KR20190007721A - CARTRIDGE for cell culture - Google Patents

Abstract

The present invention relates to a cartridge for culturing cells, comprising a cell culture support including a fabric, a first support disposed on a bottom surface of the cell culture support, and a second support disposed on a top surface of the cell culture support, wherein the first support and the second support each include an edge portion and a center portion. In particular, the cartridge permits the cell culture supports to be conveniently attached and detached and permits the cell culture supports be fixed, thereby providing a stable environment for cells being cultured, while increasing cell culture efficiency. In addition, the cartridge can prevent the cell culture supports from shaking while the cells are being cultured, thus improving the cell culture efficiency. Also, the cartridge can prevent the supports from moving even when cell culture broth is being dispersed in the cell culture supports, and can provide mechanical, physical, and chemical stimuli uniformly to all cells being cultured on all portions of the supports, thus uniformly proliferating the cells. Further, the cartridge of the present invention enables culturing of cells in high density, which is suitable for use in fields requiring a large-batch production of cells, and can culture cells having a desired shape suitable for a target test and experiment without causing damage to the cells during a process of separating the cell culture supports.

Description

[0001] CARTRIDGE for cell culture [

The present invention relates to a cell culture cartridge, and more particularly, to a cell culture cartridge which is capable of providing a stable cell culture environment with ease of desorption of a cell culture support and is advantageous for high-density cell culture.

Recently, as the use of cultured cells for disease treatment is expanded, interest and research on cell culture are increasing. Cell culture is a technique for extracting cells from a living body and culturing them out of the living body. The cultured cells are differentiated into various tissues of the body such as skin, organs, nerves, etc. and transplanted into the human body before transplantation into the human body. And can be used for treating various diseases.

This field of tissue culture is related to cell culture and it is a multidisciplinary study applying existing scientific fields such as cytology, life sciences, engineering, medicine, etc., and the correlation between the structure and function of living tissue And new fusion techniques are being studied to replace damaged tissues or organs with normal tissues and regenerate them.

On the other hand, in the field of regenerative medicine, a study is carried out to prepare a cell culture support and a cell culture unit so that cells collected from a patient can be cultured in vitro, and the cells cultured in the cell culture support are transplanted in vivo to improve the medical effect have.

In order to cultivate the cells through such a cell incubator, the desired cell-cultured support can be cultured in a cell incubator by injecting various cell cultures in consideration of the size and type of the cells.

However, cell culture in a conventional cell culture incubator has the following problems.

First, since the cell culture support is not immobilized in the cell culture medium, the cell may be killed even when the cell culture support is finely shaken or subjected to a small impact, and the survival rate of the cell is deteriorated.

Second, in order to transplant the cultured cells, it is necessary to separate them from the cell culture medium by physicochemical mechanism to the cell culture support having the cultured cells attached thereto. In this case, the indirect destruction of the cell culture support or direct The physiological and chemical action may damage the cells and decrease the survival rate of the cells.

Third, when the cell culture support is immobilized to the cell culture medium through the adhesive film or the like, the support may be damaged during the separation of the cell culture support, which is disadvantageous in view of the reusability of the support.

Fourth, the conventional cell culture incubator has a problem in that it is difficult to cultivate high-density cells that have the same microenvironment while having a plurality of cell culture supports, which is not suitable in a field where a large amount of cell culture is required as in the field of regenerative medicine.

Accordingly, the present inventors have found that a cell culture support can be immobilized on a cell culture incubator, thereby providing a stable cell culture environment, facilitating desorption of a cell culture support from a cell culture incubator, preventing cell or support damage during the separation process, The present invention has been completed based on studies on cartridges for cell culture which are advantageous for cell culture of cells.

Japanese Patent Application Laid-Open No. 10-2014-0048733

Disclosure of the Invention The present invention has been made in view of the above problems, and provides a cell culture cartridge capable of easily detaching a cell culture support and fixing a cell culture support to provide a stable cell culture environment, thereby improving a cell culture rate There is a purpose.

In addition, the present invention can prevent shaking of the supporter even when the cell culture medium is dispersed in the cell culture supporter, and it is possible to uniformly multiply the cells by imparting the same mechanical, physical, and chemical stimulus to each cultured cell regardless of the supporter Lt; RTI ID = 0.0 > incubator. ≪ / RTI >

It is another object of the present invention to provide a cell culture cartridge capable of high-density cell culture so as to be suitable for a field in which a large number of cells must be cultured.

Another object of the present invention is to provide a cell incubator capable of culturing a cell having a shape suitable for a desired test and experiment without damaging the cell in the process of separating the cell culture support.

In order to solve the above-described problems, the present invention provides a cell culture support comprising a cell culture support, a first support portion disposed on a lower surface of the cell culture support, and a second support portion disposed on an upper surface of the cell culture support, Each of the support portion and the second support portion includes a rim portion and a center portion.

According to an embodiment of the present invention, at least one of the first support portion and the second support portion includes at least one protrusion, and the rim of the other one of the first support portion and the second support portion As shown in FIG.

In addition, the central portion may include a frame portion that performs a supporting function and a mesh portion through which a cell culture liquid is permeated.

In addition, the surface of the mesh portion may include a cell adhesion inhibitor.

In addition, the fabric may be woven into a yarn of at least one of a yarn spun yarn, a filament yarn, and a slitting yarn.

In addition, the yarn may further include a cell culture improving material.

In addition, the cell culture enhancing substance may be a physiologically active ingredient that induces at least one of cell adhesion, migration, growth, proliferation, and differentiation.

In addition, the physiologically active component may be selected from the group consisting of monoamines, amino acids, peptides, saccharides, lipids, proteins, glucoproteins, glucolipids, proteoglycans, mucopolysaccharides, ) And one or more of the cells.

In addition, the yarn may be selected from the group consisting of polystyrene (PS), polyethylene terephthalate (PET), polyether sulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane Amide, polyalkylene, poly (alkylene oxide), poly (amino acids), poly (allylamines), polyphosphazene and polyethylene oxide-polypropylene oxide Block copolymers, or at least one non-biodegradable fiber-forming component selected from the group consisting of polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly (L- lactide, lactide), PLGA (poly (DL-lactide-co-glycolide)), polylactic acid and polyvinyl alcohol. There .

The present invention also provides a cell incubator comprising a plurality of the cell culture cartridges stacked inside.

In addition, the present invention provides a cell culture cartridge array in which a plurality of cell culture cartridges are fixed with a predetermined spacing therebetween.

The present invention also provides a cell incubator comprising the cell culture cartridge array.

According to the present invention, the cell culture support can be easily desorbed and the cell culture support can be fixed, thereby providing a stable cell culture environment and improving the cell culture rate.

In addition, it is possible to prevent shaking of the cell culture supporter during the culturing of the cells, thereby improving cell culture efficiency.

In addition, even if the cell culture medium is dispersed in the cell culture support, it is possible to prevent shaking of the support, and uniform cell growth can be achieved by imparting the same mechanical, physical and chemical stimulation to each cell to be cultured regardless of the region of the support.

The present invention enables a high-density cell culture to be suitable for a field where a large number of cells must be cultured.

The present invention can cultivate a cell having a shape suitable for a desired test and experiment without damaging the cell in the process of separating the cell culture support.

1 is an exploded perspective view showing a cell culture cartridge according to the present invention,
FIG. 2 is a perspective view showing a first supporting part of the cell culture cartridge according to the present invention,
3 is a perspective view showing a second support part of the cell culture cartridge according to the present invention,
4 is a perspective view and a sectional enlarged view showing a cell culture support according to the present invention,
FIG. 5 is a perspective view showing a state where first and second supporting portions of a cell culture cartridge according to the present invention are combined with a cell culture support,
6 is a cross-sectional view taken along the line I-I 'shown in Fig. 5,
7 is a perspective view showing a cell incubator including the cell culture cartridge according to the present invention, and
8 is a perspective view showing another cell incubator including the cell culture cartridge according to the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same reference numerals are assigned to the same or similar components throughout the specification.

The cells that can be cultured industrially are classified into cell line cells obtained from the primary culture cells and stem cells which are undifferentiated cells capable of differentiating into various types of cells in the embryo or adult body . Recently, it has been reported that stem cells require much more delicate manipulation than cell line cells. In other words, the cell may be killed even when the cell culture support 300 has been cultured in the course of cell culture. However, in order to solve this problem, the cell culture support 300 is generally placed in a cell incubator So as to minimize the influence of external shaking or impact on the cells.

However, the cells cultured in the cell culture support 300 fixed on the floor tends to grow and grow only two-dimensionally, making it difficult to produce a three-dimensional cell cluster. In addition, when there is a cell that propagates and grows inside the cell culture supporter, the cell culture solution is hardly uniformly dispersed into the cell culture support 300, and the cell culture solution that penetrates into the supporter is a cell There is a problem that the culture solution and the circulation are not smoothly performed, which causes the uniformity of the cultured cells to be lowered and the cell culture efficiency to be significantly lowered.

In order to solve the above problems, when the cell culture support 300 is fixed on the side wall of the cell culture apparatus in a form spaced apart from the bottom of the cell culture chamber, the cells attached to the upper and lower surfaces of the cell culture support 300 The culture liquid can be evenly dispersed. However, in order to fix the cell culture support 300 to the inside of the cell culture incubator, an additional structure such as an adhesive film is required, thereby causing a problem of contamination of the cell culture solution, and it is easy to recover the cell culture support 300 from the cell culture I do not. In other words, in the process of recovering the cell culture support 300, an excessive external force may be applied to the cell culture support 300 to kill the cells or damage the cell culture support 300 itself.

Further, in a specific field requiring large cell culturing such as pharmaceuticals, cosmetics, and regenerative medicine, a high density cell culture environment should be provided while keeping uniform physical properties such as the shape and size of each cultured cell. In addition, there is a need for a cell culture substrate capable of predicting or controlling cell-living organisms to suit the purpose of the cells to be cultured.

Accordingly, the present invention provides a cell culture cartridge that fixes the cell culture support on the upper and lower surfaces of the cell culture support 300, thereby assuring the stability of the cell culture environment and accommodating the cell culture support 300 individually It is possible to control each of them, and it is possible to cultivate a large number of cells and to improve the uniformity of each cell culture environment.

1 to 3, the cell culture cartridge 10 according to the present invention includes a first support 100, a second support 200, and a cell culture support 300. 1 to 3 show a circular cell culture cartridge 10, the cell culture cartridge 10 may be any shape as long as it can accommodate the cell culture support 300, Or the size and the type of the cell culture support 300. In addition,

The first support part 100 is disposed on the lower surface of the cell culture support 300 to support the cell culture support 300. The first support portion 100 includes a center portion 120 and a rim portion 110 surrounding the side surface of the central portion 120 to maintain the overall frame of the first support portion 100.

The rim 110 maintains the shape of the cell culture cartridge 10 according to the present invention, and a part of the upper part of the cartridge 110 can be in contact with the cell culture support 130. In this case, the cell culture support body 130 can be pressed and fixed together with the second support part 200, which will be described later, from above and below. In this case, the cell adhesion inhibiting substance may be coated on the surface of the first support part 100 in order to prevent the cells from being attached to the surface of the first support part 100 or to prevent damage during the separation process. Will be described later.

The material of the rim 110 is not particularly limited, but known materials capable of preventing deformation due to physical and chemical stimulation and deterioration of mechanical properties during cell culture can be used. For example, the rim portion 110 may be formed of an inorganic material such as metal, glass, ceramics or silicon, an elastomer, a plastic (for example, a polyester resin, a polyethylene resin, a polypropylene resin, an ABS resin, a nylon, An organic material typified by a resin, a polycarbonate resin, a polyurethane resin, a methylpentene resin, a phenol resin, a melamine resin, an epoxy resin, and a vinyl chloride resin) can be used. But the shape thereof is not limited. For example, it may have a flat shape such as a flat plate, a flat film, a film, or a three-dimensional shape. When a film is used, its thickness is not particularly limited, but it may have a range of usually from 0.1 to 1000 mu m.

In addition, the rim 110 may include at least one receiving portion 111 through which the protruding portion 211 of the second supporting portion 200 described below may be inserted. The structure in which the projecting portion 211 is drawn into the receiving portion 111 will be described later.

The center portion 120 surrounded by the rim portion 110 and contacting the cell culture support body 130 may include a frame portion 121 and a mesh portion 123.

The frame part 121 is connected at both inner ends of the rim 110 and is connected to the cell culture support 300 while forming the overall frame of the cell culture cartridge 10 together with the rim 110. [ Support function. Accordingly, the frame 121 may be made of the same material as the rim 110, but is not limited thereto. In addition, although the frame part 121 is shown as being composed of the frame part 121 in the horizontal direction and the vertical direction, it may be formed in various forms such as a concentric circle shape and a polygonal shape in consideration of the type, size and shape of a target cell. Likewise, the number of the frame portions 121 is not limited, and can be modified to such an extent that the transmittance of the cell culture liquid is not reduced.

The rim 110 and the frame 121 may adhere to the cells cultured in the cell culture support 300. In this case, the cells may be attached to each other and die, and the cell culture support 300 Peeling is not easy in the process of separating and recovering from the first supporter 100, and the cell viability and the cell recoverability may be lowered at the same time.

Therefore, the cell adhesion inhibiting substance may be coated on the surface of at least one of the rim 110 and the frame 121. For example, a coating of an organic compound having a carbon-oxygen bond may be formed on the surface of the frame portion 121 to prevent adhesion to cells cultured in the cell culture support 300. The material of the cell adhesion inhibiting substance is not particularly limited as long as it is a material capable of forming a film of an organic compound having a carbon-oxygen bond. Specifically, it is possible to use an inorganic material such as metal, glass, ceramics and silicone, an inorganic material such as an elastomer, a plastic (for example, a polyester resin, a polyethylene resin, a polypropylene resin, an ABS resin, a nylon, an acrylic resin, a fluorine resin, a polycarbonate resin, Resin, methylpentene resin, phenol resin, melamine resin, epoxy resin, vinyl chloride resin).

The mesh portion 123 may be formed in a space surrounded by the frame portion 121 and the frame portion 110 or one frame portion 121 and the other frame portion 121. The mesh part 123 may be provided on the first supporting part 100 so as to prevent the cell from being detached from the cell supporting support 300 so that the mesh part 123 directly contacts the cell supporting support 300 or is separated from the supporting part 300 by a predetermined distance.

That is, the cells cultured on the cell culture support 300 may be desorbed by the permeation pressure of the cell culture medium and / or the minute external force applied to the cell culture support 300 during the cell culture process. It is possible to prevent cell culture support 300 from shaking or cell detachment by protecting cell culture support 300 from pressure or external force, and in the case of relatively large cells, it is possible to prevent desorption directly to the outside. In addition, during the process of separating the cell culture support 300, direct external stimulation to the cells can be prevented.

The material of the mesh portion 123 is not particularly limited, but may be composed of a material derived from a living body preferably having affinity for cells in terms of contact with the cells cultured in the mesh portion 123. Specific examples of the material include a polymer such as various collagen, peptide hydrogel, laminin, chondrogenetin, glycosaminoglycan, hyaluronic acid, proteoglycan, extracellular matrix component protein, gelatin, agarose, oligonucleic acid, A gel with a compound.

In addition, a material mimicking an artificially synthesized bio-derived material may be used instead of being extracted from a living body. For example, various artificial peptides and derivatives thereof, artificial oligopeptides and derivatives thereof, artificial polypeptides and derivatives thereof, artificial polysaccharides and derivatives thereof, and the like.

Further, the mesh part 123 may be attached or embedded with a catalytic substance such as an inorganic catalyst and an organic catalyst, or a physiologically active ingredient which is useful for cell culture. The organic catalyst, the inorganic catalyst, and the physiologically active components may be physically or chemically adsorbed by using a simple adsorption method, a method using ionic bonds between materials, or a chemical bonding method using a functional group of a substance. Polydopamine, polynorepinephrine An organic catalyst, an inorganic catalyst, or a physiologically active component may be attached to or embedded in the mesh portion 123 through various methods such as a method using an adhesive material based on a catechol group,

2 and 3, the mesh unit 123 according to an embodiment of the present invention includes a plurality of first mesh lines 123a and a plurality of second mesh lines 123b arranged to intersect with each other, . A plurality of porous holes 123c may be formed by intersecting the first and second mesh lines 123a and 123b. At this time, the thicknesses of the first and second mesh lines 123a and 123b are adjusted so that the cell culture liquid can smoothly permeate the porous holes 123c and provide a uniform culture environment to the cell culture support 300 Can be adjusted.

That is, the sizes of the porous holes 123c formed from the mesh lines can be adjusted corresponding to the viscosity and the permeation rate of the cell culture liquid, so that a uniform micro environment can be provided to each cell cultured in the cell culture support 300 And the porous hole 123c, the upper part of the cell is not covered and the restriction of the directionality of cell proliferation can be alleviated

In the mesh part 123 according to the embodiment of the present invention, the area of the porous hole 123c may be in the range of 5 to 50 탆 so that the cell culture liquid can be smoothly permeated. If the area of the porous hole 123c exceeds 50 占 퐉, there is a problem that the cell culture support body is damaged by the permeation pressure of the cell culture liquid, or a uniform culture liquid can not be supplied to the cultured cells. If the area of the porous hole 123c is less than 5 占 퐉, the cell culture fluid may not permeate smoothly, and the culture medium may be accumulated in the mesh portion and the frame portion, which may result in uneven cell culture environment.

Next, the second support portion 200 will be described. However, the description of the configuration common to the above-described first support portion 100 will be omitted.

The second support part 200 may be disposed on the upper surface of the cell culture support 300 to fix the cell culture support 300 together with the first support part 100. The second support part 200 includes a frame part 210 for holding the frame of the second support part 200 and a center part 220 for covering the outer side of the frame part 210.

The rim portion 210 may include a protrusion 211 that can be received in the receiving portion 111 of the first support portion 100. As shown in the drawing, the projecting portion 211 may be differentiated from the rim 210 and may have a latching jaw at an end thereof.

5, the projecting portion 211 is inserted into the receiving portion 111 of the first supporting portion 100 and is engaged with the first supporting portion 100 to fix the first supporting portion 100 and the second supporting portion 200 in a fitting manner And can not be released even with a constant external force, thereby providing a more stable cell culture environment. Further, by applying pressure to the fastening portion of the protrusion 211, the first support portion 100 and the second support portion 200 can be easily separated from each other. It should be noted that the receptacle 110 shown in the figure is for convenience of description and that the first and second supports 100 and 200 can be stably fixed and easily separated, It is possible to deform it into the protruding portion 211.

Also, as described above, the positions of the receiving portion 111 and the protruding portion 211 can be changed. That is, one of the rim portions 110 and 210 of the first supporting portion 100 and the second supporting portion 200 includes at least one protruding portion 211, and the first supporting portion 100 and the second supporting portion 200 The protruding portion 211 may be provided with a receiving portion 111 through which the protruding portion 211 is inserted.

Next, the cell culture support 300 will be described.

The cell culture support 300 according to the present invention includes a fabric, and may further include at least one of a knitted fabric and a nonwoven fabric. However, when the cell culture support 300 comprises only a fabric, the permeability of the cell culture fluid may be improved due to the regular arrangement of the fibers formed of the weft and the warp fibers.

For example, as shown in FIG. 4, when the cell culture support 300 is composed of a fabric, the fibers 310 included in the fabric have longitudinal and transverse directions, and the specific tissue may be plain weave, And the density of warp and weft yarns is not particularly limited.

According to one embodiment of the present invention, when the cell culture support 300 is composed of a fabric, the yarn 310 constituting the fabric may be composed of one or a plurality of monosa, , Filament yarn, slitting yarn, or electrospun nanofiber.

If monosaccharides are spun yarns or filament yarns, the fineness may be between 0.1 and 1,000 denier. However, the present invention is not limited thereto and may be modified to suit the kind, size, shape and size of the cell cluster to be cultured.

The spinning yarn may also be produced through a raw cotton by a known method. Further, the filament yarn may be one produced by spinning by a known method, and the spinning may be a known spinning method such as chemical spinning or electrospinning.

In addition, the slitting yarn may be produced by cutting a sheet-like fiber aggregate, a fabric or the like so as to have a predetermined width. Preferably, the slitting yarn may be a mono-yarn produced by cutting a sheet-like fibrous web having a three-dimensional network structure to have a predetermined width. At this time, the fibrous web may be pressed at a constant pressure to improve the ease of the slitting process and increase the strength of the slitting yarn.

For example, the slitting yarn may be a mono-sintered fiber web having a basis weight of 0.1 to 100 g / m < 2 > and having a width of 0.1 to 30 mm. If the width is less than 0.1 mm, it is not easy to cut and there is a problem that it can be easily broken by the tensile force and the rotational force applied in the case of flammability. Further, when the width is more than 30 mm, there is a problem that nonuniform twisting may occur at the time of twisting.

In addition, the electrospun nanofibers can be folded and arranged / laminated a plurality of times without any orientation, thereby forming a three-dimensional network structure. Or a plurality of nanofibers, each of the nanofibers can be independently arranged in a folded and / or non-fixed length direction to form a three-dimensional network structure. At this time, adhesion or fusing may occur between the different surfaces and / or the surfaces of the different nanofibers in the one-stranded nanofiber, thereby making the three-dimensional network structure more structurally complicated, Can move / propagate into pores formed by the structure, and may be advantageous for culturing cells with a cell aggregate having a three-dimensional shape / structure. In order to increase the proliferation rate and the survival rate of the cells cultured in the inside / outside of the support, it is important to supply the nutrients necessary for cell proliferation. The channel of the culture solution containing the nutrients of the nanofibers of the three- It is possible to easily supply nutrients to the cells located inside the support to prevent cell death and improve cell proliferation.

The mono yarns constituting the yarn 310 described above may be embodied as a known fiber forming component that can be produced in the form of fibers, and may be realized by selecting a suitable material according to the mono yarn type, The material can be selected in accordance with a particular purpose, so that the present invention is not particularly limited thereto. The fiber forming component may include a natural fiber component such as a cellulose component such as cotton, hemp, etc., a protein component such as wool, silk, or a mineral component. Or the fiber forming component may be a component of known artificial fibers.

On the other hand, the fiber forming component may be selected from the group consisting of polystyrene (PS), polyethylene terephthalate (PET), polyether sulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), polyamide, polyalkylene, poly (alkylene oxide), poly (amino acids), poly (allylamines), polyphosphazene and polyethylene Oxide-polypropylene oxide block copolymer, or at least one non-biodegradable component selected from the group consisting of polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly (L a biodegradable component selected from the group consisting of poly (lactide-co-glycolide), poly (lactide-co-glycolide), polylactic acid and polyvinyl alcohol .

Next, the average cell culture liquid permeability of the cell culture support 300 according to the present invention may be 10 to 10,000 ml / mm ² · min · bar for a cell culture having a viscosity of 0.1 to 100 cps. The permeation amount of the cell culture fluid circulating through the cell culture support 300 must satisfy the minimum amount of permeation to the cell culture medium because it can affect various growth and differentiation of the cells cultured in the cell culture support 300. However, when the amount of permeation is excessive, the cultured cells are desorbed to decrease the density of the cells, or excessive pressure may be applied to the cell culture support 300, so that the support may collapse. Therefore, the cell culture medium having a viscosity of 0.1 to 100 cps may have a range of ^{10 ~ 10,000 ㎖ / mm 2 ·} min · bar. In addition, when the cell culture liquid circulates in the cell culture apparatus 100 during cell culture and the culture solution is supplied to the cells or the culture solution is continuously permeated, the cell culture support 300 can not withstand the permeation pressure of the cell culture liquid, Or may be damaged. Therefore, it is necessary to satisfy a certain level of permeability.

Meanwhile, the cell culture medium may cause growth and reaction of cells to be cultured. When the frictional force due to the flow of the cell culture fluid circulating in the cell culture support 300 is excessive, the cultured cells recognize it as a stimulus Physiological reactions can be induced. Therefore, it is necessary to control the minute flow rate and flow rate of the cell culture fluid in order to suppress the undesired side reaction. In particular, the initial adhesion time at which the cells are attached to the cell culture support 300 should be controlled by controlling the flow rate and flow rate of the cell culture liquid to suppress the side reactions. For example, considering the size and type of cells to be cultured and the physical properties (basis weight, porosity, etc.) of the cell culture support 300 between 3 and 6 hours when the cells are attached to the cell culture support 300, The flow rate and flow rate should be adjusted appropriately.

In addition, the average pore size of the cell culture support 300 according to the present invention may be 10 to 100 탆. When the cell culture support 300 is arranged as a fabric in a space between the yarns 310 formed by arranging the yarns 310 constituting the cell culture support 300 as light and weft, All can be uniformly formed. The average pore size of the pores is closely related to the amount of permeation of the cell culture liquid. If the average pore size of the pores is less than 10 mu m, the permeability of the cell culture liquid decreases and the circulation of the cell culture liquid is not smooth. Or excessive pressure of the cell culture support 300 may be applied to the cell culture support 300 to bend or cut. When the average pore size of the pores is more than 100 mu m, the permeation amount of the cell culture liquid increases and the mechanical strength of the cell culture support 300 is lowered, which may lower the stability of the cell culture.

Also, the porosity of the cell culture support 300 according to the present invention may be 1 to 20%. The porosity represents the area of the pores of the whole yarn 310 constituting the cell culture support 300 and has a close relationship with the average pore size of the pores and the permeation amount of the cell culture liquid. If the porosity of the cell culture support is less than 1%, the permeability of the cell culture solution is lowered and the circulation of the cell culture solution is not smooth, so that the uniformity of the cell culture is lowered or excessive pressure of the cell culture support 300 is applied, (300) can be bent or cut. In addition, if the porosity exceeds 20%, the permeation amount of the cell culture fluid increases and the mechanical strength of the cell culture support 300 may be lowered, resulting in deterioration of cell culture stability.

The basis weight of the cell culture support 300 according to the present invention may be 10 to 100 g / m 2. If the basis weight of the cell culture support 300 is less than 10 g / m < 2 >, the cell culture support 300 can not withstand the pressure of the cell culture solution and can be mechanically damaged such as bending. If the basis weight of the cell culture support 300 If it exceeds 100 g / m < 2 >, the opacity of the cell culture support 300 is too high to observe cells easily, and problems may arise when the cells are removed or moved during the movement of the cultured cells .

As described above, the cell culture support 300 according to the present invention has a cell culture fluid permeation amount higher than a certain level, thereby circulating the cell culture solution evenly, thereby providing a uniform microenvironment of the cells attached to the upper and lower surfaces of the support, Uniformity can be improved.

In addition, the yarn 310 constituting the cell culture support 300 may further include at least one of an adhesive component and a physiologically active component as a cell growth improving substance in addition to a fiber forming component. At this time, the inside of the yarn is not provided with the adhesive component or the physiologically active component, and the physiologically active component can be fixed to a part of the outer surface of the yarn through the adhesive component fixed to one area of the outer surface. Or a physiologically active component may be provided so as to completely cover the adhesive component provided on the entire outer surface of the yarn.

Recently, cell culture technology has been studied in an attempt to realize the intercellular environment in vivo in vitro . In order to construct the cell culture environment similar to the cell environment in the body, It is in the trend to incorporate various components into culture solutions when cultivated in vitro . However, when a substance capable of promoting cell culture is added to a culture solution, there is a limit to continuously expose the substance to the cultured cells. For the continuous exposure, the content of the substance should be increased in the culture solution. And there is a problem in the propagation efficiency.

 Accordingly, the surface of the yarn 310 according to an embodiment of the present invention may be provided on the support with the adhesive component and the physiologically active component immobilized on the surface thereof, thereby forming a culture on the yarn 310 or in a space surrounded by the yarn 310 There is an advantage that cell proliferation can be further accelerated by continuing and amplifying the cell stimulation through the physiologically active component and the intracellular signal transmission through the cell stimulation.

The adhesive component is used to immobilize the cultured cells on the cell support in the initial stage so that the cells loaded on the culture solution are prevented from floating, and / or a physiologically active ingredient is fixed on the yarn yarns 310, It is possible to perform the function of preventing desorption of the supporting fibers in the course of cell culture on the yarn 310. [ The adhesive component can be used without limitation in the case of known adhesive components which are conventional biocompatibility and do not cause cytotoxicity.

Next, the physiologically active component that can be provided in the yarn 310 will be described in more detail. The physiologically active component may be a substance that directly or indirectly induces at least one of cell adhesion, migration, growth, proliferation, and differentiation so as to promote cell culture. Known substances known to be < RTI ID = 0.0 > known < / RTI > For example, the physiologically active ingredient may be a monoamine, an amino acid, a peptide, a saccharide, a lipid, a protein, a glucoprotein, a glucolipid, a proteoglycan, a mucopolysaccharide, acid, and a cell or a cell. The monoamines include, for example, compounds containing primary amines such as catecholamines and indole amines. In addition, the peptide includes an oligopeptide, and the protein includes a polypeptide, and may be, for example, fibronectin. The saccharides include monosaccharides, polysaccharides, oligosaccharides, and carbohydrates, and the lipids may be, for example, steroid hormones.

On the other hand, the physiologically active ingredient may include a motif. The motif may be a natural peptide or a recombinant peptide comprising a predetermined amino acid sequence contained in at least one selected from the group consisting of a growth factor or an extracellular matrix protein, a glycoprotein, and a proteoglycan. Specifically, the motif is selected from the group consisting of adrenomedullin, angiopoietin, bone morphogenetic protein (BMP), brain derived neurotrophic factor (BDNF), epidermal growth factor (EGF), erythropoietin, (GDNF), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), fibroblast growth factor, glial cell line derived neurotrophic factor Growth differentiation factor-9 (GDF9), hepatocyte growth factor (HGF), hepatoma-derived growth factor (HDGF), insulin-like growth factor (IGF), keratinocyte growth factor (KGF), migration-stimulating factor (MSF), myostatin (GDF-8), nerve growth factor Platelet-derived growth factor cell growth factor (TCGF), neurofilin, transforming growth factor-alpha (TGF-a), transforming growth factor- (TGF-beta), tumor necrosis factor-alpha (TNF-alpha), vascular endothelial growth factor (VEGF), IL-1, IL-2, IL-3, IL-4, IL-5, IL-7. ≪ RTI ID = 0.0 > (GF) < / RTI > Or at least one extracellular matrix selected from the group consisting of hyaluronic acid, heparin sulfate, chondroitin sulfate, termarine sulfate, keratan sulfate, alginate, fibrin, fibrinogen, collagen, elastin, fibronectin, bitonectin, carderine and laminin or a sequence of a predetermined amino acid included in an extracellular matrix. In addition, the motif may include both a predetermined amino acid sequence included in the growth factor and a known amino acid sequence contained in the extracellular matrix.

On the other hand, the motif may be integrally formed by covalently bonding to the adhesive component. For example, when the adhesive component is a protein, the motif may be directly covalently bonded to the N-terminal and / or C-terminal of the polypeptide or may be covalently bonded through a heterogeneous peptide or polypeptide. In this case, The physiologically active ingredient can be adhered firmly and the desorption of the physiologically active ingredient in the cell culture can be minimized.

When the physiologically active component is a motif, the motif may further include specific domains or motifs of known mussel proteins or mussel proteins to enhance cell adhesion.

Next, referring to FIGS. 5 and 6, the side coupling of the cell culture cartridge according to the present invention will be described.

As shown in FIGS. 5 and 6, according to an embodiment of the present invention, the first support part 100 and the second support part 200 fix and support the above-described cell culture support 300 above and below . At this time, a predetermined region (region A) at both ends of the cell culture support 300 is contacted with the first support 100 and the second support 200 to prevent shaking of the cell culture support 300, It is possible to prevent deterioration of cell uniformity due to a minute separation due to the permeation pressure of the membrane. In addition, since the cell adhesion inhibitor can be coated on the region where the A region and the frame portion 121 are in contact with the cell culture support 300, the cell can be prevented from being killed or desorbed during the culturing process or separation process The cell survival rate and the culture rate can be improved at the same time.

Next, a cell incubator 400 or 400 'including the cell culture cartridge 10 according to the present invention will be described with reference to FIGS. 7 and 8. FIG.

The cell culture solution may be a cell culture solution that promotes at least one of cell adhesion, migration, growth, proliferation, and differentiation or provides nutrients to cells according to a target cell. Do not.

As shown in FIG. 7, the cell incubator 400 according to an embodiment of the present invention may include a plurality of cell culture cartridges 10a to 10e. Although not shown in the figure, the plurality of cell culture cartridges 10a to 10e may be spaced apart from each other at fixed intervals and fixed through a fixing member in the cell incubator 400, respectively.

In addition, the cell culture cartridges 10a to 10e can be configured in the form of a cell culture cartridge array fixed and spaced apart at a predetermined interval. In this case, the cell culture cartridge array may include another fixing member so that each of the cell culture cartridges 10a to 10e can be fixed apart at a predetermined interval. Accordingly, unlike FIG. 7, the cell incubator may have a plurality of cell culture cartridges in an array form, thereby providing the same microenvironment for each cell culture and advantageous from the viewpoint of high-density cell culture. Furthermore, it is easy to separately separate the cell culture cartridge provided in one array, and the culture environment can be rapidly controlled not only before and during cell culture, but also has the advantage that the uniformity and survival rate of the cells can be improved at the same time. In addition, heterogeneous cells can be separated and cultured in a single culture environment by loading and culturing heterogeneous cells for each cartridge provided in one array.

As shown by the arrows in the figure, the cell culture liquid provides a culture material to the cells cultured on the upper and lower surfaces of the cell culture support 300 while passing through the cell culture cartridges 10a to 10e according to the present invention, (100). At this time, due to the permeability of the cell culture support 300 made of a fabric to the cell culture solution, the cell culture solution passes through the cell culture support 300 to prevent excessive pressure on the cell culture support 300, thereby preventing shaking or mechanical damage can do. Also, by uniformly circulating the upper and lower surfaces of the cell culture support 300, uniformity of cells cultured on the upper and lower surfaces of the cell culture support 300 can be improved, and cultured cells according to the area of the cell culture support 300 And the economic efficiency of the cell culture support 300 can be improved. In addition, a plurality of cell culture cartridges 10a to 10e are fixed in one cell incubator 400 to enable high-density cell culture while maintaining uniformity of cells.

As shown in FIG. 8, the cell incubator 400 'according to another embodiment of the present invention may also include a plurality of cell culture cartridges 10a to 10e, ) As the technical features are the same, redundant explanation will be omitted.

The cell culture fluid introduced through the inlet 405 of the cell incubator 400 'is transferred to the cells cultured on the upper and lower surfaces of the cell culture support 300 while passing through the cell culture cartridges 10a to 10e according to the present invention And can circulate in the cell incubator 100. [0035] Thereafter, the cell culture liquid and other substances (nutrients, waste products, products, etc.) may escape through the outlet 407.

As described above, the cell incubator (400, 400 ') including the cell culture cartridge 10 according to the present invention can easily detach the cell culture support and immobilize the cell culture support to provide a stable cell culture environment, And the shake of the cell culture support can be prevented in the process of culturing the cells, thereby enhancing the cell culture efficiency.

Also, even if the cell culture solution is dispersed in a cell culture support, it is possible to prevent shaking of the support, and uniform cell growth can be achieved by imparting the same mechanical, physical and chemical stimulation to each cell to be cultured regardless of the region of the support. Further, it is possible to cultivate high density cells suitable for the field where cells are cultured in large quantities, and cells having a shape suitable for the desired test and experiment can be cultured without damaging the cells in the process of separating the cell culture support.

Table 1 below is a table for the sequence listing showing the amino acid sequences according to the sequence numbers described in the present invention.

SEQ ID NO: Amino acid sequence One Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Gly Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Gly Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala
Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly
Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn
Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro
Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr
Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly
Gly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp
Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His
Tyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr
Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys
Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg
Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys
Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 8 Arg Gly Asp 9 Arg Gly Asp Ser 10 Arg Gly Asp Cys 11 Arg Gly Asp Val 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro 13 Gly Arg Gly Asp Ser 14 Gly Arg Gly Asp Thr Pro 15 Gly Arg Gly Asp Ser Pro 16 Gly Arg Gly Asp Ser Pro Cys 17 Tyr Arg Gly Asp Ser 18 Ser Pro Pro Arg Arg Ala Arg Val Thr 19 Trp Gln Pro Pro Arg Ala Arg Ile 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe 23 Ile Lys Val Ala Asn 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln 25 Val Ala Glu Ile Asp Gly Ile Gly Leu 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys

10: Cartridge for cell culture 100: First support part
110: rim portion 111: accommodating portion
120: center portion 121: frame portion
123: mesh portion 123a: first mesh line
123b: second mesh line 200: second support portion
210: rim portion 211: accommodating portion
220: center portion 221: frame portion
223: mesh portion 223a: first mesh line
223b: second mesh line 300: cell culture support
400, 400 ': cell incubator

<110> AMOLIFESCIENCE <120> CARTRIDGE FOR CELL CULTURE <130> 1060405 <160> 28 <170> Kopatentin 2.0 <210> 1 <211> 196 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 1 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr   1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala              20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro          35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu      50 55 60 Gly Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser  65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys                  85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly             100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr         115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr     130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys                 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro             180 185 190 Pro Thr Tyr Lys         195 <210> 2 <211> 202 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr   1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala              20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro          35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu      50 55 60 Gly Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser  65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys                  85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly             100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr         115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr     130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys                 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro             180 185 190 Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro         195 200 <210> 3 <211> 172 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr   1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala              20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro          35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala      50 55 60 Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly  65 70 75 80 Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn                  85 90 95 Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala             100 105 110 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro         115 120 125 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro     130 135 140 Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr 145 150 155 160 Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu                 165 170 <210> 4 <211> 46 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly   1 5 10 15 Gly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp              20 25 30 Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr          35 40 45 <210> 5 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His   1 5 10 15 Tyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr              20 25 30 Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys          35 40 45 Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg      50 55 60 Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser  65 70 75 <210> 6 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys   1 5 10 <210> 7 <211> 60 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro   1 5 10 15 Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys              20 25 30 Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr          35 40 45 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys      50 55 60 <210> 8 <211> 3 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 8 Arg Gly Asp   One <210> 9 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 9 Arg Gly Asp Ser   One <210> 10 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 10 Arg Gly Asp Cys   One <210> 11 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 11 Arg Gly Asp Val   One <210> 12 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro   1 5 10 <210> 13 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 13 Gly Arg Gly Asp Ser   1 5 <210> 14 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 14 Gly Arg Gly Asp Thr Pro   1 5 <210> 15 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 15 Gly Arg Gly Asp Ser Pro   1 5 <210> 16 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 16 Gly Arg Gly Asp Ser Pro Cys   1 5 <210> 17 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 17 Tyr Arg Gly Asp Ser   1 5 <210> 18 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 18 Ser Pro Pro Arg Arg Ala Arg Val Thr   1 5 <210> 19 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 19 Trp Gln Pro Pro Arg Ala Arg Ile   1 5 <210> 20 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly   1 5 10 <210> 21 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr   1 5 10 <210> 22 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe   1 5 10 <210> 23 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 23 Ile Lys Val Ala Asn   1 5 <210> 24 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln   1 5 10 15 <210> 25 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 25 Val Ala Glu Ile Asp Gly Ile Gly Leu   1 5 <210> 26 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro   1 5 10 <210> 27 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly   1 5 10 <210> 28 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys   1 5 10

Claims (12)

A cell culture support comprising a fabric;
A first support disposed on a lower surface of the cell culture support; And
And a second support portion disposed on an upper surface of the cell culture support body,
Wherein each of the first support portion and the second support portion includes a rim portion and a center portion. The method according to claim 1,
Wherein at least one protrusion is formed on a rim portion of one of the first and second support portions,
And a rim of the other support portion includes a receiving portion into which the protruding portion is inserted. The method according to claim 1,
Wherein the central portion includes a frame portion for performing a supporting function and a mesh portion through which a cell culture fluid is permeated. The method of claim 3,
Wherein a cell adhesion inhibiting substance is coated on the surface of the frame part. The method according to claim 1,
Wherein the fabric is woven from at least one of a yarn, a filament yarn and a slitting yarn. 6. The method of claim 5,
Wherein the yarn further comprises a cell culture enhancing substance. The method according to claim 6,
The cell culture enhancing substance is a physiologically active ingredient that induces at least one of cell adhesion, migration, growth, proliferation, and differentiation. 8. The method of claim 7,
The physiologically active component may be selected from the group consisting of monoamines, amino acids, peptides, saccharides, lipids, proteins, glucoproteins, glucolipids, proteoglycans, mucopolysaccharides and nucleic acids. And at least one selected from the group consisting of at least one compound selected from the group consisting of: 6. The method of claim 5,
The yarn may be selected from the group consisting of polystyrene (PS), polyethylene terephthalate (PET), polyether sulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane (PDMS) Poly (alkylene oxide), poly (amino acids), poly (allylamines), polyphosphazene, and polyethylene oxide-polypropylene oxide block copolymers At least one non-biodegradable fiber forming component selected from the group consisting of
Polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly-L-lactide), PLGA (poly-DL-lactide-co-glycolide), polylactic acid A biodegradable fiber-forming component selected from the group consisting of polylactic acid and polyvinyl alcohol. The cell culture apparatus according to any one of claims 1 to 9, wherein a plurality of cell culture cartridges are stacked. A cell culture cartridge array according to any one of claims 1 to 9, wherein a plurality of cell culture cartridges are fixed with a predetermined spacing therebetween. 11. A cell incubator comprising the cell culture cartridge array according to claim 10.

Images