KR20190054999A - Portulaca oleracea L. porridge composition for promting osteogenesis - Google Patents

Abstract

The present invention relates to a Portulaca oleracea L. porridge composition having an osteogenesis promoting activity. The composition has low toxicity in the body by comprising natural substances such as Portulaca oleracea L. and rice, and has excellent osteogenesis promoting ability, thereby being able to be useful for growing and developing of young children, and preventing and treating of bone diseases.

Description

[0002] Portulaca oleracea L. porridge composition for promising osteogenesis < RTI ID = 0.0 >

The present invention relates to a novel hyaluronic acid composition having a bone growth promoting activity, which utilizes the information of the original horses described in the old documents to discover new functional foods.

It is commonly referred to as a food that is made by pouring enough water into a dead grain and spreading it so that it becomes soft or semi-liquid. In particular, it has been recognized as a treatment type and a patient type because of its familiar and digestive nature as a dead traditional Korean food. Nowadays, as the kind of porridge gradually increases according to the desire of consumers, the size of the porridge market becomes larger, have.

In addition, it can supplement various nutrients at the same time according to the addition of the dead material. It has nutritional supply, relieves thirst, improves the constitution, improves physical strength, and also has diuretic effect and weight reduction It is known to be effective in digestion and absorption of other foods.

On the other hand, there are many cases in which the knowledge of the ancestors handed down from ancient times is proved to be excellent in the whole life, and it is predicted that it will be possible to apply the knowledge of the ancestors to the improvement of modern diseases or lifestyle- Therefore, it is necessary to develop food using herbal ingredients derived from the ancient books and dietary information.

In other words, based on the historical records of the royal records such as the intention book and the companion diary, we can extract the modern processing and information about the royal witty information and the prescription, and manage and help the health threat factors that may arise in the new lifestyle.

In the literature of the new medicine and the first star, various kinds of porridge using herbal materials are traditionally recorded.

Specifically, the secret of washing is introduced with porridge made from Purses (Machi), and it is said that it has the effect of treating the pussy-dying disease (paralytic disease, joints and flesh and skin disease) and eliminating the boil.

As described above, only the effect of eliminating dead non-diseases and boils using a marjoram, which is traditionally used, has been recorded. The inventors of the present invention have found that the marjoram has an effect of promoting bone growth unlike the conventional records.

Korean Patent No. 1,577,792 Korean Patent Publication No. 2017-0025359

It is an object of the present invention to provide a marigold ceramics composition having a bone growth promoting activity including marigold and rice.

It is another object of the present invention to provide a pre-mix comprising the marjoram body composition.

Still another object of the present invention is to provide a retort food comprising the above-mentioned marchia porridge composition.

In order to accomplish the above object, the present invention provides a marinated porridge composition comprising marigold and rice.

The marjoram body composition may further include at least one member selected from the group consisting of a top, a defibrillator, a black beard, red bean, and a pickle.

The marjoram body composition may further include at least one member selected from the group consisting of jujube and jujube.

The marigold may be a defatted marigold.

The degreased marigold may be one in which fat has been removed by solvent extraction using hexane or acetone.

The marjoram body composition may be mixed at 20 to 80 parts by weight with respect to 100 parts by weight of rice.

The rice may be rice, glutinous rice, sweet potato or mixtures thereof.

The composition comprises 10 to 40 parts by weight of the top layer, 10 to 50 parts by weight of the defoliant, 10 to 50 parts by weight of black beef, 5 to 40 parts by weight of red beans, and 1 to 40 parts by weight of the bean jam based on 100 parts by weight of rice. Or more species.

The marjoram body composition may further comprise at least one member selected from the group consisting of 1 to 30 parts by weight of jujube and 1 to 30 parts by weight per 100 parts by weight of rice.

The marjoram body composition may further include at least one selected from the group consisting of carrot, onion, garlic and red pepper.

The marjoram body composition may further comprise at least one selected from the group consisting of salt, soy sauce, sesame oil, and sesame leaf.

In order to accomplish the above-mentioned other objects, the present invention also provides a porcelain premix comprising the marinated porcelain composition.

According to another aspect of the present invention, there is provided a retort food composition comprising the marjoram body composition.

The present invention relates to a method for preventing and treating osteoporosis, comprising the steps of: (a) providing a bone marrow-derived bone marrow-derived bone marrow-derived bone marrow-derived bone marrow- .

In addition, the composition of the present invention is effective alone or in combination with growth hormone therapy and concurrent therapies to treat key growth.

The present invention relates to a marigold porridge composition having bone growth promoting activity including marigold and rice.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing a marine porridge having bone growth promoting activity. The method for preparing a marinated porridge according to the present invention may further comprise at least one selected from the group consisting of upper limbs, donors, black beaks,

In addition, the marine porridge composition of the present invention may further comprise jujube, chumaza, or a mixture thereof.

It is reported that the Maichijun (馬 齒 ??) is an outpost of Portulaca oleracea L. and is effective for antimicrobial activity, intestinal interactions due to intestinal smooth muscle contraction, diuretic effect.

The marigolds should be defatted, not dried or extracted, to further enhance bone growth promoting activity. The fat is extracted and removed while minimizing denaturation of the protein contained in the marigold, and the residual oil content is lowered to a desired level to prepare a degreasing agent suitable for use as a protein extraction raw material. In the present invention, the method for degreasing marigolds is not particularly limited as long as the residual oil content can be minimized, but a solvent extraction method using hexane or acetone (solvent) is preferably used. In the solvent extraction method, the solvent is extracted with circulating oil by adding 2 to 4 times of the solvent to the weight of the dried crude ash until the residual oil content becomes 5 wt% or less, and the residual solvent is removed at 60 ° C or lower Process can be applied.

The content of the marigold is 20 to 80 parts by weight based on 100 parts by weight of rice. When the content of marigold is less than the lower limit value, the bone growth promoting activity may be insignificant, and in the case of exceeding the upper limit value, the functionality may be lowered.

In addition, the upper limb is a medicinal herb of Morus alba L. or a young branch of the genus Morus alba, and has been reported to have a medicinal action, an antimicrobial action, an intestinal motility inhibitory action, and a hypotensive effect.

The upper limb can be used in conjunction with marjoram to enhance bone growth promoting activity. The upper limb is used in an amount of 10 to 40 parts by weight. When the content of the upper limb is less than the lower limit, the bone growth promoting activity may not be improved. When the upper limit is exceeded, the sensory properties are lowered, .

In addition, the term "Cassia obtusifolia L." means the seed of the deficiency, and it has functions such as blood pressure lowering, diuretic, troubled, uterine contraction, skin fungus suppression, cholesterol lowering.

The cleanser enhances the sensory properties and can be used together with horseshoe to enhance bone growth promoting activity. When the content of the cleanser is less than the lower limit, the sensory properties and the bone growth promoting activity may not be improved. If the content exceeds the upper limit, the sensory properties are deteriorated and the consumer is not preferred Color.

In addition, the black beans are black beans with a dark brown color, and have many anti-aging ingredients, and are effective in prevention of adult diseases and dieting.

The black pot can enhance the sensory properties and can be used together with the marigold to improve the bone growth promoting activity. The black black seeds are used in an amount of 10 to 50 parts by weight, and when the content of black seeds is less than the lower limit, the activity and the bone growth promoting activity may not be improved.

The red bean is an annual herbaceous plant with roots of dicotyledonous plant rosemary, and is effective for each disease.

The red bean enhances the sensory properties and can be used together with marjoram to improve bone growth promoting activity. The bean paste is used in an amount of 5 to 40 parts by weight. When the content of red bean is less than the lower limit, the activity and the bone growth promoting activity may not be improved, and the bean paste may be killed if it exceeds the upper limit.

The chewing gum is a medicinal substance which is saponin, dementia, cyanosis, diuretic, bloody blood, detoxification.

The rider may be used in combination with horseradish to improve bone growth promoting activity. Such a chewing gum is used in an amount of 1 to 40 parts by weight, and when the content of the chewing gum is exceeded, the bone growth promoting activity may not be improved.

In addition, the jujube is a fruit of jujube, and is used as diuretic, tonic, and emollient. The jujube is more mature than the one used alone; And a mixture of at least one selected from the group consisting of the upper limb, the donor, the black beak, the red bean, and the pickle, and exhibits further improved bone growth promoting activity.

The jujube is used in an amount of 1 to 30 parts by weight. When the content of jujube is less than the lower limit, the activity and the bone growth promoting activity may be insignificant. If the jujube exceeds the upper limit, the functionality may be lowered.

In addition, Chumma means "Samseong", which is said to be planted in late spring, and it is effective for symptoms of hyperhidrosis, fever, and asthenopia. The above-mentioned syllable is more likely to be used than Machino alone; And a mixture of at least one selected from the group consisting of the upper limb, the donor, the black beak, the red bean, and the pickle, and exhibits further improved bone growth promoting activity.

Such chimpanzee is used in an amount of 1 to 30 parts by weight, and when the content of chum extract is out of the above range, the bone growth promoting activity may be insignificant.

In order to enhance the functionality of the present invention, one or more selected from the group consisting of carrot, onion, garlic and red pepper; And / or one or more selected from the group consisting of salt, soy sauce, sesame oil and sesame leaf.

In addition, the present invention can provide a premix for a moxibustion comprising a marigold-shaped porridge composition having bone growth promoting activity.

The pomace premix is a freeze-dried composition of the above-mentioned mashikhin porridge, and can be easily cooked by adding water to enjoy the texture and taste similar to the cooked porridge.

As a method for producing the above-mentioned pre-mix, a conventional method can be used.

In addition, the present invention can provide a retort food comprising a marigold porridge composition having bone growth promoting activity.

The above-mentioned retort food is obtained by putting the above-mentioned marjoram body composition into a container and then sterilizing the retort, and it is possible to enjoy the texture and taste similar to the cooked porridge just by heating.

As the method for producing the above-mentioned dough retort food, a usual method can be used.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Production Example 1. Degreasing

Three times as much n-hexane as the weight of the dried marjoram was added thereto, followed by circulating oil extraction. Extraction was carried out until the total oil content reached 4% by weight, and the residual hexane was removed at 55 캜 to obtain a degreasing step.

Control group. Rice mingling

100 parts by weight of rice was roasted and then boiled rice was roasted. Then, water was poured and boiled in high heat. Then, when boiled, the rice was minced by gradually boiling and gradually boiling.

Example 1

100 parts by weight of rice was added, and then boiled rice was mixed with 60 parts by weight of defatted rice bran in Preparation Example 1 and roasted together. Then, water was poured and boiled in high heat. When the boiled rice was boiled, the rice was gradually boiled while gradually boiling.

Example 2 < RTI ID = 0.0 >

Except that 100 parts by weight of rice was added to 60 parts by weight of defatted rice bran in Preparation Example 1 and 20 parts by weight of the top of the rice bran in the same manner as in Example 1 except that 100 parts by weight of rice was added.

Example 3: Machino-Hygiene composition + upper limb + jujube

100 parts by weight of rice was added to 100 parts by weight of rice, and then 60 parts by weight of germinated brown rice prepared in Preparation Example 1, 20 parts by weight of upper body and 15 parts by weight of jujube were added to rice bran.

<Test Example>

The mashii porridge composition prepared in the above example was crushed and lyophilized to be used as a sample.

Cell experiment

The osteosarcoma cell line MG63 cells were cultured in a DMEM culture medium containing 10% FBS, 100 unit / ml penicillin, 100 mg / ml streptomycin, and incubated at 37 ° C in a 5% CO ₂ incubator.

Test Example 1. Cytotoxicity measurement

Osteoblast cells were plated in 96-well plates at a concentration of 1 × 10 ⁴ cells / well, and 200 μl of DMEM medium containing 10% FBS was added thereto and cultured in an incubator. When the cells were over 70% in each well, the culture medium was removed and cultured under the same conditions as the control and treatment groups. After 24 hours, 10 μl of 4- [3- (4-iodophenyl) -2- (4-nitrophenyl) -2H-5-tetrazolio] -1,3-benzene disulfonate (WST) for a time and cultured in a CO ₂ incubator. The absorbance was then measured at 450 nm using an ELISA reader.

After 24 hours, the composition of the porridge was treated with MG63 cells, and the absorbance was compared with that of the control. As a result, all the porcelain porcelain compositions prepared according to Examples 1 to 3 exhibited a range of 95 to 120%, indicating no cytotoxicity .

Animal experiment

Experimental animal

When applied to 148 children with early maturity, the female hormone was decreased while the growth hormone was increased. Based on the clinical results of the average height of 0.6 ㎝, To evaluate the effect of the test.

18 g 4-week-old white rats (Joongang Animal Lab, Seoul, Korea) were used for the experiment. The mice were fed a free diet in a breeding room controlled at 22 ± 1 ° C and 55 ± 10% humidity throughout the experiment and the light and dark cycles were adjusted at 12 hour intervals. All animal experiments were handled in accordance with Korea Food Research Institute animal experiment management regulations.

For the administration of the samples, mice were randomly divided into 10 rats and fed with the diet for 4 weeks. The CD (Chow diet) was a negative control with a diet of normal (20% fat), and the HFD and sample groups were autologically fed on a high fat diet (43% fat containing, Teklad Laboratories, Madison, WI, USA) . Samples were orally administered to the control and the compositions of Examples 1 to 3 daily at a concentration of 1.4 parts by weight and 4.2 parts by weight for 100 parts by weight of the high-fat diet, and positive control was 20 ug / kg BW / day (subcutaneous injection). The CD and HFD groups were orally administered the same amount of distilled water for the same period.

-5 mice -

Group 1 (CD, negative control): ingestion of normal diet, drinking water instead of composition

Group 2 (HFD, negative control): ingestion of high fat diet, drinking water instead of composition

Group 3 (growth hormone, positive control): high fat diet, growth hormone 20 ug / kg BW / day

Group 4 (Example 1_ low): ingested in a high fat diet, 1.4 parts by weight of the composition of Example 1

Group 5 (Example 1_High): High fat diet intake, 4.2 parts by weight composition of Example 1

Test Example 2 Measurement of Length Change of Femur and Tibia

The length of the femur (from the knee to the hip joint) and the length of the tibia (from the knee to the foot joint, tibia) were measured by DEXA (Dual Energy X-ray Absorptiometry) The lengths measured for 4 weeks by measuring the length are shown in Table 1 below.

division Femur (mm) Tibia (mm) Group 1 (CD) 4.9 ^a 9.2 ^b Group 2 (HFD) 4.0 ^b 8.1 ^c Group 3 (growth hormone) 5.1 ^a 10.5 ^a Group 4 (Example 1_ low) 4.9 ^a 9.8 ^a Group 5 (Example 1_g) 5.0 ^a 10.1 ^a

As shown in Table 1 above, it was confirmed that the overall length of the tibia was longer than that of the femur in all groups.

The growth of the femur and tibia was similar to that of the group 3 (growth hormone group), and the group 1 (normal diet group), group 2 (high fat diet group) The length was increased.

Test Example 3. Measurement of Serum ALP Concentration

Serum ALP indicates the degree of destruction of osteocytes. The degree of induction of ALP was measured using a blood biochemical instrument (7170S, Hitachi, Tokyo, Japan) using the serum obtained by sacrificing mice used in the experiment.

division ALP (IU / L) Group 1 (CD) 27.1 ^b Group 2 (HFD) 28.4 ^a Group 3 (growth hormone) 21.6 ^c Group 4 (Example 1_ low) 26.1 ^b Group 5 (Example 1_g) 22.7 ^c

As shown in Table 2 above, the groups in which the marigold composition prepared according to Example 1 of the present invention showed a decrease in serum ALP concentration-dependently compared to the group 1 (normal diet group) and 2 (high fat diet group) , Which is similar to the number of injected growth hormone.

Claims (13)

Wherein the composition comprises bone marrow-derived growth promoting activity. [3] The composition according to claim 1, wherein the composition comprises at least one member selected from the group consisting of a top, a defibrillator, a black beak, a red bean, and a pickle. [3] The composition of claim 1 or 2, wherein the composition comprises at least one selected from the group consisting of jujube and jujube. [3] The composition according to claim 1, wherein the marigold is defatted marigold. [Claim 5] The method according to claim 4, wherein the degreased marigolds are fat-removed by a solvent extraction method using hexane or acetone. [3] The composition according to claim 1, wherein the gherkin-like porridge composition is mixed at 20 to 80 parts by weight with respect to 100 parts by weight of rice. [3] The composition of claim 1, wherein the rice is rice, glutinous rice, sweet potato or a mixture thereof. [Claim 3] The method according to claim 2, wherein the marjoram body composition comprises 10 to 40 parts by weight of the upper part, 10 to 50 parts by weight of the defoliant, 10 to 50 parts by weight of black beef, 5 to 40 parts by weight of red bean, Wherein the composition further comprises at least one member selected from the group consisting of liposomes, liposomes, and liposomes. [3] The method of claim 1, wherein the marjoram body composition further comprises at least one member selected from the group consisting of 1 to 30 parts by weight of jujube and 1 to 30 parts by weight per 100 parts by weight of rice. &Lt; / RTI &gt; [3] The composition according to claim 1, wherein the composition comprises at least one selected from the group consisting of carrots, onions, garlic and red pepper. [3] The composition according to claim 1, wherein the marjoram body composition further comprises one or more selected from the group consisting of salt, soy sauce, sesame oil, and sesame leaf. A premix for a pot containing a marigold porridge composition according to claim 1. A retort food comprising the hatching porridge composition according to claim 1.