KR20200102384A - Cosmetic Composition for UV and Blue Light Protection Comprising Extract of Carbonized Dendropanax morbifera - Google Patents

Abstract

The present invention provides a cosmetic composition for blocking ultraviolet rays and blue light, comprising a carbonized Dendropanax morbiferus extract as an active ingredient. The Dendropanax morbiferus extract of the present invention exhibits an excellent UV/blue light shielding effect by absorption and scattering by dispersed carbon nanoparticles. In addition, the Dendropanax morbiferus extract of the present invention has a high content of antioxidants such as flavonoids and phenolic compounds. Accordingly, the Dendropanax morbiferus extract of the present invention, as a sunscreen, can be used for a cosmetic material that exhibits an effect of preventing skin aging as well.

Description

A cosmetic composition for UV and Blue Light Protection Comprising Extract of Carbonized Dendropanax morbifera containing carbonized Hwangchil extract

The present invention relates to a cosmetic composition using hwangchil.

The sun's rays are composed of ultraviolet (5%), visible (39%), and infrared (56%). Among them, UV rays, which have a short wavelength and have a lot of energy, have been pointed out as the cause of skin cancer and skin aging, and so far, attention has been focused only on UV protection. However, recently, reports on adverse effects of high-energy visible light (HEV light) other than the ultraviolet region on the human body and skin have been followed. In particular, the visible light in the blue light (400-500 nm) region, which is known to come from electronic devices such as LED lamps, smartphones, and computers, has higher energy than visible light of other wavelengths and is frequently exposed in daily life. There is a growing concern about the impact on it. Looking at the effects of blue light on the skin in addition to increasing eye fatigue, it is said that it generates free radicals and free radicals and promotes photoaging such as carotenoid decomposition, mitochondrial DNA damage, fibroblast viability decrease, pigmentation induction. Many research reports have been made.

Sunscreens are divided into organic sunscreens that absorb UV light and inorganic sunscreens that block the penetration of ultraviolet rays into the skin mainly by physical scattering rather than absorbing light. Various substances such as benzophenone, p-amino benzoic acid (PABA), and octhyl methoxy cinnamate (OMC) are used as organic sunscreens, and titanium dioxide (TiO ₂ ) and zinc oxide (ZnO) are used as inorganic sunscreens.

Since the organic sunscreen is dissolved in the formulation and is widely applied to the skin, it is possible to create a cosmetic formulation that has excellent UV protection effect even with a small amount and has a good feeling of use without clouding. However, organic sunscreens are synthesized chemicals that can penetrate the skin and cause contact dermatitis, and chemical changes are caused by UV rays, which may cause secondary problems. On the other hand, inorganic sunscreens, which are mineral substances, are difficult to disperse, making it difficult to make a formulation, and when the amount of formulation increases, the formulation becomes opaque, causes a cloudiness, and deteriorates the feeling of use, but does not have side effects such as contact dermatitis and the blocking effect lasts a long time. Commercially available sunscreens mostly use both organic sunscreens and inorganic sunscreens to compensate for their respective strengths and weaknesses, but recently, for the sake of skin safety, we are trying to reduce organic sunscreens and increase the specific gravity of inorganic sunscreens.

Titanium dioxide, which is most widely used as an inorganic sunscreen, has a large refractive index, so it scatters light well. The smaller the particle size is, the more excellent the UV-B blocking effect is. However, when exposed to light for a long time, active oxygen is produced by photocatalytic activity, which can cause decomposition of organic substances and phototoxicity problems. Zinc oxide, which has less UV-blocking ability than titanium dioxide, mainly blocks UV-A, so it is widely used in combination with titanium dioxide.Nano-sized zinc oxide also exhibits photocatalytic activity when exposed to UV light, and when dissolved in acidic or basic aqueous solutions Poorly soluble salts may be formed by reacting with fatty acids and anionic components in oil. Titanium dioxide or zinc oxide has a UV blocking effect, but the blocking effect is very weak for light with a wavelength of 400 nm or more such as blue light.

In order to absorb ultraviolet rays with a wavelength of 400 nm or less, it must have a band gap energy of about 3 eV. In addition to titanium dioxide and zinc oxide, cerium oxide is another mineral. Cerium oxide can block harmful light in a wide area from ultraviolet light to blue light, and is relatively safe for skin because it has low photocatalytic activity. Therefore, cerium oxide is very likely as an alternative material that overcomes the limitations of titanium dioxide, but it is not yet widely used in cosmetic formulations as a sunscreen. Since cerium oxide is a nano-sized powder, when simply added to a cosmetic formulation, aggregation occurs and dispersion in water is difficult. In addition, it can penetrate pores, and when used in a solid powder formulation, it has a problem of lumpy and stiff feeling.

On the other hand, Hwangchil extract has the effect of inhibiting the production of MMP-1, the effect of inhibiting the production of MMP-1 by UV induction, the effect of promoting collagen synthesis, the effect of inhibiting the expression of inflammatory cytokines by UV irradiation, and the effect of improving wrinkles, so it is used as a cosmetic. It is known that it can be (Korean Patent Registration No. 10-1176529). However, there has been no attempt to develop a cosmetic for blocking ultraviolet rays and blue light using hwangchil.

In this situation, the present inventors provided a sunscreen cosmetic composition comprising the carbonized extract of Hwangchil as an active ingredient, which confirmed that it has high UV and blue light blocking effects and at the same time has an antioxidant effect. Completed.

One technical problem to be achieved by the present invention is to provide a cosmetic composition for blocking ultraviolet rays and blue light, comprising an extract of Hwangchil treated with carbonization as an active ingredient.

Another technical problem to be achieved by the present invention is a first step of carbonizing hwangchil; And it is to provide a method for producing a hwangchil extract for blocking ultraviolet rays and blue light comprising a second step of extracting the carbonized hwangchil.

The technical problem to be achieved by the present invention is not limited to the technical problems mentioned above, and other technical problems that are not mentioned can be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. There will be.

Hereinafter, the present invention will be described in more detail.

On the other hand, each description and embodiment disclosed herein can be applied to each other description and embodiment. That is, all combinations of various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be said that the scope of the present invention is limited by the specific description described below.

In addition, those of ordinary skill in the art can recognize or ascertain using only routine experimentation a number of equivalents to the specific aspects of the invention described in this application. Also, such equivalents are intended to be included in the present invention.

As an aspect for solving the above problems, the present invention provides a cosmetic composition for blocking ultraviolet rays and blue light, comprising an extract of Hwangchil treated with carbonization as an active ingredient.

The raw material used in the extract of the present invention, Hwangchil ( Dendropanax morbiferus ) refers to the Hwangchil tree, which is a tall tree with blue leaves throughout the four seasons. The Hwangchil tree reaches 15m in height, and the young branches are green and hairless. Leaves are alternate and egg-shaped or oval. Also, the edge of the leaves is flat, but in young trees, it is divided into 3~5 and there are saw teeth. Flowers bloom in light yellow-green in June, are bisexual, and hang on the umbel. The stalk is 3~5cm long and the stalk is 5~10mm long. The calyx is divided into 5 pieces, the petals and stamens are 5 pieces each, and there is a honey gland on the flowerbed. The pistil head is divided into 5 pieces, and the drupe is oval, and it matures to black in October, and the style is left. Yellow liquid comes out when the bark is scratched, which is called Hwangchil and was used as a paint for furniture. It is a Korean endemic species and grows in the south.

In the present invention, the extract is obtained by carbonization and extraction using various organs or parts (eg, leaves, flowers, roots, stems, branches, shells, seeds, etc.) of Hwangchil as a raw material, and preferably leaves , Stem, branch, bark or root is carbonized and extracted, more preferably leaves, branches, bark or root are carbonized and extracted, and most preferably leaves and branches are carbonized and extracted.

According to one embodiment of the present application, hwangchil as the raw material may be pulverized. Specifically, the average size of the pulverized particles may be 5.0 mm or less. If the particle size is larger than this, the generation of carbon nanoparticles may be weak during carbonization.

The extract of the present invention is obtained by extracting the carbonized hwangchil. As used herein, the term “carbonization treatment” means providing a physical or chemical stimulus to an organic precursor to form carbon particles. Herein, the carbonization treatment may be performed by any one selected from ultrasonic irradiation, hydrothermal method, and microwave method, but may be used without limitation as long as it is a method of carbonizing an organic material precursor.

Specifically, the ultrasonic (ultrasound irradiation) treatment may be performed by processing ultrasonic waves of 1 to 10 kHz at 60 to 100°C for 30 to 180 minutes. The hydrothermal treatment may be performed by treating at 100 to 500°C for 10 to 180 minutes. The microwave treatment may be performed for 8 to 12 minutes in a 700 to 900 W microwave oven. Specifically, it may be performed in a micro oven of 750 to 850 W for 9 to 11 minutes. When carbonization is performed under conditions stronger than the above conditions or for a long time, the content of antioxidant components may be reduced, and when carbonization is performed under conditions weaker than the above conditions or for a short period of time, the generation of carbon nanoparticles suitable for UV protection may decrease.

According to the exemplary embodiment of the present disclosure, the carbonization treatment may be performed while the raw material hwangchil is immersed in a solvent. As the solvent, water, ethanol, aqueous ammonia, etc. may be used, and water may be preferably used. According to the exemplary embodiment of the present disclosure, the carbonization treatment may be performed while immersing 1 part by weight of raw material hwangchil in 5 to 10 parts by weight of a solvent. When immersed in more solvents, an appropriate level of carbonization may not occur, and when immersed in less than this, carbonization proceeds excessively and antioxidant components may be destroyed.

Further, according to the exemplary embodiment of the present disclosure, the carbonization treatment may be performed in a state in which the raw material hwangchil is mixed with a water-soluble amine compound. Since the Hwangchil carbon nanoparticles provided according to the extraction method of the present invention exhibit anionic properties, stable composite particles can be made when the raw material Hwangchil is mixed with a cationic water-soluble amine compound and then carbonized. It can show high absorption of blue light. The water-soluble amine compound is generally used in the art and is not particularly limited. Specifically, it may be any one or more selected from the group consisting of alkanol amines, polyalkylene polyamines, aliphatic amines, aromatic amines, cyclic amines, aminoclays, and chitosan. For example, hydroxylamine, monoethanolamine, diethanolamine, triethanolamine, 2-(2-aminoethoxy)ethanol, N,N-dimethylethylamine, N,N-diethylethanolamine, N,N -Alkanol amines such as dibutyl ethanolamine, N-methylethanolamine, N-ethylethanolamine, N-butylethanolamine, N-methyldiethanolamine, monoisopropanolamine, and triisopropanolamine; Diethylenetriamine, triethylenetetraamine, propylenediamine, N,N-diethylethylenediamine, N,N'-diethylethylenediamine, 1,4-butanediamine, N-ethyl-ethylenediamine, 1,2propane Polyalkylene polyamines such as diamine, 1,3-propanediamine, and 1,6-hexanediamide; Aliphatic amines such as hydrazine, 2-ethyl-hexylamine, dioctylamine, tributylamine, tripropylamine, heptylamine, and cyclohexylamine; Aromatic amines such as morpholine, triazine, triazole, porphyrin, benzylamine, and diphenylamine; Cyclic amines such as piperidine, N-methyl-piperadine, methyl-piperadine, and hydroxylethylpiperidine; Aminoclays; Chitosan, etc. are mentioned.

According to the exemplary embodiment of the present disclosure, filtration treatment may be performed as necessary to remove contaminants such as pollutants, soluble and odorous substances, or heavy metals prior to carbonization. The filtration step may be performed according to a conventional method, and in particular, may be performed by a reduced pressure filtration method using filter paper. In addition, after the vacuum filtration, if necessary, a membrane filtration method using a membrane filter using a membrane as a filter medium may be further performed.

The extract of the present invention is obtained by extracting the carbonized hwangchil. The extraction method is not limited and may be used as long as it is an extraction method performed in the art. In the present invention, preferably, a solvent extraction method using an extraction solvent was used. Hwangchil extract in the present invention is a variety of extraction solvents, for example, water, anhydrous or hydrated lower alcohols having 1-4 carbon atoms (eg, methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol , Glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane, and can be obtained using one or more extraction solvents selected from the group consisting of mixtures thereof, preferably water, C _{1 -6 It} can be obtained by using alcohol, or a solvent that is a mixture thereof, and more preferably can be obtained by using water. The extract may be provided in a liquid form obtained by immersing in the extraction solvent for a long time. According to one embodiment of the present application, the extraction solvent may be used in an amount of 5 to 10 mL per 1 g of raw material hwangchil.

According to one embodiment of the present application, the extraction may be performed for 2 to 4 hours, and when extracted for longer than this, the extract may contain an excessive amount of unnecessary components, and when extracted less than this, the extract may contain a sufficient amount of flavonoids and phenols. Sex compounds, and may not contain carbon nanoparticles.

According to the exemplary embodiment of the present application, the extract may be provided through several additional processes commonly used in the art, such as centrifugation, drying, and filtration, if necessary.

As the hwangchil extract of the present invention contains carbon nanoparticles, it can be used as a cosmetic for blocking ultraviolet rays and blue light. The sunscreen cosmetic composition herein may be used not only for ultraviolet rays, but also for blue light blocking.

In connection, light blocking by certain particles is achieved by absorption and scattering. Mainly, inorganic powder particles have a greater effect of blocking light by scattering than absorbing light. Hwangchil extract of the present invention not only absorbs light in the ultraviolet region, but also has an effect of blocking light in two regions by scattering the light in the blue light region. According to an experimental example of the present application, it was confirmed that the Hwangchil extract of the present invention does not transmit ultraviolet rays, but also does not transmit blue light and scatters (Experimental Examples 1, 2, and 3).

The term "carbon nanoparticles" as used herein is a term used interchangeably with carbon nano quantum dots and carbon nanodots, and refers to spherical particles composed of carbon atoms. The carbon nanoparticles are not present in the raw material Hwangchil, but may be produced by carbonization. The carbon nanoparticles may have an average particle size of 1 nm to 10 nm, and preferably may have an average particle size of 1 nm to 5 nm. If the particle size is smaller or larger than this, the effect of scattering and/or reflecting light for blocking ultraviolet rays may be reduced.

According to one embodiment of the present application, the cosmetic composition may include a component of Hwangchil in an amount of 7.5 to 30mg/mL. When included in a lower content, the effect of improving UV protection may be slightly insignificant, and in the case of a higher content, irritation may be exhibited. According to an experimental example of the present invention, it was confirmed that ultraviolet rays and blue light can be blocked when the concentration is displayed.

On the other hand, since the extract of the present invention may contain additional active ingredients such as flavonoids or phenolic compounds in a high content, it may be used not only for UV protection, but also for antioxidant, anti-aging, and improvement of atopic dermatitis. According to an experimental example of the present invention, it was confirmed that the extract of the present invention contained flavonoids or phenolic compounds in a high content, and thus did not exhibit cytotoxicity.

According to the exemplary embodiment of the present application, the cosmetic composition may be provided by additionally including a silane compound. The term “silane compound” as used herein refers to a compound in which four substituents are bonded to a central silicon atom. The silane compound of the present invention is one in which at least one hydrocarbon chain-containing group and at least one hydrolyzable group are bonded to a central silicon atom. As the silane compound is further included, a complex between a cation and an anion may be formed, or a composition in a gel form may be formed by a sol-gel method. In this case, a stable solid network structure can be formed, and the cosmetic composition can improve blue light absorption ability. According to an experimental example of the present application, a mixture of a silane compound such as APTES and a hwangchil extract forms a complex between an anion and a cation and partially forms a stable phase by a sol-gel reaction, so that the absorption of blue light light is significantly increased. It can be seen that it can be confirmed (Experimental Example 3).

According to the exemplary embodiment of the present disclosure, the cosmetic composition may contain 5 to 15% by weight of a silane compound relative to the total weight.

According to the exemplary embodiment of the present disclosure, the cosmetic composition may further include other inorganic sunscreens or organic sunscreens used in the art. Accordingly, UV blocking and blue light blocking effects may be enhanced. In particular, since the inorganic sunscreen has a strong property of scattering ultraviolet rays, when used together with the Hwangchil extract of the present invention, which has a strong ultraviolet-absorbing property, the ultraviolet-blocking effect may be remarkably exhibited.

On the other hand, organic sunscreens have the potential to be absorbed by the skin and detected in the blood, so recently developed cosmetics tend to refrain from adding organic sunscreens. As an alternative to cosmetics containing both an organic sunscreen and an inorganic sunscreen, the cosmetics containing the hwangchil extract and the inorganic sunscreen of the present invention exhibit excellent effects by absorption and scattering of UV/blue light, while solving the toxicity problem. There is an advantage. You can expect the whitening and wrinkle improvement effects of Hwangchil extract itself.

According to the exemplary embodiment of the present disclosure, the cosmetic composition may contain 0.1 to 1.0% by weight of an inorganic sunscreen based on the total weight.

In one embodiment of the present application, the inorganic sunscreen agent may be selected from the group consisting of cerium oxide, titanium dioxide (TiO ₂ ) and zinc oxide (ZnO), cerium aminoclay (CeAC), and combinations thereof.

In one embodiment of the present application, the organic sunscreen is octyl methoxycinnamate, ethylhexylsalicylate, homosalate, 4-methylbenzylidene camphor ), Drometrizole, Drometrizole Siloxane, Digaloyl Trioleate, Disodium Phenyl Dibenzimidazole Tetrasulfonate Acid, D Ethylamino Hydroxybenzoyl Hexyl Benzoate, Diethylamino Hydroxybenzoyl Hexyl Benzoate, Diethylhexyl Butamido Triazone, Methylenebis-Benzotria Zolyl tetramethylbutylphenol (Methylene Bis-Benzotriazolyl Tetramethylbutylphenol), Menthyl Anthranilate, Benzophenone-3, Benzophenone-4, Benzophenone-8 ), Butyl Methoxydibenzoylmethane, Bis-EthylhexyloxyphenolMethoxyphenyl Triazine, Cinoxate, Ethylhexyl Dimethyl PABA, Ethylhexylmethoxycinna Mate (Ethylhexyl Methoxycinnamate), Ethylhexyl Triazone, Octocrylene, Isoamyl p-Methoxycinnamate, Terephthalylidene Dicamphor Sulfonic Acid, Phenylbenzimidazole Sulfonic Acid, Polysilicone-15, and combinations thereof It can be.

According to an experimental example of the present application, it was confirmed that a mixture of an inorganic sunscreen such as cationic CeAC (cerium-aminoclay) and a hwangchil extract exhibits an increased UV-blocking effect by light scattering (Experimental Example 3).

According to one embodiment of the present application, the cosmetic composition is formulated as one of the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, oils, powder foundations, emulsion foundations, and sprays. However, it is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, a nutrition lotion, a nutrition cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack or a powder.

In addition, the ingredients included in the cosmetic composition may include ingredients commonly used in cosmetic compositions, in addition to the above ingredients. For example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers Can include.

As another aspect for solving the above problems, the present invention comprises a first step of carbonizing hwangchil; And it provides a method for producing a hwangchil extract for blocking ultraviolet rays and blue light comprising a second step of extracting the carbonized hwangchil. The terms and methods used herein are as described above.

Hwangchil extract of the present invention exhibits excellent UV/blue light shielding effect by absorption and scattering by the dispersed carbon nanoparticles. In addition, the hwangchil extract of the present invention has a high content of antioxidants such as flavonoids and phenolic compounds. Accordingly, the hwangchil extract of the present invention can be used as a sunscreen in a cosmetic that simultaneously exhibits an effect of preventing skin aging.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a diagram showing the manufacturing process of Example 1.
2 to 4 are diagrams showing blue light transmission test results for Examples 1 to 3, respectively.
5 to 7 are diagrams showing fluorescence pattern experiment results for Examples 1, 2, and 3.
8 is a diagram showing a result of measuring the absorbance of ultraviolet-visible light for Examples 1 to 3;
9 is a diagram showing the result of measuring the absorbance of ultraviolet-visible light for Example 2 compared to Example 1. FIG.
10 is a diagram showing the result of measuring the absorbance of ultraviolet-visible light for Example 3 compared to Example 1. FIG.
11 is a diagram showing the results according to the cytotoxicity evaluation of Example 1.
12 is a diagram showing a calibration curve graph for quantification of a phenolic compound.
13 is a diagram showing a result of measuring absorbance for quantification of a phenolic compound.
14 is a diagram showing a calibration curve graph for quantification of flavonoids.
15 is a diagram showing a result of measuring absorbance for quantification of flavonoids.

Hereinafter, the present invention will be described in more detail by the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1- Carbonization treatment water extract of Hwangchil

3 g of hwangchil (leaf and branch) and 30 mL of distilled water (DI water) were mixed and pulverized using a grinder. The pulverized particle size was 0.5 mm to 5.0 mm. This was placed in a microwave oven and heated by irradiating an 800 W microwave for 10 minutes (carbonization treatment). Accordingly, a carbide was obtained, and the obtained carbide was immersed in 20 ml of distilled water and left at room temperature for 3 hours. After that, the centrifugation was repeated to take the supernatant to prepare Example 1. The concentration of the extract of Example 1 was confirmed to be 30mg/mL. The manufacturing process of Example 1 is shown in FIG. 1.

Comparative Example 1-Non-carbonized black extract of Hwangchil (Black Extract 5%)

After obtaining the pulverized hwangchil particles in the same manner as in Example 1, a black extract was obtained by bathing in 100°C water.

Comparative Example 2-Non-carbonized transparent extract of Hwangchil (Transparent Extract 5%)

After obtaining the pulverized hwangchil particles in the same manner as in Example 1, distillation extraction was performed using distilled water at 100°C to obtain a liquid extract through a cooling machine.

Example 2- Mixture of carbonized extract of Hwangchil and CeAC

Example 2 was prepared by mixing CeAC (cerium-aminoclay) to a 30mg/mL concentration of hwangchil extract according to Example 1 at a predetermined concentration. The cerium-aminoclay was prepared using cerium chloride hydrate (CeCl3·7H2O), 3-Aminopropyltriethoxysilane (hereinafter'APTES') and alcohol as precursors, and Patent No. 10-1968307 CeAC in powder form was obtained according to the method described in Example 1.

Example 3-Mixture of carbonized extract of Hwangchil and APTES

Example 3 was prepared by mixing 3-aminopropyltriethoxysilane (APTES) with a 30 mg/mL concentration of hwangchil extract according to Example 1 at a predetermined concentration. In the mixture, anionic carbon nanoparticles and cationic APTES formed a stable complex, and a sol-gel reaction partially appeared to form a stable phase.

Experimental Example 1. Measurement of blue light transmission to hwangchil extract

The hwangchil extract of Example 1 was diluted to a concentration of 3.75, 7.5, 15, and 30 mg/mL to transmit blue light of 430 to 450 nm using a black light UV lamp (ROBUST UV 12LED). Water was used as a control.

The experimental results are shown in FIG. 2. In the case of Example 1 at the concentrations of 30mg/mL, 15mg/mL, and 7.5mg/mL, blue light could not be transmitted, and blue light was faintly transmitted in the sample of 3.75 mg/mL.

The same experiment was carried out for Examples 2 and 3. Fig. 3 shows the results of performing the above experiment by diluting 1, 2, and 4 times for Example 2 in which CeAC was mixed at 0.6% by weight of Hwangchil extract of 30mg/mL. The results of performing the above experiment were diluted 1 times, 2 times, 4 times, 8 times, 16 times, and 32 times for Example 3 in which 10% by weight of APTES was mixed with the extract of Example 1 at 30 mg/mL. It is shown in Figure 4.

Example 2 prevented the transmission of UV, and when diluted 2 times and 4 times, it was found that the light was faintly transmitted. In Example 3, it was found that the transmission of light was prevented until 16 times of dilution, and light was faintly transmitted even when diluted about 32 times.

As such, Example 1 was found to have blue light shielding ability, and Examples 2 and 3 in which CeAC or APTES were mixed were also found to have blue light shielding ability.

Experimental Example 2. Fluorescence experiment according to excitation by wavelength of Hwangchil extract

It was intended to confirm the fluorescence of the light identified in Experimental Example 1. For Examples 1, 2, and 3, light in the blue light region having a wavelength of 320 to 460 nm was irradiated, and then the spectrum of fluorescent light was investigated. Specifically, light of short wavelengths of 320nm, 340nm, 360nm, 380nm, 400nm, 420nm, 440nm, and 460nm was excited to perform in Examples 1, 2, and 3, and fluorescence spectra were measured. Here, in Examples 2 and 3, 0.6% by weight of CeAC was mixed with the Hwangchil extract at 30 mg/mL and APTES at 10% by weight was mixed with the extract of Example 1 at 30 mg/mL.

Results of fluorescence experiments for Examples 1, 2, and 3 are shown in FIGS. 5 to 7. When irradiating (excitation) light in the blue light region, it was found that fluorescence was emitted as light with a longer wavelength of 450 nm or more. In other words, since the emitted light emits light of a long wavelength instead of blue light, skin can be protected. Accordingly, it was found that Examples 1 to 3 had a skin protective effect against the light of blue light.

Experimental Example 3. Measurement of absorbance of Hwangchil extract when irradiated with light

It was attempted to confirm whether the shielding effect confirmed in Experimental Example 1 was due to absorption of light. For Example 1, Example 2 containing 0.5% by weight of CeAC, and Example 3 containing 15% by weight of APTES, the absorbance of Hwangchil extract was measured when light was irradiated . The absorbance according to the wavelength is shown in FIG. 8.

In addition, compared to Example 1, the absorbance measurement results for Example 2 containing 0.2, 0.4, 0.6, and 0.8% by weight of CeAC are shown in FIG. 9.

In addition, as compared with Example 1, the absorbance measurement results for Example 3 containing 10, 20, and 40 wt% APTES are shown in FIG. 10.

As a result, it was confirmed that Examples 1 and 2 absorb light in the ultraviolet region of about 380 nm or less, and Example 3 was found to absorb light of about 480 nm or less.

When the results of Experimental Examples 1 to 3 are summarized, it was found that the hwangchil extract of the present invention can be used as an excellent UV blocker by absorbing and scattering light in the ultraviolet and blue light regions.

In particular, it was found that Example 2, in which Hwangchil extract and CeAc were mixed, exhibited excellent blocking effect through blocking by scattering or reflection rather than blocking by absorption of ultraviolet/blue light.

In addition, Example 3 in which Hwangchil extract and APTES were mixed showed increased absorption of blue light.

As described above, by further including an additional sunscreen agent or excipient, it is possible to improve absorption, scattering, and blocking of ultraviolet rays or blue light by reflection.

Experimental Example 4. Cytotoxicity evaluation (MTT Assay)

To confirm the efficacy of the 　HaCaT 　 keratin cell survival rate of the sunscreen cosmetic composition of the present invention 　HaCaT 　 keratin cell viability analysis experiment was performed.

The HaCaT keratinocytes were cultured in DMEM/F-12 medium containing 10% fetal bovine serum, 100U/ml penicillin, 0.1mg/ml streptomycin, 0.25g/ml amphotericin B, and 10g/ml hydrocortisone. HaCaT keratinocytes were cultured at 37°C in a humid atmosphere containing 5% CO ₂ and 95% air.

In the HaCaT 　 keratinocyte viability analysis experiment, 　 HaCaT keratin cells were treated with the above Example 1 (Black Extract) at a constant concentration for 24 hours and 48 hours at a constant concentration, and the cell viability was determined through the MTT method and expressed as a control percentage. That is, 20 µl of a 5mg/ml solution of 3-(4,5-dime thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was added to the treated product and incubated in a 37°C 5% CO₂ incubator for 2 hours. Then, when the cells were dissolved by adding 0.1N acidified CH3Cl-HCl, the cell viability was determined by measuring the absorbance at 590nm with an ELISA plate reader.

The absorbance is shown in Table 1, and the cell viability accordingly is shown in Table 2 and Fig. 11.

Concentration
(μg/mL) Absorbance at 590 nm 24 hours 48 hours 0 1.135±0.055 1.561±0.059 10 1.141±0.086 1.481±0.101 50 1.099±0.194 1.433±0.071 100 1.076±0.164 1.285±0.048 200 1.064±0.021 1.202±0.041 300 1.008±0.044 1.254±0.010

Concentration
(μg/mL) Cell viability (%) 24 hours 48 hours 0 99.97±0.37 99.98±3.77 10 100.53±4.93 94.88±3.75 50 96.86±2.25 91.80±2.61 100 94.84±3.58 82.35±2.35 300 93.74±1.83 76.97±2.60 500 88.81±0.371 80.32±0.64

As such, it was found that Example 1, a carbonized water extract of Hwangchil, had low toxicity to HaCaT cells under appropriate conditions (0-300 μg/mL). It is believed that this is due to having antioxidant properties.

Experimental Example 5. Quantification of phenolic compounds

The total content of phenolic compounds among the main components contained in the hwangchil extract of Example 1, Comparative Example 1 and Comparative Example 2 was measured by modifying the Gutfinger method.

Specifically, 2% (w/v) Na ₂ CO ₃ was added to Example 1, Comparative Example 1, and Comparative Example 2, 2 mL was added, and left for 2 minutes, and 0.2 mL of 50% Folin-Ciocalteu reagent was added, and then at room temperature. After standing for 30 minutes, absorbance was measured at 750 nm. A calibration curve for quantification of phenolic compounds in the sample was prepared using gallic acid at a concentration of 50 to 500 μg/mL. The calibration curve was formed as a graph of y= 0.02992+0.00112x (R ² =0.9969) (Fig. 12). The absorbance measurement results are shown in FIG. 13 and Table 3.

Concentration Absorbance GA 50 μg/mL 0.0281 GA 100 μg/mL 0.0758 GA 200 μg/mL 0.1946 GA 400 μg/mL 0.4346 GA 500 μg/mL 0.5189 Comparative Example 1 (Black extract 5%) 0.3207 Comparative Example 2 (Transparent extract 5%) 0.0043 Example 1 (0.2 g/mL) 0.2526

Through the above results, it was found that the phenolic compound was contained in an amount of 252.25 μg/mL in Example 1, 313.03 μg/mL in Comparative Example 1, and 30.57 μg/mL in Comparative Example 2. That is, it was found that the extract of Example 1 had a very high phenolic compound content compared to the content of the raw material hwangchil.

Experimental Example 6. Quantification of flavonoid compounds

The total content of flavonoid compounds among the main components included in Example 1, Comparative Example 1, and Comparative Example 2 was measured by the method of Moreno et al.

Specifically, 0.1 mL of the extract for each concentration was taken, mixed with 4.3 mL of 80% ethanol containing 10% aluminum nitrate and 1M potassium acetate, and allowed to stand at room temperature for 40 minutes, and then 415 nm. Absorbance was measured at. At this time, the amount of total flavonoids was calculated from a calibration curve prepared using quercetin (Sigma Chemical Co.) to obtain the content. The calibration curve was formed as a graph of y=0.005+0.0298x (R ² =0.9841) (Fig. 14). The absorbance measurement results are shown in FIG. 15 and Table 4.

Concentration Absorbance Quercetin 5 μg/mL 0.1714 Quercetin 10 μg/mL 0.3599 Quercetin 15 μg/mL 0.5457 Quercetin 30 μg/mL 1.0018 Quercetin 40 μg/mL 1.2665 Quercetin 50 μg/mL 1.4711 Comparative Example 1 (Black extract 5%) 1.0117 Comparative Example 2 (Transparent extract 5%) 0.0053 Example 1 (0.2 g/mL) 0.6769

Through the above results, it was found that flavonoids were contained in an amount of 21.04 μg/mL in Example 1, 32.37 μg/mL in Comparative Example 1, and 0 μg/mL in Comparative Example 2. That is, it was found that the extract of Example 1 had a very high flavonoid content compared to the content of the raw material hwangchil.

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (9)

A cosmetic composition for blocking ultraviolet rays and blue light, comprising a carbonized extract of Hwangchil as an active ingredient.
The cosmetic composition according to claim 1, wherein the carbonization treatment is performed while immersing 1 part by weight of the raw material hwangchil in 5 to 10 parts by weight of a solvent.
The cosmetic composition of claim 1, wherein the carbonization treatment is performed by any one selected from ultrasonic irradiation, hydrothermal method, and microwave method.
The cosmetic composition of claim 1, wherein the carbonization treatment is performed by irradiating a microwave of 700 to 900 W for 8 to 12 minutes.
The cosmetic composition of claim 1, wherein the extract is extracted for 2 to 4 hours by immersing the carbonized hwangchil in water, C _1-6 alcohol, or a solvent such as a mixture thereof.
The cosmetic composition of claim 1, wherein the cosmetic composition comprises the carbonized hwangchil in an amount of 7.5 to 30 mg/mL.
The cosmetic composition of claim 1, wherein the cosmetic composition further comprises a silane compound.
The cosmetic composition of claim 1, wherein the cosmetic composition further comprises an inorganic sunscreen.
A first step of carbonizing hwangchil; And comprising a second step of extracting the carbonized hwangchil, UV and blue light blocking method for producing hwangchil extract.

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