KR20190047211A - A composition for hepatoprotective or relieving hangover comprising herbal extracts complex and l- aspartic acid - Google Patents

Abstract

The present invention relates to a composition for protecting liver or relieving hangover comprising a complex herbal extract and L-aspartic acid as active ingredients. The composition of the present invention comprises: a complex herbal extract consisting of a Pueraria lobata extract and an Artemisia capillaris extract; and an L-aspartic acid as active ingredients. According to the present invention, an effect of preventing and treating alcoholic liver diseases such as alcoholic hepatitis, alcoholic fatty liver, hepatocirrhosis, and liver cancer is excellent.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for liver protection or hangover comprising a herbal extract and L-aspartic acid as an active ingredient. BACKGROUND ART < RTI ID = 0.0 >

The present invention relates to a composition for liver protection or hangover decomposing which comprises a complex herbal extract and L-aspartic acid as an active ingredient.

From ancient times, liquor has been accompanied by human history, and it has been used in our daily lives as a medicinal product or as a favorite food to be loved. Alcohol, which is the main constituent of alcohol, shows various pharmacological effects when consumed. It has positive effects such as appetite enhancement, smooth circulation, elevated body temperature, stress relieving, and induction of sleep, while it has an addiction to alcohol, And the like.

Alcohol is a cause of myocardial infarction and hypertension as well as gastrointestinal problems such as gastritis, gastric ulcer, alcoholic hepatitis, and alcoholic fatty liver, and acts as a cause of problems such as alcoholic dementia and alcoholic poisoning. It can cause health problems.

In addition, excessive stress and wrong drinking habits cause social, economic, and human damage as well as health problems, crime and juvenile problems, traffic accidents, etc. Therefore, proper use of alcohol is required.

Alcohol is absorbed and metabolized by the liver, which is metabolized mainly by the pathway of alcohol dehydrogenase (ADH). Alcohol is converted to acetaldehyde by ADH, which is a toxic substance that causes cell wall damage and cell necrosis, and also causes a hangover that occurs when a large amount of alcohol is ingested.

Acetaldehyde is usually degraded to acetaldehyde dehydrogenase (ALDH) and eventually decomposed into CO ₂ and H ₂ O, or into the citric acid cycle for energy production. However, when alcohol is consumed excessively, NADH produced from nicotinamide adenine dinucleotide (NAD), which acts as a coenzyme and electron transfer receptor when alcohol is metabolized to acetaldehyde and acetyl-CoA, enters the mitochondria and oxidizes, It destroys the balance.

This leads to accumulation of cholesterol in the liver, protein synthesis, and fatty liver and hyperlipemia. In addition, acetaldehyde induces accumulation of fatigue substances such as lactic acid, inhibits vitamin activation, reduces the amount of vitamin in the blood, and releases urine and sweat a large amount of water and various electrolytes into the body to cause thirst, headache and lethargy do.

Therefore, a lot of trouble occurs in daily life the next day after being overcooked, and various foods have been used for detoxification of such alcohol. For example, he has eaten pine nuts, bean sprouts soup, bamboo soup noodle soup, cattle soup, and burger soup. In one room, he has also eaten garbage, citron tea and green tea.

The present inventors have intensively developed a composition derived from natural products that is safe for human body by using a herbal medicine having an effect of improving liver disease and hangover caused by alcohol.

The present invention relates to a herbal composition comprising a crude herb extract comprising a garlic extract, a prince extract, a sine extract and a dermis extract; And L-aspartic acid as an active ingredient.

According to one aspect of the present invention, there is provided a herbal composition comprising a herbal extract comprising a Galangal extract and a Prince extract; And L-aspartic acid as an active ingredient.

In one embodiment, the complex herbal medicine extract may comprise 5 to 9 parts by weight of garlic extract and 2 to 3 parts by weight of a prick extract.

In one embodiment, the herbal extract may further comprise a sine extract or a dermis extract.

In one embodiment, the complex herbal medicine extract may further comprise 0.1 to 0.3 parts by weight of a sine extract.

In one embodiment, the herbal extract may further comprise 0.5 to 0.9 parts by weight of a dermis extract.

In one embodiment, the herbal extract may be extracted from one or more selected solvents selected from the group consisting of distilled water, 50% (v / v) ethanol and 95% (v / v) ethanol.

In one embodiment, the composition may comprise from 2 to 4 parts by weight of the L-aspartic acid.

According to another aspect of the present invention, there is provided a pharmaceutical composition for the prevention and treatment of alcoholic liver disease comprising the composition.

In one embodiment, the alcoholic liver disease can be one or more of the group consisting of alcoholic hepatitis, alcoholic fatty liver, liver cirrhosis and liver cancer.

According to another aspect of the present invention, there is provided a pharmaceutical composition for hangover use comprising the composition.

According to another aspect of the present invention, there is provided a health functional food for preventing and improving an alcoholic liver disease comprising the composition.

According to another aspect of the present invention, there is provided a health functional food for hangover habit comprising the composition.

According to the present invention, the composition is excellent in the prevention and treatment of alcoholic liver diseases such as alcoholic hepatitis, alcoholic fatty liver, liver cirrhosis and liver cancer.

Particularly, the composition has an effect of reducing the alcohol concentration in the blood, and is excellent in preventing and treating the liver disease caused by alcohol, and has excellent hangover resolution effect.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIG. 1 is a graph showing lipid peroxidation of liver tissues in a mouse model to evaluate the effect of the composition of the present invention on the prevention of alcoholic liver disease. FIG.
HJS001, HJS002, and HJS003 were the experimental group that ingested the composition according to one embodiment of the present invention, and Sylimarin was the control group consuming the alcohol and silymarin It is a control group.
FIG. 2 is a graph showing a clinical evaluation of the effect of inhibiting blood alcohol concentration on the composition of the present invention. FIG.
Control was a change in blood alcohol concentration at 15, 30, 60, 90, 120, 180, and 240 minutes after drinking of the subjects. HJS003 was administered at 15, 30, 60, 90, 120, 180, and 240 minutes.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

When an element is referred to as " comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

Various scientific dictionaries, including the terms contained herein, are well known and available in the art. Although any methods and materials similar or equivalent to those described herein are found to be used in the practice or testing of the present application, some methods and materials have been described. It is not intended that the invention be limited to the particular methodology, protocols, and reagents, as they may be used in various ways in accordance with the context in which those skilled in the art use them.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

According to one aspect of the present invention, there is provided a herbal composition comprising a herbal extract comprising a Galangal extract and a Prince extract; And L-aspartic acid as an active ingredient. The complex herbal medicine extract may further comprise a sine extract or a dermis extract.

The above-mentioned " comprising as an active ingredient " may indicate an effect of improving liver disease and hangover, and may include an effective amount of decreasing the blood alcohol concentration elevated by alcohol consumption.

The " Pueraria lobata ) "is a flower of the 칡, and it is dried in the sun by cutting off the flower of 칡 에 in late summer or early autumn. It is an uneven or long oval or bell-shaped flower with a length of 0.5 to 1.5 cm, a width of 0.2 to 0.6 cm and a thickness of 0.2 to 0.3 cm. The calyx is dark green with the lower part connected, the end divided into 5 parts, the two appear to be combined and pointed. The outer surface is covered with yellowish white hairs. When viewed with a magnifying glass, the number of petals is 5, and it is covered with calyx. There are 10 stamens and pistils are thin, long, curved, and have fine hairs. There is a peculiar smell and taste is slightly sweet. The headache, fever, fever and chest tightness are uncomfortable, so it can not keep limbs, thirst, anorexia, abdominal distension and nausea.

The " Artemisia & capillaris "is a medicinal plant which dried the top part of the matsutake mushroom. It is a perennial plant of the Asteraceae family. It has a lower part of the stems and becomes a semi-class. The stem of the flower is short and the upper part is a rosette.

The above-mentioned asthma is caused by jaundice caused by moist heat (acute hepatitis), and it is used for symptoms such as fuming, whole body yellow, red urine, and less symptoms. It is also used for chronic hepatitis, liver cirrhosis, liver cancer, cholecystitis, and gallbladder stones. And it is also used for the symptom of burning of the skin due to skin diseases such as eczema, omnis, ringworm, rubella, and burning fever. It has been reported that the pharmacological action is to promote bile secretion, hepatic function regeneration, hepatocyte regeneration, lipolysis, coronary artery dilatation, hypotensive action, antipyretic, diuretic, antimicrobial and experimental carcinoma cells.

The "sign (Amomum villosum ") is a medicinal plant made from fruits of Yangchun, which is a perennial herbaceous plant belonging to the ginger family. Cine is oval, 1 ~ 2cm in length, and 1 ~ 1.5cm in diameter. Outer surface is dark brown and unclear, but there are three dull ridgelines and 3 ~ 7 are attached to the axis. It also has short, spiny protuberances, it is flexible, weak and easy to cut, and has a sense of pricking when touched by hand. There is a sharp residue of fur on the top, and a base on the base. The fruit skin is thin and soft, and on the inner surface, a clear vascularity can be seen. The taste of the sign is slightly strong and warm. It is effective for the person who complains about the digestive system and does not have good appetite. It is used for digestion and vomiting due to gastrointestinal dysfunction. It is also used when the stomach is cold and it causes pain by eating cold drinks and food. Especially, in summer, the lower abdomen shows excellent efficacy for the symptoms that cause cold and diarrhea in succession.

The term " Citrus unshiu " refers to the mature rind of the mandarin or mandibular relative plants of the oriental . Its appearance is unevenly shaped shell, outer side is yellowish red, dark tan, and there are many small concave marks due to loss. The inside is white or pale grayish brown, and the vagina is light and easy to crumble. The dermis releases loose glands and strengthens the function of the spleen, treating only the abdominal cavity, trimming, vomiting, nausea, dyspepsia, sluggishness, lingering symptoms, and thin feces. Seawater, sputum and diuretic action. It is a medicine that the effect of medicine increases with the reading aloud, mahwang, dandelion, sour oil, Pharmacological action has been reported to cause the digestive tract stimulation, digestion promotion, geomorphic, anti-ulcer, antidiarrheal secretion, hypertension, elevated blood pressure, antiallergic, bile secretion promotion, uterine smooth muscle suppression,

The complex herbal medicine extract may comprise 5 to 9 parts by weight of the garlic extract and 2 to 3 parts by weight of the granular extract, and the complex herbal medicine extract may further comprise 0.1 to 0.3 parts by weight of the sine extract and 0.5 to 1.0 part by weight of the dermis extract . In addition, the composition may comprise 2 to 4 parts by weight of the L-aspartic acid.

When the variation of the mixing ratio is large, the composition may not properly realize the effect derived from the active ingredient of each component, so that the mixing ratio can be appropriately controlled in consideration of the working environment and the quality of the final product. The inventors intend to derive the optimum mixing ratio of the raw materials on the basis of a number of experimental results, and the effect of the composition can be maximized.

According to another aspect of the present invention, there is provided a pharmaceutical composition for the prevention and treatment of alcoholic liver diseases or a pharmaceutical composition for hangover disease comprising the composition.

The alcoholic liver disease may be one or more of the group consisting of alcoholic hepatitis, alcoholic fatty liver, liver cirrhosis and liver cancer.

The " prevention " means any action that reduces the frequency or extent of the pathological event. Prevention can be complete or partial. In this case, the symptom of liver disease in the subject may be reduced as compared with the case where the composition is not used.

The term " treatment " means any clinical intervention to alter the natural course of a subject or cell to be treated, and may be performed during or during the course of a clinical pathology. The desired therapeutic effect may be to prevent the occurrence or recurrence of the disease, to alleviate the symptoms, to reduce all direct or indirect pathological consequences of the disease, to prevent metastasis, to reduce the rate of disease progression, Relieving or temporarily alleviating the condition, or improving the prognosis.

The compositions can be administered in the form of oral delivery, parenteral delivery. The composition may be administered systemically or topically, and the administration may include oral administration and parenteral administration. The composition may be formulated with a suitable amount of a pharmaceutically acceptable vehicle or carrier to provide a suitable dosage form.

The composition may further comprise a carrier, an excipient and a diluent used in the preparation of the pharmaceutical composition. Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, But are not limited to, cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate or mineral oil.

In addition, the composition may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like, external preparation, suppository and sterilized injection solution.

The solid preparation for oral administration may be a tablet, a pill, a powder, a granule, a capsule, etc. The solid preparation may be prepared by adding to the compound and its fractions at least one or more excipients such as starch, calcium carbonate, sucrose, lactose , Or gelatin. In addition to the above excipients, lubricants such as magnesium stearate and talc may be used.

The liquid preparation for oral administration may be a suspension, a solution, an emulsion, a syrup, etc. In addition to water and liquid paraffin which are simple diluents, various excipients such as wetting agents, sweeteners, fragrances and preservatives may be used.

The preparation for parenteral administration may be a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a lyophilized preparation, or a suppository. Examples of the non-aqueous solutions and suspensions may include injectable esters such as propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, and glycerogelatin.

The composition may be administered in a pharmaceutically effective amount sufficient to treat the disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the species and severity, age, sex, disease type, Activity, sensitivity to the drug, time of administration, route of administration and rate of release, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts.

The composition may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art.

The preferred dosage of the composition may vary depending on the condition and the weight of the patient, the severity of the disease, the type of drug, the route of administration and the period of time, and the appropriate total daily dose may be determined by the treatment within the proper medical judgment range . Generally, an amount of 0.001 to 1000 mg / kg, preferably 0.05 to 200 mg / kg, more preferably 0.1 to 100 mg / kg, can be administered in a single dose, divided into several times a day.

And is not particularly limited as long as it is an object for the prevention or treatment of the liver disease, and any object can be applied. For example, the composition can be applied to any individual such as a monkey, dog, cat, rabbit, guinea pig, rat, mouse, cattle, sheep, pig, Methods include, without limitation.

According to another aspect of the present invention, there is provided a health functional food for preventing or ameliorating an alcoholic liver disease comprising the composition, or a health functional food for hangover marrow.

The health functional food refers to a food prepared and processed by using raw materials or ingredients having useful functions in the human body according to the Act on Health Functional Foods. The functional properties include nutrients for the structure and function of the human body, ≪ / RTI > may be meant to be ingested for the purpose of obtaining a beneficial effect for a health use such as the action.

The above-mentioned health functional foods may contain usual food additives, and the above food additives may be added to the standards and standards of the relevant items in accordance with the General Rules for Food Additives approved by the Food and Drug Administration and the General Test Methods, It can be judged whether it is suitable or not.

The food additives described in the above-mentioned Food Additives Code include chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as persimmon extract, licorice extract, crystalline cellulose, high- A preservative formulation, a tar coloring agent, and the like.

The health functional food may be variously used for prevention or improvement of alcoholic liver diseases or food and beverages for hangover, etc. For example, various foods, beverages, gums, tea, vitamin complex, health functional auxiliary food, And the like.

The health functional food may be manufactured and processed into any one form selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings for the purpose of preventing or improving alcoholic liver disease and hangover.

Specifically, the health functional food in the form of tablets can be prepared by granulating the compound, excipient, binder, disintegrant and other additives in a conventional manner, compression-molding the mixture with a lubricant or the like, . The health functional food of the tablet form may contain a mating agent and the like if necessary, and may be sieved to a suitable skin care agent if necessary.

The hard capsule of the above-mentioned capsule type health functional food can be prepared by filling a normal hard capsule with a mixture of the above compound and an additive such as an excipient, or a granular product thereof or a granular product obtained by soaking the product. And the like can be filled in a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The above-mentioned ring-form health functional food can be prepared by molding a mixture of the above compound, excipient, binder, disintegrant and the like by an appropriate method. If necessary, the preparation may be mixed with white sugar or other suitable skin care agent or a starch, talc or a suitable substance It is possible that the

The granular health functional food may be prepared by granulating a mixture of the above compound, excipient, binder, disintegrant and the like by a suitable method, and if necessary, may contain a flavoring agent, a mating agent and the like.

Further, the definitions of the above excipients, binders, disintegrants, lubricants, mating agents, flavoring agents and the like are described in documents known in the art, and the functions and the like may be the same or similar.

It will be apparent to those skilled in the art that various changes and modifications may be made thereto without departing from the spirit and scope of the present invention as set forth in the appended claims. It is encompassed in the technical idea of.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Production Example: Preparation of Herbal Extract

After washing with water, dust, sane and dermis, they were thoroughly dried at room temperature and pulverized to obtain 500 g of pulverized product.

Each of the pulverized materials was subjected to reflux extraction twice with 4 L of 95% (v / v) ethanol for 2 hours.

The extract was filtered, concentrated under reduced pressure, and lyophilized to obtain a solid content of each extract.

Examples and Comparative Examples

In order to verify the anti-obesity effect of the obtained extracts, a mixed sample was prepared as shown in Table 1 below to set Examples and Comparative Examples.

[Content (parts by weight)] division Example Comparative Example One 2 3 One 2 3 4 5 6 Garlic extract 7 7 7 10 - - 7 3 7 Manure extract 2.5 2.5 2.5 - 10 - - - 1.25 L-asprtic acid 3 3 3 - - 10 3 7 3 Sine extract - 0.2 0.2 - - - - - - Dermis extract - - 0.7 - - - - - -

EXPERIMENTAL EXAMPLE 1 Evaluation of Blood Alcohol Concentration Effect

Animal experiments were conducted to verify the effect of reducing the blood alcohol concentration on the samples of the Examples and Comparative Examples.

A male ICR-type 6-week-old mouse purchased from Sam Taco Co., Ltd. (Osan, Gyeonggi-do) was used as an experimental animal. The experimental animals were fed at 23 ± 1 ℃ and 12 hours / day under controlled environmental conditions, and feed and water were supplied freely.

After a week of adaptation period, 8 animals per group were selected.

Samples of each of the examples and comparative examples were orally administered to the experimental animals of each group at a dose of 2 g / kg, and 30 minutes later, 40% alcohol was orally administered at a dose of 0.1 mL per 1 g.

One hour after the administration of alcohol, blood was collected from the heart of the experimental animals, centrifuged at 3000 rpm for 10 minutes, and serum was taken.

Serum was assayed using the NAD-ADH multiple test kit (Sigma, USA).

Alcohol was decomposed into acetaldehyde and NADH by alcohol dehydrogenase when NAD was present. NADH was measured at 340 nm in the ultraviolet region (Table 2).

The percent inhibition of blood alcohol concentration in Examples and Comparative Examples is shown in Table 2 below, when the percentage of reduced NADH was measured by the blood alcohol concentration inhibition rate as compared to the control group to which no sample was added.

[% of control] division Inhibition rate % ) Example 1 23.20 Example 2 24.10 Example 3 24.95 Comparative Example 1 10.04 Comparative Example 2 6.55 Comparative Example 3 13.15 Comparative Example 4 13.35 Comparative Example 5 10.42 Comparative Example 6 13.87

The higher the percent reduction of NADH, the lower the amount of alcohol in the blood. Therefore, the higher the inhibition rate of the blood alcohol level, the better the prevention and improvement of alcoholic liver disease and the better the hangover resolution effect.

Referring to Table 2, the blood alcohol concentration inhibition rate of the examples was measured to be higher than that of the comparative examples. Especially, the alcohol concentration inhibiting rate of the Example was higher than that of Comparative Example 3, which is an L-aspartic acid capable of detoxifying alcohol.

Experimental Example 2: Evaluation of liver protection against alcohol-induced hepatotoxicity

Animal experiments were conducted to verify the liver protective effect against the samples of the examples.

A male SD (Sprague-Dawley) 6-week-old mouse purchased from Sam Taco Co., Ltd. (Osan, Gyeonggi-do) was used as an experimental animal. The experimental animals were fed at 23 ± 1 ℃ and 12 hours / day under controlled environmental conditions, and feed and water were supplied freely.

After a week of adaptation period, 8 animals per group were selected.

To confirm that the samples of the examples were effective in inhibiting and treating alcoholic liver disease, the samples were divided into a normal group not consuming alcohol, a comparative group consuming only alcohol, and an alcohol and each sample.

Alcohol was given daily at 10 g / kg for the first week, 12 g / kg for the second, 14 g / kg for the third and 16 g / kg for the fourth week.

Samples were orally administered at a dose of 1 g / kg for 4 weeks. Silymarin 50 mg / kg was used as a control.

The weight of the mice was measured before and after the experiment, and the liver weights of the mice were measured after completion of the experiment (Table 3).

Blood was collected from the heart of the experimental animals, centrifuged at 3000 rpm for 10 minutes, and serum was taken.

Glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), triglycerides (TG), total cholesterol (TC) and high density lipoprotein (HDL) (Prime, BPC biosed, Italy) using a blood auto analyzer (Table 4).

Lipid peroxidation of liver tissue was measured by absorbance of 535 nm by measuring the color of malondialdehyde (MDA) (Fig. 1).

division Weight before experiment
(g) Weight after experiment
(g) Weight increase
(g) Liver weight
(g) Normal group 230.3 ± 12.5 340.4 ± 12.4 113.3 ± 12.5 13.8 ± 3.8 Comparative group 219.5 ± 9.3 321.3 ± 11.9 102.9 ± 11.2 17.5 ± 2.7 ^# Example 1 215.9 ± 15.7 327.8 ± 15.2 114.2 ± 14.6 15.9 ± 2.9 Example 2 226.8 ± 12.6 332.5 + - 16.4 105.7 ± 16.6 15.5 ± 3.1 Example 3 225.7 + - 10.4 337.6 ± 15.7 111.9 ± 14.5 15.0 ± 3.5 Control group 224.6 ± 12.5 338.4 ± 17.2 116.5 ± 15.4 14.1 ± 3.1

Results were expressed as means ± standard error (SE) and one-way ANOVA test was performed to determine significance. Dunnett's test was used for post-test, and statistical significance was defined as p <0.05.

In the experimental results, the significance level of P <0.05 was indicated as #.

As shown in Table 3, the weight of the experimental group was almost unchanged, but the weight of the liver of the comparative group was significantly increased, and the hepatic weight of the experimental group was similar to that of the normal group and the control group.

division GOT
(U / L) GPT
(U / L) ALT
(U / L) TC
(mg / dl) TG
(mg / dl) Normal group 135.8 ± 13.5 25.8 ± 2.4 355.3 ± 24.9 32.7 ± 3.9 109.8 ± 9.9 Example 1 397.5 ± 37.7 *** 264.2 ± 20.9 ** 565.3 ± 28.3 * 51.7 ± 5.4 *** 213.0 ± 28.6 *** Example 2 379.7 ± 30.5 *** 260.8 ± 26.8 *** 551.0 ± 36.4 * 49.8 ± 5.7 *** 198.2 ± 32.4 *** Example 3 368.5 ± 29.9 *** 249.8 ± 29.2 *** 540.0 + - 24.5 * 47.7 ± 7.2 *** 185.7 ± 30.4 *** Comparative group 765.3 + - 107.0 ^### 838.3 ± 44.9 ^### 664.8 + - 42.1 ^### 90.3 ± 4.4 ^### 394.0 ± 23.9 ^### Control group 346.7 ± 10.22 *** 233.3 ± 27.2 *** 419.7 ± 21.8 *** 38.8 ± 6.0 *** 136.3 ± 22.9 ***

Results were expressed as means ± standard error (SE) and one-way ANOVA test was performed to determine significance. Dunnett's test was used for post-test, and statistical significance was defined as p <0.05.

In the experimental results, when the significance level compared to the normal group was P <0.001, they were represented as ###, and when the significance level was compared with the comparison group, p <0.05, p <0.001, *, ***.

The concentration of GOT, GPT, ALT, TC, and TG in the blood was increased in the comparative group compared to the normal group, but the concentration of GOT, GPT, ALT, TC and TG in the blood was greatly decreased in the examples.

Referring to FIG. 1, the MDA level was increased in the comparative group compared to the normal group in alcohol-induced liver damage, but the MDA level was decreased in the examples.

These results suggest that the composition can provide liver protection against liver damage caused by alcohol.

Experimental Example 3: Evaluation of the effect of reducing the blood alcohol concentration in the clinical trial

Subjects were recruited to verify the effect of reducing the blood alcohol level in the clinical sample.

The blood alcohol concentration was measured after drinking the sample of the example, and the blood alcohol concentration was measured after drinking without taking the composition.

Alcohol concentration was 1.2 g / kg, and 360 mL of 20% alcohol was added to 60 mL of water for 6 minutes to maximize blood alcohol concentration in a short time.

The subjects measured blood alcohol levels at 15, 30, 60, 90, 120, 180, and 240 minutes after drinking with an alcohol meter (CA-2000) (FIG.

Referring to FIG. 2, the blood alcohol concentration of the subjects who drank the samples of the example was decreased by 19% or more as compared with the control group of the alcohol alone. Blood alcohol levels were reduced by about 10 to 17% in the experimental group taking the example up to 180 minutes compared to the control.

These results suggest that the composition has an excellent blood alcoholic concentration inhibitory effect in the human body and an excellent liver protecting effect.

In addition, the above results suggest that the composition can be used as a functional food or medicine such as hangover-free beverage for the purpose of inhibiting blood alcohol concentration and protecting liver.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (11)

Herbal extracts composed of garlic extract and guinea extract; And
L-aspartic acid as an active ingredient. The method according to claim 1,
Wherein the complex herbal medicine extract comprises 5 to 9 parts by weight of a Galangal extract and 2 to 3 parts by weight of a guinea extract. 3. The method of claim 2,
Wherein the complex herbal medicine extract further comprises a sine extract or a dermis extract. 3. The method of claim 2,
Wherein the complex herbal extract further comprises 0.1 to 0.3 parts by weight of a sine extract. 3. The method of claim 2,
Wherein the complex herbal extract further comprises 0.5 to 0.9 parts by weight of a dermis extract. The method according to claim 1,
2 to 4 parts by weight of said L-aspartic acid. A pharmaceutical composition for the prevention and treatment of alcoholic liver diseases comprising the composition of any one of claims 1 to 6. 8. The method of claim 7,
Wherein the alcoholic liver disease is at least one selected from the group consisting of alcoholic hepatitis, alcoholic fatty liver, liver cirrhosis and liver cancer. A pharmaceutical composition for hangover use comprising the composition of any one of claims 1 to 6. A health functional food for preventing and improving an alcoholic liver disease comprising the composition of any one of claims 1 to 6. A health functional food for hangover habit comprising the composition of any one of claims 1 to 6.

Images