KR20200120308A - Mass production method of phytosterol by fermentation treatment of Dendropanax morbifera Liv. leaf - Google Patents

Abstract

The present invention provides a method for massively producing phytosterol by fermentation treatment of Dendropanax morbifera Liv. leaves, which massively produces phytosterol from Dendropanax morbifera Liv. leaves while being differently from commercialization of Dendropanax morbifera Liv. mainly performed in a lignum part. To this end, according to the present invention, the method comprises the following steps: drying collected Dendropanax morbifera Liv. leaves in the shade; pulverizing the Dendropanax morbifera Liv. leaves dried in the shade to acquire Dendropanax morbifera Liv. powder; inputting Dendropanax morbifera Liv. powder in a vessel and adding water to the vessel to mix the same; adding a fermentation agent to the vessel to ferment the Dendropanax morbifera Liv. powder; and drying the fermented Dendropanax morbifera Liv. powder. Accordingly, commercialization of Dendropanax morbifera Liv. is mainly performed in a lignum part until now, but phytosterol can be massively produced from Dendropanax morbifera Liv., and thus forest resources can be efficiently used, a forester can continuously and stably make profits, and a company can stably produce various products due to a massive supplier.

Description

Mass production method of phytosterol by fermentation treatment of Dendropanax morbifera Liv. leaf}

The present invention relates to a method for mass production of phytosterol by fermentation of Hwangchil leaves, and the woody part constitutes the main species in the commercialization of Hwangchil trees so far, but Hwangchil leaf fermentation capable of mass-producing phytosterol (vegetable sterol) from Hwangchil leaves. It relates to a method for mass production of phytosterol by treatment.

The development and industrialization of functional biomaterials from native resources around the world is recognized as a new growth engine, and a lot of investment and research are being made.

Hwangchil tree ( Dendropanax morbifera Liv.) is an evergreen broad-leaved forest of the Araliaceae family. It is a temperate tree grown and cultivated only in the southwestern coast of Jeollanam-do and islands. Since ancient times, it has been used as a natural paint and folk medicine for traditional crafts.

The efficacy of Hwangchil tree is due to the improvement of peripheral vascular circulation, and vegetable mixed sterol (β-sitosterol) is known to exhibit such an effect and is used as a raw material for pharmaceuticals, hypertension, hyperlipidemia, brain infarction, stroke, arthritis, periodontitis, frozen shoulder , Blood circulation, heart disease, tonic effect, skin troubles (acne, atopy), vision recovery, peripheral blood vessels, prostatitis, gout, and immunity are reported to be excellent.

For example, among the sterols found in Hwangchil trees, β-sitosterol is used as a pharmaceutical ingredient as a prostatitis treatment and gum treatment (Insadol).

However, despite various pharmacological effects as a special resource, there is a lack of research results on what is the optimal growth and cultivation environment, physiological and ecological characteristics, and which environment can maximize pharmacological components. .

The following data are background techniques that are helpful in understanding the present invention.

Hwangchil tree extract having anticancer activity (Registration Patent No. 10-0318019, 2001.12.06.registered).

Hwangchil extract, hwangchil fraction, and a pharmaceutical composition containing them (Registration Patent No. 10-0494482, registered on June 1, 2005) having hepatocyte protective effect.

Hwangchil extract that suppresses ethanol-induced liver damage (Public Patent No. 10-2003-0038494, published on June 14, 2003).

Hwangchil extract having a hard tissue regeneration and proliferation effect, hwangchil fraction, and a pharmaceutical composition containing them (Registration Patent No. 10-0457970, 2004.11.10. registration).

Hwangchil extract and hwangchil fraction with skin whitening effect (Publication Patent No. 10-2004-0107853, published on Dec. 23, 2004).

Hwangchil tree seed extract with excellent physiological activity (Registered Patent No. 10-0663284, registered on December 22, 2006).

Manufacturing method of tea bag using Hwangchil (Registration Patent No. 10-0405337, 2003.10.31. Registration).

Manufacturing method of health drink using Hwangchil undiluted solution (Registration Patent No. 10-0441645, 2004.07.14. Registration).

Hwangchil-nyeon tea and its manufacturing method (Registration Patent No. 10-0835868, 2008.05.30. Registration).

Laver or laver containing the Hwangchil wood component and its manufacturing method (Public Patent No. 10-2008-0086745, 2008.09.26. Publication).

Hwangchil sashimi dish with sterilization and detoxification functions (Utility Model Registration No. 20-0432497, 2006.11.28. Registration).

A method for collecting a large amount of Hwangchil and a transplant plate for collecting (Registration Patent No. 10-0199688, 1999.03.05. Registration).

Manufacturing method of synthetic paint containing hwangchil (Registration Patent No. 10-0199689, 1999.03.05. Registration).

Manufacturing method of high-temperature curing natural yellow chil paint for silver products (Registration Patent No. 10-0315096, 2001.11.06 registration).

Method for separating and purifying hwangchil paint and cloves from hwangchil resin liquid (Registration Patent No. 10-0614077, 2006.08.11. Registration).

Separation and purification method of Hwangchil for paint (Registration Patent No. 10-0186682, registered on December 29, 1998).

A lightweight container manufactured using Korean paper and a method for manufacturing the same (Public Patent No. 10-2009-0022271, published on March 4, 2009).

Mixed manufacturing method of hwangchil and lacquer and lacquered lacquer (Registration Patent No. 10-0801583, registered on January 30, 2008).

This research was conducted with the support of the Korea Forest Service (Korea Forestry Promotion Agency) Forest Science and Technology Research and Development Project (FTIS 2016014A00-1919-02).

The problem to be solved of the present invention is to provide a method for mass production of phytosterol by fermentation treatment of hwangchil leaves, which is capable of mass-producing phytosterol from hwangchil leaves, although the woody part is the main species in the commercialization of hwangchil trees so far.

In order to solve the above problems, the present invention provides a step of drying the collected hwangchil leaves in the shade, pulverizing and powdering hwangchil leaves in the shaded state, putting the hwangchil leaf powder in a container and adding moisture to it, and adding a fermenting agent to the container It is characterized in that consisting of the step of fermenting the hwangchil leaf powder by adding and drying the fermented hwangchil leaf powder.

The water addition is characterized in that 55 to 60% by weight of purified water is added to the Hwangchil leaf powder.

The fermenting agent is characterized in that the fallen leaves of Hwangchil trees collected from nature.

The fermentation is characterized in that it takes 7 to 15 days at a temperature condition of 25 to 28 ℃.

And the drying is characterized in that it is made 5 to 10 hours at a temperature condition of 70 ℃.

According to the present invention, the woody part is the main species in the production of Hwangchil trees so far, but as it is possible to produce phytosterol in large quantities from Hwangchil leaves, it is possible to create a sustainable and stable income of the cultivation family as well as efficient use of forest resources. In addition, there is an effect that companies can stably produce a variety of products by having a mass supplier.

1a and b are analysis results of Hwangchil leaf powder fermented according to the present invention.
2a and b are analysis results of hwangchil leaf powder not fermented.
3 is a quality spectrum of peaks detected from Hwangchil leaf powder fermented according to the present invention.
Figure 4 is a photograph of the powder of Hwangchil leaf fermented according to the present invention.

The present invention is to mass-produce phytosterol (vegetable sterol), particularly β-sitosterol, which has already been proven effective as a functional substance of Hwangchil tree as described above, from Hwangchil leaf, hereinafter, by fermentation of Hwangchil leaf according to the present invention. A preferred embodiment of the mass production method of phytosterol will be described in detail.

First, an embodiment according to the present invention

The step of shading the collected hwangchil leaves,

Pulverizing and powdering hwangchil leaves in the shaded state,

Putting Hwangchil leaf powder in a container and adding moisture to it,

Fermenting Hwangchil leaf powder by adding a fermenting agent to the container, and

It consists of drying the fermented hwangchil leaf powder.

In the above technical configuration, in the step of shading the collected hwangchil leaves, hwangchil leaves (hwangchil tree leaves) are collected, foreign substances are removed through washing, etc., and dried in the shade.

Specifically, dry hwangchil leaves for 2-3 weeks in shaded conditions, and dry enough to break when touched.

The step of pulverizing and pulverizing hwangchil leaves in the shaded state is a configuration for aiding fermentation to be described later, and is pulverized to a particle size of 0.02 to 1 mm (preferably 0.5 mm) or less through a pulverizer and powdered.

The step of mixing Hwangchil leaf powder in a container and adding moisture (purified water) is a configuration to aid fermentation, which will be described later, and purified water is added so that water does not flow down when the Hwangchil leaf powder is grasped by hand. Is added so that the moisture is 55-60% by weight based on the weight of the hwangchil leaf powder.

The step of fermenting Hwangchil leaf powder by adding a fermenting agent to the container is a technology composition for mass production of phytosterol from Hwangchil leaves by forming phytosterol (vegetable sterol).The fermenting agent is a microorganism collected and cultured from Hwangchil tree leaves or leaves of Hwangchil tree. It could be itself.

For example, the fallen leaves of Hwangchil trees collected from nature are put in a container, sealed, and then mixed up and down every 2 days under a temperature condition of 25~28℃. After 7~15 days (preferably 11 days), fermentation will occur. Is completed.

In addition, the step of drying the fermented Hwangchil leaf powder is a configuration for increasing storage properties of raw materials for extracting phytosterol, and is performed for 5 to 10 hours at 70°C.

The present invention made up of the above configuration can produce phytosterol in the tissues of hwangchil leaf to produce phytosterol in large quantities from hwangchil leaf.

The following describes the results of analysis of phytosterol content in Hwangchil leaves fermented and unfermented Hwangchil leaves according to the present invention.

First, 1 gram of fermented hwangchil leaf powder and unfermented hwangchil leaf powder were prepared, and each of them was added to 10 ml of methanol to prepare a sample.

Analysis conditions and analysis results by GCMS (QP-2010 Ultra) are as follows.

Phytosterol analysis conditions by GCMS (QP-2010 Ultra) Instrument model GCMS : QP-2010 Ultra (Shimadzu co., Japan) Column : Rtx-5ms
(30 m×0.32 mm ID, 0.25 μm film thickness) Carrier gas : He 1.0 mL/min Injection mode : Split ratio(20:1) Injection temp. : 250℃ Temperature information GC Column temp. : 100℃ Oven temp. program : 40℃(5min) to 200℃(5min) at 2℃/min
to 280℃(10min) at 25℃/min MS Ion source temp. : 210℃ Interface temp. : 250℃ Ionization method : EI Ionization voltage : 70 eV Injection volume : 1.0 mL

1A and B are analysis results of Hwangchil leaf powder fermented according to the present invention, and Figs. 2A and 2B are analysis results of Hwangchil leaf powder not fermented.

Comparing FIGS. 1A and 2B with FIGS. 2A and B, it is shown that there is a large difference in each peak of TIC in the case of fermentation treatment and the case of non-fermentation treatment even in the same hwangchil leaf.

3 is a spectrum of peaks detected from Hwangchil leaf powder fermented according to the present invention, and the production of phytosterol was confirmed from this.

And Figure 4 is a real picture comparing the fermented hwangchil leaf powder, sunny dry hwangchil leaf powder, and shade-dried hwangchil leaf powder according to the present invention.

The above description is an example to aid understanding of the present invention, and does not limit the present invention, and those of ordinary skill in the art to which the present invention pertains may be implemented with various modifications within the scope of matters described in the claims. I will be able to do it.

Claims (5)

The step of shading the collected hwangchil leaves,
Pulverizing and powdering hwangchil leaves in the shaded state,
Putting Hwangchil leaf powder in a container and adding moisture to it,
Fermenting Hwangchil leaf powder by adding a fermenting agent to the container, and
Method for mass production of phytosterol by fermentation treatment of hwangchil leaf, characterized in that consisting of drying the fermented hwangchil leaf powder. The method of claim 1,
The method of mass production of phytosterol by fermentation treatment of hwangchil leaf, characterized in that the moisture is added in an amount of 55 to 60% by weight based on the powder of hwangchil leaf as purified water. The method of claim 1,
The fermenting agent mass production method of phytosterol by fermentation treatment of hwangchil leaves, characterized in that the leaves of Hwangchil tree collected from nature. The method of claim 1,
The fermentation is a mass production method of phytosterol by fermentation treatment of hwangchil leaf, characterized in that the fermentation takes place for 7 to 15 days at a temperature condition of 25 to 28 ℃. The method of claim 1,
The drying is a method for mass production of phytosterol by fermentation treatment of hwangchil leaf, characterized in that the drying is performed for 5 to 10 hours at a temperature of 70°C.

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