KR102167702B1 - Pharmaceutical Composition Comprising Extracts of Cnidium officinale and Tribulus terrestris for Preventing or Treating Muscle Disease - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases of a mixture of Chunkyung extract and Namgasae extract, and since the mixture of Cnidium extract and Namgasae extract has an effect of improving muscle contraction, the mixture of the present invention is used to reduce muscle mass and muscle It can be used as an active ingredient in pharmaceuticals, health functional foods, and cosmetic compositions to improve muscle diseases caused by.

Description

Pharmaceutical Composition Comprising Extracts of Cnidium officinale and Tribulus terrestris for Preventing or Treating Muscle Disease comprising Cnidium extract and Namgasae extract

The present invention relates to a pharmaceutical composition for preventing or treating muscle diseases comprising a Chunkyul extract and a namgasae extract.

Skeletal muscle is an organ that occupies the largest part of the human body and accounts for 40-50% of the total body weight, and plays an important role in various metabolic functions in the body, including energy homeostasis and heat generation. As myoblasts mature into myotubes through the process of myogenesis, the shape of the cells changes at a rapid rate. In the proliferative phase, it is mononuclear, and gradually changes from a circular shape to a spindle shape as the size increases. During the differentiation phase, as the cells become longer and longer, the surrounding cells are fused to each other to differentiate into tube-shaped multinucleated myotube cells.

After the end of differentiation, the root canal cells increase their diameter and length to enlarge the muscle. Muscle differentiation is regulated by myogenic regulatory factors (MRFs) such as MyoD and MRF5, and the expression level of myosin heavy chain (MHC), a major structural protein of root canal cells, is increased in late differentiation. It is known. Therefore, MRF and MHC can be used as markers of muscle differentiation, and the degree of muscle differentiation can be confirmed by measuring their expression levels. And after the end of differentiation, the size of the muscle increases through a process of muscle hypertrophy in which the diameter of the root canal increases and the length of the root canal increases in the root canal cells fused through the protein synthesis pathway.

The physical change in which muscle mass and strength decrease with aging is defined as sarcopenia. Muscle reduction is confirmed by a decrease in the number of canals or diameter of the canals. Human muscles decrease by more than 1% every year from the age of 40, and by the age of 80, the level of maximum muscle mass decreases by 50%, and muscle loss in old age is recognized as the most important factor in reducing overall physical function. This reduction in muscles not only causes daily discomfort, such as changes in body shape and difficulty in maintaining posture, but also lowers basal metabolism, resulting in an imbalance between energy supply/consumption, and ultimately adult diseases including obesity, diabetes, and hyperlipidemia. It causes the occurrence.

Therefore, studies on the cause of sarcopenia and the correlation with the occurrence of adult diseases are being actively conducted.In particular, sarcopenia has a serious impact on not only the occurrence of various metabolic diseases, but also dysfunction, quality of life, and medical costs. Therefore, various efforts are being made to prevent and improve this. As the importance of improving muscle health has emerged, the search for functional materials has been actively conducted.

As a dietary method to prevent sarcopenia in the elderly, it is proposed to consume 25-30 g of high-quality protein at each meal. This is because 4-5 eggs or about 120 g of chicken breast must be consumed at each meal, and it is difficult for ordinary people to practice daily life. Therefore, recently, many people are choosing protein supplements as an alternative, but protein supplements have a high possibility of side effects as they cause excessive protein intake. In addition, if you have kidney disease, you cannot eat a high protein diet, and kidney function decreases with aging. Therefore, alternatives other than high protein intake are needed to prevent sarcopenia. For example, Patent Document 1 discloses that in order to improve sarcopenia, the efficacy of a material derived from a natural product was examined, and it was confirmed that the material contained in cereals and the like has an effect of increasing muscle and muscle strength.

Although many studies are being conducted to develop natural functional materials of plant origin that are safe and economical while having high effectiveness in preventing sarcopenia, development of safe and effective materials that are safe to consume as food is insufficient.

Korean Patent Registration No. 1771680

The present invention is to provide a material for improving muscle reduction derived from a natural product with reduced side effects, and it was confirmed whether it is possible to improve sarcopenia by mixing a natural product material.

In order to solve the above problems, the present invention is a pharmaceutical composition for preventing or treating muscle diseases, including a cnidium extract and a namgasae extract, a health food composition for improving muscle diseases including a cinnamon extract and a namgasae extract, and a cinnamon extract and a namgasae extract It provides a cosmetic composition for improving muscle diseases comprising a.

There is an effect of improving muscle reduction when treated with a mixture of the Cnidium extract and Namgasae extract of the present invention.

Figure 1 shows the diameter of the root canal cells when treated with a mixture of cnidium extract, namgasae extract, cnidium extract, and namgasae extract to muscle cells caused by muscle contraction.
2 shows the weight of gastrocnemius when treated with a mixture of cnidium extract and namgasae extract in an animal model in which muscle contraction is induced.

Hereinafter, the present invention will be described in detail.

1. Preparation of Cheongung Extract and Namgasae Extract

Cnidium officinale is a perennial herbaceous plant of the umbel family. Flowers bloom in August-September, white, and form a double-lobed inflorescence. There are 5 petals, dried inward, and 5 stamens and 1 pistil. There are 5 to 6 guns and rifles each, in a row shape. The fruit opens but does not mature. Between the nodes in the ground, it looks like a lump with a length of 5 to 10 cm and a diameter of 3 to 5 cm and has a strong fragrance. It is effective in sedation, pain relief, and tonic, so it is used in oriental medicine for headaches and anemia. When it comes to growth, it strengthens the bone marrow and intestines.

Tribulus terrestris is also referred to as a nickname for boredom. The stem is branched from the bottom and splits and grows to the side, reaching about 1m in length. The leaves are 1~6㎝ long, have short stalks, and have small lanceolate triangular leaves. The small leaves are long oval, not the same on both sides, and there are white snow hairs behind the leaves. The place of origin is known as Korea, India, and Taiwan. It is known to be effective in protecting high blood pressure, eye diseases, and liver.

The extract of the present invention is a leaf, young sprout, body, body bark, stem, stem bark, root, root bark, rhizome, seed, fruit, immature fruit, ripe fruit, pulp, pericarp, flower, stamen group (androecium), pistil group (gynoecium), it can be obtained from any one selected from the group consisting of calyx, stamen, petal, calyx piece, carpel, and combinations thereof. The rhizome is also referred to as rhizome, and indicates that the stem grows underground and acts like a root. The stamen group represents all stamens in one flower, and the pistil group represents all pistils in one flower. The carpel is a component that makes pistils of flowers, and generally represents a modified form of leaves, which are collectively referred to as flower leaves. The cnidium may be a rootstock, and the namgasae may be a fruit, but the present invention is not limited thereto, and may be used for the purpose of improving muscle reduction by mixing extracts obtained from other parts of cnidium or namgasae.

The term "extract" as referred to in the present invention refers to a material extracted from a raw material by any method, and is used to include all of the extracted extract, the concentrate obtainable therefrom, the dried product and powder of the concentrate without limitation.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as cultivated or commercially available ones.

When the extract is obtained by extracting from the raw material, all conventionally known extraction methods such as solvent extraction method, ultrasonic extraction method, filtration method and reflux extraction method can be used as the extraction method, and it is preferably prepared by using a solvent extraction method or a reflux extraction method. can do. The extraction process may be repeated several times, and thereafter may be additionally subjected to steps such as concentration or freeze-drying. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrate, the concentrate is freeze-dried, and then a high-concentration extract powder can be prepared using a grinder. The extract also includes a fraction obtained by further fractionating the extract.

The extract may be extracted using water, an organic solvent, or a mixture thereof as an extraction solvent. The organic solvent is an alcohol, preferably a C ₁ -C ₄ lower alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, It may be any one selected from the group consisting of benzene and a mixed solvent thereof, and preferably ethanol. When a mixture of water and an organic solvent is used as an extraction solvent, the mixture of water and an organic solvent may preferably be a mixture of water and a lower alcohol of C ₁ -C ₄ , more preferably a mixture of water and ethanol. I can.

The mixture of water and ethanol may be a 3% (v/v) to 70% (v/v) aqueous ethanol solution, such as 5% (v/v) to 50% (v/v) ethanol aqueous solution, preferably 10% (v/v) to 45% (v/v) ethanol aqueous solution may be, more preferably 15% (v/v) to 40% (v/v) ethanol aqueous solution, even more preferably May be a 20% (v/v) to 35% (v/v) ethanol aqueous solution, but is not limited thereto. When the mixture of water and ethanol is less than the lower limit, muscle diseases cannot be alleviated even if the extract is prepared. When the mixture of water and ethanol exceeds the upper limit, muscle diseases are not improved even if the mixture of Chungoung extract and Namgasae extract is mixed and treated.

In one embodiment of the present invention, when treated with each extract obtained by extracting cnidium with water or a 30% (v/v) aqueous ethanol solution as a solvent, it was confirmed that it exhibited an inhibitory effect on muscle contraction compared to the dexamethasone-treated group as a negative control. Became. Compared to the case of using water as a solvent, the effect of inhibiting muscle contraction was excellent when a 30% (v/v) aqueous ethanol solution was used as the solvent.

When extracting the cnidium, the amount of solvent added may be 5 to 20 times the weight of the herbal medicine 2 kg, preferably 7 to 18 times, and more preferably 9 to 16 times, but is not limited thereto. When extracting namgasae, the amount of solvent added may be 5 to 20 times the weight of the herbal medicine 2 kg, preferably 7 to 18 times, and more preferably 9 to 16 times, but is not limited thereto. Extraction may be carried out at 60 ℃ to 100 ℃, preferably may be carried out at 65 ℃ to 95 ℃, more preferably may be carried out at 70 ℃ to 90 ℃, but is not limited thereto. Extraction may be carried out for 4 to 20 hours, preferably for 4 to 16 hours, more preferably for 4 to 12 hours, but is not limited thereto. Extraction may be performed 1 to 8 times, preferably 1 to 6 times, more preferably 1 to 5 times, but is not limited to this, the extract obtained from each extraction It may be a single extract or a mixed extract of extracts obtained from each extraction. Among the extraction conditions, when the amount, temperature, and time of the solvent added at the time of extraction are less than the lower limit or the number of extractions exceeds the upper limit, the muscle disease cannot be improved even if the cnidium extract and the Namgasae extract are mixed.

The cnidium extract and the namgasae extract may extract a mixture of cnidium and namgasae, or may mix the cnidium extract and the namgasae extract, but are not limited thereto.

The extract may be further subjected to a conventional fractionation process in order to increase the effect of improving muscle disease, and fractions obtained through the fractionation process are also included in the present invention. When preparing the fraction, the organic solvent used for preparing the extract may be used. Fractions obtained by passing the extract according to the present invention through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity, or affinity), etc. Active fractions obtained through various purification methods are also included in the fractions of the present invention. By separating the fraction in which several components are mixed according to the properties of the active component through concentration gradient column chromatography, etc., a specific active fraction having a higher muscle disease improvement effect can be prepared.

The column chromatography can separate and purify the active fraction using a filler selected from the group consisting of silica gel, Sephadex, LH-20, ODS gel, RP-18, polyamide, Toyopearl, and XAD resin. . Column chromatography may be performed several times by selecting an appropriate filler as necessary, but is not limited thereto. In using the chromatography, the elution solvent, the elution rate, and the elution time may be applied to a solvent, speed, or time generally used in the art.

2. A pharmaceutical composition for preventing or improving muscle diseases, including Cnidium extract and Namgasae extract

The method of preparing the Chunkyung extract and Namgasae extract is the same as described in " 1. Preparation of the Chunkyung Extract and Namgasae Extract ", so the description is omitted.

Since the cnidium extract and namgasae extract of the present invention increase the diameter of the root canal cells, it is possible to suppress muscle loss in muscle diseases caused by muscle function decline, muscle wasting or muscle degeneration.

The mixture containing the cnidium extract and the namgasae extract may be a mixture mixed in the following ratio. With respect to 30 parts by weight of the cnidium extract, the namgasae extract may be included in an amount of 0.1 parts by weight to 25 parts by weight, preferably, with respect to 30 parts by weight of the cnidium extract, the namgasae extract may be included in an amount of 1 to 20 parts by weight, , More preferably, with respect to 30 parts by weight of the cnidium extract, the namgasae extract may be included in an amount of 5 parts by weight to 15 parts by weight, and even more preferably, with respect to 30 parts by weight of the cnidium extract, the namgasae extract is 7 parts by weight To 13 parts by weight may be included, but is not limited thereto. When the namgasae extract is included below the lower limit, the effect of improving muscle disease is low even if the mixture of the namgasae extract and the namgasae extract is mixed. In addition, when the namgasae extract is included in excess of the upper limit, there is a problem in that the synergistic effect is lowered even if the cnidium extract and the namgasae extract are mixed.

In one embodiment of the present invention, a mixture of cnidium extract and namgasae extract was prepared in a ratio of 30:0.1 to 25, 30:30, 0.1 to 25:30 and administered to cells or animal models that caused muscle contraction. In the case of treatment with the mixture of Cnidium extract and Namgasae extract, the root canal diameter increased compared to the negative control group that induced muscle contraction by treatment with dexamethasone, or the muscle of the animal in which the muscle contraction was induced was recovered, thereby inhibiting muscle contraction. Among the proportions of the mixture, when a mixture containing 0.1 to 25 parts by weight of namgasae extract was administered to a cell or animal model that caused muscle contraction with respect to 30 parts by weight of Chunkyung extract, the effect of improving muscle contraction was excellent ( 1 and 2).

In the present invention, "prevention" refers to any action that suppresses or delays muscle disease by administration of the composition, and "treatment" refers to any action in which muscle disease is improved or beneficially altered by the composition.

The muscle disease refers to a disease caused by a decrease in muscle mass or muscle. Examples include sarcopenia, atony, muscular dystrophy, myasthenia, cachexia, rigid spinesyndrome, and charcot-Marie-Tooth disease. disease) may be any one disease selected from the group consisting of. The hypotonia may be gastric atony, and the muscular dystrophy may be Duchenne muscular dystrophy or myotonic dystrophy. atropy), and the cachexia may be cardiac cacakcia, AIDS cacakcia, but is not limited thereto.

The pharmaceutical composition of the present invention, in addition to the cinnamon extract and namgasae extract, within a range that does not impair the intended effect of the present invention, preferably other ingredients that can give a synergistic effect to the effect of the cnidium extract and namgasae extract, etc. It may further contain. Conventional adjuvants, such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, or carriers may be included, for example.

The route of administration of the pharmaceutical composition includes oral, intravenous, intramuscular, intraarterial, transdermal, subcutaneous, intraperitoneal, intranasal, intestinal, topical, sublingual or rectal, such as topical application by application. It can be applied in a way. The parenteral includes subcutaneous, intradermal, intravenous, intramuscular, intralesional injection or infusion techniques.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount.

In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type and severity of the individual, age, sex, type of disease, It can be determined according to the activity of the drug, its sensitivity to the drug, the time of administration, the route of administration and the rate of excretion, the duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, and may be administered sequentially or simultaneously with a conventional therapeutic agent. And can be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and can be easily determined by a person skilled in the art.

The pharmaceutical composition of the present invention is not particularly limited as long as it is an individual for the purpose of improving muscle disease, and any pharmaceutical composition is applicable. For example, non-human animals such as monkeys, dogs, cats, rabbits, mormots, rats, mice, cows, sheep, pigs, goats, etc., humans, birds and fish can be used.

The pharmaceutical composition may contain one or more active ingredients exhibiting the same or similar function in addition to the cnidium extract and the namgasae extract. Each of the above compositions may be formulated and used in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, etc., external preparations, suppositories, and sterile injectable solutions according to a conventional method.

Solid preparations for oral administration include powders, granules, tablets, capsules, soft capsules, pills, and the like. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, syrups, etc.In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, and preservatives may be included. have.

Formulations for parenteral administration include powders, granules, tablets, capsules, sterilized aqueous solutions, solutions, non-aqueous solvents, suspensions, emulsions, syrups, suppositories, aerosols, and other external preparations and sterile injectables according to the usual methods. It can be formulated and used in the form of a cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta, or cataplasma. However, it is not limited thereto. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, etc. may be used as the non-aqueous solvent and suspension. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The pharmaceutical composition may additionally contain an adjuvant such as a preservative, a stabilizer, a hydrating agent or an emulsification accelerator, a salt and/or a buffering agent for controlling the osmotic pressure, and other therapeutically useful substances. It can be formulated according to the formulation or coating method.

The dosage of the pharmaceutical composition may vary according to various factors including the individual's age, weight, general health, sex, administration time, route of administration, excretion rate, drug formulation, and the severity of a specific disease.

In addition, the pharmaceutical composition of the present invention may be used alone or in combination with surgery, radiation therapy, hormone therapy, chemotherapy, and methods of using a biological response modifier. In addition, the pharmaceutical composition may be administered at a dosage within the range of 0.001 to 200 mg/kg based on an adult, and when the pharmaceutical composition is for external use, once a day in an amount of 1.0 to 3.0 ㎖ based on an adult It is recommended to apply once and continue for at least 1 month, but the dosage is not intended to limit the scope of the present invention.

When the pharmaceutical composition is formulated in a unit dosage form, as an active ingredient, the Cnidium extract and Namgasae extract of the present invention are preferably contained in a unit dose of about 0.01 to 1,500 mg, and the dosage required for adult treatment is Depending on the frequency and intensity, the range of about 1 to 500 mg per day is usually but not limited thereto, and a higher daily dosage may be desirable for some patients.

3. Health food composition for improving muscle disease, including cnidium extract and namgasae extract

The method of preparing the Chunkyung extract and Namgasae extract is the same as described in " 1. Preparation of the Chunkyung Extract and Namgasae Extract ", so the description is omitted.

The mixture of the cnidium extract and the namgasae extract is preferably contained in an amount of 0.005 to 20.0% by weight based on the total weight of the health functional food, and more preferably contained in an amount of 0.01 to 10.0% by weight, but is not limited thereto. . When the effective content of the mixture of the cnidium extract and the namgasae extract is less than 0.005% by weight, muscle diseases cannot be alleviated, and when it exceeds 20% by weight, the effect of increasing the efficacy due to the increase in the content decreases, which is uneconomical.

The health functional food can give a synergistic effect to the effect of the mixture of the cnidium extract and the namgasae extract, preferably within a range that does not impair the effect of the present invention in addition to the mixture of the cnidium extract and the namgasae extract of the present invention. It may further contain other ingredients and the like. Conventional auxiliaries, such as stabilizers, solubilizers, vitamins, pigments and fragrances, or carriers may be included, for example.

In addition, it may include ingredients that are commonly added during food production, and include, for example, proteins, carbohydrates, fats, nutrients, flavoring agents, and flavoring agents. Examples of the carbohydrate include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, a natural flavoring agent taumatin, a stevia extract, and a synthetic flavoring agent may be used. For example, when the health functional food of the present invention is manufactured as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, natural extracts, etc. may be additionally included in addition to the cnidium extract and namgasae extract of the present invention.

There is no particular limitation on the kind of the health functional food. Examples of foods to which the mixture of the Cnidium extract and Namgasae extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, and various There are soups, beverages, teas, drinks, alcoholic beverages, and vitamin complexes, and all health functional foods in the usual sense are included.

4. Cosmetic composition containing Chunkyung extract and Namgasae extract

The method of preparing the Chunkyung extract and Namgasae extract is the same as described in " 1. Preparation of the Chunkyung Extract and Namgasae Extract ", so the description is omitted.

The cosmetic composition of the present invention may be prepared in a liquid or solid form using bases, auxiliary agents and additives commonly used in the cosmetic field. Cosmetics in a liquid or solid form may include, for example, but not limited to, a form such as a lotion, cream, lotion, and bath agent.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited, and examples include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin, and the like.

When the composition of the present invention is prepared as a cosmetic composition, the composition of the present invention may include components commonly used in cosmetic compositions, as well as cnidium extract and namgasae extract, such as antioxidants, stabilizers, solubilizers, vitamins , Conventional auxiliaries such as pigments and fragrances, and a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

When the formulation of the present invention is a paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethyleneglycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkyl Amidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in duplicate with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having excellent skin moisturizing effect and skin barrier improvement effect according to the present invention may be used according to a conventional method of use, and the number of times of use thereof may be varied according to a user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in detail by way of manufacturing examples and examples.

However, the following Preparation Examples and Examples are only for illustrating the present invention, and the contents of the present invention are not limited by the following Preparation Examples and Examples.

Preparation Example 1. Preparation of water extract of cheonggung

Cnidium officinale (root stem) After adding 10 to 15 times the weight of water to 1 kg, extracting 1 to 3 times for 8 hours at 80°C, the extract obtained by mixing the extracts of each round was filtered using a 5 μm filter. The filtrate is concentrated at 50~60℃ using a rotary decompression concentrator (BUCHI, R-220), and finally diluted to 15°Brix by adding sterilized distilled water to the concentrate and freeze-dried (OPERON, FDT-12012). 304.4 g of water extract powder of Chungoong was obtained.

Preparation Example 2. Preparation of Ethanol Extract (KGC01co)

Extract solution obtained by adding 30% (v/v) ethanol solution of 10 to 15 times the weight to 2 kg of Cnidium officinale (root stem), extracting 1 to 3 times for 8 hours at 80°C, and mixing the extracts of each round Was filtered using a 5 μm filter. The filtrate is concentrated at 50~60℃ using a rotary decompression concentrator (BUCHI, R-220), and finally diluted to 15°Brix by adding sterilized distilled water to the concentrate and freeze-dried (OPERON, FDT-12012). 349.4 g of ethanol extract powder of Chungoong was obtained.

Preparation Example 3. Preparation of Namgasae ethanol extract (KGC01tt)

To 2 kg of namgasae ( Tribulus terrestris L., fruit) , 10 to 15 times the weight of 30% (v/v) ethanol aqueous solution was added, extracted 1 to 3 times for 8 hours at 80℃, and then the extracts of each round were mixed. The extract was filtered using a 5 μm filter. The filtrate is concentrated at 50~60℃ using a rotary decompression concentrator (BUCHI, R-220), and finally diluted to 15°Brix by adding sterilized distilled water to the concentrate and freeze-dried (OPERON, FDT-12012). 268.0 g of ethanol extract powder of Namgasae was obtained.

Example 1. Confirmation of muscle reduction improvement effect-Confirmation in vitro

C2C12 cells, a myoblast cell line, were cultured in a medium containing 10% FBS (Gibco), 100 units/mL penicillin (Gibco), and 100 mg/mL streptomycin (Gibco). To prepare for the experiment, myoblasts are dispensed into a 12-well plate, and when the cell density is 80-90%, 2% horse serum, 100 units/mL penicillin, and 100 mg/mL streptomycin (Gibco) are included. The culture medium was exchanged to induce cell differentiation. The medium containing horse serum was changed daily for 5-7 days.

When the cells show a rectangular elongated differentiated morphology, a mixture of water extract of Chungoong, ethanol extract of Chungung (KGC01co), ethanol extract of Namgasae (KGC01tt), and ethanol extract of Chungoung and Namgasae ethanol extract (KGC01CT) in the sample treatment group, respectively. After treatment, each sample treatment group was treated with dexamethasone (Dexamethasone, D1756, Sigma, USA), which induces muscle contraction. Chungoong, Namgasae alone and the mixture were diluted in a medium at a concentration of 200 μg/mL and treated, and dexamethasone was treated at a concentration of 100 μM. For comparison, the normal group was not treated with samples or dexamethasone, and the control and experimental groups were treated with dexamethasone to induce muscle contraction. After 24 hours of processing the sample, the shape of the cells was observed under a microscope and photographs were taken.

By measuring the diameter of the cells using the Image J image program, the degree of muscle contraction by dexamethasone, and the effect of inhibiting muscle contraction by either cnidium or namgasae extract alone or a mixture were confirmed. The degree of muscle contraction can be calculated by comparing the dexamethasone-only treatment group with the normal group, and by calculating the muscle thickness of the group treated with dexamethasone and the material compared to the dexamethasone-only treatment group, the muscle reduction recovery rate by the material can be calculated. In the case of the normal group and the control group (dexamethasone treatment group), not the material treatment group, the muscle thickness value corresponding to each group was entered in the'Muscle thickness of the material treatment group' of the following muscle reduction recovery rate calculation method. 100%, and the control has a recovery rate of 0.

※ How to calculate muscle loss recovery rate

= (Muscle thickness of material treatment group-Muscle thickness of Dexa alone treatment group) / (Muscle thickness of normal group-Muscle thickness of Dexa treatment group)*100

The method of calculating the muscle reduction recovery rate is also used to measure the degree of muscle reduction recovery in vivo of the extract or mixture of Example 2, and in Example 2, the weight of the calf muscle was substituted in place of the muscle thickness in the above calculation method. Get the result.

1-1. Checking the effect of improving muscle reduction by extraction solvent of Chungoong

It was confirmed whether different solvents during extraction of cnidium had an effect on improvement of muscle reduction. The water extract of cheonggung and ethanol extract of cheonggung prepared in Preparation Examples 1 and 2, respectively, were used as samples. Table 1 shows the muscle reduction recovery rate.

group Normal Control
(Dexa) Cnidium water extract Ethanol Extract Recovery rate (%) 100 0 9.2 66.9

The control group treated with only dexamethasone reduced the diameter by contracting muscle cells compared to the normal group. As shown in Table 1, the water extract of Chunkyung and the ethanol extract of Chunkyung restored muscle reduction, but compared to the extract of water of Chunkyung, the ethanol extract of Chunkyung significantly improved the muscle contraction induced by dexamethasone. It was found that even if cnidium was used for muscle contraction improvement, remarkable muscle contraction improvement effect could be obtained only when ethanol was used as a solvent.

1-2. Confirmation of the effect of improving muscle reduction of the mixture of Chunkyung extract and Namgasae extract

In Example 1-1, the ethanol extract of Chunkyung, which had an excellent effect of improving muscle reduction, was used when preparing a mixture with the extract of Namgasae. The ethanol extract (KGC01co) of Preparation Example 2 and the ethanol extract of Namgasae (KGC01tt) of Preparation Example 3 were mixed in a ratio of 3:1, 1:1, and 1:3, and used as samples (the ethanol extract and the ethanol of Namgasae Mixture of extracts: KGC01CT). The recovery rate of muscle reduction is shown in Table 2.

group normal Control
(Dexa) Ethanol extract alone treatment Treatment of Namgasae ethanol extract alone Mixture of Ethanol Extract and Namgasae Ethanol Extract 3:1 1:1 1:3 Recovery rate (%) 100 0 99.4 55.6 129.4 112.2 105.6

The control group treated with only dexamethasone reduced the diameter by contracting muscle cells compared to the normal group. The mixture of the ethanol extract of Chungoong, the ethanol extract of Namgasae, the ethanol extract of Chungo and the ethanol extract of Namgasae inhibited muscle cell contraction by dexamethasone (see FIG. 1). The mixture of the ethanol extract of Chungoong and the ethanol extract of Namgasae was superior to the ethanol extract of Chungoong and the ethanol extract of Namgasae in inhibiting the contraction of muscle cells. In particular, it was confirmed that the effect of improving muscle reduction was excellent when the ethanol extract of Chungoong and the ethanol extract of Namgasae were mixed at 3:1 compared to the case of mixing at other ratios.

From the above results, it was found that Chungoong and Namgasae can be used for the purpose of improving muscle reduction, and that the effect of improving muscle reduction when mixed is superior to that of single treatment.

Example 2. Confirmation of muscle reduction improvement effect-confirmation in vivo

Mice (C57BL/6N, 7 weeks old, male) were purchased from Orient and subjected to a one week acclimatization period, followed by group separation according to the weight of the mice. Chunkyung ethanol extract, Namgasae ethanol extract, and a mixture thereof were administered orally at 50 mg/kg/day, respectively, and the hind legs were fixed with a skin stapler after 1 week. In order to install the skin stapler, the rat was anesthetized, and then the left hind leg was fixed for 2 weeks using a 35W skin stapler to induce muscle reduction. Each extract continued to be administered even after the skin stapler was installed. After the completion of the administration for a total of 3 weeks, the left hind tibialis anterior and gastrocnemius of the normal rat and the rat with the skin stapler were separated and weighed to confirm the muscle reduction inhibitory effect of either alone or a mixture. Table 3 shows the rate of muscle recovery.

group Normal Control
(Dexa) Ethanol Extract Namgasae ethanol extract Mixture of Ethanol Extract and Namgasae Ethanol Extract (3:1) Recovery rate (%) 100 0 57.1 135.7 157.1

As a result of the experiment, the skin stapler significantly reduced the calf muscle, and a mixture of cnidium ethanol extract, namgasae ethanol extract, cnidium ethanol extract and namgasae ethanol extract inhibited muscle loss (see FIG. 2). In particular, similar to the cell experiment of Example 1-2, it was found that a mixture of 3:1 mixture of Cnidium ethanol extract and Namgasae ethanol extract was effective in inhibiting muscle mass loss.

Preparation Example 4. Preparation of pharmaceutical composition

4-1. Preparation of powder

2 g of a mixture of the Cnidium extract and Namgasae extract of the present invention

1 g lactose

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

4-2. Manufacture of tablets

100 mg of a mixture of the extract of the present invention and the extract of Namgasae

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets were prepared by tableting according to a conventional tablet preparation method.

4-3. Preparation of capsules

100 mg of a mixture of the extract of the present invention and the extract of Namgasae

Corn starch 100 mg

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, the gelatin capsules were filled according to a conventional capsule preparation method to prepare a capsule formulation.

4-4. Manufacture of pills

1 g of a mixture of cnidium extract and namgasae extract of the invention

1.5 g lactose

1 g glycerin

0.5 g xylitol

After mixing the above components, it was prepared so as to be 4 g per pill according to a conventional method.

4-5. Preparation of granules

150 mg of the mixture of the extract of the present invention and the extract of Namgasae

Soybean extract 50 mg

200 mg of glucose

Starch 600 mg

After mixing the above ingredients, 100 mg of 30% ethanol was added, dried at 60°C to form granules, and then filled into a cloth.

Manufacture Example 5. Manufacture of health food

5-1. Manufacture of flour food

0.5 to 5.0 parts by weight of a mixture of the cnidium extract and namgasae extract of the present invention was added to flour, and bread, cakes, cookies, crackers, and noodles were prepared using the mixture.

5-2. Preparation of soups and gravies

0.1 to 5.0 parts by weight of a mixture of the Cnidium extract and Namgasae extract of the present invention were added to soups and broth to prepare health-promoting meat products, noodle soup, and broth.

5-3. Preparation of ground beef

Ground beef for health promotion was prepared by adding 10 parts by weight of a mixture of the Cnidium extract and Namgasae extract of the present invention to the ground beef.

5-4. Manufacture of dairy products

5 to 10 parts by weight of a mixture of the cnidium extract and namgasae extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

5-5. Manufacture of wire

Brown rice, barley, glutinous rice, and adlay were gelatinized by a known method, dried, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into powder having a particle size of 60 mesh with a grinder.

The mixture of the extract of Chunkyung and Namgasae of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by spraying and drying with a hot air dryer was pulverized with a grinder to a particle size of 60 mesh to obtain a dry powder.

The grains, seeds, and a mixture of the cnidium extract and the namgasae extract of the present invention were prepared in the following ratio.

Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley), seeds (7 parts by weight of perilla, 8 parts by weight of black soybeans, 7 parts by weight of black sesame), a mixture of cnidium extract of the present invention and Namgasae extract (3 Parts by weight), Ganoderma lucidum (0.5 parts by weight), Rehmannia (0.5 parts by weight)

5-6. Manufacturing of health drinks

Homogeneously blended subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), and water (75%), and 5 g of a mixture of cnidium extract and Namgasae extract of the present invention Then, after instantaneous sterilization, it was prepared by packaging it in a small container such as a glass bottle or a plastic bottle.

5-7. Manufacture of vegetable juice

Vegetable juice was prepared by adding 5 g of a mixture of the extract of Chunkyung and Namgasae of the present invention to 1,000 ml of tomato or carrot juice.

5-8. Manufacture of fruit juice

A fruit juice was prepared by adding 1 g of a mixture of the Cnidium extract and Namgasae extract of the present invention to 1,000 ml of apple or grape juice.

Preparation Example 6. Preparation of cosmetic formulation

6-1. Preparation of essence

Essence was prepared according to the content (parts by weight) shown in Table 4 below by using a mixture of Chunkyung extract and Namgasae extract.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 Mixture of Chunkyung extract and Namgasae extract 1.5 Beeswax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Appropriate amount

6-2. Manufacturing of flexible cosmetic water

Flexible cosmetic water containing a mixture of Chunkyung extract and Namgasae extract as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDTA 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated Castor Oil 1.00 ethanol 30.00 Mixture of Chunkyung extract and Namgasae extract 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

6-3. Preparation of nourishing cream

A nutrient cream containing a mixture of Chunkyung extract and Namgasae extract as an active ingredient was prepared as shown in Table 6 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic Acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitansquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Mixture of Chunkyung extract and Namgasae extract 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Appropriate amount Triethanolamine Appropriate amount Flavoring agent Appropriate amount Purified water Balance

6-4. Lotion manufacture

A lotion containing a mixture of Chunkyung extract and Namgasae extract as an active ingredient was prepared as shown in Table 7 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbital sesquioleate 0.6 Squalene 4.8 Macadamia oil 2 Jojoba Oil 2 Tocopherol acetate 0.4 Methylpolysiloxane 0.2 Tocopherol acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-pandenol 0.15 Mixture of Chunkyung extract and Namgasae extract 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Appropriate amount

Claims (8)

As a pharmaceutical composition for the prevention or treatment of muscle diseases comprising a cnidium extract and a namgasae extract,
The muscle diseases include sarcopenia, atony, muscular dystrophy, myasthenia, cach exia, rigid spinesyndrome, and Charcot-Marie- Tooth disease) of any one selected from the group consisting of a pharmaceutical composition. The method according to claim 1,
With respect to 30 parts by weight of the cnidium extract, the namgasae extract is a pharmaceutical composition containing 0.1 parts by weight to 25 parts by weight. The method according to claim 2,
A pharmaceutical composition containing 1 to 20 parts by weight of the namgasae extract, based on 30 parts by weight of the cnidium extract. The method of claim 3,
With respect to 30 parts by weight of the cnidium extract, the namgasae extract is a pharmaceutical composition comprising 5 parts by weight to 15 parts by weight. The method according to claim 1,
The solvent used in the preparation of the extract is any one selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof. delete As a health food composition for improving muscle disease, comprising a cnidium extract and a namgasae extract,
The muscle diseases include sarcopenia, atony, muscular dystrophy, myasthenia, cach exia, rigid spinesyndrome, and Charcot-Marie- Tooth disease) any one selected from the group consisting of health food composition. As a cosmetic composition for improving muscle diseases comprising a Chunkyung extract and Namgasae extract,
The muscle diseases include sarcopenia, atony, muscular dystrophy, myasthenia, cach exia, rigid spinesyndrome, and Charcot-Marie- Tooth disease) any one selected from the group consisting of a cosmetic composition.

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