KR102527073B1 - Blended yarn for cell culture scaffold and fabric comprising the same - Google Patents

Abstract

A mixed filament yarn for a cell culture support is provided. A mixed filament yarn for a cell culture support according to an embodiment of the present invention is coated with a first yarn having a support function and at least a portion of an outer surface of the first yarn, and a second yarn comprising nanofibers having an average diameter of 1000 nm or less; is implemented including According to this, a microenvironment suitable for attachment, migration, proliferation, and differentiation of cultured cells is implemented, thereby improving cell viability and allowing cells to proliferate in three dimensions. During the cell culture process, cells can be stably proliferated as sufficient mechanical strength is expressed to prevent collapse of the scaffold that occurs.

Description

Blended yarn for cell culture scaffold and fabric for cell culture scaffold containing the same {Blended yarn for cell culture scaffold and fabric comprising the same}

The present invention relates to a mixed filament yarn, and more specifically, a microenvironment suitable for the attachment, migration, proliferation, and differentiation of cultured cells is implemented to improve the survival rate of cells, and even under culture conditions in which cell culture medium flows or under vibration conditions. It relates to a mixed filament yarn for a cell culture support that secures mechanical properties capable of three-dimensionally stable growth of cells so as to be cultured as a three-dimensional cell cluster, and a fabric for a cell culture support including the same.

Recently, as the use of cultured cells for disease treatment is expanding, interest in and research on cell culture are increasing. Cell culture is a technique of collecting cells from a living body and culturing them outside the living body. The cultured cells are differentiated into various tissues of the body such as skin, organs, and nerves, and transplanted into the human body or transplanted into the human body in a state before differentiation to engraft and differentiate. It can be used for the treatment of various diseases by making it happen at the same time.

Tissue engineering is a field in which cell culture is linked, and it is a multidisciplinary study that applies existing scientific fields such as cytology, life science, engineering, and medicine. New convergence technologies are being researched to understand and replace damaged tissues or organs with normal tissues and regenerate them.

In the field of conventional cell culture or tissue engineering using the same, one of the subjects that are continuously receiving a lot of attention and being researched/developed is research on materials and structures of scaffolds capable of culturing/differentiating cells. In other words, the toxicity reaction experiment of a specific substance using cultured cells to examine the effect of a specific substance on the human body is more realistic than when it is conducted through a cultured/differentiated cell cluster with a three-dimensional structure similar to the actual human cell structure. It can be suitable as an in vitro cytotoxicity test model. In addition, in order to transplant cultured cells into tissues of the human body, when cultured/differentiated cell aggregates or tissues are transplanted into a three-dimensional structure similar to actual tissues of the human body, the transplanted cells or tissues can fully function and function. .

However, scaffolds for cell culture or tissue engineering that have been developed so far do not culture cells with a structure similar to that of the body and do not have a high cell survival rate. there is a problem. That is, when a support for cell culture or tissue engineering is made of nanofibers suitable for the size of cells, mechanical properties may be weak and there may be problems in cell culture.

In addition, the yarn for cell culture or tissue engineering support may contain functional materials necessary for cell culture. In this case, the mechanical strength of the yarn is weakened due to the functional material contained in the yarn, so that the yarn is cut during cell culture or by external force. There may be a problem that the cells are easily released.

Accordingly, a culture environment similar to that of the body can be provided, the space required for cell culture is appropriately secured for each cell, while sufficient mechanical strength is expressed, it is easy to manufacture into a desired structure, and the cells are cultured in the support. There is an urgent need to develop a scaffold capable of preventing cells from escaping and allowing cell viability and three-dimensional growth of cells.

Registered Patent Publication No. 1104305

The present invention was devised in view of the above points, It is an object of the present invention to provide a mixed filament yarn for a cell culture support with improved cell viability by implementing a microenvironment suitable for cell attachment, migration, proliferation, and differentiation, and a fabric for a cell culture support including the same.

In addition, the present invention is a mixed filament yarn for cell culture supports in which cells are stable by expressing sufficient mechanical strength to prevent collapse of the supports that occur during the cell culture process, and can be multidimensionally proliferated like a three-dimensional cell cluster. And there is another object to provide a fabric for a cell culture support comprising the same.

Furthermore, the present invention is a yarn for cell culture support capable of culturing the shape / structure of cultured cells similar to the actual animal body so that the cultured cells are more suitable for application to in vitro experimental models or transplantation into the animal body, and Another object is to provide a fabric for a cell culture support comprising the same.

In order to solve the above problems, the present invention is a cell comprising a first yarn having a support function, and a second yarn coated on at least a portion of the outer surface of the first yarn and including nanofibers having an average diameter of 1000 nm or less. A blended yarn for a culture support is provided.

According to one embodiment of the present invention, the first yarn may be a single yarn or a multi yarn having a plurality of mono yarns, and may have an average diameter of 100 to 1000 μm.

In addition, when the first yarn is multi-yarn, the number of strands may be 2 to 10.

In addition, the mixed filament yarn may be formed by covering the outside of the first yarn with a second yarn having a slitting yarn obtained by cutting a nanofiber web having a three-dimensional network structure formed of nanofibers to a predetermined width. In this case, the slitting yarn may have a basis weight of 5 to 30 g/m 2 , a thickness of 7 to 30 μm, and a ratio of basis weight to thickness of 1: 0.8 to 1.7.

In addition, the first yarn may be a multi yarn having a plurality of mono yarns so that the covered slitting yarn has a three-dimensional surface morphology.

In addition, the first yarn and the second yarn may be provided in a weight ratio of 1: 0.5 to 10.

In addition, the second yarn may be a nanofiber assembly formed of nanofibers electrospun on the outer surface of the first yarn.

In addition, the second yarn may be a nanofiber electrospun on the outer surface of the first yarn.

In addition, the nanofiber assembly may include a surface portion in which the nanofibers cover the outer surface of the first yarn, and a protrusion portion extending from the surface portion and protruding a portion of the nanofibers toward the outside.

In addition, in order to improve the adhesion between the nanofiber assembly and the first yarn, the first yarn may be a multi yarn having a plurality of mono yarns.

In addition, the thickness of the surface portion may be 0.2 ~ 5㎛.

In addition, in order to improve adhesion between the surface portion and the first yarn, some of the nanofibers of the surface portion may be partially or entirely bonded to each other to cover the outside of the first yarn.

In addition, the weight ratio of the first yarn and the nanofiber assembly may be 1: 2 to 5.

In addition, the first yarn or the second yarn is each independently a fiber forming component polystyrene (PS), polyester, polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN) , polydimethylsiloxane (PDMS), polyamide, polyalkylene, poly(alkylene oxide), polyurethane, poly(amino acids), poly(allylamines), poly At least one non-biodegradable component selected from the group consisting of phosphazene and polyethylene oxide-polypropylene oxide block copolymer, or polycaprolactone, polydioxanone, and polyglycolic acid , PLLA (poly (L-lactide)), PLGA (poly (DL-lactide-co-glycolide)), polylactic acid (Polylactic acid) and polyvinyl alcohol (polyvinyl alcohol) biodegradable at least one selected from the group consisting of components may be included.

In addition, the second yarn may include a physiologically active component that induces any one or more of cell attachment, migration, growth, proliferation, and differentiation.

In addition, the physiologically active component is monoamine, amino acid, peptide, saccharide, lipid, protein, glycoprotein, glycolipid, proteoglycan, mucopolysaccharide and nucleic acid ) may include any one or more of any one or more compounds and cells selected from the group consisting of.

In addition, the present invention provides a fabric for a cell culture support comprising the mixed filament yarn for a cell culture support according to the present invention.

In addition, the present invention provides a fabric for a cell culture support according to the present invention and an implant for tissue engineering comprising cells cultured on the fabric.

According to one embodiment of the present invention, the cells are hematopoietic stem cells, hepatocytes, fibrocytes, epithelial cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipocytes, chondrocytes, bone cells, blood cells and It may be an implant for tissue engineering that is any one or more cells selected from the group consisting of skin cells.

Hereinafter, terms used in the present invention will be described.

The "extracellular matrix (ECM)" of the present invention is a matrix surrounding the outside of cells, occupied between cells, and means having a network structure mainly composed of proteins and polysaccharides.

The "motif" of the present invention is included in extracellular matrix proteins, glycoproteins, etc., which play an important role in cell adhesion, migration, and differentiation, and can structurally / functionally interact with receptors provided to penetrate the surface or membrane of the cell membrane. As a peptide containing an amino acid sequence that is present, it includes all those isolated from cells or artificially produced using gene cloning techniques.

The "3-dimensional cell cluster" of the present invention means a shape in which cells are three-dimensionally gathered in three dimensions.

The mixed filament yarn for cell culture support according to the present invention easily implements a microenvironment suitable for the attachment, migration, proliferation, and differentiation of cultured cells to improve the survival rate and proliferation rate of cells and form a support capable of three-dimensionally proliferating cells, or It can perform the function of the support itself. In addition, as the mechanical properties that allow cells to grow in a three-dimensionally stable manner so that they can be cultured as a three-dimensional cell aggregate even under conditions where cell culture medium flows or vibrations are present are ensured, they are suitable for use in cell culture fields such as bioreactors and cell culture containers or tissue engineering fields. It can be widely applied to various products used in

1 is a perspective view and a partially enlarged view of mixed fiber yarn according to an embodiment of the present invention;
2 is a perspective view of a mixed filament yarn for cell culture support according to an embodiment of the present invention;
3a and 3b are various examples of first yarns constituting mixed filament yarns according to an embodiment of the present invention, and FIG. 3a is a perspective view of a plurality of multifilament first yarns implemented in a twisted form, and FIG. 3b is a perspective view showing that a part of the twisted first yarn of FIG. 3a is partially untwisted to include spaced apart spaces between the strands constituting the first yarn;
4 is a cross-sectional view of a mixed filament yarn for a cell culture support according to an embodiment of the present invention;
5A to 5C are SEM images of mixed fiber yarns for cell culture supports in which a second yarn is directly electrospun on a plurality of first yarns according to an embodiment of the present invention. is × 10.0k times, FIG. 5C is a photograph magnified × 5.0k times,
6A and 6B are examples of slitting yarn included in an embodiment of the present invention. FIG. 6A is an enlarged picture of a fiber web before being made into a slitting yarn, and FIG. 6B is an enlarged picture after being made of a slitting yarn.
7 is a photograph showing an intermediate step for manufacturing a slitting yarn included in an embodiment of the present invention, FIG. 7 (a) is a photograph of a slitting yarn primarily slit to a width of 50 mm, and FIG. b) is a photograph showing the process of precisely slitting the primary slitted yarn into a width of 1.5 mm, and FIG. 7(c) shows the slitting yarn having a width of 1.5 mm manufactured in FIG. photos showing the process
8 is a view showing images taken through a confocal microscope by height from the bottom to the top of the specimen in a state in which the cell aggregates are attached to the cell aggregate specimen cultured in the slitting yarn according to an embodiment of the present invention, and
9 is a SEM picture of a first yarn included in an embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. This invention may be embodied in many different forms and is not limited to the embodiments set forth herein. In order to clearly describe the present invention in the drawings, parts irrelevant to the description are omitted, and the same reference numerals are added to the same or similar components throughout the specification. In addition, the dimensions of each component in the drawing may be appropriately changed in order to clearly understand an embodiment of the present invention. For example, in FIG. 4, the diameter of the spun nanofibers 311 forming the surface portion is Note that it may be the same as the diameter of the nanofiber 312 .

Referring to FIG. 1, the mixed filament yarn 200 for a cell culture support according to an embodiment of the present invention has a first yarn 220 having a support function and at least a portion of the outer surface of the first yarn 220. It is implemented by including the second yarn 210 to be covered. In addition, the second yarn 210 includes nanofibers 211 having an average diameter of 1000 nm or less.

Although FIG. 1 shows that one strand of second yarn 210 covers the outer circumference of one strand of first yarn 220, it is not limited thereto, and as shown in FIG. 2, two or more first yarns 210 are two or more first yarns. (220') may be coated on the outer surface. In addition, it should be noted that the second yarn may cover the outside of one or more first yarns with multiple strands instead of one strand.

At this time, the coating may be implemented by covering the outer surface of the first yarn in a manner in which the second yarn is wound, but is not limited thereto. In addition, the second yarn 210 is shown as being coated on the entire surface except for the front end and end of the first yarn 220, but is not limited thereto, and is covered on the entire surface including the front end and end of the first yarn 220. Or it may be coated only on a portion of the first yarn (220).

On the other hand, when cells are cultured on a support formed of nanofibers in consideration of the size of the cells, it is difficult for the cells to be stably attached, and even after being attached, they are easily detached from the support due to the fluid flow of the culture medium applied to the support, the surrounding vibration, etc. Cultivation and proliferation rates may be reduced. In addition, cells attached to the surface of the nanofibers due to the flow of the nanofibers may form non-uniform clusters, and it is difficult to differentiate and recover the cells. Furthermore, in the case of nanofibers spun by including functional materials in a spinning solution for producing nanofibers for efficient cultivation of cells, there is a concern that mechanical strength may be lowered due to compatibility problems between functional materials and components forming nanofibers. Therefore, during cell culture, the nanofibers may be rejected or the support formed including the nanofibers may collapse, so that it may not be possible to provide a stable environment as a support for cell culture. In addition, when a fabric embodied by weaving or knitting is used as a cell culture support rather than nanofiber itself, nanofiber has a problem in that it is difficult to retain sufficient mechanical strength to perform the weaving or knitting process.

Accordingly, in the mixed filament yarn 200 for cell culture support according to the present invention, the first yarn 220 having a support function is plied with the second yarn 210 to supplement the mechanical strength of the second yarn 210, which is a nanofiber. And, preferably, the second yarn 210 is disposed outside the first yarn 220 such that the first yarn 220 is a core yarn, so that the cells to be cultured are in the second yarn 210 including nanofibers By enabling contact and culture, the efficiency of cell culture can be improved and at the same time, cells can be stably cultured.

In addition, through this arrangement structure, it is possible to increase the degree of adhesion of the second yarn 210 to prevent slipping and improve tensile strength and durability. That is, despite the fact that the second yarn 210 is composed of nanofibers considering the size of cells according to an example of the present invention, the second yarn 210 is twisted or covered with the first yarn 220 so that smooth stretching action is achieved. Since this can happen, the cutting rate or breakage rate of the second yarn 210 can be greatly reduced.

As described above, the first yarn 220 is a yarn that performs a support function by securing the mechanical strength of the second yarn 210 including nanofibers to be described in detail below.

The first yarn 220 may be a yarn having a commonly known fiber material, and as a non-limiting example thereof, the fiber forming component is polystyrene (PS), polyester, polyethersulfone (PES), polyvinyl Leaden fluoride (PVDF), polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), polyamide, polyalkylene, poly(alkylene oxide), polyurethane, polyamino acid (poly( amino acids)), poly(allylamines), polyphosphazene, and polyethylene oxide-polypropylene oxide block copolymer, or any one or more non-biodegradable components selected from the group consisting of polycaprolactone, Polydioxanone, polyglycolic acid, poly(L-lactide) (PLLA), poly(DL-lactide-co-glycolide (PLGA)), polylactic acid and polyvinyl It may contain one or more biodegradable components selected from the group consisting of alcohol (polyvinyl alcohol).

In addition, as shown in FIGS. 1 and 2 , the first yarns 220 and 220 ′ may be single-stranded single yarns as shown in FIG. 1 or multi-yarns having multiple mono-yarns as shown in FIG. 2 . At this time, the first yarn may be multi-yarn so that the second yarn covering the outside of the first yarn has a more improved specific surface area and surface morphology, more preferably twisted or twisted as shown in FIGS. 3a and 3b. After that, it may be in the form of having a space separated from each other by being partially released. In this case, the curvature of the outer surface of the first yarns 220'A and 220'B is significantly increased, or the cell cultured due to the increase in curvature that can be expressed due to the spaced gap between the monofilament together with the curvature of the outer surface There is an advantage that their adhesion and culture efficiency can be remarkably improved.

In addition, the average diameter of the first yarns 220 and 220' may be 100 to 1000 μm. In this case, when the first yarn is a multi yarn including a plurality of mono yarns, the average diameter of the mono yarn may be 50 to 500 μm, and the number of strands may be 2 to 15, but is not limited thereto.

Next, the second yarn 210 covering the outside of the above-described first yarns 220, 220', 220'A, and 220'B will be described.

The second yarn 210 is a fiber that the cells to be cultured directly contact through the mixed filament yarn 200, and the diameter can be determined in consideration of the type and size of the cells to be cultured, but the average diameter is 1000 nm or less. It includes fibers, and may preferably be 100 to 900 nm. When the average diameter of the nanofibers exceeds 1000 nm, it may be difficult to prepare a three-dimensional cell cluster at a desired size or the cell culture rate may decrease due to a decrease in the specific surface area to which cells are attached. On the other hand, the average diameter of the nanofibers may be 100 nm or more. If the average diameter is less than 100 nm, the productivity is reduced due to frequent yarn cutting during the manufacture of the second yarn, and the second yarn is attached to the outside of the first yarn due to weak mechanical strength. It may be difficult to place the yarn, and it may be difficult to stably culture the cells even during cell culture.

In addition, the second yarn may be implemented with a known fiber-forming component that can be manufactured including nanofibers, and since the material can be selected differently in consideration of a predetermined purpose such as degradability, the present invention relates to this. Not particularly limited. The fiber-forming component may include a natural fiber component such as a cellulose component such as cotton or hemp, a protein component such as wool or silk, or a mineral component. Alternatively, the fiber-forming component 110 may be a component of a known artificial fiber.

On the other hand, the fiber-forming component forming the second yarn is polystyrene (PS), polyester, polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), poly Dimethylsiloxane (PDMS), polyamide, polyalkylene, poly(alkylene oxide), polyurethane, poly(amino acids), poly(allylamines), polyphosphazene (polyphosphazene) and any one or more non-biodegradable ingredients selected from the group consisting of polyethylene oxide-polypropylene oxide block copolymer, or polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly(L-lactide)), PLGA (poly(DL-lactide-co-glycolide)), polylactic acid, and polyvinyl alcohol. can include

In addition, referring to FIG. 1, the second yarn 210 may be a slitting yarn obtained by cutting a nanofiber web having a three-dimensional network structure formed of nanofibers 211 to a predetermined width, and the second yarn, which is the slitting yarn 210 covers the outside of the first yarn 220, so that the mixed yarn 200 can be implemented. At this time, the slitting yarn may be provided with one strand or multiple strands to form the second yarn.

The slitting yarn may be manufactured by cutting a sheet-shaped fiber assembly, fabric, or the like to have a predetermined width. Preferably, the slitting yarn may be a sheet-like nanofiber web having a three-dimensional network structure cut to a predetermined width. At this time, the cutting process is facilitated when the fiber web is pressed with a certain pressure before cutting and then cut. , there is an advantage of increasing the strength of the slitting yarn. For example, when the sheet-like nanofibrous web having a three-dimensional network structure of FIG. 6a is compressed and cut to a predetermined width, a slitting yarn as shown in FIG. 6b can be manufactured. When the slitting yarn formed of the nanofiber web is the second yarn, the culture solution can move between the outside and the inside of the second yarn, so that the cells can be cultured more smoothly. In addition, since cells can move and proliferate not only on the surface of the nanofiber web, but also inside it, it may be more advantageous to implement a three-dimensional cell cluster.

However, even in the case of a nanofiber web designed to have numerous flow channels, when compressed to be realized as a slitting yarn, the flow path decreases and the average pore diameter also decreases, so that the culture solution does not pass through to the desired level, and cells move and proliferate into the pores In this case, it may be difficult to culture 3-dimensional cell clusters. Accordingly, the nanofiber web of the slitting yarn may have a basis weight of 0.1 to 100 g/m2, more preferably 3 to 50 g/m2, and more preferably 7 to 30 g/m2. If the basis weight is less than 0.1 g / m 2, the handling property is poor and the slitting process tends to be unstable and not easy, so there is a risk of reduced productivity due to frequent trimming. In addition, when the basis weight exceeds 100 g / m 2, the compression of the nanofibrous web is large, so the pore diameter and flow path inside the nanofibrous web are significantly reduced, and the cross-sectional shape of the support fibers forming the nanofibrous web is pressed into an oval shape. In this case, there is a problem in that the morphology of the surface of the nanofiber web or the inner empty space may be changed, making it difficult for the cells to culture and grow in three dimensions.

In addition, the thickness of the slitting yarn may be 7 to 30 μm, more preferably 9 to 25 μm. If the thickness of the slitting yarn 200 is less than 7 μm, handling is deteriorated, mechanical strength is weak, and there is a risk of thread breakage in the twisting process performed after single or multiple strands are plied, and it is difficult to stably cultivate cells can In addition, if the thickness is reduced due to excessive compression, the porosity and average pore size may decrease, and the surface morphology may become flat, making it difficult to stably culture the cells. In addition, if the thickness exceeds 30㎛, it can be considered differently depending on the degree of basis weight, but if the compression is less, the surface morphology is not stable, so cells may be detached frequently during culture, and in a sufficiently compressed state, the thickness The thick case may also be accompanied by a decrease in porosity and average pore size, and this may make it difficult to culture cells in three dimensions due to a decrease in the permeability of the cell culture medium and a decrease in the internal penetration of cells.

In addition, the ratio of the basis weight to the thickness of the slitting yarn (basic weight (g / ㎡) ÷ thickness (㎛)) may be 1: 0.8 to 1.7, more preferably 1: 1 to 1.25, through which the slitting of cells There is an advantage in that three-dimensional cell aggregates can be more stably cultured due to easy penetration into the Tingsa, and that the cell culture solution can be permeated smoothly to minimize death of the cells loaded therein. If the ratio of basis weight to thickness is less than 0.8, the surface morphology is not stable, and cells may be frequently detached during culture. Therefore, it may be difficult to culture cells in three dimensions due to a decrease in the permeability of the cell culture medium and a decrease in the internal penetration of cells. In addition, if the ratio of basis weight to thickness exceeds 1.7, the compression process may be performed excessively and the thickness may be thinned, which may result in a decrease in porosity and average pore diameter, and flat surface morphology. Therefore, it may be difficult to stably culture the cells.

In addition, the slitting yarn may have a width of 0.1 to 8 mm, and if the slit width is less than 0.1 mm, cutting is not easy and frequent cutting may be induced. In addition, there is a problem that can be easily cut by tension and rotational force applied when a plurality of slitting yarns are twisted. In addition, when slitting with a width exceeding 30 mm, it may be difficult to achieve desired effects, such as uneven twisting, which may occur in a twisting process of manufacturing yarn by plying a plurality of slitting yarns with the first yarn.

In addition, the average pore diameter of the nanofiber web forming the slitting yarn is preferably provided to secure enough space for cells to move and proliferate in the pores inside, and the diameter can be determined according to the specific type of cells to be cultured can For example, the average pore diameter may be preferably 0.05 to 10 μm, more preferably 10 nm to 1 μm. If the average pore diameter is less than 0.05 μm, when the cultured cells proliferate, they can move and proliferate in a planar way through the outer surface of the nanofibrous web rather than moving to the pores inside the nanofibrous web, and thus a cell group having a three-dimensional shape at a desired level. Collectives may not be able to be cultured. In addition, even if the cells move into the inner space of the nanofiber web, the culture solution may not smoothly pass through the nanofiber web, so there is a problem that the cells that have moved into the inner space may die or their proliferation may be reduced. In addition, if the average pore diameter exceeds 10 μm, the movement of cells into the inner space of the nanofiber web and the passage of the culture solution may be good, but the cells to be cultured escape out of the slitting yarn together with the culture solution passing through the nanofiber web The phenomenon may be increased, and the increase in isolated cultured cells is difficult to culture into a cell aggregate having a desired three-dimensional shape.

The nanofibers, which are support fibers forming the slitting yarn nanofiber web, may have an average diameter of 10 nm to 1000 nm or less, preferably 100 nm to 900 nm. If the average diameter of the support fibers is less than 10 nm, the mechanical strength of the fiber web may be significantly reduced, and if the average diameter exceeds 1000 nm, it is difficult to manufacture a nanofiber web having a desired level of porosity and specific surface area. can

On the other hand, in order to culture cells in three dimensions, cells penetrate and culture not only the surface of the nanofibrous web but also the inner space, and eventually penetrate into the inner space and contact between the cultured cells and the cells cultured on the surface to form a single three-dimensional Cell clusters can be formed. However, even in the case of cells cultured on the surface, three-dimensional culture of cells can be induced by the surface morphology of the nanofiber web. As an example of this, the surface roughness may be large so that the morphology of the surface of the nanofiber web implemented by the slitting yarn is not flat, but is bumpy. The rugged surface shape of the nanofiber web is illustratively including a plurality of concave and/or convex portions, and in addition to the three-dimensional growth effect of cells, the cells are more easily and firmly seated in the space between the convex portions or in the grooves of the concave portions. Therefore, there is an advantage in that the number of detached cells after loading on the slitting yarn can be significantly reduced.

According to a preferred embodiment of the present invention in order to realize a yarn having a morphological surface for three-dimensional growth of cells and improvement of stability of seeded cells, the nanofiber web of the slitting yarn includes a plurality of support fibers And, the diameter dispersion coefficient (E) of the support fibers may be 8 to 25%. The diameter dispersion coefficient (E) is a parameter that can estimate how closely or widely the support fibers are distributed compared to a predetermined average diameter calculated from the distribution chart based on the diameter of the support fibers, Calculated by Equation 1 below It can be.

[Equation 1]

A diameter dispersion coefficient (%) of 0% according to Equation 1 means that the standard deviation is 0, which means that the diameters of the plurality of support fibers included in the fiber web all match the average diameter. Therefore, on the contrary, the fact that the diameter dispersion coefficient gradually increases means that the number of support fibers having a larger diameter and/or a smaller diameter than the average diameter among the plurality of support fibers included in the fiber web increases.

As the support according to one embodiment of the present invention satisfies a predetermined average diameter and the dispersion coefficient for the diameter of the support fibers satisfies 8 to 25%, as described above, a plurality of concave and/or convex portions are arranged. It may be easier than realizing a nanofibrous web having a rugged surface morphology. However, if the dispersion coefficient is excessively large, the increase in basis weight relative to the thickness may be large, and this may greatly reduce the average pore diameter and air permeability, and it may be difficult to introduce or exchange the cell culture medium into the compressed nanofiber web. Cell culture may be difficult inside the nanofiber web, which may reduce cell culture efficiency. If the dispersion coefficient for the diameter is less than 8%, it is advantageous to develop a smooth surface morphology as the uniformity of the diameter of the support fibers increases, and the uniformity of the pores also increases, but the seeded cells do not proliferate in three dimensions, but rather on the surface. If cultured two-dimensionally or if the average diameter of the support fibers is large, a pore structure with a large average pore diameter is generated, and the seeded cells may be detached. In addition, if the dispersion coefficient for the diameter exceeds 25%, the average pore diameter of the nanofibrous web becomes very small as the non-uniformity with respect to the diameter of the support increases in the nanofibrous web having a rather small average diameter, and this causes the nanofibers of the cell culture medium. Inflow or exchange into the inside of the web may become difficult, and there is a concern that cells may be cultured along the surface rather than being cultured in three dimensions.

In addition, the air permeability of the nanofiber web may be 1 to 10 cfm. One of the important factors when growing cells three-dimensionally on slitting yarn, which is an example of the second yarn, is whether materials necessary for cell culture can be continuously and smoothly supplied. If cells grow three-dimensionally on the surface of the slitting yarn, cells placed adjacent to the surface of the slitting yarn or cells that are immersed or settled in the inner space of the slitting yarn and cultured are cell culture medium compared to cells positioned to be exposed to the external space. It may be difficult to get in touch smoothly. Therefore, the air permeability of the nanofibrous web may be 1 to 10 cfm for smooth inflow and outflow of the cell culture medium into and out of the compressed nanofibrous web. If the air permeability is less than 1.0 cfm, it may be difficult for cells to penetrate the inside of the support, and the passage of components capable of dissolving the cell culture medium or biodegradable components may not be smooth. In addition, if the air permeability exceeds 10 cfm, since it is implemented as a slitting yarn, the mechanical strength of the nanofiber web is significantly low or the diameter and thickness of the fineness of the support fiber are significantly large. It may be difficult to apply to an incubator having a limited and small volume, and it may be difficult to culture in a desired amount due to detachment of the seeded cells.

In addition, the nanofibrous web may have a porosity of 40 to 90%, and through this, it is easier to form a three-dimensional cell cluster through cells moving and proliferating inside the nanofibrous web, and the pores inside the nanofibrous web There is an advantage of supporting the culture solution or improving the permeability of the culture solution. If the porosity is less than 40%, it is difficult to form a three-dimensional cell aggregate, and there is a problem that may cause the death of cells moving and proliferating inside the nanofiber web. In addition, if the porosity exceeds 90%, there is a problem that the scaffold may collapse during cell culture due to weakening of mechanical strength of the scaffold.

In addition, when the slitting yarn is the second yarn, the first yarn and the second yarn have a weight ratio of 1: 0.5 to 10, more preferably a weight ratio of 1: 1 to 5, more preferably a weight ratio of 1: 1 to 2 may be provided. If the weight ratio of the second yarn to the weight of the first yarn is less than 0.5, it may be difficult to implement a three-dimensional cell cluster due to a decrease in the specific surface area of the mixed yarn. In addition, if the weight ratio of the second yarn to the weight of the first yarn is implemented in excess of 10, it may be difficult to improve the mechanical strength to the desired level, and the cell culture medium under conditions where flow or vibration is applied It may be difficult to cultivate stably, and it may be difficult to weave or knit into fabric.

When the slitting yarn is the second yarn, the mixed yarn may be twisted so that the second yarn 210, which is the slitting yarn, surrounds the first yarn 220. In this case, there is an advantage in that the surface area to which cells can be attached is widened, making it easy to culture cells constituting a colony and simultaneously culturing many cells. In this case, the degree of twisting the second yarn 210 to the first yarn 220 may be determined in consideration of the type and size of the cells to be cultured, and the shape and size of the cell aggregate. For example, the second yarn 210 may have soft water of 10 to 100,000 T/cm. If the soft water is less than 10T/cm, the shaking of the second yarn due to the external force such as the flowing cell culture medium is large, and when the second yarn is provided with multiple strands of mono yarn, additional shaking according to the distance between the mono yarns Due to this, there is a problem that it may be difficult for the cells to be cultured stably. In addition, if the number of soft fibers exceeds 100,000T/cm, the strength of the second yarn or the mixed yarn in which the second yarn and the first yarn are plied may rather decrease due to the friction between the mono yarns or the mono yarns, and due to excessive twisting There may be a problem in that the volume of the trabecular bone decreases due to the degree of adhesion between the kites and the kites.

On the other hand, according to another embodiment of the present invention, as shown in FIGS. 4 and 5A to 5C, the mixed yarn 300 has a second yarn 310 directly attached to the outer surface of the first yarn 320. It may be realized by spinning, and specifically, the second yarn 310 may be a nanofiber assembly formed of electrospun nanofibers 311 and 312. In this case, a nanofiber web having a three-dimensional network structure can be formed through the directly spun nanofibers being folded and arranged/stacked multiple times without determining the direction. Alternatively, a nanofiber web having a three-dimensional network structure may be formed by independently folding a plurality of nanofibers and/or arranging/stacking each nanofiber without determining the fiber length direction. At this time, bonding may occur between different surfaces within one strand of nanofibers and/or between surfaces of different nanofibers, and through this, the 3D network structure may be structurally more complex. In this case, the cells loaded on the scaffold can migrate/proliferate into pores formed in a three-dimensional network structure, and it may be advantageous to culture the cells in a cell cluster having a three-dimensional shape/structure. In addition, in order to increase the growth rate and survival rate of cells cultured inside/outside the support, it is important to supply nutrients necessary for cell growth. As a flow path is formed, nutrients can be easily supplied to the cells located inside the scaffold, through which cell death can be prevented and cell proliferation can be improved.

On the other hand, according to the amount of nanofibers spun, the nanofiber assembly has a surface portion where the nanofibers (311.312) cover the outer surface of the first yarn, and a portion of the nanofibers extending from the surface portion toward the outside. It may include protruding protrusions. At this time, as the surface portion is formed by the nanofibers 311 disposed in contact with the outside of the first yarn 320, the outer surface of the surface portion has a surface formed with numerous curves due to the outer surface of the nanofibers 311. However, these bends can increase the specific surface area of the scaffold to enable smooth attachment and culture of cells and have a surface morphology more suitable for cell culture. In addition, the nanofibers 312 of the protrusions also increase the specific surface area of the second yarn and enable various surface morphologies to be implemented.

At this time, in order to improve adhesion between the surface portions of the first yarns, some of the nanofibers 311 of the surface portions may be partially or wholly bonded to each other to cover the outside of the first yarns. If the surface portion is not formed in a predetermined area and individual nanofibers are provided on the surface of the first yarn, it is difficult to have a sufficiently large specific surface area, and the individual nanofibers are formed by external force, that is, the flow or vibration of the culture solution. As it is easily shaken, it may be difficult to stably culture the loaded cells, and in some cases, there is a concern that the cells may be detached from the support.

On the other hand, in the embodiment as shown in Figure 4, the first yarn may be provided with a multi-yarn having a plurality of mono yarns as shown in Figures 5a to 5b, through which the adhesion between the nanofiber assembly and the first yarn can be further improved. and stable cell culture may be possible. More preferably, as shown in FIG. 3B, the first yarn may be partially twisted after being twisted with multi-yarns, and through this, greater adhesion is expressed due to an increase in the specific surface area that can be bonded with the nanofibers forming the nanofiber assembly. There are benefits you can do.

In addition, it is preferable that the thickness of the surface portion is 0.2 to 5 μm. If the thickness exceeds 5 μm, there is a concern that the curved surface morphology of the surface portion may decrease and the specific surface area may decrease, which may reduce cell culture efficiency. 3 Cultivation of 3D cell populations can be difficult. In addition, if the thickness is less than 0.2 μm, there is a fear that the adhesion with the first yarn may be reduced, and as a result, the flow of the second yarn may be intensified, making it difficult for cells to be stably expressed. In addition, it may be difficult for cells to move inside the surface portion, and thus, it may be difficult to culture them into a three-dimensional cell cluster.

In addition, when the nanofiber assembly is a second yarn, the first yarn and the second yarn have a weight ratio of 1: 2 to 5, more preferably a weight ratio of 1: 1 to 3, more preferably a weight ratio of 1: 1 to 2 can be provided with If the weight ratio of the second yarn to the weight of the first yarn is less than 2, it may be difficult to implement a three-dimensional cell cluster due to a decrease in the specific surface area of the mixed yarn. In addition, even if the weight ratio of the second yarn compared to the weight of the first yarn is implemented in excess of 5, it may be difficult to sufficiently improve the specific surface area due to severe accumulation and bonding between nanofibers on the outer surface of the mixed filament yarn, and it may be difficult to sufficiently improve the specific surface area. It may be difficult to move to the inside of the unit, making it difficult to culture into a three-dimensional cell aggregate. In addition, it may be difficult for cells to be stably cultured under conditions where flow or vibration of the cell culture medium is applied, and it may be difficult to weave or knit into a fabric.

When the second yarn is a nanofiber assembly, the mixed yarn can be implemented by electrospinning nanofibers on the outside of the first yarn. At this time, the electrospinning is, for example, spinning the nanofibers from a plurality of nozzles in one direction while twisting the first yarn. Alternatively, the nozzle moves and spins at 360 ° around the first yarn, or it can be implemented by radiating nanoislands from a plurality of nozzles that are fixedly arranged by dividing 360 ° around the first yarn at a predetermined angle. . The specific method is not limited thereto, and may be implemented through appropriate changes using an electrospinning device possessed.

Meanwhile, the above-described second yarns 210 and 310 may include a physiologically active component that induces any one or more of cell attachment, migration, growth, proliferation, and differentiation.

The bioactive substance may be a substance that can help in culturing cells or recovering cultured cells.

The physiologically active component may be provided by being coated on the outside of the nanofibers included in the second yarn, or may be provided by spinning in a mixed state in the preparation step of the spinning solution forming the nanofibers.

The physiologically active component may be, for example, an adhesive component, through which the cultured cells are initially fixed on the cell support to prevent the cells loaded on the culture solution from floating and to prevent the cells from being detached from the support during cultivation. can be performed. The adhesive component may be used without limitation in the case of a known adhesive component that has normal biocompatibility and does not cause cytotoxicity, but preferably consists of repeating the amino acid sequence of SEQ ID NO: 1 to SEQ ID NO: 7 1 to 20 times. It may contain at least one selected from the group consisting of proteins and proteins in which at least two of these proteins are fused, and through this, cytotoxicity is remarkably lowered, adhesiveness is excellent, and adhesive components are dissolved in the culture solution during cell culture. There is an advantage in that the detachment of physiologically active components and the isolation of cells can be prevented.

In addition, the physiologically active component directly or indirectly induces any one or more of cell attachment, migration, growth, proliferation, and differentiation so as to promote cell culture in addition to attaching cells to the support. It may be a material, and if it is a known material known to express such a function, it may be included without limitation. For example, the physiologically active ingredient is monoamine, amino acid, peptide, saccharide, lipid, protein, glycoprotein, glycolipid, proteoglycan, mucopolysaccharide, and nucleic acid. acid) may include any one or more compounds selected from the group consisting of cells and any one or more. In this case, monoamines include, for example, compounds containing primary amines such as catecholamine and indolamine. In addition, the peptide includes an oligopeptide, and the protein includes a polypeptide and may be, for example, fibronectin. The sugars include monosaccharides, polysaccharides, oligosaccharides, and carbohydrates, and the lipids may be, for example, steroid hormones.

Meanwhile, the physiologically active ingredient may include a motif. The motif may be a natural peptide or a recombinant peptide comprising a predetermined amino acid sequence provided in at least one selected from proteins, glycoproteins, and proteoglycans included in growth factors or extracellular matrix. Specifically, the motif is adrenomedullin, angiopoietin, bone morphogenetic protein (BMP), brain-derived neurotrophic factor (BDNF), epidermal growth factor (EGF), erythropoietin, fibroblasts Fibroblast growth factor, glial cell line-derived neurotrophic factor (GDNF), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF) CSF), growth differentiation factor-9 (GDF9), hepatocyte growth factor (HGF), hepatoma-derived growth factor (HDGF), insulin-like growth factor , IGF), Keratinocyte growth factor (KGF), Migration-stimulating factor (MSF), Myostatin (GDF-8), Nerve growth factor (NGF), Platelet-derived growth factor (PDGF), thrombopoietin (TPO), T-cell growth factor (TCGF), neuropilin, transforming growth factor-alpha (TGF -α), transforming growth factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), IL-1, IL-2, IL-3, IL-4 , IL-5, may include a predetermined amino acid sequence included in any one or more growth factors (GF) selected from the group consisting of IL-6 and IL-7. Or, at least one extracellular matrix selected from the group consisting of hyaluronic acid, heparin sulfate, chondroitin sulfate, termatine sulfate, keratan sulfate, alginitis, fibrin, fibrinogen, collagen, elastin, fibronectin, vitronectin, cadherin, and laminin. It may include a sequence of predetermined amino acids included in (extracellular matrix). In addition, the motif may include both a predetermined amino acid sequence included in the growth factor and a predetermined amino acid sequence included in the extracellular matrix. More preferably, the motif may include one or more selected from the group consisting of proteins comprising the amino acid sequences of SEQ ID NO: 8 to SEQ ID NO: 28 and proteins in which at least two of these proteins are fused, but are not limited thereto no.

On the other hand, the motif may be integrally implemented by covalently bonding to the above-described adhesive component. For example, when the adhesive component is a protein, the motif may be covalently bonded directly to the N-terminus and/or C-terminus of the polypeptide or covalently bonded through a heterogeneous peptide or polypeptide, in which case the second yarn It is possible to more firmly attach the physiologically active component to the nanofibers, and it is possible to minimize the separation of the physiologically active component during cell culture.

In addition, the nanofibers forming the second yarn may further include a fiber cutting component. In general, after culturing cells, a process of separating the cells from the cultured scaffold is required in order to move them to a target implant or the like. At this time, the cultured cells are not easily detached from the scaffold due to cell adhesion and clustering, and damage to the cells or scaffold may occur. In particular, in the case of colony-forming cells characterized in that they are cultured together, it is not easy to recover the cells, so a culture technique or functional material for separating them from the tissue of the cell culture support is required.

Accordingly, the mixed filament yarn 200 for a cell culture support according to the present invention may further include a fiber cutting component in the second yarn in order to easily recover cells without damaging the cells cultured on the support. The fiber cutting component may include known components for cell recovery.

The fiber cutting component may be prepared by being included in a spinning solution for implementing nanofibers, and in this case, the fiber cutting component may be exposed to a portion of the surface of the nanofibers, and some of them may also be placed inside the nanofibers. there is. The fiber cutting component allows the nanofiber itself to be cut around the fiber cutting component so that the cell cluster, which is a cultured product, can be easily separated from the mixed filament yarn. For example, the fiber cutting component imparts a predetermined stimulus to the nanofibers and changes the surface of the nanofibers to non-adhesive cells, thereby separating the cultured cells and the mixed filaments. Alternatively, as the fiber cutting component is removed from the fiber by a method such as dissolution, the cell cluster can be separated by directly cutting the nanofiber.

The mixed filament yarn for cell culture support according to the present invention further comprising such a fiber cutting component can release cells from the support without damaging the cultured cell population and support, so that cell recovery can be increased.

On the other hand, the present invention can implement a fabric for a cell culture support comprising the mixed filament yarn according to the present invention described above.

The fabric may be any one of a woven fabric, a knitted fabric, and a non-woven fabric, and may be manufactured in a different form depending on the purpose. The fabrics, knitted fabrics and nonwoven fabrics may be manufactured through known implementation methods. For example, the fabric may be a twill fabric woven by including one or more of warp and weft yarns by combining the above-mentioned mixed yarns alone or by piling them. In addition, as an example, the knitted fabric may be a flat knitted weft knitted by introducing the above-mentioned mixed yarn alone or plied into a weft knitting machine. In addition, as an example, the nonwoven fabric may be manufactured by adding an adhesive component to a short-cut yarn obtained by cutting the above-described blended yarn at a constant fiber length using heat/pressure.

In addition, it is possible to implement an implant for tissue engineering including cultured cells by transplanting cultured cells to the fabric described above according to the present invention. At this time, the cultured cells may be cultured on the outer surface of the first and second yarns of the mixed filament yarn or between the spaced spaces between the mixed filament yarns.

At this time, the cells are pluripotent stem cells, pluripotent stem cells, pluripotent stem cells, oligopotent (oligopotent) stem cells, and any one or more stem cells selected from the group consisting of single stem cells, and hematopoietic stem cells, hepatocytes, fiber cells, epithelial Cells, mesothelial cells, endothelial cells, muscle cells, nerve cells, immune cells, adipocytes, chondrocytes, bone cells, blood cells and skin cells may include at least one type of differentiated cells selected from the group consisting of. For example, the cell may be a cell having a shape elongated in one direction rather than a spherical shape or a cell with strong mobility.

sequence number amino acid sequence One Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser TyrPro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser TyrPro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu
Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser
Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys
Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly
Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr
Lys Lys Tyr Tyr Gly Gly Ser Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser
Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys
Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro
Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser TyrPro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala
Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly
Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn
Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala
Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro
Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro
Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr
Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly GlyGly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp
Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr HisTyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr
Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys
Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg
Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr ProPro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys
Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr
Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 8 Arg Gly Asp 9 Arg Gly Asp Ser 10 Arg Gly Asp Cys 11 Arg Gly Asp Val 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro 13 Gly Arg Gly Asp Ser 14 Gly Arg Gly Asp Thr Pro 15 Gly Arg Gly Asp Ser Pro 16 Gly Arg Gly Asp Ser Pro Cys 17 Tyr Arg Gly Asp Ser 18 Ser Pro Pro Arg Arg Ala Arg Val Thr 19 Trp Gln Pro Pro Arg Ala Arg Ile 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe 23 Ile Lys Val Ala Asn 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln 25 Val Ala Glu Ile Asp Gly Ile Gly Leu 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys

<Example 1>

As the first raw yarn, a PET multifilament yarn of 6 strands (monosa diameter of about 15 µm) having a total diameter of 50 µm was prepared. In addition, a slitting yarn was prepared as a second yarn. Specifically, the slitting yarn is first, 12g of polyvinylidene fluoride (Arkema, Kynar761) as a fiber forming component to prepare a spinning solution, dimethylacetamide and acetone in a weight ratio of 70: 30 Mixed solvent 88g to 80 A mixed solution was prepared by dissolving using a magnetic bar at a temperature of ° C. for 6 hours. The prepared spinning solution was electrospun using an electrospinning device in an environment of RH 65% and 30 ° C under the conditions of an applied voltage of 25KV, a distance between the current collector and the spinneret of 25cm, and a discharge amount of 0.05ml / hole. A nanofibrous web having a diameter of 402 nm, a thickness of 12.5 μm, and a basis weight of 8 g/m 2 was obtained. The obtained nanofiber web was calendered twice at a temperature of 130 ° C. and a pressure of 4 kPa to produce a nanofiber web having a three-dimensional network structure, a basis weight of 10 g / m 2 and a thickness of 10 μm, as shown in FIG. , After the first cutting process with a width of 50 mm in FIG. 7 (a) and the precise cutting process with a width of 1.5 mm in FIG. 7 (b), the manufactured nanofiber web is wound around a roll as shown in FIG. A second yarn was produced.

Then, the first yarn and the second yarn are twisted together so that the first yarn and the second yarn have a weight ratio of 1: 2 so that the second yarn covers the outside of the first yarn using the first yarn as a core yarn, and the following Table 2 and The same mixed yarn was prepared.

<Examples 2 to 7>

It is manufactured in the same manner as the slitting yarn prepared in Example 1, but by adjusting the basis weight / thickness of the nanofiber web by varying the compression strength, the nanofiber web is prepared to have basis weight and thickness as shown in Table 2 below. Mixed filament yarns for cell scaffolds as shown in Table 2 below were prepared using Ting yarn as a second yarn.

<Comparative Example 1>

Without the second yarn, the first yarn prepared in Example 1 was prepared as a yarn for a cell culture support.

<Experimental Example 1>

The diameter distribution of the support fibers in the slitting yarn nanofiber web used in the manufacturing process according to Examples 1 to 7 was measured.

Specifically, the average diameter and standard deviation of the diameter were calculated using the diameter distribution of the support fibers measured by the fiber diameter distribution program (developed by AMOGREENTECH), and the average diameter and dispersion coefficient are shown in Table 2.

<Experimental Example 2>

The air permeability of the slitting yarn nanofiber webs used in the manufacturing process of Examples 1 to 7 was measured and shown in Table 2 below.

The air permeability was measured by cutting the fiber web into a test area of 38 cm2 using TEXTEST instruments equipment, holding it in the equipment, and blowing air at a test pressure of 125 Pa to measure the amount of air passing through. The unit of air permeability was cfm ( ft ³ /ft ² /min).

<Experimental Example 3>

The mixed filament yarns prepared in Examples 1 to 7 and the first yarn according to Comparative Example 1 were cut to the same length and fixed to a well plate for cell culture. After loading the fibroblasts (HS27) on the prepared well plate, they were grown in 10% complete medium at 37°C for 4 days. At this time, the 10% complete medium was mixed with Dubecos' modified Eagle's medium (DMEM) and Ham's F12 medium at a volume ratio of 1: 1.5, followed by fetal bovine serum 7 vol% and penicillin 65 U/mL and streptomycin 65 μg/mL.

Then, DAPI staining was performed on the proliferated fibroblasts, and the cultured cells were attached through a confocal microscope, while varying the height in the Z-axis direction from the bottom to the top of the slitting yarn, which is the second yarn among the mixed filaments, while changing the surface and interior Photographs were taken, and the number of cells cultured on the surface of the slitting yarn, the second yarn (the average value of the number of cells on the upper surface and the number of cells on the lower surface) and the number of cells cultured inside the slitting yarn, the second yarn (cross section of the slitting yarn) The average value of the number of cells measured at 1/3 and 2/3 points of the thickness) was measured and calculated. At this time, Table 2 below shows the relative number of cells by position in other examples based on the number of surface cells and the number of internal cells measured in Example 1 as 100%, respectively. In addition, a photograph measured for each specimen cross-sectional height for Example 1 is shown in FIG. 8.

On the other hand, in the case of Comparative Example 1, the surface and interior pictures were taken while varying the height in the Z-axis direction from the bottom to the top of the first yarn, and as a result, it was confirmed that cells were cultured only on the surface, and the number of cultured cells It was shown as a relative value based on the surface cell number of Example 1.

Specifically, in FIG. 8 , it can be seen that cells cultured at a height of 7.6 μm from the lower side of the specimen to the upper side are observed and cultured to form a plurality of cell clusters at a height of 15.3 μm. In addition, it can be expected that the height of the lower surface of the slitting yarn exists between 15.3 μm and 22.9 μm, as the nanofibrous web formed of the cultured cell aggregates and support fibers is observed at a height of 22.9 μm. In addition, it can be confirmed that a large amount of cell aggregates are cultured even at a height of 38.5 μm, and a large amount of cell aggregates are cultured even at heights of 38.2 μm and 45.8 μm, which are higher than the upper surface of the slitting yarn. Through this, the slitting included in Example 1 It can be confirmed that the cells are cultured three-dimensionally evenly throughout the inside and surface of the yarn.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Comparative Example 1 Slitting sign nano fiber web support fiber average diameter (nm) 402 400 400 404 401 402 400 2nd yarn not included Support fiber dispersion coefficient (%) 13.25 13.01 14.9 12.3 15.1 14.21 13.25 Basis weight (g/㎡) 10 19 10 10 9.5 9 8 Thickness (㎛) 10 10.8 6.2 8 11.2 12.0 12.5 Basis weight (g/㎡)/thickness (㎛) One 1.76 1.61 1.25 0.85 0.75 0.64 Average pore diameter (nm) 500 430 458 472 519 538 565 Air Permeability (cfm) 2.0 1.7 1.8 1.9 2.1 2.1 2.3 Surface cell count (%) 100 73 85 93 90 78 53 25 Internal cell count (%) 100 61 80 92 83 80 55 0

As can be seen from Table 2 above,

In the case of Comparative Example 1, as the second yarn was not included, cells attached and proliferated on the outer surface of the first yarn, but compared to Example 1, it could be confirmed that the number of cells on the surface was significantly poor.

On the other hand, even when the second yarn is provided, in the case of the sling yarn of Example 7, it is difficult to stably culture the cells, such as cells being easily detached due to structural instability. It can be seen that the number of cultured cells is significantly smaller than that of Example 1. .

In addition, among the examples, mixed filament yarns including the slitting yarns according to Examples 1, 3, 4, and 5 having appropriate basis weight and thickness are three-dimensional cell clusters in preparation for mixed filament yarns including the slitting yarns according to Examples 2 and 6 It can be confirmed that it is more suitable as a support for cell culture for culturing.

<Example 8>

As the first raw yarn, a PET multifilament yarn of 6 strands (mono yarn diameter of about 15 µm) having a total diameter of 50 µm was prepared. In addition, the same spinning solution as in Example 1 was prepared in order to form the second yarn by direct spinning on the first yarn through electrospinning.

After the prepared spinning solution is twisted, 12 nozzles are positioned so that the outer surface of the first yarn, which is partially released, can be electrospun on the entire surface of 360 °, and then electrospinning so that the weight ratio to the first yarn is 1: 2.5 Mixed filament yarns in Table 3 as shown in FIGS. 5A to 5C were implemented.

<Example 9>

It was prepared in the same manner as in Example 8, but after the first yarn was twisted, some of the yarns were not twisted, and the mixed yarns in Table 3 were used as they were in the twisted state.

<Example 10>

It is manufactured in the same manner as in Example 8, but instead of the first yarn, the outer surface of one strand of mono yarn constituting the first yarn is electrospun in the same way to provide a second yarn, and then the second yarn is provided 6 strands were plied and twisted to implement the mixed fiber yarn in Table 3 below.

<Experimental Example 4>

A plain weave fabric for cell scaffolds having the same basis weight was realized by using the mixed filament yarns according to Examples 8 and 9 as weft yarns and warp yarns. After cutting the fabric to a predetermined size, it was fixed to a well plate for cell culture. After loading the fibroblasts (HS27) on the prepared well plate, they were grown in 10% complete medium at 37°C for 4 days. At this time, the 10% complete medium was mixed with Dubecos' modified Eagle's medium (DMEM) and Ham's F12 medium at a volume ratio of 1: 1.5, then fetal bovine serum 7 vol%, penicillin 65 U/mL and streptomycin 65 μg/mL.

Thereafter, DAPI staining was performed on the proliferated fibroblasts, and the number of cells cultured on the surface of the fabric with the cultured cells attached was measured and calculated through a confocal microscope. At this time, Table 3 below shows the relative number of cells on the surface of other examples based on the number of surface cells measured in Example 8 as 100%.

<Experimental Example 5>

In order to evaluate the adhesion of the second yarn, the surface of the fabric implemented in Experimental Example 4 was photographed with an optical microscope to determine whether or not the second yarn, the nanofiber assembly, on the surface within the same area of the specimen according to Examples 8 to 10 was peeled off. Observed. As a result of the observation, the exposed area was calculated compared to the surface area of the first yarn in the observation area, and it is shown in percentage in Table 3 below.

Example 8 Example 9 Example 10 honseomsa Use of first yarn, a multifilament yarn partially untwisted after twisting Use of the first yarn, a twisted multifilament yarn Plied after being electrospun in the state of mono yarn Number of cells on the surface (%) 100 91 76 Exposed area of the outer surface of the first yarn (%) 9 15 42

As can be seen from Table 3,

It can be seen that Examples 8 and 9 using multi-yarns as the first yarns have significantly better adhesion of the second yarns than Example 10, and are more suitable as cell culture supports.

200,200',300: Mixed yarn for cell culture support
210, 310: second yarn
220, 220', 220'A, 220'B, 320: first yarn

<110> AMOLIFESCIENCE <120> Blended yarn for cell culture scaffold and fabric comprising the same <130> 1064310 <150> KR 17/0087270 <151> 2017-07-10 <160> 28 <170> KopatentIn 2.0 <210> 1 <211> 196 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 1 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu 50 55 60 Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser 65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys 85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly 100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr 115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr 130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 180 185 190 Pro Thr Tyr Lys 195 <210> 2 <211> 202 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 2 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ser Ser Glu 50 55 60 Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His Tyr His Ser 65 70 75 80 Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr Lys Gly Lys 85 90 95 Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys Asn Ser Gly 100 105 110 Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg Lys Gly Tyr 115 120 125 Lys Lys Tyr Tyr Gly Gly Ser Ser Ser Ala Lys Pro Ser Tyr Pro Pro Thr 130 135 140 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser 145 150 155 160 Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 165 170 175 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 180 185 190 Pro Thr Tyr Lys Gly Arg Gly Asp Ser Pro 195 200 <210> 3 <211> 172 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 3 Met Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr 1 5 10 15 Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala 20 25 30 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 35 40 45 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Pro Trp Ala 50 55 60 Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly Gly 65 70 75 80 Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp Asn 85 90 95 Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr Gly Ser Ala 100 105 110 Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro 115 120 125 Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro 130 135 140 Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr 145 150 155 160 Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Leu 165 170 <210> 4 <211> 46 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 4 Ala Asp Tyr Tyr Gly Pro Lys Tyr Gly Pro Pro Arg Arg Tyr Gly Gly 1 5 10 15 Gly Asn Tyr Asn Arg Tyr Gly Arg Arg Tyr Gly Gly Tyr Lys Gly Trp 20 25 30 Asn Asn Gly Trp Lys Arg Gly Arg Trp Gly Arg Lys Tyr Tyr 35 40 45 <210> 5 <211> 76 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 5 Ser Ser Glu Glu Tyr Lys Gly Gly Tyr Tyr Pro Gly Asn Thr Tyr His 1 5 10 15 Tyr His Ser Gly Gly Ser Tyr His Gly Ser Gly Tyr His Gly Gly Tyr 20 25 30 Lys Gly Lys Tyr Tyr Gly Lys Ala Lys Lys Tyr Tyr Tyr Lys Tyr Lys 35 40 45 Asn Ser Gly Lys Tyr Lys Tyr Leu Lys Lys Ala Arg Lys Tyr His Arg 50 55 60 Lys Gly Tyr Lys Lys Tyr Tyr Gly Gly Gly Ser Ser 65 70 75 <210> 6 <211> 10 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 6 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 1 5 10 <210> 7 <211> 60 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 7 Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro 1 5 10 15 Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys 20 25 30 Pro Ser Tyr Pro Pro Thr Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr 35 40 45 Tyr Lys Ala Lys Pro Ser Tyr Pro Pro Thr Tyr Lys 50 55 60 <210> 8 <211> 3 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 8 Arg Gly Asp One <210> 9 <211> 4 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 9 Arg Gly Asp Ser One <210> 10 <211> 4 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 10 Arg Gly Asp Cys One <210> 11 <211> 4 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 11 Arg Gly Asp Val One <210> 12 <211> 10 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 12 Arg Gly Asp Ser Pro Ala Ser Ser Lys Pro 1 5 10 <210> 13 <211> 5 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 13 Gly Arg Gly Asp Ser 1 5 <210> 14 <211> 6 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 14 Gly Arg Gly Asp Thr Pro 1 5 <210> 15 <211> 6 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 15 Gly Arg Gly Asp Ser Pro 1 5 <210> 16 <211> 7 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 16 Gly Arg Gly Asp Ser Pro Cys 1 5 <210> 17 <211> 5 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 17 Tyr Arg Gly Asp Ser 1 5 <210> 18 <211> 9 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 18 Ser Pro Pro Arg Arg Ala Arg Val Thr 1 5 <210> 19 <211> 8 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 19 Trp Gln Pro Pro Arg Ala Arg Ile 1 5 <210> 20 <211> 12 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 20 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 1 5 10 <210> 21 <211> 12 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 21 Arg Lys Arg Leu Gln Val Gln Leu Ser Ile Arg Thr 1 5 10 <210> 22 <211> 12 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 22 Lys Ala Phe Asp Ile Thr Tyr Val Arg Leu Lys Phe 1 5 10 <210> 23 <211> 5 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 23 Ile Lys Val Ala Asn 1 5 <210> 24 <211> 16 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 24 Lys Lys Gln Arg Phe Arg His Arg Asn Arg Lys Gly Tyr Arg Ser Gln 1 5 10 15 <210> 25 <211> 9 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 25 Val Ala Glu Ile Asp Gly Ile Gly Leu 1 5 <210> 26 <211> 10 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 26 Pro His Ser Arg Asn Arg Gly Asp Ser Pro 1 5 10 <210> 27 <211> 12 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 27 Asn Arg Trp His Ser Ile Tyr Ile Thr Arg Phe Gly 1 5 10 <210> 28 <211> 12 <212> PRT <213> artificial sequence <220> <223> Motif for cell culture scaffold <400> 28 Thr Trp Tyr Lys Ile Ala Phe Gln Arg Asn Arg Lys 1 5 10

Claims (16)

A first yarn having a support function; and
A second yarn that is electrospun and coated on at least a portion of the outer surface of the first yarn and is a nanofiber assembly formed of nanofibers having an average diameter of 1000 nm or less;
In order to improve the adhesion between the nanofiber assembly and the first yarn, the first yarn is a multi-synthetic cell culture support mixed fiber yarn having a plurality of mono yarns. According to claim 1,
The first yarn is a mixed fiber yarn for cell culture support, characterized in that the average diameter is 100 ~ 1000㎛. delete delete According to claim 1,
The first yarn or the second yarn is each independently a fiber forming component polystyrene (PS), polyester, polyethersulfone (PES), polyvinylidene fluoride (PVDF), polyacrylonitrile (PAN), poly Dimethylsiloxane (PDMS), polyamide, polyalkylene, poly(alkylene oxide), polyurethane, poly(amino acids), poly(allylamines), polyphosphazene (polyphosphazene) and at least one non-biodegradable component selected from the group consisting of polyethylene oxide-polypropylene oxide block copolymer, or
Polycaprolactone, polydioxanone, polyglycolic acid, PLLA (poly(L-lactide)), PLGA (poly(DL-lactide-co-glycolide)), polylactic acid A blended yarn for cell culture support, characterized in that it contains at least one biodegradable component selected from the group consisting of (Polylactic acid) and polyvinyl alcohol. According to claim 1,
The second yarn is a mixed filament yarn for cell culture support, characterized in that it has a physiologically active component that induces any one or more of cell attachment, migration, growth, proliferation and differentiation. delete delete delete According to claim 1,
The nanofiber assembly is for a cell culture support, characterized in that the nanofibers include a surface portion covering the outer surface of the first yarn, and a protrusion extending from the surface portion and protruding a portion of the nanofibers toward the outside. Honseomsa. According to claim 10,
In order to improve the adhesion between the surface portion and the first yarn, some of the nanofibers of the surface portion are partially or entirely bonded to each other to cover the outside of the first yarn. delete According to claim 10,
The thickness of the surface portion is a mixed fiber yarn for cell culture support, characterized in that 5 ~ 10,000㎛. According to claim 1,
The first yarn and the nanofiber assembly are mixed fibers for cell culture supports, characterized in that the weight ratio is 1: 2 to 5. Claims 1, 2, 5, 6, 10, 11, 13, and 14 for a cell culture support comprising the blended yarn for a cell culture support according to any one of claims 14 to 14 fabric. The fabric for a cell culture support according to claim 15; and
An implant for tissue engineering comprising cells cultured on the fabric.

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