KR102386537B1 - SHEATH-CORE TYPE BICOMPONENT FIBER for cell culture scaffold AND FABRIC COMPRSING THE SAME - Google Patents

Abstract

A sheath-core type composite fiber for a cell culture support is provided. The composite fiber according to an exemplary embodiment of the present invention includes a core part and a sheath part surrounding the outer surface of the core part and containing a cell culture improving component. According to this, a microenvironment suitable for attachment, migration, proliferation, and differentiation of cells to be cultured is realized, so that the survival rate of cells is improved and the cells can be three-dimensionally proliferated. In addition, since the support according to the present invention exhibits sufficient mechanical strength to prevent collapse of the support, etc. occurring during the cell culture process, cells can be stably proliferated. In addition, in the process of recovering the cells, they can be easily separated from the support without damaging the cells.

Description

Sheath-core type composite fiber for cell culture scaffold and fabric for cell culture scaffold including the same

The present invention relates to a sheath-core type composite fiber, and more particularly, to a sheath-core type composite fiber for a cell culture support.

Recently, as the use of cultured cells for disease treatment is expanded, interest and research on cell culture are increasing. Cell culture is a technology for collecting cells from a living body and culturing them outside the living body. The cultured cells are differentiated into various tissues of the body such as skin, organs, and nerves to be transplanted into the human body or transplanted into the human body before differentiation to engraft and differentiate. It can be used to treat various diseases by making it happen at the same time.

The field related to cell culture is tissue engineering, and it is a multidisciplinary study that applies existing scientific fields such as cytology, life science, engineering, and medicine. The correlation between the structure and function of living tissue A new fusion technology is being researched to understand and regenerate damaged tissues or organs with normal tissues.

One of the tasks that continues to receive a lot of attention and is researched/developed in the field of conventional cell culture or tissue engineering using the same is the study of materials, structures, etc. of a support capable of culturing/differentiating cells. In other words, in order to examine the effect of a specific substance on the human body, the toxicity reaction experiment of a specific substance using cultured cells is more similar to the real thing than when cultured/differentiated cell aggregates with a three-dimensional structure similar to the actual human cell structure. It may be suitable as an in vitro cytotoxicity test model. In addition, in order to transplant cultured cells into human tissue, when a cultured/differentiated cell aggregate or tissue is transplanted into a three-dimensional structure similar to the actual tissue of the human body, the transplanted cells or tissues can sufficiently perform their functions and roles. .

However, the cells developed so far for cell culture or tissue engineering are not cultured in a structure similar to the body, and the cell viability is not high. there is a problem. That is, when the scaffold for cell culture or tissue engineering is prepared with nanofibers suitable for the size of cells, there may be problems in cell culture due to weak mechanical properties.

In addition, the yarn for cell culture or tissue engineering support may contain a cell culture-enhancing material necessary for cell culture. There is a problem that the passability is significantly lowered.

Furthermore, as the yarn is cut by an external force during cell culture, there may be a problem in that the cells are easily detached.

On the other hand, in order to move the cultured cells to the target, a process of recovering the cells from the support is required. In the case of a conventional cell culture yarn, it is very difficult to separate the cultured cells due to poor cell recovery.

Accordingly, a culture environment similar to that of the body can be provided, the space required for cell culture is properly secured for each cell, and at the same time, sufficient mechanical strength is expressed, it is easy to manufacture in a desired structure, and while the cells are cultured in the support. There is an urgent need to develop a yarn for a cell culture support capable of preventing cells from escaping to improve cell viability, grow cells three-dimensionally, and improve recovery rate for cultured cells.

Registered Patent Publication No. 1104305

The present invention was devised in view of the above points, and it is an object of the present invention to provide a sheath-core type composite fiber for a cell culture support that can be easily detached without damaging the cells when the cultured cells are separated from the support. .

In addition, a microenvironment suitable for cell adhesion, migration, proliferation, and differentiation is implemented, thereby improving cell viability, and providing a cis-core type composite fiber for a cell culture support capable of three-dimensionally proliferating cells.

In addition, the present invention provides a sheath-core type composite fiber for a cell culture support in which cells can be stably proliferated as it has sufficient mechanical strength to prevent the collapse of the support occurring during the cell culture process, and It has a different purpose.

In addition, the present invention provides a cis-core type composite fiber for a cell culture support capable of culturing the shape/structure of cultured cells to be more suitable for application for transplantation in an in vitro experimental model or in an animal body similar to that of an actual animal body. Another purpose is to provide

In addition, the present invention provides a fabric for a cell culture support through the sheath-core type composite fiber for a cell culture support according to the present invention, a bioreactor including the same, a cell culture container, etc. Various products used in the field of cell culture or tissue engineering. There is another purpose to implement.

In order to solve the above problems, the present invention provides a sheath-core type composite fiber for a cell culture support having a sheath portion surrounding the outside of the core portion, including a core portion and a cell culture enhancing material.

In addition, the core portion may have an elastic recovery rate of 50% or more.

In addition, the core part may perform a supporting function for the sheath part.

In addition, the composite fiber may have an elongation of 10 to 500%.

In addition, the composite fiber may have a strength of 1 g/de to 50 g/de.

In addition, the cell culture enhancing material may be a physiologically active ingredient that induces any one or more of adhesion, migration, growth, proliferation, and differentiation of cells.

In addition, at least a portion of the physiologically active ingredient may be disposed inside the sheath, and the remaining portion may be provided to protrude to the outside or coated on the outer surface of the sheath.

In addition, the physiologically active ingredient is monoamine, amino acid, peptide, saccharide (saccharide), lipid (lipid), protein, glycoprotein (glucoprotein), glycolipid (glucolipid), proteoglycan, mucopolysaccharide (mucopolysaccharide) and nucleic acid (nucleic acid) ) may include any one or more of any one or more compounds and cells selected from the group consisting of.

In addition, the sheath part contains polystyrene (PS), polyethylene terephthalate (PET), polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane ( PDMS), polyamide, polyalkylene, polyalkylene oxide, poly(amino acids), poly(allylamines), polyphosphazene and polyethylene oxide -Any one or more non-biodegradable components selected from the group consisting of polypropylene oxide block copolymers or polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly(L-lactide) )), PLGA (poly(DL-lactide-co-glycolide)), polylactic acid, and polyvinyl alcohol may contain any one or more biodegradable components selected from the group consisting of.

In addition, the present invention provides a fabric for a cell culture support comprising the sheath-core type composite fiber for a cell culture support according to the present invention.

In addition, it provides a fabric for a cell culture support and an implant for tissue engineering including cells cultured on the fabric.

In addition, the cells are selected from the group consisting of hematopoietic stem cells, hepatocytes, fibroblasts, epithelial cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipocytes, chondrocytes, osteocytes, blood cells and skin cells. It may include any one or more cells.

The cis-core type composite fiber for a cell culture support according to the present invention can easily implement a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells, thereby improving the cell viability and proliferation rate and three-dimensionally proliferating the cells. It can form a support in the presence or perform the function of a support by itself. In addition, it is very easy to separate cultured cells in a cluster state without damage due to excellent mechanical strength and excellent elasticity. Furthermore, even if functional materials for improving cell culture are contained in the composite fiber, mechanical strength for stably culturing cells is guaranteed. It can be widely applied.

1 is a perspective view showing a sheath-core type composite fiber for a cell culture support according to an embodiment of the present invention;
2 is a view showing a sheath-core type composite fiber for a cell culture support containing a physiologically active ingredient according to an embodiment of the present invention, and
3a to 3c relate to a fabric including a sheath-core type composite fiber for a cell culture support according to an embodiment of the present invention, and FIG. 3a is a view showing a fabric for a cell culture support, FIG. 3b is a knitted fabric A view showing a fabric for a phosphorus cell culture support, FIG. 3c is a view showing a fabric for a cell culture support, which is a nonwoven fabric.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and the same reference numerals are added to the same or similar elements throughout the specification.

When cells are cultured on a support composed of nanofibers in consideration of the size of the cells, the mechanical properties of the nanofibers are weak, making it difficult for the cells to be stably attached. In addition, due to the flow of nanofibers, agglomerates of cells attached to the surface of the nanofibers are formed non-uniformly, so there is a difficulty in obtaining uniform cultured cells.

In addition, when a cell culture enhancing material is further provided in the support for efficient cell culture, compatibility with the components forming the support is not good, so the mechanical strength of the support is significantly lowered, so a stable environment necessary for cell culture can be provided. There is no problem.

Furthermore, in order to move the cultured cells to the target, a process of recovering the cells from the support is required. Since the conventional cell culture support has poor cell recovery, it is very difficult to separate the cultured cells on the support, and in the process of separating the cultured cells There was a problem that cells were easily damaged.

In order to solve this, the present invention is for a cell culture support having a sheath portion 200 surrounding the outside of the core portion 100, including a core portion 100 and a cell culture enhancing material, as shown in FIG. The sheath-core type composite fiber 10 is implemented.

First, the core part 100 performs a support function for the cell culture support, and through this, the sheath-core type composite fiber 10 is easy to achieve a certain level of mechanical properties such as elongation, strength, and the like.

To this end, the composite fiber 10 may have a strength of 1 g/de (denier) to 50 g/de. If the composite fiber 10 has a strength of less than 1 g/de, the support may be bent or cut in the process of manufacturing, storage and cell culture of the cell support, and if the strength exceeds 50 g/de, excessive strength Due to this, it may be difficult to achieve the desired effect, such as a decrease in cell recovery.

In addition, the composite fiber 10 may have an elongation of 10% to 500%. If the elongation of the composite fiber 10 is less than 10%, it may be difficult to achieve the desired effect such as a decrease in cell recovery due to insufficient elasticity, and if it exceeds 500%, it is difficult to stably culture the cells, and culture The cell support can be easily deformed, vibrated and broken by external shaking in the process and the flow of the cell culture medium, so it may be difficult to serve as a support.

In addition, the fineness of the composite fiber 10 may be determined in consideration of the type and size of cells to be cultured, and the number of years and fineness of the sheath 200, preferably 10 nm to 10 μm. If the fineness is less than 10 nm, it may be difficult to prepare a cell aggregate to a desired level due to a decrease in the specific surface area to which cells are attached, and the pore size exceeding 10 μm in fineness may be reduced, thereby reducing the cell proliferation rate.

The core part 100 may be implemented with a fiber-forming component known in the textile field. for example, The fiber-forming component is polystyrene (PS), polyethylene terephthalate (PET), polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), depending on the type of cell cultured and the culture purpose. ), polydimethylsiloxane (PDMS), polyamide, polyalkylene, polyalkylene oxide (poly(alkylene oxide)), polyamino acids (poly(amino acids)), polyallylamine (poly(allylamines), polyphosphazene (polyphosphazene) and any one or more non-biodegradable components selected from the group consisting of polyethylene oxide-polypropylene oxide block copolymers, or polycaprolactone, polydioxanone, polyglycolic acid, Any one or more biodegradable ingredients selected from the group consisting of poly(L-lactide) (PLLA), poly(DL-lactide-co-glycolide) (PLGA), polylactic acid and polyvinyl alcohol may include

Next, the mono-yarn of the core part 100 may be a spun yarn, a filament yarn, or a slitting yarn.

When the monofilament is a spun yarn or a filament yarn, it may be changed to suit the type and size of the cells to be cultured, and the shape and size of the cell aggregate.

In addition, the spun yarn may be prepared through raw cotton by a known method. In addition, the filament yarn may be manufactured by spinning by a known method, and the spinning may be a known spinning method such as chemical spinning or electrospinning.

In addition, the slitting yarn may be manufactured by cutting a sheet-like fiber aggregate, fabric, etc. to have a predetermined width. Preferably, the slitting yarn may be a mono yarn manufactured by cutting a sheet-like fibrous web having a three-dimensional network structure to have a predetermined width. In this case, the fibrous web may be compressed with a constant pressure to improve the ease of the slitting process and increase the strength of the slitting yarn. For example, the slitting yarn may be a mono yarn obtained by cutting a fibrous web having a basis weight of 0.1 to 100 g/m 2 to a width of 0.1 to 30 mm. If the slitting width is less than 0.1 mm, cutting is not easy, and there is a problem in that it can be easily trimmed by tension and rotational force applied during false twisting. In addition, when slitting with a width exceeding 30 mm, there is a problem that non-uniform twisting may occur during continuous yarn.

As described above, the sheath-core type composite fiber 10 for a cell culture support according to the present invention is composed of a sheath portion 200 to which cells for culture are attached and a core portion 100 having a supporting function for the sheath portion 100 during cell culture. Due to the improved mechanical properties of the composite fiber 10, it is possible to solve the problem of cells detaching from the support.

In addition, the mechanical properties of the composite fiber 10 are improved, so that the cutting rate or breakage rate of the composite fiber 10 and the fabric can be greatly reduced, and the cells are easily detached from the support due to breakage of the support during cell culture, or cell proliferation rate due to this It can prevent degradation and provide a stable environment for cell culture.

Next, according to another embodiment of the present invention, the core part 100 may include a fiber-forming component having an elastic recovery rate of 50% or more. In this case, the elasticity of the complex fiber 10 is improved, so that the cells cultured in the sheath 200 can be easily recovered. If the fiber-forming component of the core part 100 has an elastic recovery rate of less than 50%, the cells may be damaged in the process of separating the cells cultured in the sheath part 200, and thus the cell recovery rate may be reduced.

Accordingly, the fiber-forming component of the core part 100 may include a known urethane-based or a known elastomer having elasticity. For example, one or more materials selected from polyurethane, polyurethaneurea, polyester-based, polyolefin-based, polyamide-based, polystyrene-based, and the like may be included as a fiber-forming component.

As described above, according to an embodiment of the present invention, the composite fiber 10 having the core part 100 including the fiber-forming component having an elastic recovery rate of 50% or more is obtained by separating the cells cultured in the sheath part 200 to obtain a target body. Cells can be easily recovered without damaging the cells in the process of moving them.

Next, a description will be given of the sheath portion 200 provided to surround the above-described core portion 100 and providing a space in which cells are cultured.

The sheath 200 may be implemented with a known fiber-forming component 210, may be implemented by selecting a suitable material according to the type of support, and may select a material differently depending on a special purpose such as degradability is required. Accordingly, the present invention is not particularly limited thereto. The fiber-forming component 210 may include a cellulose component such as cotton, hemp, or the like, a protein component such as wool or silk, or a natural fiber component such as a mineral component. Alternatively, the fiber-forming component 210 may be a known artificial fiber component.

On the other hand, the fiber forming component 210 is polystyrene (PS), polyethylene terephthalate (PET), polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), Polydimethylsiloxane (PDMS), polyamide, polyalkylene, poly(alkylene oxide), poly(amino acids), polyallylamine (poly(allylamines), polyphosphazene ) and polyethylene oxide-polypropylene oxide block copolymer of any one or more non-biodegradable components selected from the group consisting of, or polycaprolactone, polydioxanone, polyglycolic acid, PLLA ( Contains any one or more biodegradable ingredients selected from the group consisting of poly(L-lactide)), PLGA (poly(DL-lactide-co-glycolide)), polylactic acid and polyvinyl alcohol can do.

On the other hand, the aforementioned sheath 200 may include one or more cell culture enhancing materials such as the adhesive component 220 and the physiologically active component 230 in addition to the fiber forming component 210 . At this time, the adhesive component 220 or the physiologically active component 230 is not provided inside the sheath 200, and the physiologically active component 230 is applied to the sheath through the adhesive component 220 fixed to one area of the outer surface. It may be fixed to a portion of the outer surface of the unit 200 . Alternatively, the physiologically active component 230 may be provided to completely cover the adhesive component 220 provided by covering the entire outer surface of the sheath 200 .

Cell culture technology that is being studied recently is being studied in the direction of realizing the actual intercellular environment in the body in vitro . There is a trend to apply various components to the culture solution during in vitro culture. However, if the culture solution contains a substance that can promote cell culture, there is a limit to continuously exposing the substance to the cultured cells. There is a problem in increase and proliferation efficiency.

Accordingly, the adhesive component 220 and the physiologically active component 230 are provided on the support in a fixed state to the sheath 200 according to an embodiment of the present invention to sustain and amplify cell stimulation and intracellular signal transduction through it. This has the advantage of further accelerating cell proliferation.

The adhesive component 220 is a function of fixing the cultured cells on the cell support at the beginning to prevent the cells loaded on the culture solution from floating and/or fixing the physiologically active component 230 to the sheath 200 The physiologically active component 230 may perform a function of preventing detachment from the support fibers in the process of cell culture on the sheath unit 200 . The adhesive component 220 may be used without limitation in the case of a known adhesive component 220 that does not generate cytotoxicity due to its normal biocompatibility, but preferably the amino acid sequence of SEQ ID NOs: 1 to 7 is used once. It may include one or more selected from the group consisting of a protein made by repeating to 20 times and a protein in which at least two of these proteins are fused, through which cytotoxicity is significantly reduced, and the adhesion of the physiologically active ingredient 230 is excellent. At the same time, there is an advantage that the detachment of the physiologically active component or isolation of the cells generated as the adhesive component 220 is dissolved in the culture solution during cell culture can be prevented.

Next, the physiologically active ingredient 230 will be described in more detail. The physiologically active ingredient 230 may be a substance that directly/indirectly induces any one or more of cell adhesion, migration, growth, proliferation, and differentiation so as to promote cell culture, such as In the case of a known substance known to express a function, it may be included without limitation. For example, the physiologically active ingredient 230 is monoamine, amino acid, peptide, saccharide, lipid, protein, glycoprotein, glycolipid (glucolipid), proteoglycan, mucopolysaccharide and It may include any one or more of any one or more compounds and cells selected from the group consisting of nucleic acids. In this case, the monoamine includes, for example, a compound containing a primary amine such as catecholamine and indoleamine. In addition, the peptide includes an oligopeptide, and the protein includes a polypeptide, and may be, for example, fibronectin. The saccharides include monosaccharides, polysaccharides, oligosaccharides and carbohydrates, and the lipid may be, for example, a steroid hormone.

Meanwhile, the physiologically active ingredient 230 may include a motif. The motif may be a natural or recombinant peptide comprising a predetermined amino acid sequence provided in any one or more selected from proteins, glycoproteins, and proteoglycans included in growth factors or extracellular matrix. Specifically, the motif is adrenomedullin (Adrenomedullin), angiopoietin (Angiopoietin), bone morphogenetic protein (BMP), brain-derived neurotrophic factor (BDNF), epidermal growth factor (EGF), erythropoietin (Erythropoietin), fibroblasts Fibroblast growth factor, glial cell line-derived neurotrophic factor (GDNF), Granulocyte colony-stimulating factor (G-CSF), Granulocyte macrophage colony-stimulating factor (GM-) CSF), growth differentiation factor-9 (GDF9), hepatocyte growth factor (HGF), hepatoma-derived growth factor (HDGF), insulin-like growth factor , IGF), keratinocyte growth factor (KGF), migration-stimulating factor (MSF), myostatin (Myostatin, GDF-8), nerve growth factor (Nerve growth factor, NGF), Platelet-derived growth factor (PDGF), thrombopoietin (TPO), T-cell growth factor (TCGF), neuropilin, transforming growth factor-alpha (TGF) -α), transforming growth factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), IL-1, IL-2, IL-3, IL-4 , IL-5, IL-6 and IL-7 may include a predetermined amino acid sequence included in any one or more growth factors (GF) selected from the group consisting of. Or, at least one extracellular matrix selected from the group consisting of hyaluronic acid, heparin sulfate, chondroitin sulfate, termatin sulfate, keratan sulfate, alginate, fibrin, fibrinogen, collagen, elastin, fibronectin, vitronectin, cadherin and laminin It may include a sequence of a predetermined amino acid included in (extracellular matrix). In addition, the motif may include both a predetermined amino acid sequence included in the growth factor and a predetermined amino acid sequence included in the extracellular matrix. More preferably, the motif may include one or more selected from the group consisting of a protein comprising the amino acid sequence of SEQ ID NO: 8 to SEQ ID NO: 28 and a protein in which at least two of these proteins are fused, but is limited thereto not.

Meanwhile, the motif may be integrally implemented by covalently bonding to the above-described adhesive component 220 . For example, when the adhesive component 220 is a protein, the motif may be directly covalently bound to the N-terminus and/or C-terminus of the polypeptide or may be covalently bound by interposing a heterogeneous peptide or polypeptide, in this case The physiologically active ingredient 230 can be more firmly attached to the sheath 200 , and detachment of the physiologically active ingredient 230 during cell culture can be minimized.

In addition, when the physiologically active ingredient 230 is a motif, the motif may be implemented to further include a known mussel protein or a specific domain or motif among mussel proteins in order to promote cell adhesion.

Referring to FIG. 2 , the sheath 200 according to the present invention may include, as described above, an adhesive component 220 and a physiologically active component 230 as a cell culture enhancing material in addition to the fiber forming component 210 .

Specifically, the adhesive component 220 may be fixed to the surface of the sheath 200 made of the biodegradable or non-biodegradable fiber-forming component 210 with an adhesive component 220, and in this case, the adhesive component 220 is provided so that a part is located inside the sheath 200 and the other part is located outside, so that it can be fixed to the surface of the sheath 200 . In addition, it is not provided inside the sheath 200, the adhesive component 220 may be fixed to one area of the outer surface. In addition, it may be provided by covering the entire outer surface of the sheath portion (200).

3A to 3C, the composite fiber 10 for a cell culture support according to the present invention may implement any one of a woven fabric, a knitted fabric, and a non-woven fabric.

As shown in Fig. 3a, in the case of the fabric 10', which is a fabric composed of the composite fiber 10 for the cell culture support according to the present invention, the fibers included in the fabric have a longitudinal and transverse direction, and the specific tissue is a plain weave, It may be a twill weave, etc., and the density of the warp and weft yarns is not particularly limited.

Next, as shown in FIG. 3b , in the case of a knitted fabric 10'' composed of a composite fiber 10 for a cell culture support according to the present invention, the knitted fabric may be made of a known knitted tissue. For example, it may be a weft knitted fabric, a warp knitted fabric, etc., but is not particularly limited thereto.

Next, as shown in Figure 3c, in the case of the nonwoven fabric (10'''), which is a nonwoven fabric composed of the composite fiber 10 for a cell culture support according to the present invention, the nonwoven fabric means that the fibers included have no longitudinal and transverse directionality, and , chemical bonding nonwovens, thermal bonding nonwovens, dry nonwovens such as airlay nonwovens, wet nonwovens, spanless nonwovens, needle punching nonwovens or melt blown nonwovens can be used. The present invention is not particularly limited thereto because it may vary depending on the mechanical strength. For example, the nonwoven fabric may be a nanofiber web formed of electrospun nanofibers.

On the other hand, the present invention can implement an implant for tissue engineering including the cultured cells by transplanting the cultured cells to the above-described fabric (10', 10'', 10''') according to the present invention.

In addition, the cells are totipotent stem cells, pluripotent stem cells, pluripotent stem cells, oligopotent (oligopotent) stem cells and any one or more stem cells selected from the group consisting of single stem cells, and hematopoietic stem cells, hepatocytes, fibroblasts, epithelium It may include one or more types of differentiated cells selected from the group consisting of cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipocytes, chondrocytes, osteocytes, blood cells, and skin cells. For example, the cell may be a cell having an elongated shape in one direction rather than a spherical shape or a cell with strong mobility.

In addition, if the material of the fabric (10', 10'', 10''') is implemented as a fiber-forming component that is harmless to the human body, the support to which the cultured cells are attached can be directly transplanted into the human body. There is an advantage in that cells can be more easily and stably engrafted in a tissue.

The present invention described above is not limited to the above-described embodiments and the accompanying drawings, and it is common in the technical field to which the present invention pertains that various substitutions, modifications and changes are possible within the scope without departing from the technical matters of the present invention. It will be clear to those who have the knowledge of Therefore, the scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention.

Table 1 below is a table for a sequence listing showing the amino acid sequence according to SEQ ID NO: described in the present invention.

SEQ ID NO: amino acid sequence One Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr
Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala
Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly
Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn
Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro
Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr
Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly
Gly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp
Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His
Tyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr
Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys
Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg
Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys
Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 8 Arg Gly Asp 9 Arg Gly Asp Ser 10 Arg Gly Asp Cys 11 Arg Gly Asp Val 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro 13 Gly Arg Gly Asp Ser 14 Gly Arg Gly Asp Thr Pro 15 Gly Arg Gly Asp Ser Pro 16 Gly Arg Gly Asp Ser Pro Cys 17 Tyr Arg Gly Asp Ser 18 Ser Pro Pro Arg Arg Ala Arg Val Thr 19 Trp Gln Pro Pro Arg Ala Arg Ile 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe 23 Ile Lys Val Ala Asn 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln 25 Val Ala Glu Ile Asp Gly Ile Gly Leu 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys

10: Sheath-core type composite fiber for cell culture support
100: core part 200: sheath part
210: cell-forming component 220: adhesive component
230: physiologically active ingredient 10', 10'', 10''': fabric

<110> AMOLIFESCIENCE <120> SHEATH-CORE TYPE BICOMPONENT FIBER for cell culture scaffold AND FABRIC COMPRSING THE SAME <130> 1060400 <160> 28 <170> KopatentIn 2.0 <210> 1 <211> 196 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 1 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu 50 55 60 Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser 65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys 85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly 100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr 115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr 130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 180 185 190 Pro Thr Tyr Lys 195 <210> 2 <211> 202 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu 50 55 60 Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser 65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys 85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly 100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr 115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr 130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 180 185 190 Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro 195 200 <210> 3 <211> 172 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala 50 55 60 Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly 65 70 75 80 Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn 85 90 95 Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala 100 105 110 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 115 120 125 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro 130 135 140 Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr 145 150 155 160 Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu 165 170 <210> 4 <211> 46 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly 1 5 10 15 Gly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp 20 25 30 Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr 35 40 45 <210> 5 <211> 76 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His 1 5 10 15 Tyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr 20 25 30 Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys 35 40 45 Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg 50 55 60 Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser 65 70 75 <210> 6 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 1 5 10 <210> 7 <211> 60 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 1 5 10 15 Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys 20 25 30 Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr 35 40 45 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 50 55 60 <210> 8 <211> 3 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 8 Arg Gly Asp One <210> 9 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 9 Arg Gly Asp Ser One <210> 10 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 10 Arg Gly Asp Cys One <210> 11 <211> 4 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 11 Arg Gly Asp Val One <210> 12 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro 1 5 10 <210> 13 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 13 Gly Arg Gly Asp Ser 1 5 <210> 14 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 14 Gly Arg Gly Asp Thr Pro 1 5 <210> 15 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 15 Gly Arg Gly Asp Ser Pro 1 5 <210> 16 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 16 Gly Arg Gly Asp Ser Pro Cys 1 5 <210> 17 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 17 Tyr Arg Gly Asp Ser 1 5 <210> 18 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 18 Ser Pro Pro Arg Arg Ala Arg Val Thr 1 5 <210> 19 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 19 Trp Gln Pro Pro Arg Ala Arg Ile 1 5 <210> 20 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 1 5 10 <210> 21 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr 1 5 10 <210> 22 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe 1 5 10 <210> 23 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 23 Ile Lys Val Ala Asn 1 5 <210> 24 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln 1 5 10 15 <210> 25 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 25 Val Ala Glu Ile Asp Gly Ile Gly Leu 1 5 <210> 26 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro 1 5 10 <210> 27 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 1 5 10 <210> 28 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Motif for cell culture scaffold <400> 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys 1 5 10

Claims (12)

a core part having an elastic recovery rate of 50% or more; and
It wraps around the outside of the core part and has a sheath part containing a cell culture enhancing material on which a physiologically active ingredient is fixed on the surface through an adhesive component, the strength is 1 g/de to 50 g/de, and the elongation is 10% to 500% A sheath-core type composite fiber for a phosphorus cell culture support. delete delete The method of claim 1,
The cell culture enhancing material is a cis-core type composite fiber for a cell culture support, which is a physiologically active ingredient that induces at least one of cell adhesion, migration, growth, proliferation, and differentiation. delete 5. The method of claim 4,
The physiologically active ingredients are monoamines, amino acids, peptides, saccharides, lipids, proteins, glycoproteins, glycolipids, proteoglycans, mucopolysaccharides and nucleic acids. A cis-core type composite fiber for a cell culture support comprising any one or more of any one or more compounds and cells selected from the group consisting of. A cell culture support fabric comprising a sheath-core type composite fiber for a cell culture support according to any one of claims 1, 4 and 6. The cell culture support fabric according to claim 7; and
A tissue engineering implant comprising cells cultured on the fabric. 9. The method of claim 8,
The cells are any one selected from the group consisting of hematopoietic stem cells, hepatocytes, fibroblasts, epithelial cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipocytes, chondrocytes, osteocytes, blood cells and skin cells. An implant for tissue engineering comprising the above cells. delete delete delete

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