattttagt tgctgagagt 120
cagggacgtg tgggcgggaa tatcacaact tctacagggc atgggtttct gtgggaggag 180
ggcccgaata gtttttcgcc gacgccggaa ttgatgaagt tggctgtgga tgtggggctg 240
aagcaggagt tgatgtttgc cgatcgccaa ttgcctcgtt ttgtatattg gcaaaataag 300
ctgcaaccgg tgccgatgac tcctggggcg atgattcagt ctgggttgct gagttttccg 360
gggaaactgc gggctctgtt cggggctttg gggtttgtgg ctccggtaat gggtgctaca 420
ctttcgcagc agggtgagga ggaaactgtt tctcaatttt tccggcgaca tttgggtacg 480
gaagtgatgc agcgattggt ggagcccttt gtgtctgggg tttatgccgg cgatcctcaa 540
caacttagcg ccgcggcggc ttttgggcgg gtgacaaaga tggctgatgc tggtggcggg 600
ctggtggccg gggcgctgct ttctgccagg aaacggccca aaaaaatgcc cccagacccg 660
aatattcctc agactaagcc gggggagctg ggttcgttta aaggggggct gatggctctg 720
ccagaggcga tcgctgcttc tttgggcgat cgcctgaaac tgaattggca tctgactggt 780
ttgcaccgta ctgaaaataa aacctatatt gcccaattct caacccccga tgggcctcaa 840
caaattgaga ctcggactgt ggtgctgaca acgcctgctt atgtggcagc ggatttgttg 900
caatctctgc aaccggaagt tagcagcact ttacaaggtt ttacttaccc tactgttgcc 960
tgtgtggtct tagcatatcc gctgtcggat gtcaagggta aattggtggg atttggaaat 1020
ttaattccga gggggcaggg aattcgcact ctgggaacga tttggacttc gagtttattt 1080
gccgatcgcg cgccggcggg ttggcaaact ctgacgagtt acatcggtgg ggctacggat 1140
tcgggtattg gcaatcttga ccccgaagaa attgtggccg aggtgcatcg agatttgtct 1200
cgcatcttgc tcaaaccgaa tgtgccacag ccgaaggtgt tggcggtgaa gctctggaaa 1260
caggcgattc ctcagtataa tttggggcat ctcgatcgcc tccaacagat cgatcgaggc 1320
ttaaaatctt tgccagggat gtatttgtgt agtaattacg ttggcggggt ggctttggga 1380
gattgtgtgc ggctgggttt ggaaaaggcg atcgcagtta gcaaatattt acaagaaacc 1440
agctaa 1446
<210> 104
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 25
<400> 104
atggatgtgt tgattgtcgg cgcgggttta acgggcttga gcgctgccca ccgcttggtt 60
aatcacccgg gcaaccgagg gcgatcgacc ctggtggtgg aagcgcaaaa ccgtgtgggc 120
ggcaacatca ccacccgatc ggccgatggg ttcctgtggg aagaggggcc caacagcttc 180
gcccccacgc cagagctgct gaaactcgcc gtggaagtgg ggttgaagga tgagctggtg 240
tttgcggatg gcaagctgcc ccgatttgtc tactgggacg gtcggttaca agccattccc 300
atgagtcccg gcgcattttg gaattccacc ttgctgagcg atcggggcaa agccagactg 360
ctgttaggtg cggcgggttt tgtgcccccg atcgtgggtg cagccatttc ggcaaggggc 420
ggcgaagaaa ccgtccgtga attcttcact cgccacctgg gtcaagaagc catggagcgc 480
ctcgtggatc cctttatctc gggggtttat gccggagatc ccaatgccct cagtgcttcg 540
gcggccttcc gcaaaatggc cgccatgcaa gcggccggtg gtggcttggg agccggggcg 600
gcgcggattc tctgggccaa acgccaagcg gccaaacaag cacccccagc cgatccccgc 660
ttacccaagc ccaagtccgg ggagttggga tccttccgcg agggactgca agctttgccg 720
gaagcggtgg caagcggttt gggcgatcgg gtcaagttgg gttggcaggt ggaagcgatc 780
gcccgtgatc cccaaggcgt ttaccaggtt gcgatcgcca gccccgatgg cccccaacag 840
atcacggctc gatcgatcat tctggccacc ccggctccgg tcacggctca actgttggaa 900
cccctcgccc cccgagccag caccgccctc aacgccattc cctaccccgc cgttgcctgc 960
gtaattttgg cctatcccga aaccgccttt tcccagtccc tgcgcggctt tggcaacctg 1020
attccccgca gcctgggcct gcaaactcta ggaaccattt gggcttccag cctgtttgcg 1080
ggacgcgctc cccaaggttg ggcccaccta atcaacttca ttggcggcgc acaaaacccc 1140
accctgatcc acaaaaccga agacgaaatc gcccaaatgg ttcatgcgga cgtgcggcgg 1200
attttgctga aacaggacgt gccgcccaag gtgctgtcgg tcaaactctg gcggcgggcg 1260
atcccgcaat acacgatcgg gcatgggtct cgcctggcca ccttgcacca ggagcttcag 1320
cattggcctg gcctctttgc ttgcagcaac tatgaaggcg gcgtagcgct gggtgattgc 1380
gtgcgccatg gttgggaaca ggccgacgcg atcgagcagt tcctcggcag tcgagtctag 1440
1440
<210> 105
<211> 1473
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 26
<400> 105
atgaattcca ccgatttagc caatccatct actacccttg ataccttaat cgtcggtgca 60
gggattacgg gtcttagtct cgcccaagca cttcagcaag accgcaagcg cggtcgccaa 120
tcagggcaga ttttattgac agaaagtcaa gaaagggtag gggggcgcat tgttacacaa 180
tcgcaagacg gatttctctg ggaagaaggt cccaatagtt gcttaccaac ccctgagttt 240
ctcaagttgg cggtggatgt gggattaaaa gatgagttgg tgctggctga ccgtcgtctt 300
cctcggtatg tttatttaca gggagagtta atcccagtgc cgatgagtcc gccagcattt 360
tttcagacga agttactcag cgattggggt aagttacgcg cgatcgccgg ggctttgggt 420
tttgtgcctc ccgctatcgg tgccacccta tccgcccaag gggatgaaga aaccgtagcc 480
cagttctttg gtcgtcacct ggggcaggaa gtcttggaac gtctggtaca gccctttgtg 540
tctggagtct atgcgggaga tcccaatcaa ctcagtgcca gcgccgcttt tgggaaagtc 600
actaaaatgg ccgatatcgg cggtggactg gcggctgggg cgattttatc tttagcgaaa 660
aatgggagag cgaaaaagac ggtggatccg agtctgccca aagtccagcg cggggaatta 720
gcttcatttc gtcaaggtct ggaagcttta cccaaggcga tcgcggctca attgggcgat 780
gtagtcaagt taggctggca tttaatccaa attaaaccca cagaacacca aacttatctg 840
gccgaatttt ccacccccca aggtccagcc agaattgaaa ctcgcagtat ggttctgacc 900
acccccagct atgtctccgc tgacttgtta gccaatcttt gcccagtggc gagtcaaggg 960
ttagccaaaa ttccttatcc agcggtcgct tgcgtggtct taggctatcc tgaaagcgcc 1020
tttaaaacca gtgtatcatc gggttttggc aacttaatcc ccagaggtca aggaatacgc 1080
accttgggta cgatctggtc ttcgagttta tttcccaatc gcgctcccgc tggatggcgc 1140
ttattgctta attttatcgg tggcaccacg gatctggcga tcgctcagtt aagccaagaa 1200
gaaatcgtcc aaatcgtaca cagagatctg caaaaaaccc tgctcaaaca ggacatccca 1260
ccgaaagtcc tggcggtgca tttatggaag cgggcaattc cccaatatcc cctcggccat 1320
catcaaaacc tggcacaaat tcatcactct ttgcaacaac ggcctggatt atttttatgt 1380
ggcaactata cggatggggt tgccgtaggc gactgtattc gccgtggcca agaatgtgcc 1440
gctgacgtgg tgaaatattt agatcaaggc taa 1473
<210> 106
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 27
<400> 106
atggatgtgt tgattgtcgg cgcgggttta acgggcttga gcgctgccca ccgcttggtt 60
aatcacccgg gcaaccgggg gcgatcgacc ctggtggtgg aagcgcaaaa ccgtgtgggc 120
ggcaacatca ccacccgatc ggccgatggg ttcctgtggg aagaggggcc caacagcttc 180
gcccccacgc cagagctgct gaaactctcc gtggaagtgg gtttgcagga tgagctggtg 240
tttgcggatg gcaagctccc ccgatttgtc tattgggacg gtcggttaca agccattccc 300
atgagtcccg gcgcattttg gaattccact ctgctgagcg atcggggcaa agccagactg 360
ctgctaggcg cggcgggttt tgtgcccccg atcgtgggtg cagccatttc agcaaggggc 420
ggtgaagaaa ccgtccgtga attcttcact cgccacctgg gccaagaagc catggagcgc 480
ctcgtggatc cctttatctc gggggtttat gccggagatc ccaacgccct cagcgcttcg 540
gcggccttcc gcaaaatggc cgccatgcaa gcggccggtg gtggcttggg agccggagct 600
gcgcggattc tctgggccaa acgccaagcg gccaagcaag cgcccccagc cgatccccgc 660
ttacccaagc ccaagtccgg ggagttggga tccttccgcg agggactgca agccttgccg 720
gaagcggtag caagcggttt gggcgatcgg gtcaagttgg gttggcaggt ggaggcgatc 780
gcccgtgatc cccaaggcac ttaccaggtt acgatcgcca gccccgatgg cccccaacgg 840
atcaccgcgc gatcgatcat tctggctacc ccggctcccg tcacggccca actgctcgaa 900
cccctcgccc cccgagccag caccgccctc aacgccattc cctaccccgc cgttgcctgc 960
gtgattttgg cctatcccga aaccgccttt gcccagtccc tgcgcggctt tggcaacctg 1020
attccccgca gcctgggcct gcaaacccta ggaaccattt gggcttccag cctgtttgcg 1080
ggacgcgctc cccaaggttg ggcccacctg atcaacttca ttggcggcgc acaaaacccc 1140
accctgatcc acaaaaccga agacgaaatc gcccagatgg ttcatgcgga cgtgcggcag 1200
attttgctga aacaggacgt accgcccaag gtgctgtcgg tcaagctctg gcgacgggcg 1260
atcccgcaat acacgatcgg gcatgggtct cgcttggcca ccttgcacca ggagttgcaa 1320
cattggcctg gcctctttgc ttgcagcaac tatgaaggcg gcgtagcgct gggtgattgc 1380
gtgcgccatg gttgggaaca ggccgacgcg atcgagcagt tcctcggcag tcgagtctag 1440
1440
<210> 107
<211> 1437
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 28
<400> 107
atgactgaag ttttagatgt cttagttgtt ggcgcaggta ttagtgggtt aagtttagcc 60
catgcactga caaaattagg taataattcc ccgttaaaga ttttagttgc agaaagccaa 120
aatcgagtgg ggggaaatat cacaacagtt tctttaggag actttctttg ggaagaaggg 180
ccaaatagtt tttctccgac accggaattt ttaaagttgg ctgtagatgt tggcctgaag 240
caggagctag tttttgccga tcgcaaactg cctcgctatg tttattggaa tggtcaatta 300
ttaccagtgc ccatgagtcc aacagctatg ctacagtcta aattactgag tgatgctggt 360
aagctacgcg ctttagttgg cgctttgggt tttgtctcac ctgccattgg taatttatca 420
ggacaaggag gagaagaaac tgtatcccaa tttttccaga gacatcttgg tcctgaagta 480
atgcagcgac tggtggaacc tttcgtttct ggtgtttatg caggagaccc tagtcaactg 540
agtgcttctg ctgcttttgc taaagtggct cgaatggctg acttaggtgg tggacttgta 600
gctggtgctt tgctgtctgc taaaaaaaat cgtaagttta aggttgctcc cgaccctaat 660
attcctaaga ctaagactgg tgaattgggt tcttttcgag gtggtttgga ggcattacca 720
aaagcgatcg cctcttattt aggagaagca gttaaattaa attggcatct tactaatatt 780
cgtcggactg aacaacaaac ttatatagca gaattttcta ctcccaatgg tcctgagcaa 840
atagaaactc gtactatttc cctttctact cccgctcgtg tttgtgcaga attattcaaa 900
actttgcaac cggaaattgc ttctatattt aatgaatttt attatcctcc tgttgcctgt 960
gtagtcttgg catatcctga tacttctatc aaggttaaga tagatggctt tggtaatttg 1020
attcctagag gtcaaggtat ccggactctt ggtagtattt ggtcttctac tttattttct 1080
ggtagaacac ctccaggatg gcaaattttt actaatttta ttggtggtgc aacagacccg 1140
gaaattgcga acttagatag tgaagcgata gtgcaacagg tacatcaaga tctttgtcaa 1200
actctgttaa aacagaatgc tgaacaacca aaagttcttg cggtgcattt atggtctcgt 1260
gctattcctc aatataattt aggccataat tctaaattgg aggagattaa taatggctta 1320
aaatctttgc ctggtctata tctgtgcagt aattatattg gtggtatagc tttgggagat 1380
tgtgttcgcc gtggcacaga agtagcgact gaaatttatc aaggtttgca gacttga 1437
<210> 108
<211> 1435
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 29
<400> 108
atgactgaag ttttagatgt cttagttgtt ggcgcaggta ttagtgggtt aagtttagcc 60
catgcactga caaaattagg taataattcc ccgttaaaga ttttagttgc agaaagccaa 120
aatcgagtgg ggggaaatat cacaacagtt tctttaggag actttctttg ggaagaaggg 180
ccaaatagtt tttctccgac accggaattt ttaaagttgg ctgtagatgt tggcctaaag 240
gaggagctaa tctttgccga tcgcaaactg cctcgctatg tttattggaa tggtcaatta 300
ttgccagtac ctatgagtcc aaaagctatg ctacagtcta agttactgag tgatggtggt 360
aagttacgcg ccctagttgg tgctttgggt tttgtatcgc ctgctattgg cgatctgtca 420
gagcaagggg gagaagaaac tgtatcccaa tttttccaga gacatcttgg tgctgaagtg 480
atgcagcgac tggtggaacc ttttgtttct ggtgtttatg caggagatcc tggtcaactg 540
agtgctaatg cggcttttgc taaagtggct cgaatggctg acttaggtgg tggacttgtc 600
gctggtgctt tgttgtctgc caaaaaaaat cgtaagttca aggttgctcc cgatcctaat 660
attcctaaga ctaaaactgg cgaattgggt tcttttcgag gtggtttgga ggcattacca 720
aaagcgatcg cttcttattt aggagaagca gttaaattaa attggcatct tactggtatt 780
cgccgcactg aacaacaaac ttatatagca gaattttcga ctcccaatgg tactgagcaa 840
atagaaactc gtactatttc tctttctact cctgctcgtg tttgttcaga gttattcaca 900
actttgcaac ctgaaattgc ttctatattt aatgagtttt attatcctac tgttgcctgt 960
gtagtcttgg catatcctga tacttctatc aaggttaaga tagatggctt tggtaatttg 1020
attcctagag gtcaaggtat ccggactctt ggtagtattt ggtcttctac tttattttct 1080
ggtagaacac ctccaggatg gcaaattttt actaatttta ttggtggtgc aacagacccg 1140
gaaattgcga acttagatag tgaagcgata gtgcaacagg tacatcaaga cctttgtcaa 1200
actctgttaa aacagaatgc tgaacaacca aaagttcttg cggtgcattt atggtctcgt 1260
gctattcctc aatataattt aggccataat tctaaattgg aggagattaa taatggctta 1320
aaatctttgc ctggtctata tctgtgcagt aattatattg gtggtatagc tttgggagat 1380
tgtgttcgca gtgggacaga agtagctact aagatttatc aaggtttaca agtga 1435
<210> 109
<211> 1473
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 30
<400> 109
atgtctttca ccgatctggc cactccatct cacccccttg atacgctcgt tattggggca 60
ggaattagcg gtttaagtct tgcctttacc ttgcaacaac ggcagaccca agtattagtc 120
tgcgaaagtc aaaaccgcat tggggggaat atcacaacgg gtcaggcgga tggttttctg 180
tgggaggaag ggccgaacag tttcgcgccg acacccgctc ttttaaggct aattgtcgat 240
gctggattag agaaagacat gattcttgcc gaccggcgct taccgcgttt tgtgtacagg 300
caagggcgct tgcaagcgat tcccatgagt cccccggcgg cgattgggac gccgttactc 360
agttggccgg gtaaactcag ggctggattg ggtgccatcg gttttgtccg ccccccgttg 420
ggcgagcagt tttctcaaca aggctcagaa gagacggtgg ctcaattttt ccagcgccac 480
ctcggtgaag aggtgatgga aaggctggta acgccgtttg tctctggcgt gtatgccgga 540
gatgtgaatc aattgagtgc ggcggcagcc tttcggagaa ttgctcagtt agagggcgtt 600
ggcggcggtt tagtggccgg ggcgattctc tcgcggatga aggccaaatc tcagccgcaa 660
gtcccggtcg atccaagctt acctaaaacg cgacctgggg agttagggtc atttcaacaa 720
gggttacaaa tgctacccca agcgctggcg gccaaattag gcgatgcggt gcggttgaat 780
tggcggttgc tgcaaattac cccaacaacg cgccaaagct atattgcaga attcggtacc 840
ccagagggaa cgcagattgt agaagctcgc catgtggtgt tgacaacgcc tgcttatatt 900
accgcagaga ttgtagaggc gatcgcgccc cttgccagcc aagctttgcg agcgattgag 960
tatcccccgg tggcaaacgt cgtcttagcc tacccagaat cagccttgaa gcagccttta 1020
aagggatttg ggcatttgat tccgcgtagc gaaggaatcc gcactctagg gacaatctgg 1080
tcgtctagcc tgtttcccgg acgagtcccc ccaggctggc agttactcac caattatatt 1140
ggcggcgcaa ccgatccagg cattttagac ttagaccgcg attcgcgaag caatctcgac 1200
gggaatccca ttgctcaagc cgttcaccaa gatatccgcc gcatcctcct ccagcaagag 1260
gttcagccca aggtgctagc ggttcatctg tggaagcggg cgattcccca atataatcta 1320
ggtcatcacc agcgcttgca aaccattttc caaagtttgg ccccgttccc cggtttgcat 1380
atttgcagca actataccga tggggtagcc ctcggagatt gcgtccgcag agcgcaagaa 1440
ctcgctgata tcttaaccca aaaaactcgg tag 1473
<210> 110
<211> 1470
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 31
<400> 110
atggaagcgg cagtgaatcc tgcaactcct gaacccctta acgcggaggt ggtggtcatt 60
ggggcgggca tcagcggcct gacgctggcc tggcgcctgc agcagggttt atcggctagg 120
gggggatccc ctcaagcggt gctcctggcg gaggccagct cccgcgtcgg cggctgcatt 180
tccacccagt ccaaagacgg ctactgctgg gaggaaggcc ccaacagctt tacccccacc 240
cctgccctgc tcaacctcat tgctgaggtg ggcctgaccg accaactggt gctggccgat 300
gccaagctgc cccgctacat ctactgggag aaagagctgc tgccggtgcc gcttagcccc 360
gcggcagccc tgagctcccg gctgctcagt gtgggcggca agctgcgggc tctgcagggc 420
ctgctggggt ttgtcccgcc gccgccagga cgcgaggaaa cggtgcggca gtttttccgg 480
cgacaactgg gatccgaagt ggccgagcgc ctggtggagc cctttacctc gggggtttat 540
gcgggggatc cggatcagct cagcgccgtg gcggctttcc ctcgcgtggc gggcctggag 600
gagcgctatg gcagcctctt tgccggcgcc ctgcaggctc tgcgccaaag accccagcct 660
tctcctgcgg ccatccaacc cccgcccaaa cggggccaac tgggcaatct gaggcagggg 720
ctgcagcagt tgccagaggc cctagcccaa aagctgggcg acagcctgcg gctgggctgg 780
cgagcggtgc agcttaagcg ggagggagag ctctattggg tgggctttga gacgccagag 840
ggatcccgct gggtggctgc ccgccaggtg gtgctggccc tccccgccta cgaggctgcc 900
gcgctgctac aggagctcaa ccccccggcc agccagctct tggccgagat cctctatccg 960
ccggtggcgg tggtggctct ggcctatccc caagaggccc tgccccagcc gctgcggggc 1020
tttggccacc tcatcccccg ttcccagggc ctgcgcaccc tgggcacgat ctgggcctcc 1080
tgcctttttc cagagcgggc tccccagggc taccacagct tcctcagctt cctgggcggc 1140
gccaccgatg cggccttggc ccgccggcgg gggatcccgc ccatccctgc gctgtctcct 1200
gaggagcgag cgcaaattgc ccacgccgag cttagccagg ttctgctcac ccgccgcgtc 1260
gagcccatct acctcgggga acgcctatgg ccccgcgcca tcccccagta cacccttggc 1320
caccggcaac gcatagccca ggtgcaggcc cacctggcct cccagacgcc agggatctgg 1380
gtatgcgcca actacctcga tggcgtggcc ctgggcgatt gcgtgcggcg ggccgaggcg 1440
ctggcgcagc agctgttgtc ccaggtctag 1470
<210> 111
<211> 1470
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 32
<400> 111
atggaagcgg cagtgaatcc tgcaacccct gaacccctta acgcggaggt ggtggtcatt 60
ggggcgggca tcagcggcct gacgctggcc tggcgcctgc agcagggttt atcggctagg 120
gggggatccc ctcaagcggt gctcctggcg gaggccagct cccgcgtcgg cggctgcatt 180
tccacccagt ccaaagacgg ctaccgctgg gaggaaggcc ccaatagctt tacccccacc 240
cctgccctgc tcaacctcat tgctgaggtg ggcctgaccg accaactggt gctggccgat 300
gccaagctgc cccgctacat ctactgggaa ggagcgctgt tgccggtgcc tctgagcccc 360
gcggcagccc tgggatcacg gctgctcagc gtgggcggca agctgcgggc tctgcagggc 420
ctgctggggt ttgtcccgcc gccgccagga cgcgaggaaa cggtgcggca gtttttccgg 480
cgacaactgg gatccgaagt ggccgagcgc ctggtggagc ccttcacctc aggagtctat 540
gccggcgacc cagatcaact cagcgccgtg gcggctttcc ctcgcgtggc gggcctggag 600
gagcgctatg gcagcctctt tgccggcgcc ctgcaggctc tgcgccaaag accccagcct 660
tctcctgcgg ccatccaacc cccgcccaaa cggggccaac tgggcaatct gaggcagggg 720
ctgcagcagt tgccagaggc cctagcccaa aagctgggcg acagcctgcg gctgggctgg 780
cgagcgctgc agctgaagcg ggcgggagag ctctattggg tgggctttga gacgccagag 840
ggatcccgct gggtggctgc tcgccaggtg gtgctggccc tccccgccta cgaggctgcc 900
gcgctgctac aggagctcaa ccccccggcc agccagctct tggccgagat cctctatccg 960
ccggtggcgg tggtggctct ggcctatccc caagaggccc tgccccagcc gctgcggggc 1020
tttggccacc tcatcccccg ttcccagggc ctgcgcaccc tgggcacgat ctgggcctcc 1080
tgcctttttc cagagcgggc tccccagggc taccacagct tcctcagctt cctgggcggc 1140
gccaccgatg cggccttggc ccgccagcaa gggatcccac ccatccctgc gctgtctcct 1200
gaggagcgag cgcaaattgc ccacgccgag cttagccagg ttctgctcac ccgccgcgcc 1260
gagcccgtct acctgggcga acgtctctgg ccccgcgcca tcccccagta cacccttggc 1320
caccggcaac gcatagccca ggtgcaggcc cacctggcct cccagacgcc ggggatctgg 1380
gtgtgtgcca actacctgga tggcgtggcc ctgggcgatt gcgtgcggcg ggccgaggcg 1440
ctggcgcagc agctgttgtc ccaggtctag 1470
<210> 112
<211> 1470
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 33
<400> 112
atggaagcgg cagtgaatcc tgcaacccct gaacccctta acgcggaggt ggtggtcatt 60
ggggcgggca tcagcggcct gacgctggcc tggcgcctgc agcagggttt atcggctagg 120
gggggatccc ctcaagcggt gctcctggcg gaggccagct cccgcgtcgg cggctgcatt 180
tccacccagt ccaaagacgg ctaccgctgg gaggaaggcc ccaatagctt tacccccacc 240
cctgccctgc tcaacctcat tgctgaggtg ggcctggccg accaactggt gctggccgat 300
gccaagctgc cccgctacat ctactgggaa ggagcgctgt tgccggtgcc tctgagcccc 360
gcggcagccc tgggatcacg gctgctcagc gtgggcggca agctgcgggc tctgcagggc 420
ttgttggggt ttgtcccgcc gccgccagga cgcgaggaaa cggtgcggca gtttttccgg 480
cgacaactgg gatccgaagt ggccgagcgc ctggtggagc ccttcacctc aggagtctat 540
gccggcgacc cagatcaact cagcgccgtg gcggctttcc ctcgcgtggc gggcctggag 600
gagcgctatg gcagcctctt tgccggcgcc ctgcaggctc tgcgccaacg accccagcct 660
tctcctgcgg ccatccaacc cccgcccaaa cggggccaac tgggcaatct gaggcagggg 720
ctgcagcagt tgccagaggc cctagcccaa aagctgggcg acagcctgcg gctgggctgg 780
cgagcgctgc agctgaagcg ggcgggagag ctctattggg tgggctttga gacgccagag 840
ggatcccgct gggtggctgc tcgccaggtg gtgctggccc tccccgccta cgaggctgcc 900
gcgctgctac aggagctcaa ccccccggcc agccagctct tggccgagat cctctatccg 960
ccggtggcgg tggtggctct ggcctatccc caagaggccc tgccccagcc gctgcggggc 1020
tttggccacc tcatcccccg ttcccagggc ctgcgcaccc tgggcacgat ctgggcctcc 1080
tgcctttttc cagagcgggc tccccagggc taccacagct tcctcagctt cctgggcggc 1140
gccaccgatg cggccttggc ccgccagcaa gggatcccac ccatccctgc gctgtctcct 1200
gaggagcgag cgcaaattgc ccacgccgag cttagccagg ttctgctcac ccgccgcgcc 1260
gagcccgtct acctgggcga acgtctctgg ccccgcgcca tcccccagta cacccttggc 1320
caccggcaac gcatagccca ggtgcaggcc cacctggcct cccagacgcc ggggatctgg 1380
gtgtgtgcca actacctgga tggcgtggcc ctgggcgatt gcgtgcggcg ggccgaggcg 1440
ctggcgcagc agctgttctc ccaggtctag 1470
<210> 113
<211> 1470
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 34
<400> 113
atggaagcgg cagtgaatcc tgcaacccct gaacccctta acgcggaggt ggtggtcatt 60
ggggcgggca tcagcggcct gacgctggcc tggcgcctgc agcagggttt atcggctagg 120
gggggatccc ctcaagcggt gctcctggcg gaggccagct cccgcgtcgg cggctgcatt 180
tccacccagt ccaaagacgg ctaccgctgg gaggaaggcc ccaatagctt tacccccacc 240
cctgccctgc tcaacctcat tgctgaggtg ggcctggccg accaactggt gctggccgat 300
gccaagctgc cccgctacat ctactgggaa ggagcgctgt tgccggtgcc tctgagcccc 360
gcggcagccc tgggatcacg gctgctcagc gtgggcggca agctgcgggc tctgcagggc 420
ttgttggggt ttgtcccgcc gccgccagga cgcgaggaaa cggtgcggca gtttttccgg 480
cgacaactgg gatccgaagt ggccgagcgc ctggtggagc cctttacctc gggggtttat 540
gcgggggatc cggatcagct cagcgccgtg gcggctttcc ctcgcgtggc gggcctggag 600
gagcgctatg gcagcctctt tgccggcgcc ctgcaggctc tgcgccaaag accccagcct 660
tctcctgcgg ccatccaacc cccgcccaaa cggggccaac tgggcaatct gaggcagggg 720
ctgcagcagt tgccagaggc cctagcccaa aagctgggcg acagcctgcg gctgggctgg 780
cgagcgctgc agctgaagcg ggcgggagag ctctattggg tgggctttga gacgccagag 840
ggatcccgct gggtggctgc ccgccaggtg gtgctggccc tccccgccta cgaggctgcc 900
gcgctgctac aggagctcaa ccccccggcc agccagctct tggccgagat cctctatccg 960
ccggtggcgg tggtggctct ggcctatccc caagaggccc tgccccagcc gctgcggggc 1020
tttggccacc tcatcccccg ttcccagggc ctgcgcaccc tgggcacgat ctgggcctcc 1080
tgcctttttc cagagcgggc tccccagggc taccacagct tcctcagctt cctgggcggc 1140
gccaccgatg cggccttggc ccgccggcgg gggatcccgc ccatccctgc gctgtctcct 1200
gaggagcgag cgcaaattgc ccacgccgag cttagccagg ttctgctcac ccgccgcgcc 1260
gagcccgtct acctgggcga acgtctctgg ccccgcgcca tcccccagta cacccttggc 1320
caccggcaac gcatagccca ggtgcaggcc cacctggcct cccagacgcc ggggatctgg 1380
gtgtgtgcca actacctgga tggcgtggcc ctgggcgatt gcgtgcggcg ggccgaggcg 1440
ctggcgcagc agctgttctc ccaggtctag 1470
<210> 114
<211> 1470
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 35
<400> 114
atggaagcgg cagtgaatcc tgcaactcct gaacccctta acgcggaggt ggtggtcatt 60
ggggcgggca tcagcggcct gacgctggcc tggcgcctgc agcagggttt atcggctagg 120
gggggatccc ctcaagcggt gctcctggcg gaggccagct cccgcgtcgg cggctgcatt 180
tccacccagt ccaaagacgg ctaccgctgg gaggaaggcc ccaatagctt tacccccacc 240
cctgccctgc tcaacctcat tgctgaggtg ggcctgaccg accaactggt gctggccgat 300
gccaagctgc cccgctacat ctactgggaa ggagcgctgt tgccggtgcc tctgagcccc 360
gcggcagccc tgggatcacg gctgctcagc gtgggcggca agctgcgggc tctgcagggc 420
ctgctggggt ttgtcccgcc gccgccagga cgcgaggaaa cggtgcggca gtttttccgg 480
cgacaactgg gatccgaagt ggccgagcgc ctggtggagc ccttcacctc aggagtctat 540
gccggcgacc cagatcaact cagcgccgtg gcggctttcc ctcgcgtggc gggcctggag 600
gagcgctatg gcagcctctt tgccggcgcc ctgcaggctc tgcgccaaag accccagcct 660
tctcctgcgg ccatccaacc cccgcccaaa cggggccaac tgggcaatct gaggcagggg 720
ctgcagcagt tgccagaggc cctagcccaa aagctgggcg acagcctgcg gctgggttgg 780
cgagcgctgc agctgaagcg ggcgggagag ctctattggg tgggctttga gacgccagag 840
ggatcccgct gggtggctgc ccgccaggtg gtgctggccc tccccgccta cgaggctgcc 900
gcgctgctac aggagctcaa ccccccggcc agccagctct tggccgagat cctctatccg 960
ccggtggcgg tggtggctct ggcctatccc caagaggccc tgccccagcc gctgcggggc 1020
tttggccacc tcatcccccg ttcccagggc ctgcgcaccc tgggcacgat ctgggcctcc 1080
tgcctttttc cagagcgggc tccccagggc taccacagct tcctcagctt cctgggcggc 1140
gccaccgatg cggccttggc ccgccggcgg gggatcccgc ccatccctgc gctgtctcct 1200
gaggagcgag cgcaaattgc ccacgccgag cttagccagg ttctgctcac ccgccgcgcc 1260
gagcccgtct acctgggcga acgtctctgg ccccgcgcca tcccccagta cacccttggc 1320
caccggcaac gcatagccca ggtgcaggcc cacctggcct cccagacgcc ggggatctgg 1380
gtgtgtgcca actacctgga tggcgtggcc ctgggcgatt gcgtgcggcg ggccgaggcg 1440
ctggcgcagc agctgttctc ccaggtctag 1470
<210> 115
<211> 1458
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 36
<400> 115
atgagtgaac cagcgaatcc atctacctta ctcgataccc tcgtcgtcgg tgcgggcatt 60
acgggcctga cgcttgcatt cgacttgcaa cagaaatcga gcggcaccgc ccattcagcg 120
aaagttctcg tcgccgaaag tcaagctcgg gtgggaggtc gcatcgttac cgcatcggga 180
gatggttttc tctgggaaga aggccccaac agttttgcgc cgaccccaga gttattacaa 240
ttagccgttg aagtcggctt aaaagataaa ttagtcttcg ccgacggcaa actccctcgc 300
ttcgtctact ggcagggaga attaatgccc gttcccatga gtccccctgc catcattagc 360
tcaaaactgc ttacttggcg ggggaaactg cgcgcattct tcggtgcttt ggggtttgtc 420
cccccggcaa tggccgatgt gggttccgag gaaactgtcg cctccttctt cgagcgccat 480
ttgggccgcg aagtgctgca gcgcttagtc gaaccgttcg tgtccggggt ctatgccgga 540
gatccgcgac agttaagcgc ccgagcggca tttgggcgtg tggcgcgcat ggcagaagca 600
gggggcggac tggttgcggg agcagtccgc acgagccgcc agaaaccgaa aacgaaagtc 660
accccagatc cgaacgtccc gcagcccaaa cggggacagc tcggttcttt tcgtcaagga 720
ttggcgactt tgcccgaggc gatcgccgat cgcctcggag acgcagtcca actcaactgg 780
cacttaattc gcgtgcgccc caccgaacga cacacctaca ttgccgagtt ttcgacgccg 840
gacggtccga agcgggtaga agcgcgcagt gtggtcttga ccaccccaac ttacgtgacg 900
gctgatttat tcgatcccct ccacggggag attgcccggg ccttacgggg atttgtctat 960
ccgtcagtgg cttgtgtggt gttgggatat cccacttcgg cgttgaaacg cccgttaaat 1020
ggttttggga atttgattcc ccgaaatcag gggattcgca ccctggggac gatttggtct 1080
tcgtccttgt tttccgggcg ggcgccggag ggttggaatt tgttgattaa ttttattggg 1140
ggcacggggg atccggcgat cgccgagtta gagaaagacg agatcgttcg ggtcgtgcat 1200
cgagatttac tcaaaacgct gctcgcgcga gatgtagacc cgaaggtgtt ggcagttcat 1260
ctctggaagc gggcaattcc ccaatattgc ttgggtcacc accagcgctg ggagcagatc 1320
gatcgcggct tgcaggagtt tcccggtctt tacttgtgtg ggaattacag tgacggggta 1380
gcggtgggcg attgcgttcg tcgcgcgcga gatcgcgctg cggagatccg ccaatatttg 1440
gcctcggcga cggtctaa 1458
<210> 116
<211> 1407
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 37
<400> 116
atgttagaca gtctgattgt gggggccggt ctcagtggct tgaccgttgc ccataccttg 60
cagcagcgcg atcgcaatat tctcgtcgcg gaagctagcc ccgatgtggg gggcaatatt 120
attagccgcc aacagggtga atttctctgg gaggagggcc caaacagttt tcaaccgaat 180
ccgaccctgc tcaaattggc ggtggatgtg ggactaaagg atgacctcgt ctttgccgat 240
cgcaaactgc cccgctgggt ctattggcaa ggtcgtctct tggctgtacc catgagtccg 300
gggactgcgg tgcgatcgcc cctgctgagt gtgccgggga aactgcgggc cctgttcggg 360
gcgctggggt ttgtgccgcc cttggtcgga agccacattc aggcccaagg cggcgatgaa 420
accatttggc aatttattaa ccgtcattta ggcccagaag tggccgagcg gctgatttcg 480
ccctttgtgt cgggggtcta tgctggggat gtccacgccc tgagtatggc ggcagccttt 540
cggaaaattt atcgcctcga aaccctgggc ggtggccttg tggcgggggc gatgcgatcg 600
cggcggcaag gtaaagccga tcgccccgct gttgatccca atctgcccac cacgaagccg 660
ggccagttgg ggtcatttcg cgagggcatg gtgatgctac ccaacgcgat cgctgctcga 720
ttgggcgatc gcctccgctg ccgctggacg ctgacccaga tcgaaccgtt ggaacagggc 780
tatcgcgccc attttgacac ccccgacggc ggccaaacca tcgagacgcg caccctcgtt 840
ttggcgattc ctgcccatcg agtggccccc ctcctcgcgc ccctgatgcc ggagttaagc 900
accacattac aagcgattcc ctatcctgcc gtagcctgta ccgtgatggc ctatcccaaa 960
acggcgttgg tgcgatcgct ccacggcttt ggcaacctca acccccgcag ccaaggtatc 1020
cgcacccttg gcaccatctg gtcatccacc ctcttccccg gtcgtgcgcc cgaaggttgg 1080
gtgatgttga gtagttttat cggtggctcc actgatccgg cggtggcgac gatggatgaa 1140
ggcgcgatcg cccaagccgt ccaccaagac ctcagtaaca ttctcgtcaa acccgacagc 1200
accccgaaag tcctcgccgt caaactctgg tcaaaagcga ttccccaata caccctaggg 1260
cattgcgatc gcctctccgt catggccgaa cacctcaaag cccaccccca cctcacccta 1320
tgcagcaact acaccgacgg tgtcgccctc ggtgactgca tccgacgcgg catcgaagcg 1380
ggagaagcga tcgatcagca actctaa 1407
<210> 117
<211> 1392
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 38
<400> 117
atggtagaga ctttaattgt cggagcagga attagtggtt tgaccactgc ttatcggtta 60
catgagcaac aacatgaggt gatggtagcc gaaaagaacg atcgagcagg gggaaacatt 120
accagcaaag ccagtggggg tttcctctgg gaagaaggac ccaatagctt ttctccgacc 180
ccagaattgc taaaactggc ggtagatgtc gggttaaaag atgagttcgt atttgccgat 240
ccgacgcttc cccgttatgt ttattggcag gggaaactac ttcctgttcc catgagtccc 300
ccatcggcgg taaaatcaca gttgttgagt ccattgggga aactgcgagc gttaacgggt 360
gcgatcgggt ttgttccccc aaaggtggcg actgaagaag aaacagtggc ggaatttttc 420
acccgtcatt tgggttcgga agtggcgcag cgattggtga gtccttttgt ttctggggtg 480
tatgctggag atgtggcaaa cttgagtgct tcggcagctt ttggacgagt gacgcaattg 540
gcggatgtgg gaggcggact ggttgctggg gcaattttat ctcgtggcaa gaaaaaaaag 600
gcaaccactg aggttgatcc agagattccc aaaacgcgat cgggagaatt aggatcattt 660
cgagaagggt tacagcaatt accaagctcg atcgcccgta aattaggaga ggcggtaaaa 720
ttcaattggg aactaaaaca gatttcccag acttcagaag cgggctacat tgccactttt 780
tccaccccag aaggagaaca aaacgtggaa gcgcagcaaa tcatcctcac gactcctgct 840
tatgttagcg ctccaatttt acaagactta tccccagaag cgagtcaagc gctgagagaa 900
atttattatc cgccagtagc ttgtgtggtt ctcgcgtatc ccgatgaagc cttcagtgtg 960
ccattagacg ggtttggtaa tctcaatccg cgtagtgaag gagtccgtac tttaggaaca 1020
atttggtctt ctactttgtt ttctggacgt actcctcaag gttggcagat tctgactaat 1080
tttatcggcg gcgcaactga tcctgaaatt gctcaactca gtgaagaaga gattgttcaa 1140
caagtccatc aagacttaca gaaaaccata gttaaaccga atacgacacc gaaaccccta 1200
gcggttcatt tatggtcgca agcgattcct caatataccc ttggacatct cgatcgaatc 1260
gcgagaatta aagagagttt gaaaccgttt tcggggctat ttttatcgag taactatctt 1320
gatggcgttg cgttaggaga ttgtgtgcgg cgcggggaag aaacagcagc ccagattctt 1380
aataaaaagt ag 1392
<210> 118
<211> 1386
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 39
<400> 118
atggtagaga ctttaattgt cggagcagga attagtggtt tgaccactgc ttatcggtta 60
catgagcaac aacatgaggt gatggtagcc gaaaagaacg atcgagcagg gggaaacatt 120
accagcaaag ccagtggggg tttcctctgg gaagaaggac ccaatagctt ttccccgacc 180
ccagaattgc taaaactggc ggtagatgtg gggttaaaag atgagttcgt atttgccgat 240
ccgacgcttc cccgttatgt ttattggcag gggaaactac ttcctgttcc catgagtccc 300
ccagcggcgg taaaatcgca actactgagt ccattgggga aactgcgagc gttaacgggt 360
gcgatcgggt ttgttccccc aaaggtggcg actgaagaag aaacagtggc ggaatttttc 420
acccgtcatt taggctcgga agtggcgcag cgattggtga gtccttttgt ttctggggtg 480
tatgctggag atgtggcaaa cttgagcgct tcggcagctt ttgcacgagt gacgcaattg 540
gcggatgtgg gaggcggatt ggttgctggg gcaattttat ctcgtggcaa gaaaaaaaag 600
gcaaccactg aggttaattc agagattccc aaaacgcgat cgggagaatt aggatcattt 660
cgagaagggt tacagcaatt accaagctcg atcgcccgta aattaggaga ggcggtaaaa 720
ttcaattggg aactaaaaca gatttcccag acttcagaag cgggctacat tgccactttt 780
tccaccccag aaggagaaca aaacgtggaa gcgcagcaaa tcatcctcac gactcctgct 840
tatgttaacg ctccaatttt acaagactta tccccagaag cgagtcaagc gctgagagaa 900
attgattatc cgccagtggc ttgtgtggtt ctcgcgtatc ccgatgaagc cttcagtgtg 960
ccattagacg ggtttggtaa tctcaatccg cgtagtgaag gagtccgtac tttaggaaca 1020
atttggtctt ctactttgtt ttctggacgt actcctcaag gttggcagat tctgactaat 1080
tttatcggcg gcgcaactga tcctgaaatt gctcaactca gtgaagaaga gattgttcaa 1140
caagtccatc aagacttaca gaaaaccata gttaaaccga atacgacacc gaaaccccta 1200
gcggttcatt tatggtcgca agcgattcct caatataccc ttggacatct cgatcgaatc 1260
gcgagaatta aagagagttt gaaaccgttt tcggggctat ttttatccag caactatctt 1320
gatggcgttg cgttaggaga ttgtgtgcgg cgcggggaag aaacagcagc ccagattctt 1380
agataa 1386
<210> 119
<211> 1404
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 40
<400> 119
atgggctccc acacccccga tattttgatt ctcggcgcag gcatcagtgg cctatccgct 60
gccttccgac tccaccaaca gcaacaagat ctattggtgg ccgagcgcgc cgagcgcgtt 120
ggcggcgtca tcaccacccg cgctcaagac ggctttcgct gggaagaagg ccccaacagc 180
ttcaccccct cccccgccct cctcaacctc atcgccgacg caggcattgc cgatcgcctc 240
ctgtgggcag atggcaagtt accccgcttc gtctacctcg aaggcaaact caccctcgtt 300
cccatgactc ccccagactt aatcaagtcc aacctgctca gctttggggc taagctgcgc 360
gccttattgg gcattctcgg ctttacagcc aaagcccccg acaaagaaga gaccgtcgaa 420
gaattctttg cccgccaact ggggcctcag gtggtcgagc gcttggtcgg ccccttcacc 480
tccggcgtct atgcaggcga cacgcagcaa ctgagcgcca cagccgcgtt ctccaaagtg 540
gcggatctag aacgtaaata cggcagcatt attgccggca tcatccgctc ccccaaatcc 600
cccaaacccc ccatctccgc aaaaatcgac cccctgccca aacgcggcca gctcggtaat 660
tttgtggagg gattgcagga attgcccgac gcgatcgccc agcagctcgg cgatgccgtc 720
aagctgcagt gggaagcggc tgagattgtc aaagaaggcg atcgctaccg caccactttc 780
caaaccccca gtggccccca aaccgtctcc tccaaagcca tcctgctcgc cgtccctgcc 840
taccgtgccg cccccctact caaatccctc gacactgccc ttgccgacga actcgctgcc 900
attccctatc cccacgttgg ggccgttacc ctggcctacc ccgccgatgc cctgcctcaa 960
cctttcgccg gattcggcca actcttcccg cgcggccaag gcatccgcac cctcggcacc 1020
atctggacct ccagcctctt ccccggccgc gccccggcag gctatcaatg caccctcagc 1080
tacatcggcg gtgccaccga ccctgacatt gctcaaatga cggacgaagc cttagctcga 1140
acggtccacc aagacctgag caaaactctg ttggtgaaag aagctgaacc gcgcgtcatg 1200
ggcgtacgcc gctggccccg cgccattcct caatacaccc tcggtcaccg acagcgcctt 1260
gcccgcattg acgaattgct cgcagactat tccggcttgg tgttgtgtac caattacttg 1320
gatggcgtgg cattgggcga ttgtgtcagg cgaggagagg ctcgggcagc ggatttggtg 1380
gaatggctgg cacaagctga gtag 1404
<210> 120
<211> 1455
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 41
<400> 120
atgacaaacc tagttgatag ccttattgtt ggtgctggta ttagtgggct gagtttagcc 60
tatagtctta accgtgaaaa aagtgtgcgc gaacctctaa aggttttggt tacagaaagc 120
cagaaccgag tcgggggaaa tattacaacc ggacgggctg atgatttttt atgggaagaa 180
ggccctaaca gttttgcccc cacccctgaa ttactcggac tagccgtaga tttagggctc 240
aaagaagaat tgatttttgc cgatcgcaaa ctaccccgtt atgtgtactg gaacctaatg 300
ttacatccag tacccatgaa cccccccgcc ctactctcct ccgaactcat cagcgccaga 360
ggtaaactcc gcgccgcatt aggagccata ggatttgtgc cacccccagt aggcgctcac 420
ctctctcaac agggaggaga agaaactatc acccagtttt tcgatcgcca tttaggttca 480
gaagtcctag aacgtctcgt ccagcccttc gtttcagggg tttatgctgg cgaccctcaa 540
cagttagcag tccgttccgc ttttagtcgc atagtagccg ctgaggaggc tggggggggg 600
ctactacctg gatttgtgcg atcgcgtctg aataaaaaag cccccgtctc tacccccgac 660
cccaacattc ccaaaactcg ccccggtgaa ttagggtcct tccgttatgg tctgcaaact 720
ctaccagaaa ccttagccag caaattaggc gatcgagtta agttaaattg gactatcgac 780
cgtttttatc ccaccgatca tcaaacctat attgctgaat tttccacccc agacggtcct 840
cagcaagtag aagcccgaac ccttgcctta atgacccccg cccatgttag cgctcgcctg 900
ttgcaacccc tacactctcc catcgctacc gcattaagcc aaattcccta tccccccgtc 960
gcttgtgtag tcctcgccta tcccaaatca gccttaaaac aacaactcaa aggctttggt 1020
aatttaattc cccgccgtca aggaatccgt acccttggca ctatttggac ctcaagttta 1080
tttcccggtc gcgccccaga atcttggcaa gtcctcagca attatattgg gggtgcaaca 1140
gacccggaaa ttggcgaaat ggatgatgat caaattgtcg ccgccgttca tcaagaccta 1200
cgccaaattc tgctggctga agatgtcccc cccaaggtct tagctgttca tctttggcgg 1260
cgcgctatcc cacaatatac tctcggtcat cagaaccgcc taaattgcat cgatgctgga 1320
ttgcgatcgy ttcccggact ttatctgtgt agcaactata ttgatggggt ttccgtcgga 1380
gactgtgtga ggcgcggtca gcaatgggca tctaaaattc agtcccatct tcatgactgc 1440
caaacagcca actaa 1455
<210> 121
<211> 1455
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 42
<400> 121
atgacaaacc tagttgatag ccttattgtt ggtgctggta ttagtgggct gagtttagcc 60
tatagtctta accgtgaaaa aagtgtgcgc gaacctctaa aggttttggt tacagaaagc 120
cagaaccgag tcgggggaaa tattacaacc ggacgggctg atgatttttt atgggaagaa 180
ggccctaaca gttttgcccc cacccctgaa ttactcggac tagccgtaga tttagggctc 240
aaagaagaat tgatttttgc cgatcgcaaa ctaccccgtt atgtgtactg gaacctaatg 300
ttacatccag tacccatgaa cccccccgcc ctactctcct ccgaactcat cagcgccaga 360
ggtaaactcc gcgccgcatt aggagccata ggatttgtgc cacccccagt aggcgctcac 420
ctctctcaac agggaggaga agaaactatc acccagtttt tcgatcgcca tttaggttca 480
gaagtcctag aacgtctcgt ccagcccttc gtttcagggg tttatgctgg cgaccctcaa 540
cagttagcag tccgttccgc ttttagtcgc atagtagccg ctgaggaggc tggggggggg 600
ctactacctg gatttgtgcg atcgcgtctg aataaaaaag cccccgtctc tacccccgac 660
cccaacattc ccaaaactcg ccccggtgaa ttagggtcct tccgttatgg tctgcaaact 720
ctaccagaaa ccttagccag caaattaggc gatcgagtta agttaaattg gactatcgac 780
cgtttttatc ccaccgatca tcaaacctat attgctgaat tttccacccc agacggtcct 840
cagcaagtag aagcccgaac ccttgcctta atgacccccg cccatgttag cgctcgcctg 900
ttgcaacccc tacactctcc catcgctacc gcattaagcc aaattcccta tccccccgtc 960
gcttgtgtag tcctcgccta tcccaaatca gccttaaaac aacaactcaa aggctttggt 1020
aatttaattc cccgccatca aggaatccgt acccttggca ctatttggac ctcaagttta 1080
tttcccggtc gcgccccaga atcttggcaa gtcctcagca attatattgg gggtgcaaca 1140
gacccggaaa ttggcgaaat ggatgatgat caaattgtcg ccgccgttca tcaagaccta 1200
cgccaaattc tgctggctga agatgtcccc cccaaggtct tagctgttca tctttggcgg 1260
cgcgctatcc cacaatatac tctcggtcat cagaaccgcc taaattgcat cgatgctgga 1320
ttgcgatcgc ttcccggact ttatctgtgt agtaactata ttgatggggt ttccgtcgga 1380
gactgtgtga ggcgcggcca gcaatgggca tctaaaattc agtcccacct tcatgactgc 1440
caaacagcca actaa 1455
<210> 122
<211> 1452
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 43
<400> 122
atgacaaacc tagttgatag ccttatagtt ggtgctggta ttagcgggct aagtttagcc 60
catagcctga accgtgaaaa aaacccccgc tcacccctaa aagttttggt cacagaaagc 120
cagaaccgag tcgggggaaa tattacgacc ggacgcgcat ctgatttttt atgggaagaa 180
ggccctaaca gttttgcccc cacccctgaa ttactcggac tagccgtaga tttggggctt 240
aaacaagaat tgatttttgc cgatcgcaaa ctaccccgtt atgtctactg gaaccataag 300
ttacatccag tacccatgac cccccccgcc ctactctcct cccaactaat cagccccaga 360
ggtaaactcc gcgccgcctt gggagccata ggatttgtgc cacccccagt aggcgctcac 420
ctctctcaac agcgaggaga agaaactatt acccagtttt tccatcgcca tttaggttca 480
gaagtcctag aacgtctcgt ccagcccttc gtttctgggg tttatgctgg cgaccctcaa 540
cagttagcag tgcgttccgc ctttagtcga ttagtagccg ccgaagacgc agggggtgcg 600
ctactacctg gatttgtgcg atcgcgcctt aataaaaaag ccaccaaaga caccaccgcc 660
gaccccaata ttcccaaaac tcgccccggt gaattagggt ccttccgtta tggcctggaa 720
actctgcccg aaaccttggc cagtaaatta ggcgatcgag ttaagttaaa ttggaccctc 780
gaccgttttt atcccaccga tcatcaaacc tatattgctg aattttccac cccagacggc 840
ccccagcaag tagaaacccg aaccctcgct ttaatgaccc ccgcccatgt tagcgctcgc 900
ctcttgcaac ccctacactc tcaaatcgct tccgcattaa gccaaattcc ttatcccccc 960
gtcgcttgtg tcgtactcgc ctatcctaaa tcagccttaa aacaacaact caaaggcttt 1020
ggtaatttaa ttccccgcca tcaaggaatc cgtaccctcg gcactatttg gacctccagt 1080
ttattccccg gtcgcgcccc agaatcttgg caagtcctca gcaattatat tgggggtgct 1140
acagaccctg aaattggcga aatggatgat gatcaaattg tcgccgccgt tcatcaagac 1200
ctacgccaaa ttctgctggc tgaagatgtc ccccccaaag tcttggctgt ccatctctgg 1260
cggcgcgcta tcccacaata tactctcggt catcaagatc gcctaaattc catcaatgct 1320
ggattgcgat cgcttcccgg actttatctg tgtagcaact atattgatgg ggtttccgtc 1380
ggtgactgtg tgaggcgcgg tcagcaatgg gcatcgcaaa ttcagtccca ccttcatcca 1440
acagccaact aa 1452
<210> 123
<211> 1437
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 44
<400> 123
atgaccatca cgccaccctc tgagaacaat caacccatag atgtccttgt cgttggggca 60
ggaatctcag gattaacgat cgcccatgaa ctagcgatct ctaaaaaata tagcgtcttg 120
gtagcagaag cacaggatcg tgtgggtgga gccataacca gtgccaaaaa tgacgaaggt 180
tatcaatggg aagagggccc aaatagtttc caacccgctc ctgaactatt gcgtctagcg 240
gtacaggtag gactcaagga tgaattggtg ctagctgatg gcaaattacc ccgtttcgtt 300
tttctaaatg ggaagttaaa cgccttaccg atgactccag cgagtgcgat cgcatctaaa 360
atattgactt ggggcggcaa gattcgtcta gctctaggtg caatcggctt tgcgcgtcca 420
gccatggctg gagaagaatc cgttgatcaa ttcttttcac gtttgctagg cagacaagca 480
gttgaaagat tagttgcacc gtttatctca ggtgtatacg caggcgatcc caagcgactc 540
agtgccaagg ctgccttttc taagattgct cgtctagaaa cctatggtgg gttaatcgca 600
ggcgcaatat tatctagcaa acaacggaaa gccgaaaaga ttaatgatcc gaatattccc 660
aaaactaaag ctggtgaact aggttcattt cgtcaaggta taaaaatgct gcctgaagcg 720
atcgctacca agttaagaga gcaaggttca gccatcaaac aacaatggac tttgcgatcg 780
ctggaaaaac aaggcgaagt ctatatttct aagttcgata cacccacggg tgaagaaact 840
gtgacatcgc gatcgattgt tctcgctacc ccagcctatg tcacggcaaa attactgcaa 900
gattacttgc cagccgccag tcaagccttg aatgagattt tctatccgac cgttgcttgt 960
gtggtacttg cttatcctaa gagcgaattt gcctatgata tgaagggctt tggcaatctg 1020
attccccgca ctcaaggagt aagaacttta ggcactatct ggtcatcgag cctatttact 1080
ggacgtgctc cagaaggttg gcaactatta ctaaacttta ttggcggcac gcttgatccc 1140
gctctagcca aactttcgga accagaaatc attgccgctg tgcatcaaga tttaaagaaa 1200
acaattcttc gacctgacac gaaagccgaa ccaaaagcga tcgcagttca tgtatgggat 1260
aaagcgatcc cccaatacga aatcggacat ttagaaaggc ttgccattgt ggaagcagaa 1320
ttacagaaat ctcaaggact ttatgtcagt gccaatttta tcggaggtgt ggctctaggt 1380
gactgcatca agcgtagttt gcaagaagcc aataaaattg atgcatatct aaagtaa 1437
<210> 124
<211> 1437
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 45
<400> 124
atgacctccg cccagattcc tgaagttaat ccattagatg ttcttgtcgt tggggcaggg 60
atttcaggtt taacgatcgc ccatgaatta gcgatcgcta aaaactatcg cgtcttggtg 120
gcggagacac aggatcgggt gggcggcgca attactagcg ccaaaaatga cgatggctat 180
caatgggagg aaggacctaa tagcttccag ccagcccccg aattattacg tttagccgta 240
gaagtcggac tcaaggatga attggtgctt gcggatggca aactaccccg ttttgtattt 300
ctcaacggca agttaaacgc tttgcccatg tctccgccaa cggcgatcgc ttcccgaatt 360
ttgacttggg gaggcaaaat ccgtctcgct ttaggggcaa tgggttttgc acgtcctgca 420
atggcgggag aagaatccgt cgatcgcttc ttttcgcgcc tgttaggcag acaagcggtc 480
gatcgcttgg ttgccccctt catttctgga gtatatgcgg gcgatcccaa gcggctcagt 540
gccaaggctg cctttgccaa aatcgccaag ctggaaacct atggcggttt gcttgctggg 600
gcaatattat cgagtaaaga gcgcaaacaa aaacttaacg atcctcgcat tccgaaaacg 660
aaggcagggg agttaggttc ctttcgcgag ggcataaaga tgctccctga agcgatcgcc 720
gctaaattaa gggcacaggg tacagccgtt aaacaacaat ggactctgcg atcgctagag 780
aagcaaggcg gaatctatat ttccaaattt gataccccaa cgggcgaaga aattgtgacc 840
tcgcgatcgg tcgttctttc cacccctgcc tatgtcactg caaaattatt acaagattat 900
ttacccgccg ccagccaagc cttaaatgag attttttatc cgacggtagc ctgtgtcgtg 960
cttgcctatc ccaaaagcga atttctctat gacatgaaag gctttggcaa tttgattcct 1020
cggacagagg gagttagaac cctagggaca atttggtcat ctagcctctt ttcaggacgc 1080
gcccccgcag gttggcaact attgctcaat tttatcggtg gcaccctcga tcccgcacta 1140
gcccatttat ctgaagccga aatcattgct gccgtgcatc aagatctcaa aaaaacgatt 1200
cttcgccccg atacgaaggt atctcccaaa gcgatcgccg ttcatgtgtg ggataaggct 1260
attcctcaat acgaaatcgg gcatttagag cgccttgcca cggtggaagc cgaattgcaa 1320
aaatcctcag ggctatatgt cagtgctaat tttattggcg gagtcgcctt aggtgactgc 1380
atcaagcgca gcttgcaaga agctcataaa attgcggctt ttttagaaac catttag 1437
<210> 125
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 46
<400> 125
atgacttctg ctcaaaccac cgaagttaat caacccttag atgttcttgt tgttggggca 60
ggaatttctg gtttagcgat cgcccatgaa ctcgcgattg ccaaaaatta ccgtgtttta 120
gtagcagaag ctcaagatcg cgttggtggt gcaattacca gtaaccgtaa tgatgacggc 180
tatctctggg aagaggggcc caatagcttt cagcctgcac cagagttatt gcgtctagcc 240
gtagaggtag ggctcaagga tgaattggta cttgccgatg gtaagttgcc gcgatttgta 300
tttctcaatg gcaagttaaa tgcgttgccg atgagtccac ctaccgcgat cgcctccaaa 360
atcctgactt ggggcggcaa gattcgccta gctctgggtg cattaggttt tgcgcgtcct 420
gccatgagtg gtgaggaatc tgttgaccaa tttttttcgc gcctcttagg taagcaagca 480
gtggagcgct tagttgcccc ttttatctct ggggtctatg cgggtgatcc aaagcgtctc 540
agtgctagag ctgcattttc taaaattttc cgtctcgaga atggctatgg tggtttgctc 600
acaggggcaa tcctgacggc aaaagatcgc aaagcccaaa agctgaacga tccgaatatt 660
cccaaggtga agtctggtga actaggttct ttccgacaag gcattaaaat gctgcccgaa 720
gcgatcgcaa ccaaactcag agatcaaggt acggctgtca aacaacaatg gactttgcga 780
tcgctagaaa agcaaggcga aatctatgtt tctcagtttg gtacgcctac tggtacagaa 840
acaattactt cgcgatctgt ggtattgact accccagcct atgtgagcgc caagttattg 900
cagggctatt tacctgctgc aagccaagcc ctgagcgaga ttttctatcc gaccgtggca 960
tgtgtcgtac ttgcctatcc caaaagcgaa tttgcttatg acatgaaggg attcggtaat 1020
ctcattcccc gcacgcaagg tgtaagaact cttggaacaa tttggtcatc cagtctattt 1080
gcagggcggg ctcctgatgg ttggcaatta ttgctgaact ttataggtgg tactcttgat 1140
ccagatttag caaatctttc ggaagcgaag atcgttcaag cagtacatca agatctcaag 1200
aaaaccctgc tacgtcctga tagcaaagtc gaacccaagg tgatcgctgt acatgtgtgg 1260
gacaaggcaa ttcctcagta tgaaatcgga catttagagc gtcttgccac tatcgaaact 1320
gaattgcaaa agtcacaggg tttgtatgtc agcgcgaatt ttattggtgg tgttgcgctt 1380
ggtgattgca ttaagcgtag cctccaagaa gcagataaaa ttgcagcatt tttgaagtaa 1440
1440
<210> 126
<211> 1536
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 47
<400> 126
atgaactctt ctgttgagca ctttagcggg acgcagtcct tgcaggccga ggtggtggtg 60
gtgggggcgg gcatcagcgg cctaacgttg gccttgcgat tacaacaggg cttatccccc 120
aaggatgaat ccacccagcc gctgctcctg gccgaggcca gctcccgcgt ggggggctgt 180
atctccaccc aatccaaaga cagctatcgc tgggaagagg gccccaacag ctttaccccc 240
gtccccgccc tgcttaacct cattgccgag gtgggcttgg cggagcacct ggtgctggcc 300
gatgccaagc tgccccgcta catctactgg gaaaaagagc tgctgccggt gcccctgagc 360
ccttctgcag ccattggctc gcggctgttg agcgtgggcg gcaagttgcg ggccttacgt 420
gggttgttgg ggtttgtcgc gccgccgcca ggcggagagg aaacggtgcg gcaattcttc 480
cggcggcaac tgggatccga ggtggtggaa cggctggtcg aacctttcac ctcaggggtg 540
tatgccgggg atccggatca gctcagcgcc ttggcagcct tcccccgcat tgccggcttg 600
gaagaacgct acggcagcct ctttgccggt gctgtgcagg ctttgcgcag ccgttatcgc 660
tacgcgacgc tcccgcgaac ccgtcaccag gacagcgcca actcccccat ccagcctcct 720
cccaagcggg gccaactggg taacctgcgg caggggttgc agcagctacc tgaggcgatt 780
gcccagaaac tgggcagtgc gctgcggctg ggctggcggg ctgtgcatct caagcgggat 840
gagacgggct accgggtggg cttcgtcata cacgacagcg gcgcggagca cacggcccca 900
gaagaaatcc actgggtggc ggctcaacag gtggtgctca ccctccctgc ctacgctgct 960
gccaccctcc tgcaggatct caacccccag gccagccggc tgctgaggga aatcccctac 1020
ccgcctgtgg cggtggtggc tttggcctat ccagaagaag ccctccccca accgctgcga 1080
gggtttggcc atctgatccc tcgctctcag ggcctgcgca ccctgggcac catctgggcc 1140
tcctccctct tcccagagcg ggctcctcag ggctatcact gcctgatcag cttcatcggg 1200
ggagctaccg atgcggcttt tgcccgccag aaagggatcc cgcccattac ggcgctctcc 1260
cctgacgagc gggcccaaat tgtgcatgca gaactcagcc agatcctgct cacccgccct 1320
gtcgaaccca tccgcctggg agagcgcctg tggccccagg ctatcccgca atatacgctt 1380
ggccaccggc agcggattgc ccaattgcag gccagcctgg ccgaccaaac cccaggggtg 1440
tgggtgtgtg ccaactacct ggatggcgtg gccttgggag attgtgtgcg gcgggccgag 1500
gccctagccc agcagatcct gtccgtccgg cgttag 1536
<210> 127
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 48
<400> 127
atgacctccg cccagattcc tgaagttaat ccattagatg ttcttgtcgt tggggcaggg 60
atttcaggtt taacgatcgc ccacgaacta gcgatcgcta aaaactatcg cgtcttggtg 120
gcggaggcac aggatcgcgt gggcggcgcg attactagcg caaaaaatga cgagggctat 180
caatgggagg aaggtcccaa tagtttccag ccagcccccg aattattacg tttagccgta 240
gaagtcggac tcaaggatga attggtgctt gcggatggca aactaccccg ctttgtattt 300
ctcaacggca agttaaacgc tttgcccatg tctccgccaa cggcgatcgc ctcccaaatt 360
ttgacttggg gtggcaaaat ccgtctcgct ttgggggcaa tgggttttgc acgccctgcg 420
atggcgggag aagaatccgt tgatcgcttc ttttcgcgcc tgttaggcag acaagcggtt 480
gatcgcttgg ttgctccctt catttctgga gtatatgcgg gcgatcccaa gcggctcagt 540
gcgaaggctg cttttgcaaa aattgccaag ctggaaacct atggtggttt gcttgctggg 600
gcaatattat caagtaaaga gcgcaaagcc caaaaactta acgatcctcg cattccgaaa 660
acgaaggctg gggagttggg ttcctttcgc gagggaataa aaatgctccc tgaagcgatc 720
accgctaaat taagggcgca gggtacagcc gtcaaacaac aatggactct gcaatcgcta 780
gagaagcaag gcgaaatcta tgtttccaaa ttcgctaccc ccacgggcga agaaattgtg 840
acctcgcgat cggtcgttct ttccaccccc gcctatgtca ctgcaaaatt attacaagat 900
tatttgcccg ccgctagcca agccttaaat gagatttttt atccaactgt tgcctgtgtg 960
gtacttgcct accccaaaag cgaatttcgc tatgacatga aaggctttgg caatttgatt 1020
cctcggacag agggagttag aaccctagga acaatttggt catctagtct ctttgcagga 1080
cgcgcccccg caggttggca actattgctc aattttatcg gcggcacgct tgatcccgca 1140
ctagcccatt tatcagaagc agaaattatt gctgccgtgc atcaagatct caaaaaaaca 1200
attcttcgcc ccgatacgaa ggtatctccc aaagcgatcg ccgttcatgt gtgggataag 1260
gctattcctc aatacgaaat cgggcattta gagcgacttg ccacggtaga agctgaattg 1320
caaaaatctt cagggctata tatcagtgct aattttattg gcggagtcgc cttaggtgac 1380
tgcatcaagc gtagcttgca agaagctcat aaaattgcgg cttttttaga aaccatttag 1440
1440
<210> 128
<211> 1437
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 49
<400> 128
atgaccctca cgccatcttc tgaagaaaat cagccattag ataccctaat cattggggca 60
ggaatctcag gattaacgat cgctcatgag ctagtgatcg ctaaaaacta ccgcattttg 120
gttgcggagg cacaggatcg cgtgggtggt gccataacta gcgccaaaaa tgacgaaggc 180
tatctatggg aagagggccc aaatagtttt caacccgcac ctgagctatt gcgcttagcg 240
gtacaagtgg gactcaagga tgaattggtt ctagccgatg gcaaattgcc ccgttttgtc 300
tttttgaatg gcaagttaaa tgccttgcca atgagtccac cgaccgcgat tacttccaaa 360
atcctgactt ggggcggcaa gattcgtctg gctttggggg cgatcggctt tgctcgtcca 420
gcgatggctg gggaagaatc tgtcgatcaa ttcttttcac gtatattagg caagcaagca 480
gttgaaagat tggttgcgcc gtttatatct ggtgtgtatg caggtgatcc caagcgactc 540
agtgccaagg ctgctttttc aaagattgcc aaactagaaa cctatggagg cttgctctca 600
ggagcaatat tatccagcaa agaacgtaaa gcccaaaagc tcaacgaacc gaatattccc 660
aaaactaaag ctggtgaatt aggatcgttt cgacaaggca tccagatgct gccagaagcg 720
atcgcgtcaa agttaaggga gacaggtaca gcagtcaagc aaaaatggac tttgagatcg 780
ctagaaaaac aaggcgatat ctatatttct aagttcgata caccctcagg tgaagaaact 840
gtgacatcgc gatcggtggt tttgactacc cccgcctatg tcaccgccaa acttttggaa 900
gattatttgc cagccgctag ccaagctctg aatgagattt tctatcctac tgttgcttgc 960
gtggtgcttg cctatccaaa gagcgaattt gcccatgaca tgaagggctt tggcaacctg 1020
attccccgca ctcaaggagt gagaacgtta ggcacaatct ggtcatcaag tttatttgct 1080
ggacgtgccc ctgaaggttg gcaactatta ctaaacttta tcggcggcac tctcgatcct 1140
gccctagcca agctttcaga atcggaaatt attgctgctg ttcatgcaga tctaaagaaa 1200
acaattctcc gacctgatac taaagccgaa ccgaaggcga tcgcagttca tgtatgggat 1260
aaggcaattc cacaatacga aatcggacat ttagatcgac ttgccacagt ggaagcagaa 1320
ttacagaaat cacaaggact gtatgtaagc gcaaacttta tcggaggtgt ggctcttggt 1380
gattgcatca agcggagctt gcaagaagcc aataaaatcg atgcattttt aaaatga 1437
<210> 129
<211> 1509
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 50
<400> 129
atgtccaacc ataatcatgc tggctcaccg atcgacctgt tggtagttgg cgctgggatc 60
agtggcctca ccgtggctca tgacctggcc agcaattctg tcgctgaggc taatgggtca 120
aaccagctca ggattatcgt caccgaggca caaaatcggg tgggcggggc gatcgtctcg 180
cagcgggatg aagtaggttt tcaatgggaa gaagggccca atagctttca gccagcccca 240
gagctactca aactggcggc ggcggtgggg ctaaaggatg aattagtatt tgcggatggc 300
aagttgccca ggtttgtgtt ctggaatggg aagttaaatg ctctgcccat gtcaccgcag 360
ctgctaacca aatttaattt attaacgatc aagggcaagc taagggcttt cctgggggcg 420
atcggctttg tgcgaccagc ggcagcgggg gaagaaactg tggcgcagtt ttttaagcgg 480
catctggggc aagaggtggt cgatcggctg gtagtaccgt tcatctctgg ggtttatgca 540
ggcaatacgg acaagctgag tgcggcggcg gcatttggca agattttccg gttagaaaaa 600
aactacaatg gtctggtggc gggggcgatc ctgtcgcggt tggccaagcg caagcaagag 660
aaggcgcaaa ccaaccagcc agcaaatatt tacgatcgcc aggagatccc caaaaccaag 720
ccaggacagt tgggatcatt taaaaatggg attgaggctc tgcccagggc gatcgccgaa 780
gatttaatcg acaaaggtca tgaggtgcga ttgcaatggc ggttggacaa aatccagccc 840
aactctgatg ggacatatag cgccacctat gagacacccc acgggatcga aacgatcacg 900
gcgcgggctt tgctccttac tacgcctgcc tatgtttcca gtatgctgct gcaagatatt 960
gcccctgatg cagctcaatc tttgggtgag atttattacc cgccagtggc ctgtgtggtg 1020
ttggggtatc cggatgcggc gatgaaacgg gatatgaatg ggtttggtaa tttgatcccg 1080
cgtagtcagg gcatccgtac gttgggcacg atctggggat cgagtttgtt tagcgatcgc 1140
gctccggctg gctatcatct gctgctgaat tttattggtg ggtcgttgga tactggcatt 1200
gccgatctat ctgaacctga aatagctcag gcggtgcata gtgacctgaa gcaaacgttg 1260
ctgaaaccag acaccacgat cgaaccgaag gtgttggcgg tgcatttgtg gcaacgggcg 1320
atcccgcagt atgaggtcgg tcacctcgat cgattggcgc gggttgaaag ggatctggct 1380
aatcatcctg ggttgtttgt gagtgctaat tttattggtg gcgtggcgct gggggattgt 1440
gtgaagagga gttttggtac ggctgaacaa attaaggggt ttttaaatct gtctactaaa 1500
aaaaaataa 1509
<210> 130
<211> 1440
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 51
<400> 130
atgtccctaa caccttcctc tgcaaccaat caacccttag atgttcttgt cgttggcgca 60
gggatatcag gcttaacgat cgcccatgaa ctggcgatcg ccaaaaaata tcgcgtcttg 120
gttgcggaag cacaggatcg ggtgggcggt gcgatcacca gcgctaaaaa tgatgaaggt 180
tatcaatggg aagagggtcc aaatagtttt cagcctgcgc ctgagctatt gcgtttagca 240
gtacaggtgg gactaaagga cgaattggta ttagctgatg gcaaattgcc ccgttttgtc 300
tttctcaatg gcaagttaaa cgctttaccc atgagtccac cgacagcgat cgcctccaaa 360
attttgactt ggggaggcaa aattcgtctg gctttaggtg cgatcggctt tgcgcgtccc 420
gcaatggctg gcgaagaatc cgttgatcaa ttcttttcgc gccttctagg taaacaagcg 480
gttgaaagat tagttgcacc gtttatctca ggtgtatacg caggtgatcc taaacgactc 540
agtgccaagg cagcctttgc caaaatcttt cggttagaaa atagctataa cggtttgctc 600
gcaggcgcga ttctttcggc aaaggaacgt aaagcccaaa agctgagtga tcccaatatt 660
cctaaagtta aagctggtga attgggttca tttcggcaag ggattaagat gttgcctgag 720
gcgatcgcca cgaaattgcg agatcaaggt acagccgtca agcagcaatg gactttgcga 780
tcgctagaaa agcaagacga aatctatatt tctaagtttg atacgcctac gggtgaagaa 840
acggtgcgat cgcgatcggt agtactcagt acccccgcct atgtcactgc taagctattg 900
caagactatc taccagcagc tagccaagcc ttaaacgaga ttttttatcc gactgtagcc 960
tgtgtagtga tggcatatcc taagagcgaa tttgcctatg acatgaaagg atttggcaat 1020
ctcattcccc gcactcaagg cgtgagaact ctaggcacaa tctggtcatc cagtctattt 1080
gcaggacgcg cccctgaagg ttggcaactg ttattaaact ttatcggcgg cacgctcgat 1140
cctgctttag ctaagctatc agaacctgaa atcattgctg ccgttcatca agatctcaag 1200
aaaaccatcc tgcgtcctga tacaaaagcc gaaccgaagg cgatcgcagt ccatgtttgg 1260
gataaagcca ttcctcaata cgagattgga catttagacc gccttgcgac ggtagaaaaa 1320
gaattacaaa aatctcaagg cttgtatatc agtgccaact ttattggcgg tgtggcttta 1380
ggtgattgca tcaagcggag tttgcaagaa gctactaaaa ttgatgcatt cttaaaataa 1440
1440
<210> 131
<211> 1452
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 52
<400> 131
atgaccacct ctcaagaaac taaagctagt caaccgttag atgttcttgt cgttggggca 60
ggaatatcgg gtttaacgat cgcccacgaa ctggcgatcg ctaagcacag acatgtctta 120
gttgctgaag cacaggatcg agtgggtggt gcgattacca cggctagcaa taacgagggt 180
tatctttggg aagaagggcc caacagtttt caacctgcac ccgaactatt gcgcctagcg 240
gtagaggttg ggcttaaaga tgaattggta ctggcagatg gcaaattacc aagattcgtg 300
tttctcaatg gcaagttaaa tgctctacca atgagtccac cgacggcgat cgcctccaag 360
atcttgagtt ggggaggcaa aatccgtcta gctttaggtg ctcttggctt tgcgcgtcca 420
gcgatggctg gcgaagaatc tgtcgatcga ttcttttcgc gtttattggg gaaacaggct 480
gttgaaagat tggttgcccc ctttatctct ggtgtctatg caggcgatcc gaagcggctc 540
agtgcgaagg cagccttttc caaaattgcc aaactcgaaa cctatggagg cttgctcgca 600
ggagcaatat tgtcgagcaa agagcggaaa gcccaaaagc tcaatgaccc taatattccc 660
aaaactaagg ctggtgaact tggctctttt cgccaaggga ttaaaatgct acccgaagcg 720
atcgctgcta agttaagagc gcaagggaca ccagttaagc agcaatggac gttgcgatcg 780
ctccagaagc aagatgaaat ttatatcgct aagttcgata cgccaacggg tgaagaggtc 840
gtcacatcga agtctgtggt tctcagtaca ccagcctatg tttccgccaa actactgcaa 900
gattatttgc ctgctgccag tcaagcgttg agcgagattt tttatcccac tgttgcttgc 960
gtggtgcttg cttatcctaa gagtgcattt gcctatgaca tgaaaggctt tggcaacttg 1020
attcctcgca ctcaaggcgt gagaactctc ggcacaattt ggtcatcaag tctatttgct 1080
ggacgcgctc ccgatggctg gcaactgtta cttaacttta tcggcggtac gctcgatcct 1140
gctctagctc aactttcgga atctgaaatc attcaagccg tacatcaaga tctcaagaaa 1200
acaattctcc gtcctgatac gcaagtggaa ccaaagacga tcgccgttca tgtatgggat 1260
aaggcaattc cacagtacga gatcggtcat ttacaacgcc ttgcaaccat caaagcagaa 1320
ttacaaaaat ctcaaggatt gtacatcagc gctaatttta tcggcggtgt agctctaggc 1380
gattgcataa agcgtagtct gcaagaatcg atcgaaattg atgagtttct atcgagaaca 1440
aactctcttt aa 1452
<210> 132
<211> 1446
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 53
<400> 132
atgaaccaat tagccgatcg cccatcaggt gagcaaactt tggatgtgct tgtggtggga 60
gcaggaattt caggcttgac gatcgcctat gatttggcaa ttaatcagca tcggcaggtt 120
ttagtcgctg aagcgcaaga tcgcgttggt ggggcaatcg tctctaaaca aaatgacgaa 180
ggctatcttt gggaagaagg acctaatagc tttcagccca cacccgaact gctgcgccta 240
gcagtcaatg tgggacttga ggatcaattg gtgctagcca atggcaagtt accgcgcttt 300
gtatttttaa agggcaaact aaacgctttg cctatgagtc cccccgccgc gatcgccaca 360
ccattattgg attggggtag caagattcgc ttggcactag gggcgatcgg ttttgcccgt 420
cccgccatgg ctggcgaaga gtcggtggat cagttcttta gcagactgtt gggcaagcaa 480
gcggtggcgc gtttggtagc accgtttatt tcaggagtat atgcaggtga tcccaagcga 540
ctaagtgcta gggctgcttt tgccaaaata tttcgcctcg aaaataatta tggcggctta 600
gtggcaggtg caattttatc aggtaaagat cgccaagctc aaaaggctaa aaatgccgat 660
ctgcctaagg tcaaggcagg tgaactaggg tcatttaaac aggggattaa gatgttgcca 720
gaggcgatcg ccactaagct gcgatcgcaa ggcacagcag ttaaacagca atggactttg 780
cgatcgctag atcggcaagg tgatcattac atcgccaagt ttgacacccc cacgggcgaa 840
gaaacggtca tgtcccgtgc tgtagtactg gctacccccg cctatgtgac agccaactta 900
ttaaaagact atttgccatc ggcaagccaa gccctccgca aaatctttta tccaaccgtt 960
gcctgtgtgg tgctggctta ccctaaaacc gaatttgcct atgatatgca gggctttggc 1020
aacctcatcc cgcgtacgga aggagtccgc accctcggta cgatctggtc atcgagttta 1080
tttgcaggac gcgcccccca aggttggcaa ctactgctca acttcatcgg cggcacctta 1140
gatccagccc tagcacagct ttctgaggca gaaattatcc aagctgtgca tcaggatttg 1200
aaaaaaacta tccttcgtcc tgataccaaa gcagaaccca aggcgatcgc tgtgcatcga 1260
tgggacaagg cgattccgca gtatgaaatt gggcatttac aacttctcgc caccgtggaa 1320
gcggagttac aaaagtctca aggtttatac atcagcgcca attttattgg tggagttgcc 1380
ctcggtgact gcatcaagcg cagcttgcag gaagctatta aaattgagca atttttagcc 1440
aaataa 1446
<210> 133
<211> 1467
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 54
<400> 133
atgccctcac cccaatctgc tacgaaccaa tcagatgatc gcccatcagg tgggcaacct 60
atagatgtgc tggtagtggg cgcaggagtt tcaggtctga cgatcgccca tgatttggca 120
attaattacc agcgccaagt tttggtggcg gaggcgcaag atcgcgtggg cggagcgatt 180
atctcaagac aaaataatga aggttatctc tgggaagaag gtcccaatag ctttcagcct 240
gctcccgaac ttttgcgtct agcggtcaat gtcggacttg aggatcaatt agtgctagcc 300
aatggcaagt tgccgcgctt tgtatttttg aagggcaaat taaacgccat ccccatgagt 360
ccccccgccg cgatcgccac accattgttg gattggggta gcaaaattcg cttggcgctg 420
ggggcgatcg gctttgcccg tcccgcgatg gcgggtgagg agtcggtgga tcaatttttt 480
agcaggctat tgggtaagca agccgtagcg catctagttg cgccatttat ctcaggcgta 540
tatgcaggtg atccgaagcg attgagtgct agggctgcct ttgccaaaat atttcgcctt 600
gaaaataact atggcggtct agtggcaggc gcaattttat caggtaagga tcgccaagct 660
cagaaagcca aaaatacgga tctgcctcag gttaaggcag gtgagctagg gtcatttaag 720
caaggaatca agatgttgcc agaggcgatc gctacgaagc tgcgatcgca aggcacgccc 780
gtcaaacaac aatggactct gcgatcgcta aatcgacaag acgatattta cattgccaaa 840
tttgacacgc ccactggcga agaaatagtt atgtcccgcg ccatggtaat ggctacacct 900
gcctatgtga ctgccgactt attaaaagac tatttaccat cggcaagcca agccctcagc 960
aaaatctttt atccaaccgt tgcctgtgtg gtgctggctt accctaaaac tgaatttgcc 1020
tatgatatgc agggctttgg taatttgatc ccgcgtacgg aaggagtccg cacccttggt 1080
actatctggt catcaagttt atttgcagga cgcgctcccc aaggttggca actactactc 1140
aacttcatcg gtggcacatt agatccagct ctagccaagt tatccgactc agagatcatc 1200
caagctgtgc atcaggattt aaaaaaaact atccttcgtc ctgatactaa agcggaaccc 1260
aaggcgatcg cagtgcatcg atgggataag gcaattccgc agtatgaaat cggacattta 1320
caactcctcg ccactgtgga agctgaattg aaaaagtctc aaggtctata cattagcgcc 1380
aattttatcg gcggcgttgc tctcggtgac tgcatcaagc gcagcttgca agaagctatt 1440
aaaattgagc aatttttagc caagtaa 1467
<210> 134
<211> 1692
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 55
<400> 134
atgatgttga cccagactcc tgggaccgcc acggcttcta gccggcggtc gcagatccgc 60
tcggctgcgc acgtctccgc caaggtcgcg cctcggccca cgccattctc ggtcgcgagc 120
cccgcgaccg ctgcgagccc cgcgaccgcg gcggcccgcc gcacactcca ccgcactgct 180
gcggcggcca ctggtgctcc cacggcgtcc ggagccggcg tcgccaagac gctcgacaat 240
gtgtatgacg tgatcgtggt cggtggaggt ctctcgggcc tggtgaccgg ccaggccctg 300
gcggctcagc acaaaattca gaacttcctt gttacggagg ctcgcgagcg cgtcggcggc 360
aacattacgt ccatgtcggg cgatggctac gtgtgggagg agggcccgaa cagcttccag 420
cccaacgata gcatgctgca gattgcggtg gactctggct gcgagaagga ccttgtgttc 480
ggtgacccca cggctccccg cttcgtgtgg tgggagggca agctgcgccc cgtgccctcg 540
ggcctggacg ccttcacctt cgacctcatg tccatccccg gcaagatccg cgccgggctg 600
ggcgccatcg gcctcatcaa cggagccatg ccctccttcg aggagagtgt ggagcagttc 660
atccgccgca acctgggcga tgaggtgttc ttccgcctga tcgagccctt ctgctccggc 720
gtgtacgcgg gcgacccctc caagctgtcc atgaaggcgg ccttcaacag gatctggatt 780
ctggagaaga acggcggcag cctggtggga ggtgccatca agctgttcca ggaacgccag 840
tccaacccgg ccccgccgcg ggacccgcgc ctgccgccca agcccaaggg ccagacggtg 900
ggctcgttcc gcaagggcct gaagatgctg ccggacgcca ttgagcgcaa catccccgac 960
aagatccgcg tgaactggaa gctggtgtct ctgggccgcg aggcggacgg gcggtacggg 1020
ctggtgtacg acacgcccga gggccgtgtc aaggtgtttg cccgcgccgt ggctctgacc 1080
gcgcccagct acgtggtggc ggacctggtc aaggagcagg cgcccgccgc cgccgaggcc 1140
ctgggctcct tcgactaccc gccggtgggc gccgtgacgc tgtcgtaccc gctgagcgcc 1200
gtgcgggagg agcgcaaggc ctcggacggg tccgtgccgg gcttcggtca gctgcacccg 1260
cgcacgcagg gcatcaccac tctgggcacc atctacagct ccagcctgtt ccccggccgc 1320
gcgcccgagg gccacatgct gctgctcaac tacatcggcg gcaccaccaa ccgcggcatc 1380
gtcaaccaga ccaccgagca gctggtggag caggtggaca aggacctgcg caacatggtc 1440
atcaagcccg acgcgcccaa gccccgtgtg gtgggcgtgc gcgtgtggcc gcgcgccatc 1500
ccgcagttca acctgggcca cctggagcag ctggacaagg cgcgcaaggc gctggacgcg 1560
gcggggctgc agggcgtgca cctggggggc aactacgtca gcggtgtggc cctgggcaag 1620
gtggtggagc acggctacga gtccgcagcc aacctggcca agagcgtgtc caaggccgca 1680
gtcaaggcct aa 1692
<210> 135
<211> 1698
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 56
<400> 135
atgctgacta gccatgccac taacatctac gcgaactcaa aggccaggct tcagatccgc 60
gagatcgctc ggccgacggt gaccggtgtc tcgaggtcag gcgttcttca ggatgtcgcc 120
tctccgctcc cacggcagat tccgaggcca gctcaacgtg ctgcgcgtcc tacttctgct 180
acggccactt caagtggggt acccacggcg gacagtgccc gtggcacgaa gatcgttgac 240
aatgtgtatg acgtgattgt ggttggcggt ggcctctccg ggctcgttac gggtcaagcc 300
ctctcggcgc aacatggtgt taataacttc ctcgttaccg aggctcgcga gcgcgtcggc 360
ggcaacatta cgtccatgtc gggcgatggc tacgtgtggg aggaaggccc aaacagcttc 420
cagccaaatg atagtatgtt acaagtagct gtggatgccg gggtggagaa ggacctggtg 480
ttgggagacc ccaaggcacc ccgatttgtg tactggcaga acaagctccg tcccgtcccc 540
agcggccctg atgcgctcac ctttgacctc atgtcccttc cggggaagat ccgagctggt 600
ctcggagcca ttggcctgat caacggagcc atgcctaact ttgaggagag cgtggagcag 660
ttcatccgtc ggaacctggg tgacgaggtg ttcgagcgcc tcattgagcc gttctgctcg 720
ggggtgtacg cgggagatcc atccaagcta tccatgaagg cagcgtttaa caggatctgg 780
attttggaga aggacggcag cagtctggtg gggggtgcgt tgaagctgtt tcaggagcgc 840
cgcaagaacc cgccgccacc tcgcgatccc cgcctgccgc ctaaacccaa gggccaaacg 900
gtgggttctt tccgtctcgg tctcaagatg ttgcccgagg cgattgagcg gcgtatcaag 960
gaccaagtca gggtcaactg gaagctggtg tcgttgacgc gcgacggcga tcgctacagc 1020
ctggtgtacg acacgccgga gggccgagtg caatgctaca gccgtgcggt ggcgctgacg 1080
gcaccgtcgt atgttgtggc ggatcttatc aaggctgagg tgccagccgc tgcggaggct 1140
ctgtcctcgt tcgactaccc cccggtgggg gccgtcactc tgagctaccc cctgtcagcc 1200
atacgggatg atcggaagga cgcgcagggc aacgtgccgg gtttcggaca gctccacccc 1260
cgctcgcagg gcgtgacgac tctgggcacc atctacagct cctccctgtt cccgggtcgg 1320
gctcccccgg ggcacatgct gctgctcaac tacatcggag gggccacgaa ccgagggata 1380
gtcaaccaga cgcaagagca gcttgtggcg caggtggaca aggacttgcg gttgatggtg 1440
ctcaagcccg acgcgccggc tccacgtgtg gtgggcgtgc gggtttggcc ccgtgccatt 1500
ccccagttca acatcgggca cttggagctg ctggataagg cccggggggc gctggaggcc 1560
aagggctgga atggcgtctt ccttggcggg aactacgtct cgggggttgc cttgggcaag 1620
gtggtggagt acgggtacga gagcgcggcc aaacttgcaa agcacctcac agccgctcag 1680
aagcaggtgg cggtgtaa 1698
<210> 136
<211> 1482
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 57
<400> 136
atggccgtcg cggagggcgc cacacccgcg ccggccgcgg cgtccgccac cccgtcggaa 60
gtcgacgcgc tagtaatcgg ctcgggcatc tccggctcgt cgctggcctt tacgctgtcg 120
caggcctcgc ccgccacgtc gctgctgctc acggaggcgc gccccgtcgt gggcggcaac 180
gtcatctcgc gcaacgagcg cggctacacg tgggaggagg gccccaacac cttccagccg 240
gccccgcaca tcatccgcat ggccgtcgac ctcggcctca gggacgacat cgtcctggct 300
gaccacaccc tgccccgctt cgtgtactgg gatcagcgcc tgttcgccct gccgctctcg 360
ccgaacgaca tccccacctt ccgcctgctc tcgctgcccg gcgccatccg cgccggcctg 420
ggcgccgcgg gctttgtcat gcccccgccc aaggggaggg aggagagcat caaggacttt 480
atcacccgcc acttgggcgc cgaggtcttc cagaagatga tcgacccgtt cgtgtccggc 540
gtgtacgcgg gcgacccgag caagctcagc atgtcggccg ccttcaagaa gatctacgcc 600
ctgcaggagc taggcatgac gcagggcatt gtggagggcg ccatcattcg catccagcag 660
aagaagaagg aggccccgcc gccggacccc gagctgccca cgtggaaggg cggcgcgctg 720
ggctcgttcc gcaaggggct gggcatgctg ccgcaggccg tggcggagcg gctgggcgac 780
cgcgtcaagt tgtcgtggaa gctggtgtcg ctgggcaagg agagcgacgg gcggtatcgg 840
gcgacgtacg agacgcccga ggggacgaag acggtgattg cgcggagcgt ggcgatcacg 900
gcgccggcga acgcgacggc tgggatcctg ggggaccttg cgccgggcat caaggcgatc 960
gaggagatca actacccgac ggtgtggtcg atcacgctgg cgtacccgaa gaacgagttc 1020
cgcgagccgc tgagcgggtt tggaaacctg atcccgcgca gcatggggat ccggacgctc 1080
gggacgatct ggtcgagctg cctgttcccg gggcgcgcgc cggagggcat ggagctgctg 1140
ctgtcgtaca tcggcggcgc gcaggacgcc gcgattaagg agctgacgga ggacgaggtg 1200
gtggccacgg tggacgcgga tatcaagaag attttgatga aggacgggag cgaggttaag 1260
cccgtggtgg tcggggcgcg gaagtgggat cgcgcgattc cgcagtataa tatcgggtac 1320
tgggatatca tgggcaaggc tgaggaggcg gtcaaggagc accccggcat cttcttgggc 1380
ggcaactata cgagcggcgt cgcgttcggg gattgcgtgc aatggggcat tgatacggca 1440
cccaaggttg cagagtatgt cgccgcgcag gcggcggtct aa 1482
<210> 137
<211> 1713
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 58
<400> 137
atgagcagtg gattcttgtg tttttgtcca aacgcggtta tcaaagcagc tcagtatgtg 60
agacctaaaa acactttttc gtgtaagcac tatacctgca aaaatagaaa tagttggttt 120
tacggcaatg agttgtatat aaagagacgt aaagtggtcg aacagcagag aacaactagc 180
cacgtttcta agttgccttt tcgtcaaaca tggaaagctg tttcagctca gcacacggaa 240
gatggcgaat gtagtgtatt gattattggt tctggcgtca caggaagtac tgctgctttt 300
caattggccg aaagtggcat tgatgtctta gtagctgaga aaaatgaaca agtcggagga 360
aatatcattt ctagaaagga gcaaggtttc atttgggaag aaggtccaaa tacattccaa 420
cctactccag atattcttgt tatgatcgaa aagttaggtt tggtagacaa actggtctta 480
gctgatgcaa agctgccacg atatgtgttc taccaggata aacttcataa gattccttca 540
agtccttttg aagcctgcca attttctctg ttgtcgacaa gaggaaaatt gcgtgccttt 600
ctaggagcta ttggctttgc tccaatgaat ctcaaaaaga aagaggaaac cgtaagagat 660
tttgtaacga gacatttggg ggaagaggta tatgaacggc ttgtggatcc ttttattagt 720
ggagtatatg ctggtgatcc ttccaagctg agtatgaaag cagcattcaa acgagttcaa 780
gccttggaag aaaaaggtgt tacccaaagc cttattgaag gtgctattat tcgaatgttt 840
gaaacaaaga acagtaaagg aaagaaagaa acaaaaggtt ctctcaaggt tccaagaggc 900
tctcttggtt cattcaaaga tggcttacaa atgtaccccc aatcagtaca gtcgaagctt 960
tcgaacaaag tgaagactgg atggaagttg ataagactgg agaagagcgg aaatggaagc 1020
agtcatcagt catgtgagga ttactttgca gtgtttcaaa tacccaatgg aatcaccaaa 1080
gtaatacgta cgaaggcatt gatatttact agtcctgctt atatcactgc atctcttttg 1140
agacctttca ttcccaatgc atcagatctg ttggagcaaa tctattatcc ttgtgtagta 1200
tcagtatcct tagcgtatcc ctcttcatct tttcgatttc ctttatctgg ttttggtcat 1260
ttgattccaa gaagtacaaa aattcgtact ttgggtacca tttggtcgag tagtctattt 1320
ccatatcgag tacctgaagg ttatcatctt ttatcctctt acattggagg tgctcaggat 1380
acggatattg cttcgctatc tgaagaccaa gttgtgaaac aagttgattc tgatataagg 1440
aaaatacttt taaggcaaga tgcagctctt ccaaaggttt tgggagtacg tcattggaac 1500
aaggccattc cgcaatatga actaggtcat ctctctcgaa tggaaactat tcaaagtcaa 1560
gtcagtgaaa ctcttccagg tgtgtttttg ggtggtaatt atgtcagcgg aatttctttt 1620
ggagattgtg tgtcatttgg tatgcaactt gctgatcaag catcttacta tatcaaagaa 1680
aatacagaat tgctttcttc gacagtcgtt tga 1713
<210> 138
<211> 1449
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 59
<400> 138
atgacttctg ctcaacccac cgaagttaat caacccttag atgttcttgt cgttggtgca 60
ggaatttctg gcttagcgat cgcccatgaa ctagcgatcg ccaaaaatta ccgtgtttta 120
gtagcagaag ctcaggatcg cgtgggtggt gcaattacca gtaatcgcaa tgatgaaggc 180
tatctctggg aagagggccc taatagcttt caaccagcac cagagttatt acgtctcgcc 240
gtacaggtcg gtctcaagga tgaattggta cttgccgatg ggaaattacc gcgatttgta 300
tttctcaatg gcaagttaaa tgctttgccg atgagtccac ctacagcgat cgcctccaaa 360
atcttgactt ggggtggcaa aattcgtcta gcgctaggtg cattaggttt tgcacgtcct 420
gcgatgagtg gtgaagaatc cgttgatcaa ttcttttcgc gcctactagg taagcaagcc 480
gtggagcgct tagttgcgcc ttttatctct ggagtctatg caggcgatcc taaacgcctc 540
agtgctagag ccgcattttc taaaattttc cgtctagaaa atggctatgg tggattgctt 600
gcaggggcgc tcctgacagc caaagaacgc aaagcccaaa agctgaatga tcccaatatt 660
cccaaggtga agtctggcga actgggatct ttccgccaag gtattaaaat gctgcccgaa 720
gcgatcgcta ccaaactcag agatcaaggt acggctgtca aacaacaatg gactttgcga 780
tcgctagaaa agcaaggcga catctatatt tctcagtttg atacgcctac gggtgcagaa 840
actattacat cgcgatcggt agttctgacc acacctgcct atgtgagcgc taagttattg 900
caagactatt tacccgccgc tagccaagcc ctgagcgaga ttttctatcc gacagttgcc 960
tgtgtcgtac ttgcctatcc caaaagcgaa tttgcctatg acatgaaggg attcggcaat 1020
ctcattcctc gcactcaagg cgtaagaacc ctcggtacaa tttggtcatc gagtttgttt 1080
gcaggacgcg cccctgatgg ttggcaacta ttacttaact ttatcggtgg cacactcgat 1140
cctgctttgg caaaactttc agaagccgag attgttcaag cggtacacca agatctcaag 1200
aaaacaattt tgcgccctga tacgaaagcc gaacctaaag cgatcgcagt ccatgtgtgg 1260
gacaaggcaa ttcctcagta cgagatcgga catttagagc gtctggcgac gatagaagcc 1320
gaattacaaa aatcacaggg tctgtatgtc agcgcgaatt ttattggtgg tgtttcgctt 1380
ggtgattgca ttaagcgggg tctacaagaa gctagtaaaa ttgatgtctt tttaagtagc 1440
caaggataa 1449
<210> 139
<211> 1452
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 60
<400> 139
atgacaaacc tagttgatag ccttatagtt ggtgctggta ttagcgggct aagtttagcc 60
catagcctga accgtgaaaa aaacccccgc tcacccctaa aagttttggt cacagaaagc 120
cagaaccgag tcgggggaaa tattacgacc ggacgcgcat ctgatttttt atgggaagaa 180
ggccctaaca gttttgcccc cacccctgaa ttactcggac tagccgtaga tttggggctt 240
aaacaagaat tgatttttgc cgatcgcaaa ctaccccgtt atgtctactg gaaccataag 300
ttacatccag tacccatgac cccccccgcc ctactctcct cccaactaat cagccccaga 360
ggtaaactcc gcgccgcctt gggagccata ggatttgtgc cacccccagt aggcgctcac 420
ctctctcaac agcgaggaga agaaactatt acccagtttt tccatcgaca tctcggttca 480
gaagtcctag aacgtctcgt ccagcccttc gtttctgggg tttatgctgg cgaccctcaa 540
caattagcag tccgttccgc ttttagtcga ttagtagccg ccgaagacgc agggggtgcg 600
ctactacctg gatttgtgcg atcgcgcctt aataaaaaaa ccaccaaaga caccaccccc 660
gaccccaata ttcccaaaac tcgccccggt gaattagggt cctttcgtta tggcctggaa 720
actctgcccg aaaccttggc cagtaaatta ggcgatcgag ttaagttaaa ttggaccctc 780
gaccgttttt atcccacccc tcatcaaacc tatattgctg aattttccac cccagacggc 840
ccccagcaag tagaaacccg aaccctcgct ttaatgaccc ccgcccatgt tagcgctcgc 900
ctcttgcaac ccctacactc tcaaatcgct tccgcattaa gccaaattcc ttatcccccc 960
gtcgcttgtg tcgtactcgc ctatcctaaa tcagccttaa aacaacaact caaaggcttt 1020
ggtaatttaa ttccccgcca tcaaggaatc cgtaccctcg gcactatttg gacctccagt 1080
ttattccccg gtcgcgcccc agaatcttgg caagtcctca gcaattatat tgggggtgct 1140
acagaccctg aaattggcga aatggatgat gatcaaattg tcgccgccgt tcatcaagac 1200
ctacgccaaa ttctgctggc tgaagatgtc ccccccaaag tcttggctgt ccatctctgg 1260
cggcgcgcta tcccacaata tactctcggt catcaagatc gcctaaattc catcaatgct 1320
ggattgcgat cgcttcccgg actttatctg tgtagcaact atattgatgg ggtttccgtc 1380
ggtgactgtg tgaggcgcgg ccagcaatgg gcatcgcaaa ttcagtccca ccttcatcca 1440
acagccaact aa 1452
<210> 140
<211> 1410
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 61
<400> 140
atgggcagga tgagcgtagc cgcagagccg ctggatgtac tggtagtcgg tgccggcctg 60
agtggtctgg ccctggcctg gcacctccag cgctctggcc ggagggtcct ggtgtgcgag 120
gcgactgagc gggtgggcgg tgcgatcagc tccgaactca tcgatggctt cacctgcgag 180
ggcggtccga acagttttca atcgacccgg cccctgcagg agttgctggt cgaactgcag 240
ctggaggagc ggctggtctt tgccgaggag cggctcgccc gctttgtctg gtgggaaaac 300
cgcctgcggc ccgtgccgat gagccctgcg cagttcgtgc gcgcagatct gctcagctgg 360
ccgggcaaac tgcgctgtct atcggaattt tttgtgccgc ctctttcgga gccgcgcgag 420
gagacggtag ccgaattcgt gctgcgccgc ttcggcgacg aagcgctcaa tcgcctcatc 480
gaaccgttca tcgccggggt gttcgcaggc gacgccgggc agttgagcgc cgatgcggcc 540
ctcgccccgc tcgtcgaact ggaacgccag gcgggcagcg tgctcagggg gctgtggcag 600
cgccgcaatc ggggggtgct cacaccccag cggctgtgta cgctgcgcgg cggcatcgag 660
cagttgccac gggcaattgc ccggcggttg caatcgcagt tgcgcttcca atcgcgctta 720
gaggcgctgg aaccgctggc agggggctgg caggcgattg tcctcgatgg gcggggtgag 780
gcccaggcga tcagtgcccg ctcggtggcg cttgccgccc ccgcttgggc gatcgctccg 840
gtgctggcgc ggctcgatcc gagcctgggc cgcgccctgg agagcatcta ctatccaccg 900
gtggcggcgg tcagcctcgg ctactcaaaa agccagcttc ccaacctgag cgaaggcttc 960
ggccatctca tcccgcgcgg acaatcgctg cgcagcctcg gggtgatcta caacagttgc 1020
ctcttccggc acgccgcccc gacgagctgg cggcttttta cctgtttttt agggggtacg 1080
accgacccgc tcatcgccga tctgagcgac ggtgaactgg ccaatctggc gcaccgcgaa 1140
ctgcaggcgg tcctcgactt tcaatcgagc tatcagctgt tgcgggtggc gcgctggccc 1200
agagcgattc cccagtatgc cctgggccat atcaccaaac aggagcgcat cgagcgctac 1260
ttggcggagc tgccgggatt gtttctggtc ggcaattatt ttggcggcat ctctgtgggc 1320
gactgcgtgc gccaggcccg tctcaaggca gatagcgtgc tgcagtttct cacaagaacc 1380
gctgccaatg gccgcctcaa cctggcctga 1410
<210> 141
<211> 1401
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 62
<400> 141
atgaacacgg ccgaggaatt gctggatgta ctggtggtgg gcgctggcct gagcgggctg 60
gcgctcgcct ggaaactcaa gcgggccggc tgcacgttct tggtctgtca ggcgggcgag 120
cgggtgggcg gagcgatcac caccgcgatc gccgacggtt ttgtatgcga ggggggtccg 180
aacagttttc aagaatcccc acccctcatc gagctgttga cccagttgca actcgaagat 240
caaatcgtca ccgccgatcc gcgcctggcg cgctttgttt ggtgggagaa ccgcctgcgc 300
tcggtgccgc tcaccccggc gcagctggtg cgctccgact tactcagttg gtccggtaaa 360
gcccggctgc tgtgggagtt gttcgtcccg gccctaggcg agccccgcga ggagacggtg 420
gccgaatttg tcctgcgccg cttcggcgaa gaagtcctct cgcggctggt cgatccgctg 480
gtttcgggga tgtgcgcggg cgatgtcggc cagttgagca tcgaagccac cttcgagcgg 540
ctggtcgatc tggagcgccg ccacggcggg gtgctgcggg gtttgtggcg gactgcccgc 600
acccgcccac ccttcaagcg cctctgcacg ttgcgcggcg gcctggagca gttgccccag 660
gcactcgccc agcgcctgca gccgcaaatc ctgctttccc accgcctcga ggcgcttgaa 720
cacctggccg gggatcgctg gcgggcggtg gtggccggtc cgcagggcga gccggtggca 780
attgcggcgc gcacggtgat tctgtcgggt tccgcccacg ccatggcttc ggtcttgcgt 840
cccctggacg ccggtctcgg acgcgccctt gaaagcattt actacccacc ggtcgtgagc 900
atcagtctcg cctactccaa aagccaggtg cccaacgcgc cggagggctt cgggcatttg 960
attccccgca accagaccct gcgcagtctc ggggtgatct ggaacagcag cctctttccc 1020
cataccgccc cacccaactg gcggctctat acctgttttg tgggcggcac caccgatccg 1080
gccaccccca acctcagcga caccgaactg gccagcctcg cccaccgcga actgcagacg 1140
gtcctcggtt tccaggcggg ctatcaactg ctgcgcgtga cgcgctggcc ccaggccatc 1200
ccccagtacg ccctcggcca tcccagcaag caggagcggg tcgaacgggc gctgttggga 1260
ctgccggggt tgttcctggt cggcaattac ttcggcggca tctccctggg agattgcgtg 1320
cgccattccg gtgcggtcgc ctcccgcgta ctgcagtttt taaccacggt ggccagcaac 1380
ggcagcctgc gcccggcctg a 1401
<210> 142
<211> 1617
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 63
<400> 142
atggtcgccg ccgccgccgc cgccatggcc accgccgcgt ccgctggcgt gcctccactc 60
agcgggcccc gcggccccgc gcggctccgc agccgcggcg tcatcgtacg ctgcgccgcg 120
gtggcgggcg gcgccgccga ggcgccgacc tccacgggcg cgcgggtgtc cgcggactgc 180
gtcgtggtgg gcggcggcat cagcggcctc tgcaccgcgc aggcgctggc cacgaagcac 240
ggcgtcggcg acgtgctcgt cacggaggcc cgcgcccgcc ccggcggcaa catcaccacc 300
gtcgagcgcc ccgaggaagg gtacctctgg gaggaggggc ccaacagctt ccagccctcc 360
gaccccgtcc tcaccatggc cgtggacagc gggctgaagg acgacttggt gtttggggac 420
ccgaacgcgc cgcggttcgt gctgtgggag gggaagctga ggcccgtgcc atccaagccc 480
gccgacctcc cgttcttcga tctcatgagc atccctggca agctcagggc tggctttggg 540
gccctcggta tcagaccgcc gcctccaggc cgtgaggagt ccgtggaaga gttcgtgcgc 600
cgcaacctcg gggctgaagt ctttgagcgc ctcattgagc ctttctgctc aggtgtctat 660
gctggtgatc cttcgaagct cagtatgaaa gctgcatttg ggaaggtgtg gcgcctggaa 720
gaggctggag gtagtatcat tggtggaacc atcaagacga ttcaggagag gggcaagaat 780
cctaaaccac ctagggatcc ccgccttccg aagccaaagg ggcagacagt tgcatctttc 840
aggaagggtc tcgccatgct tccaaatgcc attacatcca gcttgggtag taaagtcaag 900
ctttcctgga aactcacgag cattacaaag tcagacggca agggatatgt attggtgtat 960
gaaacacctg aaggggttgt ttcagtgcag gctaaaagtg ttatcatgac gattccatca 1020
tatgttgcta gtgacatctt gcgtccactt tcaagtgatg ctgcagatgc cctttcaaga 1080
ttctattatc caccagttgc tgctgtaact atttcatatc caaaggaagc tattaggaaa 1140
gaatgcttaa ttgatgggga gcttcaagga ttcgggcagt tgcatccacg tagtcaagga 1200
gttgagacat taggaacaat atacagctca tcactcttcc caaatcgtgc tcctgctgga 1260
agggtgttac tcctaaacta cataggaggc gctacaaaca caggaattgt ttacaagagt 1320
gaaagtgagc tggtagaagc agttgatcgt gacctcagga aaatgctaat taaccctaga 1380
gcagtggatc ctttagtcct cggtgtccga gtgtggccac aagccatacc tcagttcctg 1440
gtaggacatc ttgatcttct ggaggctgca aaatctgccc tgggccgagg tggctacgat 1500
gggctgttcc ttggagggaa ctatgtagca ggagttgccc tgggccgatg cgtggagggt 1560
gcctatgaga gcgcctcgca aatatctgac ttcctgacca agtatgccta caagtga 1617
<210> 143
<211> 1692
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 64
<400> 143
atggcagcct ttgtcccggc cgccgcggcc gccccctgga ccgcccgccg cagcgccttt 60
gccggcgccc ccgcggccgc cggcagccgc cgccccgcca tggccggcgc ggcggcgccg 120
ctgcggtcgc tcgtccctcg ggccatcccc ccgccggggg cggcccggcg cgcggcgccg 180
tcgcctcgca tggccgccgg cggcgcgccc gacgaggtcg tcgacgcgct tgtcgtcggc 240
tcgggcgtgt ctggctccac cctcgcctac cgcctgcact ctcgcggcgt ttcaatgctc 300
ctcaccgaag cgcgcgacgt ggtcggcggc aacgtcatct cccgctcggc caatgggtat 360
acgtgggagg agggccccaa caccttccag cccgcgcccc acatcctgcg cctggccgtc 420
gacgtgggcc tcaaggacga cctggttttt gccgaccaca cgctgccccg ctttgtctac 480
tggaatggca acctgtttgc gctgccgatg gggccggcgg acatccccaa gttccgcctc 540
ctctccccgc tcggcgccgt gcgcgcgggc ctcggcgcgg cgggctttgt gtggcccaac 600
tggtccggca aggaggagtc ggtcaaggaa ttcattacgc ggcacctcgg cgcggaagtg 660
tttgccaaga tgatcgaccc gtttgtgtcg ggcgtctacg cgggggaccc gtcgtcgctg 720
gccatccgcg gcgcgtttgg caagatctac gccctccaaa acttgggcat cacgcagggc 780
attgtggagg gcgccatcat ccgcttgcgg cagcgcaagg cagaggcggg ggagccagac 840
ccagagctgc ccgtcgtcaa gggcggcgcg ctcggctcct ttcgagaggg cctcgggatg 900
ctgcccaagg cggtggccgc caagctcggt gacgcggtgc ggctcggctg gacgctcacg 960
gagctgcgca agctcccggg ctcggcgccg ggctatgagg ccgtctactc aacgcccgac 1020
ggcccacgga cggtgcacgc caagaccgtg tcgctgacgg cgcccgccgg cgcggcgtcg 1080
tccatcctcg gcgggatgct ccccgctgcc aaggccctcg acgacgtcta ctacccgtgc 1140
gtttggtcag tcacgctctc ctacccgacg gccgcgttta agcggccgct caagggcttt 1200
ggcaacctca tcccgcgctc catgggcgtc cgcacgctcg gcaccatctg gtcctcgtcg 1260
ctcttccccg gccgcgcgcc gcccgggcgg gagctcctcc tctcctacat tgggggcgcg 1320
caggacaagg ggattgccga cttgtcagag gaggaggtgg tggctgccgt ggacggggac 1380
atcaaggcgc tgctgctgtc ggagggggcg gccgacgaag tgccggtcgt cgtgggcgtc 1440
cgcaagtggc cccgcgccat cccccagtac gtgcgggggc acctcgagtt ggtggcgggc 1500
gtgcgggagg cggccgccgc cgagtgcccg gggctgtttt tgggcggcaa ctacgcgtcg 1560
ggggtggcgt ttggggattg cgtcgcgtgg ggcgacaagg ccgcggcaga ggtggttgcg 1620
acgctcgggg acatgcccga gacgggggcg cccatggcca tgccagagac ggcgacgccg 1680
gcgagcgtgt ga 1692
<210> 144
<211> 1602
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 65
<400> 144
atgtcgcgcg cgcaggtctc gctcgcgccc gcgcgcgtcg ccgatcggcg ccgcgtcggg 60
cgcactcgcg ccgcccgacg cgccgtcgcc gtccgcgcgt cgacggtgga acgcgacgtc 120
gtcatcgtcg gcgcgggcgt cagtggtttg tccaccgcgt tcacgctggc gaagaagacg 180
atgccgaacg cgagcgtgat ggtgaccgaa gccagagatc gcgtcggtgg gaacattacg 240
agtaagagcg atggcacgta cacgtgggag gaggggccga actcgtacca acctggggat 300
gcgatcctca cgctggcgtg cgacgccggc atgcgggacg acatcttatt ggccgatccc 360
gcgagcaatc gatacgtgct gtgggatggg aaattgagaa ttttgccgca cagcatcgag 420
agtgcggttt tgggggattt cttgacgtgg ccggggaaga ttcgagccgg tttgggggcg 480
atcggaatca ggccgccggc gccgggtaag gaggagagtg tgaaggaatt cgtgagcaga 540
aatctcggca cggaggcgtt cgagcgattg atcgaaccat tttgctcggg ggtgtacgct 600
ggggatcccg cgagcttgag ctcggtcgcg gcgacggggc gagtgcagcg acttgaaccg 660
ctgggggggt cgctcgtggt gggcgcgctc aaggcgcagg cggaggcggc gaaggcgaag 720
aaggagagcg ggttcaagcg cgatcctcga cttccggaag tgaagggtca aaccgtaggt 780
tcgttccgag gtggtttgaa gacatttcca gagggattag cgaaacaact cggcgacgac 840
gtcgtcaagt gcaactggaa gctcgtcaat gtcaacaagg ctgccgaggg cgggtacacg 900
tgcgagtacg ataccccgga ggggcgacga acggttatcg ccaagtgctt gttgctcacc 960
gctccggcgt acgtcaccgc tgagatcgtc aaggacatgg ctccggcggc ttcgacggcg 1020
ctcaacaagt tttactaccc tccggtggcg tccgtgacgg tttcgtacaa gaaggactcg 1080
ttccgtcttg acggcacttc agcgcttcct gagggtggtc tcactggttt cggtcagctc 1140
cacccgcgct cgcagggcat ccgcacgctc ggcacgattt actcgtcctc gcttttcaag 1200
gacgacaagc gccagccgga tgacgagttc atgatcttga actacatcgg tggcgcccga 1260
gacgtcgcga tcaaggactt gagcgaagat gagctcgttc agcaagtgca cgaggacgcg 1320
ctcaagacga tcctcaagcc cggaacgccc cttcccaagg ttgtcggcgt caagctgtgg 1380
gagaaggcca tcccgcagtt caacctcggc catctcgacg tcttggccga ggccgagaac 1440
gcgctcgagg cggcggcgtg cggcgaaaag gatggtctct ttctgggcgg taactacact 1500
gccggcgtcg ctctcggtag atgcgtcgaa tttggcgttg agcaagccga cgaggtcgcc 1560
acgttcctca agtcggcgaa gaagctaact gccgcggttt aa 1602
<210> 145
<211> 1656
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 66
<400> 145
atgctcgccg ccgttggcac cgccgctggc ctcctagccg tagcggggat cacccccgct 60
tatggtttcg taacgccctc caccgcttgg aacaccgctc gcgggagcgg ccatggaacg 120
gcgtcgagag cagccactac catggcagct gatattttca aggccggccc tgtcgaaaag 180
gtggactgtg cggtggtcgg ctctggcatc agcgggagca cgctggggtt ctacctggac 240
aagaagggcg tggactgcgt ggtgctagag gcccgggatc aagtgggcgg caacgttata 300
tccaagaaag aagacggatt cctgtgggaa gagggcccca actcgttcca gccgacaccc 360
tacatcatgc ggaccacggt cgacctgggc ctcaaggagg acctcgttct ggcggacccc 420
accctgcccc gcttcgtgtt ctgggaggga ggcctgttcc cccttccctc ttccctgcag 480
tcgatcatta cggacttttg gttgctttca tggcccggaa agatcagggc cgggctgggt 540
gccatcgggc tggtgctgcc ccccccttcg gactacgagg agagcgtaaa agagttcgtt 600
actaggcacc tggggccaga ggcgttcgaa aggctcatcg accccttcgt gagcggggtt 660
tacgcgggtg acccgtccaa gctggccatc aaggccgccc ttaagaaggt cgctcggttg 720
gaagtgttgg gcggtccggg cttgatcgac ggtgctattc tcaggcttaa ggagcgtgca 780
cggcaggaga aggagctccc ggagccgctg caatcggagg accttcccac gtaccaggga 840
ggcagcctgg gctctttccg cgaggggctg cagatgctcc ccaacgctgc tcttaaggcc 900
atggggaaag acaagatgcg gacgtcgtgg gtgatgaagg gcatcaagcg gtcagaggac 960
ggcggctacc tcctcgcctt cgacaccccc aaggggccca aacgcctcca ggctaaggtt 1020
gccgtttgca ccgcgccggc ccacaggctg gcctccgtcg agggactgcg ggatattgtg 1080
ccggaagcgg cgcgattgga cgaggtgtac tacccccccg tggcgagcgt aaccctggcg 1140
taccccaagt cgtccttcaa ggtcgacctc accgggttcg gcaacctcat cccgaggaag 1200
atgaagatca ggaccttggg cacgatctgg tcatcgtcgc tattcccagg gagagcccca 1260
gagggctacg ccatgctgct caactacatc gggggtgccc aagaccccgc gatcaaggac 1320
ctttcggacg atgagatcgt ggcggagtgc gacagggaca tccgcacaat ccttctcaag 1380
gacgatgcgc ctcccccgaa ggttctcggc tgccgcttgt ggaaaaccgc catcccgcag 1440
taccagaggg gccacctggc catcctggag gagctgcaag agggactcaa ggccgccccg 1500
gggcttcgta tggggggaaa ctacatcacc ggagtggcct ttggagactg cgtgcagtat 1560
gggtacgaag aggcggagcg gatcgaggag atgctcaaga gcggtgcctt ggacgggcag 1620
cagagcgtgt ccgacaaaga agcagttgca gcgtag 1656
<210> 146
<211> 1422
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 67
<400> 146
atggaggaaa ccgacgttct ggtggtgggc tctgggatca gtggctcgac caccgcgttt 60
tatctgaaca aagctggagt caagtgtctg ctgacagagg ccaaacccgt ggtgggaggg 120
aacgtgatca gtaaatcgga gtccggcttc ttgtgggagg agggcccgaa ttccttccaa 180
cccacttatc cgctcatgca agcgactgta gacatgggcc tggccgatga actggtcctg 240
gcagacgcga gcttgccccg cttcgtgtat tggaaggaga aactctacgc cctgcctggg 300
ggcttgggtg acatcccctt cttcaatctc ctatccatac ccggacgtat cagggcgggt 360
ctgggggctc tgggcttcat tcgaggcccg ccgaaggata aggaggagag cgtcaaggaa 420
ttcgtgaccc ggcatttggg ggcagagacc ttcgagcgga tcatcgaccc cttcgtctcg 480
ggcgtctacg ccggggaccc gagcaagctc tccatgaagg cggccctgaa gaaggtgaaa 540
cgcttggagg agctgggagg gaggggcatt ctggacgggg ctttgcttcg catccaggag 600
atccaacgga ccaagccgcc ggtggtgccg gagcaccctg tgtacaaggg cgggcagctg 660
ggttcattca agcgaggctt gcagtcgatg cccctggcgg cggccaaggc gctggggaag 720
gagaaggtcc ggctctccca caagctcttg agcgtggtgg aagggaaagg gccgaagggt 780
ggctacgagg cggtttttca gaccccccag ggacggaaac ggatcaaatg ccaggcccta 840
gccatcaccg cccccgccca cgttgtgcac aagctcctgc gtcctttggt cccggaggcg 900
gcccgtcttg ccgacgtgta ctatcccccg gtcgcctccg tcaccttggc ctacccgaaa 960
accgcctttc gggagccgtt gcggggtttc ggaaatttga ttccccggag catgaagatc 1020
cgaacgctgg ggaccatctg gtcgtcctcc ctcttcccgg gcggcccccc cgactacaac 1080
atgctgcttt cctacatcgg aggggcccaa gatccgggta tcgcggagct gagcagccag 1140
cagatcgtca aggaggtaga cagggatatc aagaaggtct tgctcaagcc cgacgccccg 1200
gcgcccaaga tcctgggcgt gcggctgtgg cccaccgcca tcccgcagta caacaagggc 1260
cacctggaca tcctcgcgag cgtggaggca ggggtcaaga aacatccggg tctctttttg 1320
ggggggaatt accgaacagg cgtggcgttt ggggactgcg tgacctacgg gatggaggaa 1380
gcgggcagga ttcagagcta cttggctgct aaggctccgt aa 1422
<210> 147
<211> 1419
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 68
<400> 147
atgaaaaaga tgcgcgaggc gacggtgctc gtcacggagg cgcgcgaacg cgtcggaggg 60
aacgtcacga gtcgatcgga tgggacgtac acgtgggagg agggaccgaa ctcgtatcag 120
ccgggggatg cgatcttgac gctggcgtgc gacgcgggga tgcgcgacga catcttgctg 180
gcggatccgg cgagcaatcg gtacgtgctg tgggacggga agctgcggat tttaccgcac 240
agcattgaga gcgcggtgtt gggggatttt ttgacgtggc cggggaagat tcgggcggga 300
ttgggggcga tcggaatcag gccgccggcg ccgggcaagg aggagagcgt gaaggagttc 360
gtgagcagaa acttggggac ggaggcgttc gaacgtctga tcgaaccgtt ttgctcgggc 420
gtgtacgcgg gtgatcccgc ggcgctgtcg tccgtggcgg cgacggggcg cgtgcaacga 480
ctcgaaccgc tcggcgggtc gctcgtcgcc ggcgccatca tggcgcaaaa ggaggcggcg 540
cagaacaaga aaccgcgcga tccgcgcctt cccgaggtca agggccaaac cgtcggctcg 600
ttccgcggtg gtttgaagac gttcccggag ggtttggcca agcagctcgg cgacgacgtc 660
gtcaagtgca actggaagct cgtcggcgtc tcgaaatccg ccgagggcgg gtacgagtgc 720
gcgtacgaca ccccagaagg gccgcaaacg gtgagaacga agtgtttgtt gctcaccgcg 780
ccggcttacg tcgcggccga gatggttaag gacatggctc ccgcggcggc gacggcgctg 840
aacaagtttt actacccacc cgtcgcgtcg gtgacgattt cctacaagaa ggattcgttc 900
cgcctcgacg gcaccagcgc gttacccgaa ggcggcctca ctggcttcgg tcagctccac 960
ccgcgctcgc aaggcattcg cacgctcggc acgatttata gctcgagcct gttcaaggac 1020
gacaagcgtc aaccggacga cgagttcatg attttgaact acatcggcgg cgcgcgcgac 1080
gtcgcgatta aagacttgag cgaggaggag ctcgtgaagc aagtccacga ggacgccttg 1140
aagacaatct tgaagcccgg tacgccgctt cccaaggttg tcggcgtcaa ggtttgggaa 1200
aaggccatcc cgcaattcaa cctcggccac ttggacgtcc tcgccgaagc cgagaacgcg 1260
ttaaccgccg ccgactgcgg cgaaaaagac ggcttgttcc tcggcggcaa ctacaccgcg 1320
ggcgtcgcgt tgggtcgatg cgtcgagttc ggcatcgagc aagcggacga agtcgtggcg 1380
tacttaaacg ccgcatccaa gaaggccgtc gcggtgtga 1419
<210> 148
<211> 1713
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 69
<400> 148
atggtgacga tggcggcggt gaggacgaag gcttgggtga ccagcccgtc catgctgagc 60
tctcctgctc tcctcaggat gccttcctgc tcatggaccg gcaagtcctc tgtaagaggc 120
gctagagaca gctcggtcca gttccgggga aggtcggctc ggttcggagc tggggttcga 180
gggatgcaat ctgcgggagg atggggagcg gggagcatgg agaagtacga cacacttgtt 240
attgggagcg gcgtgagcgg ctcttctctt gggttctccc tgttccagaa gggcgtcaac 300
gtccttgtta ctgaagcaag ggacgtggtg ggaggaaacg tgatcaccag agagcaggac 360
gggtttcttt gggaggaagg acccaacacc ttccagccca ctcgccagat catgcgcctt 420
gctgtcgatc ttggcttgaa ggacgagctt gtgtttgctg accacaccct tcctagatgt 480
gtgtactggg agaaagagct cttccccctc ccctccaagc ccgaggatgc gccgttcttc 540
cgcctgctct ccatccccga gaagatccga gctggaatcg gagcgatcgg cctgcatgcg 600
ccgaagccag actacgagga gtcagtgaag gacttcatcg agcgacacct tggcgaggca 660
gtgttcaaga agatgatcga ccccttcgtg tcgggagtgt acgcgggtga ccccaccaag 720
ctctccatgg cctcggcctt caagaagatc tacgcgctcg aggacctcgg catgacccca 780
agcttgatcg agggaggcat catccgccag gcggagaggg ccaaggaggc cagggaaaac 840
tatgaccctg agctgccaac ctacaaggga ggggctctcg gctccttcag gaagggtctt 900
gtcagtctcc ccaaggctgc ccaggagaag ctcggagatc gcctgaggac cagctggaag 960
gttgagtcca tctccaaggg agaggatggg ggatacgtca ccaagttttc gaccccccag 1020
ggttccaagg aggtttggtc caagactgtg gccgtgactg ctcctgctca tgctaccgtg 1080
ggcatgctct ccgagcttgt gcctgagtgc aaggcccttg aagagatcca ctacccgtgc 1140
gtctactccg tcactctcgc ctaccccaag gagtgcctca aggacgaggt tcgcaaagag 1200
agaccgggcc tcggcaagag gctcttcggg ttcggaaacc tcatcccccg cagcatgggt 1260
atccgtacct tgggaacgat ctggtcctcc tcgctcttcc cctaccgtgc tcccgagggc 1320
tacgagatga tgttgagcta catcggagga gcccaggacc ctctccgcta caaccctccc 1380
atcgccgagc tctcggagga agaggtggca aagattgtcc atggtgacgt cagcaagatc 1440
ctcctcaagg aaggagctcc tgagccaaag gtcctgggag tgcgcaagtg gcccaaggcc 1500
atcccccagt acaacaaggg atactcggag attatgggca aggtcaacag cggtctcagc 1560
aagtgccctg ggctgtacct tggaggcaac tacgtctctg gcgttgcctt cggcgactgc 1620
gttcagtggg gagtcgacac tgcacccaag gtcaaggagt tcctcgactc cgtcccttcc 1680
tccgagaagg cggccaacgc tgcgctggtg taa 1713
<210> 149
<211> 1728
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 70
<400> 149
atgctcaggt ggtccggatc tcaacaggta accgaccgga accgagccga acagcatggc 60
ctggtcgtgg cgttcgcttt tcctgctcga gcaccgcgcc acagcgaagc aacacacgag 120
cgctggactg ggctggcgca gcgcgaacgc ggcttgtgtg taccgttgaa agcgaccaag 180
tggaaacgag acttgtggca ctcgcgaaag cttcaaatgc aggagcctgc acagcggaac 240
agagagcagg tgcaagcgct agtagtaggc tctggagtga ctggctcttc gttcgcgttc 300
ctgctgcagc acgctggaat aagcgatgtg atttgcaccg aggcacgagg tgaagttggt 360
ggaaacctta tttcgcgttc caaagacggc tacctttggg aagaagggcc caacaccttc 420
caaccaacac cggtgattct gaaactcgcg cgagacgcgg gtctcgagtc cgagcttgtc 480
ttcgccgacg ccaaactccc tcgatacgta tactgggagg gggttttgca cgcgctcccg 540
agttcgcctg cagacctgat caccggccgt ttccgcctgt tatcgaatgc ggggaaggct 600
cgggcagcgc tcggagcgct cgggttcgta ggcgcaccca aacgcgtcgc acggcaagct 660
ggacccgagc ccagcgagga tgctgaccta gcagacgagt ctgtagagga attcgtgact 720
cgccatctag gccgcgaagt tttcctgaag attatagacc ccttcgtgag cggtgtgtat 780
gcaggcgatc ccagcaaact ctccatggct gcagctttca agcgcgtgta cgcactcgag 840
cagcttggcg gaacacgcgg gattctcgaa ggggcgctca tccgtctgca acaacgtcga 900
cgggaacgtc aacgctgggc ggcagagaat ctccccaagg tgaaagcggg tgcactgggc 960
tccttccgcg agggcctgca gcagttgccg ctcacagtgg cgactcggct gggccctgag 1020
cgaatgcgaa ctcggtatgc actgcgccaa attgaataca atggagcaaa acgtggaaaa 1080
cgtcgctata ctgcccgctt cgaaacccca gatggcgaac gagttatcga aacagatgcc 1140
ctcattctta cgatccccgc acatgcggcg tcaccgctct tgcaaggtct gggtgtctct 1200
gggagtgagc tgctccagga aatcgacttt ccgccggtat actcggtaac tttggcatac 1260
ccaaagtttg cagcgcggtt tcccctaaac ggtttcggga acctgatacc gcgctctgcc 1320
ggtattcgca ccctcgggat ggtctggtcg tcgagtttgt ttccggaacg agcgcctccc 1380
gatatgaata tggtgctctc atacatcggc ggtgcgcgtg atcctggtat ccgcgaatgc 1440
accccggatg aagtcgctgc cctcgtagac gcggacatgc gtcgcgtcct cctacgtgcc 1500
gatgcacctt cgccgcaagt tcttggtgtg agactttggc cgcgggcgat tccacaatac 1560
aaccggggtc acttgcggcg cttggaagcc tgcaaccagg gtctacaagg ctttcctggt 1620
ctctttttag gcggaaacta tctgagtggc gtttccttcg gtgactgcgt acagtgggcg 1680
tacgataatg tgcctcgagt gctgacgttt ctgcaggaga cggcttga 1728
<210> 150
<211> 1680
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 71
<400> 150
atgacgatga cgacggggat atcctcgatt ggaaccaaga agaacagcat taaccgcgcg 60
aaggtgtcgt cgacgagaag aacacgaaga gtccgagctg ctctttccag cagcggcagc 120
aaagggagca actttgtggt gcgcgcgtct tcgtcttcgt cttctccttc tgacgacgag 180
aacaactctt tgggtattaa agatacgatc atcgtcggtg gtggtgtctc tgggttgtcc 240
acggcgttta cgatgaaatc gaagaacgca tcgtgcgata tcatggtcac ggaaatccga 300
gaccgcgtcg gagggaacgt gacctcgaag aatgacggtc aatacatatg ggaggaagga 360
ccgaactcgt accaaccggg agacgcggtg ttgaagttag cgtgcgacgc cgggatgaag 420
gaggatatcg ttctcgcgaa cccggattcg gataggtacg tgttgtggga tggggagttg 480
agagcgttgc caaaggatat accgaccgca gtgctcgggg attttttgac ctggcccggg 540
aagattcgag ctggtttagg ggcggtgggt attcgcatgc cgaaagagga aggtaaggag 600
gaaacggtga aggagtttgt atctagaaat ttaggcgaag aggcgtttca gagattaatt 660
gaaccgtttt gctcgggcgt gtacgccggt gatccggcga tgttgagcgc ggaagccgcg 720
acgggtagag tttcggtatt agagaataaa ggtccgtggc cgggtttgtt tccgggcgcg 780
ttgaaagcgc aatacgaagg cgcgaagaag aagaaggaaa atccgagaga tccgagattg 840
ccggtgattg agggtcaaac ggttgggagt tttaaaggcg gtttgcaaac gttaccggaa 900
ggattggcga agcagttagg agagggcatc gtgaagttgc agtggaagct gatcaaaacg 960
gaaaaaaccg aggacggtct gttctctttg acgtacgaaa ctccagaagg ggagaagaaa 1020
gtgaaggcga aatccgtcgt gtttacgcaa ccggcgtacg tggtcgcgga tacggtgcga 1080
agtatcgcgc cagaagctgc gaaatcgttc gagaagttct actatccacc tgttgcgtct 1140
gtcaccgtcg catacaaacg cgacgcgttt aaattagacg gccgttcagc cttgcctgaa 1200
ggcggcttga ccggtttcgg tcaactccat ccgcgaacgc aaaaagtgcg cacgctaggc 1260
accatctact cctcctactt atgggccgac gacggacgat gccctaaaga cgaattcatg 1320
atcctcaact acatcggcgg cgcccgagac gtcgaaattc aaaaactcaa tgaagacgaa 1380
ctcgtccaag ccgtccacgc cgacgcttta aaaaccatct taaaaccaga cacgccgttg 1440
ccgaaaaaag ttggcgttcg catgtggtcc aaagctatcc cgcagtttaa cctcggtcac 1500
tggaaactct tagacgaagc taaagaactt ttgaaaaaag aaaagtgcag cgaagaggac 1560
ggtttatttt taggcggaaa ctacgtcgcc ggcgtcgcgt ttggccggtg cgtcgaatac 1620
ggcgtcgatc aagcctcgga cgtgttgaac tttttagata agcagagacg aaccgtataa 1680
1680
<210> 151
<211> 1416
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 72
<400> 151
atgcatcaca tgccaagaac aactggaatg aatgttgcag tagttggagg tgggatttct 60
ggtttggcag ttgcacatca tttgagatct aggggtactg atgcagtact tctcgagtca 120
tctgctagac ttggaggtgc agttggaact catgcacttg ctggatacct agtagaacaa 180
ggtcctaata gttttcttga tcgtgaacca gcaactcgtg ctctagcagc agctcttaat 240
cttgaaggac gaattcgcgc tgctgatcct gctgcaaagc gtcgatatgt atacactcgt 300
ggtagactcc gatctgtacc agcttcacct ccagcatttc ttgcatcaga tattcttcct 360
ctaggtgctc gattgcgtgt tgctggagag ctcttttcac gtagagcacc tgagggtgtt 420
gatgaatctc tagctgcatt tggccgacgt catctaggac atagggctac gcaggtactt 480
ctcgatgcag ttcagactgg tatctacgct ggagatgtgg aacaattgag tgtcgctgca 540
acttttccta tgctggttaa gatggaacgt gaacatcgaa gtcttattct cggtgcaatc 600
cgcgcacaaa aggctcaacg tcaggcagcg ctcccagctg gaacagcacc gaaattgtcc 660
ggagcactta gcacgtttga tggtggattg caagtgctca tagatgcgct tgcagcttca 720
ttgggtgatg cagctcatgt aggagcacgc gttgaaggac tagcacgaga ggatggcgga 780
tggagactta ttatcgaaga gcatggtcgt cgcgcagagc tctctgttgc acaagtggta 840
ttggcagctc cagcgcatgc tactgctaaa ttgcttcgtc cactcgatga tgcgctagcg 900
gcactcgtag caggtattgc ttatgctcct atcgcagttg ttcatctagg ttttgatgca 960
ggaacacttc cggctcctga tggttttgga ttcctggtgc ctgcagaaga gcaacgtcga 1020
atgcttggtg caattcatgc ttctactact tttccatttc gagctgaagg tggacgtgtt 1080
ctctattctt gtatggtggg aggcgcgcgt caaccaggac tggttgaaca ggatgaggat 1140
gctttggctg cactagcacg agaagaactg aaggcacttg caggcgttac agctcgccct 1200
tcatttactc gagtgtttcg ctggccactt ggtattcctc aatacaatct cggacatctt 1260
gaacgagtgg ctgctattga tgcggcacta caacgtttgc cgggccttca tctaattgga 1320
aatgcataca agggtgttgg actaaatgat tgtattcgca acgcagctca actagcagac 1380
gcgctcgttg ccggtaatac ttctcatgca ccgtag 1416
<210> 152
<211> 1410
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 73
<400> 152
atgcaccaca tgcagaggac acatcggatg aatgtcgccg tcgtgggagg tgggatttcg 60
gggttggcca tcgcgcacgg tttgcggtcg cgcggtacgg ctgccgtgct cctggagaca 120
tccgcacgtc ttggaggcgc ggtgggcacg catgcgcggg ctggctacct ggtggagcag 180
ggccccaaca gcttcctgga ccgcgagccc gcgacgcgag aactcgcggc ggcgctgaac 240
ctggaaggtc gaatccgggt cgccgaccct tcagcgaagc gtcgctatgt ctacacgcga 300
ggccgacttc ggtcggtgcc ggcttcgccg ccggcgtttc tggcttcgga cattcttccg 360
ctgggcgcgc ggctgcgggt cgccggcgag ctgttctctc gccgcgcgcc ggaaggcacg 420
gacgaatcgc tggccgcgtt cggccgccgc cacctgggcc gcgcggcgac gcgggtgctg 480
ctggacgcgg tgcagacggg catctacgcc ggtgacgtgg agcagctcag cgtcgaggcc 540
accttcccga tgctggtgaa gctggagcgc gagcaccgca gcctcatcct gggcgccatc 600
cgcgcgcaga aggcccaacg caaggcgctg cccgcggggg acgcgccgaa gctgtcaggc 660
gcgctgagca cgttcgacgg cggcctccag gtgctcatcg acgcgctggc cgcatcgctg 720
ggtgacacag cgcacgtgag cgcgcgggtg gaaggcctga cgcgcgtgga tggcggatgg 780
aagctcgccg tcgaggaaca tggacgccgc gcggagctga ccgcgaacca cgtggtgctg 840
gcggttcccg cgtttgtcgc cgcgcagctg ctgcgccccc tggatgacgc gctggcggag 900
caggtgtccc gaatcgagta cgcgcccatc gcggtggtgc acctgggctt cgacgcgggc 960
gcgcttccgg caccggacgg cttcggcttc ctggtgccct tcgaggagaa gcggcggctc 1020
ctgggcgcca tccacgcgtc caccaccttc cccttccgtg tcgagggcgg ccgcgtgctc 1080
tacacctgca tggtgggcgg cgcgcgtcag ccggacctgg tgaagcggga cgaagcagcg 1140
ctcgcggcgc tggcgctcga ggagctgcag gccctgacgg gcgtgacggc ccggcccacc 1200
ttcacggagg tcttccgctg gccgcggggc atcccccagt acaacgtggg gcacctagcg 1260
cggatggcgg gcatcgacgc ggcgctccag cgctggcccg ggctgcacct ggcgggcaac 1320
gcctacaaag gcattggcct caacgactgc atccgtaacg cggcgcggct cgcgactgcc 1380
ctcgcggacg aggaaagcgt tcggaaatag 1410
<210> 153
<211> 1392
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 74
<400> 153
atgaatgtcg ccgtcgtggg aggtgggatt tcggggttgg ccgtcgcgca cggtttgcgt 60
tcgcgcggta cggctgccgt gctcctggag acatccgcac gtcttggcgg cgcggtgggc 120
acgcatgcgc gcgctggcta cctggtggag caggggccga acagcttcct ggaccgggag 180
cccgcaacgc gcgccctggc ggcggcgctg aacctggaag gccggattcg cgtcgcggac 240
gcgtcggcga agcgtcgcta tgtctacacg cggggcagac tccggtcggt gcccgcgtca 300
ccgcccgcgt tcctgacttc ggacatcctg ccgctgggcg cgcggctgcg cgtcatgggc 360
gagctgttct ccagccgcgc accggagggc acggacgaat ccctggccgc gttcggccgc 420
cgccacctgg gccccgtggc gacgcgggtg ttgttggacg cggtgcagac gggcatctac 480
gccggggacg tggagcggct cagcgtcgag gccaccttcc ccctgctggt gaagctggag 540
cgcgagcacc gcagcctcat cctgggcgca atccacgcgc agaaggccca ggcgcgggcc 600
aaggccctgc tgccccccgg tgacgcgccg aagctgacgg gcgcgctgag caccttcgac 660
ggcggcctcc aggtgctcat cgacgcgctg gcctcgtccc tgggagacgc ggcgcacgtg 720
ggcgcgcggg tggaggggct gacgcgcgtg gacgggggct ggaggctcgc cgtggaggag 780
cacggacagc gcgcggagct gagcgcgtcc cacgtggtgc tggcggtgcc cgcgcacgtc 840
gccgccgagc tgttgcagcc cctggatgac gcgctggcgg cgcaggtgtc ccgcatcgaa 900
tacgcgccca tcgcggtggt gcacctgggc ttcgacgcgg ggacgctccc ggcgccggac 960
ggcttcggct tcctggtgcc cttcgaggag cagcggcggc tgctgggcgc catccacgcg 1020
tccaccacct tccccttccg cgtcgaaggc ggccgcgtgc tctacacctg catggtgggc 1080
ggcgcgcggc agccggacct ggtgcagcgg gatgaagcgg agctcgcggc gctggcgctg 1140
gaggagctgc gcgccctggc gggggtgacg gcccggccca ccttcaccca ggtgttccgc 1200
tggccgcgcg gcatccccca gtacaacgtg ggccacctgg agcgcatggc cggcatcgac 1260
gcggcgctcc agcgctggcc cggcctgcac ctggcgggga acgcgtacaa gggcattggc 1320
ctcaacgact gcatccgtaa cgcggcgcgg ctcgcggcgg ccctttcgga cgaggaaaac 1380
gttcggaaat ag 1392
<210> 154
<211> 1410
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 75
<400> 154
atgcaccaca tgccgaggac acacggaatg aatgtcgccg tcgtgggagg tgggatttcg 60
gggttggccg tcgcgcatcg tttgcgttcg cgcggtacgg atgccgtgct tctggagacc 120
tccggccgtc ttggaggcgc ggtgggcacg catgcgcgtg acggctacct ggtggagcag 180
ggccccaaca gcttcctgga ccgcgagccg gccacgcggg aactcgcggc ggcattgaac 240
ctggaaggtc gaatccgggt ggcggacccg ttggcgaagc gtcgctatgt ctacacgcgt 300
gggcgactcc ggtcggtgcc gtcctcgccg cccgcgtttc tcgcatcgga cattcttccg 360
ctgggcgcgc ggctgcgtgt cgcgggtgaa ctgttctccg gccgcgcccc cacgggcatc 420
gatgaatcac tggccgagtt cggccgccgt caccttggac gcaccgccac gcaggtgctg 480
ctcgacgcgg tgcagacggg catctacgcg ggcgacgtgg agcagctcag cgtcgccgcc 540
acgttcccca tgctggtgga cctggagcgc aagcaccgca gcctcatcct gggcgccatc 600
cgcgcgcagc aggtccaacg ccgggccctc tccgcgggcg gcacgccgaa gctgagcggc 660
gcgttgagca cgttcgacgg tggactccag gtgctcatcg acgcgctggc cacgtcgctg 720
ggggacgcgg cgcacgtggg cgctcaggtg gaatgcctga cgcgcgtgga cggcgggtgg 780
aagttgatgg tcgaggagcg cggccagcgc gcggagctga gcgcatccca ggtggtgctg 840
gcggtgcccg cgcatgtcgc ggcggagctg ctccggccct tggatgacgc gctcgcggcg 900
caggtggcgc gcatcgacta tgcgcccatc gccgtggtgc acctgggctt cgacgcgggg 960
acgctgcccg cgccagacgg cttcggcttc ctggtgccct cgggcgaaaa gcggcggctg 1020
ctgggcgcca tccatgcgtc caccaccttc cccttccgcg tcgagggcgg gcgcgtgctc 1080
tacacgtgca tggtgggcgg cgcgcggcag ccggagctgg tgcggcagga cgaagcggcg 1140
ctcgcggccc tggcgctcga ggagctgcgc gccctggcgg gcgtgacggc gcagcccacc 1200
ttcacgcagg tctaccgctg gcagcgcggc atcccccagt acaacgtggg gcacctggaa 1260
cggatggccg gcatcgacgc ggcgcttcaa cgctggcccg ggctgcacct cgcgggaaac 1320
gcgtacaagg gcattggcct caacgactgc attcgcaacg cagcgcggct cgcggatgcc 1380
ctcacggacg aggaaagcgt tcggaaatag 1410
<210> 155
<211> 1386
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 76
<400> 155
atgactgtcg ccgtcgtagg aggtgggatt tcaggtctgg tcgtcgcctg gcgattgcgc 60
tcccgcggta gggatgccgt tctcctggag acaacctccc ggctcggcgg cgcggtgggc 120
acgcgcgcgc agcggggctt cctcctggag acgggcccca acagcttcct ggaccgcgag 180
cccgccatgc gagagctggc atccgcgctc aatctggaac acaggattcg cgcggcggat 240
ggggcggcga agcggcggta tgtctacacg cgggggaagc tgcgctcggt gcccgcatcg 300
ccgccggcgt tcctgaagtc ggacatcctc cccttcggcg cgaagctgcg ggtgatgggg 360
gagctgttca gcggccgcgc ggcgcccggc gtggacgaat ccctggcgga cttcgggcgt 420
cggcacctgg gggccacggc gacgcgggtg ctgctggacg cggtgcagac gggcatcttc 480
gcgggcgacg tggagaagct cagcgtgggc gccacgttcc cgccgctcgt gaagctggag 540
cgcgagcacc gcagcctcct gctcggcgcc atccaggccc agaaggccca gaaggcgcgg 600
gcgaaggccc tgcccgcggg cagcaccgcg ccgaagctga gcggcgcgct gagcaccttc 660
gagggcgggc tcggcacgct catcgacgcc ctgggcacgg cgctcgggga cgcggcgcgc 720
acgggcgcca cggtggaggg cctgacgcgg ggagacgacg gctggcggct cgcggtgagt 780
gagcggggac agcgctcgga gctgaaggcc tcctccgtgg tgctggccgc gcccgcatac 840
gtgacgcggg gcctgttgga gccgctggac gcggagctgg ccgcgcgcgt gggcggcatc 900
gactacgcgc ccatcgccgt ggtgcacctg ggcttcgacg cggggacgac gcccgcgccg 960
gacggcttcg gcttcctggt gccgccgatg gagaagcgcc ggctgctcgg cgccatccac 1020
gcgtccaccg tgttcccctt ccgcgtggag gcgggccggg tgctgtacac gtgcatggtg 1080
ggcggcgcga cgcggccgga cctcgtggcg ctggacgagg ccgaattggt ggccctggcg 1140
cgcgaggagc tgaaggcgct cgcgggcgtc accgccacgc cgacgctcac cgaggtcttc 1200
cgctggaagc ggggcatccc ccagtacaac ctgggccacc tggagcggat ggacggcgtg 1260
gaccgggcgc tcacgcgcct gccagggctg cacctggcgg ggaacgccta caagggcgtg 1320
ggcctcaacg actgcatccg caacgggctg gcgctcgcgg acgcgctggt ggacgcgggg 1380
gcctga 1386
<210> 156
<211> 1386
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 77
<400> 156
atgactgtcg ccgtcgtagg aggtgggatt tcaggtctgg tcgtcgcctg gcgattgcgc 60
tcccgcggta gggatgccgt tctcctggag acaacctccc ggctcggcgg cgcggtgggc 120
acgcgcgcgc agcggggctt cctcctggag acgggcccca acagcttcct ggaccgcgag 180
cccgccatgc gagagctggc atccgcgctc aatctggaac acaggattcg cgcggcggat 240
ggggcggcga agcggcggta tgtctacacg cgggggaagc tgcgctcggt gcccgcatcg 300
ccgccggcgt tcctgaagtc ggacatcctc cccttcggcg cgaagctgcg ggtgatgggg 360
gagctgttca gcggccgcgc ggcgcccggc gtggacgaat ccctggcgga cttcgggcgt 420
cggcacctgg gggccacggc aacgcgggtg ctgctggacg cggtgcagac gggcatcttc 480
gcgggcgacg tggagaagct cagcgtgggc gccacgttcc cgccgctcgt gaagctggag 540
cgcgagcacc gcagcctcct gctcggcgcc atccaggccc agaaggcgca gaaggcacgg 600
gcgaaggccc tgcccgcggg cagcaccggg ccgaagctga gcggcgcgct gagcaccttc 660
gagggcgggc tcggcacgct catcgacgcc ctgggcacgg cgctcgggga cgcggcgcgc 720
acgggcgcca cggtggaggg cctgacgcgg ggagacgacg gctggcggct cgcggtgagt 780
gagcggggac agcgctcgga gctgaaggcc tcctccgtgg tgctggccgc gcccgcatac 840
gtgacgcggg gcctgttgga gccgctggac gcggagctgg ccgcgcgcgt gggcggcatc 900
gactacgcgc ccatcgccgt ggtgcacctg ggcttcgacg cggggacgac gcccgcgccg 960
gacggcttcg gcttcctggt gccgccgatg gagaagcgcc ggctgctcgg cgccatccac 1020
gcgtccaccg tgttcccctt ccgcgtggag gcgggccggg tgctgtacac gtgcatggtg 1080
ggcggcgcga cgcggccgga cctcgtggcg ctggacgagg ccgaattggt ggccctggcg 1140
cgcgaggagc tgaaggcgct cgcgggcgtc accgccacgc cgacgctcac cgaggtcttc 1200
cgctggaagc ggggcatccc ccagtacaac ctgggccacc tggagcggat ggacggcgtg 1260
gaccgggcgc tcacgcgcct gccagggctg cacctggcgg ggaacgccta caagggcgtg 1320
ggcctcaacg actgcatccg caacgggctg gcgctcgcgg acgcgctggt ggacgcgggg 1380
gcctga 1386
<210> 157
<211> 1380
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 78
<400> 157
atgactgtcg ccgtcgtagg aggtgggatt tcaggtctgg tcgtcgcctg gcgattgcgc 60
tcgcggggca aggccgccgt tgtcctggag acaacatccc ggctcggcgg cgcggtgggg 120
actcgcgcga aacaaggttt cctccttgaa acaggtccca acagcttcct ggaccgtgag 180
ccggcgacac gcgagctggc cggcgcgctg aatctggaac acaggattcg cgcggcggat 240
gcggcggcga agcggcgata tgtctacacg cgaggacagc tgcgctcggt gcccgcatcg 300
cccccggcgt tcctgggctc ggacatcctg ccgtggagcg cgaagctgcg ggtcatgggt 360
gagctcttca ccggccgcgc cgcgccgggc atcgatgagt cactggcggc cttcggtcgc 420
agacatctgg gcgccaccgc gacacgcgtg ctgctggacg cggtgcagac aggcatcttc 480
gccggagacg tggagcgctt gagcgtgggc gccaccttcc cgccgctggt gaagctggag 540
cacgagcacc gcagcctcat cctcggcgcc atccggacgc agcaggcccg acgaaaggcc 600
ctgcccgcgg gcgcgtccgc cgcgcccgag ctgagcggcg cgctcagcac gttcgagggc 660
ggcttgcaga cgctcatcga cgcgctgagc gcttcgctgg gggaggacgc gcgcgtgaac 720
gcgacggtgg aagggctgac gcgcgtgggg gacgggtggc ggctcgcggt gagcgagaag 780
ggacagcgct ccgagctgga tgcgtcccac gtggtgctgg ccgcgcccgc atacgtgacg 840
cagaagctct tgcatccgct ggacgcggag ctggcggcgc gcgtgggcgg catcgagtac 900
gcgcccatcg ccgtggtgca gctgggcttc gacgtgggca cgacgcccgc accggatggc 960
ttcgggttcc tggtgcctcc ctcggaggga cggcggctct tgggctccat ccacgcctcc 1020
accgtgttcc ccttccgcgt ggagccgggc cgcgtgctgt acacgtgcat ggtgggcggc 1080
gcgaagcggc cggacctggt gggcttggat gagccggcgc tggtggccct ggcgcgcgag 1140
gagctgaagg cgctcgcggg ggtgacggcc acgccgtcgc tcacggaggt cttccgttgg 1200
cccctgggca tcccccagta caacgtggga catctggcgc ggatggccgc cgtcgaccag 1260
gcgctcaccc gccggcctgg gctctccctc acggggaacg cctacaaggg cgtgggcctc 1320
aacgactgca tccgcaacgg gctccagctc gcggatgcgc tcgtcaccgc ggcggggtga 1380
1380
<210> 158
<211> 1377
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding SEQ ID NO: 79
<400> 158
atgactgtta tcgctgttat cggcggagga atcagcggac tcacgctgac acattgctta 60
cgttctcgcg gcaaggatgc gcttctactg gaagcgagtt ctcggctcgg cggcaacatc 120
gaaacgaggc aacgtgacgg gttcctcatc gagacaggac ccaacagctt cctcgatcgg 180
gagcccgcga cgcgcgagct ggcagcgggc gtcggcgtgg aagatcggat ccgatctgcg 240
gatccggcgg cgaaagcgcg ctacctctac acgcgaggcc gactgcgccc ggtgccatcc 300
tctccgccag cgtttctgaa atcggacatc ctcccgctgg gagcgcgcct gagggtgatg 360
gcggagctct tcacgggccg ggctcccgag ggcgtggacg agtcgctggc agcctttggg 420
cggcggcacc tgggcccggc ggcgacggca gtgctgctgg acgcggtaca gacgggcatc 480
tacgcgggga acatggagac gctgagcgtg gacgccacct tcccgcagct gacgaagctg 540
gagcgcgagc accgcagcct cattctcgga gccatccgct cgcagaaggc gcagcgcaag 600
gcgctccctg cgggggcagc gggaagcccg gagaagctgc gcggcacgct gtgcaccttc 660
gatgggggtc tgcagacgct ggtggatggg ctggcacggg agctcgggcc cgcggcgcac 720
acgaacgcga aggtggaggg tctccagccg agccacggtg gctggcgagt ctccgtgcga 780
gagaacgggg gccaggccga gctgctggca tcccaggtgg tgctggcaac gccagccttc 840
gtggcggcgg ggctgatgcg gccgctggac gagccgctcg cggcgctggt ggagggcatt 900
gcctacgcgc ccatcgccgt ggtgcacctg ggattcgcgc cgggcagcac gcccgcgccg 960
gatggcttcg gcttcctggt gccggggctg gagaagcggc ggctgctggg agccatccac 1020
gcctccacgg tgttcccctt ccgcaccgag ggcggacgcg tcctctacac ctgcatggtg 1080
ggtggagcgc ggcagcccga cctggtgaag ctggacgagg aggcgctggt ggcgctcgcg 1140
cgcgaggagc tcaaggagct ggccggagtg acggcgagcc cgagcttcac ggaggtgatc 1200
cgctggactc ggggcattcc tcagtacaac gtggggcacc tggagcgggt ggccgccatt 1260
gacgcggcgc tgaagcggtg gccggggctg cacctgaccg gcaacgcgta caagggcgtg 1320
ggcatcaacg actgcatccg caacgccttc gcgctgggcg acgcgctggc ggcctga 1377
<210> 159
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 BglII_F primer
<400> 159
gaagatctat gagtgaggta gatgtcg 27
<210> 160
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 SalI_R primer
<400> 160
acgcgtcgac ctagggctgg cctcctga 28
<210> 161
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 BglII_F primer
<400> 161
gaagatctat gatggaggta gatgtcgc 28
<210> 162
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 SalI_R primer
<400> 162
acgcgtcgac ttaacctcct gaaaggtagg c 31
<210> 163
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 BglII_F primer
<400> 163
ccagatctat gattgaggta gatgtcg 27
<210> 164
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 SalI_R primer
<400> 164
ccgtcgacct aggactggcc tcctgc 26
<210> 165
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 XbaI_F primer
<400> 165
agtatctaga atgagtgagg tagatgtcg 29
<210> 166
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 XhoI_R primer
<400> 166
ttaactcgag gggctggcct cctgaaag 28
<210> 167
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 XbaI_F primer
<400> 167
gctctagaat gatggaggta gatgtcgc 28
<210> 168
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 XhoI_R primer
<400> 168
ccgctcgaga cctcctgaaa ggtaggc 27
<210> 169
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 XbaI_F primer
<400> 169
cctctagaat gattgaggta gatgtcg 27
<210> 170
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 XhoI_R primer
<400> 170
ccctcgaggg actggcctcc tgcaaga 27
<210> 171
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 R89A F
<400> 171
gatcgccacc tgccggcgta catctactgg cgg 33
<210> 172
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 R89A R
<400> 172
ccgccagtag atgtacgccg gcaggtggcg atc 33
<210> 173
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C F
<400> 173
gtctctgggt gctatgcggg tgatccacaa caa 33
<210> 174
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C R
<400> 174
acccgcatag cacccagaga caaagggcgc cac 33
<210> 175
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S F
<400> 175
ccctttgtct ctgggagtta tgcgggtgat cca 33
<210> 176
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S R
<400> 176
tggatcaccc gcataactcc cagagacaaa ggg 33
<210> 177
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167C F
<400> 177
gcgccctttg tctctggggt ttattgcggt 30
<210> 178
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167C R
<400> 178
agcactcagt tgttgtggat caccgcaata 30
<210> 179
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167L F
<400> 179
gtctctgggg tttatctggg tgatccacaa caa 33
<210> 180
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167L R
<400> 180
ttgttgtgga tcacccagat aaaccccaga gac 33
<210> 181
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167I F
<400> 181
gtctctgggg tttatatcgg tgatccacaa caa 33
<210> 182
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 A167I R
<400> 182
ttgttgtgga tcaccgatat aaaccccaga gac 33
<210> 183
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V305L F
<400> 183
cattccctat cccaccctag cctgtgtggt cttg 34
<210> 184
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V305L R
<400> 184
caagaccaca caggctaggg tgggataggg aatg 34
<210> 185
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V305M F
<400> 185
tatcccacca tggcctgtgt ggtcttggcc tat 33
<210> 186
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V305M R
<400> 186
cacacaggcc atggtgggat agggaatggt ggccaa 36
<210> 187
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 L327T F
<400> 187
agtgtccgcc ccggctttgg cgtaaccatt 30
<210> 188
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 L327T R
<400> 188
gcggatgccc tgactgcgag gaatggttac 30
<210> 189
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360I F
<400> 189
caagtcttta cgagtattat tggcggtgct ac 32
<210> 190
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360I R
<400> 190
gtagcaccgc caataatact cgtaaagact tg 32
<210> 191
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360L F
<400> 191
gtctttacga gtttaattgg cggtgctacg 30
<210> 192
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360L R
<400> 192
cgtagcaccg ccaattaaac tcgtaaagac 30
<210> 193
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360M F
<400> 193
ggcaagtctt tacgagtatg attggcggtg ctacgg 36
<210> 194
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360M R
<400> 194
ccgtagcacc gccaatcata ctcgtaaaga cttgcc 36
<210> 195
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360V F
<400> 195
caagtcttta cgagtgttat tggcggtgct ac 32
<210> 196
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360V R
<400> 196
gtagcaccgc caataacact cgtaaagact tg 32
<210> 197
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360T F
<400> 197
tttacgagta ctattggcgg tgctacggat cctga 35
<210> 198
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 F360T R
<400> 198
ccgccaatag tactcgtaaa gacttgccaa ccggc 35
<210> 199
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 I408R F
<400> 199
gtttggcgac gggcgagacc ccaatatatg gtg 33
<210> 200
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 I408R R
<400> 200
caccatatat tggggtctcg cccgtcgcca aac 33
<210> 201
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 I408W F
<400> 201
aggtttggcg acgggcgtgg ccccaatata tg 32
<210> 202
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 I408W R
<400> 202
catatattgg ggccacgccc gtcgccaaac ct 32
<210> 203
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167C F
<400> 203
cctttgtctc tgggtgttat tgcggtgatc cacaacaact g 41
<210> 204
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167C R
<400> 204
cagttgttgt ggatcaccgc aataacaccc agagacaaag g 41
<210> 205
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167L F
<400> 205
ggcgcccttt gtctctgggt gctacctggg tgatccacaa ca 42
<210> 206
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167L R
<400> 206
tgttgtggat cacccaggta gcacccagag acaaagggcg cc 42
<210> 207
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167C F
<400> 207
ggcgcccttt gtctctggga gctactgcgg tgatccacaa caac 44
<210> 208
<211> 44
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167C R
<400> 208
gttgttgtgg atcaccgcag tagctcccag agacaaaggg cgcc 44
<210> 209
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167I F
<400> 209
ggcgcccttt gtctctgggt gctacatcgg tgatccaca 39
<210> 210
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165C+A167I R
<400> 210
tgtggatcac cgatgtagca cccagagaca aagggcgcc 39
<210> 211
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167I F
<400> 211
ggcgcccttt gtctctggga gctacatcgg tgatccaca 39
<210> 212
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167I R
<400> 212
tgtggatcac cgatgtagct cccagagaca aagggcgcc 39
<210> 213
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167L F
<400> 213
cctttgtctc tgggtcttat ctgggtgatc cacaacaact g 41
<210> 214
<211> 41
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy19 V165S+A167L R
<400> 214
cagttgttgt ggatcaccca gataagaccc agagacaaag g 41
<210> 215
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 R89A F
<400> 215
gatcgccacc taccggcgta catctactgg cgg 33
<210> 216
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 R89A R
<400> 216
ccgccagtag atgtacgccg gtaggtggcg atc 33
<210> 217
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C F
<400> 217
ccctttgtct ctgggtgtta cgccggtgat ccg 33
<210> 218
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C R
<400> 218
cggatcaccg gcgtaacacc cagagacaaa ggg 33
<210> 219
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S F
<400> 219
ccctttgtct ctgggagtta cgccggtgat ccg 33
<210> 220
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S R
<400> 220
cggatcaccg gcgtaactcc cagagacaaa ggg 33
<210> 221
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167C F
<400> 221
gtctctgggg tttactgtgg tgatccgcaa caa 33
<210> 222
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167C R
<400> 222
ttgttgcgga tcaccacagt aaaccccaga gac 33
<210> 223
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167L F
<400> 223
gtctctgggg tttacctcgg tgatccgcaa caa 33
<210> 224
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167L R
<400> 224
ttgttgcgga tcaccgaggt aaaccccaga gac 33
<210> 225
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167I F
<400> 225
gtctctgggg tttacattgg tgatccgcaa caa 33
<210> 226
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 A167I R
<400> 226
ttgttgcgga tcaccaatgt aaaccccaga gac 33
<210> 227
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V305L F
<400> 227
catcccctat cccaccctag cctgtgtggt cttg 34
<210> 228
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V305L R
<400> 228
caagaccaca caggctaggg tgggataggg gatg 34
<210> 229
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V305M F
<400> 229
atcccctatc ccaccatggc ctgtgtggtc ttg 33
<210> 230
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V305M R
<400> 230
caagaccaca caggccatgg tgggataggg gat 33
<210> 231
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 L327T F
<400> 231
cccggatttg gcgtaacgat tcctcgtggc cag 33
<210> 232
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 L327T R
<400> 232
ctggccacga ggaatcgtta cgccaaatcc ggg 33
<210> 233
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I340T F
<400> 233
cgtacccttg gcaccacatg gtcgtcctgt ctt 33
<210> 234
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I340T R
<400> 234
aagacaggac gaccatgtgg tgccaagggt acg 33
<210> 235
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360I F
<400> 235
caagtcttta caagtaccat tggcggtgcc acg 33
<210> 236
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360I R
<400> 236
cgtggcaccg ccaatggtac ttgtaaagac ttg 33
<210> 237
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360L F
<400> 237
caagtcttta caagtctgat tggcggtgcc acg 33
<210> 238
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360L R
<400> 238
cgtggcaccg ccaatcagac ttgtaaagac ttg 33
<210> 239
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360M F
<400> 239
caagtcttta caagtatgat tggcggtgcc acg 33
<210> 240
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360M R
<400> 240
cgtggcaccg ccaatcatac ttgtaaagac ttg 33
<210> 241
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360V F
<400> 241
caagtcttta caagtgttat tggcggtgcc acg 33
<210> 242
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 F360V R
<400> 242
cgtggcaccg ccaataacac ttgtaaagac ttg 33
<210> 243
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I408R F
<400> 243
gtttggcgac gggcgaggcc ccaatatctt gtg 33
<210> 244
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I408R R
<400> 244
cacaagatat tggggcctcg cccgtcgcca aac 33
<210> 245
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I408W F
<400> 245
gtttggcgac gggcgtggcc ccaatatctt gtg 33
<210> 246
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 I408W R
<400> 246
cacaagatat tggggccacg cccgtcgcca aac 33
<210> 247
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167C F
<400> 247
ccctttgtct ctgggtgtta ctgtggtgat ccgcaacaa 39
<210> 248
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167C R
<400> 248
ttgttgcgga tcaccacagt aacacccaga gacaaaggg 39
<210> 249
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167I F
<400> 249
ccctttgtct ctgggtgtta cattggtgat ccgcaacaa 39
<210> 250
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167I R
<400> 250
ttgttgcgga tcaccaatgt aacacccaga gacaaaggg 39
<210> 251
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167L F
<400> 251
ccctttgtct ctgggtgtta cctcggtgat ccgcaacaa 39
<210> 252
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165C+A167L R
<400> 252
ttgttgcgga tcaccgaggt aacacccaga gacaaaggg 39
<210> 253
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167C F
<400> 253
ccctttgtct ctgggagtta ctgtggtgat ccgcaacaa 39
<210> 254
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167C R
<400> 254
ttgttgcgga tcaccacagt aactcccaga gacaaaggg 39
<210> 255
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167I F
<400> 255
ccctttgtct ctgggagtta cattggtgat ccgcaacaa 39
<210> 256
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167I R
<400> 256
ttgttgcgga tcaccaatgt aactcccaga gacaaaggg 39
<210> 257
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167L F
<400> 257
ccctttgtct ctgggagtta cctcggtgat ccgcaacaa 39
<210> 258
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy20 V165S+A167L R
<400> 258
ttgttgcgga tcaccgaggt aactcccaga gacaaaggg 39
<210> 259
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 R89A F
<400> 259
gatcgccacc taccggcata catctactgg cg 32
<210> 260
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 R89A R
<400> 260
cgccagtaga tgtatgccgg taggtggcga tc 32
<210> 261
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C F
<400> 261
ccctttgtct ctgggtgtta tgccggtgat c 31
<210> 262
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C R
<400> 262
gatcaccggc ataacaccca gagacaaagg g 31
<210> 263
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S F
<400> 263
ccctttgtct ctgggagtta tgccggtgat c 31
<210> 264
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S R
<400> 264
gatcaccggc ataactccca gagacaaagg g 31
<210> 265
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167C F
<400> 265
gtctctgggg tttattgcgg tgatccgcaa c 31
<210> 266
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167C R
<400> 266
gttgcggatc accgcaataa accccagaga c 31
<210> 267
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167L F
<400> 267
gtctctgggg tttatctcgg tgatccgcaa c 31
<210> 268
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167L R
<400> 268
gttgcggatc accgagataa accccagaga c 31
<210> 269
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167I F
<400> 269
gtctctgggg tttatatcgg tgatccgcaa c 31
<210> 270
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 A167I R
<400> 270
gttgcggatc accgatataa accccagaga c 31
<210> 271
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V305L F
<400> 271
catcccctat cccaccctag cctgtgtggt gttg 34
<210> 272
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V305L R
<400> 272
caacaccaca caggctaggg tgggataggg gatg 34
<210> 273
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V305M F
<400> 273
catcccctat cccaccatgg cctgtgtggt gttg 34
<210> 274
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V305M R
<400> 274
caacaccaca caggccatgg tgggataggg gatg 34
<210> 275
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 L327T F
<400> 275
ccggatttgg agtaacggtt cctcgtggtc 30
<210> 276
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 L327T R
<400> 276
gaccacgagg aaccgttact ccaaatccgg 30
<210> 277
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360I F
<400> 277
caagtcttca ccagtattat tggcggtgct ac 32
<210> 278
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360I R
<400> 278
gtagcaccgc caataatact ggtgaagact tg 32
<210> 279
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360L F
<400> 279
gtcttcacca gtttgattgg cggtgctac 29
<210> 280
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360L R
<400> 280
gtagcaccgc caatcaaact ggtgaagac 29
<210> 281
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360M F
<400> 281
gcaagtcttc accagtatga ttggcggtgc tacgg 35
<210> 282
<211> 35
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360M R
<400> 282
ccgtagcacc gccaatcata ctggtgaaga cttgc 35
<210> 283
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360V F
<400> 283
caagtcttca ccagtgttat tggcggtgct ac 32
<210> 284
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 F360V R
<400> 284
gtagcaccgc caataacact ggtgaagact tg 32
<210> 285
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167L F
<400> 285
gccccctttg tctctgggtg ctacctcggt gatccgcaac aa 42
<210> 286
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167L R
<400> 286
ttgttgcgga tcaccgaggt agcacccaga gacaaagggg gc 42
<210> 287
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167C F
<400> 287
gccccctttg tctctgggag ctactgcggt gatccgcaac aa 42
<210> 288
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167C R
<400> 288
ttgttgcgga tcaccgcagt agctcccaga gacaaagggg gc 42
<210> 289
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167C F
<400> 289
ccccctttgt ctctgggtgc tattgcggtg atccgca 37
<210> 290
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167C R
<400> 290
tgcggatcac cgcaatagca cccagagaca aaggggg 37
<210> 291
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167I F
<400> 291
ccccctttgt ctctgggtgc tacatcggtg atccgca 37
<210> 292
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165C+A167I R
<400> 292
tgcggatcac cgatgtagca cccagagaca aaggggg 37
<210> 293
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167I F
<400> 293
ccccctttgt ctctgggagc tacatcggtg atccgca 37
<210> 294
<211> 37
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167I R
<400> 294
tgcggatcac cgatgtagct cccagagaca aaggggg 37
<210> 295
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167L F
<400> 295
gccccctttg tctctgggag ctacctcggt gatccgcaac aa 42
<210> 296
<211> 42
<212> DNA
<213> Artificial Sequence
<220>
<223> Cy18 V165S+A167L R
<400> 296
ttgttgcgga tcaccgaggt agctcccaga gacaaagggg gc 42
<210> 297
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> CyPPO19_XhoI_F
<400> 297
ctcgagatgt ctgaggtgga cgttgcc 27
<210> 298
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> CyPPO19_BamHI_R
<400> 298
ggatccaggt tggcccccgg aaagata 27
<210> 299
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> CyPPO18_XhoI_F
<400> 299
ctcgagatga ttgaagtgga tgtggct 27
<210> 300
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> CyPPO18_BamHI_R
<400> 300
ggatcctgat tgtccaccag cgag 24
<210> 301
<211> 1614
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding AtPPO1 of Arabidopsis thaliana
<400> 301
atggagttat ctcttctccg tccgacgact caatcgcttc ttccgtcgtt ttcgaagccc 60
aatctccgat taaatgttta taagcctctt agactccgtt gttcagtggc cggtggacca 120
accgtcggat cttcaaaaat cgaaggcgga ggaggcacca ccatcacgac ggattgtgtg 180
attgtcggcg gaggtattag tggtctttgc atcgctcagg cgcttgctac taagcatcct 240
gatgctgctc cgaatttaat tgtgaccgag gctaaggatc gtgttggagg caacattatc 300
actcgtgaag agaatggttt tctctgggaa gaaggtccca atagttttca accgtctgat 360
cctatgctca ctatggtggt agatagtggt ttgaaggatg atttggtgtt gggagatcct 420
actgcgccaa ggtttgtgtt gtggaatggg aaattgaggc cggttccatc gaagctaaca 480
gacttaccgt tctttgattt gatgagtatt ggtgggaaga ttagagctgg ttttggtgca 540
cttggcattc gaccgtcacc tccaggtcgt gaagaatctg tggaggagtt tgtacggcgt 600
aacctcggtg atgaggtttt tgagcgcctg attgaaccgt tttgttcagg tgtttatgct 660
ggtgatcctt caaaactgag catgaaagca gcgtttggga aggtttggaa actagagcaa 720
aatggtggaa gcataatagg tggtactttt aaggcaattc aggagaggaa aaacgctccc 780
aaggcagaac gagacccgcg cctgccaaaa ccacagggcc aaacagttgg ttctttcagg 840
aagggacttc gaatgttgcc agaagcaata tctgcaagat taggtagcaa agttaagttg 900
tcttggaagc tctcaggtat cactaagctg gagagcggag gatacaactt aacatatgag 960
actccagatg gtttagtttc cgtgcagagc aaaagtgttg taatgacggt gccatctcat 1020
gttgcaagtg gtctcttgcg ccctctttct gaatctgctg caaatgcact ctcaaaacta 1080
tattacccac cagttgcagc agtatctatc tcgtacccga aagaagcaat ccgaacagaa 1140
tgtttgatag atggtgaact aaagggtttt gggcaattgc atccacgcac gcaaggagtt 1200
gaaacattag gaactatcta cagctcctca ctctttccaa atcgcgcacc gcccggaaga 1260
attttgctgt tgaactacat tggcgggtct acaaacaccg gaattctgtc caagtctgaa 1320
ggtgagttag tggaagcagt tgacagagat ttgaggaaaa tgctaattaa gcctaattcg 1380
accgatccac ttaaattagg agttagggta tggcctcaag ccattcctca gtttctagtt 1440
ggtcactttg atatccttga cacggctaaa tcatctctaa cgtcttcggg ctacgaaggg 1500
ctatttttgg gtggcaatta cgtcgctggt gtagccttag gccggtgtgt agaaggcgca 1560
tatgaaaccg cgattgaggt caacaacttc atgtcacggt acgcttacaa gtaa 1614
<210> 302
<211> 155
<212> DNA
<213> Artificial Sequence
<220>
<223> Gene encoding Transit Peptide of AtPPO1
<400> 302
atggagttat tcttctccgt ccgacgactc aatcgcttct tccgtcgttt tcgaagccca 60
atctccgatt aaatgtttat aagcctctta gactccgttg ttcagtggcc ggtggaccaa 120
ccgtcggatc ttcaaaaatc gaaggcggag gaggc 155
Claims (23)
- 다음에서 선택된 폴리펩타이드:
(1) 서열번호 1의 아미노산 서열을 포함하는 폴리펩타이드;
(2) 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열 중, N59, S60, R89, F161, V165, A167, Q184, P303, V305, F324, L327, I340, F360, 및 I408로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 결실 또는 M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), F(Phe), P(Pro), W(Trp), N(Asn), Q(Gln), G(Gly), Y(Tyr), D(Asp), E(Glu), R(Arg), H(His), 및 K(Lys)로 이루어진 군에서 선택되고, 서열번호 1, 서열번호 2, 또는 서열번호 3의 해당 위치의 원래 아미노산과 상이한 아미노산으로 치환된 아미노산 서열을 포함하는 폴리펩타이드; 및
(3) 상기 (1) 또는 (2)의 폴리펩타이드의 아미노산 서열과 95% 이상의 상동성을 갖는 아미노산 서열로 이루어진 폴리펩타이드. - 제1항에 있어서, 상기 폴리펩타이드는,
(1) 서열번호 1의 아미노산 서열을 포함하는 폴리펩타이드;
(2) 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열 중, R89, V165, A167, V305, L327, F360, 및 I408로 이루어진 군에서 선택된 하나 이상이 각각 독립적으로 M(Met), V(Val), I(Ile), T(Thr), L(Leu), C(Cys), A(Ala), S(Ser), R(Arg), 및 W(Trp)로 이루어진 군에서 선택되고, 서열번호 1, 서열번호 2, 또는 서열번호 3의 해당 위치의 원래 아미노산과 상이한 아미노산으로 치환된 아미노산 서열을 포함하는 폴리펩타이드; 및
(3) 상기 폴리펩타이드 (1) 또는 (2)의 아미노산 서열과 95% 이상의 서열 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드
로 이루어진 군에서 선택된 것인, 폴리펩타이드. - 제1항에 있어서, 상기 폴리펩타이드 (2)는,
(a) 서열번호 1, 서열번호 2, 또는 서열번호 3의 아미노산 서열에 있어서,
(i) F360M, F360V, F360I, F360T, 또는 F360L,
(ii) A167C, A167L, 또는 A167I,
(iii) V305M 또는 V305L,
(iv) R89A,
(v) V165S 또는 V165C,
(vi) L327T, 및
(vii) I408R 또는 I408W
로 이루어진 군에서 선택된 하나 이상의 아미노산 변이를 포함하는 아미노산 서열을 포함하는 폴리펩타이드; 및
(b) 상기 폴리펩타이드의 아미노산 서열과 95% 이상의 상동성을 갖는 아미노산 서열을 포함하는 폴리펩타이드
로 이루어진 군에서 선택된 것인, 폴리펩타이드. - 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드.
- 제5항의 폴리뉴클레오타이드를 포함하는 재조합 벡터.
- 제6항의 재조합 벡터를 포함하는 재조합 세포.
- (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 또는 서열번호 3의 폴리펩타이드 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드 중에서 선택된 하나 이상의 폴리펩타이드;
(2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드;
(3) 상기 (2)의 폴리뉴클레오타이드를 포함하는 재조합 벡터; 및
(4) 상기 (3)의 재조합 벡터를 포함하는 재조합 세포
로 이루어진 군에서 선택된 하나 이상을 포함하는, 식물 또는 조류(algae)의 제초제에 대한 저항성 부여 또는 증진용 조성물. - 제8항에 있어서, 상기 제초제는 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제인, 조성물.
- 제9항에 있어서, 상기 제초제는 피리미딘디온계(pyrimidinediones), 디페닐에테르계(diphenyl-ethers), 페닐피라졸계(phenylpyrazoles), 페닐프탈이미드계(N-phenylphthalimides), 페닐에스테르계 (phenylesters), 티아디아졸계(thiadiazoles), 옥사디아졸계(oxadiazoles), 트리아지논계 (triazinones), 트리아졸리논계(triazolinones), 옥사졸리딘디온계(oxazolidinediones), 피라클로닐(pyraclonil), 플루펜피르-에틸(flufenpyr-ethyl) 및 프로플루아졸(profluazol)로 이루어진 군에서 선택되는 1종 이상인, 조성물.
- 제10항에 있어서, 상기 제초제는 부타페나실(butafenacil), 사플루페나실(saflufenacil), 벤즈펜디존(benzfendizone), 티아페나실(tiafenacil), 포메사펜(fomesafen), 옥시플루오르펜(oxyfluorfen), 아클로니펜(aclonifen), 아시플루오르펜 (acifluorfen), 비페녹스(bifenox), 에톡시펜(ethoxyfen), 락토펜(lactofen), 클로메톡시펜(chlomethoxyfen), 클로르니트로펜 (chlornitrofen), 플루오로글리코펜-에틸(fluoroglycofen-ethyl), 할로사펜(halosafen), 피라플루펜-에틸(pyraflufen-ethyl), 플루아졸레이트(fluazolate), 플루미옥사진 (flumioxazin), 시니돈-에틸(cinidon-ethyl), 플루미클로락-펜틸(flumiclorac-pentyl), 플루티아셋(fluthiacet), 티디아지민(thidiazimin), 옥사디아길(oxadiargyl), 옥사디아존(oxadiazon), 카펜트라존(carfentrazone), 설펜트라존(sulfentrazone), 트리플루디목사진(trifludimoxazin), 아자페니딘(azafenidin), 펜톡사존(pentoxazone), 피라클로닐(pyraclonil), 플루펜피르-에틸(flufenpyr-ethyl), 프로플루아졸(profluazol), 페노필레이트(phenopylate), 페노필레이트의 카바메이트 유사체, 이들의 농약적으로 허용가능한 염으로 이루어진 군에서 선택되는 1종 이상인, 조성물.
- 제8항에 있어서, 상기 식물 또는 조류는 제2의 제초제 저항성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하며 상기 제2의 제초제에 대한 저항성이 부여 또는 증진된 것인, 조성물.
- 제12항에 있어서, 상기 제2의 제초제는 글리포세이트(glyphosate), 글루포시네이트(glufosinate), 디캄바(dicamba), 2,4-D(2,4-dichlorophenoxyacetic acid), 이속사플루톨(isoxaflutole), ALS(acetolactate synthase) 저해성 제초제, 제2광계(photosystem II) 저해성 제초제, 페닐우레아(phenylurea)계 제초제, 브로목시닐(bromoxynil)계 제초제 및 이들의 조합으로 이루어진 군에서 선택되는 것인, 조성물.
- 제12항에 있어서, 상기 제2의 제초제 내성 폴리펩타이드는
글리포세이트(glyphosate) 제초제 내성 EPSPS(glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase), GOX(glyphosate oxidase), GAT (glyphosate-N-acetyltransferase) 또는 글리포세이트 디카복실레이즈(glyphosate decarboxylase);
글루포시네이트(glufosinate) 제초제 내성 PAT(phosphinothricin-N-acetyltransferase);
디캄바(dicamba) 제초제 내성 DMO(dicamba monooxygenase);
2,4-D(2,4-Dichlorophenoxyacetic acid) 제초제 내성 2,4-D 모노옥시게나아제 또는 AAD(aryloxyalkanoate Dioxygenase);
ALS(acetolactate synthase) 저해성 설포닐우레아계 제초제 내성 AHAS(acetohydroxyacid synthase), AHAS(acetohydroxyacid synthase) 또는 At AHASL(Arabidopsis acetohydroxyacid synthase large subnit);
제2광계(photosystem II) 저해성 제초제 내성 광계 II(photosystem II) 단백질 D1;
페닐우레아(phenylurea) 제초제 내성 시토크롬 P450(cytochrome P450);
색소체 저해성 제초제 내성 HPPD(hydroxyphenylpyruvate dioxygenase);
브로목시닐(bromoxynil) 제초제 내성 니트릴레이즈 (nitrilase); 및
이들의 조합으로 이루어진 군에서 선택되는 1종 이상인, 조성물. - 제12항에 있어서, 상기 제2의 제초제 내성 폴리펩타이드를 암호화하는 유전자는
글리포세이트(glyphosate) 제초제 내성 cp4 epsps, epsps(AG), mepsps, 2mepsps, goxv247, gat4601 또는 gat4621 유전자;
글루포시네이트(glufosinate) 제초제 내성 bar 또는 pat 유전자;
디캄바(dicamba) 제초제 내성 dmo 유전자;
2,4-D(2,4-Dichlorophenoxyacetic acid) 제초제 내성 AAD-1 또는 AAD-12 유전자;
이속사플루톨 제초제 내성 HPPDPF W336 유전자;
설포닐 유레아 제초제 내성 ALS, Csr1, Csr1-1, Csr1-2, GM-HRA, S4-HRA, Zm-HRA, SurA 또는 SurB 유전자;
제2광계(photosystem II) 저해성 제초제 내성 psbA 유전자;
페닐우레아(phenylurea) 제초제 내성 CYP76B1 유전자;
브로목시닐(bromoxynil) 제초제 내성 bxn 유전자; 및
이들의 조합으로 이루어진 군에서 선택되는 1종 이상인, 조성물. - (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드, 또는
(2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드
를 포함하는, 식물 또는 조류의 제초제에 대한 내성을 가지는 형질전환체. - 제16항에 있어서, 상기 형질전환체는 조류, 또는 식물의 세포, 원형질체, 캘러스, 클론, 배축, 종자, 자엽, 신초, 식물체 전체, 또는 자손인, 형질전환체.
- (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드, 또는
(2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드를
조류, 또는 식물의 세포, 원형질체, 캘러스, 배축, 종자, 자엽, 신초 또는 식물체 전체에 도입하는 단계를 포함하는,
제초제에 대한 내성을 가지는 식물 또는 조류의 제조 방법. - (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드, 또는
(2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드를
조류, 또는 식물의 원형질체, 캘러스, 배축, 종자, 자엽, 신초 또는 식물체 전체에 도입하는 단계를 포함하는,
식물 또는 조류의 제초제에 대한 내성을 부여 또는 증진시키는 방법. - (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드, 또는 (2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드를 포함하는 식물을 재배지에 제공하는 단계, 및
상기 재배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계를 포함하는,
재배지에서 잡초를 방제하는 방법. - 제20항에 있어서, 상기 재배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계는, 2종 이상의 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 순차적으로 또는 동시에 적용하는 것인, 방법.
- 제20항에 있어서,
상기 식물은 제2의 제초제 내성 폴리펩타이드 또는 이를 암호화하는 유전자를 더욱 포함하는 것이고,
상기 재배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제 및 제2의 제초제의 유효량을 순차적으로 또는 동시에 적용하는 것인, 방법. - (1) 서열번호 1의 폴리펩타이드, 서열번호 2의 폴리펩타이드, 서열번호 3의 폴리펩타이드, 및 제1항 내지 제4항 중 어느 한 항의 폴리펩타이드로 이루어진 군에서 선택된 하나 이상의 폴리펩타이드, 또는 (2) 상기 (1)의 폴리펩타이드를 암호화하는 폴리뉴클레오타이드를 포함하는 조류를 배양 배지에 제공하는 단계, 및
상기 배양 배지에 프로토포르피리노겐 IX 옥시다아제를 억제하는 제초제의 유효량을 적용하는 단계를 포함하는,
배양 배지에서 원하지 않는 수생 생물을 제거하는 방법.
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