KR20180083668A - A cosmetic composition for skin desquamation comprising spicule powder and natural powder - Google Patents

Abstract

The present invention relates to a cosmetic composition, and more specifically, to a cosmetic composition for skin exfoliation which contains: micro-needle powder; and at least one type of natural powder selected from the group consisting of apricot seed powder, wild berry seed powder, walnut shell powder, volcanic ash, and coral powder.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin exfoliating comprising a fine needle powder and a natural powder as active ingredients,

The present invention relates to a functional cosmetic composition containing a micropowder powder and a natural powder.

The stratum corneum is composed of 10 to 20 layers of dead cells and intercellular nerves, natural moisturizing factor (NMF) present in the outermost part of the skin, which protects the skin from external pollutants, Lt; RTI ID = 0.0 > of water < / RTI >

In healthy individuals, the differentiated cells from the basal layer mature and reach the granule layer. On the 14th day, the cells reaching the granule layer disappear and disappear, and it takes 14 days until they become keratinocytes and arrive at and disappear from the stratum corneum. The turn-over period of the skin stratum corneum is about 4 weeks. When a predetermined period elapses, the old stratum corneum is naturally peeled off and a new stratum corneum is formed.

However, due to internal factors such as aging and external factors such as ultraviolet rays, the turnover cycle becomes long and unbalanced, and the stratum corneum is old and stratified, resulting in a rough skin, uneven skin tone, and skin incongruity.

One of the signs of aging skin is a reduction in the ability to dislodge dead cells, which causes the skin to lose vitality and elasticity as the cell regeneration cycle increases. In order to improve this, efforts have been actively made to restore skin elasticity by stimulating cell regeneration of the basal layer by removing the skin layer through a skin peeling technique.

There are three kinds of exfoliating cosmetics. The first is a method of finely cutting the skin by physical action such as skin friction or laser. The second is a chemical method in which an AHA (Alpha-Hydroxy Acid), BHA (Beta- Hydroxy Acid), and urea, and the third is a method of peeling the keratin using a proteolytic enzyme having a function of exfoliation.

In physical removal, skin irritation is severe and it is not suitable for home use. In the case of chemical method, it is not effective because it is possible to use only low volume in household products. Protein peeling agents are expensive and ineffective.

Meanwhile, the cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. In addition to low adverse effects on skin, natural materials have recently been increasingly developed as cosmetic raw materials, as consumers have become more receptive to cosmetics using natural materials.

Accordingly, the present inventors have developed an eco-friendly and highly functional cosmetic product for peeling off keratin which contains only natural ingredients corresponding to EWG 1 grade and can safely use sensitive skin.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a functional cosmetic composition which is less irritating to skin and effectively removes keratin.

According to one aspect of the present invention, there is provided a micropowder powder comprising; And at least one natural powder selected from the group consisting of apricot seed powder, wild strawberry seed powder, walnut shell powder, volcanic ash powder, and coral powder as an active ingredient.

In one embodiment, the micropowder powder may be obtained from a sponge animal.

In one embodiment, the sponge animal may be a Spongilla lacustris .

In one embodiment, the average particle length of the fine needle-like powder may be 60 to 500 탆.

In one embodiment, the micropowder powder may comprise 0.1 to 3% by weight based on the total weight of the composition.

In one embodiment, the natural powder may be a fermentation powder obtained by inoculating a strain of Lactobacillus sp .

In one embodiment, 10 to 20 parts by weight of the apricot seed powder, 10 to 20 parts by weight of the ruby seed powder, 10 to 20 parts by weight of the walnut shell powder, 10 to 20 parts by weight of the powder of the volcanic ash powder, To 20 parts by weight.

In one embodiment, the natural powder may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butyleneglycol .

In one embodiment, the cosmetic composition is a cosmetic composition comprising a composition selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, scrub agent, cleansing cream, cleansing foam, cleansing water, One or more selected from the group consisting of < RTI ID = 0.0 >

The cosmetic composition according to one aspect of the present invention can contain a fine needle powder as an active ingredient, thereby achieving a superior exfoliation effect while minimizing skin irritation. In addition, when one or more natural powders are further added to the fine needle powder, the exfoliation effect of the keratin may be remarkably improved due to the synergistic effect of each component.

In particular, the natural raw material applied to the cosmetic composition of the present invention is a raw material corresponding to the EWG 1 grade and has no harmful effect on the human body, and sensitive skin can be applied safely.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

One aspect of the present invention relates to a micropowder powder; And at least one natural powder selected from the group consisting of apricot seed powder, wild strawberry seed powder, walnut shell powder, volcanic ash, and coral powder as an active ingredient.

The cosmetic composition of the present invention can effectively remove exfoliation of the skin while minimizing skin irritation by containing mixed extract of natural origin as an effective ingredient. In particular, since the mixed extract acts on the skin at the same time, the effect of improving sedative and atopic dermatitis can be remarkably increased due to the synergistic effect.

As used herein, the phrase " comprising as an active ingredient " means containing an effective amount capable of exhibiting a skin improving effect, such as collagen synthesis associated with a wrinkle-reducing effect, elasticity improvement or skin soothing, exfoliating effect .

In particular, since the cosmetic composition contains only EWG level 1 components, it satisfies both functionality and safety, and is easy to apply to sensitive skin because it is not harmful.

EWG is a cosmetic ingredient harmful grade announced by EWG (Environmental Working Group), a nonprofit environmental NGO in the United States. EWG carefully examines the harmfulness of cosmetic ingredients and classifies the hazard level from 1 to 10, and 1 Class 2 and Class 2 are classified as safety class.

The term " exfoliating the skin "means that the keratin substances accumulated on the stratum corneum of the skin are removed or removed from the stratum corneum of the skin. The exfoliation can be performed not only by removing physical force but also by applying a cosmetic composition And the case where it is separated from the stratum corneum.

The term "hypoallergenic" means that the irritation of the skin such as redness and stinging of the skin is relatively reduced. The cosmetic composition includes a fine needle powder and at least one natural powder, so that it can be understood as a cosmetic composition for hypocholesterolemic exfoliation having an excellent exfoliation effect while minimizing skin irritation.

The above-mentioned " Spicule " is a composite material composed of calcium and silicate in the form of various fine needles collected from sponges inhabiting freshwater in Europe and Russia, and can be generally obtained in the form of powder .

The fine needle-like structure is a fine needle-shaped structure and penetrates the skin layer to form a micro channel, so that penetration and keratin separation effect of the applied cosmetic ingredient can be remarkably increased. In addition, the fine needle can promote skin regeneration of collagen which acts on an artificial wound healing, and increase the skin tissue temperature to increase bioactivity.

The fine needle is a harmless substance to the human body and is not particularly limited as long as it is a powdery material capable of stimulating the skin to form fine pores and dissolved or discharged in the body.

In one embodiment, the micropowder powder can be obtained in a spongy animal, wherein the spongy animal is Spongilla lacustris L. , Spongilla fragilis Leidy , May be sponges belonging to the species Ephydatia fluviatilis .

The sponge animal is a primitive marine creature with no muscle, nerves or organs, and more than 5,000 species are present. The skeleton is a tissue that functions as a needle-like skeleton existing in the body of invertebrate animals, and mainly contains silicic acid and calcium carbonate. The sponge animal may comprise uniaxial, triaxial, tetragonal or multiaxial bone fragments containing calcium carbonate or silicic acid as a major component.

The sponge is sponge of various colors such as orange or cyan, and it lives mainly in the sea, but there is also a freshwater sponge.

The sponge animal is an organism that is in the initial stage of tissue in a state where cells are aggregated, and the body is composed of sponge fibers, spicules forming body wall, and cells bound to bone fragments. The main component of the bone fragments is calcite, silicic acid, silica or the like, and the size is about 100 탆 or so and can be observed by a microscope.

The bone fragments can be used as an effective means of skin regeneration to correct visual facial defects such as hyperpigmentation of various etiologies, fine wrinkles, sun damage, superficial scars, cotton wool, and enlarged pores. It is also being used as a natural alternative approach to microdermabrasion and skin erosion, chemical peeling, CO ₂ laser ablation, and erbium laser ablation, which are physical procedures to improve skin conditions.

When the bone fragments come into contact with and rub against the skin layer, they are physically separated to reduce keratinocyte aggregation, and the keratin layer, sebum plug, and keratinocytes can be desorbed to promote regeneration of the skin.

The average particle length of the fine needle-like powder may be 60 to 500 탆. If the average particle length is less than 60 탆, it can not effectively penetrate into the skin to form pores. If it is 500 탆, the dispersibility may be lowered or excessive stimulation to the skin may occur. The fine needle-like powder may contain 0.1 to 3% by weight based on the total weight of the composition. If the fine needle-like powder is less than 0.1% by weight, the exfoliating effect of the keratin may be deteriorated, and if it is more than 3% by weight, excessive skin irritation may be caused.

The above-mentioned " Prunus armeniaca L. seed " belongs to the Rosaceae plant. It stops the cough, sputum and asthma, and it has a lot of greasy and smoothes the bowel. Calcium, Sodium, Fiber, Vitamins A, B, B, C and D are the major components, including amygdalin about 3%, fatty oil about 50%, protein and various free acids, citric acid, malic acid, C and so on.

The above-mentioned " Rubus idaeus seed " belongs to the rose and raspberry, and has an anti-inflammatory effect because it contains a rich tannin component. In addition, it contains polyphenol, which is excellent in anti-inflammation and antioxidant effect, thus preventing and preventing aging. The raspberry seed has a unique fragrance, and can produce a sweet and sweet fragrance.

The "walnut shell ( Juglans regia L. )" is the skin of walnut fruit, the walnut tree is the husk of the seed and the fruit, the leaf contains medicinal substance beneficial to human body, various amino acids and vitamins, . Leafy lobster, the leaf is a gathered leaf with broad oval leaflet like feathers. The fruit is round and has stripes. There are α- and β-hydrouroglons in the husk of unfruitful fruit. There are 60 to 70% of oil, protein, vitamin K, P, B, E and provitamin A, amino acids cystine, glutamine, serine, histamine, valine and phenylalanine. In acupuncture treatment, seeds are used for nourishing tonic medicine, cough medicine, back pain, oil well, coughing, cold cough, and stiffness.

The "volcanic ash " is amorphous inorganic silicate obtained from lava sediments. The volcanic clods (volcanic formations) are the rock fragments that are scattered in the atmosphere due to the impact of volcanic activity or erosion of volcanic gas. Among these, granules of granules are small grains of about 0.25 to 4 mm. Most of the volcanic eruptive rhyolite volcanic rocks are sprinkled in the sky to form the ash. Since the volcanic ash contains a large amount of components necessary for the growth of the plant, it becomes a fertile soil which is good for plants to grow after a long time after the volcanic eruption.

In addition, since the volcanic ash is abundantly contained in a large amount of natural minerals, the cation exchange method is interchanged with the waste materials in the skin, and thus the skin cosmetic effect is excellent. Fine particles of the volcanic ash removes aging keratin, specifically removing fine wastes and residues from the pores with excellent adsorptive power, and thus has excellent pore cleaning effect, and also has an effect on improving skin complexion and improving the complexion brightness.

The "Coral (Coral)" are sometimes collectively referred to as the flower of a tonic to the animal's color is gorgeous ocean belonging to anthozoa sanhogwa. The coral can only live in clear and clean seas, and when it is grown, it is transported to the seabed and deposited deep into the sea floor. The corals that have been transported to the sea floor are decomposed with time, and only the minerals that make up the skeleton are deposited and the sediments are coral (fossil).

The coral contains fine pores and contains a large amount of minerals, so that the interaction, specifically, the absorption and exclusion are crossed, and when applied to the skin, the coral balance and the water balance are adjusted and the skin temperature is lowered have. It is excellent in the removal of wastes from the pores, and has excellent skin moisturizing and elasticity improving effects.

The natural powder can exhibit an excellent exfoliation effect due to interaction between active ingredients derived from each raw material. The natural powder is applied to the skin together with various components contained in each powder, thereby increasing the absorption rate and maximizing the exfoliating effect.

The "natural powder" means that the active ingredient contained in the raw material is brought into contact with the solvent and the raw material under specific conditions to obtain a solvent, followed by further processing such as filtration, vacuum distillation or freeze-drying.

In detail, the natural powder may be extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, The alcohol may be ethanol at a concentration of 60 to 90% (v / v).

The extraction ratio of the active ingredient contained in the raw material may be different depending on the polarity of the solvent. Since the ethanol is excellent in selectivity for extracting a physiologically active substance from a natural raw material, the ethanol extract can realize an optimal skin improving effect .

Particularly, the polarity of water and ethanol is different, so that the effective ingredient to be extracted according to each polarity can be changed, and the concentration of ethanol can be appropriately controlled so that an optimal skin improving effect can be realized. At this time, if the concentration of the ethanol exceeds 90%, an adequate yield may not be realized. If the concentration is less than 60%, the effective ingredient showing skin improving effect may not be extracted sufficiently.

Each of the raw materials is washed with water, dried, and pulverized. The natural powder is recovered by a conventional method such as reflux circulation extraction, pressure extraction, and ultrasonic extraction for about 1 hour to 24 hours with a solvent having a volume of 8 to 12 times the weight of the raw material Extraction and filtration, followed by further processing such as vacuum distillation or freeze-drying.

The natural powder may also contain an extract which has been subjected to a conventional purification process. For example, the extract may be subjected to various purification methods such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity) Lt; RTI ID = 0.0 > fractions. ≪ / RTI >

Wherein the cosmetic composition comprises 10 to 20 parts by weight of the apricot seed powder, 10 to 20 parts by weight of the virgin seed powder, 10 to 20 parts by weight of the walnut shell powder, 10 to 20 parts by weight of the ash powder, Parts by weight.

In the case where the content deviation between the respective active ingredients constituting the natural powder is out of the above range, the synergistic effect due to the interaction between the active ingredients may not be properly realized. Therefore, The ratio can be appropriately controlled.

On the other hand, the natural powder may be a fermentation powder obtained by inoculating a strain of Lactobacillus sp .

The above-mentioned " fermentation powder " is prepared by diluting the powder to a certain concentration in a solvent, preferably purified water, and then inoculating a microorganism for fermentation, that is, a Lactobacillus sp. Strain and fermenting the mixture to a rotary vacuum concentrator and a freeze dryer And can be produced in powder form.

The Lactobacillus genus strains Lactobacillus pento suspension (Lactobacillus pentosus), Lactobacillus brevis (Lactobacillus brevis), Lactobacillus Planta room (Lactobacillus plantarum), Lactobacillus casei (Lactobacillus casei) or Lactobacillus know also required Ruth (Lactobacillus acidophilus , but are not limited thereto.

The cosmetic composition may be at least one selected from the group consisting of softening agents, convergent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, scrub agent, cleansing cream, cleansing foam, cleansing water, / RTI >

The cosmetic composition may be formulated by a conventional method. The contents disclosed in Remington ' s Pharmaceutical Sciences, Mack Publishing Company, Easton PA in the formulation of the external preparation for skin can be referred to, and in the formulation of the cosmetic composition, International cosmetic ingredient dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared into a general emulsified formulation and a solubilized formulation. For example, lotion such as soft lotion or nutrition lotion; Lotion such as facial lotion, body lotion; Nourishing cream, moisture cream, cream such as eye cream; essence; Makeup ointment; spray; Gel; pack; Sunscreen; Makeup base; A foundation such as a liquid type, a solid type or a spray type; powder; Make-up removers such as cleansing creams, cleansing lotions and cleansing oils; Or a cleansing agent such as a cleansing foam, a soap, a body wash, and the like, but is not limited thereto. The external preparation for skin may be formulated into ointments, patches, gels, creams or sprays, but is not limited thereto.

The cosmetic composition of the present invention may be appropriately blended with other ingredients within the range of not compromising the object of the present invention depending on the kind of the formulation or the purpose of use, in addition to the essential ingredients in each formulation.

The cosmetic composition may contain a conventional acceptable carrier and may appropriately contain, for example, oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a colorant, no.

The acceptable carrier may vary according to the formulation. For example, when formulated into ointments, pastes, creams or gels, there may be used an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide, Mixture can be used

When the cosmetic composition is formulated into a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component. In the case of a spray, Propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, 1,3-butyl glycol oil may be used, and in particular, fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When formulated into a suspension, the cosmetic composition may contain, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the cosmetic composition is formulated into soap, it is preferable to use, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, Can be used.

The cosmetic composition may be a cosmetic or dermatological composition which is generally used in the art depending on the quality or function of the final product, such as a lipid, an organic solvent, a solubilizing agent, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, Surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the field of cosmetics or dermatology, such as any other ingredient.

However, the adjuvant and the mixing ratio thereof can be appropriately selected so as not to affect the preferable properties of the cosmetic composition according to the present invention.

The present invention will be further described with reference to the following examples, but it should be apparent that the present invention is not limited by the following examples.

Production Example 1: Preparation of fine needle powder

800 g of 0.5 N sodium hydroxide solution was added to 200 g of the natural sponge obtained from the dried sponge ( Spongilla lacustris ), and the mixture was mixed and pulverized by an ultrasonic process. After stirring, impurities were removed by centrifugation and 700 ml of purified water was added to remove soluble impurities and sodium hydroxide solution.

A small amount of sodium hydroxide was removed by chemical reaction with 800 ml of a 2% -L-ascorbic acid solution, and then the charged L-ascorbic acid solution was removed, followed by drying to obtain fine needle powder from which purified water was removed.

Production Example 2: Preparation of natural powder

Dried apricot seeds, raspberry seeds, walnut shells, volcanic ash, and coral were thoroughly dried at room temperature and pulverized to obtain 100 g of pulverized products, respectively.

100 g of each pulverized product was immersed and extracted with a 10-fold volume of an aqueous ethanol solution (70% mass concentration) as a solvent at a temperature of 70 ° C for 12 hours.

The extract was filtered with 250 mesh and 0.5 mu m filters and then extracted three times in the same manner for the remaining raw materials. The extract was concentrated under reduced pressure at 30 DEG C and lyophilized to obtain a powder.

Preparation Example 3: Preparation of fermentation powders

Each of the powders obtained in Production Example 2 was diluted to 5% by weight with purified water, and then fermented for 12 hours by inoculating Lactobacillus plantarum, a fermentation strain.

The fermented extract was filtered with a 500 mesh and 0.2 mu m filter and dried to obtain each fermentation powder

Examples and Comparative Examples

In order to verify the skin-improving effect of the obtained fine needle powder, natural powder and fermented powder, examples and comparative examples were set by mixing samples as shown in Table 1 below.

As shown in Table 1, the raw materials were mixed at a predetermined ratio or the peel-off effect of the micropowder powder according to the average particle length was examined.

Examples 1 to 6 and Comparative Examples 1 to 4 each had a thickness of 400 μm, and Comparative Examples 5 and 6 contained fine needle powder of 20 μm and 700 μm, respectively.

[Content (parts by weight)] division Example
One Example
2 Example
3 Example
4 Example
5 Example
6 Comparative Example
One Comparative Example
2 Comparative Example
3 Comparative Example
4 Comparative Example
5 Comparative Example
6 Fine needle powder 150 100 50 150 100 50 300 - - - 50 50 Apricot seed powder 150 - 50 - - - - 300 - - 50 - Raspberry seed powder - 100 50 - - - - - - - 50 - Walnut shell powder - - 50 - - - - - - - 50 - Ash powder - 100 50 - - - - - - - 50 - Coral powder - - 50 - - - - - - - 50 - Apricot
Fermented powder - - - 150 - 50 - - - - - 50 Raspberry
Fermented powder - - - - 100 50 - - - - - 50 Walnut shell
Fermented powder - - - - - 50 - - 300 - - 50 Ash
Fermented powder - - - - 100 50 - - - - - 50 Coral fermented powder - - - - - 50 - - - 300 - 50

Experimental Example 1: Skin safety test

MTT assay experiments were performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) to verify skin safety for the samples of the Examples and Comparative Examples.

The samples of Examples and Comparative Examples were each suspended in purified water at a concentration (50 mg / L), and cell viability was measured.

Cytotoxicity was performed by modifying the method of Mosmann to measure cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

(HDF), melanocyte-forming cells (B16F10), and keratinocytes (HaCaT) were dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and cultured at 37 ° C. and 5% CO ₂ for 48 hours Respectively.

After the culture medium was removed and the sample was cultured for 48 hours in a concentration-treated medium, the medium was removed and repeatedly washed with PBS (phosphate buffered saline). MTT was dissolved in PBS at 5 mg / mL and 50 L was added. The cells were cultured at 37 ° C and 5% CO ₂ for 24 hours. 100 L of DMSO (dimethyl sulfoxide) was added to each well, stirred for 10 minutes, and absorbance was measured at 540 nm.

As a result of measurement, cytotoxicity was not confirmed at all concentrations of each sample. The above results suggest that the sample of the composition is not only harmless to human body but also has excellent human safety.

Experimental Example 2: Test for promoting keratinocyte differentiation

Human keratinocytes were cultured in a culture flask and grown to about 80%. The samples of the examples and comparative examples were added at 200 ppm concentrations as shown in the following Table 2 and treated for 4 days.

SDS (sodium dodecyl sulfate) as a denaturant and β-mercaptoethanol as a reducing agent were mixed to remove dissolved proteins.

The concentration of keratinocytes was measured by measuring the absorbance of the remaining stratum corneum at 310 ㎚. The amount of keratinocytes was compared with that of the negative control (0%). At this time, TGF-beta (10 nM), a cell signaling substance, was treated as a positive control.

division Promoting effect of keratinocyte differentiation (%) Example 1 132.5 Example 2 139.4 Example 3 147.3 Example 4 136.7 Example 5 143.6 Example 6 154.2 Comparative Example 1 37.6 Comparative Example 2 29.4 Comparative Example 3 55.4 Comparative Example 4 59.1 Comparative Example 5 111.9 Comparative Example 6 105.6 Positive control group 143.5

The results are shown in Table 2. Referring to Table 2, the samples of Examples 1 to 6 were excellent in promoting keratinocyte differentiation, and the samples of Examples 4 to 6 containing fermentation powders were remarkably improved in the effect of promoting keratinocyte differentiation. In addition, in the case of the sample containing the fine needle powder having an average length of the effective range, the effect promoting keratinocyte differentiation promotion was remarkably improved as compared with the sample containing unnecessarily small or large fine needle powder.

In particular, the sample of Example 6 exhibited significantly improved keratinocyte differentiation promoting effect as compared with TGF-beta, a cell signaling substance.

Experimental Example 3: Skin moisturizing effect test

The skin moisturizing power improving effect of the cosmetic compositions containing the samples of Examples and Comparative Examples was evaluated. Using the samples of Examples and Comparative Examples, scrub type cosmetic compositions of the same composition were prepared. Each sample was formulated into a scrubbing agent having the same composition, except that the samples of Examples 1 to 6 and Comparative Examples 1 to 6 were different.

In order to measure the skin moisturizing effect of the scrub type cosmetic composition, 100 subjects (20 to 40 year old female) were divided into 10 groups of 10 persons, and samples of the examples and comparative examples were included in the entire face for each group Was used for 3 consecutive weeks for 1 month.

The skin conductivity was measured in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application and the values were measured at 1 week, 2 weeks, The skin conductivity increase rate (%) was measured and evaluated. The results are shown in Table 3 below.

division 1 week 2 weeks 4 weeks Example 1 25 30 42 Example 2 31 35 48 Example 3 34 47 55 Example 4 33 41 48 Example 5 35 46 57 Example 6 42 56 59 Comparative Example 1 13 15 18 Comparative Example 2 15 18 18 Comparative Example 3 14 15 17 Comparative Example 4 17 19 22 Comparative Example 5 22 25 26 Comparative Example 6 18 20 22

Referring to Table 3, the experimental group to which the cosmetic composition of Examples 1 to 6 was applied exhibited an excellent skin conductivity increase rate as compared with Comparative Examples 1 to 6. Particularly, the cosmetic compositions of Examples 4 to 6 containing the fermentation powders had an excellent skin conductivity increasing effect, and the cosmetic composition containing the micronized needle powder having an average length of the effective range had an unnecessary small or large fine needle powder , The effect of increasing skin conductivity was remarkably excellent.

Since the skin conductivity is in proportion to the skin moisture content, it was confirmed from the above results that the cosmetic compositions containing the samples of Examples 1 to 6 kept the skin moisture content higher than those of the cosmetic compositions containing the samples of the comparative examples.

Experimental Example 4: Skin elasticity improvement effect test

Using the samples of Examples and Comparative Examples, scrub type cosmetic compositions of the same composition were prepared. Each of the above samples was formulated into a scrubbing agent having the same composition, but the samples of Examples 1 to 6 and Comparative Examples 1 to 6 were different.

In order to measure the skin elasticity-improving effect of the scrub-type cosmetic composition, 100 subjects (20 to 40 years old) were divided into 10 groups each consisting of 10 subjects. A scrub agent containing samples for each group and a comparative sample was divided into 10 groups, 3 times a week for 1 month continuously.

In order to confirm the improvement of skin elasticity, skin elasticity was measured before use and 2 months after using the skin elasticity meter (SEM 575, C + K Electronic Co., Germany). The experimental results are reported as the R7 value of Cutometer SEM 575, and the average value of each group is shown in Table 4 below. The R7 value indicates the nature of the viscoelasticity of the skin (n = 10, p < 0.05).

division R7 Example 1 0.35 Example 2 0.38 Example 3 0.43 Example 4 0.46 Example 5 0.54 Example 6 0.63 Comparative Example 1 0.09 Comparative Example 2 0.15 Comparative Example 3 0.12 Comparative Example 4 0.18 Comparative Example 5 0.22 Comparative Example 6 0.24

Referring to Table 4, the samples of Examples 1 to 6 effectively improved skin elasticity in the facial area.

Particularly, when the cosmetic composition of Example 6 including the mixed fermentation powder was treated, the elasticity of the facial region was remarkably improved.

Experimental Example 5: Evaluation of skin irritation

A skin patch test was conducted to examine the skin irritation test for each of the scrub type cosmetic composition. The subjects were selected as persons without skin disease at the test site of 15 to 30 years old.

200 subjects were divided into 10 groups of 20 persons each. The upper part of the pellet was wiped with 70% ethanol and dried. Then, 0.1 g of the product was applied for 24 hours, the skin reaction was checked within 1 hour after removal of the patch, and the next day (48 hours later). Skin response determinations were made according to the following test criteria. The skin patch test results are shown in Table 5.

division Judgment result - ± + ++ +++ Example 1 12 2 2 3 One Example 2 12 5 One One One Example 3 14 3 2 - One Example 4 15 2 - 2 One Example 5 17 3 - One - Example 6 18 One One - - Comparative Example 1 2 4 3 5 6 Comparative Example 2 4 5 6 4 One Comparative Example 3 5 4 6 2 3 Comparative Example 4 4 7 4 4 One Comparative Example 5 7 4 2 3 4 Comparative Example 6 8 4 5 3 -

* Skin response test criteria / -: No reaction / ±: weak positive (erythema) / +: positive (erythema) / ++: strong positive (erythema, edema) / +++: severe positive , Swelling, blistering)

Referring to Table 5, the scrub formulation cosmetics formulated with the samples of Examples 1 to 6 hardly induced skin irritation as compared with Comparative Examples 1 to 6. [

Experimental Example 6: Sensibility Evaluation

The above scrub type cosmetic composition was subjected to sensory evaluation.

A total of 100 people, aged between 20 and 40, were applied to each of the ages, and the above-described scrub agent was applied to the right and left hands, and then the mass was applied to each of the massagers to measure gel cohesion, skin irritation, .

 The highest score is 10 points, the average score is 5 points, and the score is 0 points. The score below is the average of 100 responses.

The results of sensory evaluation according to the above Test Example are shown in Table 6 below.

Evaluation items Gel cohesiveness Degree of exfoliation Skin irritation Skin moisturizing power Overall likelihood Example 1 7 7 7 6 7 Example 2 8 7 9 7 7 Example 3 9 8 8 7 8 Example 4 8 8 8 7 7 Example 5 7 8 8 8 8 Example 6 9 9 9 9 9 Comparative Example 1 4 3 3 5 2 Comparative Example 2 5 5 5 6 3 Comparative Example 3 4 6 5 5 6 Comparative Example 4 5 5 6 5 5 Comparative Example 5 6 7 5 6 6 Comparative Example 6 6 7 3 6 6

Referring to Table 6, it was evaluated that the scrub form cosmetics formulated with the samples of Examples 1 to 6 were significantly superior to the cosmetic compositions of Comparative Examples 1 to 6 as a whole.

In particular, all of the evaluated items, such as gel cohesion, exfoliation effect, skin irritation, and moisturizing ability, were evaluated to be high.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (9)

Fine needle powder; And
Wherein the cosmetic composition comprises at least one natural powder selected from the group consisting of apricot seed powder, wild strawberry seed powder, walnut shell powder, volcanic ash powder, and coral powder as an active ingredient. The method according to claim 1,
The fine needle powder is obtained from a sponge animal. 3. The method of claim 2,
Wherein the sponge animal is Spongilla lacustris . The method according to claim 1,
Wherein an average particle length of the fine needle-like powder is 60 to 500 탆. The method according to claim 1,
Wherein the fine needle powder comprises 0.1 to 3% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the natural powder is a fermentation powder obtained by inoculating a strain of Lactobacillus sp . The method according to claim 1,
10 to 20 parts by weight of the apricot seed powder, 10 to 20 parts by weight of the wild strawberry seed powder, 10 to 20 parts by weight of the walnut shell powder, 10 to 20 parts by weight of the powder of the ash powder, and 10 to 20 parts by weight of the coral powder Cosmetic composition. The method according to claim 1,
Wherein the natural powder is extracted with at least one solvent selected from the group consisting of purified water, a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. 9. The method according to any one of claims 1 to 8,
At least one selected from the group consisting of softening lotion, astringent lotion, nutritional lotion, lotion, nutritional cream, massage cream, essence, eye cream, scrub, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder Cosmetic composition.