KR20190002366A - Pharmaceutical composition for preventing or treating comprising avocado oil as an active ingredient - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for preventing or treating hearing loss comprising avocado oil as an active ingredient. More specifically, the avocado oil, according to the present invention, in particular, avocado shell oil pressed from the shell of an avocado, significantly increases the number of hair cells reduced by kanamycin in zebrafish models, such that the avocado oil can be usefully used for the treatment of hearing loss.

Description

[0001] PHARMACEUTICAL COMPOSITION FOR PREVENTING OR TREATING COMPRISING AVOCADO OIL AS AN ACTIVE INGREDIENT [0002]

The present invention relates to a pharmaceutical composition for preventing or treating hearing loss comprising avocado oil as an active ingredient.

The ears classify the ear canal and the ear canal into the outer ear, the eardrum and ossicles in the middle ear, and the cochlea and the auditory nerve in the inner ear.

Sound is an acoustic energy transmitted through the auricle and outer ear canal to vibrate the eardrum, and the vibration of the eardrum is transmitted to the ossicles by generating mechanical energy. The last bone of the ossicle, the spine, is connected to the cochlea, which transfers the delivered energy to the lymph fluid in the cochlea. The energy delivered to the lymph fluid causes a wave in the lymph fluid, which is stimulated by the hair cells in the cochlea. As the hair cells move, the neurons are transferred to the auditory nerves attached to the hair cells, and the sound energy is transmitted from the auditory nerve to the brain in the form of electrical energy.

As a result, the hearing loss can be improved by the treatment. This hearing loss is called conductive hearing loss. On the other hand, the hearing loss caused by a problem in the brain that plays a comprehensive role, such as the cochlea which is a sound sensing organization, the auditory nerve transmitting sound by electric energy, and the discrimination and understanding of sound is called sensorineural hearing loss.

The auditory organ in the body's sensory organs is one of the most important senses to acquire language and knowledge, social life and human life with the most basic sense of communication. Most of the hearing loss corresponds to sensory neural deafness, which has not been treated so far except for the treatment. Once hearing loss occurs, hearing aids or other mechanical means are used or implanted into the body. Sensory neural deafness can be classified according to the cause and timing of onset. Depending on the timing, it is classified into congenital deafness and acquired hearing loss. Congenital hearing loss is the result of hearing loss caused by damage before birth, caused by genetic problems, problems in the womb, or problems in the birth process.

Congenital hearing loss is mostly severe, and learning a language is impossible without training or education. Most congenital hearing loss implants hearing aids or cochlear implants to hear sounds. However, the severity of hearing loss in such ancillary devices is so low that it is not as good as normal listening ability and daily life is still inconvenient. On the other hand, acquired hearing loss is caused by illness, noise, medication or accident after birth, and the causes are noise, drugs, aging, trauma, and viruses. Recently, hearing loss due to noise and aging is increasing rapidly.

With the development of science and technology, the life expectancy of man has been lengthened, and the elderly population is rapidly increasing worldwide. Most of senile hearing loss is sensory neural deafness, and there are no medications or treatment methods for treatment or improvement besides prevention and management.

In addition, due to the industrialization of society, the hearing loss due to noise is also rapidly increasing. In addition to occupational noise-induced hearing loss such as workers and soldiers working in a noisy environment, noise and hearing loss are also increasing due to cultural and leisure activities. In this regard, Occupational Safety and Health Administration (OSHA) in the United States is implementing noise management for noise environments above 85 dBA. Studies have shown that human auditory organs are affected by noise above 75 dBA, since the noise level of 75 dBA is the noise level of the roadside, so that in modern society, everyone lives in a level of noise that is harmful to the hearing organ can see. In addition, many people are exposed to loud sounds in leisure activities such as MP3 use, and noise-induced hearing loss occurs at various ages.

In the young age, noise-induced hearing loss eventually hurts hearing ability to use hearing aids from the forties, and when physical aging is accompanied by this, the degree of hearing loss is getting worse. As the degree of hearing loss becomes worse, the effectiveness of assistive devices decreases, so serious hearing damage causes serious communication problems.

In this respect, Korean Patent Application No. 10-2008-0044810 discloses that the golden extract has a significant effect of preventing hearing damage in an animal model of noise-null hearing loss. In addition, antioxidants, NMDA (N-methyle-D-aspartate) antagonists, cell death inhibitors, growth factors and the like have been studied as effective substances for preventing or treating hearing loss. However, such substances have been limited to clinical trials.

Thus, the inventors have found that, while developing a substance that can be used for the treatment of hearing loss, the oil of avocado, especially the oil milk from avocado shell, significantly increases the number of hair cells reduced by kanamycin in the zebrafish model By confirming, the present invention has been completed.

Korean Patent Application No. 10-2008-0044810

It is an object of the present invention to provide a composition for the treatment of hearing loss comprising avocado oil and a method of producing the same.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating hearing loss comprising avocado oil as an active ingredient.

The present invention also provides a health functional food for improving hearing loss comprising avocado oil as an active ingredient.

The present invention also provides a method for producing avocado oil comprising milking avocado at a temperature of 40 to 70 캜.

The avocado oil according to the present invention, particularly the avocado shell oil milked from the shell of avocado, significantly increases the number of hair cells reduced by kanamycin in the zebrafish model, so that the avocado oil can be useful for the treatment of deafness have.

FIG. 1 is a graph showing that at the cellular level, the number of hair cells reduced by the oil obtained from the flesh, hulls or seeds of avocado is reduced by kanamycin.
FIG. 2 is a graph showing the concentration of avocado peel oil (AVPO), avocado fructitized butylolyglycolic acid extract (BGAVO), commercially available avocado flesh oil I (AVOI) or avocado flesh oil II (AVOII) at a concentration of 1 or 10 μg / (NOR: untreated control group, KM: negative control group), which was obtained by microscopic observation of the increase of hair cells of zebrafish reduced by kanamycin.
FIG. 3 is a graph showing the results obtained when the avocado peel oil (AVPO), avocado fructus butanol extract (BGAVO), commercially available avocado flesh oil I (AVOI) or avocado flesh oil II (AVOII) (NOR: untreated control group, KM: negative control group) when kanamycin-added zebrafish cells were added.
Fig. 4 shows the results when the avocado oil (AVPO), the ethanol extract of avocado skin (AVPE), the ethanol extract (AVUE) of avocado flesh or the ethanol extract (AVSE) of avocado seed were added at a concentration of 1 or 10 / / (NOR: untreated control group, KM: negative control group), which was obtained by microscopic observation of the increase of hair cells of zebrafish reduced by kanamycin.
Figure 5 shows that when AVPO of avocado, AVPE of avocado bark, AVUE of avocado pulp or AVSE of avocado were added at a concentration of 1 or 10 mu g / mL, (NOR: untreated control group, KM: negative control group), which was decreased by kanamycin.
FIG. 6 shows the results of analysis of components of avocado peel, avocado pulp, or avocado seed oil by HPLC equipped with a 209 nm UV detector.
FIG. 7 shows the results of analysis of components of avocado peel, avocado pulp, or avocado seed oil by HPLC equipped with a 254 nm UV detector.
Figure 8 shows the results of analysis of components of avocado peel, avocado pulp, or avocado seed oil by HPLC equipped with a 280 nm UV detector.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating hearing loss comprising avocado oil as an active ingredient.

The avocado oil may be any one or more selected from the group consisting of the shells, pulp and seeds of avocados, and more specifically milk oiled from the shell of avocado. The avocado oil may be extracted by a method of obtaining an oil of a natural product such as a compression method, a solvent extraction method, a supercritical carbon dioxide extraction method, or a steam distillation method. In one embodiment of the present invention, the avocado oil may be milked by a pressing method.

As used herein, the term " avocado oil " refers to oils extracted from avocado by methods such as compression, leaching and distillation. The avocado oil may be used in the expression such as avocado essence, ointment, essential oil and essential oil.

The hearing loss may be any one or more selected from the group consisting of total hearing loss and sensory nerve impairment. The sensory neuron deafness may be hearing loss caused by noise, drugs, aging, trauma, viruses, and the like, and may be specifically toxic hearing loss. This toxic hearing loss is caused by the toxic drugs gentamicin, streptomycin, kanamycin, neomycin, amikacin, tobramycin, netilmicin, Administration of one or more drugs selected from the group consisting of diclofenac, dibekacin, sisomycin, livodomycin, cisplatin, carboplatin and oxaliplatin Can be caused by hearing loss. Specifically, the toxic hearing loss may be caused by administration of kanamycin.

In one specific embodiment of the present invention we have made oils from the shells, pulp or seeds of avocados, and in particular the avocado shell oil milked from the shell of avocados has been shown to reduce the number of hair cells in zebra fishes reduced by kanamycin (See FIG. 1).

The present inventors also found that when the butylene glycol extract (BGAVO), avocado flesh oil I (AVOI) or avocado flesh oil II (AVOII) of avocado pulp was treated with a zebrafish model reduced by kanamycin, (Fig. 2 and Fig. 3), while the number of hair cells was not significantly changed compared with that of avocado skin oil (AVPO).

The present inventors also found that when the ethanol extract (AVPE) of avocado shell, the ethanol extract (AVUE) of avocado pulp, or the ethanol extract (AVSE) of avocado seed were treated with a zebrafish model reduced by kanamycin, The number of cells was not significantly changed, whereas the number of hair cells was significantly increased during treatment with avocado oil (AVPO) (see FIGS. 4 and 5).

Therefore, the avocado flesh oil of the present invention can be useful for the treatment of deafness.

The pharmaceutical composition according to the present invention may contain 10 to 95% by weight of avocado oil as an active ingredient, based on the total weight of the composition. In addition, the pharmaceutical composition of the present invention may further contain at least one active ingredient which exhibits the same or similar functions in addition to the above-mentioned effective ingredients.

The pharmaceutical compositions of the present invention may include carriers, diluents, excipients or mixtures thereof conventionally used in biological preparations. Pharmaceutically acceptable carriers can be used as long as they are suitable for delivering the composition in vivo. Specifically, the carrier is selected from Merck Index, 13th ed., Merck & Sterile water, Ringer's solution, dextrose solution, maltodextrin solution, glycerol, ethanol, or a mixture thereof. If necessary, conventional additives such as an antioxidant, a buffer, a bacteriostatic agent and the like may be added.

When the composition is formulated, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient may be added.

The compositions of the present invention may be formulated into oral or parenteral formulations. Oral preparations may include solid preparations and liquid preparations. The solid preparation may be a tablet, a pill, a powder, a granule, a capsule, or a troche. The solid preparation may be prepared by adding at least one excipient to the composition. The excipient may be starch, calcium carbonate, sucrose, lactose, gelatin or a mixture thereof. In addition, the solid preparation may comprise a lubricant, examples of which include magnesium stearate, talc, and the like. On the other hand, the liquid preparation may be a suspension, a solution, an emulsion or a syrup. At this time, the liquid preparation may contain an excipient such as a wetting agent, a sweetener, a fragrance, a preservative, and the like.

The parenteral preparation may contain injections, suppositories, respiratory inhalation powders, aerosol formulations for spraying, powders and creams. The injection may include a sterilized aqueous solution, a non-aqueous solvent, a suspending agent, an emulsion, and the like. As the non-aqueous solvent or suspension, vegetable oils such as propylene glycol, polyethylene glycol and olive oil, and injectable esters such as ethyl oleate may be used.

The composition of the present invention can be administered orally or parenterally according to the desired method. Parenteral administration may include intraperitoneal, rectal, subcutaneous, intravenous, intramuscular or intrathecal injection.

The composition may be administered in a pharmaceutically effective amount. This may vary depending on the type of disease, severity, activity of the drug, sensitivity of the patient to the drug, administration time, route of administration, duration of treatment, However, for the desired effect, the amount of the active ingredient contained in the pharmaceutical composition according to the present invention may be 0.0001 to 10,000 mg / kg, specifically 0.001 to 5 g / kg. The administration may be several times per day.

The composition of the present invention may be administered alone or in combination with other therapeutic agents. When administered concomitantly, the administration can be sequential or simultaneous.

The present invention also provides a health functional food for improving hearing loss comprising avocado oil as an active ingredient.

The avocado oil according to the present invention may have the characteristics as described above. In one example, the avocado oil may be an oil that is milked from at least one selected from the group consisting of shells, pulp and seeds of avocados, and in particular may be an oil that is milked from the shell of avocado.

The above-described hearing loss is as described above. For example, the hearing loss may be any one or more selected from the group consisting of pre-speech hearing loss and sensory nerve impairment, and specifically may be sensory nerve impairment. The sensory neural deafness may be this toxic hearing loss.

In one specific embodiment of the present invention we have made oils from the shells, pulp or seeds of avocados, and in particular the avocado shell oil milked from the shell of avocados has been shown to reduce the number of hair cells in zebra fishes reduced by kanamycin (See FIG. 1).

The present inventors also found that when zebrafish reduced by kanamycin was treated with FG (BGAVO), avocado flesh oil I (AVOI) or avocado flesh oil II (AVOII) of avocado, (Fig. 2 and Fig. 3), while the number of hair cells was not significantly changed compared with that of avocado skin oil (AVPO).

The present inventors also found that when the ethanol extract (AVPE) of avocado shell, the ethanol extract (AVUE) of avocado pulp, or the ethanol extract (AVSE) of avocado seed were treated with a zebrafish model reduced by kanamycin, The number of hair cells was significantly increased as compared to the ethanol extract treatment of avocado shell oil (AVPO) treatment (see FIGS. 4 and 5), while there was no significant change in the number of cells.

Therefore, the avocado flesh oil of the present invention can be usefully used for improving the hearing loss.

The composition of the present invention can be added directly to food or used together with other food or food ingredients. At this time, the content of the active ingredient to be added may be determined depending on the purpose, and may generally be 0.01 to 90 parts by weight of the total food weight.

The form and kind of the health functional food are not particularly limited. Specifically, the health functional food may be in the form of a tablet, a capsule, a powder, a granule, a liquid and a ring. The health functional food may contain various flavors, sweeteners or natural carbohydrates as an additional ingredient. The sweeteners may be natural or synthetic sweeteners, examples of natural sweeteners include tau martin and stevia extract. On the other hand, examples of synthetic sweeteners include saccharin and aspartame. The natural carbohydrates may be monosaccharides, disaccharides, polysaccharides, oligosaccharides, sugar alcohols, and the like.

The health functional food of the present invention may contain, in addition to the above-described additional ingredients, a nutrient, a vitamin, an electrolyte, a flavoring agent, a colorant, a pectin and a salt thereof, an alginic acid and its salt, an organic acid, a protective colloid thickening agent, , Glycerin, alcohol, and the like. These components may be used independently or in combination. The proportion of the additive may be selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a process for producing avocado oil.

The avocado oil may be an oil that is milked from the shell of avocado. The avocado oil may be extracted by a method of obtaining an oil of a natural product such as a compression method, a solvent extraction method, a supercritical carbon dioxide extraction method, or a steam distillation method. In one embodiment of the present invention, the avocado oil may be milked by a pressing method.

The manufacturing method may include milking avocados.

The temperature for milking the avocado may be 40 to 70 캜, specifically 45 to 65 캜, more specifically 50 to 60 캜, and still more specifically 52 to 63 캜. Milking is not sufficiently carried out within a range beyond the temperature condition for milking the avocado, so that the content of the active ingredient may be insufficient. Or the content of components exhibiting toxicity may increase.

In addition, in the above production method, the step of centrifuging the milked avocado oil to obtain a supernatant may be further included.

The temperature condition for centrifuging the above milked avocado oil may be 10 to 40 캜, specifically 10 to 35 캜, more specifically 12 to 30 캜, still more specifically 15 to 25 캜. The rotation conditions for centrifuging the milked avocado oil may be 8,000 to 16,000 rpm, specifically 9,000 to 15,000 rpm, more specifically 10,000 to 14,000 rpm, even more specifically 11,000 to 13,000 rpm. Centrifugation may not be performed sufficiently or excessively in a range beyond the condition of centrifuging the above milked avocado oil, so that the content of the active ingredient in the supernatant liquid may be insignificant or the content of the non-active ingredient may be high.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, but the present invention is not limited thereto.

Example 1. Preparation of avocado shell oil

The avocado (from the United States) was peeled to give 612.1 g of a shell. The obtained shell was dried at a temperature of 55 DEG C for 48 hours to obtain 138.5 g of dried avocado shell. From the dried avocado shells, avocado shell oil was prepared at a temperature of 55 ° C using an Xpella-type milking machine (OilLube Premium LENCHI AUTO, NATIONAL ENG CO., LTD.). The prepared avocado shell oil was centrifuged at 20 DEG C and 12,000 rpm to obtain only supernatant, which was then filtered to obtain 70.8 g of avocado shell oil to be used for the experiment at a yield of 11.56%. The avocado shell oil was stored at room temperature until use.

Comparative Example  1. Preparation of avocado pulp oil

Avocado flesh oil was prepared under the same conditions and procedures as in Example 1 except that 986.8 g of avocado flesh was used. As a result, 175.7 g of avocado flesh oil was obtained in a yield of 17.8%.

Comparative Example  2. Production of avocado seed oil

Avocado seed oil was prepared under the same conditions and procedures as in Example 1, except that 207.5 g of avocado seed was used. As a result, 4.7 g of avocado seed oil was obtained in a yield of 2.3%.

Comparative Example  3. Avocado butylene Glycoll  Preparation of extract

1.063 g of avocado (US) flesh was dried at a temperature of 55 ° C for 48 hours to yield 302 g of dried avocado flesh. 500 ml of butylene glycol was added to the dried avocado flesh and the butylene glycolysis extract of avocado flesh was prepared using an ultrasonic extractor at 55 ° C for 1 hour. The obtained extract was filtered and concentrated to obtain 79.2 g of butylene glycolysis extract of avocado pulp with a yield of 7.45%.

Comparative Example  4. Preparation of avocado ethanol extract

<4-1> Ethanol extract of avocado skin

2400 g of avocado (US) shell was dried at a temperature of 55 ° C for 48 hours to obtain 500 g of dried avocado shell. 1000 ml of 70% ethanol was added to the dried avocado shell and ultrasonically extracted at 55 ° C for 1 hour at 40 kHz to prepare an ethanol extract of avocado shell. The obtained extract was filtered and concentrated to obtain 48.25 g of an ethanol extract of avocado skin in a yield of 9.65%.

<4-2> Ethanol extract of avocado pulp

2000 g of avocado (US) flesh was dried at a temperature of 55 ° C for 48 hours to yield 500 g of dried avocado flesh. 1000 ml of 70% ethanol was added to the dried avocado flesh, and the ethanol extract of avocado flesh was prepared by sonication at 55 k &lt; 0 &gt; C for 1 hour at 40 kHz. The obtained extract was filtered and concentrated to obtain an ethanol extract of 71 g of avocado pulp with a yield of 14.2%.

<4-3> Ethanol extract of avocado seed

3000 g of avocado (US) seeds were dried at a temperature of 55 ° C for 48 hours, yielding 500 g of dried avocado seed. 1000 ml of 70% ethanol was added to the dried avocado seed, and the resulting extract was sonicated at 55 k &lt; 0 &gt; C for 1 hour at 40 kHz to prepare an ethanol extract of avocado seed. The obtained extract was filtered and concentrated to obtain 62.5 g of an ethanol extract of avocado seed in a yield of 12.5%.

Experimental Example  One. Zebra fish  Comparison of the effect of avocado bark, seeds and flesh oil on the innards toxicity in the model

The effects of avocado shells, pulp and seed oil prepared by Example 1 and Comparative Examples 1 and 2 on hair cells reduced by kanamycin were confirmed by the following method.

Specifically, 6 days after fertilization, zebrafish larvae were placed in a 24-well plate, and 25 mu M kanamycin was added. After 30 minutes, kanamycin was removed and the shells, flesh and seed oil of the avocado prepared in Examples 1 to 3 were added at a concentration of 1, 10, 50, 100, 250 or 500 占 퐂 / ml. At this time, a 0.03% (w / v) sea salt solution was used as a control. After 12 hours, 0.1% YO-PRO (Life Technologies, USA) was added to stain the hair cells of the fry for 30 minutes. Dyed hair cells were observed using a fluorescence microscope (Olympus 1x70, Olympus, Japan), and the number of hair cells observed was counted. As a result, the number of hair cells observed by fluorescence microscopy is shown in Fig.

As shown in Fig. 1, avocado skin oil significantly increased hair cells of zebra fish fry compared to pulp and seed oil (Fig. 1).

Experimental Example  2. Zebra fish  Comparison of effects of poison ivy on avocado peel oil and avocado flesh oil and avocado fructose butylanalcohol extract on model

AVOI, AGROCOMERCIAL CAMPESINA, Mexico) and avocado flesh oil II (AVOII, AVOCADO OIL NEW ZEALAND, manufactured by Nisshinbo Industries, Inc.) and the commercially available avocado flesh oil I (AVOI, AGROCOMERCIAL CAMPESINA, Mexico) LTD, New Zealand) on the hair cells reduced by kanamycin were compared in the following manner.

Specifically, the zebrafish fry after 6 days of fertilization was placed in a 24-well plate and kanamycin at a concentration of 25 μM was added. After 30 minutes, the kanamycin was removed, and 1, or 10 / / ml Avocado Peel Oil (AVPO), the Butyleneglycolic Acid Extract (BGAVO) of Avocado Flesh, the Commercial Avocado Fruit Oil I (AVOI) II &lt; / RTI &gt; (AVOII). At this time, 0.03% (w / v) sea salt solution was used as a control. After 12 hours, 0.1% YO-PRO was added to stain the hair cells of the fry for 30 minutes. Dyed hair cells were observed using a fluorescence microscope (Olympus 1x70, Olympus, Japan), and the number of hair cells observed was counted. As a result, photographs of the hair cells observed by fluorescence microscopy are shown in Fig. 2, and the number of hair cells measured is shown in Fig.

As shown in Figs. 2 and 3, the number of hair cells was significantly increased in avocado skin oil-supplemented fry compared to the negative control (KM) treated with kanamycin, and the amount of butylolyglycolic extract (BGAVO) The number of hairy cells was significantly increased in avocado shell oil-supplemented fry compared to the experimental group supplemented with commercially available two kinds of avocado flesh oil (AVOI, AVOII) (FIGS. 2 and 3).

Experimental Example  3. Zebra fish  Comparison of effects of poisoning of avocado peel oil and ethanol extracts of avocado bark, flesh and seed in the model

The effects of the ethanol extracts of avocado shell oil, avocado shell, pulp and seed prepared in Example 1 and Comparative Example 4 on the hair cells reduced by kanamycin were compared in the following manner.

Specifically, the ethanol extract (AVPE) of avocado skin (AVPO), the avocado skin extract (AVPE), the ethanol extract of avocado flesh (AVUE), or the ethanol extract of avocado seed prepared in Example 1 and Comparative Example 4 (AVSE) were added to the hair cells, the hair cells were analyzed under the same conditions and in the same manner as in Experimental Example 1. As a result, photographs of the hair cells observed by fluorescence microscopy are shown in Fig. 4, and the number of hair cells measured is shown in Fig.

As shown in FIGS. 4 and 5, the number of hair cells was significantly increased in avocado skin oil supplemented fish compared to the negative control (KM) treated with kanamycin, and the ethanol extracts of avocado skin, pulp, The number of hair follicle cells increased in fry to which avocado skin oil was added compared to the experimental group added (Figs. 4 and 5).

Experimental Example  4. Analysis of components of avocado skin, pulp and seed oil

To compare the components of the avocado shell, pulp and seed oil prepared from Example 1 and Comparative Examples 1 and 2.

Specifically, samples of avocado shell, pulp and seed oil prepared in Example 1 and Comparative Examples 1 and 2 were analyzed by HPLC (High performance liquid chromatography) equipped with a C18 column. The column used was a C18 column (Ischoff Chromatography, ProntoSIL C 18 column (250 * 4.5 mm, 5 탆)) and a detector using 209, 254, and 280 nm UV detectors. As the mobile phase, 0.2% formic acid and acetonitrile were used in proportions adjusted according to the analysis time as shown in Table 1. The run time was 55 minutes, the sample injection amount was 10 μl, The temperature of the column was set at 35 캜. As a result, HPLC results of avocado shell, pulp and seed oil measured at 209, 254 and 280 nm are shown in Figs.

minute 0.2% formic acid Acetonitrile 0 85 15 35 35 65 40 0 100 42 0 100 55 85 15

As shown in Figs. 6 to 8, the components of the avocado shell oil prepared in Example 1 were different from those of the avocado pulp and seed oil prepared in Comparative Examples 1 and 2, The content was remarkably high.

Claims (10)

A pharmaceutical composition for preventing or treating hearing loss comprising avocado oil as an active ingredient.
The pharmaceutical composition according to claim 1, wherein the avocado is at least one selected from the group consisting of the skin, flesh and seed of avocado.
The pharmaceutical composition according to claim 2, wherein the avocado is a bark of avocado.
The pharmaceutical composition according to claim 1, wherein the hearing loss is at least one selected from the group consisting of hearing loss and hearing loss.
The pharmaceutical composition according to claim 1, wherein the avocado oil is prepared by a compression method, a solvent extraction method, a supercritical carbon dioxide extraction method, or a steam distillation method.
The pharmaceutical composition according to claim 1, wherein the avocado oil is oil, avocado essence, perfume oil, essential oil or essential oil.
A health functional food for improving hearing loss containing avocado oil as an active ingredient.
The health functional food according to claim 7, wherein the avocado is at least one selected from the group consisting of shells, pulp and seeds of avocado.
9. The health functional food according to claim 8, wherein the avocado is the skin of avocado.
8. The health functional food for improving the hearing loss according to claim 7, wherein the hearing loss is at least one selected from the group consisting of former hearing loss and sensory nerve impairment.

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