KR20180134232A - Cosmetic composition comprising wheat-chaff extract - Google Patents

Abstract

Provided is a cosmetic composition containing a wheat bark extract as an active ingredient and having a wrinkle-ameliorating and/or skin-whitening function. The inventors of the present invention completed the present invention upon confirming outstanding functionality of wheat bark in terms of collagen synthesis, antioxidation, and whitening effects when the wheat bark conventionally classified as waste materials is extracted and used as the cosmetic composition.

Description

[0001] Cosmetic composition comprising wheat-chaff extract [

The present invention relates to a cosmetic composition comprising an extract of wheat husks and a process for producing the same.

Although interest in natural products using plants has been increasing day by day due to well-being consumption trend, there is no development and utilization of natural functional materials using food crops using by-products except cereals. In Korea and abroad, some researches have been carried out to harvest crops and utilize the remaining byproducts as feeds, but in Korea, they are limited to research using rice byproducts.

Although some studies on the value of wheat straw as a feed have been reported, research on the use of biofuels as a biofuel crop is under way, And economic problems. There are also a number of other commercially available methods, such as the conversion to polyurethane used in synthetic fibers and paints (Latvia) or the conversion to lactic acid, which is a raw material for paper (Denmark) There is research and development used as dishware cleaner. However, there is almost no research on the development and utilization of the byproducts obtained after harvesting wheat as a functional raw material for food or cosmetics.

Conventionally developed anti-aging components are mainly mimic peptides of growth factors such as TGF-β. However, in the elderly, the main target of anti-aging ingredients, the activity of MMP is abnormally high and the function of collagen is not properly responded to the mechanism of promoting collagen synthesis. Therefore, the substance that promotes collagen production is applied to the elderly There is a limit to doing.

MMP inhibitors have been studied not only for cosmetic materials but also for the development of pharmaceuticals. However, the development of MMP inhibitors with special effects has not been achieved so far. In the case of cosmetic materials, Pepha-TIMP from Pentapharm is used as such a concept, but it is a recombinant TIMP-2 (21.7 kDa), which is an inhibitor of MMP-2 in the human body. It is not infiltrated, it is expensive, and its activity is not excellent, so there are many problems in use.

Retinoic acid, which is used as a drug ingredient, has excellent MMP-1 inhibitory ability, which has already been reported through various publications, and has a powerful MMP-1 inhibiting ability as well as an antioxidant effect, It is medicine. Since retinoic acid, which is a drug for cosmetics, can not be used, its derivative, retinol or other types of retinoids are used. However, the MMP-1 inhibitory ability of these derivatives is almost insignificant compared to retinoic acid. ) In particular, because of the instability and irritation of retinoids, the use of night creams that do not receive ultraviolet rays is limited.

Therefore, it is necessary to develop a material which is stronger than MMP inhibitory substances and has stimulating properties for collagen synthesis, which has antioxidant and complex functions, but which is not irritating to the human body, as a new concept material than retinoid.

An object of the present invention is to provide a cosmetic composition comprising wheat bark extract having a function of promoting collagen biosynthesis, antioxidation and whitening effect.

It is another object of the present invention to provide a method for producing a cosmetic composition comprising wheat bark extract.

The inventors of the present invention completed the present invention upon confirming that wheat husks classified as waste resources are extracted and used as a cosmetic composition in terms of functionality such as collagen synthesis, antioxidation and whitening effect.

The present invention relates to a cosmetic composition or composition comprising wheat bark extract as an active ingredient, and is excellent in collagen biosynthesis promotion, antioxidative activity and whitening activity.

The cosmetic composition of the present invention may be a cosmetic composition for accelerating collagen biosynthesis, anti-aging, antioxidant or whitening. The cosmetic composition of the present invention is excellent in promoting collagen biosynthesis, anti-aging, antioxidant properties or whitening activity, and is thus easily applicable to functional cosmetics.

The cosmetic composition according to the present invention contains a by-product of wheat, that is, a wheat bark extract, as an active ingredient. Since the wheat can grow in a clean environment, it is a pollution-free clean plant. Therefore, It can be used as a supply source.

Hereinafter, the present invention will be described in more detail.

An example of the present invention provides a cosmetic composition comprising wheat bark extract as an active ingredient. The cosmetic composition of the present invention may be a cosmetic composition for accelerating collagen biosynthesis, anti-aging, antioxidant or whitening. The cosmetic composition of the present invention is excellent in promoting collagen biosynthesis, anti-aging, antioxidant properties or whitening activity, and is thus easily applicable to functional cosmetics.

The cosmetic composition of the present invention has an activity of promoting collagen biosynthesis, inhibiting collagen decomposition, and thus improving wrinkles of the skin, and can be used for these purposes. Specifically, the cosmetic composition of the present invention is excellent in inhibitory activity against collagenase or MMP (matrix metalloproteinase), and inhibits the activity of MMP, thereby improving wrinkles or preventing skin aging.

Collagen proteins in the dermal extracellular tissues impart strength and tension to the skin, which protects the skin from external stimuli or forces. It accounts for 90% of the dermal layer. Collagen reduction is closely related to skin aging and wrinkle formation . Decomposition of collagen degrades as aging progresses. When exposed to stimuli such as ultraviolet rays, biosynthesis of collagenase increases, collagen synthesis is reduced and wrinkles are formed. Collagenase is a zinc-dependent intracellular protease that breaks down or restructures the extracellular components of the dermis. MMP-1 acts on the collagen of the dermis and breaks it down. Therefore, the regulation of the amount and activity of MMP enzyme synthesis plays an important role in wrinkles, and the cosmetic composition inhibits MMP activity, thereby improving wrinkles or preventing skin aging.

The cosmetic composition of the present invention is excellent in antioxidant activity and can be used for antioxidant use. Specifically, as a result of measuring changes in intracellular reactive oxygen concentration using DCFDA by treating the wheat bark extract, the antioxidative effect was similar to or superior to that of glutathione, which is conventionally known as a strong antioxidant. In particular, Shell extracts.

For example, the relative oxidation rate (%) of the wheat bark extract at 10 μg / ml is 30 to 135%, preferably 50 to 120% based on 100% of the oxidation rate of 10 mM glutathione calculated by the following formula 1, 60 to 120% or 85 to 120%.

For example, the relative oxidation rate can be calculated by the following equation (2).

The cosmetic composition of the present invention is excellent in whitening activity and can be used for whitening purposes. Specifically, the wheat bark extract-treated melanoma cells were measured for their absorbance and the whitening effect was confirmed. As a result, it was confirmed that the whitening effect was excellent. For example, the melanoma cell may have an activity of inhibiting melanin biosynthesis, and specifically may have a tyrosinase inhibiting activity.

Wheat is an annual herbaceous plant of the unicorn plant. It can be cultivated without the use of herbicides and other pesticides due to the cultivation characteristics of sowing in autumn and harvesting in early summer (early June).

The cosmetic composition according to the present invention contains a by-product of wheat, that is, a wheat bark extract, as an active ingredient. Since the wheat can grow in a clean environment, it is a pollution-free clean plant. Therefore, It can be used as a supply source.

Spikelets run directly on the spikes' rachis, and mature seeds are usually enclosed by the outer shell (lemma) and the palea (chaff). The wheat husks of the present invention may be at least one selected from the group consisting of crusts and crusts, preferably crusts. The crust is easily separated when it is thawed after harvesting and can be collected so as not to be contaminated, which is advantageous in the development of natural functional materials utilizing this.

Specifically, extracts of wheat seeds with wheat husks removed have an effect of lowering free radicals regardless of cultivars, and this effect is remarkably superior to that of wheat husk seed extracts.

The wheat applicable to the present invention may be at least one kind selected from the group consisting of the raw materials, landscaping, olive, gold, and we, but the variety is not particularly limited.

As used herein, wheat bark extracts may be in the form of solutions, concentrates or powders, including crude extracts of wheat bark or certain solvent-soluble extracts or fractions of wheat bark. In one embodiment of the present invention, the wheat extract is a crude extract obtained by extracting a wheat husk with at least one solvent selected from the group consisting of water, linear or branched alcohols having 1 to 4 carbon atoms, an organic solvent and a mixed solvent thereof, Soluble extract obtained by adding at least one solvent selected from the group consisting of water, a linear or branched alcohol having 1 to 4 carbon atoms, an organic solvent and a mixed solvent thereof to the crude extract. The organic solvent may be at least one selected from the group consisting of dichloromethane, diethylether, chloroform and ethyl acetate, preferably dichloromethane and / or ethyl acetate, but is not limited thereto.

The wheat bark extract may be prepared using conventional extraction methods, such as, but not limited to, cold extraction, hot water extraction, ultrasonic extraction, reflux extraction or cooling extraction, preferably reflux cooling extraction. The extraction step may be repeated one or more times, and the extraction efficiency may be enhanced by mixing and extracting the extract extracted with the second or higher order and the filtrate obtained after each extraction.

The extract obtained in the extraction step is suspended in water having a volume of about 2 to 10 times, preferably about 3 to 7 times, and then water, a linear or branched alcohol having 1 to 4 carbon atoms, an organic solvent and a mixed solvent thereof A method for preparing a solvent-soluble extract by extracting a solvent-soluble layer by adding at least one solvent selected from the group consisting of water, ethanol, and water, and concentrating under reduced pressure, thereby purifying impurities such as unnecessary proteins, polysaccharides and fatty acids.

And then filtering or concentrating the extract. The concentrate produced by the concentration step may be used in an amount of from about 10 to 30 times, preferably from 15 to 25 times, more preferably from about 10 times to about 30 times the total amount of the concentrate for controlling the content of the remaining solvent so as to be suitable for use as a cosmetic or pharmaceutical raw material By concentrating the mixture in an amount of about 20 times by weight with water in an amount of 1 to 5 times, preferably 2 to 3 times, adding the same amount of water again and homogenously suspending it, and then sterilizing and / or lyophilizing it to prepare a powdery wheat bark extract .

In the cosmetic composition of the present invention, the wheat bark extract may be contained in an amount of 0.0001 to 80% by weight, preferably 0.001 to 30% by weight, based on the solid content of the whole composition.

The cosmetic composition of the present invention may contain, in addition to the active ingredient, all kinds of components which can be used for ordinary commercialization or formulation such as surfactant, emulsifier, soap acid, solvent, binder, diluent, lubricant, stabilizer, A preservative, an antioxidant, a defoaming agent, an antimicrobial agent, an enzyme, a plant or mineral oil, a fat, a fluorescent substance, a fungicide, a hygroscopic substance, a humectant, a fragrance, a fragrance carrier, The composition may contain one or more pharmacologically acceptable excipients selected from the group consisting of protein, silicone, solubilizing agent, saccharide derivative, sunscreen agent, vitamin, plant extract and wax, And can be appropriately adjusted depending on the application and purpose of use.

In addition, the cosmetic composition may contain a solvent ordinarily included in the application form, and examples thereof include ethanol, glycerin, butylene glycol, propylene glycol, polyethylene glycol, 1,2,4-butanetriol, sorbitol ester , 1,2,6-hexanetriol, benzyl alcohol, isopropanol, butanediol, diethylene glycol monoethyl ether, dimethylisosorbide, N-methyl-2-pyrrolidone, propylene carbonate, glycereth- At least one member selected from the group consisting of isosetyl myristate, isocetyl octanoate, octyldodecyl myristate, octyldodecanol, isostearyl diacetate, cetyl octanoate, neopentyl glycol dicaprate, etc. . ≪ / RTI > Those skilled in the art will appreciate that the type and amount of solvent may be suitably selected according to the characteristics of the product.

In addition, the cosmetic composition may contain various substances for enhancing transdermal permeation during transdermal administration. For example, there may be mentioned, for example, laurolactam derivatives and oleic acid, ester derivatives of monooleate derivatives, aderapalene, tritinoin, retinaldehyde, tazotrotin, salicylic acid, azelaic acid, glycolic acid, ethoxydiglycol, 80, lecithin olganogel, and the like. In order to impart additional functions, the cosmetic composition of the present invention may contain a co-surfactant, a surfactant, an anti-dandruff agent, a keratin softener, a blood circulation accelerator, a cell growth inhibitor, An auxiliary agent such as an active agent, a refreshing agent, a moisturizing agent, an antioxidant, a pH adjusting agent and purified water may be added. Depending on the application form, it may contain additives such as suitable fragrance, pigment, preservative and excipient.

The composition of the present invention may be a cosmetic composition for improving wrinkles.

For example, there may be promoted collagen biosynthesis or collagen degradation inhibitory activity, and more specifically, promoting collagen biosynthesis by increasing the expression of procollagen, beta actin and / or colla1 gene, inhibiting collagen degradation by inhibiting MMP synthesis .

The wrinkle improving application may be by improving the antioxidant effect. For example, the relative oxidation rate (%) of the wheat bark extract may be 30 to 135% based on 100% of the oxidation rate of glutathione calculated by Equation 1 below, Preferably 50 to 120%, more preferably 60 to 120%.

[Equation 1]

For example, the relative oxidation rate can be calculated by the following equation (2).

&Quot; (2) "

As another example of the present invention, there is provided a collagen biosynthesis promoter or collagenase inhibitor comprising wheat bark extract as an active ingredient.

For example, the collagen biosynthesis promoter may be an expression promoter of procollagen, beta actin and / or colla1, and the collagenase inhibitor may be, for example, a matrix metalloproteinase (MMP), preferably an MMP-3 inhibitor .

As another example of the present invention, the composition of the present invention provides a skin whitening cosmetic composition comprising wheat bark extract as an active ingredient.

As another example of the present invention, the composition of the present invention provides a melanin biosynthesis inhibitor or tyrosinase inhibitor of Melanoma cells comprising wheat bark extract as an active ingredient.

As another example of the present invention, there is provided a method for producing a cosmetic composition comprising wheat bark extract as an active ingredient.

The matters relating to the above-mentioned cosmetic composition can be similarly applied to the manufacturing method thereof.

For example, the method comprises separating wheat husks from wheat; And extracting the wheat husks with at least one solvent selected from the group consisting of water, linear or branched alcohols having 1 to 4 carbon atoms, organic solvents, and mixed solvents thereof.

Separating the wheat husk from the wheat means removing the wheat seeds from the harvested wheat and separating only the wheat husk and can be treated further, including washing, peeling or filtering the wheat husk It can be finely chopped or powdered, or it can be used after freeze-drying the powder.

In the step of extracting the wheat husk with a solvent, any conventional extraction method may be used. For example, it may be a cold extraction, a hot water extraction, an ultrasonic extraction, a reflux extraction or a cooling extraction, It is not. The extraction step may be repeated one or more times, and the extraction efficiency may be enhanced by mixing and extracting the extract extracted with the second or higher order and the filtrate obtained after each extraction.

The extract obtained in the extraction step is suspended in water having a volume of about 2 to 10 times, preferably about 3 to 7 times, and then water, a linear or branched alcohol having 1 to 4 carbon atoms, an organic solvent and a mixed solvent thereof A method for preparing a solvent-soluble extract by extracting a solvent-soluble layer by adding at least one solvent selected from the group consisting of water, ethanol, and water, and concentrating under reduced pressure, thereby purifying impurities such as unnecessary proteins, polysaccharides and fatty acids.

The step of filtering or concentrating the extract may further comprise the step of: The concentrate produced by the concentration step may be used in an amount of about 10 to 30 times, preferably 15 to 25 times, more preferably about 20 times, By concentrating the mixture with water in a weight ratio of from 1 to 5 times, preferably from 2 to 3 times, adding the same amount of water again and homogenously suspending the mixture, and then sterilizing and / or lyophilizing the mixture to obtain a powdery wheat bark extract.

The present invention is a cosmetic composition comprising wheat bark extract as an active ingredient, and is useful as functional cosmetics because of its excellent collagen biosynthesis promotion, antioxidative activity and / or whitening activity.

FIG. 1A shows the cytotoxicity test of wheat bark extract in HS68 cells.
FIG. 1B shows the cytotoxicity test of wheat bark extract in Hacat cells.
FIG. 2 shows the results of the synthesis of procollagen 1 according to the wheat bark extract treatment of each cultivar, corrected with the amount of beta actin.
FIG. 3 shows the antioxidative effects of wheat bark extract according to the type and concentration.
Fig. 4 shows the whitening effect of wheat bark extract according to the cultivars.
FIG. 5A is a result of measurement of cell oxidation rate according to the wheat bark extract treatment of each kind.
FIG. 5b shows the result of measurement of cell oxidation rate according to the wheat seed extract treatment by breed.
FIG. 6 shows the result of measurement of wheat bark extract-treated samples and their absorbance of the melanoma cells of the rats.

The present invention will be described in more detail with reference to the following examples. However, the scope of protection of the present invention is not intended to be limited to the following examples.

Example  1. Preparation of wheat bark extract

10 g, 20 g, and 40 g of each of the raw materials, landscaping, algae, and gold husks were added to 1 L of an 80% ethanol water mixed solvent, followed by reflux cooling extraction. After 3 hours of extraction, the mixture was filtered under reduced pressure, and the solvent was removed using a rotary vacuum concentrator.

Example 2. Wheat  Seeds and Green body  Extract preparation

10 g, 20 g, and 40 g of the seeds of each of the raw materials, landscape, algae, and gold were added to 1 g of 80% ethanol water mixed solvent in 10 g and 20 g of the green body of the steel and subjected to reflux cooling. After 3 hours of extraction, the mixture was filtered under reduced pressure, and the solvent was removed using a rotary vacuum concentrator.

Example  3. Safety evaluation

To evaluate the stability of the wheat bark extract prepared in Example 1, a cytotoxicity experiment was conducted. Cells were seeded in 96-well plates at a concentration of 1.5 x 104 cells / well and cultured at 37 ° C and 5% CO 2 at 24 ° C for 24 h. Time. 200 μl of the DMEM medium containing 10% FBS diluted with the sample was replaced with the culture medium, followed by further incubation for 24 hours. 20 μl of MTT kit (CellTiter 96, Promega) was added to each well. After 3 hours, the absorbance was measured at 490 nm using a microplate reader (Bioteck), and the cell viability (%).

[Equation 1]

Cell survival rate (%) = S _S / S _C 100

(S _S : absorbance of the group treated with the sample, S _C : absorbance of the group not treated with the sample)

The results are shown in FIGS. 1A and 1B. As a result of MTT analysis, the cell survival rate was not reduced to 20 ppm in all the samples. Therefore, the wheat bark extract was found to be a safe raw material within the effective concentration.

Example  4. Procollagen  Evaluation of synthesis effect

4.1 Analysis of the amount of procollagen and beta actin expression

The human skin fibroblast HS58 was dispensed into 6-well plates at a concentration of 1 * 10 ⁶ cells / well, and cultured at 37 ° C and 5% CO ₂ until the number of cells increased to 70-80% Time. After washing the cells with PBS, UV-A and UV-B were irradiated for 60 seconds with a UV irradiator (BoTeck, Korea). The 20 μg / ml sample prepared in Example 1 is replaced with the culture medium for the serum-free medium, followed by further incubation for 24 hours. After removing the medium, the cells were washed with PBS and 100 μl of SDS sample buffer (120 mM Tris-HCl (pH 6.8), 20% Glycerol, 4% SDS, 28.8 mM 2-mercaptoethanol, 0.01% bromophenol blue) Collected and boiled at a temperature of 105 DEG C for 10 minutes to denature the protein. 20 μl of the denatured sample was subjected to 10% SDS-PAGE electrophoresis, the protein was transferred to a nitrocellulose membrane and then cultured in a 5% skim milk dissolved in TBST buffer for 30 minutes. Then, (Santa Cruze Biotechnology, CA) and Procollagen 1 (Santa Cruze Biotechnology, CA) antibody were diluted 1: 500 in 5% skim milk TBST medium and shaken overnight at 4 ° C. After washing three times for 10 min with TBST, the HRP-conjugated secondary antibody was incubated in a 1: 2000 dilution of 5% skim milk TBST buffer for 2 h. The membranes were washed three times for 10 minutes each with TBST, and incubated in a dark room for 3 minutes with a Novex ECL Chemiluminescent Substrate Reagent Kit reagent (Invitrogen, USA).

FIG. 2 shows a graph in which the amount of procollagen 1 was corrected to the amount of beta actin and the result of development of beta actin and procollagen 1 was analyzed by image J. FIG. It was confirmed that procollagen 1 was synthesized significantly in wheat gum extract of all varieties, and promoted the production of procollagen biosynthesis. Especially, it was confirmed that promoting effect of procollagen biosynthesis is better in landscaping, olive and kumquat cultivars.

4.2 Analysis of expression of COL1A1

The expression level of the col1a1 gene, which is known as the collagen gene, was measured.

Specifically, 72 hours under the conditions of the human skin fibroblast in Hs68 (human skin fibroblast) to 2.2 x 10 after the frequency division to ⁶ cells concentration of 100 pi cell culture dish in 70 ~ 37 ℃ until grow approximately 80%, 5% CO2 Lt; / RTI > Each dish was washed with 10 ml of phosphate buffer saline (PBS), and the cells were washed with UV-A and UV-B for 60 seconds with a UV-irradiator (BoTeck, Korea). Subsequently, the serum-free medium diluted with the sample was replaced with the culture medium and further cultured for 24 hours.

After 24 hours, the medium was removed and the RNA was isolated using hybrid-RTM (GeneAll Biotechnology, Korea), an RNA extraction kit, and cDNA was synthesized using iScript ™ cDNA Synthesis Kit (BIO-RAD, USA). 1 μL (0.5 μg / μL) of each of Col1a1 and GAPDH primers and iQTM SYBER® Green Supermix (BIO-RAD, USA) with DEPC-Water were added to the synthesized cDNA, followed by CFX96 ™ Real-Time System ) Was used to perform RT-PCR three times. The amount of mRNA expression of Col1a1 was corrected by the expression amount of GAPDH mRNA, and quantitative PCR was performed. The results are shown in Fig.

Example  5. Assessment of collagen degradation inhibitory activity MMP activity )

Human skin fibroblast (Hs68), a human skin fibroblast, was added to a 6-well plate at a concentration of 1 × 10 ⁶ cells / well and cultured at 37 ° C and 5% CO 2 for 24 hours And 1 ml of phosphate buffer saline (PBS) was added to each well plate to wash the cells. As a sample prepared in Example 1, a 20 μg / ml sample of wheat bark extract of the raw material, landscape, algae, Diluted serum-free medium was replaced with culture medium and further incubated for 48 hours (MMP-3). After removing the medium, RNA was isolated using an RNA extraction kit. Then, RT-PCR was performed on the synthesized cDNA to synthesize cDNA and measure mRNA expression level. The primer sequences of GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) and MMP-3 used are shown in the table below.

SEQ ID NO: designation order One GAPDH forward primer 5'-CAA AAG GGT CAT CAT CTC TG-3 ' 2 GAPDH reverse primer 5'-CCT GCT TCA CCA CCT TCT TG-3 ' 3 MMP-3 forward primer 5'-GGT TCC GCC TGT CTC AAG AT-3 ' 4 MMP-3 reverse primer 5'-GGT TCT GGA GGG ACA CCT TC-3 '

1 μL (0.5 μg / μL) of each primer was added to the PCR-premix tube together with DEPC-Water, and quantitative PCR was performed. Real-time PCR system (Mx3000P, Stratagene, Tokyo, Japan) using SYBR PreMix ExTaq (Takara Bio, Shiga, Japan)

The results are shown in FIG. 4, and it was confirmed that the wheat bark extract of all varieties significantly inhibited the synthesis of MMP3.

Example  6. Evaluation of antioxidant effect

DCFDA assays were conducted to evaluate the antioxidative effects of the wheat bark extract samples prepared in Examples 1 and 2. Hacat cells were seeded in 96-well plates at a concentration of 1.5 * 10 ⁵ cells / well and cultured at 37 ° C and 5% CO ₂ for 24 hours. After washing the cells with PBS, the 10 .mu.g / ml or 20 .mu.g / ml sample prepared in Example 1 was replaced with the culture medium and further cultured for 24 hours in DMEM medium containing 10% FBS. Cells were washed with PBS and DCFDA was diluted to 20 μM in PBS medium for 45 min. After washing the cells with PBS, 100 μl of the reagent was added to the medium diluted with PBS to 500 uM in PBS, followed by further incubation for 30 minutes. Then, fluorescence was measured using a microplate reader (Tecan) under Excitation: 485/20, Emission: 528/20. The measured value was converted to the oxidation rate (%) calculated by the following equation (1), and the relative oxidation rate (%) of the wheat extract at 10 占 퐂 / ml when the oxidation rate of glutathione 10 mM as the negative control group was 100% Respectively.

[Equation 1]

&Quot; (2) "

As a result of the measurement of the cell oxidation rate, the results for the wheat bark sample are shown in Figs. 5A and 2, the results for the wheat seed sample are shown in Figs. 5B and 3, and in the samples of all varieties, It was confirmed that the antioxidative effect was superior to the seed part of the wheat and the wheat bark part of the wheat. Specifically, the seed extract of wheat did not lower the antioxidative effect to below 80% of the control group even at the concentration of 40 μg / mL, whereas at the concentration of 20 μg / mL of wheat husk, 60% or 80% of the control group Respectively. This means that there is a difference in activity between the seed part and the wheat bark part and shows the difference in effectiveness of wheat bark part.

sample density Oxidation rate (%) Contrast to glutathione
Relative oxidation rate (%) Control group - 100 Glutathione 10 mM 66.97 100 Scarf 10 μg / ml 70.42 91.49 Scarf 20 μg / ml 53.85 69.97 Landscape shell 10 μg / ml 69.61 90.43 Landscape shell 20 μg / ml 66.05 85.82 Peach shell 10 μg / ml 75.17 97.66 Peach shell 20 μg / ml 73.16 95.05 Pebbles 10 μg / ml 88.40 114.85 Pebbles 20 μg / ml 77.78 101.06

sample density Oxidation rate (%) Contrast to glutathione
Relative oxidation rate (%) Control group - 100 Glutathione 10 mM 59.85 100 Seed seed 10 μg / ml 97.02 162.10 Seed seed 20 μg / ml 86.43 144.41 Seed seed 40 μg / ml 81.37 135.96 Landscape seed 10 μg / ml 123.47 206.29 Landscape seed 20 μg / ml 113.74 190.04 Landscape seed 40 μg / ml 114.77 191.77 Seed Seeds 10 μg / ml 114.51 191.33 Seed Seeds 20 μg / ml 113.16 189.08 Seed Seeds 40 μg / ml 106.83 178.49 Geumgang Seed 10 μg / ml 103.63 173.14 Geumgang Seed 20 μg / ml 89.55 149.62 Geumgang Seed 40 μg / ml 85.63 143.08 Our seed 10 μg / ml 93.02 155.42 Our seed 20 μg / ml 89.60 149.71 Our seed 40 μg / ml 81.34 135.91

Example  7. Evaluation of whitening effect

B16-F10 cells, the melanoma cells of the rats, were divided into 96-well plates at a concentration of 1 * 10 ⁶ cells / well and incubated at 37 ° C and 5% CO 2 until the number of cells increased by 70-80% For 24 hours. After washing the cells with PBS, the 20 μg / ml sample prepared in Example 1 was diluted with DMEM medium containing 10% FBS, replaced with the culture medium, and further cultured for 3 days. After washing the cells with PBS, 200 μl of 0.25% trypsin-EDTA was added and cultured in an incubator for 3 minutes. Then, the cells were desorbed by adding 1 ml of PBS, transferred to a 1.5 ml e-tube, and then the supernatant was removed at 12,000 rpm at 4 ° C for 3 minutes, leaving only the cell pellet. 100 μl of a lysis buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS) And cultured for 30 minutes. Then, the cell pellet and the supernatant were separated by centrifugation at a temperature of 4 캜 and at 13000 rpm for 30 minutes.

 The supernatant was quantitated at 595 nm using BSA stnadard. The cell pellet was dried in an oven at a temperature of 60 ° C, and dissolved in 200 μl of 20% DMSO and 2N NaOH. The absorbance was measured at 405 nm.

The results are shown in FIG. 6, and it was confirmed that whitening effect was excellent in wheat bark extract of all varieties. In particular, it was confirmed that whitening effect was most excellent when 20 ppm of kumquat was treated.

<110> Dongduk Womens's University Industry-Academy Collaboration Foundation <120> Cosmetic composition comprising wheat-chaff extract <130> DPP20170751KR <160> 4 <170> KoPatentin 3.0 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> GAPDH forward primer <400> 1 caaaagggtc atcatctctg 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> GAPDH reverse primer <400> 2 cctgcttcac caccttcttg 20 <210> 3 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MMP-3 forward primer <400> 3 ggttccgcct gtctcaagat 20 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> MMP-3 reverse primer <400> 4 ggttctggag ggacaccttc 20

Claims (9)

A cosmetic composition comprising wheat bark extract as an active ingredient. The cosmetic composition according to claim 1, wherein the wheat husk is at least one selected from the group consisting of chaff and palea. The cosmetic composition according to claim 1, wherein the wheat bark extract is extracted with at least one solvent selected from the group consisting of water, a linear or branched alcohol having 1 to 4 carbon atoms, an organic solvent, and a mixed solvent thereof. The cosmetic composition according to claim 3, wherein the solvent is an aqueous solution of ethanol. The cosmetic composition according to claim 1, wherein a relative oxidation rate (%) of the wheat bark extract is 30 to 135% based on 100% of an oxidation rate of glutathione calculated by the following formula (1)
[Equation 1]

The cosmetic composition according to claim 1, wherein the composition is for improving wrinkles. The cosmetic composition according to claim 1, wherein the composition has collagen biosynthesis promotion activity or collagenase inhibitory activity. The cosmetic composition according to claim 1, wherein the composition is for skin whitening. Separating the wheat husks from the wheat; And
Extracting the wheat husks with at least one solvent selected from the group consisting of water, linear or branched alcohols having 1 to 4 carbon atoms, organic solvents, and mixed solvents thereof.
A method for producing a cosmetic composition comprising wheat bark extract as an active ingredient.

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