KR20200107523A - Pharmaceutical Composition Comprising Extracts of Ginseng and Ginseng Berry for Preventing or Treating Muscle Disease - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases, comprising a mixture of a ginseng extract and a ginseng fruit extract, wherein the mixture of the ginseng extract and the ginseng fruit extract has an effect of alleviating muscle contraction, and thus can be used as an active ingredient in pharmaceuticals, health functional foods, and cosmetic compositions for alleviating muscle diseases caused by muscle mass and muscle loss.

Description

Pharmaceutical Composition Comprising Extracts of Ginseng and Ginseng Berry for Preventing or Treating Muscle Disease comprising ginseng extract and ginseng fruit extract

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases comprising a ginseng extract and a ginseng fruit extract.

Skeletal muscle is an organ that occupies the largest part of the human body and accounts for 40-50% of the total body weight, and plays an important role in various metabolic functions in the body, including energy homeostasis and heat generation. As myoblasts mature into myotubes through the process of myogenesis, the shape of the cells changes at a rapid rate. In the proliferative phase, it is mononuclear, and gradually changes from a circular shape to a spindle shape as the size increases. During the differentiation phase, as the cells become longer and longer, the surrounding cells are fused to each other to differentiate into tube-shaped multinucleated myotube cells.

After the end of differentiation, the root canal cells increase their diameter and length to enlarge the muscle. Muscle differentiation is regulated by myogenic regulatory factors (MRFs) such as MyoD and MRF5, and the expression level of myosin heavy chain (MHC), a major structural protein of root canal cells, is increased in late differentiation. It is known. Therefore, MRF and MHC can be used as markers of muscle differentiation, and the degree of muscle differentiation can be confirmed by measuring their expression levels. And after the end of differentiation, the size of the muscle increases through a process of muscle hypertrophy in which the diameter of the root canal increases and the length of the root canal increases in the root canal cells fused through the protein synthesis pathway.

The physical change in which muscle mass and strength decrease with aging is defined as sarcopenia. Muscle reduction is confirmed by a decrease in the number of canals or diameter of the canals. Human muscles decrease by more than 1% every year from the age of 40, and by the age of 80, the level of maximum muscle mass decreases by 50%, and muscle loss in old age is recognized as the most important factor in reducing overall physical function. This reduction in muscles not only causes daily discomfort, such as changes in body shape and difficulty in maintaining posture, but also lowers basal metabolism, resulting in an imbalance between energy supply/consumption, and ultimately adult diseases including obesity, diabetes, and hyperlipidemia. It causes the occurrence.

Therefore, studies on the cause of sarcopenia and the correlation with the occurrence of adult diseases are being actively conducted.In particular, sarcopenia has a serious impact on not only the occurrence of various metabolic diseases, but also dysfunction, quality of life, and medical costs. Therefore, various efforts are being made to prevent and improve this. As the importance of improving muscle health has emerged, the search for functional materials has been actively conducted.

As a dietary method to prevent sarcopenia in the elderly, it is proposed to consume 25-30 g of high-quality protein at each meal. This is because 4-5 eggs or about 120 g of chicken breast must be consumed at each meal, and it is difficult for ordinary people to practice daily life. Therefore, recently, many people are choosing protein supplements as an alternative, but protein supplements have a high possibility of side effects as they cause excessive protein intake. In addition, if you have kidney disease, you cannot eat a high protein diet, and kidney function decreases with aging. Therefore, alternatives other than high protein intake are needed to prevent sarcopenia. For example, Patent Document 1 discloses that in order to improve sarcopenia, the efficacy of a material derived from a natural product was examined, and it was confirmed that the material contained in cereals and the like has an effect of increasing muscle and muscle strength.

Although many studies are being conducted to develop natural functional materials of plant origin that are safe and economical while having high effectiveness in preventing sarcopenia, development of safe and effective materials that are safe to consume as food is insufficient.

Korean Patent Registration No. 1771680

The present invention is to provide a material for improving muscle reduction derived from a natural product with reduced side effects, and it was confirmed whether it is possible to improve sarcopenia by mixing a natural product material.

In order to solve the above problems, the present invention is a pharmaceutical composition for preventing or treating muscle diseases comprising a ginseng extract and a ginseng fruit extract, a health food composition for improving muscle diseases comprising a ginseng extract and a ginseng fruit extract, and a ginseng extract and It provides a cosmetic composition for improving muscle diseases containing ginseng fruit extract.

When the mixture of the ginseng extract and ginseng fruit extract of the present invention is treated, muscle reduction is improved.

1 shows the diameter of myotube cells when treated with a mixture of red ginseng extract, ginseng fruit extract, red ginseng extract, and ginseng fruit extract to muscle cells caused by muscle contraction.
FIG. 2 shows the weight of gastrocnemius when treated with a mixture of red ginseng extract and ginseng fruit extract in an animal model (skin stapler model) in which muscle contraction is induced.
3 shows the weight of tibialis anterior when treated with a mixture of red ginseng extract and ginseng fruit extract in an animal model (skin stapler model) in which muscle contraction is induced.

Hereinafter, the present invention will be described in detail.

1. Preparation of ginseng extract and ginseng fruit extract

Panax ginseng CA Meyer is a plant of the Araliaceae family. The roots are thin and cylindrical, and sometimes the roots are split into 2-5 in the middle. The length is 5~20cm, and the main root is 0.5~3cm in diameter. The root head is slightly curved, and there is a brain head attached to the stem residue. The bent side is almost flat, light yellowish brown, and the vicinity of the cambium is brown. The roots are mainly used for medicinal purposes, and the fine roots of the roots are removed and dried before use or the whole plant is used. In particular, in Korea, it is classified into white ginseng (unprocessed ginseng), red ginseng (steamed ginseng), and micro ginseng (fine ginseng roots), and is used according to its medicinal efficacy. Ginseng is known to boost vitality and is used for physical weakness, boredom, fatigue, loss of appetite, vomiting, and diarrhea, and to enhance lung function.

In the present invention, the extract of ginseng is a ginseng leaf, young shoot, body, body bark, stem, stem bark, root, root bark, rhizome, flower, stamen group (androecium), pistil group (gynoecium), calyx, It can be obtained from any one selected from the group consisting of stamens, petals, calyx pieces, carpels, and combinations thereof. The stamen group represents all stamens in one flower, and the pistil group represents all pistils in one flower. The carpel is a component that makes pistils of flowers, and generally represents a modified form of leaves, which are collectively referred to as flower leaves. The ginseng may be a root of ginseng, but is not limited thereto, and extracts obtained from other parts of ginseng may be mixed and used for the purpose of preventing or treating muscle diseases.

The types of ginseng roots include fresh ginseng, jiksam, goksam, misam, saenggeon ginseng, red ginseng, taeguksam, and camphor ginseng. There is no limitation on the type of ginseng used in the present invention, but red ginseng may be applied.

The ginseng can be applied to the present invention without limitation as long as it is a plant of the genus Ginseng. For example, Korean ginseng ( Panax ginseng ), Hoegi ginseng ( P. quiquefolius ), Jeonchil ginseng ( P. notoginseng ), Jukjeolsam ( P. japonicus ), Trifolium ginseng ( P. trifolium ), Himalayan ginseng ( P. pseudoginseng ), Vietnam Hemp ( P. vietnamensis ), American ginseng ( P. quinquefolium ), including, but not limited to, any one selected from the group consisting of a combination thereof. Preferably, the ginseng used in the present invention is Korean ginseng ( Panax ginseng ).

The ginseng fruit may be any one selected from the group consisting of ginseng seeds, fruits, unripe fruits, ripe fruits, pulp, pericarp, and a combination thereof, and may preferably be pulp, but is not limited thereto.

The term "extract" as referred to in the present invention refers to a material extracted from a raw material by any method, and is used to include all of the extracted extract, the concentrate obtainable therefrom, the dried product and powder of the concentrate without limitation.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

When the extract is obtained by extracting from the raw material, all conventionally known extraction methods such as solvent extraction method, ultrasonic extraction method, filtration method and reflux extraction method can be used as the extraction method, and it is preferably prepared by using a solvent extraction method or a reflux extraction method. can do. The extraction process may be repeated several times, and thereafter may be additionally subjected to steps such as concentration or freeze-drying. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, the concentrate is freeze-dried, and then a high-concentration extract powder can be prepared using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

The extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. The organic solvent is an alcohol, preferably a C ₁ -C ₄ lower alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, It may be any one selected from the group consisting of benzene and a mixed solvent thereof, and preferably ethanol. When a mixture of water and an organic solvent is used as the extraction solvent, the mixture of water and an organic solvent may preferably be a mixture of water and a lower alcohol of C ₁ -C ₄ , more preferably a mixture of water and ethanol. May be, and even more preferably water.

When extracting ginseng, the amount of solvent added may be 5 to 20 times the weight of the herbal medicine 500 g, preferably 7 to 18 times, more preferably 9 to 16 times, but is not limited thereto. When extracting the ginseng fruit, the amount of solvent added may be 10 to 30 times the weight of the herbal medicine, preferably 13 to 27 times, and more preferably 15 to 25 times, but is not limited thereto. Extraction may be carried out at 60 ℃ to 100 ℃, preferably may be carried out at 65 ℃ to 95 ℃, more preferably may be carried out at 70 ℃ to 90 ℃, but is not limited thereto. Extraction may be carried out for 4 to 20 hours, preferably for 4 to 16 hours, more preferably for 4 to 12 hours, but is not limited thereto. Extraction may be performed 1 to 8 times, preferably 1 to 6 times, more preferably 1 to 5 times, but is not limited to this, the extract obtained from each extraction It may be a single extract or a mixed extract of extracts obtained from each extraction. Among the extraction conditions, when the amount, temperature, and time of the solvent added during extraction are less than the lower limit or the number of extractions exceeds the upper limit, even if the ginseng extract and the ginseng fruit extract are mixed, muscle diseases cannot be improved.

In one embodiment of the present invention, when each extract obtained by extracting red ginseng and ginseng fruit using water as a solvent was mixed and treated, it showed an inhibitory effect on muscle contraction compared to the dexamethasone treatment group, which is a negative control.

The ginseng extract and the ginseng fruit extract may extract a mixture of ginseng and ginseng fruit, or may include a ginseng extract and a ginseng fruit extract, but are not limited thereto.

The extract may be further subjected to a conventional fractionation process in order to increase the effect of improving muscle disease, and fractions obtained through the fractionation process are also included in the present invention. When preparing the fraction, the organic solvent used for preparing the extract may be used. Fractions obtained by passing the extract according to the present invention through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity, or affinity), etc. Active fractions obtained through various purification methods are also included in the fractions of the present invention. By separating the fraction in which several components are mixed according to the properties of the active component through concentration gradient column chromatography, etc., a specific active fraction having a higher muscle disease improvement effect can be prepared.

The column chromatography can separate and purify the active fraction using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl, and XAD resin. . Column chromatography may be performed several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, the elution solvent, the elution rate, and the elution time may be applied to a solvent, speed, or time generally used in the art.

2. Pharmaceutical composition for preventing or improving muscle diseases containing ginseng extract and ginseng fruit extract

The manufacturing method of the ginseng extract and the ginseng fruit extract is the same as described in " 1. Preparation of the ginseng extract and ginseng fruit extract ", so the description is omitted.

The ginseng extract and ginseng fruit extract of the present invention increase the diameter of the root canal cells and restore the muscle of the animal model that caused the muscle loss, so that it can inhibit muscle reduction in muscle diseases caused by muscle function decline, muscle wasting or muscle degeneration. have.

The mixture containing the ginseng extract and the ginseng fruit extract may be a mixture mixed in the following ratio. With respect to 30 parts by weight of the ginseng extract, the ginseng fruit extract may be contained in an amount of 0.1 to 25 parts by weight, preferably the ginseng fruit extract may be included in an amount of 1 to 20 parts by weight, more preferably the ginseng The fruit extract may be included in an amount of 5 to 15 parts by weight, and more preferably, the ginseng fruit extract may be included in an amount of 7 to 13 parts by weight, but is not limited thereto. In addition, with respect to 30 parts by weight of the ginseng extract, the ginseng fruit extract may be included in an amount of 60 to 120 parts by weight, preferably the ginseng fruit extract may be included in an amount of 70 to 110 parts by weight, more preferably The ginseng fruit extract may be included in an amount of 80 to 100 parts by weight, more preferably, the ginseng fruit extract may be included in an amount of 85 to 95 parts by weight, but is not limited thereto. When the ginseng fruit extract is contained below the lower limit, the effect of improving muscle diseases is low even if the ginseng extract and ginseng fruit extract are mixed. In addition, when the ginseng fruit extract exceeds the upper limit, the synergistic effect is lowered even if the ginseng extract and the ginseng fruit extract are mixed.

In one embodiment of the present invention, a mixture of red ginseng extract and ginseng fruit extract was prepared in a ratio of 30:0.1 to 25, 30:30, and 30:60 to 120 and administered to cells or animal models that caused muscle contraction. When the mixture of red ginseng extract and ginseng fruit extract was treated, the root canal diameter increased compared to the negative control group that caused muscle contraction by treatment with dexamethasone, or the muscle of the animal that caused muscle contraction was recovered, thereby inhibiting muscle contraction. . Among the proportions of the mixture, when a mixture containing 60 parts by weight to 120 parts by weight of ginseng fruit extract relative to 30 parts by weight of red ginseng extract was administered to a cell or animal model that caused muscle contraction, the effect of improving muscle contraction was excellent. .

In the present invention, "prevention" refers to any action that suppresses or delays muscle disease by administration of the composition, and "treatment" refers to any action in which muscle disease is improved or beneficially altered by the composition.

The muscle disease refers to a disease caused by a decrease in muscle mass or muscle. Examples include sarcopenia, atony, muscular dystrophy, myasthenia, cachexia, rigid spinesyndrome, and charcot-Marie-Tooth disease. disease) may be any one disease selected from the group consisting of. The hypotonia may be gastric atony, and the muscular dystrophy may be Duchenne muscular dystrophy or myotonic dystrophy, and the muscle atrophy may be post-polio muscle atrophy. atropy), and the cachexia may be cardiac cacakcia, AIDS cacakcia, but is not limited thereto.

In addition to the ginseng extract and ginseng berry extract, the pharmaceutical composition of the present invention is preferably other than the ginseng extract and ginseng berry extract, within a range that does not impair the intended effect of the present invention. Ingredients and the like may be further contained. Conventional adjuvants, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, or carriers may be included, for example.

The route of administration of the pharmaceutical composition includes oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal, such as topical application by application. It can be applied in a way. The parenteral includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount.

In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type and severity of the individual, age, sex, type of disease, It can be determined according to the activity of the drug, its sensitivity to the drug, the time of administration, the route of administration and the rate of excretion, the duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with a conventional therapeutic agent. And can be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and can be easily determined by a person skilled in the art.

The pharmaceutical composition of the present invention is not particularly limited as long as it is an individual for the purpose of improving muscle disease, and any pharmaceutical composition is applicable. For example, non-human animals such as monkeys, dogs, cats, rabbits, mormots, rats, mice, cows, sheep, pigs, goats, etc., humans, birds and fish can be used.

The pharmaceutical composition may further contain one or more active ingredients exhibiting the same or similar function to the ginseng extract and ginseng fruit extract. Each of the above compositions may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method.

Solid preparations for oral administration include powders, granules, tablets, capsules, soft capsules, pills, and the like. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, syrups, etc.In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, and preservatives may be included. have.

Formulations for parenteral administration include powders, granules, tablets, capsules, sterilized aqueous solutions, solutions, non-aqueous solvents, suspensions, emulsions, syrups, suppositories, aerosols, and other external preparations and sterile injectables according to the usual methods. It can be formulated and used in the form of a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta, or cataplasma. However, it is not limited thereto. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, etc. may be used as the non-aqueous solvent and suspension. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The pharmaceutical composition may additionally contain an adjuvant such as a preservative, a stabilizer, a hydrating agent or an emulsification accelerator, a salt and/or a buffering agent for controlling the osmotic pressure, and other therapeutically useful substances. It can be formulated according to the formulation or coating method.

The dosage of the pharmaceutical composition may vary according to various factors including the individual's age, weight, general health, sex, administration time, route of administration, excretion rate, drug formulation, and the severity of a specific disease.

In addition, the pharmaceutical composition of the present invention may be used alone or in combination with surgery, radiation therapy, hormone therapy, chemotherapy, and methods of using a biological response modifier. In addition, the pharmaceutical composition may be administered at a dosage within the range of 0.001 to 200 mg/kg based on an adult, and when the pharmaceutical composition is for external use, once a day in an amount of 1.0 to 3.0 ㎖ based on an adult It is recommended to apply once and continue for at least 1 month, but the dosage is not intended to limit the scope of the present invention.

When formulating the pharmaceutical composition in a unit dosage form, the ginseng extract and ginseng fruit extract of the present invention as active ingredients are preferably contained in a unit dose of about 0.01 to 1,500 mg, and the dosage required for adult treatment is administered. The range of about 1 to 500 mg per day is usually, but not limited to, depending on the frequency and intensity of, and a higher daily dosage may be desirable for some patients.

3. Health food composition for improving muscle diseases containing ginseng extract and ginseng fruit extract

The manufacturing method of the ginseng extract and the ginseng fruit extract is the same as described in " 1. Preparation of the ginseng extract and ginseng fruit extract ", so the description is omitted.

The mixture of the ginseng extract and the ginseng fruit extract is preferably contained in an amount of 0.005 to 20.0% by weight based on the total weight of the health functional food, and more preferably contained in an amount of 0.01 to 10.0% by weight, but is not limited thereto. Does not. When the effective content of the mixture of the ginseng extract and the ginseng fruit extract is less than 0.005% by weight, muscle diseases cannot be alleviated, and when it exceeds 20% by weight, the effect of increasing the efficacy due to the increase in the content decreases, which is uneconomical.

In addition to the mixture of the ginseng extract and the ginseng fruit extract of the present invention, the health functional food has a synergistic effect on the effect of the mixture of the ginseng extract and the ginseng fruit extract within a range that does not impair the intended effect of the present invention. It may further contain other ingredients that can be given. Conventional auxiliaries, such as stabilizers, solubilizers, vitamins, pigments and fragrances, or carriers may be included, for example.

In addition, it may include ingredients that are commonly added during food production, and include, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the carbohydrate include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, a natural flavoring agent taumatin, a stevia extract, and a synthetic flavoring agent may be used. For example, when the health functional food of the present invention is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, natural extract, etc. may be additionally included in addition to the ginseng extract and ginseng fruit extract of the present invention.

There is no particular limitation on the kind of the health functional food. Examples of foods to which the mixture of ginseng extract and ginseng fruit extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, dairy products including gum, ice cream, There are various soups, beverages, teas, drinks, alcoholic beverages, vitamin complexes, and the like, and includes all health functional foods in the usual sense.

4. Cosmetic composition containing ginseng extract and ginseng fruit extract

The manufacturing method of the ginseng extract and the ginseng fruit extract is the same as described in " 1. Preparation of the ginseng extract and ginseng fruit extract ", so the description is omitted.

The cosmetic composition of the present invention may be prepared in a liquid or solid form using bases, auxiliary agents and additives commonly used in the cosmetic field. Cosmetics in a liquid or solid form may include, for example, but not limited to, a form such as a lotion, cream, lotion, and bath agent.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited, and examples include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

When the composition of the present invention is prepared as a cosmetic composition, the composition of the present invention may include not only ginseng extract and ginseng fruit extract, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, Conventional adjuvants such as vitamins, pigments and flavors, and carriers may be included.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl Amidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin moisturizing effect and skin barrier improvement effect according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in detail by way of manufacturing examples and examples.

However, the following Preparation Examples and Examples are only for illustrating the present invention, and the contents of the present invention are not limited by the following Preparation Examples and Examples.

Preparation Example 1. Preparation of red ginseng extract (KGC01pg)

500 g of 6-year-old red ginseng (P. ginseng C.A. Meyer, root 75%, residual rice 25%) was dried at 110°C for 15 minutes using a far-infrared dryer (Korea Energy Technology Co., Ltd., HKD-LAB) and then cut. To 500 g of dried red ginseng, 4 to 8 times of distilled water was added, extracted for 10 hours at 85°C, and the extract was filtered and cooled to 0 to 10°C. Regarding the residue, 4 to 8 times of distilled water was added and extraction was further performed 1 to 4 times for 10 hours. The extracts were filtered and then combined and cooled to 0 to 10°C. The cooled extract is centrifuged at 6,000 rpm for 10 to 20 minutes at 4℃ (Hanil Cymed Co., Ltd., Supra 22K) to remove the sediment, and 50 to 60 using a rotary vacuum concentrator (BUCHI, R-220). It was concentrated to 71° Brix at °C. Finally, sterilized distilled water was added to the concentrate, diluted with 15° Brix, and then freeze-dried (OPERON, FDT-12012) to obtain 230 g of red ginseng concentrate powder.

Preparation Example 2. Preparation of ginseng fruit extract (KGC01bw)

To 1,000 g of dried ginseng fruit flesh ( P. ginseng CA Meyer, Yeoju , Gyeonggi-do), 15 to 25 times of distilled water was added to extract for 6 hours at 85°C, and the extract was filtered and cooled to 0 to 10°C. Regarding the residue, 15 to 25 times of distilled water was added and extraction was further performed 1 to 4 times for 6 hours. The extracts were filtered and then combined and cooled to 0 to 10°C. The cooled extract is centrifuged at 6,000 rpm for 10 to 20 minutes at 4°C (Hanil Cymed Co., Ltd., Supra 22K) to remove the sediment, and 50 to 60 using a rotary vacuum concentrator (BUCHI, R-220). It was concentrated to 60° Brix at ℃. Finally, sterilized distilled water was added to the concentrate, diluted with 15° Brix, and then freeze-dried (OPERON, FDT-12012) to prepare 510 g of ginseng fruit concentrate powder.

Example 1. Confirmation of muscle reduction improvement effect-Confirmation in vitro

C2C12 cells, a myoblast cell line, were cultured in a medium containing 10% FBS (Gibco), 100 units/mL penicillin (Gibco), and 100 mg/mL streptomycin (Gibco). To prepare for the experiment, myoblasts are dispensed into a 12-well plate, and when the cell density is 80-90%, 2% horse serum, 100 units/mL penicillin, and 100 mg/mL streptomycin (Gibco) are included. The culture medium was exchanged to induce cell differentiation. The medium containing horse serum was changed daily for 5-7 days.

When the cells show a rectangular elongated differentiated form, a mixture of red ginseng extract (KGC01pg), ginseng fruit extract (KGC01bw), and red ginseng extract and ginseng fruit extract (KGC01PB, 3:1, 1:1, (Mixed at a ratio of 1:3) were each treated, and dexamethasone (D1756, Sigma, USA), which induces muscle contraction, was treated in each sample treatment group. Red ginseng extract, ginseng fruit extract alone and mixture were diluted in a medium at a concentration of 200 μg/mL and treated, and dexamethasone was treated at a concentration of 100 μM. For comparison, the normal group was not treated with samples or dexamethasone, and the control and experimental groups were treated with dexamethasone to induce muscle contraction. After 24 hours of processing the sample, the shape of the cells was observed under a microscope and photographs were taken.

By measuring the diameter of the cells using the Image J image program, the degree of muscle contraction by dexamethasone and the effect of inhibiting muscle contraction by either red ginseng or ginseng fruit extract alone or a mixture were confirmed. The degree of muscle contraction can be calculated by comparing the dexamethasone-only treatment group with the normal group, and by calculating the muscle thickness of the group treated with dexamethasone and the material compared to the dexamethasone-only treatment group, the muscle reduction recovery rate by the material can be calculated. In the case of the normal group and the control group (dexamethasone treatment group), not the material treatment group, the muscle thickness value corresponding to each group was entered in the'Muscle thickness of the material treatment group' of the following muscle reduction recovery rate calculation method. 100%, and the control has a recovery rate of 0. The recovery rate of muscle reduction is shown in Table 1.

※ How to calculate muscle loss recovery rate

= (Muscle thickness of material treatment group-Muscle thickness of Dexa alone treatment group) / (Muscle thickness of normal group-Muscle thickness of Dexa treatment group)*100

The method of calculating the muscle reduction recovery rate is also used to measure the degree of muscle reduction recovery in vivo of the extracts or mixtures of Examples 2 and 3 below, and in Examples 2 and 3, the calf muscle or calf muscle instead of the muscle thickness in the above calculation method Substitute the weight of the tibialis anterior muscle and obtain the result.

group normal Control
(Dexa) Red ginseng extract alone Ginseng fruit extract alone Red ginseng extract and ginseng fruit extract
Mixture of water 3:1 1:1 1:3 Recovery rate (%) 100 0 73.7 84.4 123.1 100.5 106.5

The control group treated with only dexamethasone reduced the diameter by contracting muscle cells compared to the normal group. A mixture of red ginseng extract, ginseng fruit extract, red ginseng extract, and ginseng fruit extract inhibited muscle cell contraction caused by dexamethasone (see FIG. 1). The mixture of the red ginseng extract and the ginseng fruit extract was superior to the red ginseng extract and the ginseng fruit extract alone in inhibiting the contraction of muscle cells.

From the above results, it was found that red ginseng and ginseng fruit can be used for the purpose of improving muscle reduction, and the effect of improving muscle reduction when mixed rather than single treatment is superior.

Example 2. Confirmation of muscle reduction improvement effect-Confirmation in vivo (dexamethasone animal model)

Sarcopenia is caused by various causes, such as chronic diseases and aging, and is known to be caused even when a drug such as dexamethasone is administered at a high dose. Therefore, dexamethasone was treated in an animal model to create a muscle reduction animal model, and the effect of improving muscle reduction of red ginseng extract, ginseng fruit extract alone or a mixture thereof was verified.

Specifically, mice (C57BL/6N, 7 weeks old, male) were purchased from Orient and subjected to a one-week acclimatization period, and then group separation was performed according to the weight of the mice. Dexamethasone (100mg/kg) was administered intraperitoneally for 2 weeks to induce muscle reduction animal model. After the administration of dexamethasone was stopped after 2 weeks, red ginseng extract (KGC01pg), ginseng fruit extract (KGC01bw) alone or a mixture thereof (KGC01PB, 3:1, 1:3 ratio) was orally administered for 2 weeks. It was used as a control group in which dexamethasone or extract was not administered, and only dexamethasone was administered. After dexamethasone administration, the left hind leg of the experimental group to which the red ginseng extract, ginseng fruit extract alone or a mixture thereof was administered was separated, and the weights of the gastrocnemius and tibialis anterior were measured to confirm the degree of muscle mass recovery. The recovery rate is shown in Table 2 below.

group normal Control
(Dexa) Red ginseng extract alone Ginseng fruit extract alone Red ginseng extract and ginseng fruit extract
Mixture of water 3:1 1:3 Calf
Recovery rate (%) 100 0 17.9 28.5 33.3 45.5 Tibialis anterior muscle
Recovery rate (%) 100 0 35.7 45.2 52.4 97.6

Dexamethasone induced about 20% reduction in muscle mass compared to the normal group. Red ginseng extract and ginseng fruit extract alone increased the amount of muscle recovery, but there was no significant difference. On the other hand, in the case of red ginseng: ginseng fruit mixture (KGC01PB, 1:3), muscle recovered significantly compared to alone. From the above example, it was found through animal model experiments that the muscle recovery effect was excellent when the two were mixed and used compared to the case of using red ginseng and ginseng fruit alone. In particular, the red ginseng: ginseng fruit (1:3) mixture showed better muscle reduction improvement than red ginseng and ginseng fruit alone.

Example 3. Confirmation of muscle reduction improvement effect-confirmation in vivo (skin stapler animal model)

Mice (C57BL/6N, 9 months, male) were purchased from Orient and subjected to a one-week acclimatization period, followed by group separation according to the weight of the mice. Red ginseng extract, ginseng fruit extract, and mixtures thereof were each orally administered at 200 mg/kg/day, and one week later, the hind legs were fixed with a skin stapler (Autosuture Royal 35W Skin Stapler, Tyco Healthcare, QC, Canada). In order to install the skin stapler, the rat was anesthetized, and then the left hind leg was fixed for 2 weeks using a 35W skin stapler to induce muscle reduction. Each extract continued to be administered even after the skin stapler was installed. After completion of the administration for a total of 3 weeks, the left hind limb tibialis anterior and gastrocnemius of normal rats and rats with skin stapler were separated and weighed to confirm the effect of inhibiting muscle reduction of either alone or in combination. I did. Table 3 shows the rate of muscle recovery.

group normal Control
(Dexa) Red ginseng extract alone Ginseng fruit extract alone Red ginseng extract and ginseng fruit extract
Mixture of water (1:3) Calf
Recovery rate (%) 100 0 96.4 98.2 120.5 Tibialis anterior muscle
Recovery rate (%) 100 0 66.7 47.2 197.2

As a result of the experiment, the hind limb fixation using a skin stapler significantly reduced muscle mass compared to the hind limb muscles of the normal group without a stapler. In the case of red ginseng: ginseng fruit mixture (KGC01PB, 1:3), muscle loss was significantly suppressed compared to the control group and alone (see FIGS. 2 and 3).

From the above results, it was found through animal model experiments that, compared to the case of using red ginseng and ginseng fruit alone, when using a mixture of the two, the suppression and recovery effect of muscle loss was excellent. In particular, it was confirmed that the synergistic effect of the red ginseng: ginseng fruit (1:3) mixture was superior to that of red ginseng and ginseng fruit alone.

Preparation Example 3. Preparation of pharmaceutical composition

3-1. Preparation of powder

2 g of a mixture of ginseng extract and ginseng fruit extract of the present invention

1 g lactose

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

3-2. Manufacture of tablets

100 mg of a mixture of ginseng extract and ginseng fruit extract of the present invention

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets were prepared by tableting according to a conventional tablet preparation method.

3-3. Preparation of capsules

100 mg of a mixture of ginseng extract and ginseng fruit extract of the present invention

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, the gelatin capsules were filled according to a conventional capsule preparation method to prepare a capsule formulation.

3-4. Manufacture of pills

1 g of a mixture of ginseng extract and ginseng fruit extract of the present invention

1.5 g lactose

1 g glycerin

0.5 g xylitol

After mixing the above components, it was prepared so as to be 4 g per pill according to a conventional method.

3-5. Preparation of granules

150 mg of a mixture of ginseng extract and ginseng fruit extract of the present invention

Soybean extract 50 mg

200 mg of glucose

Starch 600 mg

After mixing the above ingredients, 100 mg of 30% ethanol was added, dried at 60°C to form granules, and then filled into a cloth.

Manufacture Example 4. Manufacture of health food

4-1. Manufacture of flour food

0.5 to 5.0 parts by weight of a mixture of the ginseng extract and ginseng fruit extract of the present invention was added to flour, and bread, cakes, cookies, crackers, and noodles were prepared using the mixture.

4-2. Preparation of soups and gravies

0.1 to 5.0 parts by weight of a mixture of the ginseng extract and ginseng fruit extract of the present invention was added to soups and broth to prepare health-promoting meat products, noodle soup, and juice.

4-3. Preparation of ground beef

Ground beef for health promotion was prepared by adding 10 parts by weight of a mixture of the ginseng extract and ginseng fruit extract of the present invention to ground beef.

4-4. Manufacture of dairy products

5 to 10 parts by weight of a mixture of the ginseng extract and ginseng fruit extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

4-5. Manufacture of wire

Brown rice, barley, glutinous rice, and adlay were gelatinized by a known method, dried, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

The mixture of the ginseng extract and the ginseng fruit extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air dryer, and the resulting dried product was pulverized with a grinder to a particle size of 60 mesh to obtain a dry powder.

The grains, seeds, and a mixture of the ginseng extract and ginseng fruit extract of the present invention were prepared in the following ratio.

Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley), seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame), a mixture of the ginseng extract and ginseng fruit extract of the present invention ( 3 parts by weight), Ganoderma lucidum (0.5 parts by weight), Rehmannia (0.5 parts by weight)

4-6. Manufacturing of health drinks

Subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%), and 5 g of a mixture of ginseng extract and ginseng fruit extract of the present invention are homogeneously After mixing and instantaneous sterilization, it was prepared by packaging it in a small container such as a glass bottle or a plastic bottle.

4-7. Manufacture of vegetable juice

Vegetable juice was prepared by adding 5 g of a mixture of the ginseng extract and ginseng fruit extract of the present invention to 1,000 ml of tomato or carrot juice.

4-8. Manufacture of fruit juice

Fruit juice was prepared by adding 1 g of a mixture of ginseng extract and ginseng fruit extract of the present invention to 1,000 ml of apple or grape juice.

Preparation Example 5. Preparation of cosmetic formulation

5-1. Preparation of essence

Using a mixture of ginseng extract and ginseng fruit extract, an essence was prepared according to the content (parts by weight) shown in Table 4 below.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 Mixture of ginseng extract and ginseng fruit extract 1.5 Beeswax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Appropriate amount

5-2. Manufacturing of flexible cosmetic water

Softening lotion containing a mixture of ginseng extract and ginseng fruit extract as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDTA 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated Castor Oil 1.00 ethanol 30.00 Mixture of ginseng extract and ginseng fruit extract 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

5-3. Preparation of nourishing cream

A nutritional cream containing a mixture of ginseng extract and ginseng fruit extract as an active ingredient was prepared as shown in Table 6 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitansquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Mixture of ginseng extract and ginseng fruit extract 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Appropriate amount Triethanolamine Appropriate amount Flavoring agent Appropriate amount Purified water Balance

5-4. Lotion manufacture

A lotion containing a mixture of ginseng extract and ginseng fruit extract as an active ingredient was prepared as shown in Table 7 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbital sesquioleate 0.6 Squalene 4.8 Macadamia oil 2 Jojoba Oil 2 Tocopherol acetate 0.4 Methylpolysiloxane 0.2 Tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-pandenol 0.15 Mixture of ginseng extract and ginseng fruit extract 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Appropriate amount

Claims (11)

A pharmaceutical composition for preventing or treating muscle diseases comprising a ginseng extract and a ginseng fruit extract. The method according to claim 1,
With respect to 30 parts by weight of the ginseng extract, the ginseng fruit extract is contained in the range of 0.1 parts by weight to 25 parts by weight, or 60 parts by weight to 120 parts by weight of a pharmaceutical composition. The method according to claim 2,
With respect to the ginseng extract 30 parts by weight, the ginseng fruit extract is a pharmaceutical composition contained in the range of 1 part by weight to 20 parts by weight, or in the range of 70 parts by weight to 110 parts by weight. The method of claim 3,
With respect to the ginseng extract 30 parts by weight, the ginseng fruit extract is a pharmaceutical composition contained in the range of 5 parts by weight to 15 parts by weight, or 80 parts by weight to 100 parts by weight. The method according to claim 1,
The solvent used in the preparation of the extract is any one selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. The method according to claim 1,
The muscle diseases include sarcopenia, atony, muscle dystrophy, myasthenia, cachexia, rigid spinesyndrome, and Charcot-Marie- Tooth disease) is any one selected from the group consisting of a pharmaceutical composition. The method according to claim 1,
The ginseng is ginseng leaves, young shoots, body, body bark, stem, stem bark, root, root bark, rhizome, flower, stamen group (androecium), pistil group (gynoecium), calyx, stamen, petal, calyx piece , A pharmaceutical composition selected from the group consisting of carpels and combinations thereof. The method of claim 7,
The ginseng is a ginseng root, and the ginseng root is any one selected from the group consisting of ginseng, jiksam, goksam, misam, saenggeon ginseng, red ginseng, taegeuk ginseng, camphor ginseng, and combinations thereof. The method of claim 8,
The ginseng is Korean ginseng ( Panax ginseng ), Hoegi ginseng ( P. quiquefolius ), Jeonchil ginseng ( P. notoginseng ), jukjeolsam ( P. japonicus ), trifolium ginseng ( P. trifolium ), Himalayan ginseng ( P. pseudoginseng ), Vietnamese ginseng . ( P. vietnamensis ), American ginseng ( P. quinquefolium ), and any one pharmaceutical composition selected from the group consisting of a combination thereof. Health food composition for improving muscle diseases, including ginseng extract and ginseng fruit extract. A cosmetic composition for improving muscle diseases comprising ginseng extract and ginseng fruit extract.

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