KR20190078715A - 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 - Google Patents
암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 Download PDFInfo
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Abstract
본 발명은 암 세포의 유전체 DNA의 변이와 항암제의 약물 반응을 예측하는 유전자 변이를 검출하는데 사용하기 위한 조성물, 상기 조성물을 포함하는 차세대 염기 서열 분석(NGS) 기반 혼합 진단 패널, 및 상기 패널을 이용하여 상기 유전자 변이를 진단 및 검출하는 방법에 관한 것이다.
본 발명의 암 세포의 유전체 DNA의 변이 및 약물 반응 유전자의 변이를 검출 및 진단하기 위한 방법은 주요 암 관련 유전자 및 약물 대사유전자의 다양한 유전자 변이를 단 한번의 실험을 통해 검출할 수 있어 각각의 유전체 변이마다 다른 플랫폼을 사용하는 종래 방법보다 매우 경제적이고 효율적이다. 또한 암의 유발, 진행 및 약물 대사 관련된 주요 유전자를 대상으로 높은 커버리지 (high coverage)로 시퀀싱함으로써 고해상도를 확보하여 기존 방법으로 검출이 되지 않았던 낮은 빈도 유전체 변이의 동정이 가능하다. 따라서, 본 발명의 방법을 이용하면 암 세포의 유전체 DNA의 변이와 약물 반응을 예측하는 유전자 변이를 포함하는 시료에서 다양한 유전자 변이를 동시에 높은 민감도와 정확도로 분석할 수 있고, 이러한 분석 결과를 바탕으로 환자 맞춤형 암 치료제를 효율적으로 탐색할 수 있다.
본 발명의 암 세포의 유전체 DNA의 변이 및 약물 반응 유전자의 변이를 검출 및 진단하기 위한 방법은 주요 암 관련 유전자 및 약물 대사유전자의 다양한 유전자 변이를 단 한번의 실험을 통해 검출할 수 있어 각각의 유전체 변이마다 다른 플랫폼을 사용하는 종래 방법보다 매우 경제적이고 효율적이다. 또한 암의 유발, 진행 및 약물 대사 관련된 주요 유전자를 대상으로 높은 커버리지 (high coverage)로 시퀀싱함으로써 고해상도를 확보하여 기존 방법으로 검출이 되지 않았던 낮은 빈도 유전체 변이의 동정이 가능하다. 따라서, 본 발명의 방법을 이용하면 암 세포의 유전체 DNA의 변이와 약물 반응을 예측하는 유전자 변이를 포함하는 시료에서 다양한 유전자 변이를 동시에 높은 민감도와 정확도로 분석할 수 있고, 이러한 분석 결과를 바탕으로 환자 맞춤형 암 치료제를 효율적으로 탐색할 수 있다.
Description
본 발명은 암 세포의 유전체 DNA의 변이와 항암제의 약물 반응을 예측하는 유전자 변이를 검출하는데 사용하기 위한 조성물, 상기 조성물을 포함하는 차세대 염기 서열 분석(NGS) 기반 혼합 진단 패널, 및 상기 패널을 이용하여 상기 유전자 변이를 진단 및 검출하는 방법에 관한 것이다.
이하에 기술되는 내용은 단순히 본 발명과 관련되는 배경 정보만을 제공할 뿐 종래기술을 구성하는 것이 아니다.
암은 신체의 어느 조직에서나 발생할 수 있으며, 암세포는 일반적으로 인접한 조직에 침투하여 파괴하고, 점점 순환계를 침범하여 암 발생 부위로부터 멀리 떨어진 신체의 다른 부위로 전이되어 결국 숙주(예를 들면 사람)를 죽게 한다. 암세포는 비정상적으로 분열하며, 현미경 하에서 관찰해 보면 정상적인 조직이나, 세포의 형태를 잃고 비정상적인 기능을 나타낸다. 암은 종양의 형태에 따라 다양한 유전자 변이를 동반할 수 있으며, 암 발생과 진행에 있어 세포 돌연변이가 큰 영향을 미친다는 것이 보고되고 있다.
따라서 암세포로부터 유전자 변이를 검출하는 다양한 방법들이 연구되고 있으며, 검출된 돌연변이 정보는 암환자의 진단 및 정밀 맞춤 항암제 선정에 많은 도움을 줄 수 있다.
기존 유전체 변이 검출 방법들은 한 개의 유전체 변이만을 검출할 수 있도록 고안된 앰플리콘 (amplicon) 및 프로브 (probe)를 사용하고 있으므로, 암의 원인이 되는 다양한 체세포 돌연변이를 검출하기 위해서는 다른 유전체 변이의 검출을 위한 추가 실험이 필요하며, 기존에 밝혀진 변이 이외의 새로운 변이를 발견할 수 없다는 단점을 갖는다.
또한 기존의 방법은 각 유전체 변이 종류에 따라 별도의 검출 방법 (예, SNV: real-time PCR, CNV: CGH array; 또는 전좌: FISH;등)을 수행하기 때문에, 한명의 암조직을 대상으로 모든 종류의 변이를 검출하기 위해서는 많은 시간이 소요되고, 큰 비용이 발생하게 된다.
최근 차세대 염기 서열 분석(NGS) 기법의 도입으로 여러 개의 암 관련 유전자를 동시에 분석하는 것이 가능 해졌으나 진단 패널이 외국에서 주로 개발된 경우 한국인에는 제한적으로 적용된다. 뿐만 아니라 암 진단 및 표적 함암제를 위한 암유전체 뿐만 아니라 항암제를 대사하는 대사효소의 유전형 또한 약물 부작용 예방을 위해서 검사가 필요하고, 이를 위해 암 환자에게 발생한 다양한 약물대사 유전자를 포함하는 유전자 변이를 민감도 및 정확성이 높으면서 간편하고 신속하게 검출할 수 있는 방법이 필요하다.
예를 들어, 이리노테칸 (Irinotecan) 대사에 관여하는 UGT1A1의 경우에도 서양인에서는 UGT1A1*28이 항암제 부작용의 중요한 유전자 지표로 알려져 있으나 한국인과 일본인에서는 그 외에 UGT1A1*6 (한국인 18%)의 빈도가 높으므로 이를 고려해야하고, 타목시펜 (Tamoxifen) 대사에 관여하는 CYP2D6의 경우에도 한국인 특이적 빈도를 보이는 CYP2D6*52 (1%) 및 CYP2D6*60 (1%) 유전형도 고려해야한다.
따라서 한국인 호발 암 유전자 및 항암제 대사에 관련된 약물 반응을 예측하는 유전자의 변이를 검출하고 분석할 수 있는 한국인 맞춤형 암 진단 패널의 개발이 절실하다.
이에 본 발명자들은 한국인 맞춤형 암 진단 패널의 개발에 대해 연구하던 중 차세대 염기 서열 분석 (NGS) 기반 기술을 활용하면 관련 유전자의 전체 유전자를 대상으로 빠르고 효과적으로 한국인 호발 암 유전자 및 항암제 대사에 관련된 약물 반응을 예측 및 검출할 수 있음을 확인함으로써 본 발명을 완성하게 되었다.
따라서 본 발명의 목적은 암 세포의 유전체 DNA의 변이 및 약물 반응 유전자의 변이를 검출하기 위한 조성물을 제공하는 것이다.
또한, 본 발명의 다른 목적은 상기 조성물을 포함하는 차세대 염기 서열 분석(NGS) 기반 혼합 진단 패널을 제공하는 것이다.
또한, 본 발명의 목적은 상기 패널을 이용하여 상기 유전자 변이를 진단 및 검출하는 방법에 관한 것이다.
상기한 목적을 달성하기 위하여,
본 발명은 암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 프라이머를 이용하여 시료의 DNA로부터 표적 부위를 증폭하는 단계;
상기 증폭된 산물에 어댑터 (adapter)를 부착하는 단계;
상기 어댑터가 부탁된 산물을 증폭하여 최종 산물을 제조하는 단계; 및
상기 최종 산물의 서열을 분석하여 상기 표적 부위에 대한 앰플리콘 (amplicon)의 서열과 비교하여 유전자 변이를 검출하는 단계
를 포함하는 것을 특징으로 하는, 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(NGS) 방법을 제공한다.
본 발명에 있어서, 상기 암 유전자 또는 항암제 대사관련 유전자는 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 표적 부위는 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 프라이머는 표 2에 기재된 서열번호 1 내지 96의 프라이머로부터 선택되는 것일 수 있다.
본 발명에 있어서, 상기 앰플리콘은 표 3에 기재된 서열번호 97 내지 128의 서열로부터 선택되는 것일 수 있다.
본 발명에 있어서, 상기 증폭된 산물을 정제하는 단계를 추가로 포함할 수 있다.
또한 본 발명은 암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 프라이머를 포함하는 것을 특징으로 하는, 시료의 DNA로부터 표적 부위를 증폭하기 위한 조성물을 제공한다.
본 발명에 있어서, 상기 암 유전자 또는 항암제 대사관련 유전자는 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 표적 부위는 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 프라이머는 표 2에 기재된 서열번호 1 내지 96의 프라이머로부터 선택되는 것일 수 있다.
본 발명은 또한 상기 조성물, 어댑터 및 바코드 프라이머를 포함하는 것을 특징으로 하는, 암 유전자 및 항암제 대사 관련 유전자 변이 진단 패널을 제공한다.
본 발명의 암 세포의 유전체 DNA의 변이 및 약물 반응 유전자의 변이를 검출 및 진단하기 위한 방법은 주요 암 관련 유전자 및 약물 대사유전자의 다양한 유전자 변이를 단 한번의 실험을 통해 검출할 수 있어 각각의 유전체 변이마다 다른 플랫폼을 사용하는 종래 방법보다 매우 경제적이고 효율적이다. 또한 암의 유발, 진행 및 약물 대사 관련된 주요 유전자를 대상으로 높은 커버리지 (high coverage)로 시퀀싱함으로써 고해상도를 확보하여 기존 방법으로 검출이 되지 않았던 낮은 빈도 유전체 변이의 동정이 가능하다. 따라서 본 발명의 방법을 이용하면 암 세포의 유전체 DNA의 변이와 약물 반응을 예측하는 유전자 변이를 포함하는 시료에서 다양한 유전자 변이를 동시에 높은 민감도와 정확도로 분석할 수 있고, 이러한 분석 결과를 바탕으로 환자 맞춤형 암 치료제를 효율적으로 탐색할 수 있다.
도 1은 본 발명의 실시예 1에서 제작된 프라이머 및 앰플리콘을 이용하여 시료로부터 변이 유전자를 검출하는 방법을 간략하게 나타낸 도면이다.
도 2는 실시예 2에서 얻은 데이터를 상용화된 데이터 분석 소프트웨어인 Illumina VariantStudio를 활용해서 분석하는 과정을 나타내는 도면이다.
도 3은 실시예 2에서 얻은 데이터를 상용화된 데이터 분석 소프트웨어인 Illumina VariantStudio를 활용해서 분석한 결과를 나타내는 도면이다.
도 4는 실시예 2에서 얻은 데이터를 annotation & classification을 활용해서 분석한 결과를 나타내는 도면이다.
도 2는 실시예 2에서 얻은 데이터를 상용화된 데이터 분석 소프트웨어인 Illumina VariantStudio를 활용해서 분석하는 과정을 나타내는 도면이다.
도 3은 실시예 2에서 얻은 데이터를 상용화된 데이터 분석 소프트웨어인 Illumina VariantStudio를 활용해서 분석한 결과를 나타내는 도면이다.
도 4는 실시예 2에서 얻은 데이터를 annotation & classification을 활용해서 분석한 결과를 나타내는 도면이다.
본 발명은 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(NGS) 방법을 제공한다.
구체적으로, 상기 방법은 하기의 단계들을 포함할 수 있다:
암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 프라이머를 이용하여 시료의 DNA로부터 표적 부위를 증폭하는 단계;
상기 증폭된 산물에 어댑터 (adapter)를 부착하는 단계;
상기 어댑터가 부탁된 산물을 증폭하여 최종 산물을 제조하는 단계; 및
상기 최종 산물의 서열을 분석하여 상기 표적 부위에 대한 앰플리콘 (amplicon)의 서열과 비교하여 유전자 변이를 검출하는 단계.
본 발명에 있어서, 상기 암 유전자 또는 항암제 대사관련 유전자는, 바람직하게는 한국인에서 호발하는 암 유전자 및 상기 암을 치료하기 위한 항암제의 대사과정에 작용하는 유전자일 수 있으며, 더욱 바람직하게는 하기 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것일 수 있다.
| 유전자명 | 관련 암종 | 관련 약물 | 표적부위 |
| AKT1 | 유방암 | - | E17K |
| BRAF | 흑색종, 결장암, 폐암, 난소암 | Vemurafenib | V600E/K/R/M |
| EGFR | 폐암 | Elotinib,gefitinib | Exon18,19,20,21 |
| ERBB2 | 유방암, 폐암 | trastuzumab, neratinib, lapatinib | A775_G776YVMA |
| FOXL2 | 난소암 | - | C123W |
| KIT | 위암, 흑색종 | Imatinib,nilotinib,sorafenib | Exon8-11,13.14,17,18 |
| KRAS | 결장암, 위암, 폐암 | Cetuximab, panitumumab | Exon2, 3,4 |
| MET | 폐암, 결장암, 위암 | Trametinib | Focal Amplification |
| BRCA1 | 유방암 | Olabarib, veliparib, iniparib | Full CDS |
| BRCA2 | 유방암 | Full CDS | |
| NRAS | 결장암, 위암, 폐암 | binimetinib,palbociclib,sorafenib | Exon2, 3 |
| PDGFRA | 위암, 흑생종 | Sorafenib | Exon12,14,18 |
| PIK3CA | 페암, 유방암, 전립선암 | Idelisinib | Exon9,20 |
| RET | 폐암 | Vandetanib,Sorafenib | M918T |
| TP53 | 폐암, 흑색종, 난소암, 결장암 | - | Full CDS |
| UGT1A1 | 결장암 | Irinotecan | *6,*28 |
| CYP2D6 | 유방암 | Tamoxifen | Full CDS |
| TPMT | 혈액암 | Mercaptopurine, azatioprine | *2 *3B, *3C, *6 |
| MTHFR | 혈액암 | Thiopurin | 677C>T 1298A>C |
| DPYD | 위암 | 5'-Fluorouracil | Full CDS |
본 발명에 있어서, 상기 표적 부위는 상기 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 프라이머는 상기 유전자의 표적 부위를 증폭하기 위한 것으로, 하기 표 2에 기재된 프라이머로부터 선택되는 것일 수 있으며, 바람직하기로는 서열번호 1 내지 96의 프라이머로부터 선택되는 것일 수 있다.
| 정방향 프라이머명 | 서열번호 | 프라이머서열 | 역방향 프라이머명 | 서열번호 | 프라이머서열 |
| >520999_MTHFR_01_FOR | 1 | GGGCACAGGATGGGGAAGTCACAGC | >520999_MTHFR_01_REV | 2 | CGGGAGAACCAAACCGGAATGGTCAC |
| >520999_MTHFR_02_FOR | 3 | GAACCAGGGTCCCCACTCCAGCATC | >520999_MTHFR_02_REV | 4 | TGGGGAGCTGAAGGACTACTACCTCTTC |
| >520999_MTHFR_03_FOR | 5 | ACATCTTCAGCAGCTCCTCCTTGGG | >520999_MTHFR_03_REV | 6 | TACAGGAATGGCCTCCTGGGCATGTG |
| >520999_MTHFR_04_FOR | 7 | TCAGCGAACTCAGCACTCCACCCAGAG | >520999_MTHFR_04_REV | 8 | CTTCCGCTTTGTGAAGGCATGCACCGA |
| >520999_MTHFR_05_FOR | 9 | GAGGGAGCTTATGGGCTCTCCTGG | >520999_MTHFR_05_REV | 10 | CTGAAGCACTTGAAGGAGAAGGTGTCTGC |
| >520999_MTHFR_06_FOR | 11 | ATGTGTCAGCCTCAAAGAAAAGCTGC | >520999_MTHFR_06_REV | 12 | CTCTCCTGACTGTCATCCCTATTGGCAG |
| >520999_MTHFR_07_FOR | 13 | TTCAAGTGCTTCAGGTCAGCCTCAAAG | >520999_MTHFR_07_REV | 14 | TGGTCTCTTCATCCCTCGCCTTGAACA |
| >520999_DPYD_01_FOR | GCAGAAGAGCAATATTTGGCACCACTGG | >520999_DPYD_01_REV | GTGTATCAACTGTGGTAAATGCTACATGACCTG | ||
| >520999_DPYD_02_FOR | CATGTCTCATAGCATTCTAATTCCAGCAGG | >520999_DPYD_02_REV | AGCACTGCAGTACCTTGGAACATTTGGT | ||
| >520999_DPYD_03_FOR | TTCTTCATCAATCATAGCCACAACTTGCTC | >520999_DPYD_03_REV | GGAATGAGCTTGCTAAGTAATTCAGTGGC | ||
| >520999_DPYD_04_FOR | GGTACTGCAGTGCTTTTCCTATTACATCC | >520999_DPYD_04_REV | GCAACATTGTATAAAAACAGGAAAATGCTGAGTG | ||
| >520999_DPYD_05_FOR | GATCTGAAATAGAAACCAAGGCTGAGTTCTC | >520999_DPYD_05_REV | CAGAGTCCAGCTACTGTGAGTCACCAG | ||
| >520999_DPYD_06_FOR | TGTCCATGAGTTCAGCTATACGTGGAACTG | >520999_DPYD_06_REV | CTAGGTATGCAGTGCCATTCAGAATCAGG | ||
| >520999_DPYD_07_FOR | GCTTTGAGGCCAGTGCAGTAGTCTTCG | >520999_DPYD_07_REV | GTGAATTTGTTTGGAGAATTATCTCTTGGTTTGACAC | ||
| >520999_DPYD_08_FOR | GGGCACAAAACTCTTGCAACATGACCTTC | >520999_DPYD_08_REV | GTCTCAGGTCTGATGGGATTAAAATCTGATGGC | ||
| >520999_DPYD_09_FOR | GTTCGCTTTGCAATCCCCACTGCTG | >520999_DPYD_09_REV | GGGTTTTAACTATCGTGTCTTATAAGAGCTGC | ||
| >520999_DPYD_10_FOR | GGCATTCACCAACTTATGCCAATTCTCTTG | >520999_DPYD_10_REV | GTATGGCCCTGGACAAAGCTCCTTTC | ||
| >520999_DPYD_11_FOR | CCAATATGCAGCCGTTTTCTCACTGATG | >520999_DPYD_11_REV | GGGGCTTTTCTTTGTCAAAAGGAGACTC | ||
| >520999_DPYD_12_FOR | GGAGGGGACATTTCTATATGACTTCAATAATATTTTC | >520999_DPYD_12_REV | GGACATTAGTGTAGAAATGGCCGGATTGAAG | ||
| >520999_DPYD_13_FOR | GGAGTTGCGCTAGCAAGACCAAAAGG | >520999_DPYD_13_REV | GGAGGGGATGTAATATGAAACCAAGTATTGG | ||
| >520999_DPYD_14_FOR | GAAGGCAGTCATTCTTCTGGATATTGCTAGG | >520999_DPYD_14_REV | GCGGTAAAAGCCTTTCAGTGAATGAAATGAC | ||
| >520999_DPYD_15_FOR | CCTATTCCAATGAAAGCAGCTTTGTAGCC | >520999_DPYD_15_REV | GGGGAATCTCATAGAATTTTTGGCTGAC | ||
| >520999_DPYD_16_FOR | GCATGACATTTGCTGTTAATCTTTAGTGTAGAGC | >520999_DPYD_16_REV | TCTTTTCAGTACTTCTGAAATTCCTCAGTTCC | ||
| >520999_DPYD_17_FOR | GCACCAAGGTCCTTCATTAGCTCAATCTC | >520999_DPYD_17_REV | GGTCAAAGATTGGTCATTTTTCTACTGATGCC | ||
| >520999_DPYD_18_FOR | AAGATATTTGTGCATGGTGATGGTAGTGGG | >520999_DPYD_18_REV | CACTTGGTCTGACTTGTGGAATGGTATG | ||
| >520999_DPYD_19_FOR | GGTTGCATCCACCTACACAAAGATCAG | >520999_DPYD_19_REV | GGGAAGAAATAATGTTTGTCGTAATTTGGCTG | ||
| >520999_DPYD_20_FOR | GCTAAGACAAGCTGTATTCTGTACCCACAG | >520999_DPYD_20_REV | CCTAGATGCCTGAAATGTGCAGATGC | ||
| >520999_DPYD_21_FOR | GGCCTTGTTTGCAATACTTGTGATGAATG | >520999_DPYD_21_REV | GGACACGGACTCTGAATGAGTATAAGGTAG | ||
| >520999_DPYD_22_FOR | CTTGCCTTACAATGTGTGGAGTGAGG | >520999_DPYD_22_REV | TCCTGGCTTTAAATCCTCGAACACAAACT | ||
| >520999_DPYD_23_FOR | GTTTCTTGTCTAATTTCTTGGCCGAAGTGG | >520999_DPYD_23_REV | CTGGGAGTTTAAACAAATGCCAACATATTTCC | ||
| >520999_NRAS_01_FOR | CCAGAGTTAATCAACTGATGCAAACTCTTGC | >520999_NRAS_01_REV | GGTGCTAGTGGGAAACAAGTGTGATTTG | ||
| >520999_NRAS_02_FOR | CAGTTCGTGGGCTTGTTTTGTATCAACTG | >520999_NRAS_02_REV | GCCCAGCCTAATCTTGTTTTTCTTATGTTCTG | ||
| >520999_NRAS_03_FOR | CCCCATAAAGATTCAGAACACAAAGATCATCC | >520999_NRAS_03_REV | GATACAGCTGGACAAGAAGAGTACAGTGC | ||
| >520999_NRAS_04_FOR | CAAATACACAGAGGAAGCCTTCGCCTG | >520999_NRAS_04_REV | CCCCCAGGATTCTTACAGAAAACAAGTGG | ||
| >520999_NRAS_05_FOR | GCTGTATCCAGTATGTCCAACAAACAGGTTTCA | >520999_NRAS_05_REV | GGGATGCTTATTTAACCTTGGCAATAGCATTG | ||
| >520999_NRAS_06_FOR | AAGATGATCCGACAAGTGAGAGACAGG | >520999_NRAS_06_REV | GAAATGACTGAGTACAAACTGGTGGTGG | ||
| >520999_NRAS_07_FOR | GGATTGTCAGTGCGCTTTTCCCAACAC | >520999_NRAS_07_REV | GGGAAAGCTTTAAAGTACTGTAGATGTGGCTC | ||
| >520999_UGT1A1_01_FOR | GAAATTCCAGCCAGTTCAACTGTTGTTGCC | >520999_UGT1A1_01_REV | CCAAGTTAATGTTTGACTGTGTCACGTGAC | ||
| >520999_UGT1A1_02_FOR | GAACTCCCTGCTACCTTTGTGGACTGAC | >520999_UGT1A1_02_REV | TGACGACTTTGCTCCTGCCAGAGGTTC | ||
| >520999_UGT1A1_03_FOR | TGACACAGTCAAACATTAACTTGGTGTATCG | >520999_UGT1A1_03_REV | GTATCTTCCCAGCATGGGACACCACTG | ||
| >520999_UGT1A1_04_FOR | GAAGCAAAGACGCCATGGCTGTGGAGTC | >520999_UGT1A1_04_REV | CAACTATTTCATGTCCCCTCTGCTGCAGC | ||
| >520999_UGT1A1_05_FOR | GATGACAGACACTGGCTGAGCATGCTTGG | >520999_UGT1A1_05_REV | GGAATGGCACAGGGTACGTCTTCAAGG | ||
| >520999_UGT1A1_06_FOR | CACCTGACGCCTCGTTGTACATCAGAG | >520999_UGT1A1_06_REV | CTTGTATGTTTTGATCACACGCTGCAGG | ||
| >520999_UGT1A1_07_FOR | GGGAGTCTTTTGTTAGTCTCGGGCATAATG | >520999_UGT1A1_07_REV | CATGACATCAAAGCTGCTTTCTGCCAG | ||
| >520999_UGT1A1_08_FOR | CTGTTCCCACTTACTGCACAACAAGGAGC | >520999_UGT1A1_08_REV | GCACTGGGTAGCCTCAAATTCCAGGC | ||
| >520999_UGT1A1_09_FOR | CTCTGCCCACTGTATTCTTCTTGCATGC | >520999_UGT1A1_09_REV | CAGAAAGTTCTGTGAAAAGGCAATGAGCATG | ||
| >520999_UGT1A1_10_FOR | CTCATTCAGATCACATGACCTTCCTGCAGC | >520999_UGT1A1_10_REV | CAGCCAGACAGATGCAGAGCTCAATAGG | ||
| >520999_UGT1A1_11_FOR | CGTATGCAACCCTTGCCTCAGAATTCC | >520999_UGT1A1_11_REV | GGATTTTGGTGAAGGCAGTTGATTCCACC | ||
| >520999_UGT1A1_12_FOR | GTGAAGGATTACCCTAGGCCCATCATGC | >520999_UGT1A1_12_REV | CTCAGCATATATCTGGGGCTAGTTAATCGATCC | ||
| >520999_FOXL2_01_FOR | CTAGCTGCTGAACTAAGGAGCGACAG | >520999_FOXL2_01_REV | CCATAGACCTGGTTGTACTGTAGCTTGTTG | ||
| >520999_FOXL2_02_FOR | GGCACAACAAAAGTGCTCTAGTTCTCTCTAG | >520999_FOXL2_02_REV | CAAACCTCCTGCTTCTCCCACCTTGG | ||
| >520999_FOXL2_03_FOR | CAGAGACACAAGACCGCCTAGGTCGG | >520999_FOXL2_03_REV | TGCAAGTCCTGGAGGCAGGGAGATC | ||
| >520999_FOXL2_04_FOR | CAAAAAGCAGGGAGGACGAGCCGTCC | >520999_FOXL2_04_REV | GGGATGGTTTCGTCTACCAATTGGGGAAG | ||
| >520999_FOXL2_05_FOR | CCAGGACTTGCATCGAAAGATCCGG | >520999_FOXL2_05_REV | GGGCTCTTCTGGAAGATTTGCGTTAGAG | ||
| >520999_FOXL2_06_FOR | GCCATCTTTTATTGGATATATTCTCCTAACTCTGAAC | >520999_FOXL2_06_REV | GGAAGTATTGTGGCCTTGGAGTTTGC | ||
| >520999_FOXL2_07_FOR | GGGCTAAAGCTTTTGATTTTCATATTTGG | >520999_FOXL2_07_REV | GGGTTTTGTTTGAAAGATCGGTGGAAC | ||
| >520999_FOXL2_08_FOR | CAAGGCCACAATACTTCCCCGGTTGC | >520999_FOXL2_08_REV | GTTTCCTCCGCTATCAGTCCCGTCGC | ||
| >520999_FOXL2_09_FOR | CGATCTTTCAAACAAAACATTAGCCAGCC | >520999_FOXL2_09_REV | CGAACCCTCTTGAAGCTGTTCAGAAGC | ||
| >520999_FOXL2_10_FOR | CAGAATACAGGCCGTGGAGCCAGGCTG | >520999_FOXL2_10_REV | GCCCGAGAAAGGGACGGACCAATAC | ||
| >520999_FOXL2_11_FOR | GAACAGCTTCAAGAGGGTTCGCGGAG | >520999_FOXL2_11_REV | CAGACCTCTTGGCCTTCTCTCACAGGTC | ||
| >520999_FOXL2_12_FOR | CTAGCCATGGACTGCACGGCAGTC | >520999_FOXL2_12_REV | CACAAGCGTCTCCTGCCCAAATTCACC | ||
| >520999_FOXL2_13_FOR | GAGAGAAGGCCAAGAGGTCTGCGCTG | >520999_FOXL2_13_REV | CCTGTGCTCACCTCCCTGACCTCTG | ||
| >520999_FOXL2_14_FOR | GAAAGAGACGAGCCCAGTAGAAAGCGC | >520999_FOXL2_14_REV | GGGTCCCTCTGTGCTTTTTCGCTTTCC | ||
| >520999_FOXL2_15_FOR | GACATAAACTGAGGGGACAAAGAGGAGC | >520999_FOXL2_15_REV | GTGCAGACGGATGCGAGGATGCAG | ||
| >520999_FOXL2_16_FOR | GAGACAAGAGGGTGTGAGGTCAGGCTG | >520999_FOXL2_16_REV | CTCTTACTGGGACCACGACAGCAAGAC | ||
| >520999_FOXL2_17_FOR | GCATGCGGTGGGCTCTCAGAGATCGAG | >520999_FOXL2_17_REV | CAACTCAGACCTGCCAGCCCAGCCAC | ||
| >520999_FOXL2_18_FOR | CAATGCATCATGGCGAGCTCGGGCTG | >520999_FOXL2_18_REV | CATCAGCACGCACACCATCTGCACGC | ||
| >520999_FOXL2_19_FOR | GAGGACAAGTGGTGCGGTGGGGCAG | >520999_FOXL2_19_REV | GTACAGACCGTACACACGCGTGCAGAGCATG | ||
| >520999_FOXL2_20_FOR | GCCTCCCAGGCCATTGTACGAGTTCACTACG | >520999_FOXL2_20_REV | CAGAGACTGAAGCAGCTGCGGCTGCAG | ||
| >520999_FOXL2_21_FOR | CAGACACGCCAGCCCCTTGACCACAG | >520999_FOXL2_21_REV | CCCAAGTACCTGCAGTCTGGCTTCCTC | ||
| >520999_FOXL2_22_FOR | CATCTGGCAGGAGGCATAGGGCATG | >520999_FOXL2_22_REV | CACTTCCAGCACGGCAAGGGGCTCT | ||
| >520999_FOXL2_23_FOR | GTTGAGGAAGCCAGACTGCAGGTACTTG | >520999_FOXL2_23_REV | CGAAGACATGTTCGAGAAGGGCAACTACCG | ||
| >520999_FOXL2_24_FOR | GAGACAGACGGAAGGGCCTCTTCATGC | >520999_FOXL2_24_REV | GGCAAAATAGCATCCGCCACAACCTCAGC | ||
| >520999_FOXL2_25_FOR | CAGAGTCCAGTAGTTGCCCTTGCGCTC | >520999_FOXL2_25_REV | GAGAGAGGAGAAGAGGCTCACGCTGTC | ||
| >520999_FOXL2_26_FOR | GAACGGGAACTTCGCGATGATGTACTGG | >520999_FOXL2_26_REV | GACAGAACAGACCCGGAGAAGCCGGAC | ||
| >520999_FOXL2_27_FOR | GCGATGAGCGCCACGTACGAGTACG | >520999_FOXL2_27_REV | CTTTGTCATGATGGCCAGCTACCCCGAGC | ||
| >520999_FOXL2_28_FOR | CTCCTTGACTGTGCGACCGGTCTCTG | >520999_FOXL2_28_REV | GACTCGTGCGCCCCAACTCTTTGCC | ||
| >520999_FOXL2_29_FOR | GGGTAGCTGGCCATCATGACAAAGCC | >520999_FOXL2_29_REV | CAGCGACAGGCCTCCAGCAAGCAAG | ||
| >520999_FOXL2_30_FOR | GGGTCTCAAACTTCTGGAGACTGCGGATG | >520999_FOXL2_30_REV | GACAAGGCCGGAGCCCCTCTCTGTTG | ||
| >520999_FOXL2_31_FOR | GAGTGGAGCTCAGCCTCTGGCCATG | >520999_FOXL2_31_REV | GAGACACGGGTCGTGCTTCGGGAAAC | ||
| >520999_FOXL2_32_FOR | CTTGGTGGGTTTTCTTTTCTGCGCTCTTCC | >520999_FOXL2_32_REV | CATGACGCAAGCGGAACTCGGTGGAG | ||
| >520999_PIK3CA_01_FOR | GGGTGGCAGTCAAACCTTCTCTCTTATGTA | >520999_PIK3CA_01_REV | GCTAAATCCTGCTTCTCGGGATACAGACC | ||
| >520999_PIK3CA_02_FOR | GCAGTGTGGTAAAGTTCCCAGATATGTCAG | >520999_PIK3CA_02_REV | GGGCCTTTTCCATAGAGAAAGTATCTACCTAAATCC | ||
| >520999_PIK3CA_03_FOR | GCATGGTGAAAGACGATGGACAAGTAATGG | >520999_PIK3CA_03_REV | GCGGTGATCCAAAAAGTGTCCAAAATCTATATG | ||
| >520999_PIK3CA_04_FOR | CGGGCAAGACATTTTGTATCTGCATATATCAAAC | >520999_PIK3CA_04_REV | GGTGGGCTCCTTTACTAATCACTATTAAGAAATCC | ||
| >520999_PIK3CA_05_FOR | GGGTTATAAACGAGAACGTGTGCCATTTG | >520999_PIK3CA_05_REV | GGGGATTGTTTCTAATAGAGCAGCCAGAAC | ||
| >520999_PDGFRA_01_FOR | CTTCAGTGAAGCTCTGGTGCACTGGGA | >520999_PDGFRA_01_REV | GAGTCATAAGGCAGCTGCATCGGGTCC | ||
| >520999_PDGFRA_02_FOR | GCTGGAGGGTCATTGAATCAATCAGCC | >520999_PDGFRA_02_REV | GGCCTATAAATTGTAAAGTTGTGTGCAAGGG | ||
| >520999_PDGFRA_03_FOR | TGAGAACAGGAAGTTGGTAGCTCAGCT | >520999_PDGFRA_03_REV | GTGGCCCCAGGTGAGTCATTATCTTCA | ||
| >520999_PDGFRA_04_FOR | GGCCAGATCCAGTGAAAAACAAGCTC | >520999_PDGFRA_04_REV | AAGCCTGATTGAACAGTTTTCACAACC | ||
| >520999_PDGFRA_05_FOR | CAGCTACAGATGGCTTGATCCTGAGTC | >520999_PDGFRA_05_REV | CACTGCCTTTCGACACATAGTTCGAATC | ||
| >520999_PDGFRA_06_FOR | GTGAAGATCTGTGACTTTGGCCTGGC | >520999_PDGFRA_06_REV | GCACCGAATCTCTAGAAGCAACACCTGAC | ||
| >520999_KIT_01_FOR | GAATGTTGCTGAGGTTTTCCAGCACTC | >520999_KIT_01_REV | CATCTCACCTCTGCTCAGTTCCTGGAC | ||
| >520999_KIT_02_FOR | GCAGGATTCCCAGAGCCCACAATAG | >520999_KIT_02_REV | GGGAAATTCAAGTGAATTGCAGTCCTTCCC | ||
| >520999_KIT_03_FOR | GGGTATGCCACATCCCAAGTGTTTTATG | >520999_KIT_03_REV | GCACTAGAATCTATAGAACTCTGAACCACTAGC | ||
| >520999_KIT_04_FOR | CAGTGGATGTGCAGACACTAAACTCATCTG | >520999_KIT_04_REV | GCAGAAGTCTTGCCCACATCGTTGTAAG | ||
| >520999_KIT_05_FOR | GTGCATTCAAGCACAATGGCACGGTTG | >520999_KIT_05_REV | GGATCATGACTGATATGGTAGACAGAGCC | ||
| >520999_KIT_06_FOR | GTGGTAGAGATCCCATCCTGCCAAAGT | >520999_KIT_06_REV | CACATCATGCCAGCTACGATTACGAAACC | ||
| >520999_KIT_07_FOR | CAAATCCATCCCCACACCCTGTTCAC | >520999_KIT_07_REV | GTCTCAGTCATTAGAGCACTCTGGAGAG | ||
| >520999_KIT_08_FOR | GTTCTCTCTCCAGAGTGCTCTAATGACTG | >520999_KIT_08_REV | GTGATCATAAGGAAGTTGTGTTGGGTCTATG | ||
| >520999_KIT_09_FOR | TATGAAGTACAGTGGAAGGTTGTTGAGGAG | >520999_KIT_09_REV | GAGTTCCTTAAAGTCACTGTTATGTGTACCC | ||
| >520999_KIT_10_FOR | TGCACAAATGGTCCTTCAATTCCACC | >520999_KIT_10_REV | CTTGAGCATCTTTACAGCGACAGTCATGG | ||
| >520999_KIT_11_FOR | GGAAGGTTGTTGAGGCAACTGCTTATGG | >520999_KIT_11_REV | CATGCATTTTAGCAAAAAGCACAACTGGC | ||
| >520999_KIT_12_FOR | GTACTGCATGCGCTTGACATCAGTTTG | >520999_KIT_12_REV | CAATGGTGCAGGCTCCAAGTAGATTCAC | ||
| >520999_KIT_13_FOR | GTCTGAACTCAAAGTCCTGAGTTACCTTGG | >520999_KIT_13_REV | GGCTTCCAAGCAGTTTATAATCTAGCATTGCC | ||
| >520999_KIT_14_FOR | GCTGAATATTAATGGCCATGACCACCCTTG | >520999_KIT_14_REV | GGATCACCATAGCAACAATATTCTGTAATGACC | ||
| >520999_KIT_15_FOR | GGCTCACCTTCTTTCTAACCTTTTCTTATGTGC | >520999_KIT_15_REV | GCAGCTTCTGCATGATCTTCCTGCTTTG | ||
| >520999_KIT_16_FOR | GGGCTTTTGAATTTTTTGAGAAGAAAACGTGATTC | >520999_KIT_16_REV | CCTTATGACCCCATGAACTGCCTGTC | ||
| >520999_KIT_17_FOR | GGGGAATTTAAATGGTTTTCTTTTCTCCTCCAACC | >520999_KIT_17_REV | GGAATCATTCTTGATGTCTCTGGCTAGACC | ||
| >520999_KIT_18_FOR | GCTCCTTACTCATGGTCGGATCACAAAG | >520999_KIT_18_REV | GGCACAGGAAACAATTTTTATCGAAAGTTGAAAC | ||
| >520999_KIT_19_FOR | CTCCACATTTCAGCAACAGCAGCATC | >520999_KIT_19_REV | CCCATAGGACCAGACGTCACTTTCAAACG | ||
| >520999_KIT_20_FOR | CTGTGAAGTGGATGGCACCTGAAAG | >520999_KIT_20_REV | CCAATGAAACTTCAAGAAGATGCTCTGAGTC | ||
| >520999_TPMT_01_FOR | 15 | GGGCAATTCCTCAAAAACATGTCAGTGTG | >520999_TPMT_01_REV | 16 | GCAATATACGTTGTCTTGAGAAGGTTGATGC |
| >520999_TPMT_02_FOR | 17 | GCTTTTCAAAAAGACAGTCAATTCCCCAAC | >520999_TPMT_02_REV | 18 | GGGAGAATCCCTGATGTCATTCTTCATAG |
| >520999_TPMT_03_FOR | 19 | GGGATCAAGAAACTAGGCAACTGGTAAAAG | >520999_TPMT_03_REV | 20 | CCTTCTTGTGTTCTTTGTAGCTATGCAGATAC |
| >520999_TPMT_04_FOR | 21 | GTTGGATCATAAGAAAGAACACACAGGAGATAC | >520999_TPMT_04_REV | 22 | CAGCGAAAGTAACTTCTGGCTTCCTTCC |
| >520999_TPMT_05_FOR | 23 | GGGAAGTCTAAGCTGATTTTCTAGAACCCAG | >520999_TPMT_05_REV | 24 | GGGCAAATTTGACATGATTTGGGATAGAGG |
| >520999_TPMT_06_FOR | 25 | GGGTTACTTACCATTTGCGATCACCTGG | >520999_TPMT_06_REV | 26 | GATTGTTGAAGTACCAGCATGCACCATG |
| >520999_TPMT_07_FOR | 27 | GGCTGAGAAAAACTTTTGTGGGGATATGGATAC | >520999_TPMT_07_REV | 28 | GTGTAGTTGGTGTGGAAATCAGTGAACTTGG |
| >520999_TPMT_08_FOR | 29 | GGGTTCTTCTGAGTAAGAAAGATTCTGCTCTG | >520999_TPMT_08_REV | 30 | GAGGGTGCTTACTCTAATATAACCCTCTATTTAGTC |
| >520999_EGFR_01_FOR | TTTCCAGCATGGTGAGGGCTGAGGTGA | >520999_EGFR_01_REV | GCACCGGAGCCCAGCACTTTGATC | ||
| >520999_EGFR_02_FOR | CCAAGCTCTCTTGAGGATCTTGAAGGAAAC | >520999_EGFR_02_REV | GGAAATATACAGCTTGCAAGGACTCTGGG | ||
| >520999_EGFR_03_FOR | GCATGTGGCACCATCTCACAATTGCCA | >520999_EGFR_03_REV | CCACACAGCAAAGCAGAAACTCACATC | ||
| >520999_EGFR_04_FOR | GAGAAAGTTAAAATTCCCGTCGCTATCAAGG | >520999_EGFR_04_REV | GAGAAAAGGTGGGCCTGAGGTTCAGAG | ||
| >520999_EGFR_05_FOR | CCTCCTTCTGGCCACCATGCGAAGC | >520999_EGFR_05_REV | CGAAGGGCATGAGCTGCGTGATGAG | ||
| >520999_EGFR_06_FOR | GACTGCTGGGCATCTGCCTCACCTC | >520999_EGFR_06_REV | CCGTATCTCCCTTCCCTGATTACCTTTGC | ||
| >520999_EGFR_07_FOR | CTGGACTATGTCCGGGAACACAAAGAC | >520999_EGFR_07_REV | CATGGCAAACTCTTGCTATCCCAGGAG | ||
| >520999_EGFR_08_FOR | GATGCAGAGCTTCTTCCCATGATGATCTG | >520999_EGFR_08_REV | CAGTTTGGCCAGCCCAAAATCTGTGATC | ||
| >520999_EGFR_09_FOR | GCAGCCAGGAACGTACTGGTGAAAAC | >520999_EGFR_09_REV | CAGCTAGTGGGAAGGCAGCCTGGTC | ||
| >520999_MET_01_FOR | CACAATCATACTGCTGACATACAGTCGGAG | >520999_MET_01_REV | CGGGAAATAAGAAGAATTTATGGTATTGCCTACAAAG | ||
| >520999_MET_02_FOR | GAGCCAAAGTCCTTTCATCTGTAAAGGACC | >520999_MET_02_REV | GGTAAAACATCAATGTAGGACTGGTCCGTC | ||
| >520999_MET_03_FOR | CAGCTTGCTAGACAAATAGGAGCCAGC | >520999_MET_03_REV | TCCGTAAAAATGCTGGAGACATCTCACATTG | ||
| >520999_MET_04_FOR | CAATGGATCGATCTGCCATGTGTGCATTC | >520999_MET_04_REV | CCTCACAGTTTATAAGTGGGGAACTGATGTG | ||
| >520999_MET_05_FOR | GCATTTTTACGGACCCAATCATGAGCACTG | >520999_MET_05_REV | GGGCAACTATTTTGGATAAACACCATTTACAAAG | ||
| >520999_MET_06_FOR | GGGTAAGTCACATCAGTTCCCCACTTATAAACTG | >520999_MET_06_REV | CGGGTAGGTGTAAATGAAGATTCAATTCCTC | ||
| >520999_MET_07_FOR | GCCAGGTGTTTATCCAAAATAGTTGCAG | >520999_MET_07_REV | GGCTGAAATGTCACGAACATGCAAATCTG | ||
| >520999_MET_08_FOR | ACTGTGCCTCTGACCTGTAATCAGTGC | >520999_MET_08_REV | CTAACTGAATTCAGGTTTTTCCCAACACCTG | ||
| >520999_MET_09_FOR | CTATTTTGCTTTGCCAGTGGTGGGAGC | >520999_MET_09_REV | GGTTAGAGGCAAAGATGCAGAGCTCTG | ||
| >520999_MET_10_FOR | CGGGAACACTGGAGCATAATTGAGGAATCTC | >520999_MET_10_REV | GGCGAAAAGTCAGCATTTAGCCATGATAG | ||
| >520999_MET_11_FOR | GGGACCCAAAGTGCTACAACCTGTGTAG | >520999_MET_11_REV | CTGGTTGAACTATTACTTTTCCAAGGACGG | ||
| >520999_MET_12_FOR | CCATGAGTTCTGGGCACTGGGTCAAAG | >520999_MET_12_REV | CTTACACTTCGGGCACTTACAAGCCTATC | ||
| >520999_MET_13_FOR | GCAGTGAATTAGTTCGCTACGATGCAAGAG | >520999_MET_13_REV | CCTTCTGGAAAAGTAGCTCGGTAGTCTACAG | ||
| >520999_MET_14_FOR | GTAAGTGCCCGAAGTGTAAGCCCAACTAC | >520999_MET_14_REV | GCAATGTCACAACCCACTGAGGTATATGTATAGG | ||
| >520999_MET_15_FOR | GGGTGGTTTACCATTTCATTGCTCTTCCTATC | >520999_MET_15_REV | GGCTTTCATGATGATTCCCTCGGTCAG | ||
| >520999_MET_16_FOR | CCACCACTGGATTTCTCAGGAATCACTGAC | >520999_MET_16_REV | CTCCATGTTTCATGTATGGTAGGACCACC | ||
| >520999_MET_17_FOR | CCTGGGAATCTGCCTGCGAAGTGAAG | >520999_MET_17_REV | CCTATTTTGAAGGGATGGCTGGCTTACAGC | ||
| >520999_BRAF_01_FOR | CTTTCTAGTAACTCAGCAGCATCTCAGGG | >520999_BRAF_01_REV | GGTGATTTTGGTCTAGCTACAGTGAAATCTCG | ||
| >520999_BRAF_02_FOR | GGATCCAGACAACTGTTCAAACTGATGG | >520999_BRAF_02_REV | GCCTAAACTCTTCATAATGCTTGCTCTGATAGG | ||
| >520999_RET_01_FOR | CCTGGCCTTCTCCTTTACCCCTCCTTC | >520999_RET_01_REV | CCCACACTTACACATCACTTTGCGTGG | ||
| >520999_RET_02_FOR | GATTCCAGTTAAATGGATGGCAATTGAATCCC | >520999_RET_02_REV | CGTGGCCCCACTACATGTATAAGGG | ||
| >520999_BRCA2_24_FOR | CTGAAGTGGAACCAAATGATACTGATCC | >520999_BRCA2_24_REV | AAGGGTTAGTTGAGACCATTCACAG | ||
| >520999_BRCA2_25_FOR | GTAGCAAATCAGAAGCCCTTTGAGAG | >520999_BRCA2_25_REV | GGTCACATGAAGAAATATGCAATAGGGG | ||
| >520999_BRCA2_26_FOR | GGTCTCAACTAACCCTTTCAGGTC | >520999_BRCA2_26_REV | CTTTCTTTCTTTTGTTCTCTGTGTCTAATAGGTC | ||
| >520999_BRCA2_27_FOR | CCCTATTGCATATTTCTTCATGTGACC | >520999_BRCA2_27_REV | CCACTGTTTCCTCATTTAATGGCTTCT | ||
| >520999_BRCA2_28_FOR | GAGAATTCTTTGCCACGTATTTCTAGCC | >520999_BRCA2_28_REV | TGGAGAAGTTCCAGATATTGCCTGC | ||
| >520999_BRCA2_29_FOR | GAGAGATGAAGAGCAGCATCTTGAAT | >520999_BRCA2_29_REV | CTTGCATTGAAAGTCTCTTTAGGTGATTCTC | ||
| >520999_BRCA2_30_FOR | TGGAACTTCTCCAGTGGCTTCTTC | >520999_BRCA2_30_REV | GTCCACTTTCAGAGGCTTCAGTTTC | ||
| >520999_BRCA2_31_FOR | GAGACTTTCAATGCAAGTTTTTCAGGTC | >520999_BRCA2_31_REV | GGCTGGCCAGCTTCCATTATCAATTAA | ||
| >520999_BRCA2_32_FOR | CATACTGTTTGCTCACAGAAGGAGG | >520999_BRCA2_32_REV | AACTTATTTGTTTTCTTTTTCAAAGTGGATATTAAAC | ||
| >520999_BRCA2_33_FOR | AAGCTGGCCAGCCACCACCACAC | >520999_BRCA2_33_REV | CAGTTAATTAGTTCTGATTTTTGGTCTTTCGG | ||
| >520999_BRCA2_34_FOR | GCTTTGAAGAATGCAGGTTTAATATCCAC | >520999_BRCA2_34_REV | GCATTTGCTTCAAACTGGGCTGAACAG | ||
| >520999_BRCA2_35_FOR | GAAACATCTTATAAAGGAAAAAAAATACCGAAAGACC | >520999_BRCA2_35_REV | CACAGAAGGAATCGTCATCTATAAAACTATATGTAC | ||
| >520999_BRCA2_36_FOR | GTGATTGATGGTACTTTAATTTTGTCACTTTGTG | >520999_BRCA2_36_REV | CTAGAACATTTCCTCAGAATTGTCCC | ||
| >520999_BRCA2_37_FOR | GAATGATTCTGAAGAACCAACTTTGTCC | >520999_BRCA2_37_REV | GCTTCTTTATAATCAAGATCCTGAGAG | ||
| >520999_BRCA2_38_FOR | CTGAGGAAATGTTCTAGAAATGAAACATGTTC | >520999_BRCA2_38_REV | GCTTTTTGGATCATTTTCACACTGTCC | ||
| >520999_BRCA2_39_FOR | CCCAGAAGCTGATTCTCTGTCATG | >520999_BRCA2_39_REV | GGATTGAAAGTCAGTATCACTGTATTCC | ||
| >520999_BRCA2_40_FOR | GCAGCATGTCACCCAGTACAAC | >520999_BRCA2_40_REV | GACTAGGTTTGACAGAACATCCTTGG | ||
| >520999_BRCA2_41_FOR | GTCTTTTATATGATCATGAAAATGCCAGCACTC | >520999_BRCA2_41_REV | CATAATTGTTACCTTTGAGCTTGTCTGAC | ||
| >520999_BRCA2_42_FOR | CCTAGTCATGATTTCTAGAGGCAAAG | >520999_BRCA2_42_REV | GCACATACATCTTGATTCTTTTCCATGGG | ||
| >520999_BRCA2_43_FOR | CAAAATGTCAGACAAGCTCAAAGGTAAC | >520999_BRCA2_43_REV | TTCAGGTGGCAACAGCTCAACG | ||
| >520999_BRCA2_44_FOR | CCCATGGAAAAGAATCAAGATGTATGTGC | >520999_BRCA2_44_REV | CTCTTAGATTTGTGTTTTGGTTGAATTGTACC | ||
| >520999_BRCA2_45_FOR | CCTGAAAAATACATGAGAGTAGCATCACC | >520999_BRCA2_45_REV | GAGAAAAGTTCTTCAGAGTCTGGATTGACAG | ||
| >520999_BRCA2_46_FOR | GTACAATTCAACCAAAACACAAATCTAAGAGTAATCC | >520999_BRCA2_46_REV | GCAAGATTATTCCTTTCATTAGCTACTTGG | ||
| >520999_BRCA2_47_FOR | CTCTGAAGAACTTTTCTCAGACAATGAG | >520999_BRCA2_47_REV | CATGGTAGAGTTCTTGAAAATGGGTTCG | ||
| >520999_BRCA2_48_FOR | CTAAGGAACTTCATGAAACAGACTTGAC | >520999_BRCA2_48_REV | GTTCTCCTCTGCAAGAACATAAACCAAATC | ||
| >520999_BRCA2_49_FOR | TATGGAGACACAGGTGATAAACAAGCAACC | >520999_BRCA2_49_REV | CTGGTATTTTATCTATATTCAAGGAGATGTCCG | ||
| >520999_BRCA2_50_FOR | GCAGCATATAAAAATGACTCTAGGTCAAG | >520999_BRCA2_50_REV | ATTTCCTTATTTGAAGCTGTTCTGAAGC | ||
| >520999_BRCA2_51_FOR | CAAATGGGCAGGACTCTTAGGTCC | >520999_BRCA2_51_REV | CAACACAAGCTAAACTAGTAGGATATTGTTCTTC | ||
| >520999_BRCA2_52_FOR | CAAGCTCTCTGAACATAACATTAAGAAGAGCA | >520999_BRCA2_52_REV | CAACTACACTACTCTGTAAATGTGCAGATACAG | ||
| >520999_BRCA2_53_FOR | CAAAAGAAACTGAGCAAGCCTCAG | >520999_BRCA2_53_REV | CTGTAATTTCTGCCTTTTGGCTAGGTG | ||
| >520999_KRAS_01_FOR | 31 | GGGGACATAACAGTTATGATTTTGCAGAAAACAG | >520999_KRAS_01_REV | 32 | GCCTTCTAGAACAGTAGACACAAAACAGGC |
| >520999_KRAS_02_FOR | 33 | GGCTTACCTGTCTTGTCTTTGCTGATGTTTC | >520999_KRAS_02_REV | 34 | GTTAAGGACTCTGAAGATGTACCTATGGTCC |
| >520999_KRAS_03_FOR | 35 | GTTTTGTGTCTACTGTTCTAGAAGGCAAATCAC | >520999_KRAS_03_REV | 36 | GGGACAAAAGTTGTGGACAGGTTTTGAAAG |
| >520999_KRAS_04_FOR | 37 | CCAGAGGACTATAATTACTCCTTAATGTCAGC | >520999_KRAS_04_REV | 38 | GAGTACAGTGCAATGAGGGACCAGTAC |
| >520999_KRAS_05_FOR | 39 | GGCAAATACACAAAGAAAGCCCTCCC | >520999_KRAS_05_REV | 40 | GGGTGCACTGTAATAATCCAGACTGTG |
| >520999_KRAS_06_FOR | 41 | CCTTTATCTGTATCAAAGAATGGTCCTGCAC | >520999_KRAS_06_REV | 42 | GACTGAATATAAACTTGTGGTAGTTGGAGCTGG |
| >520999_KRAS_07_FOR | 43 | CTGAATTAGCTGTATCGTCAAGGCACTC | >520999_KRAS_07_REV | 44 | GGGAAGGTACTGGTGGAGTATTTGATAGTG |
| >520999_BRCA2_01_FOR | ATTTTCCAGCGCTTCTGAGTTTTACC | >520999_BRCA2_01_REV | GCCTCTCTTTGGATCCAATAGGCA | ||
| >520999_BRCA2_02_FOR | ACTTATTTACCAAGCATTGGAGGAATATCG | >520999_BRCA2_02_REV | GCATTTTTTAGAAAACACTTTCTCGGTGT | ||
| >520999_BRCA2_03_FOR | TTTTTTCCTTATGATCTTTAACTGTTCTGGGTC | >520999_BRCA2_03_REV | CAGGTTCAGAATTATAGGGTGGAGC | ||
| >520999_BRCA2_04_FOR | GGACCAATAAGTCTTAATTGGTTTGAAGAAC | >520999_BRCA2_04_REV | GTTGAAGCCAGCTGATTATAAGATGGT | ||
| >520999_BRCA2_05_FOR | AACAACAATTACGAACCAAACCTATTTAAAACTC | >520999_BRCA2_05_REV | TGCATTACTTACCTAAGTCTAATTTGAATTTATCT | ||
| >520999_BRCA2_06_FOR | AAAGAGCAAGGGCTGACTCTGC | >520999_BRCA2_06_REV | GAGCTAAGATTTTAACACAGGTTTGCC | ||
| >520999_BRCA2_07_FOR | AGAATGCAAATTTATAATCCAGAGTATATACATTCTCACTGA | >520999_BRCA2_07_REV | TAATAGCTTCATCATACCTTTCACTAAGAC | ||
| >520999_BRCA2_08_FOR | GATCAAGCAGATGATGTTTCCTGTCCAC | >520999_BRCA2_08_REV | TGTAAGATCTTCTACCAGGCTCTTAGCC | ||
| >520999_BRCA2_09_FOR | GCCAGTTTTTTAAAATAACCTAAGGGATTTGCTTTG | >520999_BRCA2_09_REV | ACATTTCTAGTATTCTAAGAATAAAAAGCATTG | ||
| >520999_BRCA2_10_FOR | CACATGTAACACCACAAAGAGATAAGTCAGGT | >520999_BRCA2_10_REV | ACCAGGTAGAATATTTACCTTCACAAACTTTGGTGT | ||
| >520999_BRCA2_11_FOR | CCCCAGTGGTATGTGGGAGTT | >520999_BRCA2_11_REV | GCTAGGTACAGATTTGTAAATCTCAGGG | ||
| >520999_BRCA2_12_FOR | TCAGTAAACGTTAAGTGAAATAAAGAGTGAATG | >520999_BRCA2_12_REV | AAGACATATCAGGATCCACCTCAGCTCCTAGAC | ||
| >520999_BRCA2_13_FOR | CTATTTTCTTTCCTCCCAGGGTCG | >520999_BRCA2_13_REV | CCTATGAGCACAGTAGAACTAAGGG | ||
| >520999_BRCA2_14_FOR | GAGCTGAGGTGGATCCTGATATGT | >520999_BRCA2_14_REV | GAGATGACAATTATCAACCTCATCTGCTC | ||
| >520999_BRCA2_15_FOR | TCAAATGTGTCATGTAATCAAATAGTAGATGTGC | >520999_BRCA2_15_REV | GTCATATTTACTTACAGCAGTAGTATCATGAGG | ||
| >520999_BRCA2_16_FOR | TCTTACAGTCAGAAATGAAGAAGCATCTG | >520999_BRCA2_16_REV | GAGACTTTCTCAAAGGCTTAGATAAATTACAGATTAGG | ||
| >520999_BRCA2_17_FOR | GGGACTACTACTATATGTGCATTGAGAGT | >520999_BRCA2_17_REV | GTGTTTTCACTGTCTGTCACAGAAGCGA | ||
| >520999_BRCA2_18_FOR | GCTATTTTTCCAATCATGATGAAAGTCTGAAG | >520999_BRCA2_18_REV | AACCTGTAGTTCAACTAAACAGAGGAC | ||
| >520999_BRCA2_19_FOR | ACAAATCAAAGAGAAGCTGCAAGTC | >520999_BRCA2_19_REV | AACCGAGATCACGGGTGACAG | ||
| >520999_BRCA2_20_FOR | CATAAACTGTTTCTATGAGAAAGGTTGTGAG | >520999_BRCA2_20_REV | GACTTTCCAATGTGGTCTTTGCAGC | ||
| >520999_BRCA2_21_FOR | CAGGATTTGGAAAAACATCAGGGAATTC | >520999_BRCA2_21_REV | CTATCTTCTTCAGAGGTATCTACAACTG | ||
| >520999_BRCA2_22_FOR | GTCAATGCCAAATGTCCTAGAAGATGAAG | >520999_BRCA2_22_REV | CTTGGTTTTTAGATTTTTCACATTCATCAGCG | ||
| >520999_BRCA2_23_FOR | CTACAAAAAGTAAGAACTAGCAAGACTAGG | >520999_BRCA2_23_REV | CTTCCACTCTCAAAGGGCTTCTG | ||
| >520999_BRCA2_54_FOR | CCTCAGATGTTATTTTCCAAGCAGG | >520999_BRCA2_54_REV | GTACTCTTCTGCAATATGTAGCTTGG | ||
| >520999_BRCA2_55_FOR | ACTTTCTACTATATTAGAAGAATCAGGAAGTCAG | >520999_BRCA2_55_REV | GACATGAAGATCAGCATCTCTGCATTCC | ||
| >520999_BRCA2_56_FOR | CCTGAAAACCAGATGACTATCTTAAAGACC | >520999_BRCA2_56_REV | CATTTTTCAACAGGCCAGCAAACTTCCG | ||
| >520999_BRCA2_57_FOR | CATCGATTGGTCAGGTAGACAGC | >520999_BRCA2_57_REV | GAAACATTCAGTTTTGTGCCATGAGCAG | ||
| >520999_BRCA2_58_FOR | CAGATGAAAATGAAGTGGGGTTTAGG | >520999_BRCA2_58_REV | GGATGTACCTCTGCAGAAGTTTCCTCAC | ||
| >520999_BRCA2_59_FOR | CTGAATGTTTCTACTGAAGCTCTGC | >520999_BRCA2_59_REV | CACTTACAGTTTTATCATTATGATTTTCTATCTTAAACATTGAAAC | ||
| >520999_BRCA2_60_FOR | GAAACTTCTGCAGAGGTACATCC | >520999_BRCA2_60_REV | CAGTAGTCATTTCAATATTATTTTGTAATATCAGTTGGC | ||
| >520999_BRCA2_61_FOR | CCAATAAGTTTATCTTCAAGTAAATGTCATGATTCTG | >520999_BRCA2_61_REV | CAGTAATTTCTTCAACAAAAGTGCCAGTAGTC | ||
| >520999_BRCA2_62_FOR | GTTTCAATGTTTAAGATAGAAAATCATAATGATAAAACTGTAAGTG | >520999_BRCA2_62_REV | CTAAGTTATGAGAATTTCTACTGGCAGCAG | ||
| >520999_BRCA2_63_FOR | GAAATGACTACTGGCACTTTTGTTGAAG | >520999_BRCA2_63_REV | GCAAGTCCGTTTCATCTTTATGAATACAAAC | ||
| >520999_BRCA2_64_FOR | GTAGAAATTCTCATAACTTAGAATTTGATGGCAGTG | >520999_BRCA2_64_REV | CTTCTTTAATCTGAGTGTTTCCCTCC | ||
| >520999_BRCA2_65_FOR | GCTATTTACTGATCAGCACAACATATGTC | >520999_BRCA2_65_REV | CCATGACATGCTTCTTGAGCTTTCG | ||
| >520999_BRCA2_66_FOR | GGAAACACTCAGATTAAAGAAGATTTGTCAG | >520999_BRCA2_66_REV | GCAGTCTGAAAAAATGTATCAGAAGTCTC | ||
| >520999_BRCA2_67_FOR | GAACAGTTAACTGCTACTAAAACGGAGC | >520999_BRCA2_67_REV | GGAAAAGTTATGCAATTCTTCTGGTTTCTGATC | ||
| >520999_BRCA2_68_FOR | GGGAAAAATATTAGTGTCGCCAAAGAGTC | >520999_BRCA2_68_REV | CTATGTCTGTTTCCTCATAACTTAGAATGTCC | ||
| >520999_BRCA2_69_FOR | GATCAGAAACCAGAAGAATTGCATAACTTTTCC | >520999_BRCA2_69_REV | GATTTCCAGTACCAACTGGGACAC | ||
| >520999_BRCA2_70_FOR | GGACATTCTAAGTTATGAGGAAACAGACATAG | >520999_BRCA2_70_REV | TTCCCGCTAGCTGTATGAAAACC | ||
| >520999_BRCA2_71_FOR | CAGGGACAACCCGAACGTGATG | >520999_BRCA2_71_REV | CTGGTGATTTCACTAGTACCTTGCTC | ||
| >520999_BRCA2_72_FOR | GTTAAAATTGCAAAGGAATCTTTGGACAAAGTG | >520999_BRCA2_72_REV | CTGTGATCTCAATGGTCTCACATGC | ||
| >520999_BRCA2_73_FOR | CCTAAAGTACAGAGAGGCCTG | >520999_BRCA2_73_REV | CTTAGGTGGCACCACAGTCTC | ||
| >520999_BRCA2_74_FOR | CCAAAGTGTAAAGAAATGCAGAATTCTCTC | >520999_BRCA2_74_REV | GACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTAC | ||
| >520999_BRCA2_75_FOR | GTGCCACCTAAGCTCTTAAGTG | >520999_BRCA2_75_REV | GACTGAATAAGGGGACTGATTTGTGTAAC | ||
| >520999_BRCA2_76_FOR | GAAAGTTAAAGTACATGAAAATGTAGAAAAAGAAACAGC | >520999_BRCA2_76_REV | CTTCAAGTAATGAAGTCTGACTCACAGAAG | ||
| >520999_BRCA2_77_FOR | CCTTATTCAGTCATTGAAAATTCAGCCTTAGC | >520999_BRCA2_77_REV | CTGCAGTATTTATTCTTTCTGGTTGACCATC | ||
| >520999_BRCA2_78_FOR | CTGTGAGTCAGACTTCATTACTTGAAGC | >520999_BRCA2_78_REV | GAGATGATTTTTGTCATTTTCAGCTATAGTACTG | ||
| >520999_BRCA2_79_FOR | CCAGAAAGAATAAATACTGCAGATTATGTAGG | >520999_BRCA2_79_REV | CCTCATCAGAATGGTAGGAATAGC | ||
| >520999_BRCA2_80_FOR | CCGAAAAACAAGATACTTATTTAAGTAACAGTAGC | >520999_BRCA2_80_REV | GATCTTCAACATTCTTCAATACTGGCTC | ||
| >520999_BRCA2_81_FOR | CTGATGAGGTATATAATGATTCAGGATATCTCTC | >520999_BRCA2_81_REV | CAGTTTGTGGGTATGCATTTGCATC | ||
| >520999_BRCA2_82_FOR | GAGCCAGTATTGAAGAATGTTGAAGATC | >520999_BRCA2_82_REV | GGTGAAGAGCTAGTCACAAGTTCC | ||
| >520999_BRCA2_83_FOR | GATGCAAATGCATACCCACAAACTG | >520999_BRCA2_83_REV | CGATTTTACCACTGGCTATCCTAAATGCAGG | ||
| >520999_BRCA2_84_FOR | GGAACTTGTGACTAGCTCTTCACCCTGC | >520999_BRCA2_84_REV | GTCTTTCACTTTTTTAATTGTTTCATGTGAAACAC | ||
| >520999_BRCA2_85_FOR | CCTGCATTTAGGATAGCCAGTGG | >520999_BRCA2_85_REV | CAACCTGCCATAATTTTCGTTTGGC | ||
| >520999_BRCA2_86_FOR | CAGTTTCAGTAAAGTAATTAAGGAAAACAACGAG | >520999_BRCA2_86_REV | CCTTATGTGAATGCGTGCTACATTCATC | ||
| >520999_BRCA2_87_FOR | CGAGGCATTGGATGATTCAGAGG | >520999_BRCA2_87_REV | GGTGATATTTTAGAAACTTTCTCCAATCCAGAC | ||
| >520999_BRCA2_88_FOR | GGTTTTTGCTGACATTCAGAGTGAAG | >520999_BRCA2_88_REV | GATGAGACTGACTTATGAAGCTTCCC | ||
| >520999_BRCA2_89_FOR | CCTTGTGATGTTAGTTTGGAAACTTCAG | >520999_BRCA2_89_REV | GCGTTTTGTAATGAAGCATCTGATACC | ||
| >520999_BRCA2_90_FOR | GTGGGATTTTTAGCACAGCAAGTGG | >520999_BRCA2_90_REV | CTCTTGTGAGCTGGTCTGAATGTTC | ||
| >520999_BRCA2_91_FOR | CTGAAATAGAAGATAGTACCAAGCAAGTC | >520999_BRCA2_91_REV | CCAGAGAAAGCAGATGAATTTACCAC | ||
| >520999_BRCA2_92_FOR | GAAAATACTGCTATACGTACTCCAGAAC | >520999_BRCA2_92_REV | CTCTAACACTCCCTTAACTTTGTGTAAGG | ||
| >520999_BRCA2_93_FOR | CAGCAAGTGGAAAGCAAGTTTCC | >520999_BRCA2_93_REV | GGGTTTCTCTTATCAACACGAGG | ||
| >520999_BRCA2_94_FOR | CTTCACTATTCACCTACGTCTAGACA | >520999_BRCA2_94_REV | GTGATTATTTTCTGAAGAACCACCTTCAAC | ||
| >520999_BRCA2_95_FOR | GAGCACTGTGTAAACTCAGAAATGG | >520999_BRCA2_95_REV | CCTAATACCAACTGTTGTTTGTCTTGTTG | ||
| >520999_BRCA2_96_FOR | GAAGGTGGTTCTTCAGAAAATAATCACTC | >520999_BRCA2_96_REV | CGTTTTTAGGTGAAGCCTGTTC | ||
| >520999_BRCA2_97_FOR | GAACCAAAGTGTCACTTGTTGAGAAC | >520999_BRCA2_97_REV | GTAGTTTTCTGAATCTTTGGAGTAAGTAGAAC | ||
| >520999_BRCA2_98_FOR | CTGAAACTTTTTCTGATGTTCCTGTG | >520999_BRCA2_98_REV | GAATGTGTGGCATGACTTGGCAG | ||
| >520999_BRCA2_99_FOR | GCTTTTATGGAAGATGATGAACTGAC | >520999_BRCA2_99_REV | AAATGAACACTTACCCACTAAGATAAGG | ||
| >520999_BRCA2_100_FOR | CATGTCCCGAAAATGAGGAAATGG | >520999_BRCA2_100_REV | CACTTTAAAAATAGTGATTGGCAACACGAAAGG | ||
| >520999_BRCA2_101_FOR | GGTCTATAGACTTTTGAGAAATAAAACTGATATTATTTGCC | >520999_BRCA2_101_REV | TGGAGTGCTTTTTGAAGCCTTTAAG | ||
| >520999_BRCA2_102_FOR | CCTCAATCAAAAGAAACTTATTAAATGAATTTGACAGG | >520999_BRCA2_102_REV | CAGAATATTATATACCATACCTATAGAGGGAGAACAG | ||
| >520999_BRCA2_103_FOR | ATTGTCTCAAATTTTTTGTGTATTTACAGTAACA | >520999_BRCA2_103_REV | GGTAATCGGCTCTAAAGAAACATGATGC | ||
| >520999_BRCA2_104_FOR | CTAATATGTAATATAAAATAATTGTTTCCTAGGCAC | >520999_BRCA2_104_REV | AACGAGACTTTTCTCATACTGTATTAGAATTTAG | ||
| >520999_BRCA2_105_FOR | ATGTAGCAAATGAGGGTCTGCAACAA | >520999_BRCA2_105_REV | GACAGAAATTCTTGACCAGGTGCG | ||
| >520999_BRCA2_106_FOR | ACTAAGGAACGTCAAGAGATACAGAATCC | >520999_BRCA2_106_REV | GAAACTTGATAAAATGGATGTCCTGAAACTGC | ||
| >520999_BRCA2_107_FOR | CTAAATCTCATTTGTATGAACATCTGACTTTGG | >520999_BRCA2_107_REV | GGAACAAAGACTTTGGTTGGTCTGCCTGTAG | ||
| >520999_BRCA2_108_FOR | GTTTCTGCTACAAGAAATGAAAAAATGAGACAC | >520999_BRCA2_108_REV | CAGAGCCATGTCCATCAATGTTTTGC | ||
| >520999_BRCA2_109_FOR | TCACAGAGTTGAACAGTGTGTTAGGAATAT | >520999_BRCA2_109_REV | CAGCTACTGCTTGATTGGAGTTG | ||
| >520999_BRCA2_110_FOR | GCTCTGATGATAGTAAAAATAAGATTAATGACAATGAG | >520999_BRCA2_110_REV | GACATTATTTAACAACGGAAATATCTAACTGAAAGGC | ||
| >520999_BRCA2_111_FOR | GGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAG | >520999_BRCA2_111_REV | CAGACTGCCTGGCTGTGGAAAGACG | ||
| >520999_BRCA2_112_FOR | GATTTAATTACAAGTCTTCAGAATGCCAGAG | >520999_BRCA2_112_REV | GATTCGAGGCAGAGTGGATGTTTTT | ||
| >520999_BRCA2_113_FOR | AACAAAGGCAACGCGTCTTTCC | >520999_BRCA2_113_REV | CCATCAGTATTGTAGACAAACACATACCTG | ||
| >520999_BRCA2_114_FOR | GCCAAGTTCCCTCTGCGTGTT | >520999_BRCA2_114_REV | TGTGTTCATTCTTTAAACAAAGCCATTTGT | ||
| >520999_BRCA2_115_FOR | TCAGTTTTGGTTTGTTATAATTGTTTTTATTGTGT | >520999_BRCA2_115_REV | AGACTCTGCATTTTTGCTGTTAATTTTTATGCAA | ||
| >520999_BRCA2_116_FOR | TTTATTTTGTGTAGCTGTATACGTATGGCG | >520999_BRCA2_116_REV | CCATCCACCATCAGCCAACTGTATT | ||
| >520999_BRCA2_117_FOR | AAGATTATTTTGGTAAGGAAAGTTTATGGACTGG | >520999_BRCA2_117_REV | GGGATGAGGGAATACATAAAAGTTAACAC | ||
| >520999_BRCA2_118_FOR | GTTGTAGTTGTTGAATTCAGTATCATCCTATGTGG | >520999_BRCA2_118_REV | GCTGCCAGTTTCCATATGATCCATCTATAGTG | ||
| >520999_BRCA2_119_FOR | TTATTTGTTCAGGGCTCTGTGTGACACTCC | >520999_BRCA2_119_REV | GGGCTTAGGCATCTATTAGCAAATTCC | ||
| >520999_BRCA2_120_FOR | TATAGATGGATCATATGGAAACTGGCAGC | >520999_BRCA2_120_REV | CCGTATATGATTACGTAATGTAATGCTTTAAACT | ||
| >520999_BRCA2_121_FOR | TGCTAATAGATGCCTAAGCCCAGA | >520999_BRCA2_121_REV | CTGGCTTGTGCAACATTTTGACATGG | ||
| >520999_BRCA2_122_FOR | AGTTTTTATTCTCAGTTATTCAGTGACTTGTT | >520999_BRCA2_122_REV | CATTATCTTTTTTATAGCCGATCTTCTGCTTC | ||
| >520999_BRCA2_123_FOR | GCATTTTTGTTTTCACTTTTAGATATGATACGG | >520999_BRCA2_123_REV | GCTAGAAGTTTCAGATATATTTGCGCTC | ||
| >520999_BRCA2_124_FOR | GGATGACACAGCTGCAAAAACAC | >520999_BRCA2_124_REV | CAGCATACCACCCATCTGTAAGTTC | ||
| >520999_BRCA2_125_FOR | GCAATAAAACTAGTAGTGCAGATACCCA | >520999_BRCA2_125_REV | CTGCTCCATGAAGAATAATCTTCTGACC | ||
| >520999_BRCA2_126_FOR | GCCCAGTTAGATCCTCCCCTCT | >520999_BRCA2_126_REV | ACCTTTAACATAAGAGATTCTGGGGC | ||
| >520999_BRCA2_127_FOR | GGGCTCTCCTGATGCCTGTAC | >520999_BRCA2_127_REV | AAATTGAGCATCCTTAGTAAGCATTTAAAATTTAA | ||
| >520999_BRCA2_128_FOR | GCAGTTCTAGAAGAATGAAAACTCTTATGATATCTG | >520999_BRCA2_128_REV | GGAAAGAATCCAAGTTTGGTATACCAGCGAG | ||
| >520999_BRCA2_129_FOR | TATTAATTTGTCCAGATTTCTGCTAACAGTACTC | >520999_BRCA2_129_REV | CATCATACCTGTATAGGGTATGCTC | ||
| >520999_BRCA2_130_FOR | CCTTATCATCGCTTTTCAGTGATGGAG | >520999_BRCA2_130_REV | GAGACCGAAACTCCATCTCAAACAAAC | ||
| >520999_BRCA2_131_FOR | GAATGTTATATATGTGACTTTTTTGGTGTGTGTAACAC | >520999_BRCA2_131_REV | CAAATTCCTCCTGAATTTTAGTGAATAAGGCTTC | ||
| >520999_BRCA2_132_FOR | CAATGAAAGAGAGGAAGAAAAGGAAGCAGC | >520999_BRCA2_132_REV | CTTTGTTCTCATATTAGAAATAACAATGTGTACC | ||
| >520999_BRCA2_133_FOR | CCTTCTTTGGGTGTTTTATGCTTGG | >520999_BRCA2_133_REV | GCACGAACTTGCTGTCTTGTTAG | ||
| >520999_BRCA2_134_FOR | CTTAGAAAACACAACAAAACCATATTTACCATC | >520999_BRCA2_134_REV | CATCAAGCCTCATTATATGTCCTCTTACTC | ||
| >520999_BRCA2_135_FOR | GTTCGTGCTTTGCAAGATGGTG | >520999_BRCA2_135_REV | GGCCAGAGAGTCTAAAACAGCTTC | ||
| >520999_BRCA2_136_FOR | CTAGTTACAATAGATGGAACTTTTTTGTTCTGATTGC | >520999_BRCA2_136_REV | GAGCTTGTTTCTTATCATTCAACATTTGCC | ||
| >520999_BRCA2_137_FOR | GTCACAGGGTTATTTCAGTGAAGAGC | >520999_BRCA2_137_REV | GTGACATCCCTTGATAAACCTTGTTCC | ||
| >520999_BRCA2_138_FOR | AAGCTCAGATCCAGTTGGAAATTAGGAAG | >520999_BRCA2_138_REV | CTGATAAAAACAAAGCATTTACATACTTACCTG | ||
| >520999_BRCA2_139_FOR | GTTTATCAAGGGATGTCACAACCGT | >520999_BRCA2_139_REV | TCTGGATTTATAATCATTTTGTTAGTAAGGTCATT | ||
| >520999_BRCA2_140_FOR | AAAAGAGGATCTGTATTTATTTTGAAACAAACAT | >520999_BRCA2_140_REV | GCAAGATGATAAATTCTGTATCTCTTTCCTTCTG | ||
| >520999_BRCA2_141_FOR | GGCGTCCATCATCAGATTTATATTCTCTG | >520999_BRCA2_141_REV | TGTACCGGTAGTTGTTGATACTGAG | ||
| >520999_BRCA2_142_FOR | AAAGTAAATCTGAAAGAGCTAACATACAGTTAGC | >520999_BRCA2_142_REV | TGAAACCTACAGAAAACAAAACAAAAATTCAAC | ||
| >520999_BRCA2_143_FOR | TCAGTTTTGATAAGTGCTTGTTAGTTTATGG | >520999_BRCA2_143_REV | CAAGATGGCTGAAAGTCTGGATCTAAA | ||
| >520999_BRCA2_144_FOR | AAATTTTATTTCAGATTTACCAGCCACGG | >520999_BRCA2_144_REV | GTGCATTACCTGTTTTTTTCACAACAG | ||
| >520999_BRCA2_145_FOR | CCATCTTGTTCTGAGGTGGACCTAA | >520999_BRCA2_145_REV | GCCAACTGGTAGCTCCAACTAATCA | ||
| >520999_BRCA2_146_FOR | TAGGCATATTAGAGTTTCCTTTCTTGCATC | >520999_BRCA2_146_REV | AGGTCTATCCAAAACTTTATTGCCAGT | ||
| >520999_BRCA2_147_FOR | ATTCTAGGACTTGCCCCTTTCGTC | >520999_BRCA2_147_REV | ATTTGGATTCTGGTCGCCACTGGA | ||
| >520999_BRCA2_148_FOR | GACCTTAATGAGGACATTATTAAGCCTCA | >520999_BRCA2_148_REV | TGTCTCTTGAAAGTGGCCCTCTTT | ||
| >520999_BRCA2_149_FOR | TCAGGCCTTCTTACTTTATTTGCTGG | >520999_BRCA2_149_REV | GAAAAATACCAAAATGTGTGGTGATGCTG | ||
| >520999_BRCA2_150_FOR | GGTCCAAACTTTTCATTTCTGCTTTTAAAGG | >520999_BRCA2_150_REV | TTGCATGCAGTATATGCATAAGCTTGT | ||
| >520999_BRCA2_151_FOR | TTTTCTTAGAATATTGACATACTTTGCAATGAAGC | >520999_BRCA2_151_REV | ACCAGAAGCTTGTTTCCTGTACC | ||
| >520999_BRCA2_152_FOR | TCCACCCCAACTAAAGACTGTACT | >520999_BRCA2_152_REV | CTAGGTATACAACAGAATATACGATGGCC | ||
| >520999_BRCA2_153_FOR | TTGCGTGCTTAAATATTTTCAATGAAAAGTTACTT | >520999_BRCA2_153_REV | CAGGTGTGGAAACAGACTTCCTTT | ||
| >520999_BRCA2_154_FOR | CTTCTCCTAATTGTGAGATATATTATCAAAGTCC | >520999_BRCA2_154_REV | AGGCTCTTCTCTTTTTGCAGTTCTT | ||
| >520999_BRCA2_155_FOR | TCAGCCCAGATGACTTCAAAGTCT | >520999_BRCA2_155_REV | GCAGCCGGAGAAACAAATGTACAA | ||
| >520999_BRCA2_156_FOR | TCTTGAGTAGACTGCCTTTACCTCCA | >520999_BRCA2_156_REV | GGAGTCATCTGAGGAGAATTCAGTTCT | ||
| >520999_BRCA2_157_FOR | ATTTCAGCCACCAAGGAGTTGTG | >520999_BRCA2_157_REV | CAATGCAAGTTCTTCGTCAGCTATTG | ||
| >520999_BRCA2_158_FOR | CTCAGATGACTCCATTTAAAAAATTCAATGAAATT | >520999_BRCA2_158_REV | GAGCAGTCCTAGTGGATTCACTGA | ||
| >520999_BRCA2_159_FOR | CAAGCTCTTTTGTCTGGTTCAACAGG | >520999_BRCA2_159_REV | GCCTGGGAACTCTCCTGTTCTTT | ||
| >520999_BRCA2_160_FOR | CTCAGACTGAAACGACGTTGTACTACATC | >520999_BRCA2_160_REV | CCAGTCTTATAAACTGGAAAGGTTAAGCGTC | ||
| >520999_BRCA2_161_FOR | GTACGGAAGAATGTGAGAAAAATAAGCAGG | >520999_BRCA2_161_REV | TGTGCAACATAAGTACTAATGTGTGGT | ||
| >520999_AKT1_01_FOR | GAGTGAGGATGGCTACAGGCAGAGGTG | >520999_AKT1_01_REV | CCTGCAGTGGACCACTGTCATCGAAC | ||
| >520999_AKT1_02_FOR | CACGTACCGCTCCTCAGGAGTCTCCAC | >520999_AKT1_02_REV | AGTGCCAGCTGATGAAGACGGAGCG | ||
| >520999_AKT1_03_FOR | GCAGGCAGCGGATGATGAAGGTGTTG | >520999_AKT1_03_REV | GGTATGCAAGGGGAGCTGGCTGGTG | ||
| >520999_AKT1_04_FOR | GCTCCAGCCAACCCCCCAAATCTGAAT | >520999_AKT1_04_REV | CTCAAGAATGATGGCACCTTCATTGGCTAC | ||
| >520999_AKT1_05_FOR | ACAGAGAAGTTGTTGAGGGGAGCCTCA | >520999_AKT1_05_REV | GCACGTCTGTAGGGGAGTACATCAAGAC | ||
| >520999_AKT1_06_FOR | GTGCCATCATTCTTGAGGAGGAAGTAGCGTGG | >520999_AKT1_06_REV | CCTGGATGACTGGCGAGGGTCTGACG | ||
| >520999_TP53_01_FOR | TCTGACGCACACCTATTGCAAGCAAG | >520999_TP53_01_REV | GGTCAGTCTACCTCCCGCCATAAAAAAC | ||
| >520999_TP53_02_FOR | GTGGGGAACAAGAAGTGGAGAATGTCAG | >520999_TP53_02_REV | CACAGACCCTCTCACTCATGTGATGT | ||
| >520999_TP53_03_FOR | TCAGCTGCCTTTGACCATGAAGGCA | >520999_TP53_03_REV | TGAATGAGGCCTTGGAACTCAAGGATGC | ||
| >520999_TP53_04_FOR | CTGAGGTCACTCACCTGGAGTGAG | >520999_TP53_04_REV | CTCCTCTGTTGCTGCAGATCCG | ||
| >520999_TP53_05_FOR | GGCATCCTTGAGTTCCAAGGCCTCA | >520999_TP53_05_REV | GCATGTTGCTTTTGTACCGTCATAAAGTC | ||
| >520999_TP53_06_FOR | GCAGGCTAGGCTAAGCTATGATGTTCC | >520999_TP53_06_REV | GCATGAAAATGGTTCTATGACTTTGCCTG | ||
| >520999_TP53_07_FOR | GGAAGTAACACCATCGTAAGTCAAG | >520999_TP53_07_REV | GTAGCTAACTAACTTCAGAACACCAACTT | ||
| >520999_TP53_08_FOR | ACGGCATTTTGAGTGTTAGACTGGA | >520999_TP53_08_REV | CTTTCCTAGCACTGCCCAACAACAC | ||
| >520999_TP53_09_FOR | CATCCAGTGGTTTCTTCTTTGGCTG | >520999_TP53_09_REV | GCACTAAGCGAGGTAAGCAAGCAGG | ||
| >520999_TP53_10_FOR | GGCATAACTGCACCCTTGGTCTCCTCCAC | >520999_TP53_10_REV | GGAACAGCTTTGAGGTGCGTGTTTGT | ||
| >520999_TP53_11_FOR | CCTTTCTTGCGGAGATTCTCTTCCTC | >520999_TP53_11_REV | GTAGGACCTGATTTCCTTACTGCCTC | ||
| >520999_TP53_12_FOR | GATGAGAGGTGGATGGGTAGTAGTATGG | >520999_TP53_12_REV | CCATCCTCACCATCATCACACTGGAA | ||
| >520999_TP53_13_FOR | CAGTGTGCAGGGTGGCAAGTGGCTC | >520999_TP53_13_REV | ATCTCCTAGGTTGGCTCTGACTGTAC | ||
| >520999_TP53_14_FOR | GGTTCATGCCGCCCATGCAGGAACTG | >520999_TP53_14_REV | CCTGCTTGCCACAGGTCTCCCCAAGG | ||
| >520999_TP53_15_FOR | ACTGACAACCACCCTTAACCCCTCCT | >520999_TP53_15_REV | CCCTCCTCAGCATCTTATCCGAGTG | ||
| >520999_TP53_16_FOR | CTGTCATCCAAATACTCCACACGCA | >520999_TP53_16_REV | CTCAGATAGCGATGGTGAGCAGCTG | ||
| >520999_TP53_17_FOR | GACCCTGGGCAACCAGCCCTGTC | >520999_TP53_17_REV | CGCGCCATGGCCATCTACAAGCAG | ||
| >520999_TP53_18_FOR | GGCAGCGCCTCACAACCTCCGTC | >520999_TP53_18_REV | CCCTGCCCTCAACAAGATGTTTTGCC | ||
| >520999_TP53_19_FOR | TGTGGAATCAACCCACAGCTGCACA | >520999_TP53_19_REV | TGCCGTCTTCCAGTTGCTTTATCTG | ||
| >520999_TP53_20_FOR | GATACGGCCAGGCATTGAAGTCTC | >520999_TP53_20_REV | GTCCCTTCCCAGAAAACCTACCAG | ||
| >520999_TP53_21_FOR | CAGAATGCAAGAAGCCCAGACGGAAAC | >520999_TP53_21_REV | AGATGAAGCTCCCAGAATGCCAGAGG | ||
| >520999_TP53_22_FOR | GGTGTAGGAGCTGCTGGTGCAGG | >520999_TP53_22_REV | CCTTGCCGTCCCAAGCAATGGATG | ||
| >520999_TP53_23_FOR | GTCTTCAGTGAACCATTGTTCAATATCGTCC | >520999_TP53_23_REV | TCTGACTGCTCTTTTCACCCATCTAC | ||
| >520999_TP53_24_FOR | CATCAAATCATCCATTGCTTGGGAC | >520999_TP53_24_REV | CTGACTTTCTGCTCTTGTCTTTCAGACTTC | ||
| >520999_TP53_25_FOR | CCCAACCCTTGTCCTTACCAGAACG | >520999_TP53_25_REV | CCATTGGAAGGGCAGGCCCAC | ||
| >520999_TP53_26_FOR | CAAGAGCAGAAAGTCAGTCCCATGG | >520999_TP53_26_REV | GTCGAGCCCCCTCTGAGTCAG | ||
| >520999_TP53_27_FOR | GGATCCACTCACAGTTTCCATAGGTCTG | >520999_TP53_27_REV | GATCCCCACTTTTCCTCTTGCAGCAG | ||
| >520999_ERBB2_01_FOR | TGTGGTCTCCCATACCCTCTCAGCGTAC | >520999_ERBB2_01_REV | GGTCTAAGAGGCAGCCATAGGGCATAAGC | ||
| >520999_ERBB2_02_FOR | CATCTGCCTGACATCCACGGTGCAG | >520999_ERBB2_02_REV | GGACATAGGGGTTTGCTCCGGAGAGAC | ||
| >520999_BRCA1_01_FOR | GATTTCCAAGGGAGACTTCAAGCAG | >520999_BRCA1_01_REV | GAGCTTACAAAGTGGCCTTTCC | ||
| >520999_BRCA1_02_FOR | AGGACAGTAGAAGGACTGAAGAGTGAGAGG | >520999_BRCA1_02_REV | GATACCCCAGATCCCCCACAGCCACTACTGAC | ||
| >520999_BRCA1_03_FOR | TCCTGTGGCTCTGTACCTGTG | >520999_BRCA1_03_REV | ATGTGTGAGGCACCTGTGGTGA | ||
| >520999_BRCA1_04_FOR | TCCTGGCACTGGTAGAGTGCT | >520999_BRCA1_04_REV | CGATTGATTAGAGCCTAGTCCAGGAG | ||
| >520999_BRCA1_05_FOR | CAAGAACTGTGCTACTCAAGCACC | >520999_BRCA1_05_REV | CAATTGTGGTTGTGCAGCCAGA | ||
| >520999_BRCA1_06_FOR | ACAGGTACATGCAGGCACCTTAC | >520999_BRCA1_06_REV | GAAGTGACAGTTCCAGTAGTCCTAC | ||
| >520999_BRCA1_07_FOR | AGGCTACAGTAGGGGCATCCATA | >520999_BRCA1_07_REV | TGCTTCTGTGGTGAAGGAGCT | ||
| >520999_BRCA1_08_FOR | ACAGGGCACCCAATACTTACTGTG | >520999_BRCA1_08_REV | CCTGGGTTAAGTATGCAGATTACTGC | ||
| >520999_BRCA1_09_FOR | AGCAATCTGAGGAACCCCCAT | >520999_BRCA1_09_REV | GGCTAGAAATCTGTTGCTATGGGC | ||
| >520999_BRCA1_10_FOR | TGCTGGAACTCTGGGGTTCTC | >520999_BRCA1_10_REV | ACTAGATTTTCCTTCTCTCCATTCCCC | ||
| >520999_BRCA1_11_FOR | CAAAATGAAGCGGCCCATCTCT | >520999_BRCA1_11_REV | GGTCAATGGAAGAAACCACCAAGG | ||
| >520999_BRCA1_12_FOR | CTTGAGGGAGGGAGCTTTACCTTT | >520999_BRCA1_12_REV | AGCTTCTCTTTCTCTTATCCTGATGGG | ||
| >520999_BRCA1_13_FOR | CATTGACCACATCTCCTCTGACTTC | >520999_BRCA1_13_REV | CGCCTGGCCTGAATGCCTTAA | ||
| >520999_BRCA1_14_FOR | AGGAAAGTGGTGCATTGATGGAAG | >520999_BRCA1_14_REV | TGATCTCTTTAGGGGTGACCCAG | ||
| >520999_BRCA1_15_FOR | GTAACATCAAGTACTTACCTCATTCAGCA | >520999_BRCA1_15_REV | CTGTGCTCTTTTGTGAATCGCTGA | ||
| >520999_BRCA1_16_FOR | GTTAGGTGTAAAAATGCAATTCTGAGGTG | >520999_BRCA1_16_REV | GGACACTGAAATATTTTCTAGGAATTGCG | ||
| >520999_BRCA1_17_FOR | GAGGGGAGAAATAGTATTATACTTACAGAAATAGC | >520999_BRCA1_17_REV | CAGCACTTCCTGATTTTGTTTTCAACT | ||
| >520999_BRCA1_18_FOR | GCAGCAGATGCAAGGTATTCTGTAAAGG | >520999_BRCA1_18_REV | GATTTAATTTCAGATGCTCGTGTACAAGTTTGCC | ||
| >520999_BRCA1_19_FOR | CATGAGTAGTCTCTTCAGTAATTAGATTAG | >520999_BRCA1_19_REV | GTATTCTGAGCTGTGTGCTAGAGGTAACTC | ||
| >520999_BRCA1_20_FOR | TCTTCCTCTAGGTTATTAATTGACAATACCTAC | >520999_BRCA1_20_REV | CAACAGAAAGGGTCAACAAAAGAATGT | ||
| >520999_BRCA1_21_FOR | CACTCACAAATTCTTCTGGGGTCAG | >520999_BRCA1_21_REV | GCTGCTGCTCATACTACTGATACTGC | ||
| >520999_BRCA1_22_FOR | CTTCTCCCTGCTCACACTTTCTTC | >520999_BRCA1_22_REV | GCTCGTGTTGGCAACATACCATC | ||
| >520999_BRCA1_23_FOR | CTGGGCAGATTCTGCAACTTTC | >520999_BRCA1_23_REV | GGAATCTGGAATCAGCCTCTTCTCTG | ||
| >520999_BRCA1_24_FOR | TCTGGGGCTCTGTCTTCAGAAG | >520999_BRCA1_24_REV | ACAGAGACCAGAACTTTGTAATTCAACA | ||
| >520999_BRCA1_25_FOR | AGACTAGTACATCTAAAAGTTGGTTAACCAG | >520999_BRCA1_25_REV | AGTCTGGGCCACACGATTTGA | ||
| >520999_BRCA1_26_FOR | GATGAAATATTACCTAGATCTTGCCTTGGC | >520999_BRCA1_26_REV | AGTTGCTCTGGGAGTCTTCAGAATA | ||
| >520999_BRCA1_27_FOR | CACATCAACAACCTTAATGAGCTCCTC | >520999_BRCA1_27_REV | GTGGCGATGGTTTTCTCCTTCCATT | ||
| >520999_BRCA1_28_FOR | GTACCACCTATCATCTAATGATGGGC | >520999_BRCA1_28_REV | GCTGTTTCATTTATGCTTTGGCTGCC | ||
| >520999_BRCA1_29_FOR | GATGTCAGATACCACAGCATCTTTACATTGATG | >520999_BRCA1_29_REV | GAATACCCTATAAGCCAGAATCCAGAAGG | ||
| >520999_BRCA1_30_FOR | GAACTATCTGCAGACACCTCAAACTTGTC | >520999_BRCA1_30_REV | CTAACCTGAATTATCACTATCAGAACAAAGCAG | ||
| >520999_BRCA1_31_FOR | AGCAGAAAGGCCTTCTGGATTCT | >520999_BRCA1_31_REV | GATGCTTTTGAAAAAAATCAGTATTCTAACCTG | ||
| >520999_BRCA1_32_FOR | GCAGAGGGAAGGCTCAGATACAAACACAGC | >520999_BRCA1_32_REV | CTAAAAGGCCTAGAGAACATATATCAGG | ||
| >520999_BRCA1_33_FOR | ACCATCTTTCAGTAATTTGCCAAAATGACG | >520999_BRCA1_33_REV | GAACAGTACCCGTTCCCTTGATGTCTACAATTTC | ||
| >520999_BRCA1_34_FOR | GAGCTAGGTCCTTACTCTTCAGAAGG | >520999_BRCA1_34_REV | CTACCCTTCCATCATAAGTGACTC | ||
| >520999_BRCA1_35_FOR | ACCTTTTTCTGATGTGCTTTGTTCTGG | >520999_BRCA1_35_REV | ACCTGATAAAGCTCCAGCAGGAAAT | ||
| >520999_BRCA1_36_FOR | TAGCTGTTAGAAGGCTGGCTCC | >520999_BRCA1_36_REV | GGAAAGCTTCTCAAAGTATTTCATTTTCTTGG | ||
| >520999_BRCA1_37_FOR | GTCAGCAAACCTAAGAATGTGGGA | >520999_BRCA1_37_REV | AGCGTCTCTGAAGACTGCTCA | ||
| >520999_BRCA1_38_FOR | CACACACACGCTTTTTACCTGAGT | >520999_BRCA1_38_REV | AGTCTGCTTTTACATCTGAACCTCTG | ||
| >520999_BRCA1_39_FOR | AGACGCTTGTTTCACTCTCACAC | >520999_BRCA1_39_REV | GAAAGTAAATCCAGTCCTGCCAATGAG | ||
| >520999_BRCA1_40_FOR | TTGTAAAATGTGCTCCCCAAAAGC | >520999_BRCA1_40_REV | AAGAGGAACGGGCTTGGAAGA | ||
| >520999_BRCA1_41_FOR | CCTGGTTCCAATACCTAAGTTTGAATCC | >520999_BRCA1_41_REV | GGTTCTTCCAAACAAATGAGGCATCAGT | ||
| >520999_BRCA1_42_FOR | AAACCAATTCCTTGTCACTCAGACC | >520999_BRCA1_42_REV | CTGCTAGCTTGTTTTCTTCACAGTG | ||
| >520999_BRCA1_43_FOR | AGGATCCTGGGTGTTTGTATTTGC | >520999_BRCA1_43_REV | GCTTAAATGACTGCAGTAACCAGGT | ||
| >520999_BRCA1_44_FOR | CTCACTAAGGTGATGTTCCTGAGATGC | >520999_BRCA1_44_REV | CACTTGTTATTTGGTAAAGTAAACAATATACCTTCTCAGTCTACT | ||
| >520999_BRCA1_45_FOR | TTAGACAGACACTCGGTAGCAACG | >520999_BRCA1_45_REV | GGGCCAAGAAATTAGAGTCCTCAG | ||
| >520999_BRCA1_46_FOR | AACAAGTGTTGGAAGCAGGGAAG | >520999_BRCA1_46_REV | CAGAAAGGAGAGCTTAGCAGGAGT | ||
| >520999_BRCA1_47_FOR | GGTAACCCTGAGCCAAATGTGTATG | >520999_BRCA1_47_REV | CACCTGATGACCTGTTAGATGATGGTG | ||
| >520999_BRCA1_48_FOR | TGCTAAAAACAGCAGAACTTTCCTTAATGTCATTTTCAGCA | >520999_BRCA1_48_REV | AGAAGTAGTTCAGACTGTTAATACAGATTTCTCTCCA | ||
| >520999_BRCA1_49_FOR | CTTCCCATAGGCTGTTCTAAGTTATCTGA | >520999_BRCA1_49_REV | GGTTTTGCAACCTGAGGTCTATAAACA | ||
| >520999_BRCA1_50_FOR | TTTTATTTCAGGATGCTTACAATTACTTCCAGG | >520999_BRCA1_50_REV | TCCAGTATTAATGAAATAGGTTCCAGTGA | ||
| >520999_BRCA1_51_FOR | GGTTGCAAAACCCCTAATCTAAGCATAG | >520999_BRCA1_51_REV | GCCAGCTCAAGCAATATTAATGAAGTAGG | ||
| >520999_BRCA1_52_FOR | TGGAGCCCACTTCATTAGTACTGG | >520999_BRCA1_52_REV | CAATGTCACCTGAAAGAGAAATGGGA | ||
| >520999_BRCA1_53_FOR | TACGGCTAATTGTGCTCACTGTAC | >520999_BRCA1_53_REV | ACCACCACTTTTTCCCATCAAGTC | ||
| >520999_BRCA1_54_FOR | TGTTCCTCAAAGTTTTCCTCTAGCAG | >520999_BRCA1_54_REV | AGTTCAGAGGCAACGAAACTGG | ||
| >520999_BRCA1_55_FOR | AGTGGTGGTATACGATATGGGTTTTGT | >520999_BRCA1_55_REV | GGTTGGTCAGAAAGATAAGCCAGTTGATA | ||
| >520999_BRCA1_56_FOR | AACTGAGATGATAGACAAAACCTAGAGCC | >520999_BRCA1_56_REV | CAAAAGGAAGAAAATCAAGGAAAGAATGAGTC | ||
| >520999_BRCA1_57_FOR | CCACAGGAAAGCCTGCAGTGATA | >520999_BRCA1_57_REV | AACATTCTCTGCCCACTCTGGGT | ||
| >520999_BRCA1_58_FOR | TTGTTCACATTCAAAAGTGACTTTTGGAC | >520999_BRCA1_58_REV | TGCTCAGTATTTGCAGAATACATTCAAGG | ||
| >520999_BRCA1_59_FOR | GCACATTCCTCTTCTGCATTTCCTGG | >520999_BRCA1_59_REV | GAAGGCTTTAAGTATCCATTGGGACATG | ||
| >520999_BRCA1_60_FOR | TCTTCCATTTCTATGCTTGTTTCCCG | >520999_BRCA1_60_REV | ATGTGTGAGTCAGTGTGCAGCA | ||
| >520999_BRCA1_61_FOR | CTTTGGAACAACCATGAATTAGTCCC | >520999_BRCA1_61_REV | TGGTACCTGGTACTGATTATGGCA | ||
| >520999_BRCA1_62_FOR | CTTCCCTAGAGTGCTAACTTCCAGT | >520999_BRCA1_62_REV | TGCTGAAGACCCCAAAGATCTCATG | ||
| >520999_BRCA1_63_FOR | AATACTGCTACTCTCTACAGATCTTTCAGT | >520999_BRCA1_63_REV | TGAACTTAAAGAATTTGTCAATCCTAGCCTT | ||
| >520999_BRCA1_64_FOR | TCAGCATTATTAGACACTTTAACTGTTTCTAGTTTCTCT | >520999_BRCA1_64_REV | CAAGCCAAATGAACAGACAAGTAAAAGAC | ||
| >520999_BRCA1_65_FOR | TGCATTTGTTAACTTCAGCTCTGGG | >520999_BRCA1_65_REV | AAATGCCAGTCAGGCACAGCA | ||
| >520999_BRCA1_66_FOR | CTTCTTGGCTCCAGTTGCAGGT | >520999_BRCA1_66_REV | AGCCCACCTAATTGTACTGAATTGC | ||
| >520999_BRCA1_67_FOR | TGTACTTTTTTTTCTTTATCTCTTCACTGCTAG | >520999_BRCA1_67_REV | TCAAAAGCACCTAAAAAGAATAGGCTG | ||
| >520999_BRCA1_68_FOR | ACTAGTTCAAGCGCATGAATATGCC | >520999_BRCA1_68_REV | CTGCTTTCAAAACGAAAGCTGAACC | ||
| >520999_BRCA1_69_FOR | ATTTAATTCGAGTTCCATATTGCTTATACTGCTG | >520999_BRCA1_69_REV | CAAGTGATGAATATTACTAATAGTGGTCATGAGA | ||
| >520999_BRCA1_70_FOR | GTTAGGATTTTTCTCATTCTGAATAGAATCACC | >520999_BRCA1_70_REV | GGATTTTATCAAGAAAGCAGATTTGGCAGT | ||
| >520999_BRCA1_71_FOR | TGCTCCGTTTGGTTAGTTCCCT | >520999_BRCA1_71_REV | ACAAGAGCGTCCCCTCACAAAT | ||
| >520999_BRCA1_72_FOR | AAATCCTCAGGATGAAGGCCTGA | >520999_BRCA1_72_REV | AAGCCTCCCCAACTTAAGCCA | ||
| >520999_BRCA1_73_FOR | CTGTGGCTCAGTAACAAATGCTCCTA | >520999_BRCA1_73_REV | GCTTTAATATGTAAAAGTGAAAGAGTTCACTCC | ||
| >520999_BRCA1_74_FOR | CTTCCGATAGGTTTTCCCAAATATTTTGTC | >520999_BRCA1_74_REV | GACGTTCTAAATGAGGTAGATGAATATTCTGG | ||
| >520999_BRCA1_75_FOR | AAGCCTCATGAGGATCACTGGCCAGTAAGTC | >520999_BRCA1_75_REV | GTGATGAACTGTTAGGTTCTGATGACTC | ||
| >520999_BRCA1_76_FOR | CGTCCAATACATCAGCTACTTTGGC | >520999_BRCA1_76_REV | CCTAGAGATACTGAAGATGTTCCTTGG | ||
| >520999_BRCA1_77_FOR | ACCACTCATTAACTTTCTGAATGCTGC | >520999_BRCA1_77_REV | CCCAGCACAGAAAAAAAGGTAGATCTG | ||
| >520999_BRCA1_78_FOR | CTTATTCCATTCTTTTCTCTCACACAGG | >520999_BRCA1_78_REV | GGCTTAGCAAGGAGCCAACATAAC | ||
| >520999_BRCA1_79_FOR | TGGGAGTCCGCCTATCATTACATGT | >520999_BRCA1_79_REV | CAGCATGAGAACAGCAGTTTATTACTCAC | ||
| >520999_BRCA1_80_FOR | TATGTTGGCTCCTTGCTAAGCCAG | >520999_BRCA1_80_REV | TGTGGAGCCATGTGGCACAAAT | ||
| >520999_BRCA1_81_FOR | TGAGTAATAAACTGCTGTTCTCATGCTGTAATGAGCT | >520999_BRCA1_81_REV | GGATGTAACAAATACTGAACATCATCAACCCAGT | ||
| >520999_BRCA1_82_FOR | CTGCACGCTTCTCAGTGGTGTTCA | >520999_BRCA1_82_REV | TGATTTCCACCTCCAAGGTGTATGAAGTATGT | ||
| >520999_BRCA1_83_FOR | GTTACATCCGTCTCAGAAAATTCACAAG | >520999_BRCA1_83_REV | TAGCCAGTTGGTTGATTTCCACC | ||
| >520999_BRCA1_84_FOR | GTAAAGGTCCCAAATGGTCTTCAGA | >520999_BRCA1_84_REV | ACCCCTCAAGGAACCAGGGATG | ||
| >520999_BRCA1_85_FOR | ACTTTGCCATTACCCTTTTTTGCAGA | >520999_BRCA1_85_REV | GGTCATTTGACAGTTCTGCATACATGTAAC | ||
| >520999_BRCA1_86_FOR | CCAGCTTCATAGACAAAGGTTCTC | >520999_BRCA1_86_REV | GAAAACTTTTATTGATTTATTTTTTGGGG | ||
| >520999_BRCA1_87_FOR | CCACAAATACAAATTATGACCAAGATTTTTGGC | >520999_BRCA1_87_REV | ACTCTGAGGACAAAGCAGCGG | ||
| >520999_BRCA1_88_FOR | CTTACCCAATTCAATGTAGACAGACGTC | >520999_BRCA1_88_REV | TGGCCAACAATTGCTTGACTGTTC | ||
| >520999_BRCA1_89_FOR | CAAGGTTAGAGAGTTGGACACTGAG | >520999_BRCA1_89_REV | GGTCAATTTATTTTGTCCATGGTGTCAAG | ||
| >520999_BRCA1_90_FOR | CTGGGCCACAGAGCAAGACTCCATCTC | >520999_BRCA1_90_REV | GATGAAGTTTCTATCATCCAAAGTATGGGCTAC | ||
| >520999_BRCA1_91_FOR | GGTTCACTCTGTAGAAGTCTTTTGGC | >520999_BRCA1_91_REV | CATTTTTCTCTAACTGCAAACATAATGTTTTCCCTTG | ||
| >520999_BRCA1_92_FOR | ACCACGTCATAGAAAGTAATTGTGCAAAC | >520999_BRCA1_92_REV | CGAGATTTAGTCAACTTGTTGAAGAGCTA | ||
| >520999_BRCA1_93_FOR | GGGATATTCAACACTTACACTCCAAACC | >520999_BRCA1_93_REV | AGGTTGATAATCACTTGCTGAGTGTG | ||
| >520999_BRCA1_94_FOR | CTTTTTCCTACTGTGGTTGCTTCCAACC | >520999_BRCA1_94_REV | GATTTTGCATGCTGAAACTTCTCAACCAG | ||
| >520999_BRCA1_95_FOR | CATTCTTACATAAAGGACACTGTGAAGGC | >520999_BRCA1_95_REV | GATTATCTTTTCATGGCTATTTGCCTTTTGAGTATTC | ||
| >520999_BRCA1_96_FOR | AGCTTCTATAAAGTTAGGTGTTTCCTGGG | >520999_BRCA1_96_REV | TACCCTGCTAGTCTGGAGTTGA | ||
| >520999_BRCA1_97_FOR | CATTCAAACTTACTTGCAAAATATGTGGTCAC | >520999_BRCA1_97_REV | ACAGCTCAAAGTTGAACTTATTCACTAAG | ||
| >520999_BRCA1_98_FOR | CATAGGAATCCCAAATTAATACACTCTTGTGC | >520999_BRCA1_98_REV | CTAATGTGTTAAAGTTCATTGGAACAGAAAGAAATGG | ||
| >520999_BRCA1_99_FOR | GACATTTTGTACTTCTTCAACGCGAAG | >520999_BRCA1_99_REV | GTTCTTTGGTTTGTATTATTCTAAAACCTTCCAAATC | ||
| >520999_CYP2D6_01_FOR | 45 | GTACATTAGAGCCTCTGGCTAGGGAGCAG | >520999_CYP2D6_01_REV | 46 | ACTTCAGCTTCTCGGTGCCCACTGG |
| >520999_CYP2D6_02_FOR | 47 | GCTCACCAGGAAAGCAAAGACACCATGG | >520999_CYP2D6_02_REV | 48 | GTATCACCCAGGAGCCAGGCTCACTGAC |
| >520999_CYP2D6_03_FOR | 49 | GCAAGACTCCACGGAAGGGGACAGG | >520999_CYP2D6_03_REV | 50 | GCTGAAGGATGAGGCCGTCTGGGAG |
| >520999_CYP2D6_04_FOR | 51 | CATCCAGGAAGTGTTCGGGGTGGAAG | >520999_CYP2D6_04_REV | 52 | CCAGCATCCTAGAGTCCAGTCCCCAC |
| >520999_CYP2D6_05_FOR | 53 | CTATCACCAGGTGCTGGTGCTGAGCTG | >520999_CYP2D6_05_REV | 54 | ATGCCCTACACCACTGCCGTGATTCATG |
| >520999_CYP2D6_06_FOR | 55 | TCATATGGGTCACACCCAGGGGGACG | >520999_CYP2D6_06_REV | 56 | GTGAGGACGCATGTCTGTCCAGGCC |
| >520999_CYP2D6_07_FOR | 57 | ACCTCATGAATCACGGCAGTGGTGTAGG | >520999_CYP2D6_07_REV | 58 | GCAAGAAGGAGTGTCAGGGCCGGAC |
| >520999_CYP2D6_08_FOR | 59 | CACGGGTGTCCCAGCAAAGTTCATGG | >520999_CYP2D6_08_REV | 60 | CTGACAGGATGGTGACCACCTCGACC |
| >520999_CYP2D6_09_FOR | 61 | GATGAGCTCACGCTGCACATCCGGATG | >520999_CYP2D6_09_REV | 62 | CTCAGACATGCTCAGGCCAAGGGGAAC |
| >520999_CYP2D6_10_FOR | 63 | CCATCCCGGCAGAGAACAGGTCAGC | >520999_CYP2D6_10_REV | 64 | CTACCCCGTTCTGTCCCGAGTATGCTC |
| >520999_CYP2D6_11_FOR | 65 | GACTACCCTCATTCCTCCTGGGACGCTC | >520999_CYP2D6_11_REV | 66 | GATGAGCTGCTAACTGAGCACAGGATGACC |
| >520999_CYP2D6_12_FOR | 67 | CAGCCACTCTCACCTTCTCCATCTCTGC | >520999_CYP2D6_12_REV | 68 | GAATGCTGTCCCCGTCCTCCTGCATATC |
| >520999_CYP2D6_13_FOR | 69 | GAAAGCCTTTTGGAAGCGTAGGACCTTGC | >520999_CYP2D6_13_REV | 70 | GAGATGGCTGGGGCCTGAGACTTGTC |
| >520999_CYP2D6_14_FOR | 71 | CTGCAGAGACTCCTCGGTCTCTCGC | >520999_CYP2D6_14_REV | 72 | GAAGACCCTTTCGCCCCAACGGTCTC |
| >520999_CYP2D6_15_FOR | 73 | GAAGCGAGGGTCGTCGTACTCGAAGC | >520999_CYP2D6_15_REV | 74 | GGAAGACAGAGGACGGGGAAGGCGAC |
| >520999_CYP2D6_16_FOR | 75 | GAGAGATGAGAGTAAGGGGTCGCCTTCCC | >520999_CYP2D6_16_REV | 76 | GACTGGGCAAGAAGTCGCTGGAGCAG |
| >520999_CYP2D6_17_FOR | 77 | CTGACCATCACCCACCGGAGTGGTTG | >520999_CYP2D6_17_REV | 78 | CACACACACAGGGGTGTTCCTGGCG |
| >520999_CYP2D6_18_FOR | 79 | CACAGACCCAAGTTGCGCAAGGTGG | >520999_CYP2D6_18_REV | 80 | GTGGGTGGTGGATGGTGGGGCTAATG |
| >520999_CYP2D6_19_FOR | 81 | CCGGGTCCCACGGAAATCTGTCTCTG | >520999_CYP2D6_19_REV | 82 | GACAGTGGTCGTGCTCAATGGGCTG |
| >520999_CYP2D6_20_FOR | 83 | CAACAGCTGCTTGCCTTGGGAACGC | >520999_CYP2D6_20_REV | 84 | CTTCGGGGACGTGTTCAGCCTGCAG |
| >520999_CYP2D6_21_FOR | 85 | CACATTGAGCACGACCACCGGCGTC | >520999_CYP2D6_21_REV | 86 | GGTTCAAATAGGACTAGGACCTGTAGTCTGGGG |
| >520999_CYP2D6_22_FOR | 87 | GCTTCTGGTCCAGCCTGTGGTTTCAC | >520999_CYP2D6_22_REV | 88 | GCAACCTGCTGCATGTGGACTTCCAG |
| >520999_CYP2D6_23_FOR | 89 | GACCTCCTCCCTCACCTGGTCGAAGC | >520999_CYP2D6_23_REV | 90 | GCCATCTTCCTGCTCCTGGTGGACC |
| >520999_CYP2D6_24_FOR | 91 | GATAGAGTGCAGCCCAGCGTTGGCG | >520999_CYP2D6_24_REV | 92 | CCAGGTGTGTCCAGAGGAGCCCATTTG |
| >520999_BRAF_01_FOR | 93 | CTTTCTAGTAACTCAGCAGCATCTCAGGG | >520999_BRAF_01_REV | 94 | GGTGATTTTGGTCTAGCTACAGTGAAATCTCG |
| >520999_BRAF_02_FOR | 95 | GGATCCAGACAACTGTTCAAACTGATGG | >520999_BRAF_02_REV | 96 | GCCTAAACTCTTCATAATGCTTGCTCTGATAGG |
본 발명에 있어서, 상기 앰플리콘은 변이가 없는 암 유전자 및 항암제 대사 관련 유전자의 야생형을 상기 프라이머로 증폭하여 얻어진 증폭 산물일 수 있으며, 바람직하게는 하기 표 3에 기재된 서열로부터 선택되는 것일 수 있으며, 더욱 바람직하기로는 서열번호 97 내지 128의 서열로부터 선택되는 것일 수 있다.
| 앰플리콘명 | 서열번호 | 앰플리콘 서열 | |
| >520999_AKT1_01 | CCAGCACCCCGCCATCCCCGTGTCCCTCCTAAGCGCTGGGGCTGCCCAAGTGCCTGGCCTGGCCGCCACAGCCCACGTACCGCTCCTCAGGAGTCTCCACATGGAAGGTGC | ||
| >520999_AKT1_02 | ATGGAAGGTGCGTTCGATGACAGTGGTCCACTGCAGGCAGCGGATGATGAAGGTGTTGGGCCGGGGC | ||
| >520999_AKT1_03 | GGCCGGGGCCGCTCCGTCTTCATCAGCTGGCACTCTGCGGGCAGGCAGAGCCTCTGTCTGCGTGCATCCCCCTGCCCCTCCCAGGGCCCTCACCACAGCCCCCGCTG | ||
| >520999_AKT1_04 | CCCGAGAGGCCAAGGGGATACTTACGCGCCACAGAGAAGTTGTTGAGGGGAGCCTCACGTTGGTCCACATCCTGCGGCCGCTCCTT | ||
| >520999_AKT1_05 | CGTTGGTCCACATCCTGCGGCCGCTCCTTGTAGCCAATGAAGGTGCCATCATTCTTGAGGAGGAAGTAGCGTGGCCGCCAG | ||
| >520999_AKT1_06 | CCGCCAGGTCTTGATGTACTCCCCTACAGACGTGCGGGTGGTGAGAGCCACGCACACTCTACC | ||
| >520999_BRAF_01 | CCAAAAATTTAATCAGTGGAAAAATAGCCTCAATTCTTACCATCCACAAAATGGATCCAGACAACTGTTCAAACTGATGGGACCCACTCCAT | ||
| >520999_BRAF_02 | GACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCACCTATTTTTACTGTGAGGTCTTCATGAAGAAATATATCTGAGGTGTAGTAAGTAAAGGAAAACAGTAGATCTCATTTT | ||
| >520999_BRCA1_01 | AAAATCTTTAAGGGACCCTTGCATAGCCAGAAGTCCTTTTCAGGCTGATGTACATAAAATATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCAGGGCCT | ||
| >520999_BRCA1_02 | AGCTCCCAGGGCCTGGAAAGGCCACTTTGTAAGCTCATTCTTGGGGTCCTGTGGCTCTGTACCTGTGGCTGGCTGCA | ||
| >520999_BRCA1_03 | GCTGGCTGCAGTCAGTAGTGGCTGTGGGGGATCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACACTGTCCAACACCCACTCTCGGG | ||
| >520999_BRCA1_04 | ACACTGTCCAACACCCACTCTCGGGTCACCACAGGTGCCTCACACATCTGCCCAATTGCTGGAGACAGAGAACACAAGCAGAGATTAGTGTCAATTCATT | ||
| >520999_BRCA1_05 | AGGTAATGAGTGATAAACCAAACCCATGCAAAAGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCATTGTCCTCTGTCCAGGCA | ||
| >520999_BRCA1_06 | CATGGAAGCCATTGTCCTCTGTCCAGGCATCTGGCTGCACAACCACAATTGGGTGGACACCCTGGATCCCCAGGAAGGAAAGAGCATTCAAAGTGTCAAA | ||
| >520999_BRCA1_07 | GGGACTGACAGGTGCCAGTCTTGCTCACAGGAGAGAATATTGTGTCCTCCCTCTCTGACAGGGCACCCAATACTTACTGTGCCAAGGGTGAATGATGAA | ||
| >520999_BRCA1_08 | CCAAGGGTGAATGATGAAAGCTCCTTCACCACAGAAGCACCACACAGCTGTACCATCCATTCCAGTTGATCTAAAATGGACATTTAGATGTAAAATCACT | ||
| >520999_BRCA1_09 | CGTGGGATCTTGCTTATAATACTCCACTATGTAAGACAAAGGCTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTTGGTGAAGG | ||
| >520999_BRCA1_10 | CCAGGCTCTTACCTGTGGGCATGTTGGTGAAGGGCCCATAGCAACAGATTTCTAGCCCCCTGAAGATCTGGAAGAAGAGAGGAAGAGAGAGGGACA | ||
| >520999_BRCA1_11 | GCAAAGGGGAGTGGAATACAGAGTGGTGGGGTGAGATTTTTGTCAACTTGAGGGAGGGAGCTTTACCTTTCTGTCCTGGGATTCTCTTGCTCGCTTTGGA | ||
| >520999_BRCA1_12 | CTGTCCTGGGATTCTCTTGCTCGCTTTGGACCTTGGTGGTTTCTTCCATTGACCACATCTCCTCTGACTTCAAAATCATGCTGAAAGAAACCAAACACAA | ||
| >520999_BRCA1_13 | AAAATCATGCTGAAAGAAACCAAACACAACCCATCAGGATAAGAGAAAGAGAAGCTTCCTTCAATGGAAGTGGAGCAGACACGTCATAT | ||
| >520999_BRCA1_14 | GAAGCAAATACATTTTTAACTATATGACTGAATGAATATCTCTGGTTAGTTTGTAACATCAAGTACTTACCTCATTCAGCATTTTTCTTTCTTTAATAGA | ||
| >520999_BRCA1_16 | TTAAAGGGAGGAGGGGAGAAATAGTATTATACTTACAGAAATAGCTAACTACCCATTTTCCTCC | ||
| >520999_BRCA1_18 | TTCTTGGTATACCTGTTTTCATAACAACATGAGTAGTCTCTTCAGTAATTAGATTAGTTAAAGTGATGTGGTGTTTTCT | ||
| >520999_BRCA1_20 | ATAAAACTCTTTCCAGAATGTTGTTAAGTCTTAGTCATTAGGGAGATACATATGGATACACTCACAAATTCTTCTGGGGTCAGGCCAGACACCACCATGG | ||
| >520999_BRCA1_22 | CATTGCATTATACCCAGCAGTATCAGTAGTATGAGCAGCAGCTGGACTCTGGGCAGATTCTGCAACTTTCAATTGGGGAACTTTCAATGCAGAGGTTGAA | ||
| >520999_BRCA1_24 | GATCAGATTCAGGGTCATCAGAGAAGAGGCTGATTCCAGATTCCAGGTAAGGGGTTCCCTCTGAAAGGAATGGGAGAAGTTTAATTTACACAACGATGAA | ||
| >520999_BRCA1_26 | AAGTAAGATGTTTCCGTCAAATCGTGTGGCCCAGACTCTTCCAGCTGTTGCTCCTCCACATCAACAACCTTAATGAGCTCCTCTTGAGATGGGTAGTTTC | ||
| >520999_BRCA1_28 | ATTTAGAAGGGGATGACCTAGAAAGATAAATGGAAGGAGAAAACCATCGCCACCAATTGTGAAAGGACAAATCATACTTGCTG | ||
| >520999_BRCA1_30 | AGCAGAAAGGCCTTCTGGATTCTGGCTTATAGGGTATTCACTACTTTTCTGTGAAGTTAATACTGCTTTAAATGGAATGAGAAAACAAATCTACTTTA | ||
| >520999_BRCA1_32 | TATTAAAAAGTCATTCCTCCTTTTGGCCAGAACCACCATCTTTCAGTAATTTGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCA | ||
| >520999_BRCA1_34 | AGATAAAGGGGAAGGAAAGAATTTTGCTTAAGATATCAGTGTTTGGCCAACAATACACACCTTTTTCTGATGTGCTTTGTTCTGGATTTCGCAGGTCCTCAAGGGCAGAA | ||
| >520999_BRCA1_36 | CATGCTGTTCTAACACAGCTTCTAGTTCAGCCATTTCCTGCTGGAGCTTTATCAGGTTATGTTGCATGGTATCCCTCTGCTTCAAAAACGATAAATGGCA | ||
| >520999_BRCA1_38 | GGTTAAAATGTCACTCTGAGAGGATAGCCCTGAGCAGTCTTCAGAGACGCTTGTTTCACTCTCACACCCAGATGCTGCTTCACCTTAAATAACAAAAA | ||
| >520999_BRCA1_40 | ATAAACATTTAGCTCACTTCTATAAATAGACTGGGGCAAACACAAAAACCTGGTTCCAATACCTAAGTTTGAATCCATGCTTTGCTCTTCTTGATTATTT | ||
| >520999_BRCA1_42 | AACTCCCTGGCTTTCAGACTGATGCCTCATTTGTTTGGAAGAACCAATCAAGAAAGGATCCTGGGTGTTTGTATTTGCAGTCAAGTCTTCCAATTCACTG | ||
| >520999_BRCA1_44 | CTTTGCCAATATTACCTGGTTACTGCAGTCATTTAAGCTATTCTTCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCAACGGTGCTATGCCT | ||
| >520999_BRCA1_46 | CTCTTCATCCTCACTAGATAAGTTCTCTTCTGAGGACTCTAATTTCTTGGCCCCTCTTCGGTAACCCTGAGCCAAATGTGTATGGGTGAAAGGGCTAGG | ||
| >520999_BRCA1_48 | AAACTAGTATCTTCCTTTATTTCACCATCATCTAACAGGTCATCAGGTGTCTCAGAACAAACCTGAGATGCATGACTACTTCCCATAGGCTGTTCTAAGTTATCTGAAATCAGATA | ||
| >520999_BRCA1_50 | AAGACTTTGTTTATAGACCTCAGGTTGCAAAACCCCTAATCTAAGCATAGCATTCAATTTTGGCCCTCTGTTTCTACCTAGTTCTGCTTGAATGTTTTCA | ||
| >520999_BRCA1_52 | AACCTACTTCATTAATATTGCTTGAGCTGGCTTCTTTAAAAACATTTTCTCTAATGTTATTACGGCTAATTGTGCTCACTGTACTTGGAATGTTCTCATT | ||
| >520999_BRCA1_54 | ATTTTTCTTACATTTAGTTTTAACAAATGACTTGATGGGAAAAAGTGGTGGTATACGATATGGGTTTTGTAAAAGTCCATGTTTATTTGGAGTAATGAGT | ||
| >520999_BRCA1_56 | TCCTTTGATACTACATTTGGCATTATCAACTGGCTTATCTTTCTGACCAACCACAGGAAAGCCTGCAGTGATATTAACTGTCTGTACAGGCTTGATATTA | ||
| >520999_BRCA1_58 | TTTGTTTCTTTAAGGACCCAGAGTGGGCAGAGAATGTTGCACATTCCTCTTCTGCATTTCCTGGATTTGAAAACGGAGCAAATGACTGGCGCTTTGAAA | ||
| >520999_BRCA1_60 | ACTGTGGTTAACTTCATGTCCCAATGGATACTTAAAGCCTTCTGTGTCATTTCTATTATCTTTGGAACAACCATGAATTAGTCCCTTGGGGTTTTCAAA | ||
| >520999_BRCA1_62 | AACGAGATACTTTCCTGAGTGCCATAATCAGTACCAGGTACCAATGAAATACTGCTACTCTCTACAGATCTTTCAGTTTGCAAAACCCTTTCTCCACTTAA | ||
| >520999_BRCA1_64 | TCTTTTTCTTCTCTTGGAAGGCTAGGATTGACAAATTCTTTAAGTTCACTGGTATTTGAACACTTAGTAAAAGAACCAGGTGCATTTGTTAACTTCAGCTCTGGGAAAGTATCGCTGTCAT | ||
| >520999_BRCA1_66 | TCTTTACCTTCCATGAGTTGTAGGTTTCTGCTGTGCCTGACTGGCATTTGGTTGTACTTTTTTTTCTTTATCTCTTCACTGCTAGAACAACTATCAATTT | ||
| >520999_BRCA1_68 | TGGTAGAAGACTTCCTCCTCAGCCTATTCTTTTTAGGTGCTTTTGAATTGTGGATATTTAATTCGAGTTCCATATTGCTTATACTGCTGCTTATA | ||
| >520999_BRCA1_70 | TTTTGTTTTATTCTCATGACCACTATTAGTAATATTCATCACTTGACCATTCTGCTCCGTTTGGTTAGTTCCCTGATTTATCATTTCAGGAGTCTTTTGA | ||
| >520999_BRCA1_72 | TGTAGGTCTCCTTTTACGCTTTAATTTATTTGTGAGGGGACGCTCTTGTATTATCTGTGGCTCAGTAACAAATGCTCCTATAATTAGATTTTCAGTTACA | ||
| >520999_BRCA1_74 | TTCAATATTACTCTCTACTGATTTGGAGTGAACTCTTTCACTTTTACATATTAAAGCCTCATGAGGATCACTGGCCAGTAAGTCTATTTTCTCTGAAGAA | ||
| >520999_BRCA1_76 | ATTTGATTCAGACTCCCCATCATGTGAGTCATCAGAACCTAACAGTTCATCACTTCTGGAAAACCACTCATTAACTTTCTGAATGCTGCTATTTAGTGTTAT | ||
| >520999_BRCA1_78 | GGATCAGCATTCAGATCTACCTTTTTTTCTGTGCTGGGAGTCCGCCTATCATTACATGTTTCCTTACTTCCAGCCCATCT | ||
| >520999_BRCA1_80 | GCTGTTTGCTTTTATTACAGAATTCAGCCTTTTCTACATTCATTCTGTCTTTAGTGAGTAATAAACTGCTGTTCTCATGCTGTAATGAGCTGGCATGAGT | ||
| >520999_BRCA1_82 | AATCATTATTACTGGGTTGATGATGTTCAGTATTTGTTACATCCGTCTCAGAAAATTCACAAGCAGCTGAAAATATACAAAAATAACAAGGTACTCAAAAACTGAATTGTCATTAAAAAAAT | ||
| >520999_BRCA1_84 | ATAATCTAATTACAGTACTGTATCTACCCACTCTCTTTTCAGTGCCTGTTAAGTTGGCAAACTTTGCCATTACCCTTTTTTGCAGAATCCAAACTGATTT | ||
| >520999_BRCA1_86 | TTTGACTCACCTGCAATAAGTTGCCTTATTAACGGTATCTTCAGAAGAATCAGATCCTAAAAAATTTCC | ||
| >520999_BRCA1_88 | TTTTGAGGTTGTATCCGCTGCTTTGTCCTCAGAGTTCTCACAGTTCCAAGGTTAGAGAGTTGGACACTGAGACTGGTTTCCTGCTAAACAGTATGGTAAA | ||
| >520999_BRCA1_90 | AAAAAAAAAAAAGAAAAAAAAAAGAAAAGAAGAAGAAGAAGAAGAAGAAAACAAATGGTTTTACCAAGGAAGGATTTTCGGGTTCACTCTGTAGAAGTCTTTTGGCACGGTTTCT | ||
| >520999_BRCA1_92 | TTCCTGAGTTTTCATGGACAGCACTTGAGTGTCATTCTTGGGATATTCAACACTTACACTCCAAACCTGTGTCAAGCTGAAAAGCACAAATGATTTTCAA | ||
| >520999_BRCA1_94 | TAGCATCATTACCAAATTATATACCTTTTGGTTATATCATTCTTACATAAAGGACACTGTGAAGGCCCTTTCTT | ||
| >520999_BRCA1_96 | TTATGAAGGACAAAAACAAAAGCTAATAATGGAGCCACATAACACATTCAAACTTACTTGCAAAATATGTGGTCACACTTTGTGGAGACAGGTTCCTTGA | ||
| >520999_BRCA1_97 | ACTTTGTGGAGACAGGTTCCTTGATCAACTCCAGACTAGCAGGGTAGGGGGGGAGAAAAAGAAAATAAATGAGGCTCAATAATTTATTTAAAAATAAAGCTATT | ||
| >520999_BRCA1_98 | TGACTTACCAGATGGGACACTCTAAGATTTTCTGCATAGCATTAATGACATTTTGTACTTCTTCAACGCGAAGAGCAGATAAAT | ||
| >520999_BRCA1_99 | AGCAGATAAATCCATTTCTTTCTGTTCCAATGAACTTTAACACATTAGAAAAACATATATATATATCTTTTTAAAAGGTTTATAAAATGACAACTTCATTTTATCATTTTAAAATAAAGTAAATTTAA | ||
| >520999_BRCA2_01 | TCAGTCACATAATAAGGAATGCATCCCTGTGTAAGTGCATTTTGGTCTTCTGTTTTGCAGACTTATTTACCAAGCATTGGAGGAATATCGTAGGTAAAAA | ||
| >520999_BRCA2_02 | TAGGTAAAAATGCCTATTGGATCCAAAGAGAGGCCAACATTTTTTGAAATTTTTAAGACACGCTGCAACAAAGCAGGTATTGACAAATTTTATATAACTTTATAAATT | ||
| >520999_BRCA2_03 | ACAAATTTGTCTGTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTTAAATAGATTTAGGACCAATAAGTCTTAATTGGTTTGAAGAACTTTCTTCAGAA | ||
| >520999_BRCA2_04 | TTTCTTCAGAAGCTCCACCCTATAATTCTGAACCTGCAGAAGAATCTGAACATAAAAACAACAATTACGAACCAAACCTATTTAAAACTCCACAAAGGAA | ||
| >520999_BRCA2_05 | CACAAAGGAAACCATCTTATAATCAGCTGGCTTCAACTCCAATAATATTCAAAGAGCAAGGGCTGACTCTGCCGCTGTACCAATCTCCTGTAAAAGAATT | ||
| >520999_BRCA2_06 | CGCTGTACCAATCTCCTGTAAAAGAATTAGATAAATTCAAATTAGACTTAGGTAAGTAATGCAATATGGTAGACTGGGGAGAACTACAAACTAGGAATTTA | ||
| >520999_BRCA2_07 | ATTATTGTACTGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAGTGAAAACTAAAATGGATCAAGCAGATGATGTTTCCTGTCCACTTCTAAATTCTT | ||
| >520999_BRCA2_08 | TTCTAAATTCTTGTCTTAGTGAAAGGTATGATGAAGCTATTATATTAAAATATTTAAATGAAACATTTTCCTACATATATTTGTTCTATAAAGATGAATCTGATTTTTATGCTAATATTTT | ||
| >520999_BRCA2_09 | TTTTATTTTAGTCCTGTTGTTCTACAATGTACACATGTAACACCACAAAGAGATAAGTCAGGTATGATTAAAAA | ||
| >520999_BRCA2_10 | ATGATTAAAAACAATGCTTTTTATTCTTAGAATACTAGAAATGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTGGTATGTGGGAGTTTGTTTCAT | ||
| >520999_BRCA2_100 | TTTTGTCAAATTCAAGAATTGGAAAAAGAAGAGGAGAGCCCCTTATCTTAGTGGGTAAGTGTTCATTTTTA | ||
| >520999_BRCA2_101 | TTAAAAACATATATGAAATATTTCTTTTTAGGAGAACCCTCAATCAAAAGAAACTTATTAAATGAATTTGACAGGATAATAGAAAATCAAGAAAAATC | ||
| >520999_BRCA2_102 | ATAATAGAAAATCAAGAAAAATCCTTAAAGGCTTCAAAAAGCACTCCAGATGGTAAAATTAGCTTTTTATTTATAT | ||
| >520999_BRCA2_103 | TGGATATTCTCTTAGATTTTAACTAATATGTAATATAAAATAATTGTTTCCTAGGCACAATAAAAGATCGAAGATTGTTTAT | ||
| >520999_BRCA2_104 | AATAAAAGATCGAAGATTGTTTATGCATCATGTTTCTTTAGAGCCGATTACCTGTGTACCCTTTCGGTAAGACATGTTTAAATTTTT | ||
| >520999_BRCA2_105 | AGGCATATTCCTAAATATTTATATGTGTACTAGTCAATAAACTTATATATTTTCTCCCCATTGCAGCACAACTAAGGAACGTCAAGAGATACAGAATCCAAATTTTAC | ||
| >520999_BRCA2_106 | AAATTTTACCGCACCTGGTCAAGAATTTCTGTCTAAATCTCATTTGTATGAACATCTGACTTTGGAAAAATCTTCAAGCAATTTA | ||
| >520999_BRCA2_107 | AAAAATCTTCAAGCAATTTAGCAGTTTCAGGACATCCATTTTATCAAGTTTCTGCTACAAGAAATGAAAAAATGAGACACTTGATTA | ||
| >520999_BRCA2_108 | TTGATTACTACAGGCAGACCAACCAAAGTCTTTGTTCCACCTTTTAAAACTAAATCACATTTTCACAGAGTTGAACAGTGTGTTAGGAATATTAACTTGGAGGAAAACAGACAAAA | ||
| >520999_BRCA2_109 | TAACTTGGAGGAAAACAGACAAAAGCAAAACATTGATGGACATGGCTCTGATGATAGTAAAAATAAGATTAATGACAATGAGATTCATCAGTTTAACAAAAA | ||
| >520999_BRCA2_11 | TGTTTCATACACCAAAGTTTGTGAAGGTAAATATTCTACCTGGTTTATTTTTATGACTTAGTAATTGAGAATTTGACAATAGCGTTATACCTTTG | ||
| >520999_BRCA2_110 | ATTCATCAGTTTAACAAAAACAACTCCAATCAAGCAGTAGCTGTAACTTTCACAAAGTGTGAAGAAGAACCTTTAGGTATTGTATGACAATTTGTGTGATGAATTTTT | ||
| >520999_BRCA2_111 | TATTTATTCTTTGATAGATTTAATTACAAGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACAAAGGCAACG | ||
| >520999_BRCA2_112 | ATATACAGGATATGCGAATTAAGAAGAAACAAAGGCAACGCGTCTTTCCACAGCCAGGCAGTCTGTATCTTGC | ||
| >520999_BRCA2_113 | ACAGCCAGGCAGTCTGTATCTTGCAAAAACATCCACTCTGCCTCGAATCTCTCTGAAAGCAGCAGTAGGAGGCCAAGTTCCCTCTGCGTGTTCTCATAAA | ||
| >520999_BRCA2_114 | CTCATAAACAGGTATGTGTTTGTCTACAATACTGATGGCTTTTATGACAGAGTGTAATTTTATTTCATTAACTAGTATCT | ||
| >520999_BRCA2_115 | GATACATGTTTACTTTAAATTGTTTTTCTTTTTTGTGTGTGTTTATTTTGTGTAGCTGTATACGTATGGCGTTTCTAAACA | ||
| >520999_BRCA2_116 | TTTCTAAACATTGCATAAAAATTAACAGCAAAAATGCAGAGTCTTTTCAGTTTCACACTGAAGATTATTTTGGTAAGGAAAGTTTATGGACTGGAAAAGG | ||
| >520999_BRCA2_117 | AAAAGGAATACAGTTGGCTGATGGTGGATGGCTCATACCCTCCAATGATGGAAAGGCTGGAAAAGAAGAATTTTATAGGTACTCTATGCAAAAAGATTGT | ||
| >520999_BRCA2_118 | TTTTTATGATAATATTCTACTTTTATTTGTTCAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGCTTATTTCTAGAATTTGGGTTTATAAT | ||
| >520999_BRCA2_119 | AGGTGTGGATCCAAAGCTTATTTCTAGAATTTGGGTTTATAATCACTATAGATGGATCATATGGAAACTGGCAGCTATGGAATGTGCCTTTCCTAA | ||
| >520999_BRCA2_12 | AAAAAATAATATCCTTAATGATCAGGGCATTTCTATAAAAAATAAACTATTTTCTTTCCTCCCAGGGTCGTCAGACACCAAAACATATTTCTGAAA | ||
| >520999_BRCA2_120 | TATGGAATGTGCCTTTCCTAAGGAATTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATACAGGCA | ||
| >520999_BRCA2_121 | AAGGGTGCTTCTTCAACTAAAATACAGGCAAGTTTAAAGCATTACATTACGTAATCATATACGGCAGTATGGTTAAGGTTTCTGTGTAGTCTGTGACTT | ||
| >520999_BRCA2_122 | TAAACAGTGGAATTCTAGAGTCACACTTCCTAAAATATGCATTTTTGTTTTCACTTTTAGATATGATACGGAAATTGATA | ||
| >520999_BRCA2_123 | AAATTGATAGAAGCAGAAGATCGGCTATAAAAAAGATAATGGAAAGGGATGACACAGCTGCAAAAACACTTGTTCTCTGTGTTTCTGACATAATTTCATT | ||
| >520999_BRCA2_124 | TTGTTCTCTGTGTTTCTGACATAATTTCATTGAGCGCAAATATATCTGAAACTTCTAGCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATT | ||
| >520999_BRCA2_125 | AAAAGTGGCCATTATTGAACTTACAGATGGGTGGTATGCTGTTAAGGCCCAGTTAGATCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTT | ||
| >520999_BRCA2_126 | TAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGAAGATTATTCTTCATGGAGCAGAACTGGTGGGCTCTCCTGATGCCTGTACACCTCTTGAA | ||
| >520999_BRCA2_127 | ACCTCTTGAAGCCCCAGAATCTCTTATGTTAAAGGTAAATTAATTTGCACTCTTGGTAAAAATCAGTCATTGATTCAGTTAAATTCTAGAAGTTTTACAT | ||
| >520999_BRCA2_128 | TAATAGAATTGAATACATATTTAACTACTAAATCAATATATTTATTAATTTGTCCAGATTTCTGCTAACAGTACTCGGCCTG | ||
| >520999_BRCA2_129 | GGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCTGACCCTAGACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTTGGTTGTGTTGATGTAATTATTCAAA | ||
| >520999_BRCA2_13 | TCAGACACCAAAACATATTTCTGAAAGTCTAGGAGCTGAGGTGGATCCTGATATGTCTTGGTCAAGTTCTTTAGCTACACCACCCA | ||
| >520999_BRCA2_130 | GAAATGTTGGTTGTGTTGATGTAATTATTCAAAGAGCATACCCTATACAGGTATGATGTATTCTTGAAACTTACCATATATTTCTTTCTTTTGATACAATTAATTTGTTT | ||
| >520999_BRCA2_131 | ATTATTACAGTGGATGGAGAAGACATCATCTGGATTATACATATTTCGCAATGAAAGAGAGGAAGAAAAGGAAGCAGCAAAATATGTGGAGGCCCAACAAAAGAGACTA | ||
| >520999_BRCA2_132 | AAAATATGTGGAGGCCCAACAAAAGAGACTAGAAGCCTTATTCACTAAAATTCAGGAGGAATTTGAAGAACATGAAGGTAAAATTAGTTATAT | ||
| >520999_BRCA2_133 | TTCTTTAGTTTTAGTTGCTTTTGAATTTACAGTTTAGTGAATTAATAATCCTTTTGTTTTCTTAGAAAACACAACAAAACCATATTTACCATCACGTGCA | ||
| >520999_BRCA2_134 | ACGTGCACTAACAAGACAGCAAGTTCGTGCTTTGCAAGATGGTGCAGAGCTTTATGAAGCAGTGAAGAATGCAGCAGACCCAGCTTACCTTGAGGTGAGA | ||
| >520999_BRCA2_135 | CAGAGCTTTATGAAGCAGTGAAGAATGCAGCAGACCCAGCTTACCTTGAGGTGAGAGAGTAAGAGGACATATAATGAGGCTTGATGATTATTCAAGGTGA | ||
| >520999_BRCA2_136 | TTTTTATTCCAATATCTTAAATGGTCACAGGGTTATTTCAGTGAAGAGCAGTTAAGAGCCTTGAATAATCACA | ||
| >520999_BRCA2_137 | AGTTAAGAGCCTTGAATAATCACAGGCAAATGTTGAATGATAAGAAACAAGCTCAGATCCAGTTGGAAATTAGGAAGGCCATGGAATCTGCTGAACAAAA | ||
| >520999_BRCA2_138 | GCCATGGAATCTGCTGAACAAAAGGAACAAGGTTTATCAAGGGATGTCACAACCGTGTGGAAGTTGCGTATTGTAAGCTATTCAAAAAAAGAAAAAGATT | ||
| >520999_BRCA2_139 | GTGGAAGTTGCGTATTGTAAGCTATTCAAAAAAAGAAAAAGATTCAGGTAAGTATGTAAATGCTTTGTTTTTATCAGTTTTATTAACTTAAAA | ||
| >520999_BRCA2_14 | CTTGGTCAAGTTCTTTAGCTACACCACCCACCCTTAGTTCTACTGTGCTCATAGGTAATAATAGCAAATGTGTATTTACAAGAAA | ||
| >520999_BRCA2_140 | TTAAATGATAATCACTTCTTCCATTGCATCTTTCTCATCTTTCTCCAAACAGTTATACTGAGTATTTGGCGTCCATCATCAGATTTATATTCTCTGTTAA | ||
| >520999_BRCA2_141 | TTAACAGAAGGAAAGAGATACAGAATTTATCATCTTGCAACTTCAAAATCTAAAAGTAAATCTGAAAGAGCTAACATACAGTTAGCAGCGACAAAAAAAA | ||
| >520999_BRCA2_142 | AGCGACAAAAAAAACTCAGTATCAACAACTACCGGTACAAACCTTTCATTGTAATTTTTCAGTTTTGATAAGTGCTTGTTAGTTTATGGAATCTCCATAT | ||
| >520999_BRCA2_143 | AATCTCCATATGTTGAATTTTTGTTTTGTTTTCTGTAGGTTTCAGATGAAATTTTATTTCAGATTTACCAGCCACGGGAGCCCCTTCACTTCAGCAAATT | ||
| >520999_BRCA2_144 | GAGCCCCTTCACTTCAGCAAATTTTTAGATCCAGACTTTCAGCCATCTTGTTCTGAGGTGGACCTAATAGGATTTGTCGTTT | ||
| >520999_BRCA2_145 | TAGGATTTGTCGTTTCTGTTGTGAAAAAAACAGGTAATGCACAATATAGTTAATTTTTTTTATTGATTCTTTTAAAAAACATTGTCTTTTAAAATCTCTTA | ||
| >520999_BRCA2_146 | TTAAAATTCATCTAACACATCTATAATAACATTCTTTTCTTTTTTTTCCATTCTAGGACTTGCCCCTTTCGTCTATTTGTCAGACGAATGTTACAATTT | ||
| >520999_BRCA2_147 | TATTTGTCAGACGAATGTTACAATTTACTGGCAATAAAGTTTTGGATAGACCTTAATGAGGACATTATTAAGCCTCATATGTTAATTGCTGCAAGCAACC | ||
| >520999_BRCA2_148 | TATGTTAATTGCTGCAAGCAACCTCCAGTGGCGACCAGAATCCAAATCAGGCCTTCTTACTTTATTTGCTGGAGATTTTTCTGTGTTTTCTGCTAGTCCA | ||
| >520999_BRCA2_149 | AGATTTTTCTGTGTTTTCTGCTAGTCCAAAAGAGGGCCACTTTCAAGAGACATTCAACAAAATGAAAAATACTGTTGAGGTAAGGTTACTTTT | ||
| >520999_BRCA2_15 | TTTTTGATGTCTGACAAAAAATAAGTTTTTGCATTCTAGTGATAATATACAATACACATAAATTTTTATCTTACAGTCAGAAATGAAGAAGCATCTGAAACTGTATTT | ||
| >520999_BRCA2_150 | AAATACTTTTGGAAACATAAATATGTGGGTTTGCAATTTATAAAGCAGCTTTTCCACTTATTTTCTTAGAATATTGACATACTTTGCAATGAAGCAGAAA | ||
| >520999_BRCA2_151 | AGAAAACAAGCTTATGCATATACTGCATGCAAATGATCCCAAGTGGTCCACCCCAACTAAAGACTGTACTTCAGGGCCGTACACTGCTCAAATCATTCCT | ||
| >520999_BRCA2_152 | TCAGGGCCGTACACTGCTCAAATCATTCCTGGTACAGGAAACAAGCTTCTGGTAAGTTAATGTAAACTCAAGGAATATTATAAGAAGTATATATGGA | ||
| >520999_BRCA2_153 | TGATTTAGTTTTTTATGTTACTACATAATTATGATAGGCTACGTTTTCATTTTTTTATCAGATGTCTTCTCCTAATTGTGAGATATATTATCAAAGTCCTTTATCACTTTGTATGGCCA | ||
| >520999_BRCA2_154 | TTTATCACTTTGTATGGCCAAAAGGAAGTCTGTTTCCACACCTGTCTCAGCCCAGATGACTTCAAAGTCTTGTAAAGGGGAGAAAGAGATTGATGACCAA | ||
| >520999_BRCA2_155 | TGTAAAGGGGAGAAAGAGATTGATGACCAAAAGAACTGCAAAAAGAGAAGAGCCTTGGATTTCTTGAGTAGACTGCCTTTACCTCCACCTGTTAGTCCCAT | ||
| >520999_BRCA2_156 | CCTGTTAGTCCCATTTGTACATTTGTTTCTCCGGCTGCACAGAAGGCATTTCAGCCACCAAGGAGTTGTGGCACCAAATACGAAACACCCATAAAGAAAAA | ||
| >520999_BRCA2_157 | GCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTCCTCAGATGACTCCATTTAAAAAATTCAATGAAATTTCTCTTTTGGAAAGTAATT | ||
| >520999_BRCA2_158 | TCTCTTTTGGAAAGTAATTCAATAGCTGACGAAGAACTTGCATTGATAAATACCCAAGCTCTTTTGTCTGGTTCAACAGGAGAAAAACAATTTATATCTG | ||
| >520999_BRCA2_159 | AGAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAGTTCAGAAGATTATCTCAGACTGAAACGACGTTGTACTACATCTCTGATC | ||
| >520999_BRCA2_16 | AAACTGTATTTCCTCATGATACTACTGCTGTAAGTAAATATGACATTGATTAGACTGTTGAAATTGCTAACAATTTTGGAATGCCTTGTTAAATTATTTATCTTACATTTTTAATTT | ||
| >520999_BRCA2_160 | TCTGATCAAAGAACAGGAGAGTTCCCAGGCCAGTACGGAAGAATGTGAGAAAAATAAGCAGGACACAATTACAACTAAAAAATATATCTAAGCATTTGCAAAGGCGACAATAAATTATT | ||
| >520999_BRCA2_161 | ACACAATTACAACTAAAAAATATATCTAAGCATTTGCAAAGGCGACAATAAATTATTGACGCTTAACCTTTCCAGTTTATAAGACTGGAATATAATTTCAA | ||
| >520999_BRCA2_17 | TTTTATACTAGTGATTTTAAACTATAATTTTTGCAGAATGTGAAAAGCTATTTTTCCAATCATGATGAAAGTCTGAAGAAAAATGATAGATTTA | ||
| >520999_BRCA2_18 | AAAAATGATAGATTTATCGCTTCTGTGACAGACAGTGAAAACACAAATCAAAGAGAAGCTGCAAGTCATGGTAA | ||
| >520999_BRCA2_19 | ATGGTAAGTCCTCTGTTTAGTTGAACTACAGGTTTTTTTGTTGTTGTTGTTTTGATTTTTTTTTTTTGAGGTGGAGTCTTGCT | ||
| >520999_BRCA2_20 | AATAATATAAATTATATGGCTTATAAAATATTAATGTGCTTCTGTTTTATACTTTAACAGGATTTGGAAAAACATCAGGGAATTCATTTAAAGTAAATA | ||
| >520999_BRCA2_21 | ATTTAAAGTAAATAGCTGCAAAGACCACATTGGAAAGTCAATGCCAAATGTCCTAGAAGATGAAGTATATGAAA | ||
| >520999_BRCA2_22 | TATATGAAACAGTTGTAGATACCTCTGAAGAAGATAGTTTTTCATTATGTTTTTCTAAATGTAGAACAAAAAATCTACAAAAAGTAAGAACTAGCAAGACTAGGAAAAAAATTTTCCATGAAGCAAA | ||
| >520999_BRCA2_23 | AAAAAAATTTTCCATGAAGCAAACGCTGATGAATGTGAAAAATCTAAAAACCAAGTGAAAGAAAAATACTCATTTGTATCTGAAGTGGAACCAAATGATACTGATCCATTAGATTCAAATGTAGCAAAT | ||
| >520999_BRCA2_24 | ATTAGATTCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTCCAAGGAAGTTGTACCGTCTTTGGC | ||
| >520999_BRCA2_25 | TGGAAGTGACAAAATCTCCAAGGAAGTTGTACCGTCTTTGGCCTGTGAATGGTCTCAACTAACCCTTTCAGGTCTAAATGGAGCCCAGATGGAGAAAATA | ||
| >520999_BRCA2_26 | TAAATGGAGCCCAGATGGAGAAAATACCCCTATTGCATATTTCTTCATGTGACCAAAATATTTCAGAAAAA | ||
| >520999_BRCA2_27 | AAAATATTTCAGAAAAAGACCTATTAGACACAGAGAACAAAAGAAAGAAAGATTTTCTTACTTCAGAGAATTCTTTGCCACGTATTTCTAGCCTACCAAAATCAG | ||
| >520999_BRCA2_28 | TACCAAAATCAGAGAAGCCATTAAATGAGGAAACAGTGGTAAATAAGAGAGATGAAGAGCAGCATCTTGAATCTCATACAGACTGCATTCTTGCAGTAAA | ||
| >520999_BRCA2_29 | CTCATACAGACTGCATTCTTGCAGTAAAGCAGGCAATATCTGGAACTTCTCCAGTGGCTTCTTCATTTCAGGGTATCAAAAAGTCTATATTCAGAATAA | ||
| >520999_BRCA2_30 | ATTTCAGGGTATCAAAAAGTCTATATTCAGAATAAGAGAATCACCTAAAGAGACTTTCAATGCAAGTTTTTCAGGTCATATGACTGATCCAAACTTTAAAAAA | ||
| >520999_BRCA2_31 | ATATGACTGATCCAAACTTTAAAAAAGAAACTGAAGCCTCTGAAAGTGGACTGGAAATACATACTGTTTGCTCACAGAAGGAGGACTCCTTATGTCCAAAT | ||
| >520999_BRCA2_32 | ACTCCTTATGTCCAAATTTAATTGATAATGGAAGCTGGCCAGCCACCACCACACAGAATTCTGTAGCTTTGAAGAATGCAG | ||
| >520999_BRCA2_33 | AGAATTCTGTAGCTTTGAAGAATGCAGGTTTAATATCCACTTTGAAAAAGAAAACAAATAAGTTTATTTATGCTATACATGATGAAACATCTTATAAAGGAAAAAAAATA | ||
| >520999_BRCA2_34 | TTTGAAAAAGAAAACAAATAAGTTTATTTATGCTATACATGATGAAACATCTTATAAAGGAAAAAAAATACCGAAAGACCAAAAATCAGAACTAATTAA | ||
| >520999_BRCA2_35 | AAAAATCAGAACTAATTAACTGTTCAGCCCAGTTTGAAGCAAATGCTTTTGAAGCACCACTTACATTTGCAAATGCTGATTCAGGTACCTCTGTCTTTTTTTTTTTGTAAATA | ||
| >520999_BRCA2_36 | TTTTTATGTTTAGGTTTATTGCATTCTTCTGTGAAAAGAAGCTGTTCACAGAATGATTCTGAAGAACCAACTTTGTCCTTAACTAGCTCTTTT | ||
| >520999_BRCA2_37 | TTAACTAGCTCTTTTGGGACAATTCTGAGGAAATGTTCTAGAAATGAAACATGTTCTAATAATACAGTAAT | ||
| >520999_BRCA2_38 | TAATAATACAGTAATCTCTCAGGATCTTGATTATAAAGAAGCAAAATGTAATAAGGAAAAACTACAGTTATTTATTACCCCAGAAGCTGATTCTCTGTCATGCCTGCAGGAA | ||
| >520999_BRCA2_39 | CCTGCAGGAAGGACAGTGTGAAAATGATCCAAAAAGCAAAAAAGTTTCAGATATAAAAGAAGAGGTCTTGGCTGCAGCATGTCACCCAGTACAACATTCAAAAGT | ||
| >520999_BRCA2_40 | ATTCAAAAGTGGAATACAGTGATACTGACTTTCAATCCCAGAAAAGTCTTTTATATGATCATGAAAATGCCAGCACTCTTATTTTAACTCCTACTT | ||
| >520999_BRCA2_41 | TTATTTTAACTCCTACTTCCAAGGATGTTCTGTCAAACCTAGTCATGATTTCTAGAGGCAAAGAATCATACAAAAT | ||
| >520999_BRCA2_42 | AATCATACAAAATGTCAGACAAGCTCAAAGGTAACAATTATGAATCTGATGTTGAATTAACCAAAAATATT | ||
| >520999_BRCA2_43 | AATTATGAATCTGATGTTGAATTAACCAAAAATATTCCCATGGAAAAGAATCAAGATGTATGTGCTTTAAATGAAAATTATAAAAA | ||
| >520999_BRCA2_44 | TTTAAATGAAAATTATAAAAACGTTGAGCTGTTGCCACCTGAAAAATACATGAGAGTAGCATCACCTTCAAGAAA | ||
| >520999_BRCA2_45 | TTCAAGAAAGGTACAATTCAACCAAAACACAAATCTAAGAGTAATCCAAAAAAATCAAGAAGAAACTACTTCAATTTCAAAAATAA | ||
| >520999_BRCA2_46 | AAAAAAATCAAGAAGAAACTACTTCAATTTCAAAAATAACTGTCAATCCAGACTCTGAAGAACTTTTCTCAGACAATGAGAATAATTTTGTCTT | ||
| >520999_BRCA2_47 | AATAATTTTGTCTTCCAAGTAGCTAATGAAAGGAATAATCTTGCTTTAGGAAATACTAAGGAACTTCATGAAACAGACTTGACTTGTGTAAA | ||
| >520999_BRCA2_48 | TTGTGTAAACGAACCCATTTTCAAGAACTCTACCATGGTTTTATATGGAGACACAGGTGATAAACAAGCAACCCAAGTGTCAATTAAAAAA | ||
| >520999_BRCA2_49 | CAAGTGTCAATTAAAAAAGATTTGGTTTATGTTCTTGCAGAGGAGAACAAAAATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTCAAGATTTAAAAT | ||
| >520999_BRCA2_50 | ATTTAAAATCGGACATCTCCTTGAATATAGATAAAATACCAGAAAAAAATAATGATTACATGAACAAATGGGCAGGACTCTTAGGTCCAATTTCAAATCACAGTTTTGGAGGTA | ||
| >520999_BRCA2_51 | AATTTCAAATCACAGTTTTGGAGGTAGCTTCAGAACAGCTTCAAATAAGGAAATCAAGCTCTCTGAACATAACATTAAGAAGAGCAAAATGTTCTTCAAAGATATT | ||
| >520999_BRCA2_52 | AAATGTTCTTCAAAGATATTGAAGAACAATATCCTACTAGTTTAGCTTGTGTTGAAATTGTAAATACCTTGGCATTAGATAATCAAAAGAAACTGAGCAAGCCTCAGTCAATTAATA | ||
| >520999_BRCA2_53 | TCAATTAATACTGTATCTGCACATTTACAGAGTAGTGTAGTTGTTTCTGATTGTAAAAATAGTCATATAACCCCTCAGATGTTATTTTCCAAGCAGGATTTTAATTCAAACCATAATTTAA | ||
| >520999_BRCA2_54 | ATTTTAATTCAAACCATAATTTAACACCTAGCCAAAAGGCAGAAATTACAGAACTTTCTACTATATTAGAAGAATCAGGAAGTCAGTTTGAATTTACTCAGTTTAGAAAA | ||
| >520999_BRCA2_55 | TTTGAATTTACTCAGTTTAGAAAACCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGATGACTATCTTAAAGACCACTTCTGA | ||
| >520999_BRCA2_56 | ACTTCTGAGGAATGCAGAGATGCTGATCTTCATGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAATTTGAAGGTACAGTTGAAATTAAA | ||
| >520999_BRCA2_57 | AGCAAGCAATTTGAAGGTACAGTTGAAATTAAACGGAAGTTTGCTGGCCTGTTGAAAAATGACTGTAACAAAAGTGCTTCTGGTTATTTAACAGATGAAAATGAAGTGGGGTTTAGGGGCTTTTATT | ||
| >520999_BRCA2_58 | GGCTTTTATTCTGCTCATGGCACAAAACTGAATGTTTCTACTGAAGCTCTGCAAAAAGCTGTGAAACTGTTTAGTGATATTGAGAATATTA | ||
| >520999_BRCA2_59 | AAAAAGCTGTGAAACTGTTTAGTGATATTGAGAATATTAGTGAGGAAACTTCTGCAGAGGTACATCCAATAAGTTTATCTTCAAGTAAATGTCATGATTCTGTT | ||
| >520999_BRCA2_60 | AATAAGTTTATCTTCAAGTAAATGTCATGATTCTGTTGTTTCAATGTTTAAGATAGAAAATCATAATGATAAAACTGTAAGTGAAAAAAATAATAAAT | ||
| >520999_BRCA2_61 | TTGTTTCAATGTTTAAGATAGAAAATCATAATGATAAAACTGTAAGTGAAAAAAATAATAAATGCCAACTGATATTACAAAATAATATTGAAAT | ||
| >520999_BRCA2_62 | AAAAAAATAATAAATGCCAACTGATATTACAAAATAATATTGAAATGACTACTGGCACTTTTGTTGAAGAAATTACTGAAAATTACAAGAGAAATACTGAAAATGAAGATAACAAATATA | ||
| >520999_BRCA2_63 | AAATTACTGAAAATTACAAGAGAAATACTGAAAATGAAGATAACAAATATACTGCTGCCAGTAGAAATTCTCATAACTTAGAATTTGATGGCAGTGATTCAAGTAAAAATGATACT | ||
| >520999_BRCA2_64 | ATTCAAGTAAAAATGATACTGTTTGTATTCATAAAGATGAAACGGACTTGCTATTTACTGATCAGCACAACATATGTCTTAAATTATCTGGCCAGTTTATGAA | ||
| >520999_BRCA2_65 | TTAAATTATCTGGCCAGTTTATGAAGGAGGGAAACACTCAGATTAAAGAAGATTTGTCAGATTTAACTTTTTTGGAAGTTG | ||
| >520999_BRCA2_66 | ATTTAACTTTTTTGGAAGTTGCGAAAGCTCAAGAAGCATGTCATGGTAATACTTCAAATAAAGAACAGTTAACTGCTACTAAAACGGAGCAAAATATAAAAGATTTT | ||
| >520999_BRCA2_67 | AAAATATAAAAGATTTTGAGACTTCTGATACATTTTTTCAGACTGCAAGTGGGAAAAATATTAGTGTCGCCAAAGAGTCATTTAATAAAATTGTAAATTTCTTT | ||
| >520999_BRCA2_68 | ATTTAATAAAATTGTAAATTTCTTTGATCAGAAACCAGAAGAATTGCATAACTTTTCCTTAAATTCTGAATTACATTCTGACATAAGAAAGAACAAAAT | ||
| >520999_BRCA2_69 | TTAAATTCTGAATTACATTCTGACATAAGAAAGAACAAAATGGACATTCTAAGTTATGAGGAAACAGACATAGTTAAACACAAAATACTGAAAGAAA | ||
| >520999_BRCA2_70 | TTAAACACAAAATACTGAAAGAAAGTGTCCCAGTTGGTACTGGAAATCAACTAGTGACCTTCCAGGGACAACCCGAACGTGATGAAAAGATCAAAGAACCTACTCTATTG | ||
| >520999_BRCA2_71 | AAAAGATCAAAGAACCTACTCTATTGGGTTTTCATACAGCTAGCGGGAAAAAAGTTAAAATTGCAAAGGAATCTTTGGACAAAGTGAAAAACCTTTTTGATGAAAAA | ||
| >520999_BRCA2_72 | AAAAACCTTTTTGATGAAAAAGAGCAAGGTACTAGTGAAATCACCAGTTTTAGCCATCAATGGGCAAAGACCCTAAAGTACAGAGAGGCCTGTAAAGACCTTGAATTA | ||
| >520999_BRCA2_73 | TAAAGACCTTGAATTAGCATGTGAGACCATTGAGATCACAGCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAACCTTGTTTCTATT | ||
| >520999_BRCA2_74 | AATAATGATAAAAACCTTGTTTCTATTGAGACTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAAAATCTCAAAACATCAAAAAGTATCTTTTTGAAAGTTAAA | ||
| >520999_BRCA2_75 | ATAATTTATGTAGACAAACTGAAAATCTCAAAACATCAAAAAGTATCTTTTTGAAAGTTAAAGTACATGAAAATGTAGAAAAAGAAACAGCAAAAAGTCCTGCAACTT | ||
| >520999_BRCA2_76 | AAAAAGTCCTGCAACTTGTTACACAAATCAGTCCCCTTATTCAGTCATTGAAAATTCAGCCTTAGCTTTTTACACAAGTTGTAGTAGAAAAA | ||
| >520999_BRCA2_77 | TTTTTACACAAGTTGTAGTAGAAAAACTTCTGTGAGTCAGACTTCATTACTTGAAGCAAAAAAATGGCTTAGAGAAGGAATATTT | ||
| >520999_BRCA2_78 | AAAAAAATGGCTTAGAGAAGGAATATTTGATGGTCAACCAGAAAGAATAAATACTGCAGATTATGTAGGAAATTATTTGTATGAAAATAATTCAAA | ||
| >520999_BRCA2_79 | AAATTATTTGTATGAAAATAATTCAAACAGTACTATAGCTGAAAATGACAAAAATCATCTCTCCGAAAAACAAGATACTTATTTAAGTAACAGTAGCATGTCTAACA | ||
| >520999_BRCA2_80 | ATGTCTAACAGCTATTCCTACCATTCTGATGAGGTATATAATGATTCAGGATATCTCTCAAAAAATAAACTTGATTCTGGTATT | ||
| >520999_BRCA2_81 | AAAAAATAAACTTGATTCTGGTATTGAGCCAGTATTGAAGAATGTTGAAGATCAAAAAAACACTAGTTTTTCCAAAGTAATATCCAATGTAAAA | ||
| >520999_BRCA2_82 | AAAAAAACACTAGTTTTTCCAAAGTAATATCCAATGTAAAAGATGCAAATGCATACCCACAAACTGTAAATGAAGATATTTGCGTTGA | ||
| >520999_BRCA2_83 | TAAATGAAGATATTTGCGTTGAGGAACTTGTGACTAGCTCTTCACCCTGCAAAAATAAAAATGCAGCCATTAAATTGTCCATATCTAATAGTAATAATTTTGAGGTAGGGCCA | ||
| >520999_BRCA2_84 | AAAAATAAAAATGCAGCCATTAAATTGTCCATATCTAATAGTAATAATTTTGAGGTAGGGCCACCTGCATTTAGGATAGCCAGTGGTAAAATCGTTT | ||
| >520999_BRCA2_85 | TAAAATCGTTTGTGTTTCACATGAAACAATTAAAAAAGTGAAAGACATATTTACAGACAGTTTCAGTAAAGTAATTAAGGAAAACAACGAGAATAAATCAAAAATTT | ||
| >520999_BRCA2_86 | AATAAATCAAAAATTTGCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAGGATATTCTTCATAACTCTCTAGATAAT | ||
| >520999_BRCA2_87 | ATATTCTTCATAACTCTCTAGATAATGATGAATGTAGCACGCATTCACATAAGGTTTTTGCTGACATTCAGAGTGAAGAAATTTTACAACATAACCAAAATAT | ||
| >520999_BRCA2_88 | AAATTTTACAACATAACCAAAATATGTCTGGATTGGAGAAAGTTTCTAAAATATCACCTTGTGATGTTAGTTTGGAAACTTCAGATATATGTAAATGTAGTATA | ||
| >520999_BRCA2_89 | ATATATGTAAATGTAGTATAGGGAAGCTTCATAAGTCAGTCTCATCTGCAAATACTTGTGGGATTTTTAGCACAGCAAGTGGAAAATCTGTCCA | ||
| >520999_BRCA2_90 | AAAATCTGTCCAGGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAGAAGATAGTACCAAGCAAGTCTTTTCCAAAGTATTGTTTAAAAGTAAC | ||
| >520999_BRCA2_91 | TTTTCCAAAGTATTGTTTAAAAGTAACGAACATTCAGACCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAATATCCCAAAAAGGCTTTTCATATAAT | ||
| >520999_BRCA2_92 | ATTTAATATCCCAAAAAGGCTTTTCATATAATGTGGTAAATTCATCTGCTTTCTCTGGATTTAGTACAGCAAGTGGAAAGCAAGTTTCCATTTTAGAAAGTT | ||
| >520999_BRCA2_93 | ATTTTAGAAAGTTCCTTACACAAAGTTAAGGGAGTGTTAGAGGAATTTGATTTAATCAGAACTGAGCATAGTCTTCACTATTCACCTACGTCTAGACAAAATGTATCAAAAATACTT | ||
| >520999_BRCA2_94 | AAATGTATCAAAAATACTTCCTCGTGTTGATAAGAGAAACCCAGAGCACTGTGTAAACTCAGAAATGGAAAAAACCTGCAGTAAAGAATTTAAATTATCAAATAACTTAAAT | ||
| >520999_BRCA2_95 | AAAAAACCTGCAGTAAAGAATTTAAATTATCAAATAACTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTTCTCCATATCTCTCTCAATTT | ||
| >520999_BRCA2_96 | TATTAAAGTTTCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGGAACCAAAGTGTCACTTGTTGAGAACATTCATGTTTTGGGAAAA | ||
| >520999_BRCA2_97 | ATTCATGTTTTGGGAAAAGAACAGGCTTCACCTAAAAACGTAAAAATGGAAATTGGTAAAACTGAAACTTTTTCTGATGTTCCTGTGAAAACAAATATAGAAGTTT | ||
| >520999_BRCA2_98 | AAAACAAATATAGAAGTTTGTTCTACTTACTCCAAAGATTCAGAAAACTACTTTGAAACAGAAGCAGTAGAAATTGCTAAAGCTTTTATGGAAGATGATGAACTGACAGATTCTAAA | ||
| >520999_BRCA2_99 | AGATTCTAAACTGCCAAGTCATGCCACACATTCTCTTTTTACATGTCCCGAAAATGAGGAAATGGTTTTGTCAAATTCAAGAATTGGAAAAAGAAGAGGAGAGCC | ||
| >520999_CYP2D6_01 | 97 | GCTGGGGACTAGGTACCCCATTCTAGCGGGGCACAGCACAAAGCTCATAGGGGGATGGGGTCACCAGGAAAGCAAAGACACCATGGTGGCTGGGCCGGGGCTGT | |
| >520999_CYP2D6_02 | 98 | TGGCTGGGCCGGGGCTGTCCAGTGGGCACCGAGAAGCTGAAGTGCTGCAGCAGGGAGGTGAAGAAGAGGAAGAGCTCCATGCGGGCCAGGGGCTCCCCGAGGCATGCACGGCGGCCTGTGGGGAGGGGAGGGGC | |
| >520999_CYP2D6_03 | 99 | GAGCCGGGCTCCCCACAGGCACCTGCTGAGAAAGGCAGGAAGGCCTCCGGCTTCACAAAGTGGCCCTGGGCATCCAGGAAGTGTTCGGGGTGGAAGCGGAAGGGCTT | |
| >520999_CYP2D6_04 | 100 | CGGAAGGGCTTCTCCCAGACGGCCTCATCCTTCAGCACCGATGACAGGTTGGTGATGAGTGTCGTTCCCTGGGCAGAAGATGCAGGGTGAGA | |
| >520999_CYP2D6_05 | 101 | GGGTGAGGAGGGCGCCAGGCCTACCTTAGGGATGCGGAAGCCCTGTACTTCGATGTCACGGGATGTCATATGGGTCACACCCAGGGGGACGATGTCCCCAAAGCGCTGCACCT | |
| >520999_CYP2D6_06 | 102 | ATGTCCCCAAAGCGCTGCACCTCATGAATCACGGCAGTGGTGTAGGGCATGTGAGCCTGGTCACCCATCTCTGGTCGCCGCACCTGCCCTATCACGTCGTCGATCTCCTGTTGGACAC | |
| >520999_CYP2D6_07 | 103 | GCATGTGAGCCTGGTCACCCATCTCTGGTCGCCGCACCTGCCCTATCACGTCGTCGATCTCCTGTTGGACACGGCCTGGACAGACATGCGTCCCCACAATGGGTCAGCACCCAGGGG | |
| >520999_CYP2D6_08 | 104 | GCCCCCGCCTGTACCCTTCCTCCCTCGGCCCCTGCACTGTTTCCCAGATGGGCTCACGCTGCACATCCGGATGTAGGATCATGAGCAGGAGGCCCCAGGCCAGCGT | |
| >520999_CYP2D6_09 | 105 | TAGGATCATGAGCAGGAGGCCCCAGGCCAGCGTGGTCGAGGTGGTCACCATCCCGGCAGAGAACAGGTCAGCCACCACTATGCACAGGTTCTCATCATTGAAGCTGCTCTCAGG | |
| >520999_CYP2D6_10 | 106 | CACCACTATGCACAGGTTCTCATCATTGAAGCTGCTCTCAGGGTTCCCCTTGGCCTGAGCAGGGCCGA | |
| >520999_CYP2D6_11 | 107 | AACCCACCACCCTTGCCCCCCACCGTGGCAGCCACTCTCACCTTCTCCATCTCTGCCAGGAAGGCCTCAGTCAGGTCTCGGGGGGGCTGGGCTGGGTCCCA | |
| >520999_CYP2D6_12 | 108 | CAGGAAGGCCTCAGTCAGGTCTCGGGGGGGCTGGGCTGGGTCCCAGGTCATCCTGTGCTCAGTTAGCAGCTCATCCAGCTGGGTCAGGAAAGCCTTTTGGAAGCGTAGGACCTTGCCAGCCAGCGCTGG | |
| >520999_CYP2D6_13 | 109 | CAGCCAGCGCTGGGATATGCAGGAGGACGGGGACAGCATTCAGCACCTACACCAGACAGAACGGGGTCTCAATCCCTCCTGTGCTCTGCGTTCACCTG | |
| >520999_CYP2D6_14 | 110 | TCCGCACCTCGCGCAGAAAGCCCGACTCCTCCTTCAGTCCCTCCTGAGCTAGGTCCAGCAGCCTGAGGAAGCGAGGGTCGTCGTACTCGAAGCGGCGCCCGCAGGTGAGGGAGGCGATCACGTTGCTCACGGCTTTGTCCAA | |
| >520999_CYP2D6_15 | 111 | GGCGCCCGCAGGTGAGGGAGGCGATCACGTTGCTCACGGCTTTGTCCAAGAGACCGTTGGGGCGAAAGGGGCGTCCTGGGGGTGGGAGATGCGGGTAAGGG | |
| >520999_CYP2D6_16 | 112 | CGTCCCCCGCCTTCCCAGTTCCCGCTTTGTGCCCTTCTGCCCATCACCCACCGGAGTGGTTGGCGAAGGCGGCACAAAGGCAGGCGGCCTCCTCGGTCACCCA | |
| >520999_CYP2D6_17 | 113 | GCGAAGGCGGCACAAAGGCAGGCGGCCTCCTCGGTCACCCACTGCTCCAGCGACTTCTTGCCCAGGCCCAAGTTGCGCAAGGTGGAGACGGAGAAGCGCCTCTGCTCGCGCCACGCGGGCCCATAGCG | |
| >520999_CYP2D6_18 | 114 | AGACGGAGAAGCGCCTCTGCTCGCGCCACGCGGGCCCATAGCGCGCCAGGAACACCCCTGGGGGTGGGACGGGCACGTGCGCGTGGCCATGAAGG | |
| >520999_CYP2D6_19 | 115 | TCCCCACCGCTGCTTGCCTTGGGAACGCGGCCCGAAACCCAGGATCTGGGTGATGGGCACAGGCGGGCGGTCGGCGGTGTCCTCGCCGTGGGTCACCAGCGCCTCGCGCACGGCCGC | |
| >520999_CYP2D6_20 | 116 | GGCCCGAAACCCAGGATCTGGGTGATGGGCACAGGCGGGCGGTCGGCGGTGTCCTCGCCGTGGGTCACCAGCGCCTCGCGCACGGCCGCCAGCCCATTGAGCACGACCACCGGCGTCCAGGCCAG | |
| >520999_CYP2D6_21 | 117 | CAGGCCAGCTGCAGGCTGAACACGTCCCCGAAGCGGCGCCGCAACTGCAGAGGGAGGGTCAGGGCCTCTTGTCAAGCCAGGATCC | |
| >520999_CYP2D6_22 | 118 | CCACCACCCATGTTTGCTGGTGGTGGGGCATCCTCAGGACCTCTGCCGCCCTCCAGGACCTCCTCCCTCACCTGGTCGAAGCAGTATGGTGTGTT | |
| >520999_CYP2D6_23 | 119 | AGTATGGTGTGTTCTGGAAGTCCACATGCAGCAGGTTGCCCAGCCCGGGCAGTGGCAGGGGGCCTGGTGGGTAGCGTGCAGCCCAGCGTTGGCGCCGGTGCATCA | |
| >520999_CYP2D6_24 | 120 | CCGGTGCATCAGGTCCACCAGGAGCAGGAAGATGGCCACTATCACGGCCAGGGGCACCAGTGCTTCTAGCCCCATACCTGCCTCACTAC | |
| >520999_DPYD_01 | TTTAAATTTCACACATAGCAACAGAAAATGCTTTCTGCCGTAAAAACAAGAAGAAACATGTCTCATAGCATTCTAATTCCAGCAGGATTCTTACCTGGTAGCCAGAATCATTA | ||
| >520999_DPYD_02 | ATTCTTACCTGGTAGCCAGAATCATTACAGGTCATGTAGCATTTACCACAGTTGATACACATTTCTTCATCAATCATAGCCACAACTTGCTCTACGTTGCTCAATTC | ||
| >520999_DPYD_03 | TACGTTGCTCAATTCACCAAATGTTCCAAGGTACTGCAGTGCTTTTCCTATTACATCCTAAAAATA | ||
| >520999_DPYD_04 | TAAAAATAGCCACTGAATTACTTAGCAAGCTCATTTTAAAACATTTTCATGTAATAATTAATAT | ||
| >520999_DPYD_05 | AAGAATAACACAGGAGATTTAAGCACATACCTTGTCCATGAGTTCAGCTATACGTGGAACTGGTTTCCCTTT | ||
| >520999_DPYD_06 | GTTTCCCTTTCTGGTGACTCACAGTAGCTGGACTCTGTCCATCCCAGTCTTGTAGTTCTTCAATGCTTTTCAGATAAAGCAGGGCTTTGAGGCCAGTGCAGTAGTCTTCGATCACAGTGAAAT | ||
| >520999_DPYD_07 | ATCACAGTGAAATCCTGATTCTGAATGGCACTGCATACCTAGAAAAGACAGAGCAGTCAACCAA | ||
| >520999_DPYD_08 | TGATTTTTCAGCAACCTCCAAGAAAGCACAATGCAAGAAGTGGGCAACACCTACCAGACACTCCTCCATATGTAGTTCGCTTTGCAATCCCCACTGCTGGCCAAGGTGT | ||
| >520999_DPYD_09 | GCCAAGGTGTGCCATCAGATTTTAATCCCATCAGACCTGAGACAGTGTTGGTGGCTGTAACGCCATTGGCACCACCTATGCAAGACACATCAACATTTTCAT | ||
| >520999_DPYD_10 | TTTTAGATGTTAAATCACACTTACGTTGTCTGGAAAGTCAGCCTTTAGTTCAGTGACACTTTGACACCAATATGCAGCCGTTTTCTCACTGATGAGCTCAATATTCA | ||
| >520999_DPYD_11 | AGCTCAATATTCAGAAAGGAGCTTTGTCCAGGGCCATACATGGGGCCAGAGGTGGTTCCCCGGATGATTCTGGGGGAAACATTTGTCACAATGTCCTGATGAAAGAGTAAAGATATT | ||
| >520999_DPYD_12 | TTACCTTATCAAGAGAGAAAGTTTTGGTGAGGGCAAAACCCCATCCAGCTTCAAAAGCTCTTCGAATCATTGATGTGCTGGTGGCTGGAGTTGCGCTAGCAAGACCAAAAGGATTTATAAA | ||
| >520999_DPYD_13 | ATTTATAAACTTCAATCCGGCCATTTCTACACTAATGTCCACCAGATCAATAGGAGTGTAAAAGAGGGGTAGTTCAGGCTTGGCAGAAACGGAAGCTCCATATTGTGACTGCAAAATACAAA | ||
| >520999_DPYD_14 | AAATAAAAAAATACATTATAAATAAATATATTTAAGAGTAAACTACTCACCTATTCCAATGAAAGCAGCTTTGTAGCCTTTTTCTTTCAAAGTGCTAAGA | ||
| >520999_DPYD_15 | TTTTTCTTTCAAAGTGCTAAGAGTCATTTCATTCACTGAAAGGCTTTTACCGCAAATTATCTATAAGAAACAATATTTTGCATAAGAAAATTTGGCATATGATTAATTAAAATCTTAATATTTAACATCAAAAAGAATGAAAA | ||
| >520999_DPYD_16 | TTACTCCTTTCTTTTTGAGCAGTACACAGATAGGTGTTTTTTTCATTTACCTTTACACCAAGGTCCTTCATTAGCTCAATCTCAAAATTCACTACATCATACGGCAGCC | ||
| >520999_DPYD_17 | AAAATTCACTACATCATACGGCAGCCGGAACTGAGGAATTTCAGAAGTACTGAAAAGAAAGGAGAAAGAAAAACA | ||
| >520999_DPYD_18 | GGGATGTCACGTGTATATCATACATACCTCAGTAGCAAATTGCTGCAATCCACCAATATTAATGGGTCCCTCTTCAGTGGCATATAAATTGCATCCACCTACACAAAGATCAGAGGTTGGA | ||
| >520999_DPYD_19 | AGGTTGGACATACCATTCCACAAGTCAGACCAAGTGGGTTGTCAGAAAATATCATCTTAGCAGCTCCATAATAGTTCTGCAAAATTAATACAAAATAAAATTTTACTACTTAAAAATATACTTTAAA | ||
| >520999_DPYD_20 | ATAATAGAGAACAAGATCAAATACTGTTATTTTCATTTGCAGAGTTAAATCTGAATTTACCTTGTTTGCAATACTTGTGATGAATGATTTAATATCAAGATTAGTTGGACAGCTCTTCTGACACGGG | ||
| >520999_DPYD_21 | ATTTAATATCAAGATTAGTTGGACAGCTCTTCTGACACGGGGCATCTGCACATTTCAGGCATCTAGGAAATAAAATAACTATGTTAAGAAACTACAAGATAAGTGAGATAATCTATTTT | ||
| >520999_DPYD_22 | TAATTTTCAGCATGAAATAGTGTATCAGTGGTACTTACAAAGCAGTTCTTATCAGGATTTCTTTTCCAATGTTTCTTGTCTAATTTCTTGGCCGAAGTGGAACGCAGAGTTGCATG | ||
| >520999_DPYD_23 | AACGCAGAGTTGCATGAGTTTGTGTTCGAGGATTTAAAGCCAGGATACTCTAAAGACAGCATAAACAATGTGTAAATATAT | ||
| >520999_EGFR_01 | CCCTTGTCTCTGTGTTCTTGTCCCCCCCAGCTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAA | ||
| >520999_EGFR_02 | TGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACGGTGTATAAGGTAAGGTCCCTGGCACAGGCCTCTGGGCTGGGCCGCAGGGCCTCTCATGGTCTGGTGGGGAG | ||
| >520999_EGFR_03 | GTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTC | ||
| >520999_EGFR_04 | AATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGTGAGTTTCTGCTTTGCTGTGTGGGGGTCCATGG | ||
| >520999_EGFR_05 | CACACTGACGTGCCTCTCCCTCCCTCCAGGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAG | ||
| >520999_EGFR_06 | CACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATC | ||
| >520999_EGFR_07 | AATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGTAATCAGGGAAGGGAGATACGGGGAGGGGAGATAAGGAGCCAGGATCCTCACATGCGGTCTGCG | ||
| >520999_EGFR_08 | TCCCTCACAGCAGGGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAACACCGCAGCATGTCAA | ||
| >520999_EGFR_09 | ACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAGTAAGGAGGTGGCTTTAGGTCAGCCAGCATTTTCCTGACACCAGG | ||
| >520999_ERBB2_01 | CCTTGTCCCCAGGAAGCATACGTGATGGCTGGTGTGGGCTCCCCATATGTCTCCCGCCTTCTGGGCATCTGCCTGACATCCACGGTGCAGCTGGTGACACA | ||
| >520999_ERBB2_02 | CTGGTGACACAGCTTATGCCCTATGGCTGCCTCTTAGACCATGTCCGGGAAAACCGCGGACGCCTGGGCTCCCAGGACCTGCTGAACTGGTGTATGCAGATTGCCAAGGTATGCACCTGGGCTCTTTGCAG | ||
| >520999_FOXL2_01 | AAATAAAGAAGTCCGTGTTAAAAGAAGGAACACAAAATTTATTCGGGAATCGACAAGGCAAAAAACAAAAACAAAACAAAAAAACACAACAAAAGTGCTCTAGTTCTCTCTAGAAAATTTGGTCCCCCAAA | ||
| >520999_FOXL2_02 | AAAATTTGGTCCCCCAAACAACAAGCTACAGTACAACCAGGTCTATGGTTTTCGGGCCGGGCCGGCTCCGGGCCACAAGACCGCCTAGGTCGGCCGCTGCCGGGTTTCACATTTCTCCTTC | ||
| >520999_FOXL2_03 | CCGCTGCCGGGTTTCACATTTCTCCTTCCCAAGGTGGGAGAAGCAGGAGGTTTGAAAAACAAAAAGCAGGGAGGACGAGCCGTCCCAGCAGCGTGGGCCAGGCAGGCAGT | ||
| >520999_FOXL2_04 | CAGCAGCGTGGGCCAGGCAGGCAGTGATCTCCCTGCCTCCAGGACTTGCATCGAAAGATCCGGGGACATTTGTTTTTGATTTTTTTCTTCAGATAGGGAGAGGGTGAAA | ||
| >520999_FOXL2_05 | GGACATTTGTTTTTGATTTTTTTCTTCAGATAGGGAGAGGGTGAAACTTCCCCAATTGGTAGACGAAACCATCTTTTATTGGATATATTCTCCTAACTCTGAACACAGCTTTTTGAAGGCCCAACAAAAA | ||
| >520999_FOXL2_06 | ACAGCTTTTTGAAGGCCCAACAAAAACTCTAACGCAAATCTTCCAGAAGAGAAGATGAGGAATACTAAAGCTTTTGATTTTCATATTTGGATTTA | ||
| >520999_FOXL2_07 | ATTTAGCAAACTCCAAGGCCACAATACTTCCCCGGTTGCCCGGTGAATTTTTTTTCCTTTTTTTTTTTTTAAATACTCTCTTAAAAA | ||
| >520999_FOXL2_08 | CCGGTGAATTTTTTTTCCTTTTTTTTTTTTTAAATACTCTCTTAAAAAGTTCCACCGATCTTTCAAACAAAACATTAGCCAGCCGCCGGGGCCCGGAGGTGCCCGAA | ||
| >520999_FOXL2_09 | GCCGGGGCCCGGAGGTGCCCGAAGCGACGGGACTGATAGCGGAGGAAACGCAGCCTCCCTCCGCGCCCGCCCGCGGTGTAAACCGAGTACAGGCCGTGGAGCCAGGCTGCCCCGGCTCCCGCTGGGTCCCAACCCCCGCCCCGCCTAGTGGGCCCCGCGGCAAGCG | ||
| >520999_FOXL2_10 | CCCCGGCTCCCGCTGGGTCCCAACCCCCGCCCCGCCTAGTGGGCCCCGCGGCAAGCGGCTTCTGAACAGCTTCAAGAGGGTTCGCGGAGCAAACACAC | ||
| >520999_FOXL2_11 | CAAACACACGTATTGGTCCGTCCCTTTCTCGGGCAGCGCGGTCCCGCCATCAGTCCTGTCCGGCGCGTCTAGCCATGGACTGCACGGCAGTCGGGCGGGGAACGCGGAGAGCGAGCGCACC | ||
| >520999_FOXL2_12 | GGGCGGGGAACGCGGAGAGCGAGCGCACCGACCTGTGAGAGAAGGCCAAGAGGTCTGCGCTGCCGACGCCCGGTCGCACCTCCGCCCCGGGCCCTTTCCGC | ||
| >520999_FOXL2_13 | CCGACGCCCGGTCGCACCTCCGCCCCGGGCCCTTTCCGCGGTGAATTTGGGCAGGAGACGCTGGGGCTCCGGAAAGAGACGAGCCCAGTAGAAAGCGCGCAGAGAGGCAGCTTCAGGCCAGGGGAGTGCAAGGTCA | ||
| >520999_FOXL2_14 | GCAGAGAGGCAGCTTCAGGCCAGGGGAGTGCAAGGTCACAGAGGTCAGGGAGGTGAGCACAGGAGGACATAAACTGAGGGGACAAAGAGGAGCGACAGGAGCTTA | ||
| >520999_FOXL2_15 | GACAGGAGCTTAGGAAAGCGAAAAAGCACAGAGGGACCCTGGGCGCTGGCTCCAGAGGCGGGCCCAGAGGGTGTGAGGTCAGGCTGGCGGCGGCGTCGTCGGCTGCGACCGGGGCCGGCGTCGCGCGTCC | ||
| >520999_FOXL2_16 | GCGGCGGCGTCGTCGGCTGCGACCGGGGCCGGCGTCGCGCGTCCCTGCATCCTCGCATCCGTCTGCACCGGCATGCGGTGGGCTCTCAGAGATCGAGGCGCGAATGCAGCGCGCCG | ||
| >520999_FOXL2_17 | GCGCGAATGCAGCGCGCCGGTCTTGCTGTCGTGGTCCCAGTAAGAGCAATGCATCATGGCGAGCTCGGGCTGCCGGGCACAAGCGAACTGCAGGCCCGGCGCACTGGTGGGCGCGGGCGCCGGGGGCGCGGCG | ||
| >520999_FOXL2_18 | CCGGGCACAAGCGAACTGCAGGCCCGGCGCACTGGTGGGCGCGGGCGCCGGGGGCGCGGCGGTGGCTGGGCTGGCAGGGCTGAGCTGGCCCGGCGGCGGCGCGGCGGCCCCGTGGTGCGGTGGGGCAGGCGGCGGTGCGGCGGCC | ||
| >520999_FOXL2_19 | GCGGCGGTGCGGCGGCCGCGTGCAGATGGTGTGCGTGCGGATGCGGGTGGGGGTGCGGCGGAGGCGGGGGTGCGGCCGGCGGGCCTCCCAGGCCATTGTACGAGTTCACTACGCCGGGGGGCAGCGC | ||
| >520999_FOXL2_20 | CCGGGGGGCAGCGCCATGCTCTGCACGCGTGTGTACGGCCCGTACGAGGCGGCCGGGCCCGCCAGCCCCTTGACCACAGCGGCCGCGCCAGGGCTACCGGGGCCCGCGG | ||
| >520999_FOXL2_21 | CGGCCGCGCCAGGGCTACCGGGGCCCGCGGCTGCAGCCGCAGCTGCTGCAGCCGCTGCGGCTGCCGCCATCTGGCAGGAGGCATAGGGCATGGGTGAGGGAGGCTGCGGTAGCGGCCACGAGTTGTT | ||
| >520999_FOXL2_22 | GGTGAGGGAGGCTGCGGTAGCGGCCACGAGTTGTTGAGGAAGCCAGACTGCAGGTACTTGGGGGGCGCCAGGTAGCCGTAGCCGTCGGCCCCGGCGCCCGCCACGCCGCACCCGCCTGCGGCGCCTCCGGCCCCGA | ||
| >520999_FOXL2_23 | GGGGGCGCCAGGTAGCCGTAGCCGTCGGCCCCGGCGCCCGCCACGCCGCACCCGCCTGCGGCGCCTCCGGCCCCGAAGAGCCCCTTGCCGGGCTGGAAGTGCGCGGGCGGCGGCCGGAAGGGCCTCTTCATGCGGCGGCGGCGC | ||
| >520999_FOXL2_24 | GGCGGCGGCGCCGGTAGTTGCCCTTCTCGAACATGTCTTCGCAGGCCGGGTCCAGCGTCCAGTAGTTGCCCTTGCGCTCGCCGCCGCCCTCGCGCGGCACCTTGATGAAGCACTCGTTGAG | ||
| >520999_FOXL2_25 | GCCGCCGCCCTCGCGCGGCACCTTGATGAAGCACTCGTTGAGGCTGAGGTTGTGGCGGATGCTATTTTGCCAGCCCTTCTTATTCTTCTCGTAGAACGGGAACTTCGCGATGATGTACTGGTAGATGCCG | ||
| >520999_FOXL2_26 | TAGATGCCGGACAGCGTGAGCCTCTTCTCCGCGCTCTCGCGGATCGCCATGGCGATGAGCGCCACGTACGAGTACGGGGGCTTCTGCGCCGG | ||
| >520999_FOXL2_27 | GGGGCTTCTGCGCCGGGTCCGGCTTCTCCGGGGCTGTCCCGCCGCCACCCCCACCGCCCTTGCCTGGGCTCGGCGGCGGCCCTTCTGGCTCCTTGACTGTGCGACCGGTCTCTGGGGCCAGCAGGGCCCCCGCCGCGTCCTCGG | ||
| >520999_FOXL2_28 | GGGCCAGCAGGGCCCCCGCCGCGTCCTCGGGCTCGGGGTAGCTGGCCATCATGACAAAGCCGGCGCGCCGCGGCCGGGCCGCCTCTGCTCTCCGCTCCAGGCGCTGGCGC | ||
| >520999_FOXL2_29 | GGCGCGCCGCGGCCGGGCCGCCTCTGCTCTCCGCTCCAGGCGCTGGCGCGGCAAAGAGTTGGGGCGCACGAGTCCGCTTACGGCCAAGTCTCAAACTTCTGGAGACTGCGGATGCCGCCCGCG | ||
| >520999_FOXL2_30 | CCGCCCGCGCTTGCTTGCTGGAGGCCTGTCGCTGCTCTCCCCTCTCCTTCCCCTTCCCCTAGGGAGCGGCCGGCGGGAGTGGAGCTCAGCCTCTGGCCATGGGGAGTCCGCC | ||
| >520999_FOXL2_31 | GGGAGTCCGCCCAACAGAGAGGGGCTCCGGCCTCGCCGCCCCTCCCCGCTCAGGCCAGTCCCCGCCTTGGTGGGTTTTCTTTTCTGCGCTCTTCCCCTCCCCCCGCCCCCCG | ||
| >520999_FOXL2_32 | CCTCCCCCCGCCCCCCGGTTTCCCGAAGCACGACCCGCGTCTCTGGCGGAGCTGCCTCCTGGAGTCCCTAGTGCGCCAGGAGCCTCGCTCTGTTCTGATTCGTATGGG | ||
| >520999_KIT_01 | TGACATATGGCCATTTCTGTTTTCCTGTAGCAAAACCAGAAATCCTGACTTACGACAGGCTCGTGAATGGCATGCTCCAATGTGTGGCAGCAGGATTCCCAGAGCCCACAATAGATTGGTATTTTT | ||
| >520999_KIT_02 | ATTGGTATTTTTGTCCAGGAACTGAGCAGAGGTGAGATGATTATTTTTGGCACTGCTTATAATGCAGAG | ||
| >520999_KIT_03 | TATTTATTTATTTTCCTAGAGTAAGCCAGGGCTTTTGTTTTCTTCCCTTTAGATGCTCTGCTTCTGTACTGCCAGTGGATGTGCAGACACTAAACTCATCTGGGCCACCGTTTGGAAA | ||
| >520999_KIT_04 | GGCCACCGTTTGGAAAGCTAGTGGTTCAGAGTTCTATAGATTCTAGTGCATTCAAGCACAATGGCACGGTTGAATGTAAGG | ||
| >520999_KIT_05 | AATGTAAGGCTTACAACGATGTGGGCAAGACTTCTGCCTATTTTAACTTTGCATTTAAAGGTAACAACAAAGGTATATTTCTTTTTAATCCAATTTAAGGGGATGTTTA | ||
| >520999_KIT_06 | TTGTGATTCCACATTTCTCTTCCATTGTAGAGCAAATCCATCCCCACACCCTGTTCACTCCTTTGCTGATT | ||
| >520999_KIT_07 | TCCTTTGCTGATTGGTTTCGTAATCGTAGCTGGCATGATGTGCATTATTGTGATGATTCTGACCTACAAATATTTACAGGTAACCATTTATTTGTTCT | ||
| >520999_KIT_08 | AGACAATAATTATTAAAAGGTGATCTATTTTTCCCTTTCTCCCCACAGAAACCCATGTATGAAGTACAGTGGAAGGTTGTTGAGGAGATAAATGGAAACAATTATGTTTA | ||
| >520999_KIT_09 | ATAAATGGAAACAATTATGTTTACATAGACCCAACACAACTTCCTTATGATCACAAATGGGAGTTTCCCAGAAACAGGCTGAGTTTTGGTCAGTATGAAACAGGGGCTTTCCATGTCACCTTTTT | ||
| >520999_KIT_10 | ACCAGCACCATCACCACTTACCTTGTTGTCTTCCTTCCTACAGGGAAAACCCTGGGTGCTGGAGCTTTCGGGAAGGTTGTTGAGGCAACTGCTTATGGCTTAATTAAGTCAGATGCGG | ||
| >520999_KIT_11 | CTTAATTAAGTCAGATGCGGCCATGACTGTCGCTGTAAAGATGCTCAAGCGTAAGTTCCTGTATGGTACTGCATGCGCTTGACATCAGTTT | ||
| >520999_KIT_12 | CCAGTTGTGCTTTTTGCTAAAATGCATGTTTCCAATTTTAGCGAGTGCCCATTTGACAGAACGGGAAGCCCTCATGTCTGAACTCAAAGTCCTGAGTTACCTTGGTAATCACATGAATATT | ||
| >520999_KIT_13 | TAATCACATGAATATTGTGAATCTACTTGGAGCCTGCACCATTGGAGGTAAAGCCGTGTCCAAGCTGCCTTTTATTGTCTGTCAGGTTATCAAAACATGACATTTTAATATGATTTT | ||
| >520999_KIT_14 | GGTATTTTTATGGGAGGCAGAATTAATCTATATATCTCACCTTCTTTCTAACCTTTTCTTATGTGCTTTTAGGGCCCACCCT | ||
| >520999_KIT_15 | TTTTAGGGCCCACCCTGGTCATTACAGAATATTGTTGCTATGGTGATCTTTTGAATTTTTTGAGAAGAAAACGTGATTCATTTATTTGTT | ||
| >520999_KIT_16 | ATTTATTTGTTCAAAGCAGGAAGATCATGCAGAAGCTGCACTTTATAAGAATCTTCTGCATTCAAAGGAGTCTTCCTGGTAAGACTGATTTACATAAATAGTTAGCTGTT | ||
| >520999_KIT_17 | TAATAGTGTATTCACAGAGACTTGGCAGCCAGAAATATCCTCCTTACTCATGGTCGGATCACAAAGATTTGTGATTTT | ||
| >520999_KIT_18 | ATTTGTGATTTTGGTCTAGCCAGAGACATCAAGAATGATTCTAATTATGTGGTTAAAGGAAACGTGAGTACCCATTCTCTGCTTGACAGTCCTGCAAAGGATTTTTA | ||
| >520999_KIT_19 | TATAAGAATATCTTCTGTTCAATTTTGTTGAGCTTCTGAATTAACATTATTGACTCTGTTGTGCTTCTATTACAGGCTCGACTACCTGTGAAGTGGATGGCACCTGAAAGCATTTTCAACTGTGTATACA | ||
| >520999_KIT_20 | CATTTTCAACTGTGTATACACGTTTGAAAGTGACGTCTGGTCCTATGGGATTTTTCTTTGGGAGCTGTTCTCTTTAGGTAAAATGATCCTTGCCAAAGACAACTTCATTA | ||
| >520999_KRAS_01 | ATCTGTATTTATTTCAGTGTTACTTACCTGTCTTGTCTTTGCTGATGTTTCAATAAAAGGAATTCCATAACTTCTTGCTAAGTCCTGA | ||
| >520999_KRAS_02 | AATAAAAGGAATTCCATAACTTCTTGCTAAGTCCTGAGCCTGTTTTGTGTCTACTGTTCTAGAAGGCAAATCACATTTATTTCCTACTA | ||
| >520999_KRAS_03 | ATTTATTTCCTACTAGGACCATAGGTACATCTTCAGAGTCCTTAACTCTTTTAATTTGTTCTCTGGGAAAGAAAAAAAAGTTATAGCACAGTCATTAGTAACACAAATAT | ||
| >520999_KRAS_04 | TTATTATATTCAATTTAAACCCACCTATAATGGTGAATATCTTCAAATGATTTAGTATTATTTATGGCAAATACACAAAGAAAGCCCTCCCCAGTCCTCAT | ||
| >520999_KRAS_05 | CAGTCCTCATGTACTGGTCCCTCATTGCACTGTACTCCTCTTGACCTGCTGTGTCGAGAATATCCAAGAGACAGGTTTCTCCATCAATTACTACTTGCTTCCTGTAGGAATCCTGAGAAGGGAGAAA | ||
| >520999_KRAS_06 | CAGTAATATGCATATTAAAACAAGATTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCA | ||
| >520999_KRAS_07 | TTGCCTACGCCACCAGCTCCAACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAATAATGAAAATGTGACTATATTAGAACATGTCACACATAAGGTTAATA | ||
| >520999_MET_01 | GTTCACTGCATATTCTCCCCACAGATAGAAGAGCCCAGCCAGTGTCCTGACTGTGTGGTGAGCGCCCTGGGAGCCAAAGTCCTTTCATCTGTAAAGGACCGGTTCATCAACTT | ||
| >520999_MET_02 | GGTTCATCAACTTCTTTGTAGGCAATACCATAAATTCTTCTTATTTCCCAGATCATCCATTGCATTCGATATCAGTGAGAAGGCTAAAGGAAACGAAAGATGGTTTTATGTTTTT | ||
| >520999_MET_03 | CTGAATGATGACATTCTTTTCGGGGTGTTCGCACAAAGCAAGCCAGATTCTGCCGAACCAATGGATCGATCTGCCATGTGTGCATTCCCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAACAAAAA | ||
| >520999_MET_04 | CCTATCAAATATGTCAACGACTTCTTCAACAAGATCGTCAACAAAAACAATGTGAGATGTCTCCAGCATTTTTACGGACCCAATCATGAGCACTGCTTTAATAGGGTAAGT | ||
| >520999_MET_05 | CTTTAATAGGGTAAGTCACATCAGTTCCCCACTTATAAACTGTGAGGTATAAATTAGAAATAAGTATCAGTCTCAAAAAGAATATCCAGGGCTTCTTTTGTG | ||
| >520999_MET_06 | TGAGGTATAAATTAGAAATAAGTATCAGTCTCAAAAAGAATATCCAGGGCTTCTTTTGTGCTTTGTAAATGGTGTTTATCCAAAATAGTTGCAGATTTTTTCCAAGAAAATT | ||
| >520999_MET_07 | ATTTTTTCCAAGAAAATTGAGGAATTGAATCTTCATTTACACCTAAAATTATATCTTTAAAATGTAAATGGTAACTAAAAGAAAAATGTTTTTACAATT | ||
| >520999_MET_08 | AGGTGATTAAATTGAATCCCTCTCTTACAGTACTTGGTGGAAAGAACCTCTCAACATTGTCAGTTTTCTATTTTGCTTTGCCAGTGGTGGGAGCACAATAA | ||
| >520999_MET_09 | ACAATAACAGGTGTTGGGAAAAACCTGAATTCAGTTAGTGTCCCGAGAATGGTCATAAATGTGCATGAAGCAGGAAGGAACTTTACAGTGGTAAGTCCTTTGAGCAATGGTTCTACT | ||
| >520999_MET_10 | TTATTATCCTGAAGGCAGTTATGCCATTTGTAGAATGGTAATAACCAGTTGGTATTTGGGACCCAAAGTGCTACAACCTGTGTAGTACAAATAT | ||
| >520999_MET_11 | TACAAATATCTATCATGGCTAAATGCTGACTTTTCTTTATTTGTCATTTTTAGTGGAAGCAAGCAATTTCTTCAA | ||
| >520999_MET_12 | TCTCCTGGGGCCCATGATAGCCGTCTTTAACAAGCTCTTTCTTTCTCTCTGTTTTAAGATCTGGGCAGTGAATTAGTTCGCTACGATGCAAGAGTACACACTCCTCATTTG | ||
| >520999_MET_13 | TACACACTCCTCATTTGGATAGGCTTGTAAGTGCCCGAAGTGTAAGCCCAACTACAGAAATGGTTTCAAATGAAT | ||
| >520999_MET_14 | AGAAATGGTTTCAAATGAATCTGTAGACTACCGAGCTACTTTTCCAGAAGGTATATTTCAGTTTATTGTTCTGAGAAATA | ||
| >520999_MET_15 | TAAATTTGACAAAAGTATTCACTGTTCCATAATGAAGTTAATGTCTCCACCACTGGATTTCTCAGGAATCACTGACATAGGAGAAGTTTCCCAATTT | ||
| >520999_MET_16 | ATAGGAGAAGTTTCCCAATTTCTGACCGAGGGAATCATCATGAAAGATTTTAGTCATCCCAATGTCCTCTCGCTCCTGGGAATCTGCCTGCGAAGTGAAGGGTCTCCGCT | ||
| >520999_MET_17 | GGTCTCCGCTGGTGGTCCTACCATACATGAAACATGGAGATCTTCGAAATTTCATTCGAAATGAGACTCATGTAAGTTGACTGCCAAGCTTACTAACTGGCAAACTA | ||
| >520999_MTHFR_01 | CCCGCAGCCTGGCCTGCAGCTGGGGTCAGGCCAGGGGCAGGGGATGAACCAGGGTCCCCACTCCAGCATCACTCACTTT | ||
| >520999_MTHFR_02 | ACTCACTTTGTGACCATTCCGGTTTGGTTCTCCCGAGAGGTAAAGAACGAAGACTTCAAAGACACTTTCTTCACTGGTCAGCTCCTCCCCCCACATCTTCAGCAGCTCCTCCTTGGGGGACTTGCTCTTCAGGTA | ||
| >520999_MTHFR_03 | GGACTTGCTCTTCAGGTAGAAGAGGTAGTAGTCCTTCAGCTCCCCAAAGGCAGGGGAAGAGGAATTGCCCCTGGCAGAGGGGTGCCCAGAGGTCAGGGCACACTCCTGACAGAGGGCAGTGCCAC | ||
| >520999_MTHFR_04 | CCCCCAGCCTGTGCGAGGACGGTGCGGTGAGAGTGGGGTGGAGGGAGCTTATGGGCTCTCCTGGGCCCCTCACCTGGATGGGAAAGATCCCGGGGACGATGGGGCAAGTGATGCCCATG | ||
| >520999_MTHFR_05 | GCCCCTCACCTGGATGGGAAAGATCCCGGGGACGATGGGGCAAGTGATGCCCATGTCGGTGCATGCCTTCACAAAGCGGAAGAATGTGTCAGCCTCAAAGAAAAGCTGCGTGATGATGAAATCGGCTCCC | ||
| >520999_MTHFR_06 | GTGATGATGAAATCGGCTCCCGCAGACACCTTCTCCTTCAAGTGCTTCAGGTCAGCCTCAAAGCTCCCTGCTTCGGGGTGGCCTTTGGGGTAAC | ||
| >520999_MTHFR_07 | CTCCCTGCTTCGGGGTGGCCTTTGGGGTAACCTGCCAATAGGGATGACAGTCAGGAGAGGCTGGCCTCCACC | ||
| >520999_NRAS_01 | ACAAATGCTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATGGAATCCCGTAACTCTTGGCCAGTTCGTGGGCTTGTTTTGTATCAACTGTCCTTGTTGG | ||
| >520999_NRAS_02 | TCCTTGTTGGCAAATCACACTTGTTTCCCACTAGCACCATAGGTACATCATCCGAGTCTTTTACTCGCTTAATCTGCTCCCTAAAAACGGGAATATATTAT | ||
| >520999_NRAS_03 | TTTCAGAGAAAATAATGCTCCTAGTACCTGTAGAGGTTAATATCCGCAAATGACTTGCTATTATTGATGGCAAATACACAGAGGAAGCCTTCGCCTGTCCTCATGTATTGGTCTCTCATG | ||
| >520999_NRAS_04 | TCCTCATGTATTGGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCCAACAAACAGGTTTCACCATCTATAA | ||
| >520999_NRAS_05 | CCATCTATAACCACTTGTTTTCTGTAAGAATCCTGGGGGTGTGGAGGGTAAGGGGGCAGGGAGGGAGGGAAGTTCAATTTTTATTAAAAACCACAGGGAATG | ||
| >520999_NRAS_06 | ATCAGGTCAGCGGGCTACCACTGGGCCTCACCTCTATGGTGGGATCATATTCATCTACAAAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACCTGCTCCAA | ||
| >520999_NRAS_07 | CACCTGCTCCAACCACCACCAGTTTGTACTCAGTCATTTCACACCAGCAAGAACCTGTTGGAAACCAGTAATCAGGGTTAATTGGC | ||
| >520999_PDGFRA_01 | CTTTGGTAATTCACCAGTTACCTGTCCTGGTCATTTATAGAAACCGAGGTATGAAATTCGCTGGAGGGTCATTGAATCAATCAGCCCAGATGGACATGAATATATTTATGT | ||
| >520999_PDGFRA_02 | CAGATGGACATGAATATATTTATGTGGACCCGATGCAGCTGCCTTATGACTCAAGATGGGAGTTTCCAAGAGATGGACTAGTGCTTGGTAAGTTCCATGGGGTAACCTCCCAAGACTCCCTTTT | ||
| >520999_PDGFRA_03 | GGACTGATATGTGATTTATTCTTTCAACAGCCACGGCCAGATCCAGTGAAAAACAAGCTCTCATGTCTGAAC | ||
| >520999_PDGFRA_04 | TCATGTCTGAACTGAAGATAATGACTCACCTGGGGCCACATTTGAACATTGTAAACTTGCTGGGAGCCTGCACCAAGTCAGGTGGGCTCACTGACCTGGAGTGAGGATTTTCACTGGACACATGT | ||
| >520999_PDGFRA_05 | ATTTCTTCCTTTTCCATGCAGTGTGTCCACCGTGATCTGGCTGCTCGCAACGTCCTCCTGGCACAAGGAAAAATTGTGAAGATCTGTGACTTTGGCCTGGCCAGAGACATCATGCAT | ||
| >520999_PDGFRA_06 | CAGAGACATCATGCATGATTCGAACTATGTGTCGAAAGGCAGTGTACGTCCTCACTTCCCTCACTGGTCAGGCTCATCCTCCTTCACTTTAATCTCTAAA | ||
| >520999_PIK3CA_01 | TATATAATAGCTTTTCTTCCATCTCTTAGGAAACTCCATGCTTAGAGTTGGAGTTTGACTGGTTCAGCAGTGTGGTAAAGTTCCCAGATATGTCAGTGATTGAAGAGCATGCCAATT | ||
| >520999_PIK3CA_02 | TGATTGAAGAGCATGCCAATTGGTCTGTATCCCGAGAAGCAGGATTTAGCTATTCCCACGCAGGACTGGTAAGGCAAATCACTGAGTTTATTAAGTATCAATTATAATCTGT | ||
| >520999_PIK3CA_03 | TTTTCTCTGTTTAAAATGTTTTGGTGTTCTTAATTTATTCAAGACATTTTGTATCTGCATATATCAAACTATAACATAATTTCTTATTTTTGAAAGCTGTTT | ||
| >520999_PIK3CA_04 | TATAACATAATTTCTTATTTTTGAAAGCTGTTTCATATAGATTTTGGACACTTTTTGGATCACAAGAAGAAAAAATTTGGTTATAAACGAGAACGTGTGCCATTTGTTTTGACACA | ||
| >520999_PIK3CA_05 | TTTTGACACAGGATTTCTTAATAGTGATTAGTAAAGGAGCCCAAGAATGCACAAAGACAAGAGAATTTGAGAGGTGAGCTCGAGCAATTAAAAACACAAAATAAAGA | ||
| >520999_RET_01 | CTAGAGAGTTAGAGTAACTTCAATGTCTTTATTCCATCTTCTCTTTAGGGTCGGATTCCAGTTAAATGGATGGCAATTGAATCCCTTTTTGATCATATCTACA | ||
| >520999_RET_02 | TTTTTGATCATATCTACACCACGCAAAGTGATGTGTAAGTGTGGGTGTTGCTCTCTTGGGGTGGAGGTTACAGAAACA | ||
| >520999_TP53_01 | GGTTCAAAGACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTGTCAGTGGGGAACAAGAAGTGGAGAATGTCAGTCTGAGTCAGGCCCTTCTGTCTTGAACATGA | ||
| >520999_TP53_02 | TCTGAGTCAGGCCCTTCTGTCTTGAACATGAGTTTTTTATGGCGGGAGGTAGACTGACCCTTTTTGGACTTCAGGTGGCTGTAGGAGACAGAAGCAGGGAGGAGAGATG | ||
| >520999_TP53_03 | GGATGAGAATGGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAAGGGGCTGAGGTCACTCACCTGGAGTGAGCCCTGCTCCCCCCTGGCTCCTTCCCAGCCTGG | ||
| >520999_TP53_04 | CCCTGCTCCCCCCTGGCTCCTTCCCAGCCTGGGCATCCTTGAGTTCCAAGGCCTCATTCAGCTCTCGGAACATCTCGAAGCGCTCACGCCCA | ||
| >520999_TP53_05 | TTCAGCTCTCGGAACATCTCGAAGCGCTCACGCCCACGGATCTGCAGCAACAGAGGAGGGGGAGAAGTAAGTATATACACAGTACCTGAGTTAAAAGATGGTTCAAGTTACAATTGTTT | ||
| >520999_TP53_06 | TTAGATTAGGTGTATTAAATCCATTTTCAACTTACAATATTTTCAACTTACGACGAGTTTATCAGGAAGTAACACCATCGTAAGTCAAGTAGCATCTGTAT | ||
| >520999_TP53_07 | TAGCATCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATTTTCTTTTTGAAAGCTGGTCTGGTCCTTTAAAATATATATTATGGTAT | ||
| >520999_TP53_08 | AACTTTCCACTTGATAAGAGGTCCCAAGACTTAGTACCTGAAGGGTGAAATATTCTCCATCCAGTGGTTTCTTCTTTGGCTGGGGAGAGGAGCTG | ||
| >520999_TP53_09 | GGGAGAGGAGCTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAGTGAATCTGAGGCATAACTGCACCCTTGGTCTCCTCCACCGCTTCTTGT | ||
| >520999_TP53_10 | CGCTTCTTGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTGAGGCTCCCCTTTCTTGCGGAGATTCTCTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGC | ||
| >520999_TP53_11 | TGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCACCTCAAAGCTGTTCCGTCCCAGTAGATTACCACTACTCAGGATAGGAAAAGAGAAGCAA | ||
| >520999_TP53_12 | AAGAAATCGGTAAGAGGTGGGCCCAGGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGTGGCAAGTGGCTCCTGACCTGGAGTC | ||
| >520999_TP53_13 | CTGACCTGGAGTCTTCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCAGGAACTGTTACACATGTAGTTGTAGTGGATGGTG | ||
| >520999_TP53_14 | TTACACATGTAGTTGTAGTGGATGGTGGTACAGTCAGAGCCAACCTAGGAGATAACACAGGCCCAAGATGAGGCCAGTGCG | ||
| >520999_TP53_15 | CCCAGAGACCCCAGTTGCAAACCAGACCTCAGGCGGCTCATAGGGCACCACCACACTATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTC | ||
| >520999_TP53_16 | AATTTCCTTCCACTCGGATAAGATGCTGAGGAGGGGCCAGACCTAAGAGCAATCAGTGAGGAATCAGAGGCCTGGGGACCCTGGGCAACCAGCCCTGTCGTCTCTCCAGCCC | ||
| >520999_TP53_17 | GTCTCTCCAGCCCCAGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCTCACAACCTCCGTCATGTGCTGTGA | ||
| >520999_TP53_18 | ATGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGCGGGGGTGTGGAATCAACCCACAGCTGCACAGGGCAGGTCTTGGCCAGTT | ||
| >520999_TP53_19 | GGGCAGGTCTTGGCCAGTTGGCAAAACATCTTGTTGAGGGCAGGGGAGTACTGTAGGAAGAGGAAGGAGACAGAGTTGAAAGTCAGGGCACAAGTGAA | ||
| >520999_TP53_20 | ATGGAAGCCAGCCCCTCAGGGCAACTGACCGTGCAAGTCACAGACTTGGCTGTCCCAGAATGCAAGAAGCCCAGACGGAAACCGTAGCTGCC | ||
| >520999_TP53_21 | CGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGACAGGGGCCAGGAGGGGGCTGGTGCAGGGGCCGCCGGTGTAGGAGCTGCTGGTGCAGGGGCCACGGGGGGAGCAG | ||
| >520999_TP53_22 | GGCCACGGGGGGAGCAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAACCATTGTTCAATATCGTCCGGGGACAGCATCAAAT | ||
| >520999_TP53_23 | GGGGACAGCATCAAATCATCCATTGCTTGGGACGGCAAGGGGGACT | ||
| >520999_TP53_24 | GGCAAGGGGGACTGTAGATGGGTGAAAAGAGCAGTCAGAGGACCAGGTCCTCAGCCCCCCAGCCCCCCAGCCCTCCAGGTCCCCAGCCCTCCAGGTCCCCAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAG | ||
| >520999_TP53_25 | TTGTTTTCAGGAAGTCTGAAAGACAAGAGCAGAAAGTCAGTCCCATGGAATTTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTG | ||
| >520999_TP53_26 | AATTTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTGGTGGGCCTGCCCTTCCAATGGATCCACTCACAGTTTCCATAGGTCTGAAAATGTTTC | ||
| >520999_TP53_27 | AAAATGTTTCCTGACTCAGAGGGGGCTCGACGCTAGGATCTGACTGCGGCTCCTCCATGGCAGTGACCCGGAAGGCAGTCTGG | ||
| >520999_TPMT_01 | 121 | ATTTTATTTTATCTATGTCTCATTTACTTTTCTGTAAGTAGATATAACTTTTCAAAAAGACAGTCAATTCCCCAACTTTTATGTCGTTCTTCAAAA | |
| >520999_TPMT_02 | 122 | TTTTATGTCGTTCTTCAAAAGCATCAACCTTCTCAAGACAACGTATATTGCATATTTTACCTGAAACAAGAAAGAGTAACATGTTAAAATA | |
| >520999_TPMT_03 | 123 | AAAAAAAAAAAACCCAACAACTTTACCTGGATGTTTAGTTGGATCATAAGAAAGAACACACAGGAGATACTGAAACTTCTTTCCCAGGAGGGAAAACATT | |
| >520999_TPMT_04 | 124 | TGAAACTTCTTTCCCAGGAGGGAAAACATTGTATCTGCATAGCTACAAAGAACACAAGAAGGTATTTGTTACATTTCTCTACACAGGCAAAGGCTGGAGGGACAAAAGGCAG | |
| >520999_TPMT_05 | 125 | AAAAAGTATAGTATACTAAAAAATTAAGACAGCTAAACAAAAAAAGAAAAATTACTTACCATTTGCGATCACCTGGATTGATGGCAACTAATGCT | |
| >520999_TPMT_06 | 126 | ATTGATGGCAACTAATGCTCCTCTATCCCAAATCATGTCAAATTTGCCAATATTTGTCCTACCAGAAAGAGAAAAAACATTTTATGGGAGAAAAATCAAATCTTTAAGAAGATGAGCAGCGTCCCC | |
| >520999_TPMT_07 | 127 | AATTATTTACCCAAATCAAAACAAACCTTAAATACTTTGGTTCCAGGAATTTCGGTGATTGGTTCTTCTGAGTAAGAAAGATTCTGCTCTGTAAAAAATTCTTGTATC | |
| >520999_TPMT_08 | 128 | TAAAAAATTCTTGTATCCCAAGTTCACTGATTTCCACACCAACTACACTGTGTCCCCGGTCTGCAAACCTGCATAAAATCATACATTTACACTTAAATTATGTTTTCAAAT | |
| >520999_UGT1A1_01 | TATTAAGAAACCTAATAAAGCTCCACCTTCTTTATCTCTGAAAGTGAACTCCCTGCTACCTTTGTGGACTGACAGCTTTTTATA | ||
| >520999_UGT1A1_02 | AGCTTTTTATAGTCACGTGACACAGTCAAACATTAACTTGGTGTATCGATTGGTTTTTGCCATATATATATATATAAGTAGGAGAGGGC | ||
| >520999_UGT1A1_03 | ATTGGTTTTTGCCATATATATATATATAAGTAGGAGAGGGCGAACCTCTGGCAGGAGCAAAGGCGCCATGGCTGTGGAGTCCCAGGGCGGACGCCCACTTGTCCTGGGCCTGCTGCTGTGTGTGCTGGGCC | ||
| >520999_UGT1A1_04 | CCAGGGCGGACGCCCACTTGTCCTGGGCCTGCTGCTGTGTGTGCTGGGCCCAGTGGTGTCCCATGCTGGGAAGATACTGTTGATCCCAGTGGATGGCAGCCACTGGCTGAGCATGCTTGGGGCCATCCAGCA | ||
| >520999_UGT1A1_05 | GGCCATCCAGCAGCTGCAGCAGAGGGGACATGAAATAGTTGTCCTAGCACCTGACGCCTCGTTGTACATCAGAGACGGAGCATTTTACA | ||
| >520999_UGT1A1_06 | ACGGAGCATTTTACACCTTGAAGACGTACCCTGTGCCATTCCAAAGGGAGGATGTGAAAGAGTCTTTTGTTAGTCTCGGGCATAATGTTTTTGAGAATGATTCTTT | ||
| >520999_UGT1A1_07 | TTTTTGAGAATGATTCTTTCCTGCAGCGTGTGATCAAAACATACAAGAAAATAAAAAAGGACTCTGCTATGCTTTTGTCTGGCTGTTCCCACTTACTGCACAACAAGGAGCTCATGGCCTCC | ||
| >520999_UGT1A1_08 | TCATGGCCTCCCTGGCAGAAAGCAGCTTTGATGTCATGCTGACGGACCCTTTCCTTCCTTGCAGCCCCATCGTGGCCCAGTACCTGTCTCTGCCCACTGTATTCTTCTTGCATGCACTGCCATGCA | ||
| >520999_UGT1A1_09 | ACTGCCATGCAGCCTGGAATTTGAGGCTACCCAGTGCCCCAACCCATTCTCCTACGTGCCCAGGCCTCTCTCCTCTCATTCAGATCACATGACCTTCCTGCAGCGGGTGAAGAA | ||
| >520999_UGT1A1_10 | GGGTGAAGAACATGCTCATTGCCTTTTCACAGAACTTTCTGTGCGACGTGGTTTATTCCCCGTATGCAACCCTTGCCTCAGAATTCCTTCAGAGAGAGGTGACTGTCCAGGA | ||
| >520999_UGT1A1_11 | TTCAGAGAGAGGTGACTGTCCAGGACCTATTGAGCTCTGCATCTGTCTGGCTGTTTAGAAGTGACTTTGTGAAGGATTACCCTAGGCCCATCATGCCCAATATGGTTTTTGTT | ||
| >520999_UGT1A1_12 | CCAATATGGTTTTTGTTGGTGGAATCAACTGCCTTCACCAAAATCCACTATCCCAGGTGTGTATTGGAGTGGGACTTTTACATGCGTATATTCTTTCAGATGTATTACTTT | ||
본 발명에 있어서, 상기 방법은 상기 증폭된 산물을 정제하는 단계를 추가로 포함할 수 있으며, 상기 정제는 당분야에서 통상적으로 사용하는 방법으로 수행될 수 있으며, 바람직하게는 비드를 사용하여 정제할 수 있다.
또한 본 발명은 암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 상기 프라이머를 포함하는 것을 특징으로 하는, 시료의 DNA로부터 표적 부위를 증폭하기 위한 조성물을 제공한다.
상기 조성물은 상기 프라이머로 DNA를 증폭하는데 통상적으로 사용하는 성분들을 추가로 포함할 수 있다.
본 발명에 있어서, 상기 암 유전자 또는 항암제 대사관련 유전자는 상기 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 표적 부위는 상기 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것일 수 있다.
본 발명에 있어서, 상기 프라이머는 상기 표 2에 기재된 서열번호 1 내지 96의 프라이머로부터 선택되는 것일 수 있다.
본 발명은 또한 상기 조성물, 어댑터 및 바코드 프라이머를 포함하는 것을 특징으로 하는, 암 유전자 및 항암제 대사 관련 유전자 변이 진단 패널을 제공한다.
상기 어댑터는 바코드와 결합하는 기능을 하며, 10 내지 20개의 임의의 핵산으로 이루어질 수 있다.
상기 바코드 프라이머는 분석 대상 DNA의 단일 가닥 DNA 파편에 바코드를 부여하는 역할을 수행하며, 4개 내지 30개의 임의의 핵산으로 이루어질 수 있다.
본 발명에 있어서, 상기 진단 패널은, 상기 어댑터의 부착에 필요한 성분 및 바코드 프라이머로 서열을 증폭하는데 필요한 성분들을 추가로 포함할 수 있다.
본 발명에 있어서, 상기 최종 산물의 서열을 분석하여 상기 표적 부위에 대한 앰플리콘의 서열과 비교하여 유전자 변이를 검출하는 것은 통상의 차세대 염기서열 분석법에서 사용하는 방법으로 수행될 수 있다.
예를 들어, 대상 시료의 염기서열 데이터(sequence reads)를 레퍼런스 염기서열(reference Genome)과 비교하는 매핑(Mapping)을 통해 대상 시료와 레퍼런스 염기서열의 차이를 알아낸 후 이를 적당한 선택 기준을 정해 신뢰할 수 있는 염기서열 변이 정보만 추출(Variant Calling)하게 된다. 그런 다음 염기서열 변이 정보를 기존 데이터베이스(dbSNP)와 비교하여 이미 밝혀진 변이인지 새롭게 발견된 변이인지 판단한다. 그리고 그 변이가 아미노산의 변화를 가져올 것인지 아닌지, 또한 단백질 구조에 있어서 어떤 영향을 줄 것인지 예측하게 된다. 이 과정을 주석달기(Annotation)라고 한다. 추출한 변이에 관한 정보는 정보의 품질을 더 높이기 위하여 데이터베이스에 등재하거나 전장유전체연관분석 (Genome Wild Association Study; GWAS)과 통합 연구를 통해 질병의 원인 변이를 찾는 연구를 수행할 수도 있다.
본 발명의 암 세포의 유전체 DNA의 변이 및 약물 반응 유전자의 변이를 검출 및 진단하기 위한 방법은 주요 암 관련 유전자 및 약물 대사유전자의 다양한 유전자 변이를 단 한번의 실험을 통해 검출할 수 있어 각각의 유전체 변이마다 다른 플랫폼을 사용하는 종래 방법보다 매우 경제적이고 효율적이다. 또한 암의 유발, 진행 및 약물 대사 관련된 주요 유전자를 대상으로 높은 커버리지 (high coverage)로 시퀀싱함으로써 고해상도를 확보하여 기존 방법으로 검출이 되지 않았던 낮은 빈도 유전체 변이의 동정이 가능하다. 따라서 본 발명의 방법을 이용하면 암 세포의 유전체 DNA의 변이와 약물 반응을 예측하는 유전자 변이를 포함하는 시료에서 다양한 유전자 변이를 동시에 높은 민감도와 정확도로 분석할 수 있고, 이러한 분석 결과를 바탕으로 환자 맞춤형 암 치료제를 효율적으로 탐색할 수 있다.
이하 본 발명을 실시예에 의하여 더욱 상세하게 설명한다. 하기 실시예는 단지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들 실시예에 국한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
<실시예 1>
프라이머 및 앰플리콘의 제조
상기 표 1의 한국인 호발 암 유전자를 증폭하기 위한 정방향 및 역방향 프라이머 및 앰플리콘을 (Bioo Scientific.USA )에 의뢰하여 제작하였으며 , 각 유전자에 대한 프라이머명 및 프라이머 서열, 및 앰플리콘명 및 앰플리콘 서열을 상기 표 2 및 3에 각각 나타내었다.
<실시예 2>
시료로부터 변이 검출
도 1에 도시된 바와 같은 단계로 개발된 패널을 사용하여 변이가 알려진 암 세포주 DNA 및 FFPE DNA 로부터 유전자의 변이를 검출하였다.
단계 1: 타겟 유전자 증폭
HCT15, HCT116, HT29, A549, SW480, LA174T, MCF7, PC3 암 세포주 DNA 샘플 과 4개의 FFPE DNA의 돌연변이를 분석하기 위해 각각 2개의 파트로 나누고 마스터 믹스 (master mix)를 만들어 실험을 진행하였다.
1) 반응 1 내지 4
뉴클레아제가 없는 물 (nuclease free water) 20.4 μL에 10 내지 50 ng의 FFPE (Formalin-fixed, paraffin-embedded) DNA가 들어갈 수 있도록 농도를 맞추었다. 구체적으로, 상기 반응 1 내지 4 시약의 조성은 하기 표 4와 같으며, 각 샘플을 제조하여 96-웰 PCR 플레이트의 각 웰에 각각 분주하였다.
| 성분 | 부피 |
| FFPE DNA 10-50 ng/20.4μL | |
| Nuclease-free Water | 20.4μL |
| NEXTflex® BRCA Plus Amplicon FFPE Primer Mix 1, 2, 3 또는 4 | 2.4 μL |
| NEXTflex® Hot Start PCR I Master Mix | 7.2 μL |
| 총부피 | 30 μL |
상기와 같은 조성의 시약을 피펫으로 충분히 혼합한 후, 유전자증폭기 (thermocycler)를 이용하여 하기 표 5와 같은 PCR 조건으로 PCR을 수행하였다.
| 1) 98℃, 2분 | 1 사이클 |
| 2) 95℃, 20초 | 21 사이클 |
| 3) 62℃, 4분 | |
| 4) 4℃ | 유지 |
2) 반응 5 내지 8:
뉴클레아제가 없는 물 15.6 μL에 10 내지 50 ng의 FFPE DNA가 들어갈 수 있도록 농도를 맞추었다. 구체적으로, 상기 반응 5 내지 8 시약의 조성은 하기 표 6와 같으며, 각 샘플을 제조하여 96-웰 PCR 플레이트의 각 웰에 각각 분주하였다.
| 성분 | 부피 |
| FFPE DNA 10-50 ng/15.6μL | |
| Nuclease-free Water | 15.6μL |
| NEXTflex® BRCA Plus Amplicon FFPE Primer Mix 5, 6, 7 또는 8 | 2.4 μL |
| NEXTflex® PCR Enhancer 1 | 0.6 μL |
| NEXTflex® PCR Enhancer 2 | 1.8 μL |
| NEXTflex® Hot Start PCR I Master Mix | 7.2 μL |
| 총부피 | 30 μL |
상기와 같은 조성의 시약을 피펫으로 충분히 혼합한 후, 유전자증폭기를 이용하여 하기 표 7과 같은 PCR 조건으로 PCR을 수행하였다.
| 1) 98℃, 2분 | 1 사이클 |
| 2) 98℃, 30초 | 2 사이클 |
| 3) 70℃, 4분 | |
| 4) 98℃, 30초 | 4 사이클 |
| 5) 68℃, 4분 | |
| 6) 98℃, 30초 | 4 사이클 |
| 7) 66℃, 4분 | |
| 8) 98℃, 30초 | 4 사이클 |
| 9) 64℃, 4분 | |
| 10) 98℃, 30초 | 8 사이클 |
| 11) 62℃, 4분 | |
| 12) 4℃ | 유지 |
단계 2: PCR 산물 정제
반응을 정지시키기 위해, 상기 단계 1에서 얻은 총 8개의 산물에 NEXTflex® Stop Solution 1 μL를 각각 분주하고 30초간 반응시켰다. 상기 반은 1 내지 4의 산물을 한 웰에 혼합하여 이를 세트 1로 하고, 상기 반응 5 내지 8의 산물을 한 웰에 혼합하여 이를 세트 2로 하였다.
세트 1로부터 16 μL 및 세트 2로부터 64 μL를 각각 추출하여 새로운 웰에 혼합하고, 48μL의 NEXTflex® Cleanup Beads를 추가하여 혼합한 다음, 상온에서 마그네틱 스탠드 상에서 5분간 반응시켰으며, 상층액이 육안으로 보았을 때 충분히 분리 되지 않았다면, 상층액이 투명해 질 때까지 반응시켰다. 투명한 상층액을 새로운 웰에 옮기고 72 μL의 NEXTflex® Cleanup Beads를 추가하고 다시 혼합하고 반응시키는 것을 반복하여 투명한 상층액을 얻었다. 상층액을 제거하고 남아있는 비드에 80% 에탄올 200μL를 천천히 분주하고 상온에서 30초간 반응시키는 과정을 2번 반복하고 에탄올을 완전히 제거하였다.
여기에 재현탁 완충액 30 μL를 첨가하여 혼합하고 상온에서 3분간 반응시킨 다음, 마그네틱 스탠드 상에서 5분간 반응시켜 상층액을 완전히 분리하였다. 분리된 상층액 28μL를 새로운 웰에 옮기고 이 용액을 정제된 PCR I 반응 (purified PCR ⅠRecation)으로 명명하였다.
단계 3: 어댑터 부착 (Adapter ligation)
상기 단계 2에서 얻은 반응물을 하기 표 8과 같은 성분 및 조성으로 얼음 위에서 혼합하였으며, 뉴클레아제가 없는 96-웰 PCR 플레이트를 사용하였다.
| 성분 | 부피 |
| 정제된 PCR I 반응 | 28 μL |
| NEXTflex® Amplicon DNA Adapter | 2.5 μL |
| NEXTflex® Ligation Mix | 42 μL |
| 총부피 | 72.5 μL |
상기 PCR plate를 완전히 밀봉한 후 유전자증폭기에서 22℃, 15분간 반응시켰다.
단계 4: 어댑터 부착 산물의 정제
상기 단계 3의 혼합물에 NEXTflex® Cleanup Beads 58μL를 분주하고 혼합한 다음, 상온에서 5분 반응시키고, 마그네틱 스탠드 위에서 상온에서 5분간 더 반응시켜 투명한 상층액을 얻었다.
이후, 상기 단계 2에서와 같이 비드를 에탄올로 세척하고 세척된 비드에 재현탁 완충액을 첨가하고 반응시켜 투명한 상층액을 얻었다 (정제된 어댑터 부착 DNA). 다음 단계에 사용하지 않은 샘플은 -20℃에 보관하였다.
단계 5: 최종 산물 증폭
상기 단계 4에서 얻은 상층액 38 μL를 새로운 웰에 분주하고 바코드 프라이머를 포함하는 하기 표 9와 같은 성분 및 조성으로 얼음 위에서 혼합하였으며, 뉴클레아제가 없는 96-웰 PCR 플레이트를 사용하였다.
| 성분 | 부피 |
| 정제된 어댑터 부착 DNA | 38 μL |
| NEXTflex® PCR II Barcoded Primer Mix | 2 μL |
| NEXTflex® PCR II Master Mix | 10 μL |
| 총부피 | 50 μL |
상기 PCR 플레이트를 밀봉한 후, 유전자증폭기를 이용하여 하기 표 10와 같은 PCR 조건으로 PCR을 수행하였다.
| (1) 65℃, 20분 | 1 사이클 |
| (2) 98℃, 2분 | 1 사이클 |
| (3) 98℃, 30초 | 14 사이클 |
| (4) 65℃, 30초 | |
| (5) 72℃, 60초 | |
| (6) 72℃, 4분 | 1 사이클 |
| (7) 4℃ | 유지 |
PCR이 완료된 후, 비드를 넣고 상기 단계 2에서와 같이 정제 과정을 수행하여 최종 산물을 얻었다.
단계 6: 최종 산물의 농도 측정 및 Miseq NGS 장비 구동
최종산물의 농도를 바이오어날라이져를 사용하여 측정하였으며, 최소 농도가 20 ng/ul 이상인 경우, Miseq NGS 장비를 이용하여 차세대 염기서열 분석 (NGS)을 수행하였고, 최소 농도가 20 ng/ul 이하인 경우, 상기 단계 1 내지 5를 반복하였다.
Miseq NGS 장비는 Miseq NGS 장비 매뉴얼에 따라 작동하였으며, 그 결과를 minifest파일에 따라 분석하고 그 결과를 Bam파일과 Vcf 파일로 얻었다 (파일명: NOVA-520999_ BRCA1-2_TP53_ Plus_FFPE_Manifest_v1.txt(도2)
<실시예 3>
NGS 호발암 패널 분석 결과의 데이터 분석
상기 실시예 2에서 얻은 데이터를 상용화된 데이터 분석 소프트웨어인 Illumina VariantStudio를 활용해서 분석하였다.
구체적으로, Illumina VariantStudio에 접속해서 상기 실시예 2에서 얻은 VCF 파일을 업로드한 후, variant pass filter로 제거한 다음, Qulity:99, Read Death: Cancer Gene 4000 이상 및 약물유전자 2000 이상으로 설정하고(도 3), 유전자 annotation & classification을 통해 정보를 링크하여 유전자 돌연변이 정보 확인하였다(도 4).
그 결과, A549 폐암 세포주에서 KRAS NM_033360.2:c.34G>A, NP_203524.1:p.Gly12Ser 돌연변이가 정확히 검출되는 것을 확인하였다.
<실시예 4>
NGS 데이터 비교
상기 실시예 3에서 얻은 데이터를 상용화되고 있는 Illumina Tumour 15와 Sanger sequencing을 통해 얻어진 데이터와 비교하여 개발 패널의 퍼포먼스를 확인 하였으며, 그 결과를 표 11에 나타내었다.
| Test Sample | AKT1 | BRAF | EGFR | ERBB2 | FOXL2 | KIT | |
| 개발 panal | HCT15 | ND | ND | ND | ND | ND | ND |
| HCT116 | ND | ND | ND | ND | ND | ND | |
| H1975 | ND | ND | T790M hetro L858R hetro | ND | ND | ND | |
| HT29 | ND | V600E, hetero | ND | ND | ND | ND | |
| A549 | ND | ND | ND | ND | ND | ND | |
| SW480 | ND | ND | ND | ND | ND | ND | |
| lIlumina tumor 15 panal & Sanger Sequencingl | HCT15 | ND | ND | ND | ND | ND | ND |
| HCT116 | ND | ND | ND | ND | ND | ND | |
| H1975 | ND | ND | T790M hetro L858R hetro | ND | ND | ND | |
| HT29 | ND | V600E, hetro | ND | ND | ND | ND | |
| A549 | ND | ND | ND | ND | ND | ND | |
| SW480 | ND | ND | ND | ND | ND | ND | |
| Test Sample | KRAS | MET | NRAS | PDGFRA | PIK3CA | RET | |
| 개발 panal | HCT15 | G13D hetro | ND | ND | ND | ND | ND |
| HCT116 | G13D homo | ND | ND | ND | ND | ND | |
| H1975 | ND | ND | ND | P567S hetero | ND | ND | |
| HT29 | ND | ND | ND | ND | ND | ND | |
| A549 | G12S homo | ND | ND | ND | ND | ND | |
| SW480 | G12V homo | ND | ND | ND | ND | ND | |
| lIlumina tumor 15 panal &Sanger Sequencingl | HCT15 | G13D hetro | ND | ND | ND | ND | ND |
| HCT116 | G13D homo | ND | ND | ND | ND | ND | |
| H1975 | ND | ND | ND | P567S hetero | ND | ND | |
| HT29 | ND | ND | ND | ND | ND | ND | |
| A549 | G12S homo | ND | ND | ND | ND | ND | |
| SW480 | G12V homo | ND | ND | ND | ND | ND | |
| Test Sample | TP53 | UGT1A1 | CYP2D6 | TPMT | MTHFR | DPYD | |
| 개발 panal | HCT15 | S241F | *1/*1 | *1/*1 | *1/*1 | 1298A/C | V815E |
| HCT116 | ND | *1/*1 | *1/*10B | *1/*1 | 677C/T | *1/*1 | |
| H1975 | ND | *1/*1 | *1/*11B | *1/*1 | ND | *1/1 | |
| HT29 | R273H | *1/*1 | *1/*1 | *1/*1 | 677C/T 1298A/C | *1/*9 | |
| A549 | ND | *1/*1 | *10B/*10B | *1/*1 | 1298C/C | *1/*1 | |
| SW480 | R273H P309S | *1/*1 | *1/*1 | *1/*1 | ND | *1/1 | |
| lIlumina tumor 15 panal & Sanger Sequencingl | HCT15 | S241F | *1/*1 | *1/*1 | *1/*1 | 1298A/C | V815E |
| HCT116 | ND | *1/*1 | *1/*10B | *1/*1 | 677C/T | *1/*1 | |
| H1975 | ND | *1/*1 | *1/*10B | *1/*1 | ND | *1/1 | |
| HT29 | R273H | *1/*1 | *1/*1 | *1/*1 | 677C/T 1298A/C | *1/*9 | |
| A549 | ND | *1/*1 | *10B/*10B | *1/*1 | 1298C/C | *1/*1 | |
| SW480 | R273H P309S | *1/*1 | *1/*1 | *1/*1 | ND | *1/1 |
그 결과, 정확도 100 %로 일치함을 알 수 있다.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.
<110> SPMED Co.,Ltd.
<120> NEXT GENERATION SEQUENCING (NGS)-BASED HYBRID DIAGNOSTIC PANEL
FOR ANALYZING VARIATION OF CANCER GENE AND ANTICANCER
DRUG-RELATED GENE
<130> DPP170184KR
<160> 128
<170> KoPatentIn 3.0
<210> 1
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_01_FOR
<400> 1
gggcacagga tggggaagtc acagc 25
<210> 2
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_01_REV
<400> 2
cgggagaacc aaaccggaat ggtcac 26
<210> 3
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_02_FOR
<400> 3
gaaccagggt ccccactcca gcatc 25
<210> 4
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_02_REV
<400> 4
tggggagctg aaggactact acctcttc 28
<210> 5
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_03_FOR
<400> 5
acatcttcag cagctcctcc ttggg 25
<210> 6
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_03_REV
<400> 6
tacaggaatg gcctcctggg catgtg 26
<210> 7
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_04_FOR
<400> 7
tcagcgaact cagcactcca cccagag 27
<210> 8
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_04_REV
<400> 8
cttccgcttt gtgaaggcat gcaccga 27
<210> 9
<211> 24
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_05_FOR
<400> 9
gagggagctt atgggctctc ctgg 24
<210> 10
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_05_REV
<400> 10
ctgaagcact tgaaggagaa ggtgtctgc 29
<210> 11
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_06_FOR
<400> 11
atgtgtcagc ctcaaagaaa agctgc 26
<210> 12
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_06_REV
<400> 12
ctctcctgac tgtcatccct attggcag 28
<210> 13
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_07_FOR
<400> 13
ttcaagtgct tcaggtcagc ctcaaag 27
<210> 14
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_MTHFR_07_REV
<400> 14
tggtctcttc atccctcgcc ttgaaca 27
<210> 15
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_01_FOR
<400> 15
gggcaattcc tcaaaaacat gtcagtgtg 29
<210> 16
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_01_REV
<400> 16
gcaatatacg ttgtcttgag aaggttgatg c 31
<210> 17
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_02_FOR
<400> 17
gcttttcaaa aagacagtca attccccaac 30
<210> 18
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_02_REV
<400> 18
gggagaatcc ctgatgtcat tcttcatag 29
<210> 19
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_03_FOR
<400> 19
gggatcaaga aactaggcaa ctggtaaaag 30
<210> 20
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_03_REV
<400> 20
ccttcttgtg ttctttgtag ctatgcagat ac 32
<210> 21
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_04_FOR
<400> 21
gttggatcat aagaaagaac acacaggaga tac 33
<210> 22
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_04_REV
<400> 22
cagcgaaagt aacttctggc ttccttcc 28
<210> 23
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_05_FOR
<400> 23
gggaagtcta agctgatttt ctagaaccca g 31
<210> 24
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_05_REV
<400> 24
gggcaaattt gacatgattt gggatagagg 30
<210> 25
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_06_FOR
<400> 25
gggttactta ccatttgcga tcacctgg 28
<210> 26
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_06_REV
<400> 26
gattgttgaa gtaccagcat gcaccatg 28
<210> 27
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_07_FOR
<400> 27
ggctgagaaa aacttttgtg gggatatgga tac 33
<210> 28
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_07_REV
<400> 28
gtgtagttgg tgtggaaatc agtgaacttg g 31
<210> 29
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_08_FOR
<400> 29
gggttcttct gagtaagaaa gattctgctc tg 32
<210> 30
<211> 36
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_08_REV
<400> 30
gagggtgctt actctaatat aaccctctat ttagtc 36
<210> 31
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_01_FOR
<400> 31
ggggacataa cagttatgat tttgcagaaa acag 34
<210> 32
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_01_REV
<400> 32
gccttctaga acagtagaca caaaacaggc 30
<210> 33
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_02_FOR
<400> 33
ggcttacctg tcttgtcttt gctgatgttt c 31
<210> 34
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_02_REV
<400> 34
gttaaggact ctgaagatgt acctatggtc c 31
<210> 35
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_03_FOR
<400> 35
gttttgtgtc tactgttcta gaaggcaaat cac 33
<210> 36
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_03_REV
<400> 36
gggacaaaag ttgtggacag gttttgaaag 30
<210> 37
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_04_FOR
<400> 37
ccagaggact ataattactc cttaatgtca gc 32
<210> 38
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_04_REV
<400> 38
gagtacagtg caatgaggga ccagtac 27
<210> 39
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_05_FOR
<400> 39
ggcaaataca caaagaaagc cctccc 26
<210> 40
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_05_REV
<400> 40
gggtgcactg taataatcca gactgtg 27
<210> 41
<211> 31
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_06_FOR
<400> 41
cctttatctg tatcaaagaa tggtcctgca c 31
<210> 42
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_06_REV
<400> 42
gactgaatat aaacttgtgg tagttggagc tgg 33
<210> 43
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_07_FOR
<400> 43
ctgaattagc tgtatcgtca aggcactc 28
<210> 44
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_KRAS_07_REV
<400> 44
gggaaggtac tggtggagta tttgatagtg 30
<210> 45
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_01_FOR
<400> 45
gtacattaga gcctctggct agggagcag 29
<210> 46
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_01_REV
<400> 46
acttcagctt ctcggtgccc actgg 25
<210> 47
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_02_FOR
<400> 47
gctcaccagg aaagcaaaga caccatgg 28
<210> 48
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_02_REV
<400> 48
gtatcaccca ggagccaggc tcactgac 28
<210> 49
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_03_FOR
<400> 49
gcaagactcc acggaagggg acagg 25
<210> 50
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_03_REV
<400> 50
gctgaaggat gaggccgtct gggag 25
<210> 51
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_04_FOR
<400> 51
catccaggaa gtgttcgggg tggaag 26
<210> 52
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_04_REV
<400> 52
ccagcatcct agagtccagt ccccac 26
<210> 53
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_05_FOR
<400> 53
ctatcaccag gtgctggtgc tgagctg 27
<210> 54
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_05_REV
<400> 54
atgccctaca ccactgccgt gattcatg 28
<210> 55
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_06_FOR
<400> 55
tcatatgggt cacacccagg gggacg 26
<210> 56
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_06_REV
<400> 56
gtgaggacgc atgtctgtcc aggcc 25
<210> 57
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_07_FOR
<400> 57
acctcatgaa tcacggcagt ggtgtagg 28
<210> 58
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_07_REV
<400> 58
gcaagaagga gtgtcagggc cggac 25
<210> 59
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_08_FOR
<400> 59
cacgggtgtc ccagcaaagt tcatgg 26
<210> 60
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_08_REV
<400> 60
ctgacaggat ggtgaccacc tcgacc 26
<210> 61
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_09_FOR
<400> 61
gatgagctca cgctgcacat ccggatg 27
<210> 62
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_09_REV
<400> 62
ctcagacatg ctcaggccaa ggggaac 27
<210> 63
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_10_FOR
<400> 63
ccatcccggc agagaacagg tcagc 25
<210> 64
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_10_REV
<400> 64
ctaccccgtt ctgtcccgag tatgctc 27
<210> 65
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_11_FOR
<400> 65
gactaccctc attcctcctg ggacgctc 28
<210> 66
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_11_REV
<400> 66
gatgagctgc taactgagca caggatgacc 30
<210> 67
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_12_FOR
<400> 67
cagccactct caccttctcc atctctgc 28
<210> 68
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_12_REV
<400> 68
gaatgctgtc cccgtcctcc tgcatatc 28
<210> 69
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_13_FOR
<400> 69
gaaagccttt tggaagcgta ggaccttgc 29
<210> 70
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_13_REV
<400> 70
gagatggctg gggcctgaga cttgtc 26
<210> 71
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_14_FOR
<400> 71
ctgcagagac tcctcggtct ctcgc 25
<210> 72
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_14_REV
<400> 72
gaagaccctt tcgccccaac ggtctc 26
<210> 73
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_15_FOR
<400> 73
gaagcgaggg tcgtcgtact cgaagc 26
<210> 74
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_15_REV
<400> 74
ggaagacaga ggacggggaa ggcgac 26
<210> 75
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_16_FOR
<400> 75
gagagatgag agtaaggggt cgccttccc 29
<210> 76
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_16_REV
<400> 76
gactgggcaa gaagtcgctg gagcag 26
<210> 77
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_17_FOR
<400> 77
ctgaccatca cccaccggag tggttg 26
<210> 78
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_17_REV
<400> 78
cacacacaca ggggtgttcc tggcg 25
<210> 79
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_18_FOR
<400> 79
cacagaccca agttgcgcaa ggtgg 25
<210> 80
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_18_REV
<400> 80
gtgggtggtg gatggtgggg ctaatg 26
<210> 81
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_19_FOR
<400> 81
ccgggtccca cggaaatctg tctctg 26
<210> 82
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_19_REV
<400> 82
gacagtggtc gtgctcaatg ggctg 25
<210> 83
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_20_FOR
<400> 83
caacagctgc ttgccttggg aacgc 25
<210> 84
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_20_REV
<400> 84
cttcggggac gtgttcagcc tgcag 25
<210> 85
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_21_FOR
<400> 85
cacattgagc acgaccaccg gcgtc 25
<210> 86
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_21_REV
<400> 86
ggttcaaata ggactaggac ctgtagtctg ggg 33
<210> 87
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_22_FOR
<400> 87
gcttctggtc cagcctgtgg tttcac 26
<210> 88
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_22_REV
<400> 88
gcaacctgct gcatgtggac ttccag 26
<210> 89
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_23_FOR
<400> 89
gacctcctcc ctcacctggt cgaagc 26
<210> 90
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_23_REV
<400> 90
gccatcttcc tgctcctggt ggacc 25
<210> 91
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_24_FOR
<400> 91
gatagagtgc agcccagcgt tggcg 25
<210> 92
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_24_REV
<400> 92
ccaggtgtgt ccagaggagc ccatttg 27
<210> 93
<211> 29
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_BRAF_01_FOR
<400> 93
ctttctagta actcagcagc atctcaggg 29
<210> 94
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_BRAF_01_REV
<400> 94
ggtgattttg gtctagctac agtgaaatct cg 32
<210> 95
<211> 28
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_BRAF_02_FOR
<400> 95
ggatccagac aactgttcaa actgatgg 28
<210> 96
<211> 33
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_BRAF_02_REV
<400> 96
gcctaaactc ttcataatgc ttgctctgat agg 33
<210> 97
<211> 104
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_01
<400> 97
gctggggact aggtacccca ttctagcggg gcacagcaca aagctcatag ggggatgggg 60
tcaccaggaa agcaaagaca ccatggtggc tgggccgggg ctgt 104
<210> 98
<211> 134
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_02
<400> 98
tggctgggcc ggggctgtcc agtgggcacc gagaagctga agtgctgcag cagggaggtg 60
aagaagagga agagctccat gcgggccagg ggctccccga ggcatgcacg gcggcctgtg 120
gggaggggag gggc 134
<210> 99
<211> 107
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_03
<400> 99
gagccgggct ccccacaggc acctgctgag aaaggcagga aggcctccgg cttcacaaag 60
tggccctggg catccaggaa gtgttcgggg tggaagcgga agggctt 107
<210> 100
<211> 92
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_04
<400> 100
cggaagggct tctcccagac ggcctcatcc ttcagcaccg atgacaggtt ggtgatgagt 60
gtcgttccct gggcagaaga tgcagggtga ga 92
<210> 101
<211> 113
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_05
<400> 101
gggtgaggag ggcgccaggc ctaccttagg gatgcggaag ccctgtactt cgatgtcacg 60
ggatgtcata tgggtcacac ccagggggac gatgtcccca aagcgctgca cct 113
<210> 102
<211> 118
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_06
<400> 102
atgtccccaa agcgctgcac ctcatgaatc acggcagtgg tgtagggcat gtgagcctgg 60
tcacccatct ctggtcgccg cacctgccct atcacgtcgt cgatctcctg ttggacac 118
<210> 103
<211> 117
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_07
<400> 103
gcatgtgagc ctggtcaccc atctctggtc gccgcacctg ccctatcacg tcgtcgatct 60
cctgttggac acggcctgga cagacatgcg tccccacaat gggtcagcac ccagggg 117
<210> 104
<211> 106
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_08
<400> 104
gcccccgcct gtacccttcc tccctcggcc cctgcactgt ttcccagatg ggctcacgct 60
gcacatccgg atgtaggatc atgagcagga ggccccaggc cagcgt 106
<210> 105
<211> 114
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_09
<400> 105
taggatcatg agcaggaggc cccaggccag cgtggtcgag gtggtcacca tcccggcaga 60
gaacaggtca gccaccacta tgcacaggtt ctcatcattg aagctgctct cagg 114
<210> 106
<211> 68
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_10
<400> 106
caccactatg cacaggttct catcattgaa gctgctctca gggttcccct tggcctgagc 60
agggccga 68
<210> 107
<211> 101
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_11
<400> 107
aacccaccac ccttgccccc caccgtggca gccactctca ccttctccat ctctgccagg 60
aaggcctcag tcaggtctcg ggggggctgg gctgggtccc a 101
<210> 108
<211> 129
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_12
<400> 108
caggaaggcc tcagtcaggt ctcggggggg ctgggctggg tcccaggtca tcctgtgctc 60
agttagcagc tcatccagct gggtcaggaa agccttttgg aagcgtagga ccttgccagc 120
cagcgctgg 129
<210> 109
<211> 98
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_13
<400> 109
cagccagcgc tgggatatgc aggaggacgg ggacagcatt cagcacctac accagacaga 60
acggggtctc aatccctcct gtgctctgcg ttcacctg 98
<210> 110
<211> 142
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_14
<400> 110
tccgcacctc gcgcagaaag cccgactcct ccttcagtcc ctcctgagct aggtccagca 60
gcctgaggaa gcgagggtcg tcgtactcga agcggcgccc gcaggtgagg gaggcgatca 120
cgttgctcac ggctttgtcc aa 142
<210> 111
<211> 101
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_15
<400> 111
ggcgcccgca ggtgagggag gcgatcacgt tgctcacggc tttgtccaag agaccgttgg 60
ggcgaaaggg gcgtcctggg ggtgggagat gcgggtaagg g 101
<210> 112
<211> 103
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_16
<400> 112
cgtcccccgc cttcccagtt cccgctttgt gcccttctgc ccatcaccca ccggagtggt 60
tggcgaaggc ggcacaaagg caggcggcct cctcggtcac cca 103
<210> 113
<211> 128
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_17
<400> 113
gcgaaggcgg cacaaaggca ggcggcctcc tcggtcaccc actgctccag cgacttcttg 60
cccaggccca agttgcgcaa ggtggagacg gagaagcgcc tctgctcgcg ccacgcgggc 120
ccatagcg 128
<210> 114
<211> 95
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_18
<400> 114
agacggagaa gcgcctctgc tcgcgccacg cgggcccata gcgcgccagg aacacccctg 60
ggggtgggac gggcacgtgc gcgtggccat gaagg 95
<210> 115
<211> 117
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_19
<400> 115
tccccaccgc tgcttgcctt gggaacgcgg cccgaaaccc aggatctggg tgatgggcac 60
aggcgggcgg tcggcggtgt cctcgccgtg ggtcaccagc gcctcgcgca cggccgc 117
<210> 116
<211> 125
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_20
<400> 116
ggcccgaaac ccaggatctg ggtgatgggc acaggcgggc ggtcggcggt gtcctcgccg 60
tgggtcacca gcgcctcgcg cacggccgcc agcccattga gcacgaccac cggcgtccag 120
gccag 125
<210> 117
<211> 85
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_21
<400> 117
caggccagct gcaggctgaa cacgtccccg aagcggcgcc gcaactgcag agggagggtc 60
agggcctctt gtcaagccag gatcc 85
<210> 118
<211> 95
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_22
<400> 118
ccaccaccca tgtttgctgg tggtggggca tcctcaggac ctctgccgcc ctccaggacc 60
tcctccctca cctggtcgaa gcagtatggt gtgtt 95
<210> 119
<211> 105
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_23
<400> 119
agtatggtgt gttctggaag tccacatgca gcaggttgcc cagcccgggc agtggcaggg 60
ggcctggtgg gtagcgtgca gcccagcgtt ggcgccggtg catca 105
<210> 120
<211> 89
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_CYP2D6_24
<400> 120
ccggtgcatc aggtccacca ggagcaggaa gatggccact atcacggcca ggggcaccag 60
tgcttctagc cccatacctg cctcactac 89
<210> 121
<211> 96
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_01
<400> 121
attttatttt atctatgtct catttacttt tctgtaagta gatataactt ttcaaaaaga 60
cagtcaattc cccaactttt atgtcgttct tcaaaa 96
<210> 122
<211> 91
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_02
<400> 122
ttttatgtcg ttcttcaaaa gcatcaacct tctcaagaca acgtatattg catattttac 60
ctgaaacaag aaagagtaac atgttaaaat a 91
<210> 123
<211> 100
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_03
<400> 123
aaaaaaaaaa aacccaacaa ctttacctgg atgtttagtt ggatcataag aaagaacaca 60
caggagatac tgaaacttct ttcccaggag ggaaaacatt 100
<210> 124
<211> 112
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_04
<400> 124
tgaaacttct ttcccaggag ggaaaacatt gtatctgcat agctacaaag aacacaagaa 60
ggtatttgtt acatttctct acacaggcaa aggctggagg gacaaaaggc ag 112
<210> 125
<211> 95
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_05
<400> 125
aaaaagtata gtatactaaa aaattaagac agctaaacaa aaaaagaaaa attacttacc 60
atttgcgatc acctggattg atggcaacta atgct 95
<210> 126
<211> 126
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_06
<400> 126
attgatggca actaatgctc ctctatccca aatcatgtca aatttgccaa tatttgtcct 60
accagaaaga gaaaaaacat tttatgggag aaaaatcaaa tctttaagaa gatgagcagc 120
gtcccc 126
<210> 127
<211> 108
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_07
<400> 127
aattatttac ccaaatcaaa acaaacctta aatactttgg ttccaggaat ttcggtgatt 60
ggttcttctg agtaagaaag attctgctct gtaaaaaatt cttgtatc 108
<210> 128
<211> 111
<212> DNA
<213> Artificial Sequence
<220>
<223> >520999_TPMT_08
<400> 128
taaaaaattc ttgtatccca agttcactga tttccacacc aactacactg tgtccccggt 60
ctgcaaacct gcataaaatc atacatttac acttaaatta tgttttcaaa t 111
Claims (11)
- 암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 프라이머를 이용하여 시료의 DNA로부터 표적 부위를 증폭하는 단계;
상기 증폭된 산물에 어댑터 (adapter)를 부착하는 단계;
상기 어댑터가 부탁된 산물을 증폭하여 최종 산물을 제조하는 단계; 및
상기 최종 산물의 서열을 분석하여 상기 표적 부위에 대한 앰플리콘 (amplicon)의 서열과 비교하여 유전자 변이를 검출하는 단계
를 포함하는 것을 특징으로 하는, 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(NGS) 방법. - 제1항에 있어서,
상기 암 유전자 또는 항암제 대사관련 유전자는 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것을 특징으로 하는, 방법. - 제1항에 있어서,
상기 표적 부위는 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것을 특징으로 하는, 방법. - 제1항에 있어서,
상기 프라이머는 표 2에 기재된 서열번호 1 내지 96의 프라이머로부터 선택되는 것을 특징으로 하는, 방법. - 제1항에 있어서,
상기 앰플리콘은 표 3에 기재된 서열번호 97 내지 128의 서열로부터 선택되는 것을 특징으로 하는, 방법. - 제1항에 있어서,
상기 증폭된 산물을 정제하는 단계를 추가로 포함하는 것을 특징으로 하는, 방법. - 암 유전자 또는 항암제 대사관련 유전자의 표적 부위를 증폭할 수 있는 프라이머를 포함하는 것을 특징으로 하는, 시료의 DNA로부터 표적 부위를 증폭하기 위한 조성물.
- 제7항에 있어서,
상기 암 유전자 또는 항암제 대사관련 유전자는 표 1에 기재된 유전자로부터 선택되는 1종 이상인 것을 특징으로 하는, 방법. - 제7항에 있어서,
상기 표적 부위는 표 1에 기재된 표적 부위로부터 선택되는 1종 이상인 것을 특징으로 하는, 방법. - 제7항에 있어서,
상기 프라이머는 표 2에 기재된 서열번호 1 내지 96의 프라이머로부터 선택되는 것을 특징으로 하는, 방법. - 제7항에 따른 조성물, 어댑터 및 바코드 프라이머를 포함하는 것을 특징으로 하는, 암 유전자 및 항암제 대사 관련 유전자 변이 진단 패널.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170179123A KR20190078715A (ko) | 2017-12-26 | 2017-12-26 | 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 |
| KR1020230128858A KR20230146139A (ko) | 2017-12-26 | 2023-09-26 | 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 |
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020170179123A KR20190078715A (ko) | 2017-12-26 | 2017-12-26 | 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 |
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| KR1020230128858A Division KR20230146139A (ko) | 2017-12-26 | 2023-09-26 | 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 |
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| KR1020230128858A KR20230146139A (ko) | 2017-12-26 | 2023-09-26 | 암 유전자 및 항암제 대사 관련 유전자 변이 분석을 위한 차세대 염기 서열 분석(ngs) 기반 혼합 진단 패널 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154872A (zh) * | 2019-12-31 | 2020-05-15 | 浙江大学 | 用于检测肺癌驱动性基因突变的探针及试剂盒 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111154872A (zh) * | 2019-12-31 | 2020-05-15 | 浙江大学 | 用于检测肺癌驱动性基因突变的探针及试剂盒 |
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